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Supplementary Figure 1
a
b
RANK
1 2 3 4 5 6 7 8 9 10 11
*
RA
NK
Arb
itra
ry S
core
ns
0
1
2
3
Normal breast Primary tumor Metastasislymph node
*
a c
d
Supplementary Figure 2
Day 3Day 1
3 H t
hym
idin
e u
pta
ke
(CP
M)
0
40000
60000
80000
100000
120000
20000
RANKL
RANKL / OPG
Control
Aniso
myc
in
Contr
ol
UV
Sorb
itol
% s
urv
ival
0
10
20
30
40
50
60 RANKLControl
RANKL Prolactin pERK1/2
pAKTAKT
pSTAT5 STAT5
0 5 10 20 40 min 5 10 20 40
pERK1/2
-Actin
ERK1/2
RANKL
0 5 10 30 60 minRANK
b
e
Supplementary Figure 3
% in
cre
ased
mig
rati
on
RANKL [1
g/m
l]
RANKL [2
.5g/
ml]
SDF-1
[0.1g
/ml]
SDF-1 [0
.1g/
ml]
RANK
L [2.5g
/ml]
SDF-1 [1
g/m
l]
SDF-1 [1
g/m
l]
R
ANKL [2
.5g
/m]
0
20
40
60
80
100
120
a
b c
Mig
rati
on
tow
ard
th
e ti
p, m
m
-40
0
40
80
120
160
Con
trol
RA
NK
LR
AN
KL
OPG
*
Supplementary Figure 4
Control RANKL RANKL
Control RANKL
RANK
-actin
1 2
c
RA
NK
LR
AN
KL /
OPG
RA
NK
L / -
CX
CR
4SD
F-1
-C
XC
R4
SDF-1
O
PG
SD
F-1
10
20
30
40
50
60
70
0
% in
crea
sed
mig
rati
on
* *
Supplementary Figure 5
a
ERK1/2
RANKL SDF-1
0 3 10 30 60 120 min0 3 10 30 min
pERK1/2
d
b
0
10
20
30
40
50
60
70
80
90
100
% m
igra
tion
% in
crea
sed
mig
rati
on
20
40
60
80
100
0
SDF-
1
RA
NK
L6C
kine
6Cki
ne /
RA
NK
LC
TA
CK
CT
AC
K /
RA
NK
LSD
F-1
/ R
AN
KL
**
Supplementary Figure 6
a c
Tibia Vertebra
Tumor Control
b
*
0
100
200
300
400
500
600
700
Total
Trabec
ular
Cortica
l
Bon
e d
ensi
ty (
pQ
CT
mg/
cm3 )
Control
B16F10
Supplementary Figure 7
Control Tumor Tumor / OPG
**
a b c
d e f
g h i
**
