Supplemental Figure S1: Representative sections showing changes in expression between baseline biopsy and post-treatment surgical specimens

Baseline Post surgery

p70S6K

pAKT

pS6

Baseline Post surgery

p4EBP1

p-eIF4e

p-mTOR

BIM

Light microscopic appearance of immunohistochemistry stains showing reduced expression of pS6 and p-p70S6K and increased p-Akt expression (x400) and reduced expression of p4EBP1, increased eIF4e, no change in p-mTOR and increased BIM (x200) in the post-treatment surgical specimens in comparison to the corresponding baseline biopsy specimens in a patient treated with everolimus.

Supplemental Figure S2: Correlogram showing the correlation between changes in protein expression immunoscore, metabolic activity on PET and tumor size by CT scan

Correlogram with pairwise correlations between change in protein expression in paired tissue samples, change in metabolic change on PET and change in tumor diameter on CT scan. The main diagonal lists the covariates, tested biomarker, SUVmax change and change in tumor diameter. Pearson correlation coefficient (center) and the associated p-value (top corner) are indicated at the horizontal and vertical intersection of the covariates,