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Supplemental Fig. 1
Immunogold detection of mannans in vascular bundle cells of the apical stem labeled by LM22. a, b
Labeling was initially detected in metaxylem vessels (V) during S2 formation and no labeling was detected
in xylary fibers (F) at the S1 formation stage. Note specific labeling in cytoplasm. c, d Protoxylem vessels
(pV) showed much stronger labeling than V with strong labeling in outer cell wall regions. Labeling in pV
was much lower than LM21 (Fig. 2e, f). Ca, cambium. Bars = 500 nm
Supplemental Fig. 2
Immunogold detection of mannans in parenchymatous cells of the basal stem labeled by LM21. No specific
labeling was detected in the cell wall of any parenchymatous cells. Bars = 500 nm
Supplemental Fig. 3
Supplemental Fig. 3 continued
Immunogold detection of pectic homoglacturonan (HG) in basal stem labeled by LM19. Strong labeling
was detected in the cell wall of all parenchymatous cells (a–f). Labeling in vascular cells was limited in the
middle lamella regions (g, h). F, xylary fiber; V, metaxylem vessels. Bars = 500 nm
Supplemental Fig. 4
Immunogold detection of pectic homoglacturonan (HG) in the basal stem after pectinase treatment labeled
by LM19. Labeling was significantly reduced in all cell types after pectinase treatment (Supple. Fig. 2). F,
xylary fiber; V, metaxylem vessels. Bars = 500 nm