Supplementary Table 1.Strain List species strain Genotype Source

NcW#1 △His-3 ;△ads-4 this studyNcW#2 △ads-4 ;ads-4 this studyads-4 OE OE::ads-4 this studyWT wild type FGSCFGSC #11386 △ads-4 FGSCFGSC #12544 △Ncmmt-2 FGSCFGSC #13802 △erg5 FGSCFGSC #21286 △NcabcB FGSCFGSC #11238 △cdr4 FGSCFGSC #13302 △Ncmnn4 FGSCFGSC #13399 △Ncrta2 FGSCYJ407 wild type Jin cheng labCEA17 △pyrG89 Jin cheng labAfu1g16460 △Afads4 this study

Neurospora crassa

Aspergillus fumigatus

Supplementary Fig. 1 Sequence alignment of ADS-4 homologs. Sequence

alignment was performed using clustalx 1.83 and conserved amino acids were

shaded. FOXG_11153, FVEG_09636, FGSG_01555, MGG_03288,

Afu1g16460, and ANID_11891 were homologs of ADS-4 in Fusarium

oxysporum, Fusarium verticilloides, Fusarium graminearum, Magnaporthe

oryzae, Aspergillus fumigatus, and Aspergillus nidulans, respectively.

Supplementary Fig. 2 Phylogenic analysis of NcAbcB and its homologs in Aspergillus fumigatus and Saccharomyces cerevisiae. The neighbor-joining tree was constructed using MEGA version 6 with cut-off bootstrap values of 50% obtained from 1000 replicates (NCU=Neurospora crassa, Afu=Aspergillus fumigatus, YDR= Saccharomyces cerevisiae).