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Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop Laser Microdissection (LMD)

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Page 1: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Laser Microdissection (LMD)

Page 2: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Instructors

Chicago Medical School (RFUMS)Christine Sanders [email protected]

Northern Louisiana Crime Lab (NLCL)Pat Wojtkiewicz [email protected] Kelli Raley [email protected]

Leica MicrosystemsAndy Lee [email protected]

Page 3: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Student Introductions

Page 4: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Facilities• Where are we?• Largo, FL• NFSTC - National Forensic Science Technology Center

• http://nfstc.org/aboutus/index.htm

• Walking tour

Page 5: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Agenda ReviewDAY 1• Introduction to LMD technology• Basic cell identification• Preparing samples for LMD (lab)• Demonstration and instruction of LMD

instrumentation (lab)• LMD exercises (lab)• (NFSTC staff performs PCR and CE)

Page 6: Technology Transfer Workshop Laser Microdissection (LMD)

Technology Transfer Workshop

Agenda Review cont…DAY 2• Research studies in forensic LMD

development• LMD applications using fluorescence (lab)• LMD experiences in the forensic laboratory• Review of DNA results from “DAY 1”

laboratory exercises• Practical application of LMD in the forensic

laboratory• TL/Managers only - Round table discussions