MINIREVIEWS

2221 No Splicing, No Dicing: Non-proteolytic Roles of the Ubiquitin-Proteasome System in Transcription. Thomas Kodadek

ACCELERATED PUBLICATIONS

2227□S

Direct and Indirect Induction by 1,25-Dihydroxyvitamin D3 ofthe NOD2/CARD15-Defensin �2 Innate Immune PathwayDefective in Crohn Disease. Tian-Tian Wang, Basel Dabbas, DavidLaperriere, Ari J. Bitton, Hafid Soualhine, Luz E. Tavera-Mendoza, SergeDionne, Marc J. Servant, Alain Bitton, Ernest G. Seidman, Sylvie Mader,Marcel A. Behr, and John H. White

DNA: REPLICATION, REPAIR, RECOMBINATION, ANDCHROMOSOME DYNAMICS

2351 Mechanism of Action of an Imidopiperidine Inhibitor of HumanPolynucleotide Kinase/Phosphatase. Gary K. Freschauf, Rajam S.Mani, Todd R. Mereniuk, Mesfin Fanta, Caesar A. Virgen, Grigory L.Dianov, Jean-Marie Grassot, Dennis G. Hall, and Michael Weinfeld

2632□S

Characterization of the Mycobacterial AdnAB DNA MotorProvides Insights into the Evolution of Bacterial Motor-Nuclease Machines. Mihaela-Carmen Unciuleacand Stewart Shuman

2876□S

Uracil DNA Glycosylase Activity on Nucleosomal DNA Dependson Rotational Orientation of Targets. Hope A. Cole, Jenna M.Tabor-Godwin, and Jeffrey J. Hayes

TRANSCRIPTION, CHROMATIN, AND EPIGENETICS

2258□S

Regulation of SIVmac239 Basal Long Terminal Repeat Activityand Viral Replication in Macrophages. FUNCTIONAL ROLES OFTWO CCAAT/ENHANCER-BINDING PROTEIN � SITES IN ACTIVATIONAND INTERFERON �-MEDIATED SUPPRESSION. Shruthi Ravimohan,Lucio Gama, Sheila A. Barber, and Janice E. Clements

2340□S

The SWI/SNF Chromatin-remodeling Complex ModulatesPeripheral T Cell Activation and Proliferation by ControllingAP-1 Expression. Seung Min Jeong, Changjin Lee, Sung Kyu Lee,Jieun Kim, and Rho Hyun Seong

2361□S

A Bre1-associated Protein, Large 1 (Lge1), Promotes H2BUbiquitylation during the Early Stages of TranscriptionElongation. Young-Ha Song and Seong Hoon Ahn

2375□S

Regulation of Pyruvate Dehydrogenase Kinase 4 (PDK4) byThyroid Hormone. ROLE OF THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR � COACTIVATOR (PGC-1�). Ramy R. Attia, SaraConnnaughton, Lindsey R. Boone, Fang Wang, Marshall B. Elam, GeneC. Ness, George A. Cook, and Edwards A. Park

2438�□S

Activator Gcn4 Employs Multiple Segments of Med15/Gal11,Including the KIX Domain, to Recruit Mediator to Target Genesin Vivo. Iness Jedidi, Fan Zhang, Hongfang Qiu, Stephen J. Stahl, IraPalmer, Joshua D. Kaufman, Philippe S. Nadaud, Sujoy Mukherjee,Paul T. Wingfield, Christopher P. Jaroniec, and Alan G. Hinnebusch

2545□S

Ikaros Silences T-bet Expression and Interferon-� Productionduring T Helper 2 Differentiation. Rajan M. Thomas, ChunxiaChen, Neelanjana Chunder, Lingzhi Ma, Justin Taylor, Edward J.Pearce, and Andrew D. Wells

2601 Complex Regulation of the Transactivation Function ofHypoxia-inducible Factor-1� by Direct Interaction with TwoDistinct Domains of the CREB-binding Protein/p300. Jorge L.Ruas, Utta Berchner-Pfannschmidt, Sohail Malik, Katarina Gradin,Joachim Fandrey, Robert G. Roeder, Teresa Pereira,and Lorenz Poellinger

2622□S

Nuclear Factor 1 Synergizes with Progesterone Receptor on theMouse Mammary Tumor Virus Promoter Wrapped around aHistone H3/H4 Tetramer by Facilitating Access to the CentralHormone-responsive Elements. Guillermo Pablo Vicent, RoserZaurin, A. Silvina Nacht, Jofre Font-Mateu, Francois Le Dily,and Miguel Beato

On The CoverThe cover shows the likely binding site for the activator protein Gcn4 on the surface of the KIX domain of the Gal11/Med15 subunit of theMediator transcriptional coactivator complex in yeast Saccharomyces cerevisiae, mapped using NMR spectroscopy. KIX residues experiencingmoderate and large amide NMR chemical shift changes upon the addition of Gcn4 are highlighted in yellow and red, respectively. In thebackground small regions of two-dimensional NMR spectra of the KIX domain, corresponding to residues Gly-36 –Ser-38 found in the loopconnecting helices 1 and 2, recorded as a function of increasing Gcn4 concentration are shown as red, green, blue, and magenta contours. Fordetails see the article by Jedidi et al., pages 2438 –2455.

The Journal of Biological ChemistryThis issue was available in electronic form on January 15, 2010. www.jbc.orgCopyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc9650 Rockville Pike, Bethesda, MD 20814 U.S.A.

TABLE OF CONTENTSCONTENTS ARE ARRANGED BY SUBJECT CATEGORIESFull Instructions to Authors are found on the web at www.jbc.orgJBCHA3 285(4) 2221–2896 (2010)

� Paper of the Week Author’s Choice □S Online version contains supplemental material □O Available in online journal only

JANUARY 22, 2010 • VOLUME 285 • NUMBER 4 JOURNAL OF BIOLOGICAL CHEMISTRY vii

2642□S

Glucocorticoid Regulation of Mouse and Human DualSpecificity Phosphatase 1 (DUSP1) Genes. UNUSUAL CIS-ACTINGELEMENTS AND UNEXPECTED EVOLUTIONARY DIVERGENCE. CarmenR. Tchen, Joana R. S. Martins, Nasren Paktiawal, Roberta Perelli, JeremySaklatvala, and Andrew R. Clark

2676□S

Estrogen-mediated Regulation of Igf1 Transcription andUterine Growth Involves Direct Binding of Estrogen Receptor �to Estrogen-responsive Elements. Sylvia C. Hewitt, Yin Li, LepingLi, and Kenneth S. Korach

2695□S

The C53/C37 Subcomplex of RNA Polymerase III Lies Near theActive Site and Participates in Promoter Opening. George A.Kassavetis, Prachee Prakash, and Eunjung Shim

PROTEIN SYNTHESIS, POST-TRANSLATIONALMODIFICATION, AND DEGRADATION

2368 ERK Regulates Calpain 2-induced Androgen ReceptorProteolysis in CWR22 Relapsed Prostate Tumor Cell Lines.Honglin Chen, Stephen J. Libertini, Yu Wang, Hsing-Jien Kung,Paramita Ghosh, and Maria Mudryj

2515� □S

Splicing of the Mycobacteriophage Bethlehem DnaB Intein.IDENTIFICATION OF A NEW MECHANISTIC CLASS OF INTEINS THATCONTAIN AN OBLIGATE BLOCK F NUCLEOPHILE. Kazuo Tori, BareketDassa, Margaret A. Johnson, Maurice W. Southworth, Lear E. Brace,Yoshizumi Ishino, Shmuel Pietrokovski, and Francine B. Perler

2610 Cordycepin Inhibits Protein Synthesis and Cell Adhesionthrough Effects on Signal Transduction. Ying Ying Wong, AliceMoon, Ruth Duffin, Adeline Barthet-Barateig, Hedda A. Meijer, MichaelJ. Clemens, and Cornelia H. de Moor

2807□S

Phosphorylation of Synucleins by Members of the Polo-likeKinase Family. Martial K. Mbefo, Katerina E. Paleologou, AhmedBoucharaba, Abid Oueslati, Heinrich Schell, Margot Fournier, DianaOlschewski, Guowei Yin, Markus Zweckstetter, Eliezer Masliah, PhilippJ. Kahle, Harald Hirling, and Hilal A. Lashuel

PROTEIN STRUCTURE AND FOLDING

2284 Analysis of Transmembrane Domains 1 and 4 of the HumanAngiotensin II AT1 Receptor by Cysteine-scanningMutagenesis. Liping Yan, Brian J. Holleran, Pierre Lavigne, EmanuelEscher, Gaetan Guillemette, and Richard Leduc

2415 Structure of a Eukaryotic Nonribosomal Peptide SynthetaseAdenylation Domain That Activates a Large HydroxamateAmino Acid in Siderophore Biosynthesis. T. Verne Lee, Linda J.Johnson, Richard D. Johnson, Albert Koulman, Geoffrey A. Lane,J. Shaun Lott, and Vickery L. Arcus

2537□S

The Binding Mode of ATP Revealed by the Solution Structure ofthe N-domain of Human ATP7A. Lucia Banci, Ivano Bertini,Francesca Cantini, Sayaka Inagaki, Manuele Migliardi,and Antonio Rosato

2580 Location of 3-Hydroxyproline Residues in Collagen Types I, II,III, and V/XI Implies a Role in Fibril Supramolecular Assembly.Mary Ann Weis, David M. Hudson, Lammy Kim, Melissa Scott, Jiann-JiuWu, and David R. Eyre

2686 Troponin Regulatory Function and Dynamics Revealed by H/DExchange-Mass Spectrometry. Devanand Kowlessurand Larry S. Tobacman

2857□S

The p53 Core Domain Is a Molten Globule at Low pH.FUNCTIONAL IMPLICATIONS OF A PARTIALLY UNFOLDED STRUCTURE.Ana Paula D. Ano Bom, Monica S. Freitas, Flavia S. Moreira, DaniellyFerraz, Daniel Sanches, Andre M. O. Gomes, Ana Paula Valente, YraimaCordeiro, and Jerson L. Silva

ENZYME CATALYSIS AND REGULATION

2302□S

IscS Functions as a Primary Sulfur-donating Enzyme byInteracting Specifically with MoeB and MoaD in theBiosynthesis of Molybdopterin in Escherichia coli. WanjiaoZhang, Alexander Urban, Hisaaki Mihara, Silke Leimkuhler, TatsuoKurihara, and Nobuyoshi Esaki

2721 Structural Insights into the Specificity of Xyn10B fromPaenibacillus barcinonensis and Its Improved Stability byForced Protein Evolution. Oscar Gallardo, F. I. Javier Pastor, JulioPolaina, Pilar Diaz, Robert Łysek, Pierre Vogel, Pablo Isorna, BeatrizGonzalez, and Julia Sanz-Aparicio

GLYCOBIOLOGY AND EXTRACELLULAR MATRICES

2232□S

N- and O-Glycans Modulate Galectin-1 Binding, CD45Signaling, and T Cell Death. Lesley A. Earl, Shuguang Bi,and Linda G. Baum

2386□S

Characterization of a New �(1–3)-Glucan Branching Activity ofAspergillus fumigatus. Amandine Gastebois, Isabelle Mouyna,Catherine Simenel, Cecile Clavaud, Bernadette Coddeville, MurielDelepierre, Jean-Paul Latge, and Thierry Fontaine

2456 The Endoplasmic Reticulum Chaperone Cosmc DirectlyPromotes in Vitro Folding of T-synthase. Rajindra P. Aryal,Tongzhong Ju, and Richard D. Cummings

LIPIDS AND LIPOPROTEINS: METABOLISM, REGULATION,AND SIGNALING

2796 Lipid Droplets Are Novel Sites of N-AcylethanolamineInactivation by Fatty Acid Amide Hydrolase-2. Martin Kaczocha,Sherrye T. Glaser, Janiper Chae, Deborah A. Brown,and Dale G. Deutsch

MEMBRANE TRANSPORT, STRUCTURE, FUNCTION,AND BIOGENESIS

2274□S

Prm1 Functions as a Disulfide-linked Complex in Yeast Mating.Valerie N. Olmo and Eric Grote

2294□S

Subunit Organization in the TatA Complex of the Twin ArginineProtein Translocase. A SITE-DIRECTED EPR SPIN LABELING STUDY.Gaye F. White, Sonya M. Schermann, Justin Bradley, Andrew Roberts,Nicholas P. Greene, Ben C. Berks, and Andrew J. Thomson

2554 Crystal Structures of Progressive Ca2� Binding States of theCa2� Sensor Ca2� Binding Domain 1 (CBD1) from the CALXNa�/Ca2� Exchanger Reveal Incremental ConformationalTransitions. Mousheng Wu, Hoa Dinh Le, Meitian Wang, VladimirYurkov, Alexander Omelchenko, Mark Hnatowich, Jay Nix, Larry V.Hryshko, and Lei Zheng

2569□S

The ClC-3 Cl�/H� Antiporter Becomes Uncoupled at LowExtracellular pH. James J. Matsuda, Mohammed S. Filali, Malia M.Collins, Kenneth A. Volk, and Fred S. Lamb

2653□S

Rhesus Glycoprotein P2 (Rhp2) Is a Novel Member of the RhFamily of Ammonia Transporters Highly Expressed in SharkKidney. Tsutomu Nakada, Connie M. Westhoff, Yoko Yamaguchi,Susumu Hyodo, Xiaojin Li, Takayuki Muro, Akira Kato, NobuhiroNakamura, and Shigehisa Hirose

2867□S

Identification of Transport-critical Residues in a FolateTransporter from the Folate-Biopterin Transporter (FBT)Family. Aymerick Eudes, Edmund R. S. Kunji, Alexandre Noiriel,Sebastian M. J. Klaus, Tim J. Vickers, Stephen M. Beverley, Jesse F.Gregory III, and Andrew D. Hanson

MOLECULAR BASIS OF CELL ANDDEVELOPMENTAL BIOLOGY

2326□S

The Neurospora Peptide:N-Glycanase Ortholog PNG1 IsEssential for Cell Polarity despite Its Lack of Enzymatic Activity.Sabine Maerz, Yoko Funakoshi, Yuki Negishi, Tadashi Suzuki,and Stephan Seiler

2428□S

Structure, Affinity, and Availability of Estrogen ReceptorComplexes in the Cellular Environment. Eric M. Kofoed, MartinGuerbadot, and Fred Schaufele

2463 The Cleavage of Semaphorin 3C Induced by ADAMTS1Promotes Cell Migration. Cary Esselens, Jordi Malapeira, NuriaColome, Carmen Casal, Juan Carlos Rodríguez-Manzaneque, FrancescCanals, and Joaquín Arribas

viii JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 285 • NUMBER 4 • JANUARY 22, 2010

2488 Dimerization of CPAP Orchestrates Centrosome CohesionPlasticity. Lingli Zhao, Changjiang Jin, Youjun Chu, Chris Varghese,Shasha Hua, Feng Yan, Yong Miao, Jing Liu, David Mann, Xia Ding,Jiancun Zhang, Zhiyong Wang, Zhen Dou, and Xuebiao Yao

2506 �-Amyloid Causes Depletion of Synaptic Vesicles Leading toNeurotransmission Failure. Jorge Parodi, Fernando J. Sepulveda,Jorge Roa, Carlos Opazo, Nibaldo C. Inestrosa, and Luis G. Aguayo

2527□S

The � Subunit of Voltage-gated Ca2� Channels Interacts withand Regulates the Activity of a Novel Isoform of Pax6. YunZhang, Yoichi Yamada, Mingming Fan, Saroja D. Bangaru, Bochao Lin,and Jian Yang

2562□S

The Full-length Unprocessed Hedgehog Protein Is an ActiveSignaling Molecule. Robert Tokhunts, Samer Singh, Tehyen Chu,Gisela D’Angelo, Valerie Baubet, John A. Goetz, Zhen Huang, ZiqiangYuan, Manuel Ascano, Yana Zavros, Pascal P. Therond, Sam Kunes,Nadia Dahmane, and David J. Robbins

2665□S

Modulation of SCF�-TrCP-dependent I�B� Ubiquitination byHydrogen Peroxide. Sami Banerjee, Jaroslaw W. Zmijewski,Emmanuel Lorne, Gang Liu, Yonggang Sha, and Edward Abraham

2707□S

Direct Observation of the Uncapping of Capping Protein-capped Actin Filaments by CARMIL Homology Domain 3. IkukoFujiwara, Kirsten Remmert, and John A. Hammer III

2734□S

Molecular Basis for Association of PIPKI�-p90 with ClathrinAdaptor AP-2. Nina Kahlfeldt, Ardeschir Vahedi-Faridi, Seong JooKoo, Johannes G. Schafer, Georg Krainer, Sandro Keller, WolframSaenger, Michael Krauss, and Volker Haucke

2750□S

In Vivo Nano-imaging of Membrane Dynamics in MetastaticTumor Cells Using Quantum Dots. Kohsuke Gonda, Tomonobu M.Watanabe, Noriaki Ohuchi, and Hideo Higuchi

2758 Jmjd1a Demethylase-regulated Histone Modification IsEssential for cAMP-response Element Modulator-regulatedGene Expression and Spermatogenesis. Zhaoliang Liu, SuolingZhou, Lan Liao, Xian Chen, Marvin Meistrich, and Jianming Xu

2847□S

Differential Regulation of Kainate Receptor Traffickingby Phosphorylation of Distinct Sites on GluR6. YukikoNasu-Nishimura, Howard Jaffe, John T. R. Isaac,and Katherine W. Roche

MECHANISMS OF SIGNAL TRANSDUCTION

2245□S

Cluster Analysis of Insulin Action in Adipocytes Reveals a KeyRole for Akt at the Plasma Membrane. Yvonne Ng, Georg Ramm,James G. Burchfield, Adelle C. F. Coster, Jacqueline Stockli,and David E. James

2309□S

BCAR3 Regulates Src/p130Cas Association, Src Kinase Activity,and Breast Cancer Adhesion Signaling. Natasha R. Schuh,Michael S. Guerrero, Randy S. Schrecengost, and Amy H. Bouton

2318 Inducible Priming Phosphorylation Promotes Ligand-independent Degradation of the IFNAR1 Chain of Type IInterferon Receptor. Sabyasachi Bhattacharya, Wei-ChunHuangFu, Jianghuai Liu, Sudhakar Veeranki, Darren P. Baker,Constantinos Koumenis, J. Alan Diehl, and Serge Y. Fuchs

2333□S

Transforming Growth Factor �-activated Kinase 1 (TAK1)Kinase Adaptor, TAK1-binding Protein 2, Plays Dual Roles inTAK1 Signaling by Recruiting Both an Activator and anInhibitor of TAK1 Kinase in Tumor Necrosis Factor SignalingPathway. Peter Broglie, Kunihiro Matsumoto, Shizuo Akira, David L.Brautigan, and Jun Ninomiya-Tsuji

2397□S

Reciprocal Negative Regulation of PDK1 and ASK1 Signaling byDirect Interaction and Phosphorylation. Hyun-A Seong, HaiyoungJung, Hidenori Ichijo, and Hyunjung Ha

2474□S

PGC-1� Serine 570 Phosphorylation and GCN5-mediatedAcetylation by Angiotensin II Drive Catalase Down-regulationand Vascular Hypertrophy. Shiqin Xiong, Gloria Salazar, AlejandraSan Martin, Mushtaq Ahmad, Nikolay Patrushev, Lula Hilenski, RafalRobert Nazarewicz, Minhui Ma, Masuko Ushio-Fukai,and R. Wayne Alexander

2498□S

Facilitating Akt Clearance via Manipulation of Hsp70 Activityand Levels. John Koren III, Umesh K. Jinwal, Ying Jin, John O’Leary,Jeff R. Jones, Amelia G. Johnson, Laura J. Blair, Jose F. Abisambra, LyraChang, Yoshinari Miyata, Anna M. Cheng, Jianping Guo, Jin Q. Cheng,Jason E. Gestwicki, and Chad A. Dickey

2591 Phosphorylation of �-Actinin 4 upon Epidermal Growth FactorExposure Regulates Its Interaction with Actin. Hanshuang Shao,Chuanyue Wu, and Alan Wells

2771□S

Constitutive Activity of the Human TRPML2 Channel InducesCell Degeneration. Shaya Lev, David A. Zeevi, Ayala Frumkin, VeredOffen-Glasner, Gideon Bach, and Baruch Minke

2783□S

Soluble Nogo Receptor Down-regulates Expression ofNeuronal Nogo-A to Enhance Axonal Regeneration. XiangminPeng, Zhigang Zhou, Jian Hu, David J. Fink, and Marina Mata

2823 Role of Interfacial Water Molecules in Proline-rich LigandRecognition by the Src Homology 3 Domain of Abl. AndresPalencia, Ana Camara-Artigas, M. Teresa Pisabarro, Jose C. Martinez,and Irene Luque

2834□S

Nuclear Isoforms of Fibroblast Growth Factor 2 Are NovelInducers of Hypophosphatemia via Modulation of FGF23 andKLOTHO. Liping Xiao, Takahiro Naganawa, Joseph Lorenzo, ThomasO. Carpenter, J. Douglas Coffin, and Marja M. Hurley

2886□S

Phosphoinositide 3-Kinase p110� Regulates Integrin �IIb�3

Avidity and the Cellular Transmission of Contractile Forces.Simone M. Schoenwaelder, Akiko Ono, Warwick S. Nesbitt, Joanna Lim,Kate Jarman, and Shaun P. Jackson

JANUARY 22, 2010 • VOLUME 285 • NUMBER 4 JOURNAL OF BIOLOGICAL CHEMISTRY ix