The Effects of Resveratrol and Melatonin on Drosophila melanogaster with Paraquat

Induced Oxidative Stress

By Anthony Anastasio

Need

Figure 1Over the passed 6 years, disorders from alcohol have increased

Figure 2 Wine still remains the leased consumed alcoholic beverage

Oxidative stress

Antioxidants

Resveratrol

• Found in the skin of grapes that are used in the fermentation of red wine.

• Found in plants as a defense system against disease.

Bass (2007)

• When drosophila exposed to resveratrol, no increase in lifespan is shown

Effect of Resveratrol on the Life span of C. Elegans

Bauer (2004)• Drosophila showed an

increase in lifespan with resveratrol supplements

Bonilla and Molero (2006)• Drosophila exposed to

Melatonin, Serotonin, Lipoic acid, and Ascorbic acid

The survivability of Drosophila shown after paraquat induced oxidative stress with different pre exposures

Purpose

• To determine the effects of resveratrol and melatonin on the survival rate of drosophila after paraquat induced oxidative stress.

Hypothesis

• Null Hypothesis- Drosophila will have the same survival rate after paraquat induced oxidative stress in all groups and no change in life span will occur.

• Alternative Hypothesis- Drosophila that are pre-exposed to resveratrol and melatonin will have a higher survival rate after paraquat induced oxidative stress and a longer life span then the control group.

Control Resveratrol0.2mM

Resveratrol1.0mM

Melatonin0.22mM

Melatonin0.43mM

Fed drosophila cornmeal medium

Exposed to 0.2 mM of resveratrol in

food

Exposed to 1.0 mM of resveratrol in

food

Exposed to 0.22 mM of Melatonin

in food

Exposed to 0.43 mM of Melatonin

in food

Drosophila exposed to Paraquat induced oxidative stress with filter paper soaked in 20mM of paraquat

Life span of the Drosophila was tested

The Effects of Resveratrol and Melatonin on Drosophila melanogaster with Paraquat Induced Oxidative Stress

Survival rate tested in the drosophila and recorded after 24 hours and 48 hours after

exposure to paraquat

Data put into SPSS for a One-way ANOVA and Scheffe post-hoc test

Budget

Vendor Cat.# Page Describe Item Quantity Unit Price Total Price

Sigma-Aldrich online R5010-100mg   Resveratrol 1 81.50  81.50

Science lab, chemicals and laboratory equipment  SLD1582-1G   Paraquat 1 282.12  228.12

Sigma-Aldrich online M5250-1G    Melatonin 1 54.00  54.00

       

Do-ability

• Drosophila cultures are available• Exposure to Resveratrol and Melatonin • Procedures that been done in lab