33
Ver 2001 (c) 2001, Purdue University 1 On the Web at: http://www.agry.purdue.edu/ext/pdf/Transgenic_Crops-2001.pdf Transgenic Crops in Indiana Bob Nielsen Purdue University Email: [email protected] Web: http://www.kingcorn.org

Transgenic Crops in Indiana - Purdue University Transgenic Crops in Indiana Bob Nielsen Purdue University Email: [email protected] Web: Genetic Crop Improvement Ver 2001 (c) 2001,

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Ver

2001

(c)

2001

, Pur

due

Uni

vers

ity1

On

the

Web

at:

http

://w

ww

.agr

y.pu

rdue

.edu

/ext

/pdf

/Tra

nsge

nic_

Cro

ps-2

001.

pdf

Tran

sgen

ic C

rops

in I

ndia

na

Bob

Nie

lsen

Purd

ue U

nive

rsity

Emai

l: rn

iels

en@

purd

ue.e

duW

eb:

http

://w

ww

.kin

gcor

n.or

g

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity2

Gen

etic

Cro

p Im

prov

emen

tTh

e ‘o

ld-f

ashi

oned

’ way

to

achi

eve

gene

tic

impr

ovem

ent

in c

orn

and

soy

was

thr

ough

tr

aditi

onal

sex

ual a

cts

amon

g co

nsen

ting,

or

som

etim

es n

ot c

onse

ntin

g, p

lant

s of

the

sam

e sp

ecie

s.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity3

‘Nat

ural

’ Cor

n Pl

ant

Sex

Polle

n pr

oduc

ed in

th

e ta

ssel

ant

hers

co

ntai

ns t

he m

ale

gene

tic m

ater

ial.

Gra

vity

, win

d or

hum

an

inte

rven

tion

allo

ws

the

polle

n to

fer

tiliz

e th

e ov

ules

.

This

‘nat

ural

’ sex

has

be

en g

oing

on

for

thou

sand

s of

yea

rs!

Ovu

les

prod

uced

on

the

ears

con

tain

the

fem

ale

gene

tic m

ater

ial.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity4

Res

ults

of

‘Nat

ural

’ Cro

p Se

x

Dom

estic

atio

n of

cro

ps.

Impr

oved

cro

p tr

aits

for

en

hanc

ed y

ield

and

/or

grai

n qu

ality

.G

enet

ic m

odifi

catio

n (G

M)

of c

rop

spec

ies

by:

Rea

rran

gem

ent

of g

enes

with

in a

spe

cies

via

na

tura

l or

dire

cted

-pol

linat

ion

Inco

rpor

atio

n of

gen

es f

rom

“w

ild”

or “

clos

e”

rela

tives

via

nat

ural

cro

ss-p

ollin

atio

nIm

age

Sour

ce:

USD

A, I

mag

e N

umbe

r K7

743-

13ht

tp:/

/ww

w.a

rs.u

sda.

gov/

is/g

raph

ics/

phot

os/

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity5

‘Nat

ural

’ GM

of

Crop

s W

orks

!

Indi

ana

corn

gra

in y

ield

s ha

ve in

crea

sed

by 1

.6

bush

els

per

acre

per

yea

r fo

r th

e la

st 7

0 ye

ars.

Pr

imar

ily d

ue t

o ‘n

atur

al’

gene

tic m

odifi

catio

n (G

M)

and

impr

oved

cro

p pr

oduc

tion

tech

nolo

gies

.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity6

But,

som

e w

orry

…Th

e an

nual

yie

ld g

ain

(1.6

bu/

ac/y

r) a

s a

perc

ent

of

the

yiel

d po

tent

ial o

f th

e co

rn c

rop

has

been

de

crea

sing

for

the

pas

t 70

yea

rs.

Nag

ging

Que

stio

n:Ca

n w

e ke

ep u

p w

ith

the

incr

easi

ng g

loba

l ne

ed f

or f

ood?

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity7

Lim

its t

o Cr

op B

reed

ing?

Slow

pro

cess

to

deve

lop

new

var

ietie

s.N

ot u

ncom

mon

for

8 –

10 y

ears

fro

m s

tart

to

finis

h us

ing

trad

ition

al b

reed

ing

tech

niqu

es.

Des

ired

new

or

impr

oved

tra

its n

ot a

lway

s pr

esen

t in

cro

p sp

ecie

s.D

isea

se o

r in

sect

res

ista

nce

Phys

iolo

gica

l im

prov

emen

tsSt

ress

tol

eran

ceG

rain

or

plan

t co

mpo

sitio

n

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity8

Thus

, Gen

etic

Eng

inee

ring

Allo

ws

for

fast

er v

arie

ty d

evel

opm

ent.

Tiss

ue c

ultu

re, r

ecom

bina

nt D

NA,

etc

.

Allo

ws

for

tran

sfer

ring

here

tofo

re

unav

aila

ble

gene

tic t

raits

into

a c

rop

varie

ty f

rom

unr

elat

edsp

ecie

s.e.

g., t

rans

geni

c tr

aits

suc

h as

Bt

inse

ct

resi

stan

ce o

rigin

atin

g fr

om s

oil b

acte

ria.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity9

Tran

sgen

ic E

xam

ple:

Bt

Bt g

enes

orig

inat

e fr

om t

he s

oil b

acte

ria, B

acill

us

thur

ingi

ensis

Res

earc

hers

can

iden

tify

and

isol

ate

the

gene

s.“B

last

ed”

into

cor

n D

NA

usin

g go

ld p

artic

le g

ene

guns

Bt g

enes

tha

t su

cces

sful

ly

inco

rpor

ate

into

cor

n D

NA

expr

ess

the

inse

ctic

idal

Bt

prot

ein

in c

orn

plan

t gr

own

by f

arm

er.

Imag

e So

urce

: U

niv.

of

Neb

rask

aht

tp:/

/ww

w.a

gbio

safe

ty.u

nl.e

du/e

duca

tion/

sum

mar

y.ht

m

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity10

Gen

es v

s. E

vent

sD

iffer

ent

vers

ions

of

a ge

ne m

ay e

xist

.Cr

y1a(

b), C

ry1a

(c),

Cry

9c, e

tc. f

or B

t ge

nes.

Thus

, diff

eren

t va

rietie

s m

arke

ted.

The

com

bina

tion

of e

xact

gen

e ve

rsio

n an

d its

ev

entu

al lo

catio

n w

ithin

the

cor

n ge

nom

e is

te

rmed

an

‘eve

nt’.

Two

com

pani

es m

ay u

se t

he s

ame

Bt g

ene,

but

loca

ted

in d

iffer

ent

DN

A po

sitio

ns;

thus

tw

o di

ffer

ent

‘eve

nts’

.

Imag

e So

urce

: U

niv.

of

Neb

rask

aht

tp:/

/ww

w.a

gbio

safe

ty.u

nl.e

du/e

duca

tion/

tran

sfor

mat

ion.

htm

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity11

So, w

hat

abou

t St

arLi

nk™

? St

arLi

nk™

cor

n hy

brid

s co

ntai

ned

the

Cry9

C in

sect

icid

al g

ene

from

Bac

illus

th

urin

gien

sis

Tech

nica

lly, i

s a

diff

eren

t ve

rsio

n of

the

Bt

gene

, but

has

the

sam

e m

ode

of a

ctio

n as

ot

her

Bt g

enes

How

ever

, the

pro

tein

has

diff

eren

t bi

ndin

g si

te

in t

he in

sect

gut

tha

n th

at f

rom

oth

er B

t ve

rsio

ns, w

hich

mad

e it

attr

activ

e fo

r co

mba

ting

inse

ct r

esis

tanc

e to

Bt.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity12

Com

mon

con

sum

er c

once

rns?

Fear

s of

uni

dent

ified

foo

d sa

fety

ris

ksfr

om t

rans

geni

c m

odifi

catio

nsTo

xins

Carc

inog

ens

Die

tary

pre

fere

nces

Not

a r

isk,

but

a m

atte

r of

cho

ice

Alle

rgen

sSo

ybea

n &

Bra

zil n

ut g

enes

Kraf

t™ t

aco

shel

l rec

all &

Sta

rLin

k™ B

t co

rn

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity13

So, w

hat

abou

t St

arLi

nk™

? St

abili

ty o

f pr

otei

n to

hea

t an

d ga

stric

di

gest

ion

are

grea

ter

than

tho

se o

f ot

her

Bt g

enes

Was

the

rea

son

for

alle

rgen

unc

erta

inty

on

the

beha

lf of

EPA

reg

ulat

ors

Was

the

rea

son

for

non-

appr

oval

of

Star

Link

™ c

orn

for

hum

an f

ood

use.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity14

Com

mon

con

sum

er c

once

rns?

Fear

s of

uni

dent

ified

env

ironm

enta

l ris

ksfr

om

tran

sgen

ic m

odifi

catio

nsO

ff-t

arge

t in

jury

or

deat

h to

non

pest

sM

onar

ch b

utte

rflie

s &

Bt

corn

pol

len

Gen

etic

sel

ectio

n pr

essu

re f

or r

esis

tanc

e to

tr

ansg

enic

tra

it or

her

bici

de u

sed

with

tra

nsge

nic

trai

t Gly

phos

ate

(Rou

ndup

™)

& r

yegr

ass

in A

ustr

alia

Gen

e es

cape

s (c

ross

-pol

linat

ion)

to

wee

dy r

elat

ives

of

cro

psCa

nola

(ra

pese

ed)

& w

eedy

mus

tard

s in

Can

ada

Corn

& w

ild r

elat

ives

in M

exic

o

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity15

Com

mon

con

sum

er c

once

rns?

Fear

s of

cor

pora

te

mon

opol

ies

in

agric

ultu

ral p

rodu

ctio

nCo

ntro

l of

glob

al f

ood

supp

lyEl

imin

atio

n of

sm

all

farm

ers

Top

7 A

groc

hem

ical

Co'

s

5.9

3.9

3.7

2.5

2.3

2.3

2.2

Syng

enta Mon

sant

o Avent

is DuPon

tDowBa

yer

BASF

20

00

sa

les

($ b

illi

on

)

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity16

Mar

ketp

lace

res

pons

e?G

rain

impo

rter

s, b

uyer

s &

pro

cess

ors

have

re

spon

ded

to c

onsu

mer

con

cern

s by

lim

iting

w

hich

tra

nsge

nic

varie

ties

they

will

pur

chas

e.Eu

rope

an U

nion

has

an

acce

pted

list

Incl

udes

Rou

ndup

Rea

dy®

soy

& m

ost

Bt c

orn,

but

not

Rou

ndup

Rea

dy®

cor

n or

Sta

rLin

k™ B

t co

rn

Acce

ptan

ce b

y pr

oces

sors

& p

ublic

is le

ss c

erta

in

for

the

imm

edia

te f

utur

eFr

ito-L

ay, G

erbe

r, S

tale

y, M

cDon

alds

, Nat

iona

l Sta

rch

Kraf

t (t

aco

shel

l rec

all)

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity17

Tran

sgen

ic C

rops

in I

ndia

naH

erbi

cide

tol

eran

t va

rietie

sG

lyph

osat

e re

sist

ant

soy

& c

orn

Rou

ndup

Rea

dy®

Glu

fosi

nate

-am

mon

ium

res

ista

nt c

orn

Libe

rty

Link

®

Inse

ct r

esis

tant

var

ietie

sBt

cor

n Pr

imar

ily E

urop

ean

corn

bor

er c

ontr

olSe

vera

l Bt

gene

s an

d ev

ents

mar

kete

d

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity18

Tran

sgen

ic C

orn

in U

.S.

6%6%

0%5%10%

15%

20%

25%

30%

35%

40%

NE

IAIL

INO

HU

.S.

Percent of corn acres

Stac

ked

HT

Bt

2001

Cro

p Y

ear

Sour

ce: U

SDA

-NA

SS, J

une

2001

, on

the

Web

at:

http

://us

da.m

annl

ib.c

orne

ll.ed

u/re

ports

/nas

sr/fi

eld/

pcp-

bba/

acrg

0601

.txt

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity19

Tran

sgen

ic S

oy in

U.S

.

78%0%10

%20

%30

%40

%50

%60

%70

%80

%90

%

NE

IAIL

INO

HU

.S.

Percent of soy acres

HT

2001

Cro

p Y

ear

Sour

ce: U

SDA

-NA

SS, J

une

2001

, on

the

Web

at:

http

://us

da.m

annl

ib.c

orne

ll.ed

u/re

ports

/nas

sr/fi

eld/

pcp-

bba/

acrg

0601

.txt

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity20

Issu

es f

or I

ndia

na F

arm

ers

Seed

cos

t of

TG

cro

ps?

Typi

cally

mor

e ex

pens

ive

than

non

-TG

sAg

rono

mic

ben

efits

of

TG c

rops

?So

met

imes

que

stio

nabl

eAg

rono

mic

dis

adva

ntag

es o

f TG

cro

ps?

Som

etim

es ig

nore

dM

arke

t un

cert

aint

ies

of T

G c

rops

?So

met

imes

dra

mat

icTG

con

sequ

ence

for

non

-TG

cro

ps?

Can

be c

ostly

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity21

Gro

w T

G c

rops

or

not?

Mus

t de

term

ine

the

bala

nce

betw

een

agro

nom

ic c

osts

, agr

onom

ic o

r ec

onom

ic

bene

fits

and

mar

ket

unce

rtai

ntie

s

For

exam

ple:

Hig

h co

st +

litt

le b

enef

it +

unc

erta

in m

arke

t =

su

bsta

nti

al e

con

omic

ris

k

Hig

h co

st +

som

e be

nefit

+ f

eed

own

lives

tock

=

littl

e ec

onom

ic r

isk

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity22

TG c

rops

as

agro

nom

ic in

puts

Nie

lsen

’s O

pini

on:

Inse

ct-r

esis

tant

or

herb

icid

e-to

lera

nt v

arie

ties

are

NO

T CR

ITIC

AL f

or t

he

succ

essf

ul p

rodu

ctio

n of

co

rn &

soy

bean

in I

ndia

na!

In o

ther

wor

ds, g

row

ing

non-

TGs

will

not

res

ult

in e

cono

mic

rui

n fo

r m

ost

Indi

ana

farm

ers!

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity23

Exam

ple:

Bt

corn

An “

insu

ranc

e” t

rait

The

Bt g

ene

or t

rait,

by

itsel

f, d

oes

not

incr

ease

yie

ldO

nly

usef

ul w

hen

pest

occ

urs

Litt

le p

ayba

ck in

yea

rs w

hen

Euro

pean

cor

n bo

rer

(ECB

) in

fest

atio

ns a

re m

inor

For

exam

ple,

199

8 &

199

9 in

Ind

iana

ECB

dam

age

in 2

000

and

2001

mor

e se

vere

, bu

t da

mag

e st

ill q

uest

iona

ble

econ

omic

ally

.Im

age

Sour

ce:

USD

A, I

mag

e N

umbe

r K2

634-

8ht

tp:/

/ww

w.a

rs.u

sda.

gov/

is/g

raph

ics/

phot

os/

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity24

Our

pos

ition

on

Bt c

orn

…Bt

cor

n is

not

eco

nom

ical

for

the

ave

rage

In

dian

a co

rn f

ield

due

to

low

his

toric

al

freq

uenc

ies

and

seve

ritie

s of

ECB

out

brea

ks

rela

tive

to “

tech

fee

s” o

f hy

brid

s (P

urdu

e Ex

tens

ion

Publ

icat

ion

ID-2

19)

25%

12%

6%7%

24%

NE

IAIL

INO

HM

ore

<<

<<

<<

< E

CB

Pre

ssur

e >

>>

>>

>>

Les

s

Bt

acre

age,

200

1

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity25

So, w

hat

abou

t RR s

oy?

Why

so

popu

lar?

Goo

d w

eed

cont

rol

Low

er h

erbi

cide

cos

tFo

r m

any

farm

ers

Mor

e tim

e fle

xibi

lity

Less

cro

p in

jury

Less

mgm

t. e

ffor

t

Any

disa

dvan

tage

s?H

ighe

r se

ed c

ost

Appl

’n t

imin

gSo

me

wai

t to

o lo

ng t

o ap

ply

herb

icid

e an

d co

nseq

uent

ly s

uffe

r yi

eld

loss

to

early

w

eed

pres

sure

Mar

ket

acce

ptan

ce?

Som

etim

es a

n is

sue

Gen

etic

yie

ld d

iff.?

76%

73%

64%

78%

64%

NE

IAIL

INO

H

RR

so

y ac

reag

e, 2

00

1

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity26

TG C

onse

quen

ces

for

non-

TGBe

caus

e so

me

grai

n bu

yers

will

not

ac

cept

TG

gra

in, s

ome

farm

ers

need

to

take

ext

ra p

reca

utio

ns t

o av

oid

TG

cont

amin

atio

n of

the

ir no

n-TG

gra

in.

TRA

NSL

ATI

ON

:Ex

tra

cost

s an

d he

adac

hes

plac

ed o

n fa

rmer

s an

d th

e gr

ain

buyi

ng in

dust

ry.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity27

Cost

s of

non

-TG

pro

duct

ion

Ensu

ring

seed

ing

purit

ySe

ed s

ourc

e ce

rtifi

catio

n fo

r no

n-TG

Prio

r cr

op in

the

fie

ldPl

antin

g op

erat

ion

Avoi

ding

cro

ss-p

ollin

atio

n w

/ TG

s (c

orn)

TGs

or n

ot in

adj

acen

t fie

lds

Buff

er r

ows

arou

nd e

dges

of

field

Tink

erin

g w

/ pl

antin

g da

te t

o m

anip

ulat

e tim

ing

of

polli

natio

n of

cor

n re

lativ

e to

adj

. fie

lds

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity28

Cost

s of

non

-TG

pro

duct

ion

Ensu

ring

harv

estin

g pu

rity

Supe

r cl

eano

ut o

f ha

rves

ter

prio

r to

har

vest

of

non-

TG f

ield

Har

vest

non

-TG

fie

lds

befo

re T

G f

ield

sAv

oidi

ng g

rain

com

min

glin

gSu

per

clea

nout

of

stor

age

faci

litie

sSt

ore

TG s

epar

ate

from

non

-TG

Supe

r cl

eano

ut o

f tr

ansp

ort

equi

pmen

tCo

nsid

er s

ellin

g or

del

iver

ing

grai

n di

rect

ly f

rom

fie

ld h

arve

st

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity29

Thou

ghts

cou

rtes

y of

:

Dirk

Mai

er, P

urdu

e Ag

. Eng

r.

Thou

ghts

cou

rtes

y of

:

Dirk

Mai

er, P

urdu

e Ag

. Eng

r.

Star

Link

™ le

sson

s fo

r fu

ture

Biot

ech

firm

s sh

ould

not

be

allo

wed

to

mar

ket

a bi

otec

h va

riety

unt

il:Th

e va

riety

has

bee

n ap

prov

ed b

y re

gula

tory

ag

enci

es a

nd a

ccep

ted

by t

he g

rain

indu

stry

for

all

end

use

s, in

clud

ing

feed

, ind

ustr

ial,

food

and

ph

arm

aceu

tical

;

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity30

Star

Link

™ le

sson

s fo

r fu

ture

Biot

ech

firm

s sh

ould

not

be

allo

wed

to

mar

ket

a bi

otec

h va

riety

unt

il:Th

e va

riety

has

bee

n ap

prov

ed b

y re

gula

tory

age

ncie

s an

d ac

cept

ed b

y th

e gr

ain

indu

stry

for

all

end

uses

, inc

ludi

ng f

eed,

indu

stria

l, fo

od a

nd p

harm

aceu

tical

;

Rel

iabl

e an

d in

expe

nsiv

e te

stin

g te

chn

olog

yis

av

aila

ble

for

use

at t

he f

irst

poin

t of

sal

e (s

uch

as

strip

-tes

t ki

ts f

or e

leva

tor

use)

;

Cas

e in

Poi

nt:

Rou

ndup

Rea

dy®

cor

n, 2

001

harv

est s

easo

n, te

st k

its a

vaila

ble

(as

of 1

0/8/

01) f

or n

ew N

K603

eve

nt (l

esse

r acr

eage

) but

not

old

er

GA2

1 ev

ent (

grea

ter a

crea

ge).

Cas

e in

Poi

nt:

Rou

ndup

Rea

dy®

cor

n, 2

001

harv

est s

easo

n, te

st k

its a

vaila

ble

(as

of 1

0/8/

01) f

or n

ew N

K603

eve

nt (l

esse

r acr

eage

) but

not

old

er

GA2

1 ev

ent (

grea

ter a

crea

ge).

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity31

Star

Link

™ le

sson

s fo

r fu

ture

Biot

ech

firm

s sh

ould

not

be

allo

wed

to

mar

ket

a bi

otec

h va

riety

unt

il:Th

e va

riety

has

bee

n ap

prov

ed b

y re

gula

tory

age

ncie

s an

d ac

cept

ed b

y th

e gr

ain

indu

stry

for

all

end

uses

, inc

ludi

ng f

eed,

indu

stria

l, fo

od a

nd

phar

mac

eutic

al;

Relia

ble

and

inex

pens

ive

test

ing

tech

nolo

gy is

ava

ilabl

e fo

r us

eat

the

firs

t po

int

of s

ale

(suc

h as

str

ip-t

est

kits

for

ele

vato

r us

e);

and

Tole

ran

ce le

vels

base

d on

det

ecta

ble

limits

hav

e be

en e

stab

lishe

d in

ord

er t

o gi

ve t

he U

.S. g

rain

ha

ndlin

g, e

xpor

ting

and

proc

essi

ng in

dust

ry t

he

abili

ty t

o m

eet

cust

omer

dem

ands

wit

h r

espe

ct

to t

he

pres

ence

of

appr

oved

bu

t u

nde

sire

d tr

ansg

enic

cro

p tr

aits

in t

heir

end-

prod

ucts

.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity32

Bott

om L

ine

The

“GM

O”

deba

te w

ill c

ontin

ue in

to t

he n

ear

futu

re.

Curr

ent

TGs

are

not

criti

cal f

or t

he a

gron

omic

su

cces

s of

mos

t In

dian

a co

rn/s

oy o

pera

tions

.Fa

rmer

’s c

hoic

e to

gro

w o

r no

t de

pend

s pr

imar

ily o

n hi

s/he

r pe

rcep

tion

of t

he

agro

nom

ic b

enef

its o

f th

e TG

, the

mar

ket

unce

rtai

ntie

s fo

r TG

s in

the

com

ing

crop

yea

r &

see

d av

aila

bilit

y of

non

-TG

var

ietie

s.

Ver

2001

(c)

2001

, Pu

rdue

Uni

vers

ity33

Refe

renc

esH

yde,

J.,

M.A

. Mar

tin, P

.V. P

reck

el, C

. R. E

dwar

ds. 1

998.

The

Eco

nom

ics

of B

t Co

rn:

Impl

icat

ions

for

Ado

ptio

n. P

urdu

e Ex

t. p

ub I

D-2

19. O

n th

e W

eb a

t: h

ttp:

//w

ww

.agc

om.p

urdu

e.ed

u/Ag

Com

/Pub

s/ID

/ID

-219

.pdf

Nie

lsen

, Bob

. 200

1. B

iote

chno

logy

Iss

ues

on t

he W

eb. O

n th

e W

eb a

t:

http

://w

ww

.kin

gcor

n.or

g/ca

fe/b

iote

ch.h

tml

Nie

lsen

, Bob

. 200

1. S

ilk E

mer

genc

e. O

n th

e W

eb a

t:

http

://w

ww

.kin

gcor

n.or

g/ne

ws/

artic

les.

01/S

ilks-

0626

.htm

lN

iels

en, B

ob. 2

001.

Tas

sel E

mer

genc

e &

Pol

len

Shed

. On

the

Web

at:

http

://w

ww

.kin

gcor

n.or

g/ne

ws/

artic

les.

01/T

asse

ls-0

626.

htm

lN

iels

en, B

ob a

nd D

irk M

aier

. 200

1. G

MO

Iss

ues

Faci

ng I

ndia

na F

arm

ers

in 2

001.

On

the

Web

at:

ht

tp:/

/ww

w.a

gcom

.pur

due.

edu/

AgCo

m/P

ubs/

GQ

/GQ

-46.

pdf

Uni

v. o

f N

ebra

ska-

Linc

oln.

200

1. A

gBio

safe

ty. O

n th

e W

eb a

t:

http

://w

ww

.agb

iosa

fety

.unl

.edu

/