updated on 31.01.2018 NETWORK OF OFFICIAL …...updated on 31.01.2018 a.1 (54-104) a.2 (107-149) a.3 (152-249) a.4 (252-302) a.5 dvca/department of food hygiene (305-356) a.6 (359-403)

updated on 31.01.2018

A.1 (54-104)

A.2 (107-149)

A.3 (152-249)

A.4 (252-302)

A.5 DVCA/DEPARTMENT OF FOOD HYGIENE (305-356)

A.6 (359-403)

A.7 (406-450)

A.8 (453-505)

A.9 (508-544)

A.10 (547-599)

A.11 (602-641)

A.12 (644-685)

DIRECTORATE OF VETERINARY CENTER OF THESSALONIKI (DVCTh)

B.1 DVCTh/DEPARTMENT OF VIRAL, RICKETTSIAL DISEASES AND TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (691-719)

B.2 DVCTh/DEPARTMENT OF FOOD MICROBIOLOGY, BIOCHEMICAL & PHYSICOCHEMICAL FOOD CONTROL, RESIDUES AND MARINE BIOTOXINS & TOXINS IN OTHER WATERS (722-755)

B.3 DVCTh/DEPARTMENT OF AVIAN & BEE DISEASES AND AQUATIC ORGANISMS' PATHOLOGY (758-815)

B.4 DVCTh/DEPARTMENT OF MICROBIOLOGY, INFECTIOUS DISEASES & BRUCELLOSIS (818-855)

B.5 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF IOANNINA (858-902)

B.6 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF KAVALA (905-955)

B.7 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF SERRES (958-1023)

B.8 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF LARISSA (1026-1079)

B.9 DVCTh/BIOLOGICAL PRODUCTS, DIAGNOSTIC PATHOLOGY & PARASITOLOGY OFFICE (1082-1120)

DIRECTORATE OF VETERINARY CENTER OF ATHENS (DVCA)

DVCA/DEPARTMENT OF DIAGNOSTIC PATHOLOGY, ANATOMY, HISTOLOGY & MICROBIOLOGY

DVCA/DEPARTMENT OF PARASITOLOGY-PARASITIC DISEASES, ENTOMOLOGY & BEE HEALTH

DVCA/DEPARTMENT OF MOLECULAR DIAGNOSTICS, FOOT & MOUTH DISEASE, VIRAL, RICKETTSIAL AND EXOTIC DISEASES

DVCA/DEPARTMENT OF PATHOLOGY OF AQUATIC ORGANISMS

DVCA/DEPARTMENT OF TOXICOLOGY, RESIDUES & ENVIRONMENTAL CONTAMINANTS

DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHALKIS

DVCA/DEPARTMENT: VETERINARY LABORATORY OF TRIPOLIS

DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHANIA

DVCA/DEPARTMENT: VETERINARY LABORATORY OF HERACLION

DVCA/DEPARTMENT: VETERINARY LABORATORY OF PATRAS

DVCA/DEPARTMENT: VETERINARY LABORATORY OF RHODES

NETWORK OF OFFICIAL LABORATORIES OF THE GENERAL DIRECTORATE OF VETERINARY SERVICES, MINISTRY OF RURAL DEVELOPMENT & FOOD

Σελίδα 1 από 50

54 A.1 DVCA/DEPARTMENT OF DIAGNOSTIC PATHOLOGY, ANATOMY, HISTOLOGY & MICROBIOLOGY55

56 25, Neapoleos Street, 153 10 Agia Paraskevi57 tel:+30 210 6399521, fax:+30 210 6399521

58 [email protected], [email protected]

60 NATIONAL REFERENCE LABORATORY

61 #62

63 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:

64 #

65 #

66

67 ACCREDITED TESTS PERFORMED68

69 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until

70 1Blood serum originating from

ruminants

Detection of mycobacterium avium spp.

paratuberculosis antibodies

ELISA ΚΙΤ POURQUIER / IDEXX

(D07130-5)Directive64/432 ESYD 695-2 15/7/2018

71 2Blood serum samples or

plasma from ruminants

Detection of antibodies against Coxiella

burnetii (Q -fever)Indirect ELISA

ELISA Test according to the

O.I.E. Manual of Diagnostic

Tests and Vaccines for

Terrestrial Animals

(Chapter 2.1.16. -2015)

ESYD 695-2 15/7/2018

72 3

Blood serum samples of

domestic or wild ruminants,

equine, swine, camelids,

carnivores and human origin

Detection of antibodies against Βrucella

abortus/ melitensis/ suis

Rose Bengal Test (R.B.) according to the

Commission Regulation 535/2002/EC

amending Annex C of the Directive

64/432/EEC and the Decision 2000/330/EC

Directive64/432 ESYD 695-2 15/7/2018

73 4







Complement Fixation Test (C.F.T.) according

to the Commission Regulation 535/2002/EC



Directive64/432 ESYD 695-2 15/7/2018

74 5Milk or blood serum samples

of domestic or wild ruminants


abortus/ melitensis/ suisIndirect ELISA

ELISA Test according to the

Commission Regulation

535/2002/EC amending

Annex C of the Directive

64/432/EEC and the

ESYD 695-2 15/7/2018

75

DIRECTORATE OF VETERINARY CENTER OF ATHENS (DVCA)

Legal basis: Directive 64/432/EEC, Presidential Decree 41/2006(Α'44), Ministerial Decision 30/3430/14.01.2015(B'171)

Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)

Zoonoses & zoonotic agents (Bovine Tuberculosis)

Zoonoses & zoonotic agents (Brucelloses)

Zoonoses & zoonotic agents (Samonella)Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &

1751/51822/24.04.2013(B'1141)


tel:+30 210 6399521, fax:+30 210 6399521

mailto:[email protected]

76 NON-ACCREDITED TESTS PERFORMED77

78 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments

79 6Lymph nodes - Internal

organs (Lungs, spleen, liver ) Bovine tuberculosis

Interferon assay, tuberculin test, culture,

direct microscopy, PCR. PendingDirective 64/432

80 7Sections of small/large

intestine & mesenteric lymph

nodes - Faeces

Detection of Mycobacterium paratuberculosis Staining, microscopy OIE Terrestrial

Manual,2017 Chapter

2.1.15

81 8 Bovine Blood serum, lungs

Detection of Contagious bovine

pleuropneumonia mycoplasma (Mycoplasma

mycoides subsp. mycoides SC )

ΕLISA, Culture, PCR (upon availability of

reagents)



Terrestrial Animals

(Chapter 2.4.8. -2014).

82 9Sheep and Goat Blood serum,

milk

Detection of antibodies against Mycoplasma

agalactiaeELISA, Culture, PCR



Terrestrial Animals

(Chapter 2.7.4. -2013).

83 10 Lung tissue Detection of Mycoplasma spp Culture, stereoscopy, isolation, identification

84 11Lungs, synovial fluid , ocular

secretion

Detection of Contagious caprine

pleuropneumonia mycoplasma ( Mycoplasma

capricolum subsp.Capripneumoniae)

ΕLISA, Culture, PCR (upon availability of

reagents)



Terrestrial Animals

(Chapter 2.7.5. -2014).

85 12 Internal organsDetection of Escherichia coli, Clostridium

perfringensCulture, isolation, identification

86 13 Lung tissueDetection of Pasteurella

multocida/Mannheimia haemolyticaCulture, isolation, identification

87 14 Internal organsDetection of Staphylococcus aureus &

coagulase negative staphylococciCulture, isolation, identification

88 15 Lung tissueDetection of Pasteurella multocida /

Mannheimia haemolyticaCulture, isolation, identification


89 16 Internal organs Detection of Streptococcus spp Culture, isolation, identification

90 17Internal organs, joints, lungs

from pigs, rabbits and

ruminants

Detection of Haemophilus spp, Βordetella spp,

Erysipelothrix spp, Culture, microscopy, isolation, identification

91 18 Internal organs Detection of Pseudomonas spp Culture, microscopy, isolation, identification

92 19 Cultures AntibiogramsAntimicrobial Susceptibility–Agar Disk

Diffusion Test

Test according to the valid

editions of CLSI:Μ2,

CLSI:M100, Eucast93 20 Internal organs Detection of moulds Culture, staining

94 21 Milk and udder secretionsDetection of Escherichia coli, Klebsiella spp,

Enterobacter spp and other

enterobacteriaceae

Culture, microscopy, biochemical testing

95 22 Milk and udder secretionsDetection of Staphylococcus aureus &

coagulase negative staphylococci Culture, microscopy, biochemical testing

96 23 Milk and udder secretionsDetection of Streptococcus agalactiae,

Streptococcus uberis, Streptococcus

dysgalactiae


97 24 Milk and udder secretions

Detection of Enterococcus spp, Proteus spp,

Pasteurella spp, Pseudomonas spp,

Mycoplasma spp, Corynebacterium spp,

Trueperella pyogenes


98 25 Milk and udder secretions Detection of yeasts Culture, microscopy, biochemical testing

99 26 Blood serum Detection of Salmonella Pullorum Culture, isolation, rapid agglutination

100 27 Feces , Blood serumDetection of antibodies against avian

mycoplasmosis (M. Gallisepticum )ELISA, rapid agglutination

101 28 Blood serumDetection of antibodies against avian

mycoplasmosis (M. synoviae ) ELISA, rapid agglutination

102 29 Yoghurt , egg shells Detection of Salmonella spp. in poultry ISO 6579:2002 ISO 6579:2002

103 30 Trachea - Lungs Detection of Mycoplasma spp in poultry Culture, microscopy

104 31 Internal organs and feces Detection of fungi in poultryCulture ( enriched substrates), microscopy,

staining105


106

107 A.2 DVCA/DEPARTMENT OF PARASITOLOGY-PARASITIC DISEASES, ENTOMOLOGY & BEE HEALTH108

109 25, Neapoleos Street, 153 10 Agia Paraskevi110 tel:+30 210 6002217, fax:+30 210 6002217111 [email protected]


114 #

115

116 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:117 #

118 #

119



123 1 Pork meat Detection of larvae of parasite Trichinella spp Magnetic stirrer method for pooled-sample

digestion (EURL Protocol) Reg2015/1375 ESYD 695-2 15/7/2018

124 2 Dog blood serum Detection of anti-Leishmania antibodies

Indirect immunofluorescence antibody test

IFAT (IN HOUSE) according to the OIE

Terrestrial Manual (2014) Leishmaniosis

(Chapter 2.1.11)

In house method - O.I.E.

Terrestrial Manual (2014)

Leishmaniosis (Chapter

2.1.11)

ESYD 695-2 15/7/2018

125 3 Fish tissue Detection of the parasite Anisakis spp Magnetic stirrer method for pooled-sample

digestion according to EURL Protocol

Protocol according to

European Union Reference

Laboratory for parasites

(SOPs)

ESYD 695-2 15/7/2018

126



130 4Blood serum samples of sheep,

goats, pigs, dogs, cats, wildlifeDetection of anti-Toxoplasma antibodies


IFAT (in house)

O.I.E. Terrestrial Manual

(2017) Toxoplasmosis

(Chapter 2.9.9)

131 5Blood serum samples of

horses

Detection of anti-Trypanosoma equiperdum

antibodies


IFAT (in house)


(2013) Dourine (Chapter

2.5.3.)


horsesDetection of anti-Theileria equi antibodies


IFAT (in house)


(2014) Equine

Piroplasmosis (Chapter

2.5.8.)


horsesDetection of anti-Babesia caballi antibodies


IFAT (in house)


(2014) Equine

Piroplasmosis (Chapter

2.5.8.)

134 8


bovine, sheep, goats, horses,

dogs

Detection of anti-Anaplasma phagocytophilum

antibodies


IFAT (in house)


(2015) Bovine

Anaplasmosis (Chapter

2.4.1.)

Parasites (Trichinella, Echinococcus, Anisakis) Legal basis: Ministerial Decision 3865/128713/22.10.2013

Honeybee health Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)

Bluetongue disease surveillance program (identification of insects vectors) Legal basis: Ministerial Decision 173/5333/17.01.2017(Β'235)



135 9 Blood serum samples of pigs Detection of anti-Trichinella spp antibodies Indirect Enzyme-linked Immunosorbent

Assay (ELISA)

Protocol developed and

validated by the European

Union Reference


136 10Blood serum, plasma and meat

juice of horse, pig, dogs and

wild boar

Detection of anti-Trichinella spp antibodies IndirectEnzyme-linked Immunosorbent

Assay (ELISA)Commercial kit ELISA

137 11 Trichinella larvaeIdentification of Trichinella Muscle Stage

Larvae at species level Multiplex PCR



Union Reference


138 12Intestinal mucosa of the

definitive hostDetection of Echinococcus spp. adult worms Sedimentation and Counting technique (SCT)



Union Reference


139 13Faecal smears of all animals

and birds

Detection of parasites (eggs, larvae, protozoan

oocysts or cysts etc)Microscopy/Sendimentation/ Flotation

140 14 Blood smears of all animals Detection of all parasites Microscopic examination of blood stained

with Giemsa-May Grunwald stain


(Protocol and reagents

depending on the parasite)

141 15

Faecal smears of livestock,

poultry, game birds and

companion animals

Detection of Cryptosporidium spp oocysts Acid-fast Ziehl-Neelsen stain


(May 2016)

Cryptosporidiosis (Chapter

2.9.4.)

142 16

Tissues (tongue, sceletal

muscles, peritoneal cavity,

liver, brain) of living animal

and post-mortem examination

or meat inspection of farmed

and wild animals

Diagnosis of Cysticercosis (Detection of larval

stages of cestodes-metacestodes- of Taenia

solium,T. saginata, T.ovis, T. hydatigena and T.

multiceps)

Visual inspection and microscopic

examination of cysts


(May 2014) Cysticercosis

(Chapter 2.9.5.)

143 17Individual identification of

insects

Identification of insects vectors of infectious

diseases of animals (eg. Culicoides spp,

Phlebotomine sandflies , Mosquitoes )

1. Morphological identification by

stereoscopic examination 2. Morphological

Identification of insects by microscopic

examination after preparation of slides

144 18 Abdomens of beesDetection of microsporidia Nosema spp

sporesSpore counts in haemacytometer


(May 2013) Nosemosis of

honey bees (Chapter 2.2.4.)

145 19 Bees, brood and debris Infestation of honey bees with Varroa sppExamination (morphological identification)

with the naked eye


(May 2008) Varroosis of



146 20Honey bee brood, bees, hive

debris

Infestation of honey bees with mite

Tropilaelaps spp

Morphological Identification under a

microscope


(May 2017) Infestation of

honey bees with

Tropilaelaps spp (Chapter

2.2.6.)

147 21Adult Aethina tumida in

coloniesInfestation of colonies with Aethina tumida

Morphological Identification under a

stereomicroscope


(May 2017) Infestation

with Aethina tumida ( small

hive beetle) (Chapter

2.2.5.)

148 22 Larval smearsInfection of honey bees with Melissococcus

plutonius (European foolbrood)

Microscopic examination of larvae smeared

on slides and stained with Carbol fuchsin


(May 2016) European

Foolbrood of honey bees

(Infection with

Melissococcus plutonius)

(Chapter 2.2.3.)

149 23 Larval smearsInfection of honey bees with Paenibacillus

larvae (American foolbrood)

Microscopic examination of larvae smeared

on slides and stained with Gram or Carbol

fuchsin stain


(May 2016) American


(Infection with

Paenibacillus larvae)

(Chapter 2.2.2.)

150


151

152 A.3 DVCA/DEPARTMENT OF MOLECULAR DIAGNOSTICS, FOOT & MOUTH DISEASE, VIRAL, RICKETTSIAL AND EXOTIC DISEASES153

154 25, Neapoleos Street, 153 10 Agia Paraskevi155 tel:+30 210 6007016, fax:+30 210 6082085156 [email protected]

158 NATIONAL REFERENCE LABORATORY159 #160 #161 #162 #163 #164 #165 #166 #167 #168 #169 #170 #

171 #172 #173 #174


177 #

178 #

179 #

180 #181




ruminants or swine

Antibody detection against the foot-and-mouth

virus

Blocking ELISA KIT PRIOCHECK FMDV NS BY

PRIONICS (7610440)

OIE Terrestrial Manual

2017 (Chapter 2.1.8)ESYD 695-2 15/7/2018


swine

Antibody detection against the classical swine

fever virus

Blocking ELISA KIT HERDCHEK CSFV Ab BY

IDEXX (9943220)

Dir2001/89

Dec2002/106ESYD 695-2 15/7/2018

187 3Blood serum or plasma

originating from ruminants

Antibody detection against the bluetongue

virus

Competitive ELISA for bluetongue (BLUE

TONGUE COMPETITION KIT BY IDVET) (BTC-

4P)

OIE Manual of Diagnostic


Terrestrial Animals 2017

(Chapter 2.1.3)

ESYD 695-2 15/7/2018

188 4Blood serum ,milk from

bovinesDetection of bovine leucosis antibodies

Elisa technique method using kit SVANOVIR

BLV-gp 51 code no10-2351-50 /10235102


2017 (Chapter 2.4.10)ESYD 695-2 15/7/2018

189 5

Central nervous system tissue

removed from the cranium of

almost every warm-blooded

animal

Antigen detection of rabies virus Fluorescent antibody test (FAT) for rabies




(Chapter 2.1.17.)

ESYD 695-2 15/7/2018

190 6

Blood serum samples or

plasma or clot or filter paper

blood samples of

domesticated and wild

animals including red foxes

Antibody detection against rabies virus Blocking ELISA (BioPro Rabies ELISA Ab)

Protocols recommended by


Laboratory (EURL)

ESYD 695-2 15/7/2018

191

Bluetongue disease

Epizootic haemorrhagic disease of deer

Legal basis: Directive2002/60/EC, Presidential Decrees 37/2005(Α'56), Ministerial Decision 260918/14.01.2009(Β'75)

Avian influenza

Honeybee health

Legal basis: Ministerial Decision 4411/138539/22.12.2017 (Β'4669)

Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)

Legal basis:Directive 92/66/EEC, Presidential Decree 141/1996(Α'108), Ministerial Decision 279719/19.11.2004(Β'1760)

Bovine Spongiform Encephalopathy & Small ruminants Transmissible

Spongiform EncephalopathiesLegal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)

Legal basis: Directives 2001/89/EC, Presidential Decrees 39/2005 (Α'57), Ministerial Decision 259/10562/30.01.2017(Β'449)

Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 259/10562/30.01.2017(Β'449)

Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 259/10562/30.01.2017(Β'449)

Legal basis: Directive 2000/75/EC, Presidential Decree 33/2003(Α'34), Ministerial decision 173/5333/17.01.2017(Β'235)

Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)

Legal basis: Directive 2003/99/EC, Presidential Decree 41/2006(A'44), Joint Ministerial Decision 331/10301/25.01.2013(Β'198)


Legal basis: Directive 92/35/EEC, Presidential Decree 33/1994(A'25)


Peste des petits ruminants (PPR)

Vesicular stomatitis

Classical swine fever

Swine vesicular disease

Lumpy Skin Disease

African swine fever

Rabies

Capripoxvirus induced diseases

African horse sickness

Rift valley disease

Foot and Mouth disease

Legal basis: Ministerial Decision 27/3259/14.01.2015(Β'142)West Nile fever


Legal basis: Directive 2003/85/EC, Presidential Decree 32/2007(Α'30)


Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004

Rinderpest Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)

Bovine leucosis

Newcastle disease





195 7

Whole blood with EDTA, skin

biopsy samples and cotton

swabs of nasal, ocular or saliva

bovine samples

Detection of Lumpy Skin Disease Virus (LSDV)

and differentiation from vaccine viral strainsREAL-TIME PCR In house method Validated

196 8 Serum, Plasma

ELISA immunosorbent assay for the detection

of antibodies against Peste de Petits Ruminants

(PPR)

COMPETITIVE ELISA FOR DETECTION OF

ANTI-PPR Ab ME TO KIT ID Screen PPR

Competition ΤΗΣ IDVET



Terrestrial Animals, 2017

Chapter 2.7.10.

197 9Blood serum from farm and

wild birds


of antibodies against Avian Influenza Type A

virus

Kit ELISA IDEXX (Avian Influenza MultiS-

Screen) farm and wild birds




Chapter 2.3.4

198 10Whole blood, bowels from

ruminantsDetection of Bluetongue virus genome Real Time PCR, conventional PCR




(Chapter 2.1.3.)



Laboratory (EURL)

199 11 Equine blood plasma, serumDetection of African Equine Sickness virus

antibodiesELISA



Laboratory (EURL)

200 12 Equine whole bloodDetection of African Equine Sickness virus

genomeReal Time PCR, conventional PCR




(Chapter 2.5.1.)



Laboratory (EURL)

201 13Blood plasma, serum of

ruminants and deers

Detection of Epizootic haemorrhagic disease

virus antibodiesELISA




(Chapter 2.1.7.)



Laboratory (EURL)

202 14Blood plasma of ruminants

and deers

Detection of Epizootic hemorrhagic disease

viral RNAReal Time PCR



Laboratory (EURL)

203 15 Equine blood plasma, serumDetection of Equine West Nile fever virus

antibodies

West Nile competition multi-species ELISA

and West Nile IgM capture ELISA




(Chapter 2.1.24.)



Laboratory (EURL)

204 16 Equine whole bloodDetection of West Nile Fever viral RNA in

equidaeReal Time PCR, conventional PCR




(Chapter 2.1.24.)



Laboratory (EURL)

205 17Mandible and teeth sections of

red foxes

Tetracycline detection and red fox age

determinationMicroscopy



Laboratory (EURL)

206 18

Central nervous system (CNS)

tissue removed from the

cranium

Detection of Rabies viral RNA REAL-TIME PCR, CONVENTIONAL PCR



Laboratory (EURL)


207 19 Equine serumDetection of Equine infectious anaemia virus

antibodiesAgar gel immunodiffusion




Chapter 2.5.6.

208 20 Equidae serumDetection of antibodies against Equine

Infectious Anaemia virusELISA




Chapter 2.5.6.

209 21Skin lesions, blood samples,

swabsticks

Detection of Capripoxvirus (SPPV,GTPV, LSD)

genomeReal Time PCR, conventional PCR




(Chapter 2.4.13.)



Laboratory (EURL)

210 22Serum of ovine and caprine

animalsDetection of Sheep Pox virus antibodies AGAR GEL IMMUNODIFFUSION




(Chapter 2.7.13.)

211 23 Skin biopsy samples Cell culture isolation of Sheep Pox virus VIRUS ISOLATION IN CELL CULTURES




(Chapter 2.7.13.)

212 24Blood serum, plasma from

companion animals

Titration of antibodies against Rabies virus in

companion animals

FAVN: Fluorescent Antibody Virus

Neutralization




(Chapter 2.1.17)

213 25 Serum or plasma of equidaeDetection of antibodies against West Nile

Fever virus in equidae

WEST NILE COMPETITION MULTI-SPECIES

ELISA and WEST NILE IgM CAPTURE ELISA




(Chapter 2.1.24.)



Laboratory (EURL)

214 26Whole blood with EDTA of

equidae

Detection of West Nile Fever viral RNA in

equidaeREAL-TIME PCR, CONVENTIONAL PCR



Laboratory (EURL)

215 27 PCR product

Sequencing of E gene & NS5, of Flavivirus &

phylogenetic analysis GEL BASED PCRs, SEQUENCING,

BIOINFORMATICS




(Chapter 2.1.24.)



Laboratory (EURL)

216 28 Serum or plasma of bovidaeDetection of antibodies against Infectious

Bovine Rhinotracheitis herpesvirus ELISA




(Chapter 2.4.12)

217 29 Serum or plasma of bovidaeDetection of antibodies against Bovine Viral

Diarrhoea virusesELISA




(Chapter 2.4.7)

218 30 Equidae serumDetection of antibodies against equine arteritis

virusELISA




(Chapter 2.5.10)

219 31 Equidae serum

Detection of antibodies against Equine

rhinopneumonitis viruses (equidae

herpesvirus-1 and -4)

ELISA




(Chapter 2.5.9)

220 32 Liver of rabbitsDetection of antibodies against Rabbit

haemorrhagic disease virusELISA




(Chapter 2.6.2)

221 33Liver, spleen, trachea of

rabbitsDetection of Myxomatosis viral DNA CONVENTIONAL PCR




(Chapter 2.6.1)


animals

Detection of antibodies against Caprine

arthritis-encephalitis (CAE) and maedi-visna

(MV) viruses

ELISA




(Chapter 2.7.2/3)

223 35 Serum of ovine

Detection of antibodies against Chlamydophila

abortus (Ovine chlamydiosis - Enzootic

Abortion of Ewes)

ELISA




(Chapter 2.7.6)

224 36 Fetus or aborted placentae



Abortion of Ewes)

REAL-TIME PCR





225 37 Fetus or aborted placentae



Abortion of Ewes)

STAINING IN SMEARS




(Chapter 2.7.6)


animals

Contagious

EcthymaAGAR GEL IMMUNODIFFUSION




227 39 Skin lesionsContagious

EcthymaCONVENTIONAL PCR




228 40 PCR productSequencing of VIR gene of ORF virus &

phylogenetic analysis

GEL BASED PCRs, SEQUENCING,

BIOINFORMATICS




229 41 Serum or plasma of ruminantsDetection of antibodies against Rift Valley

Fever virusELISA




(Chapter 2.1.18)

230 42Blood serum, plasma from

bovinesDetection of bovine leucosis antibodies

Agar gel immunodiffusion (IDEXX ΚΙΤ

POURQIER AGID LEUKOSIS)




(Chapter 2.4.10)

231 43Blood plasma, serum from

pigs and wildboars

Detection of Swine vesicular disease virus

antibodies

Competitive double sanwitch ELISA

(PRIONICS KIT PRIOCHECK SVDV Abs) or

Competitive ELISA for anti-SVD Abs (IDVET

KIT ID Screen Swine Vesicular Disease

Competition)




(Chapter 2.8.8)

232 44Brain tissue of adult

ruminantsDitection of prion protein TeSeE SAP Kits ELISA BIORAD




(Chapter 2.4.5) par 1.3.1233 45 Allantoic fluid from chicken

embryonated eggs

Haemagglutination test for the detection of

haemaggluitinatined factor and Haemagglutination test OIE Manual of Diagnostic

Tests and Vaccines for 234 46 Chicks (live and dead), swabs,

farm and wild birds

Detection of matrix, H5, H7 and Ν1 genes of

Avian Influenza virus Real Time PCR OIE Manual of Diagnostic

Tests and Vaccines for 235 47 Chicken embryonated eggs Avian Influenza virus isolation -inoculation of

chicken embryonated eggsVirus isolation OIE Manual of Diagnostic

Tests and Vaccines for 236 48 Blood serum from farm and

wild birds


of antibodies against Avian Influenza Type A Kit IDEXX (Avian Influenza) chicken, turkey OIE Manual of Diagnostic

Tests and Vaccines for 237 49 Blood serum from farm and

wild birds

Haemagglutination Inhibition test for the

detection of antibodies to different subtypes of Haemagglutination Inhibition test OIE Manual of Diagnostic



238 50 PCR product

Nucleotide sequence determination of specific

regions of the genome of H5 &H7 Avian

influenza virus, cleavage site identification and

phylogenetic analysis.

Conventional PCR, Sequencing,

Bioinformatics.EURL protocols

239 51 Chicken embryonated eggsNewcastle disease virus isolation -inoculation

of chicken embryonated eggsVirus isolation




Chapter 2.3.4 and EURL

protocols

240 52Allantoic fluid from chicken

embryonated eggs


haemaggluitinatined factor and

haemagglutination inhibition test for

characterization of Newcastle disease virus

Haemagglutination test





protocols

241 53Blood serum from farm and

wild birds

Haemagglutination Inhibition test for the

detection of antibodies against Newcastle

disease virus

Haemagglutination inhibition test





protocols

242 54Chicks (live and dead), swabs,

farm and wild birds

Detection of Newcastle Disease viral RNA using

Real Time PCR.Real Time PCR





protocols

243 55 PCR product

Determination of the amino acid motif in 112-

117 of the fusion protein of the Newcastle

Disease virus.

Conventional PCR, Sequencing,

Bioinformatics.





protocols

244 56Blood serum of chickens and

turkeys. Newcastle Disease virus antibodies detection

Kit ELISA (Newcastle Disease virus) for

chickens and turkeys.




Chapter 2.3.14

245 57 Cell culture supernatant Detection of Clasical swine fever virus genome PCR



Terrestrial Animals (ch:

2.8.3).EURL CSF SOPs.

EURL protocols

246 58EDTA BLOOD, SERUM, TISSUE

SWINE SAMPLESDetection of African swine fever virus genome REAL-TIME PCR, GEL BASED PCR



Terrestrial Animals (ch:

2.8.1). EURL ASF SOPs.

247 59 PCR productsRabies virus nucleoprotein coding gene

sequencing and phylogenetic analysisSequencing, bioinformatic analysis EURL protocols

248 60 PCR productsSequencing of GPCR και RP030 genes of

Capripoxvirus and phylogenetic analysisSequencing, bioinformatic analysis

Published techniques

(Lamien et al 2011, Le Goff

et al 2009)

249 61 Pathologic material from

ruminants and swines

Detection of Foot and mouth disease virus

genomePCR EURL protocols

250


251

252 A.4 DVCA/DEPARTMENT OF PATHOLOGY OF AQUATIC ORGANISMS253

254 25, Neapoleos Street, 153 10 Agia Paraskevi255 tel: +30 210 6080838, fax: +30 210 6080838256 [email protected]

258 NATIONAL REFERENCE LABORATORY259 #260 #261

262 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:263 #264



268 1 Fish tissueIsolation and identification of viral

haemorragic septicaemia virus

ELISA KIT BY BIO-X DIAGNOSTICS (ΒΙΟ

Κ272/2)Dir88/2006 ESYD 695-2 15/7/2018

269 2 Fish tissueIsolation and identification of infectious

haemopoetic necrosis virus

ELISA KIT BY BIO-X DIAGNOSTICS

(BIOK273/2)Dir88/2006 ESYD 695-2 15/7/2018

270 3Fish tissue /supernatant

tissue culture

Isolation and identification of koi herpes virus

(khv)

PCR using extraction kit of QIAGEN, (69504

DN EASY)Dir88/2006 ESYD 695-2 15/7/2018

271



275 4Fish tissue /cell culture

supernatant

Isolation and identification of epizootic

necrosis virus

Identification with conventional PCR,

sequencing and bioinformatics analysisDir88/2006,Dec1554/2015


supernatant

Isolation and identification of infectious

salmon anemia virus

Identification with conventional PCR,

sequencing and bioinformatics analysisDir88/2006,Dec1554/2016

277 6 Crustaceans tissue Identification of Taura syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2017

278 7 Crustaceans tissue Identification of Yellow head syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2018

279 8 Crustaceans tissue Identification of White spot syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2019

280 9 Crustaceans tissueIdentification of Aphanomyces astaci (Crayfish

plague)Identification with conventional PCR

OIE Aquatic animal health

diagnostic manual- OIE

EURL Protocol

281 10 Bivalve molluscs tissue Identification of Bonamia exitiosa parasite Identification with conventional PCR Dir88/2006,Dec1554/2020

282 11 Bivalve molluscs tissue Identification of Perkinsus marinus parasite Identification with conventional PCR Dir88/2006,Dec1554/2021

283 12 Bivalve molluscs tissue Identification of Microcytos mackini parasite Identification with conventional PCR Dir88/2006,Dec1554/2022

284 13 Bivalve molluscs tissue Identification of Marteilia refringens parasite Identification with conventional PCR Dir88/2006,Dec1554/2023

285 14 Bivalve molluscs tissue Identification of Bonamia ostreae parasite Identification with conventional PCR Dir88/2006,Dec1554/2024


supernatant

Isolation and identification of sea bass nervous

necrosis virus

Identification with indirect

immunofluorescence, identification with

conventional PCR or real-time PCR (PCR ή

qPCR)



EURL Protocol

Fish diseases

Diseases of crustaceans

Legal basis: Directive 2006/88/ΕΚ, Presidential Decree 28/2009(Α'46), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)

Legal basis: Directive 2006/88/ΕΚ, Presidential Decree 28/2009(Α'46), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)

Diseases of bivalve molluscs Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)


tel:+30 210 6080838, fax:+30 210 6080838



supernatant

Isolation and identification of red bream

iridovirusIdentification with conventional PCR



Reference Laboratory

protocol


supernatant

Isolation and identification of lymphocystis

iridovirus

Identification with conventional PCR or real-

time PCR (PCRor qPCR)

Published methods

(E.J.Valverde et al. 2016,

H.Kvitt et al.2008)

289 18 Fish tissueIsolation and identification of Photobacterium

damsella subsp. Piscicida microorganism

Identification with biochemical method

(API), Identification with serological method

(agglutination), Identification with

conventional PCR or real time PCR (qPCR)

Published methods

(C.R.Osorio et al.2000,

R.Carraro et al. 2017)

290 19 Fish tissueIsolation and identification of Listonella

anguillarum microorganism


(API), Identification with serological method

(agglutination on plate), Identification with

conventional PCR

Published methods

(F.Crisafi et al.,2011)

291 20 Fish tissueIsolation and identification of Aeromonas

veronii biovar sobria microorganism


(API), Identification with conventional PCR

Published methods

(M.Smyrli , A.Prapas et al.

2017)

292 21 Fish tissueIsolation and identification of Tenacibaculum

maritimus microorganismIdentification by conventional PCR

Published methods

( R.Avendano-Herrera et al.

2006)

293 22 Fish tissueIsolation and identification of trout Yersinia

ruckeri microorganism



Published methods (J.T.

Lejeune et al. 2000)

294 23 Fish tissueIsolation and identification of trout

Lactococcus garviae microorganism



Published

methods(A.Zlotkin et

al.1998)

295 24 Fish tissueIsolation and identification of salmonid

Aeromonas salmonicida microorganism



Published methods

(H.K.Byers et al. 2002, C.

Fernandez-Alvarez et al.

2016)

296 25 Fish tissueIsolation and identification of Edwardsiella

tarda microorganism



Published methods

(P.Katharios et al. 2015)

297 26 Fish tissueIsolation and identification of Flavobacterium

psychrophilum (Rainbow trout fry syndrome)



Published methods

(T.Wiklund et al. 2000)

298 27 Fish tissueIdentification of monogenic trematoda

parasitesMicroscopy In house method

299 28 Fish tissue Identification of digenic trematoda parasites Microscopy In house method

300 29 Fish tissue Identification of crustacean parasites Microscopy In house method

301 30 Fish tissueIdentification of myxopsporea and parasite

myxosporeaMicroscopy, preparations staning In house method

302 31 Fish tissueIsolation and identification of nematode

parasites of Anisakidae familyArtificial digestion and multiplex PCR

Published methods

(B.R.Dixon,2006)303


304

305 A.5 DVCA/DEPARTMENT OF FOOD HYGIENE306

307 25, Neapoleos Street, 153 10 Agia Paraskevi308 tel: +30 210 6399241, fax: +30 210 6399241309 [email protected]

311 NATIONAL REFERENCE LABORATORY312 #313 #314 #315

316 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:317 #318 #319 #320 #321 #322



326 1 Honey Determination of diastase index AOAC 958.09:2005, revised January 2010 Dir2001/110 ESYD 05-4 5/9/2020

327 2 Honey Determination of free acidity AOAC 962.19:2005 Dir2001/110 ESYD 05-4 5/9/2020

328 3 HoneyDetermination of hydroxymethylfurfural

(HMF)AOAC 980.23:2005 Dir2001/110 ESYD 05-4 5/9/2020

329 4 Honey Pollen identification and counting MD-39 Pollen method according to

International Honey Committee

Joint Ministerial Decision

127/2004/18.02.2005

(Β΄239)

ESYD 05-4 5/9/2020

330 5 Honey Determination of electrical conductivityMD 56 Harmonised Methods of the

International Honey Commission (2009),

Method 2

Dir2001/110 ESYD 05-4 5/9/2020

331 6 Honey Determination of moistureMD 55 Harmonised Methods of the

International Honey Commission (2009),

Method 2

Dir2001/110 ESYD 05-4 5/9/2020

332 7Foods for human consumption

and animal feeding stuffsDetection of Listeria monocytogenes ISO 11290-1:2017 Reg2073/2005 ESYD 05-4 5/9/2020


and animal feeding stuffsDetection of Salmonella spp ISO 6579-1:2017 Reg2073/2005 ESYD 05-4 5/9/2020


and animal feeding stuffsDetection Vibrio parahaemolyticus ISO 21872-1: 2017 Reg2073/2005 ESYD 05-4 5/9/2020

Viral and bacteriological contamination of bivalve molluscs

Listeria monocytogenes

Coagulase positive staphylococci, including Staphylococcus aureus

Legal basis: Ministerial Decision 3865/128713/22.10.2013



Milk and milk products (criteria for raw milk)

Legal basis: Directive 2001/110/ΕC, Presidential Decree 97/2017 (Α'138), Joint Ministerial Decision 127/2004/18.02.2005 (Β΄239)

Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016

Legal basis: Desicion 2013/652/ΕU, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)

Legal basis: Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)

Microbiological criteria for foodstuffs Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)

Pollen content and other composition criteria of honey

Animal by-products

Antimicrobial resistance


tel:+30 210 6080838, fax:+30 210 6080838



and animal feeding stuffs

Detection of the thermotolerant

Campylobacter sppISO 10272-1:2006 Reg2073/2005 ESYD 05-4 5/9/2020



Enumeration of coagulase-positive

staphylococciISO 6888-2:1999 Reg2073/2005 ESYD 05-4 5/9/2020


and animal feeding stuffsEnumeration of microorganisms at 30οC ISO 4833-1:2013 Reg2073/2005 ESYD 05-4 5/9/2020



Enumeration of β-glucoronidase positive

Escherichia coliISO 16649-2:2001 Reg2073/2005 ESYD 05-4 5/9/2020


and animal feeding stuffsEnumeration of Enterobacteriaceae ISO 21528-2:2017 Reg2073/2005 ESYD 05-4 5/9/2020




Escherichia coli in live bivalve molluscs

ISO 16649-3:2015Reg2073/2005 ESYD 05-4 5/9/2020



Enumeration of Listeria monocytogenesISO 11290-2:2017 Reg2073/2005 ESYD 05-4 5/9/2020



Enumeration of coagulase-positive

staphylococci

ISO 6888-1:1999Reg2073/2005 ESYD 05-4 5/9/2020

343 18 Milk and milk products Count of yeast and moulds at 25 οC ISO 6611:2004 Reg2073/2005 ESYD 05-4 5/9/2020

344 19 Milk and milk productsDetection of staphylococcal enterotoxins type

SEA to SEEISO 19020:2017 Reg2073/2005 ESYD 05-4 5/9/2020

345 20 Meat and meat productsDetection of staphylococcal enterotoxins type

SEA to SEEISO 19020:2017 Reg2073/2005 ESYD 05-4 5/9/2020

346



350 21 Canned foods Incubation test at 30oC & 55oC APHA 1992, Compendium Methods 3rd edAPHA 1992, Compendium

Methods 3rd ed


and animal feeding stuffsEnumeration of Coliforms ISO 4832:2006 ISO 4832:2006



Enumeration of Sulfite-reducing bacteria (or

clostridia)ISO 15213:2003 ISO 15213:2003


and animal feeding stuffsEnumeration of Enterococci

APHA, Compendium of Methods for the

Microbiological Examination of Foods

APHA, Compendium of

Methods for the

Microbiological

Examination of Foods



Enumeration of yeasts and moulds (products

with aw>0,95)ISO 21527-1:2008 ISO 21527-1:2008


and animal feeding stuffsEnumeration of Lactic Acid Bacteria

ISO 15214:1998

ISO 7889:2003 (IDF 117: 2003)

ISO 15214:1998

ISO 7889:2003 (IDF 117:

2003)356 27 Shellfish bivalves Detection and enumeration of norovirus ISO 15216-2:2013 ISO 15216-2:2013 Validated

357


358

359 A.6 DVCA/DEPARTMENT OF TOXICOLOGY, RESIDUES & ENVIRONMENTAL CONTAMINANTS360

361 25, Neapoleos Street, 153 10 Agia Paraskevi362 tel: +30 210 6013899, fax: +30 210 6012594363 [email protected] 364


366

#

367



371 1 Meat, liver and kidney Determination of cadmium (Cd) content ΜD 01 In-house method using Atomic

Absorbtion Spectrometry with Graphite

Furnace (ZET-AAS)

Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

372 2 Fisheries and fishery products Determination of cadmium (Cd) content ΜD 02 In-house method using Atomic

Absorbtion Spectrometry with Graphite

Furnace (ZET-AAS)

Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

373 3Fisheries and Fishery

productsDetermination of mercury (Hg) content

ΜD 06 In-house method using Atomic

Absorption Spectrometry with Flow Injection

– Mercury Hydride (FI-MH-AAS)

Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

374 4 FisheriesDetermination of dyes: Malachite Green,

Leucomalachite Green, Crystal Violet,

Leucocrystal Violet, Brilliant Green

MD-21 In house LC-MS/MS method,

validated according to the requirements of

the Decision EU/657/2002.

Dec.657/2002 ESYD 05-4 5/9/2020

375 5 Animal tissues

Determination of tetracycline residues (4):

Tetracycline, Oxytetracycline,

Chlortetracycline, Doxycycline, 4-

Εpichlortetracycline

MD-04 In house method based on the

corresponding method of the European

Community Reference Laboratory ANSES

using High Performance Liquid

Chromatography -Mass Spectrometry (LC-

MS) (ESI-positive mode), validated according

to the requirements of the Decision

EU/657/2002.

Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020

376 6 Muscular tissues

Determination of quinolone residues (7):

Ciprofloxacin, Danofloxacin, Difloxacin,

Enrofloxacin, Flumequine, Marbofloxacin,

Oxonilic acid





Chromatography with FLD (HPLC-FLD),



Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020


Determination of sulfonamide residues (10):

Sulfadiazine, sulfathiazole, sulfamerazine,

sulfadimidine, sulfamethoxypyridazine,

sulfamonomethoxine, sulfachlorpyridazine,

sulfadoxine, sulfadimethoxine, Na-sulfaclozine





Chromatography with DAD (HPLC-DAD),



Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020

Residues of substance groups Α2, Α5, Α6 (chloropromazine, nitroimidazoles),

Β1 (apart from honey), Β2α, Β2b, Β2d, Β2e, Β3b, B3c, B3d, B3e of Annex I of

Directive 96/23/ECLegal basis: Desicion 98/536/ΕC


tel:+30 210 6080838, fax:+30 210 6080838


378 8 Milk


Sulfadiazine, sulfamerazine, sulfadimidine,

sulfamethoxypyridazine, sulfachlorpyridazine,

sulfadoxine, sulfadimethoxine





Chromatography with Fluorescence Detector

(HPLC-FLD), validated according to the

requirements of the Decision EU/657/2002.

Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020

379 9 Milk Determination of Cd and PbMD-05 In house method using AAS, graphite

furnace technique (ZETAAS)Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

380 10 Eggs


Sulfadiazine, sulfathiazole, sulfamerazine,

sulfamethoxypyridazine, sulfamethazine,

sulfachlorpyridazine, sulfadoxine,

sulfadimethoxine

MD-31 In house method using High

Performance Liquid Chromatography –

Tandem Mass Spectrometry (LC-MS) (ESI-

positive mode), validated according to the

requirements of the Decision EU/657/2002

Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020

381 11 Urine, water

Determination of β-antagonists residues (17):

zilpaterol, terbutalin, salbutamol, cimaterol,

cibuterol, fenoterol, ritodrin, clencyclohexerol,

clenproperol, clenbuterol, ractopamin,

isoxsuprin, tulobuterol, clenpenterol,

mabuterol, mapenterol, brombuterol



Community Reference Laboratory BVL using

High Performance Liquid Chromatography –

Tandem Mass Spectrometry (LC-MS/MS) (ESI-



Απόφ.ΕΕ 657/2002

Οδ.96/22/ΕΚESYD 05-4 5/9/2020


Determination of β-antagonists residues (17):

zilpaterol, terbutalin, salbutamol, cimaterol,

cibuterol, fenoterol, ritodrin, clencyclohexerol,

clenproperol, clenbuterol, ractopamin,

isoxsuprin, tulobuterol, clenpenterol,

mabuterol, mapenterol, brombuterol



Community Reference Laboratory BVL using

High Performance Liquid Chromatography –

Tandem Mass Spectrometry (LC-MS/MS) (ESI-



Dec.657/2002

Dir.96/22/ΕCESYD 05-4 5/9/2020


Determination of tranquilizers (antianxiety

drugs) residues (7): acetopromazine,

chloropromazine, xylazine, promazine,

propionylopromazine, carazolol, azaperone,

Azaperol

MD-03 In house method using High

Performance Liquid Chromatography –

Tandem Mass Spectrometry (LC-MS/MS),



Dec.657/2002 ESYD 05-4 5/9/2020

384 14 Animal urine

Detection and determination of (6) antithyroid

drugs (thiouracil, methyl thiouracil, propyl

thiouracil, phenyl thiouracil, tapazol,

mercaptobenzimidazol)

MD-23 In house method using LC-MS/MS

after derivatization with 3-iodo-benzyl-

bromide.

Dec.657/2002 ESYD 05-4 5/9/2020

385 15Foods of animal origin and

their productsDetermination of lead (Pb) content

ΜD-08 In – house method using Atomic

Absorbtion Spectrometry with

electrothermal Graphite Furnace.

Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

386 16 Thyroid and animal tissues

Detection and determination of (6) antithyroid

drugs (thiouracil, methyl thiouracil, propyl

thiouracil, phenyl thiouracil, tapazol,

mercaptobenzimidazol)

MD-32 In house method using LC-MS/MS

after derivatization with 3-iodo-benzyl-

bromide.

Dec.657/2002

Dir.96/22/ΕCESYD 05-4 5/9/2020

387 17 Meat and blood serum Determination of nitroimidazolesMD-36 In house LC/MS-MS method based on

Community Reference Laboratory BVL

method

Dec.657/2002

Dir.96/23/ΕCESYD 05-4 5/9/2020

388 18 Tissue, milk and honeyDetermination of organophosphorus pesticide

residues

MD 38 In house LC-MS/MS method according

to SANCO 12495/2011 document

Reg.396/2005,

Reg.37/2010ESYD 05-4 5/9/2020

389 19 Milk Quantitative determination of aflatoxin M1Code: MD-53 ISO 14501/IDF 171:2007

Immunoaffinity column HPLC / FLD methodDec.657/2002 ESYD 05-4 5/9/2020

390 20 Animal tissues Quantitative determination of ochratoxin AIn house validated method Code: MD-54

Immunoaffinity column HPLC / FLD methodDec.657/2002 ESYD 05-4 5/9/2020


391 21 Milk and Muscle

Quantitative dete rmination of Avermectins

(Moxidectin, Abamectin, Emamectin,

Doramectin, Ivermectin)

Code: MD-46 In house method based on the


Union Reference Laboratory BVL using High

Performance Liquid Chromatography with

Fluorescence Detector (HPLC-FLD), validated

according to the requirements of the

Decision EU/657/2002.

Dec.657/2002

Reg.37/2010ESYD 05-4 5/9/2020

392 22 Animal tissues and milk

Detection of antimicrobial agents (quinolones,

cephalosporins, penicillins, macrolides,

tetracyclines, aminoglycosides, sulfonamides)

Code: MD-24 In house method based on

LMV/RMV/BIO/P TC/15.AN Version 06/17-

10-2006 Guidance of Community Reference

Laboratory ANSES

Reg.37/2010 ESYD 05-4 5/9/2020

393 23 Honey 4. Determination of Cd and Pb MD-09 In house Z-ETAAS method Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020

394



398 24 Milk Determination of tetracycline residues HPLC-DADDec.657/2002

Reg.37/2010Validated

399 25 EggsDetermination of antimicrobial residues (b-

lactames, quinolones, macrolides,

sulfonamides, tetracyclines)

LC-MS/MSDec.657/2002


400 26 Tissue and MilkDetermination of antimicrobial residues (b-

lactames, quinolones, macrolides)LC-MS/MS

Dec.657/2002


401 27 Meat, Milk, Kidney Determination of aminoglycosides LC-MS/MSDec.657/2002


402 28Animal tissues (meat, kidney),

milk, fisheries, eggs

Determination of antimicrobial agent residues

(post- screening)Biosensors

Dec.657/2002


403 29Animal tissues, milk, drinking

water

Determination of β-antagonists residues

(screening)ELISA

Guidance of European

Laboratories based on Dec.

657/2002

Validated

404


405

406 A.7 DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHALKIS407

408 Pei Dokou, Chalkida. P.O. Box 19241, 34100 Chalkida409 tel: +30 2221042521, fax: +30 2221041421410 [email protected]

412 NATIONAL REFERENCE LABORATORY413 #414 #415




426 1Foodstuffs and animal feeding

stuffsDetection of Salmonella spp. ISO 6579:2002 Reg2073/2005 ESYD 477-3 17/11/2020


stuffs

Enumeration of microorganisms by the colony

count method at 30 0 C, using the pour plate

technique

ISO 4833-1: 2013 Reg2073/2005 ESYD 477-3 17/11/2020


stuffs

Enumeration of microorganisms by the colony

count method at 30 0 C, using the surface

plating technique

ISO 4833-2:2013 Reg2073/2005 ESYD 477-3 17/11/2020


stuffs

Detection and enumeration of

Enterobacteriaceae, by the colony count

technique

ΙSO/TC 21528-2:2004 Reg2073/2005 ESYD 477-3 17/11/2020

430 5Live bivalve molluscs Enumeration of β-glucoronidase positive

Escherichia coli in

ISO 16649-3:2015Reg2073/2005 ESYD 477-3 17/11/2020

431 6

Animal faeces and

environmental samples from

the primary production stage

Detection of Salmonella spp. ISO 6579:2002/Amd1:2007 ANNEX D Reg2160/2003 ESYD 477-3 17/11/2020

432 7 Salmonella spp isolates Serotyping of Salmonella spp strains ISO 6579-3:2014 Reg2160/2003 ESYD 477-3 17/11/2020

433 8Salmonella spp & E.coli

isolates

Monitoring of Antimicrobial resistance of

Salmonella spp & E.coli strains with the Disk

Diffusion agar test to:Ampicillin, cefotaxime,

ceftazidime, gentamycin, nalidixic acid,

ciprofloxacin, chloramphenicol, tetracycline,

trimethoprim.

Antimicrobial Susceptibility–Agar Disk

Diffusion Test according to the valid editions

of CLSI:Μ2, CLSI:M100, Eucast Clinical

Breakpoint Table and article of Cavaco LM

and Aarestrup FM (J.Clin.

Microbiol.2009.Sep;47(9):2751-2758)

Dir2003/99/EC

Dec2007/407/ΕΚESYD 477-3 17/11/2020

434 9Salmonella spp & E.coli

isolates


Salmonella spp & E.coli strains with the

Minimum Inhibition Concetration (MIC) test in

microplate

Antimicrobial Susceptibility– Minimum

Inhibition Concetration (MIC) – Broth

Microdilution method using Sensititre

microplate manufactured by TREK

Diagnostic Systems, according to the valid

editions of guidelines CLSI:Μ7, CLSI:M100,

Eucast epidemiological cut off values and the

article of Cavaco LM and Aarestrup FM

(J.Clin. Microbiol. 2009.Sep;47(9):2751-

2758)

Dec2013/652 ESYD 477-3 17/11/2020

Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)


Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)


Microbiological criteria for foodstuffs

Zoonoses & zoonotic agents (Samonella)


Legal basis: Regulation (EC) 2160/2003, Presidential Decree 41/2006 (A'44), Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172),

Legal basis: Directive 2003/99/EC, Ministerial Decision 3865/128713/22.10.2013, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)


Animal by-products

Residues of substance group B1 of Annex I of Directive 96/23/EC

Honeybee health



435 10

Animal and poultry muscular

tissues and milk of bovine,

sheep and goats




In house method (ΜΔ-07) based on

LMV/RMV/BIO/P TC/15.AN Version 06/17-

10-2006 Guidance of Community Reference

Laboratory ANSES

Dec657/2002, Reg37/2010 ESYD 477-3 17/11/2020

436 11 Meat and poultry meat Detection of Campylobacter spp. ISO 10272-1: 2006 Reg 2073/2005 ESYD 477-3 17/11/2020

437 12Campylobacter jejuni &

Campylobacter coli isolates


Campylobacter jejuni & Campylobacter coli

strains with the Minimum Inhibition

Concetration (MIC) test in microplate.

Antimicrobial Susceptibility– Minimum

Inhibition Concetration (MIC) – Broth

Microdilution method, using Sensititre

microplate manufactured by TREK

Diagnostic Systems, according to the valid

editions of guidelines CLSI: VET 01-A4,

VET01S, Μ7, M100, Eucast epidemiological

Dec2013/652 ESYD 477-3 17/11/2020

438



442 13 FoodstuffsEnumeration of β-glucoronidase positive

Escherichia coli in ISO 16649-2:2001 Reg2073/2005

443 14Apis mellifera honey bees and

brood

Detection of Varroa destructor (Varroosis)

mites and their counting

Macroscopic & microscopic examination and

identification / OIE Terrestrial Manual 2008

2.2.7

OIE Terrestrial Animal

Health Code , Article 9.6

444 15 Forager bees Apis mellifera

Detection of Nosema spp. (spores) (qualitative

determination) and their count

(quantification)

Microscopic examination, spore count on

hemocytometer and determination of mean

spore number per bee / OIE Terrestrial

Manual 2013 2.2.4

MD

4424/137557/11.11.2013

445 16 Bees of Apis melliferaDetection of Acarapis woodi mites

(Acarapisosis)

Mιcroscopic observation & identification OIE

Terrestrial Manual 2008 2.2.1.


Health Code, Article 9.1.

446 17 Fresh meatIsolation of ESBL enzymes, AmpC and

carbapenemases-producing E. Coli strains

Protocol of DTU Food Laboratory-European

Reference Laboratory for Antimicrobial

Resistance

Dec2013/652

447 18 CaecumIsolation of ESBL enzymes, AmpC and

carbapenemases-producing E. Coli strains

Protocol of DTU Food Laboratory-European

Reference Laboratory for Antimicrobial

Resistance

Dec2013/652

448 19 CaecumDetection of Campylobacter spp (C.jejuni /

C.coli) ISO 10272-1 Dec2013/652

449 20 Cambylobacter spp strains Identification of Campylobacter spp

PCR testing protocol for the differentiation of

Cambylobacter spp isolates (C. Jejuni, C. Coli),

suggested by the European Reference

Laboratory for Antimicrobial resistance (2nd

ed, November 2013)

Dec2013/652

450 21 Enterobacteriaceae strains

Molecular testing for the detection of plasmids

mediating the transfer of colistin to

Enterobacteriaceae isolates

PCR testing protocol for the detection of

plasmids mediating the transfer of colistin to

Enterobacteriaceae isolates, suggested by

the European Reference Laboratory for

Antimicrobial resistance (2nd ed, October

2016)

Dec2013/652

451


452

453 A.8 DVCA/DEPARTMENT: VETERINARY LABORATORY OF TRIPOLIS454

455 Pelagos of Arcadia, 22100 Tripolis

456 tel: +30 2710 226849, fax: +30 2710 232909457 [email protected]


460 #

461

462 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:463 #464 #465 #466



470 1Milk, fish, eggs, meat and

honeyDetermination of chloramphenicol content

Modified method Afssa-Fougeres: “Methode

de confirmation des residues de

chloramphenicol dans les matrices d’origine

biologiqoue par lc/ms-ms”

Dec2002/657 ESYD 465-3 3/11/2020

471 2 Eggs, meat, fish and honey Determination of nitrofurane metabolites

Modified method Afssa-Fougeres:

“Determination de residues de nitrofuranes

(metabolites) dans les tissus par LC/MS-MS”

Dec2002/657 ESYD 465-3 3/11/2020

472 3 Honey

Detection and quantification of carbamates

Aldicarb,Carbaryl , Carbofuran ,Methomyl ,

Oxamyl ,Methiocarb ,Methiocarb Sulfoxide

,Pirimicarb,Pirimicarb

Desmethyl,Fenoxycarb,Propoxur

In house LC/MS-MS method validated

according to the Decision EC 657/2002

Dec2002/657,

SANTE/11945/2015ESYD 465-3 3/11/2020

473



477 4 Honey

Determination of pyrethroids Tau-

FLUVALINATE, DELTAMETHRIN, Lambda-

CYHALOTHRIN, B-CYFLUTHRIN, BIFENTHRIN,

CYPERMETHRIN (mixture of isomers),

PERMETHRIN (mixture of isomers),

FENPROPATHRIN

LC-MS/MSDec2002/657,

SANTE/11945/2015Validated

478 5 Drinking Water Determination of nitrofurane metabolitesModified method Afssa-Fougeres:

“Determination de residues de nitrofuranes

(metabolites) dans les tissus par LC/MS-MS”

Dec2002/657 Validated

479 6











Reg535/2002 Validated

480 7











Reg535/2002

481 8Milk samples of domestic or

wild ruminants



ELISA Test according to the Commission

Regulation 535/2002/EC amending Annex C

of the Directive 64/432/EEC and the

Decision 2000/330/EC

Reg535/2002


domestic or wild ruminants







Reg535/2002


bovine

Detection of antibodies against Bovine Leucosis

Virus


Directive 64/432/EC, the Decision

2009/976/ΕΕ and the OIE Manual of

Diagnostic Tests and Vaccines for Terrestrial

Dir64/432/ΕEC,

Dec2009/976/ΕU

484 11Blood serum samples of Sheep

& Goat

Detection of antibodies against Maedi Visna –

CAE virusesELISA


2017 Chapter 2.7.2/3


& Goat


abortus (Chlamydial Abortions in Sheep &

Goat)

ELISAOIE Terrestrial Manual

2015 Chapter 2.1.16.


& Goat


burnetii (Q Fever)ELISA


2012 Chapter 2.7.7


ruminantsDetection of antibodies Toxoplasma gondii ELISA


2017 Chapter 2.9.9.


ruminants

Detection of antibodies Mycobacterium Avium

subsp. Paratuberculosis ELISA


2014 Chapter 2.1.15.

Residues of substance groups Α6 (chloramphenicol, nitrofurans) and Β2c of

Annex I of Directive 96/23/ECLegal basis: Desicion 98/563/ΕC

Honeybee health


Bovine leucosis






489 16Blood smears of food-

producing animalsDetection of parasites

Microscopic examination of blood stained

with Giemsa




490 17Faecal smears of food-

producing animalsDetection of Cryptosporidium spp oocysts Modified Ziehl-Neelsen stain


2016 Chapter 2.9.4.

491 18

Sections of small/large


nodes - Faeces

Detection of Mycobacterium Avium subsp.

Paratuberculosis

Microscopic examination, Ziehl-Neelsen

Stain.


2014 Chapter 2.1.15.

492 19

Diagnostic Specimens and

Milk Samples of food-

producing animals

Detection of Pasteurella

multocida/Mannheimia haemolytica,

Staphylococcus spp., Streptococcus spp.,

Pseudomonas spp., Escherichia coli, Klebsiella

Culture, isolation, identification.

P.J.Quin, M.E. Carter,B.K.

Markey, G.R. Carter, Clinical

Veterinary Microbiology,

WOLFE, 1994.493 20 Brain Sections Detection of Listeria monocytogenes Culture, isolation, identification. ISO 11290-1:2017

494 21Lung tissue, synovial fluid,

milk samplesDetection of Mycoplasma spp. Culture, isolation, identification.


2013 Chapter 2.7.4.

495 22 Bacterial Pathogens Isolates

Monitoring of Antimicrobial Resistance of

Bacterial Pathogens with the Disk Diffusion

agar test.

Antimicrobial Susceptibility–Agar Disk


of CLSI:Μ2, CLSI:M100, Eucast.

CLSI M2 M100 EUCAST


brood.

Diagnosis of Varroosis / Infestation with

Varroa destructorMacroscopic & Microscopic Examination


2008 Chapter 2.2.7.

497 24 Apis mellifera honey bees Diagnosis of Nosemosis Microscopic ExaminationOIE Terrestrial Manual

2013 Chapter 2.2.4.

498 25 Apis mellifera honey beesDiagnosis of Acarapisosis / Infestation with

Acarapis WoodiMicroscopic Examination


2008 Chapter 2.2.1.

499 26 Apis mellifera BroodDiagnosis of European Foulbrood / Infestation

with Melissococcus Plutonius Microscopic Examination, identification.


2016 Chapter 2.2.3.

500 27 Apis mellifera BroodDiagnosis of American Foulbrood / Infestation

with Paenibacillus larvae Culture, isolation, identification.


2016 Chapter 2.2.2.


brood.

Infestation of Honey Bees with Tropilaelaps

spp.Macroscopic & Microscopic Examination


2017 Chapter 2.2.6.

502 29Apis mellifera honey bees,

brood and beehives.

Infestation with Aethina tumida (Small Hive

Beetle)Macroscopic Examination & Stereoscopy


2017 Chapter 2.2.5.

503 30 Apis mellifera honey bees Infestation with Malpighamoeba mellificae Microscopic Examination Bibliography

504 31 Apis mellifera brood. Infestation with Ascosphaera apis Microscopic Examination Bibliography

505 32 Apis mellifera brood. Infestation with Aspergillus flavus Microscopic Examination Bibliography

506


507

508 A.9 DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHANIA509

510 M. Mpotsari 66, Chania, Zip . 73136, Prefecture of Chania511 tel: +30 28210 96189, fax: +30 28210 88397512 [email protected] 513


515 #

516




523 1 HoneyDetermination of tetracyclines (ΟΤC, ΤC, CTC,

DTC)

In house Method (ΜΔ01) using HPLC

validated according to the Decision EC

657/2002

Dec2002/657 ESYD 481-4 13/11/2020

524



528 2 Honey Determination of streptomycine HPLC-FLD Dec2002/657 Validated

529 3 Honey Determination of sulfonamides HPLC-FLD Dec2002/657 Validated

530 4 Apis mellifera honey bees Average Nosema sp. spore count per sample Spore counts in a haemacytometer


2014 2.2.4 /

MD

4424/137557/11.11.2013


broodVarroa destructor infestation Macroscopic & microscopic examination


Health Code-Article 9.6


2014 2.2.7 /

MD

4424/137557/11.11.2013

532 6 Apis mellifera honey bees Tropilaelaps sp. infestation Macroscopic & microscopic examination




2014 2.2.6533 7 Apis mellifera honey bees Detection of Malpighamoeba mellificae Microscopy

534 8 Honey bees, brood Detection of Paenibacillus larvae (AFB) Culture-microscopy-catalase testing




2014 Chapter 2.2.2

Residues of substance group Β1 of Annex I of Directive 96/23/EC in honeyLegal basis: Desicion 98/536/ΕC




535 9 Honey bees, brood Detection of Melissococcus plutonius (EFB) Μicroscopy




2014 Chapter 2.2.3536 10 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy Beebook, coloss

537 11 Brood Detection of Aspergillus flavus Macroscopic observation & microscopy Beebook, coloss

538 12 Honey bees, brood, beehives Small Hive Beetle infestation Macroscopic observation & stereoscopyOIE Terrestrial Manual

2014 Chapter 2.2.5539 13 Adults, larvae Detection of Galleria mellonella Macroscopic observation Beebook, coloss

540 14 Adults, larvae Detection of Achroia grisella Macroscopic observation Beebook, coloss

541 15Sheep & goat whole blood or

blood serum

Detection of antibodies against Maedi Visna –

CAE viruses ELISA, Agar gel immunodiffusion test

O.I.E. / 4th ed.,2000


2017 Chapter 2.7.2/3


ruminants

Detection of mycobacterium avium spp.

paratuberculosis antibodies ELISA

Directive 64/432


2014 Chapter 2.1.15

543 17 Sheep and goat milkDetection of Mycoplasma agalactiae

(Contagious Agalactia)Culture & stereoscopy


2013, Chapter 2.7.4

544 18 Milk Mastitis testWhiteside test, culture, microscopy,

biochemical testing & antibiogram545


546

547 A.10 DVCA/DEPARTMENT: VETERINARY LABORATORY OF HERACLION548

549 Port of Heraklio, Pier 03, Heraklion, Crete, Greece

550 tel: +30 2810280869, fax: +30 2810341743551 [email protected] 552

553 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:554 #555 #556 #557 #558



562 1Foodstuffs and animal

feeding stuffsDetection of Salmonella spp. ISO 6579: 2002

Reg2073/2005

Reg1774/2002 ESYD 585-2 8/12/2017

563 2Foodstuffs and animal

feeding stuffsDetection of Listeria monocytogenes ISO 11290-1:1996/Amd1:2004

Reg2073/2005

Reg1441/2005

Reg365/2010

ESYD 585-2 8/12/2017

564 3






abortus, B. melitensis, B. suis





Dir64/432 ESYD 585-2 8/12/2017

565



569 4

Animal faeces and



Detection of Salmonella spp. ISO 6579:2002/Amd1:2007 ANNEX D

Reg2160/2003

Reg1003/2005

Reg1168/2006

Reg646/2007

Reg213/2009

570 5











Reg2073/2005

Reg1441/2005

Reg365/2010

571 6Food and Animal feeding

stuffs

Enumeration of coagulase positive

staphylococcusISO 6888-2:1999/ Amd1: 2003

Reg2073/2005

Reg1441/2005

Reg365/2010


stuffs

Enumeration of the total aerobic

microorganisms at 30 oC by the pour plate

technique

ISO 4833-1:2013

Reg2073/2005

Reg1441/2005

Reg365/2010

Dec1545/70158


stuffs

Enumeration of b- glucuronidase- positive

Escherichia coli in agarISO 16649-2:2001

Reg2073/2005

Reg1441/2005

Reg365/2010


stuffs


EnterobacteriacaeISO 21528-2: 2004

Reg2073/2005

Reg1441/2005

Reg365/2010

Dec1545/70158


stuffsEnumeration of Listeria monocytogenes ISO 11290-2: 1998 / Amd 1:2004

Reg2073/2005

Reg1441/2005

Reg365/2010


stuffs


microorganisms at 30 oC by the surface plating

technique

ISO 4833-2:2013

Reg2073/2005

Reg1441/2005

Reg365/2010

577 12 Foodstuffs Yeast and mould count ISO 21527-1:2008Reg2073/2005

Reg1441/2005578 13 Blood serum Enzootic leucosis antibodies detection Agar gel immunodiffusion

579 14 Milk Mastitis test Culture & antibiogram






Bovine leucosis


Honeybee health




broodVarroa destructor infestation


characterization / OIE Terrestrial Manual

2014 2.2.7



581 16 Apis mellifera honey bees Tropilaelaps sp. infestationMacroscopic & microscopic examination and


2014 2.2.6



582 17 Apis mellifera honey bees Average Nosema sp. spore count per sampleSpore counts in a haemacytometer / OIE

Terrestrial Manual 2014 2.2.4

MD

4424/137557/11.11.2013583 18 Apis mellifera honey bees Detection of Malpighamoeba mellificae Microscopy

584 19 Honey bees, brood Detection of Melissococcus plutonius (EFB)Culture-microscopy / OIE Terrestrial Manual

2014 Chapter 2.2.3


Health Code , Article 9.3.

585 20 Honey bees, brood Detection of Paenibacillus larvae (AFB)Culture-microscopy / OIE Terrestrial Manual

2014 Chapter 2.2.2



586 21 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy

(Beebook, coloss)

587 22 Brood Detection of Aspergillus flavusMacroscopic observation & microscopy

(Beebook, coloss)

588 23 Honey bees, brood, beehives Small Hive Beetle infestationMacroscopic observation & stereoscopy - OIE


589 24 Adult waspsDetection of Vespa velutina and other Vespidae

sp.

Macroscopic observation & stereoscopy

(EURL, method)

590 25 Adults, larvae Detection of Galleria mellonella Macroscopic observation & stereoscopy

(BeeBook, Coloss)

591 26 Adults, larvae Detection of Achroia grisellaMacroscopic observation & stereoscopy

(BeeBook, Coloss)


and birds



593 28 Internal organs Detection of pathogens Culture, isolation, identification


Enterobacter spp and other

enterobacteriaceae


595 30 Milk and udder secretionsDetection of Staphylococcus aureus &

coagulase negative staphylococci Culture, microscopy, biochemical testing

596 31 Milk and udder secretionsDetection of Streptococcus agalactiae,

Streptococcus uberis, Streptococcus

dysgalactiae



Detection of Enterococcus spp, Proteus spp,

Pasteurella spp, Pseudomonas spp,

Mycoplasma spp, Corynebacterium spp,

Trueperella pyogenes


598 33 Canned foods Incubation test at 30oC & 55oC APHA 1992, Compendium Methods 3rd ed





Method OE 504A-12 based on the method

‘Screening Test for Antibiotic Residues-STAR

Protocol’ of the NRL-Residues

Reg470/2009

Reg37/2010

Reg758/2010

Reg759/2010

Reg84/2012 Reg86/2012

Reg931/2011

Reg726/2004600


601

602 A.11 DVCA/DEPARTMENT: VETERINARY LABORATORY OF PATRAS603

604 15 Notara str, Patras, 26442605 tel: +30 2610 427383, fax: +30 2610 454027606 [email protected] 607

608 NATIONAL REFERENCE LABORATORY609 #610


613#

614 #615



619 1

Foods in form of liquid or

powder (Cat. 1) &

Homogeneous solid food (Cat.

2)


microorganisms at 30 oC.ISO 4833:2003 Reg2073/2005 ESYD 769-2 22/9/2019

620 2

Foods in form of liquid or

powder (Cat. 1) &

Homogeneous solid food (Cat.

2)


EnterobacteriaceaeISO 21528-2:2004 Reg2073/2005 ESYD 769-2 22/9/2019

621 3

Food except foods in form of

liquid or powder,

homogeneous solid food and

small solid food (Cat. 4).

Detection of Listeria monocytogenes ISO 11290-1:1996/ Amd. 1:2004) Reg2073/2005 ESYD 769-2 22/9/2019

622 4


liquid or powder,


small solid food (Cat. 4)


microorganisms at 30 oCISO 4833-1:2013 Reg2073/2005 ESYD 769-2 22/9/2019

623 5


liquid or powder,


small solid food (Cat. 4)


EnterobacteriaceaeISO 21528-2 Reg2073/2005 ESYD 769-2 22/9/2019

624 6Homogeneous solid food (Cat.

2)Detection of Listeria monocytogenes ISO 11290-1:1997 DAM 1/2005 Reg2073/2005 ESYD 769-2 22/9/2019

625 7 Milk Determination of alkaline phosphate (ALP)Fluorimetric method (ISO/DIS 11816-1, IDF

155-1 Part 1 & 2)Reg1664/2006 ESYD 769-2 22/9/2019

626

627 NON-ACCREDITED TESTS PERFORMED

628


630 8Milk, fisheries, meat, honey,

eggs

Determination of chloramphenicol content

(screening)ELISA Dec2002/657

631 9 Milk Somatic cell count ISO 13366-1:2008 Reg1664/2006

632 10 Water samplesEnumeration of fecal coliforms, enterococcus,

pseudomonasMembrane Filtration MD38295/2007

633 11 Milk products Yeast and mould count ISO 6611:2004 PD267/2002634 12 Cheese Determination of alkaline phosphate (ALP) ISO/DIS 11816-2 IDF 155-2 Καν1664/2006

Milk and milk products (alkaline phosphatase activity) Legal basis: Ministerial Desicion 3865/128713/22.10.2013, Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)



Residues of substance group A6 (chloramphenicol) of Annex I of Directive






635

13


liquid or powder,


small solid food

Detection Salmonella spp ISO/DIS 6579:2002 Reg2073/2005

636 14


liquid or powder,


small solid food

Enumeration of Escherichia coli ISO/DIS 16649-2:2001 Reg2073/2005

637 15 Homogeneous solid food Detection Salmonella spp ISO/DIS 6579:2002 Reg2073/2005

638 16 Homogeneous solid food Enumeration of Escherichia coli ISO/DIS 16649-2:2001 Reg2073/2005

639 17 Foodsuffs of animal origin Enumeration of c.p. Staphylococci ISO 6888-2:1999 Reg2073/2005

640 18 Bivalve molluscs Enumeration of Escherichia coli ISO 16649-3:2005 Reg2073/2005

64119

Blood serum of small

ruminantsDetection of antibodies against B. melitensis

Rose Bengal Test (R.B.) according to

Directive 64/432/EEC (and its updates)Dir64/432

642


643

644 A.12 DVCA/DEPARTMENT: VETERINARY LABORATORY OF RHODES645

646 4th Km Kallithea Avenue, P.C. 85100, Rhodes, Greece647 tel: +30 22410 65444, fax: +30 22410 67193648 [email protected]

650 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:651 #652 #653 #654 #655 #656 #657




stuffs (including carcasses of

cattle, sheep, goats, pigs)

Detection of Salmonella spp ISO 6579:2002 / Cor.1:2004 Reg2073/2005 ESYD 475-3 16/11/2020


stuffs


staphylococcusISO 6888-2:1999/ Amd1: 2003 Reg2073/2005 ESYD 475-3 16/11/2020

663 3

Food and Animal feeding




microorganisms at 30 oC by the pour plate

technique

ISO 4833-1:2013 Reg2073/2005 ESYD 475-3 16/11/2020


stuffs

Enumeration of b- glucuronidase- positive

Escherichia coli in agarISO 16649-2:2001 Reg2073/2005 ESYD 475-3 16/11/2020





EnterobacteriacaeISO 21528-2: 2004 Reg2073/2005 ESYD 475-3 16/11/2020


stuffsDetection of Listeria monocytogenes ISO 11290-1: 1996 / Amd 1:2004 Reg2073/2005 ESYD 475-3 16/11/2020


stuffsEnumeration of Listeria monocytogenes ISO 11290-2: 1998 / Amd 1:2004 Reg2073/2005 ESYD 475-3 16/11/2020


stuffs


microorganisms at 30 oC by the surface plating

technique

ISO 4833-2:2013 Reg2073/2005 ESYD 475-3 16/11/2020

669 9Animal faeces &



Detection of Salmonella spp ISO 6579: 2002 /Αmd.1: 2007 Reg2160/2003 ESYD 475-3 17/11/2020





Method OE 504A-12 based on the method

‘Screening Test for Antibiotic Residues-STAR

Protocol’ of the NRL-Residues

Reg37/2010 ESYD 475-3 17/11/2020

671 11 Pork meat Detection of parasite Τrichinella spp.

Magnetic stirrer method for pooled - sample

digestion (CRL Protocol according to

European Union Commission Regulation No.

1375/2015) ISO 18743:2015

Reg1375/2015 ESYD 475-3 17/11/2020

672 12 Blood serum of ruminantsDetection of antibodies against B. abortus/ B.

melitensis/ B. suis/ B. ovis


Directive 64/432/EEC (and its updates)Dir64/432 ESYD 475-3 17/11/2020

673









Bovine leucosis

Parasites (Trichinella, Echinococcus, Anisakis)


Animal by-products




675


677 13 Blood serum of ruminantsDetection of antibodies against B. abortus/ B.

melitensis/ B. suis/ B. ovis Complement Fixation Test (CFT) Dir64/432

678 14 Milk and milk products Count of yeast and moulds at 25 οC ISO 6611:2004 Reg2073/2005

679 15 Milk and udder secretions Mastitis test Whiteside test/ CMT

680 16 Milk and udder secretions Bacterial isolation, AntibiogrammaAntimicrobial Susceptibility–Agar Disk


of CLSI:Μ2, CLSI:M100, Eucast

CLSI M2 M100 EUCAST

681 17 Internal organs Bacterial detection (Gram+, Gram-) Staining, microscopy, culture, isolation


and birds



683 19 Bovine blood serum Enzootic leucosis antibodies detection ELISAOIE Terrestrial Manual

2009 Chapter 2.04.10

684 20 Bovine blood serum Enzootic leucosis antibodies detectionAgar gel immunodiffusion (AGID)


2009 Chapter 2.04.10

685 21 Bovine blood serum Detection of antibodies against Brucella sppELISA (Βrucellosis Antibody Test Kit) for

bovines OIE Terrestrial Manual,2009

Chapter3.2.1

OIE Terrestrial Manual,

2009 Chapter3.2.1

686


687

688

689

690

691 B.1 DVCTh/DEPARTMENT OF VIRAL, RICKETTSIAL DISEASES AND TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES692

693 80, 26th Oktovriou Street, 546 27 Thessaloniki694 tel:+30 210 566066, fax:+30 210 552023695 [email protected]

697 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:698 #699 #700



704 1

Nervous tissue from the obex

area of brainstem of bovines

and small ruminants (sheep

and goats)

Immuno-enzymatic Elisa test (sandwich

format) for the detection of the abnormal,

resistant to proteinase prion protein in

samples from the Central Nervous System of

infected animals

Rapid test TeSeE (BIORAD) Note:

Purification, concentration and solubilisation

of the PrPres in the samples must precede

Reg.999/2001 &

Reg.270/2002ESYD 844-2 21/11/2020

705 2 Bovine blood serumDetection of antibodies against Enzootic

Bovine Leucosis VirusELISA immunosorbent assay (indirect)

Council Directive

64/432/EEC, Ministerial

Decision

286861/31.12.2003, OIE

Manual of Diagnostic Tests

and Vaccines for Terrestrial

Animals 2012

ESYD 844-2 21/11/2020

706



710 3 Bovine milkDetection of antibodies against Enzootic

Bovine Leucosis VirusELISA immunosorbent assay (indirect)

Council Directive


Decision

286861/31.12.2003, OIE



Animals 2012

711 4Bovine blood serum & bovine

milk

Detection of antibodies against Enzootic

Bovine Leucosis VirusAgar gel immunodiffusion test

Council Directive


Decision

286861/31.12.2003, OIE



Animals 2012

712 5Bovine blood serum, plasma &

milk

Detection of antibodies against IBR-IPV-IPB

virus ELISA O.I.E. / 4th ed.,2000

713 6Bovine blood serum, plasma &

milkDetection of antibodies against BVD-MD virus ELISA (indirect) O.I.E. / 4th ed.,2000

714 7Bovine blood serum, plasma

or whole blood Detection of BVD-MD virus antigen ELISA O.I.E. / 4th ed.,2000

715 8 Bovine blood serumDetection of antibodies against Bovine

Repiratory Syncytial Virus ELISA

716 9 Sheep & goat blood serumDetection of antibodies against Maedi Visna –

CAE viruses ELISA O.I.E. / 4th ed.,2000

717 10 Blood serum from ruminantsDetection of antibodies against Chlamydia

abortus (chlamydial abortions in sheep & goat) ELISA (indirect) O.I.E. / 4th ed.,2000

718 11Sheep & goat placental

cotyledonsDetection of Chlamydia trachomatis antigen Clearview Chlamydia MF

719 12Blood serum, plasma & milk

from ruminants


burnetii (Q Fever) ELISA O.I.E. / 4th ed.,2000

720

DIRECTORATE OF VETERINARY CENTER OF THESSALONIKI (DVCTh)

Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Decision 286861/31.12.2003, Circular document no 218465/17.03.2004

Bovine Spongiform Encephalopathy & Small ruminants Transmissible Legal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)


tel:+30 210 566066, fax:+30 210 552023


721

722 B.2 DVCTh/DEPARTMENT OF FOOD MICROBIOLOGY, BIOCHEMICAL & PHYSICOCHEMICAL FOOD CONTROL, RESIDUES AND MARINE BIOTOXINS & TOXINS IN OTHER WATERS723

724 80, 26th Oktovriou Street, & 3Α, LIMNOY street, 546 27 Thessaloniki725 τηλ:2310 552928, 2310 566056, 2310 544925726 Fax: 2310 566581, 2310 544927, 2310 566055727 [email protected] ,[email protected] ,[email protected]





742 1 Shellfish (bivalve molluscs)

Analysis of lipophilic toxins in shellfish by

mouse bioassay. Qualitative determination of

okadaic acid, dinophysistoxins, pectenotoxins,

yessotoxins and azaspiracids. Detectable

lipophlilic toxins concentration of at least 160

µg okadaic acid equivalents/kg and upwards

Test method (ΔΜΤ.01) for the analysis of

lipophilic toxicity in shellfish by mouse

bioassay, based on the standard method of

Yasumoto et al. (1978) as recommended by

the European Union Reference Laboratory of

Marine Biotoxins (AESAN, Campus

Universitário de Vigo, As Lagoas-Marcosende,

Vigo, Spain)

Reg.2074/2005

Reg.15/2011ESYD 493-3 22/12/2020

743 2 Shellfish (bivalve molluscs)

Analysis of PSP toxicity in shellfish by mouse

bioassay. Range: at least 400 µg STX diHCl

equivalents/kg and upwards

Test method (ΔΜΤ.02) for the analysis of PSP

toxicity in shellfish by mouse bioassay, based

on the AOAC official method 959.08

Reg.2074/2005 ESYD 493-3 22/12/2020

744 3 Shellfish and finfish

Determination of domoic acid concentration

(ASP toxins). Range:≥1.0 mg of domoic acid

equivalents/kg and upwards.

Internal method (ΔΜΤ.03) validated

according to the requirements of the

Decision 2002/657/EC by the European

Union Reference Laboratory of Marine

Biotoxins (AESAN, Campus Universitário de

Vigo, As Lagoas-Marcosende, Vigo, Spain) by

use of High Performance Liquid

Chromatography with Ultraviolet Detection

by Diode Array Detector (HPLC-UV-DAD)

Reg.2074/2005 ESYD 493-3 22/12/2020

745 4 Animal origin foodstuffs Determination of cadmium (Cd) content OE 504 A-1 In-house method using Graphite

Furnace Atomic Absorption Spectroscopy

Reg.333/2007, 836/2011,

582/2016, 1881/2006,

629/2008, 420/2011,

488/2014, 1005/2015

ESYD 492-3 22/12/2020


Animal by-products


Residues of substance group B3c of Annex I of Directive 96/23/EC





Monitoring of marine biotoxins Legal basis: Regulation (EC) 2074/2005, Ministerial Decision 3865/12873/22.10.2013



mailto:[email protected]%20,[email protected]%20,[email protected]

746 5 Animal origin foodstuffs Determination of lead (Pb) contentOE 504 A-2 In-house method using Graphite

Furnace Atomic Absorption Spectroscopy

Reg.333/2007, 836/2011,

582/2016, 1881/2006,

629/2008, 420/2011,

488/2014, 1005/2015

ESYD 492-3 22/12/2020

747 6 Fish and fishery products Determination of mercury (Hg) content

OE 504 A-4 In-house method using Atomic

Absorption Spectroscopy FI-MH-AAS

technique

Reg.333/2007, 836/2011,

582/2016, 1881/2006,

629/2008, 420/2011,

488/2014, 1005/2015

ESYD 492-3 22/12/2020

748 7 Fish and fishery products Determination of HistamineOE 504 A-3 In-house method based on the

Reference method of Regulation (EC)

2073/2005 by HPLC-DAD

Reg.2073/2005,

1441/2007, 1019/2013 ESYD 492-3 22/12/2020

749 8

Fish and fish products. Milk

and milk products. Meat and

meat products, animal feeding

stuff, eggs and egg products

Detection of Salmonella spp.

(Salmonella typhi and paratyphi - not included)ISO 6579:2017

Reg.882/2004, 1441/2007,

854/2004, 2073/2005ESYD 844-2 21/11/2020

750 9 Molluscan bivalve shellfish Enumeration of beta-glucuronidase positive

Escherichia ColiISO 16649-3:2015 Reg.882/2004, 1441/2007,

854/2004, 2073/2005ESYD 844-2 21/11/2020

751



755 10. Shellfish Determination of lipophylic biotoxinsUltra High Performance Liquid

Chromatography -Mass Spectrometry (UPLC

MS/MS)

Reg.2074/2005

756


757

758 B.3 DVCTh/DEPARTMENT OF AVIAN & BEE DISEASES AND AQUATIC ORGANISMS' PATHOLOGY759


764 NATIONAL REFERENCE LABORATORY765 # Avian influenza766 #767

768OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:

769 #770 #771 #772 #773 #774 #775



779 1Sera samples from domestic

and wild birdsDetection of Avian Influenza antibodies ELISA immunosorbent assay

Kit IDEXX (Avian Influenza)

for chicken and turkeys. Kit

C-ELISA IDEXX (Avian

Influenza) for domestic and

wild birds

ESYD 844-2 21/11/2020


and wild birds

Detection of antibodies to different subtypes of

Avian Influenza virusHaemagglutination Inhibition test


2017 Chapter 2.3.4ESYD 844-2 21/11/2020

781 3Chicks, (live and dead), swabs,

domestic and wild birds

Avian Influenza matrix, H5, H7 and N1 genes

detection Real-Time RT-PCR

Manual VLA (2005/94/ΕC

and 2006/437/ΕC)ESYD 844-2 21/11/2020


embryonated eggs

Detection and characterization of Avian

Influenza virus


haemagglutinating factor and


characterization of Avian Influenza virus


2017 Chapter 2.3.4ESYD 844-2 21/11/2020

783 5Live chicks, avian blood and

blood serum samplesDetection of Newcastle Disease antibodies ELISA immunosorbent assay

Kit IDEXX (Newcastle

Disease) for chickenESYD 844-2 21/11/2020

784 6

Animal Faeces and



Detection of Salmonella spp. Microbiological assayISO 6579:2002/Amd1:

2007 Annex DESYD 844-2 21/11/2020

785 7Bees, brood and beehive

debris

Detection of Chronic Bee Paralysis Virus

(CBPV) by real time RT-PCR Real-Time RT-PCR

ANSES : SOP Detection and

Quantification of the

chronic bee paralysis virus

(CBPV) by real-time RT-

PCR ANA-1.MOA.1300

ESYD 844-2 21/11/2020

786 8

Bivalve molluscs (mussels,

oysters, clams, cockles,

Bearded horse mussels)

Cytological and Histological detection of

Marteilia sp parasite

Cytological and histological analysis for

bivalve molluscs according to Chapter 2.4.4

of the Manual of Diagnostic Tests for Aquatic

Animals (2016) of the World Organisation

for Animal Health (OIE)

OIE Manual of diagnostic

test for aquatic animals

IFREMER CRL for mollusk

diseases

ESYD 844-2 21/11/2020

787

Bivalve moluscs diseases

Legal basis: Directive 2005/94/EC, Presidential Decree 33/2008 (Α'59), Ministerial Desicion 4411/138539/22.12.2017 (Β'4669)

To be designated soon


Newcastle disease

West Nile fever

Honeybee health

Fish diseases

Diseases of crustaceans

Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &

Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Decision 279719/19.11.2004(Β'1760)


Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)

Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)


tel:+30 210 566051, fax:+30 210 552023




791 9 Embryonated chicken eggs Isolation of avian influenza virus Inoculation in embryonated chicken eggsOIE Terrestrial Manual

2017 Chapter 2.3.4

792 10 Embryonated chicken eggs Isolation of Newcastle disease virus Inoculation in embryonated chicken eggsOIE Terrestrial Manual

2017 Chapter 2.3.14


embryonated eggs

Detection and characterization of Newcastle

disease virus


haemagglutinating factor and


characterization of Newcastle disease virus


2017 Chapter 2.3.14


and wild birds

Detection of antibodies to Newcastle disease

virusHaemagglutination Inhibition test


2017 Chapter 2.3.14


and wild birds

Detection of antibodies to Mycoplasma

gallisepticumELISA immunosorbent assay

Kit IDEXX (Mycoplasma

gallisepticum ) for chickens

and turkeys

796 14 Chicks, (live and dead), swabs Detection of West Nile fever virus genome Real-Time RT-PCRKit LSI - TaqVet

West Nile Virus


and wild birdsDetection of antibodies to West Nile fever virus ELISA immunosorbent assay

Kit ID-Vet, OIE Terrestrial

Manual, Ch. 2.1.20, West

Nile Fever

798 16 Apis mellifera honey bees Average Nosema sp. spore count per sample Spore counts in a haemacytometer




PD

4424/137557/11.11.2013

799 17Nosema sp. spores in Apis

mellifera honey beesNosema apis & Nosema ceranae differentiation conventional PCR




PD

4424/137557/11.11.2013

800 18 Apis mellifera honey bees Detection of Malpighamoeba mellificae cysts Microscopic examination




PD

4424/137557/11.11.2013

801 19

Apis mellifera honey bees and

brood, broodcomb, hive

debris

Varroa destructor infestationMacroscopic & microscopic examination and

characterization


(May 2008) Varroosis of



broodcomb, hive debrisTropilaelaps sp. infestation


characterization


(May 2017) Infestation of

honey bees with

Tropilaelaps spp (Chapter

2.2.6.), Terrestrial Animal


803 21 Apis mellifera honey bees Acarapisosis of honey beesMacroscopic & microscopic examination and

characterization


(May 2008) Acarapisosis of

honey bees (Infestation of

honey bees with Acarapis

woodi) (Chapter 2.2.1.)

804 22Honey bees, larva/pupa,

brood comb

Detection of Paenibacillus larvae (American

Foulbrood, AFB)

Culture methods, microscopic examination of

staining smears


(May 2016) American


(Infection of honey bees

with Paenibacillus larvae)

(Chapter 2.2.2.), Terrestrial

Animal Health Code, Article

9.2.


805 23 Honey bees, broodDetection of Melissococcus plutonius (European

Foulbrood, EFB)

Culture methods, microscopic examination of

staining smears


(May 2016) European


(Infection of honey bees

with Melissococcus

plutonius) (Chapter 2.2.3.),

Terrestrial Animal Health

Code, Article 9.3.

806 24 Honey bees Honeybee virus CBPV conventional PCR

ANSES, PI: Screening for

several honeybee viruses

using PCR (ABPV, IAPV,

KBV, DWV,SBV, BQCV,

CBPV) ANA-I1.MOA.2900

807 25 Brood comb, hive debris Detection of Ascosphaera apis ( Chalkbrood) Macroscopic & microscopic examinationJournal of Apicultural

Research 52(1): (2013),

(Beebook, Coloss)

808 26 Brood comb Detection of Aspergillus flavus (Stonebrood) Macroscopic & microscopic examinationJournal of Apicultural

Research 52(1): (2013),

(Beebook, Coloss)


brood comb, beehivesSmall Hive Beetle infestation

Macroscopic & stereoscopic examination and

characterization


(July 2017) Infestation with

Aethina tumida (small hive

beetle) (Chapter

2.2.5.),Terrestrial Animal


810 28 Hornets and waspsDetection of Vespa velutina (Asian hornet and

others Vespidae sp)


characterization(EURL, method)

811 29 Adults, larvaeDetection of Galleria mellonella (Greater wax

moth)Macroscopic & stereoscopic examination

Journal of Apicultural

Research 52(1): (2013),

(Beebook, Coloss)

812 30 Adults, larvaeDetection of Achroia grisella (Lesser wax

moth)Macroscopic & stereoscopic examination

Journal of Apicultural

Research 52(1): (2013),

(Beebook, Coloss)

813 31 Tissues from bivalve molluscs

Detection of Marteillia spp genome and other

pathogens as well (Vibrio spp, ΟsHV, Bonamia

spp)

PCR / Agarose gel electrophoresis and

obsevation under a bank of UV lamps




diseases

814 32Tissues from fish, bivalve

moluscs and crustaceans Detection of Vibrio spp and Aeromonas spp

Innoculation in TSA marine matrix & blood

agar, Characterization of colonies with api

20Ε system




diseases

815 33Tissues from fish and

crustaceansParasites infestation

Microscopic examination of methylene blue

stained fresh specimens




diseases816


817

818 B.4 DVCTh/DEPARTMENT OF MICROBIOLOGY, INFECTIOUS DISEASES & BRUCELLOSIS819





830 1Blood serum samples from

ruminants


of paratuberculosis disease antibodies

Kit Paratuberculosis LSIVet screening for

ruminants


2017 Chapter 2.1.15.ESYD 844-2 21/11/2020

831 2 Blood serum samples of





Commission Regulation 535/2002/EC Reg.535/2002 ESYD 844-2 21/11/2020

832



836 3 Blood serum from ruminantsDetection of antibodies against Brucella

abortus, B. melitensisComplement Fixation Test

Reg.535/2002, MD

4224/120089/OGG2464Β/

2015, MD

3339/117339/OGG3589Β/

2016 OIE Terrestrial

Manual,2016 Chapter2.1.4

837 4 Bovine blood serumDetection of antibodies against Brucella

abortus, B. melitensis

ELISA / Kit IDEXX (Βrucellosis Antibody Test

Kit) for bovines /Kit PrioCHECK for bovines

Reg.535/2002, MD

4224/120089/OGG2464Β/



838 5 Bovine milk serumDetection of antibodies against Brucella

abortus

ELISA / Kit IDEXX (Βrucellosis Antibody Test

Kit) for bovines / Kit PrioCHECK for bovines

Reg.535/2002 MD

4224/120089/OGG2464Β/



839 6 Spleen sections/blood Detection of Bacillus anthracis Culture, microscopyOIE Terrestrial

Manual,2017 Chapter 2.1.1



nodes - Faeces

Detection of Mycobacterium paratuberculosis Microscopy OIE Terrestrial

Manual,2017 Chapter

2.1.15

Zoonoses & zoonotic agents (Brucelloses) Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)


tel:+30 210 566038, fax:+30 210 552023


841 8Sections of myocardium,

muscles, bones, internal

organs

Detection of Clostridium chauvoei, Clostridium

perfringensCulture, microscopy, biochemical testing

Clinical Veterinary

Microbiology 2nd ed Mosby

2013

842 9 Brain and liver sections Detection of Listeria monocytogenesCulture, microscopy, biochemical testing (ISO

11290-1:2017)

OIE Terrestrial


843 10Intestines, faeces, mesenteric

lymph nodes from pigsDetection of Salmonella spp

Culture, microscopy, biochemical testing (ΙSO

6579-1:2017)

OIE Terrestrial


844 11Lung tissue, synovial fluid,

milk secretionDetection of Mycoplasma spp Culture, stereoscopy

Clinical Veterinary


2013

845 12 Sheep and goat blood serumDetection of antibodies against Mycoplasma

agalactiae

ELISA (Pourquier ELISA Mycoplasma

agalactiae verification ΙDEXX)


2017, Chapter 2.7.4

846 13 Lung tissueDetection of Pasteurella

multocida/Mannheimia haemolyticaCulture, microscopy, biochemical testing

Clinical Veterinary


2013

847 14Aborted fetuses, preputial

washing

Detection of Campylobacter spp &

Tritrichomonas spp

Culture, microscopy, biochemical testing (ISO

10272-1:2017)

OIE Terrestrial


& 2.4.16

848 15 Internal organs

Detection of Escherichia coli , Corynebacterium

spp, Staphylococcus aureus,Staphylococcus spp,

Streptococcus spp


Clinical Veterinary


2013

849 16Internal organs, joints, lungs

from pigs, rabbits and

ruminants

Detection of Haemophilus spp, Βordetella spp,

Erysipelothrix sppCulture, microscopy, biochemical testing

Clinical Veterinary


2013

850 17 Internal organs Detection of Mycobacterium spp MicroscopyClinical Veterinary


2013

851 18 Cultures Antimicrobial resistance Antimicrobial Susceptibility–Agar Disk



Clinical Veterinary


2013


Enterobacter spp, Proteus spp and other

enterobacteriaceae

Culture, microscopy, biochemical testingClinical Veterinary


2013


Detection of Staphylococcus aureus, coagulase

negative staphylococci Streptococcus

agalactiae , Streptococcus uberis,

Streptococcus dysgalactiae , Streptococcus spp,

Enterococcus spp


Clinical Veterinary


2013

854 21 Milk and udder secretionsDetection of Pasteurella spp, Pseudomonas

spp, Corynebacterium spp, Trueperella

pyogenes

Culture, microscopy, biochemical testingClinical Veterinary


2013

855 22Milk and udder secretions,

internal organsDetection of yeasts - moulds Culture, microscopy

Clinical Veterinary


2013856


857

858 Β.5 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF IOANNINA859

860 Chr. Katsari 2, PC 454 44, Ioannina861 tel: +30 26510 27396, fax: +30 26510 33541862 [email protected] 863


866 #

867 #868 #

869 #

870 #

871 #

872 #

873 #

874



878 1 Food and animal feeding stuffDetection of Salmonella spp ( except S. typhi , S.

paratyphi)ISO 6579-1:2017

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

879 2 Food and animal feeding stuff Detection of Listeria monocytogenes ISO 11290-1:2017

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

880 3 Food and animal feeding stuff Enumeration of Listeria monocytogenes ISO 11290-2:2017

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

881 4 Food and animal feeding stuffEnumeration of coagulase positive

staphylococciISO 6888-2:1999

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

882 5 Food and animal feeding stuffEnumeration of β-glucoronidase positive

Escherichia coli ISO 16649-2:2001

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

883 6 Food and animal feeding stuffEnumeration of the total aerobic

microorganisms at 30 oCISO 4833-1 :2013

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

884 7 Food and animal feeding stuffDetection and enumeration of

enterobacteriaceaeISO 21528-2:2017

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

885 8

Animal faeces and samples

from the primary production

stage

Detection of Salmonella spp ( except S. typhi , S.

paratyphi)ISO 6579-1:2017

Reg2073/2005,

Reg1441/2007,

Reg365/2010,

Reg1086/2011,

Reg209/2013

ESYD488-3 16/12/2020

886 9 Animal and poultry tissues




Method OE504A-28 based on the method

“Screening Test for Antibiotic Residues

–STAR Protocol” of the NRL-Residues

Reg657/2002,

Reg37/2010, GUIDELINES

FOR THE VALIDATION OF

SCREENING METHODS (20-

1-2010)

ESYD488-3 16/12/2020

887 10

Digestion residues from

biogas plants – compost from

composting plants

Detection of Salmonella spp ( except S. typhi , S.

paratyphi)

Based on ISO 6579-1:2017 for the purpose of

Commission Regulation (EC) No 1069/2009

and implementing Regulation (EU) No

142/2011

Reg1069/2011,

Reg142/2011ESYD488-3 16/12/2020




Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &

1751/51822/24.04.2013(B'1141)Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004Legal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)








Bovine leucosis


Animal by-products





888 11

Digestion residues from

biogas plants – compost from

composting plants


Escherichia coli

Based on ISO 16649-2:2001 for the purpose

of Commission Regulation (EC) No

1069/2009 and implementing Regulation

(EU) No 142/2011

Reg1069/2011,

Reg142/2011ESYD488-3 16/12/2020

889 12Nervous tissue (obex) of

bovine, ovine and caprine

originDetection of the protein PrPRes Immuno-enzymatic technique (TeSeE BIO-

RAD rapid test) according to the Commission Reg999/2001 ESYD488-3 16/12/2020

890 13











Dir64/432/EEC,

Reg535/2002ESYD488-3 16/12/2020

891 14











Dir64/432/ΕEC,

Reg535/2002ESYD488-3 16/12/2020


bovine


Virus


Directive 64/432/EC, the Decision

2009/976/ΕΕ and the OIE Manual of

Diagnostic Tests and Vaccines for Terrestrial

Animals 2012

Dir64/432/ΕEC,

Dec2009/976/ΕUESYD488-3 16/12/2020

893 16Milk samples of domestic or

wild ruminants







Dir64/432/ΕEC,

Reg535/2002ESYD488-3 16/12/2020

894


896


898 17 Bovine blood serum sampleDetection of antibodies against Bovine Leucosis

VirusAgar gel immunodiffusion test

Dir64/432/ΕΟΚ,

Dec2009/976/ΕU, OIE



Animals 2012

899 18 Avian caeca Detection of C. jejuni ISO 10272-1 modifiedDec2013/653,

JMD1571/72152/2014

900 19 caecal samplesDetection of ESBL-, AmpC- and carbapenemase-

producing E. coli EURL-AR DTU November 2017 5th edition

Desicion 2013/652/ΕU,

Joint Ministerial Desicion

1920/88493/22.08.2017

(Β'3225)

901 20 fresh meatDetection of ESBL-, AmpC- and carbapenemase-

producing E. coli EURL-AR DTU October 2015 3rd edition

Desicion 2013/652/ΕU,

Joint Ministerial Desicion

1920/88493/22.08.2017

(Β'3225)

902 21 Bovine milk sampleDetection of antibodies against Bovine Leucosis

VirusELISA Test

Dir64/432/ΕΟΚ,

Dec2009/976/ΕU, OIE



Animals 2012903


904

905 Β.6 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF KAVALA906

907 Stavros Amygdaleona, PC: 640 12, Kavala908 tel: +30 2510 391865, fax: +30 2510 392301 909 [email protected] 910



916 #

917 #

918 #

919 #

920



924 1 Bivalve molluscsEnumeration of β-glucuronidase positive E.

Coli

ISO/TS 16649-3:2015 Most probable

number, using 5-bromo-4-chloro-3-indolyl-β-

D-glucuronide

Reg2073/2005,

Reg1441/2007ESYD529-3 29/3/2021

925 2Food except small solid

foodstuff

Detection of Salmonella spp. Horizontal

method for the detection of non-typhi, non-

paratyphι Salmonella spp.

ISO 6579:1:2017Reg2073/2005,

Reg1441/2007ESYD529-3 29/3/2021

926 3Food and Animal Feeding

stuffsDetection of Listeria monocytogenes ISO 11290-1:2017

Reg2073/2005,

Reg1441/2007ESYD529-3 29/3/2021





Method MD-07 based on “Screening Test for

Antibiotic Residues (STAR Protocol” of the

LVM/RMV/BIO/PTC/ 15.AN/17-10-2006

Protocol of Community Reference

Laboratory of AFFSA-FOUGERES 6th Edition)

Reg657/2002,

Reg37/2010, GUIDELINES

FOR THE VALIDATION OF

SCREENING METHODS (20-

1-2010

ESYD529-3 29/3/2021

928 5








the Commission Regulation 535/2002/EC



Dir64/432/EEC ESYD529-3 29/3/2021

929


931


933 6











Dir64/432/EEC

934 7 Bovine milk sample Detection of antibodies against Βrucella






Dir64/432/EEC

935 8 Bovine blood serumDetection of antibodies against Βrucella





Dir64/432/EEC

936 9 Bovine milk sample Detection of antibodies against Bovine Leucosis

VirusELISA Test

Dir64/432/ΕEC,

Dec2009/976/ΕΕ


bovine


VirusELISA

Dir64/432/ΕEC,

Dec2009/976/ΕU


brood

Detection of Varroa destructor (Varroosis)

mites and their counting


identification / OIE Terrestrial Manual 2008

2.2.7



Residues of substance group B1 of Annex I of Directive 96/23/EC Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)


Zoonoses & zoonotic agents (Brucelloses) Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)

Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004

Microbiological criteria for foodstuffs Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)




939 12 Apis mellifera honey beesDetection of Tropilaelaps spp. (tropipelalps

infestination of bees)


identification/ OIE Terrestrial Manual 2017

2.2.6



940 13 Forager bees Apis mellifera

detection of Nosema spp. (spores) (qualitative

determination) and their count

(quantification)

Microscopic examination, spore count on

hemocytometer and determination of mean

spore number per bee / OIE Terrestrial

Manual 2013 2.2.4

MD

4424/137557/11.11.2013

941 14 Apis mellifera honey bees Detection of Malpighamoeba mellificaeMicroscopic examination of malpighian

tubules/OIE Terrestrial Manual 2013 2.2.4

942 15 Honey bees, broodDetection of Melissococcus plutonius (European

Foul Brood, EFB)

Culture-microscopy / OIE Terrestrial Manual

2016 Chapter 2.2.3



943 16 Honey bees, broodDetection of Paenibacillus larvae (American

Foul Brood, AFB)

Culture-microscopy / OIE Terrestrial Manual

2016 Chapter 2.2.2



944 17 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy

(Beebook Vollume II, coloss)

945 18 Brood Detection of Aspergillus flavusMacroscopic observation & microscopy

(Beebook Vollume II, coloss)

946 19 Honey bees, brood, beehives Small Hive Beetle infestationMacroscopic observation & stereoscopy - OIE




947 20 Adult waspsDetection of Vespa velutina and other Vespidae

sp.


(EURL, method)

948 21 Adults, larvaeDetection of Galleria mellonella and Achroia

grisella ( Greater and Lesser max moths )


(BeeBook Vollume II, Coloss)

949 22 Bees of Apis melliferaDetection of Acarapis woodi mites

(Acarapisosis)

Mιcroscopic observation & identification OIE

Terrestrial Manual 2008 2.2.1.




stuffsEnumeration of Listeria monocytogenes ISO 11290-2:2017 Reg 2073/2005


stuffs


staphylococciISO 6888-2:1999 Reg 2073/2005


stuffs


Escherichia coliISO 16649-2:2001 Reg 2073/2005


stuffs

Detection and Enumeration of

EnterobacteriaceaeISO 21528-1:2017 & ISO 21528-2:2017 Reg 2073/2005

954 27 Food and Animal Feeding

stuffs


microorganisms at 30 oCISO 4833-1:2013 Reg 2073/2005

955 28 Dead animals and tissuesNecrotomic, parasitic, microbiological

examinations

Necropsy and sampling for further

microscopic or microbiological examination

956


957

958 Β.7 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF SERRES959

960 Terma Omonoias, PC 621 10, Serres 961 tel: +30 23210 35411, fax: +30 23210 35410 962 [email protected] 963

964NATIONAL REFERENCE LABORATORY

965 #

966



970 1 Bovine urine Determination of anabolic steroids: 17α-

trenbolone, methyltestosterone

Internal method OMT.001 by liquid

chromatography-tandem mass spectrometry.

(LC-MS/MS) based on the method of the Eur.

Community Refence lab (RIVM, Bilthoven,

The Netherlands)

Dec657/2002 ESYD 482 19/11/2020

971 2 Bovine urine

Determination of anabolic steroids:

diethylstilbestrol, dienestrol, hexestrol, β-

estradiol, ethynylestradiol, α-zearalanol, β-

zearalanol, stanozolol, 16β-hydroxystanozolol,

α-nortestosterone

Internal method OMT.003 by liquid




The Netherlands)

Dec657/2002 ESYD 482 19/11/2020

972 3 Βovine muscle tissueDetermination of anabolic steroids: 17β-

trenbolone, methyltestosterone

Internal method OMT 002 by liquid




The Netherlands)

Dec657/2002 ESYD 482 19/11/2020

973 4 FatDetermination of organochlorine pesticides:

cis-chlordane, trans-chlordane, aldrin, dieldrin

Internal method OMT 004 by gas


(GC-MS/MS) based on the EPA methods of

pesticides

Dec657/2002 ESYD 482 19/11/2020

974


976


978 5Caprine,Ovine and porcine

urine

Determination of 17α-trenbolone,

methyltestosterone

Liquid chromatography-tandem mass

spectrometry (LC-MS/MS)Dec657/2002 Validated

979 6Caprine,Ovine and porcine

urine

Determination of hormones

(stilbenes)diethylstilbestrol, dienestrol,

hexestrol



980 7 Bovine urineDetermination of α-zearalenol, β-zearalenol,

zearalenone, α-boldenone, β-boldenone, β-

testosterone



981 8Muscle tissue of

caprines,sheep,fisheries,

poultry, rabbits, game animals

Determination of 17β-trenbolone,

methyltestosterone



Residues of substance groups A1, Α3, Α4, Α6 (dapsone), Β2f (carbadox,

olaquindox), Β3a of Annex I of Directive 96/23/ECLegal basis: Desicion 98/563/ΕC



982 9

Muscle tissue of fisheries,

bovines, ovines, caprines,

poultry, rabbits, wild and

game animals

Determination of diethylstilbestrolLiquid chromatography-tandem mass


983 10Blood samples from bovines,

ovines, caprines, avians,

porcines

Determination of lactones: α-zearalanol, β-

zearalanol



984 11




game animals

Determination of lactones:zearalenone, α-

zearalanol (zeranol), α- zearalenol, β-

zearalanol (taleranol), β- zearalenol



985 12 Ovine, caprine and porcine

urine

Determination of hormones:β-estradiol,

ethynylestradiol, stanozolol, 16β-OH-

hydroxystanozolol, α-nortestosterone, α-

boldenone, β-boldenone, β- testosterone



986 13

Liver and muscle tissue

(fisheries, bovines, ovines,

caprines, poultry, rabbits, wild

and game animals)

Determination of corticosteroids:

dexamethasone, flumethasone, triamcinolone

acetonide



987 14 Fat

Determination of gestagens:

medroxyprogesterone acetate, megestrol

acetate, melengestrol acetate, chlormadinone

acetate





porcines

Determination of ethynylestradiolLiquid chromatography-tandem mass


989 16




game animals

Determination of ethynylestradiolLiquid chromatography-tandem mass




porcines

Determination of methyltestosterone, α-

trenbolone, β-trenbolone, α-boldenone, β-

boldenone, α-nortestosterone, β-

nortestosterone



991 18




game animals

Determination of α-boldenone, β-boldenone, α-

nortestosterone, β-nortestosterone



992 19Bovine, ovine and porcine

urine

Determination of lactones:zearalenone, α-

zearalanol (zeranol), α- zearalenol, β-

zearalanol (taleranol), β- zearalenol





porcines

Determination of α-estradiol, β-estradiolLiquid chromatography-tandem mass


994 21




game animals

Determination of β-estradiolLiquid chromatography-tandem mass


995 22 FatDetermination of HCB, Heptachlor, Endosulfan,

Endrin, p,p`DDE, p,p`DDD- o,p`DDT

Gas chromatography-tandem mass

spectrometry (GC-MS/MS)Dec657/2002 Validated

996 23 FatDetermination of PCB_28, PCB_52, PCB_101,

PCB_118, PCB_180


spectrometry (GC-MS/MS)Dec657/2002 Validated

997 24

Muscle tissue of bovines,

ovines, caprines, poultry,

rabbits, wild and game

animals

Determination of carbadox and metabolites

CBX carbadox, QCA quinoxaline-2-carboxylic

acid, mQCA methyl-3-quinoxaline-2-carboxylic

acid



998 25

Muscle tissue of bovines,

ovines, caprines, poultry,

rabbits, wild and game

animals

Determination of dapsoneLiquid chromatography-tandem mass


999 26Milk from bovines, ovines,

caprinesDetermination of dapsone



1000 27




game animals

Determination of hexestrol, dienestrolLiquid chromatography-tandem mass

spectrometry (LC-MS/MS)


1001 28 Milk

Determination of α-HCH, HCB, Lindane,

Heptachlor, Heptachlor epoxide, aldrin, α-

Endosulfan, β-Endosulfan, cis-Chlordane, trans-

Chlordane, p,p’-DDE, o,p’-DDT, p,p’-DDD, p,p’-

DDT


spectrometry (GC-MS/MS)SANTE/11813/2017 Validated

1002 29 Live bivalve molluscs (mussels) Determination of polychlorionated biphenylsGas chromatography-tandem mass

spectrometry (GC-MS/MS)SANTE/11813/2017 Validated

1003 30 FatDetermination of: α-HCH, β-HCH, Lindane,

Heptachlor epoxide, β-Endosulfan, p,p’-DDT


spectrometry (GC-MS/MS)

1004 31 FatDetermination of Polychlorinated biphenyls:

PCB138, PCB153



1005 32 Honey,eggs, fisheriesDetermination of Polychlorinated biphenyls:

PCB28, PCB52, PCB101, PCB118, PCB138,

PCB153, PCB180



1006 33 Honey,eggs, fisheries

Determination of: α-HCH, HCB, Lindane, β-HCH,

Heptachlor, Heptachlor epoxide, Aldrin,

Dieldrin, Endrin, α-Endosulfan, β-Endosulfan,

cis-Chlordane, trans-Chlordane, p,p’-DDE, o,p’-

DDT, p,p’-DDD, p,p’-DDT



1007 34 MilkDetermination of: PCB28, PCB52, PCB101,

PCB118, PCB138, PCB153, PCB180



1008 35 Blood smears of livestockDetection of blood parasites Babesia spp.

Αnaplasma spp.

Microscopic examination of blood stained

with Giemsa-May Grunwald stainO.I.E. Terrestrial Manual

1009 36Faecal smears of livestock,

poultry, game birds and

companion animals

Detection of Cryptosporidium spp oocysts Acid-fast Ziehl-Neelsen stain


(May 2016)

Cryptosporidiosis (Chapter

2.9.4.)



nodes - Faeces

Detection of Mycobacterium paratuberculosis Ziehl-Neelsen stain, microscopy OIE Terrestrial

Manual,2017 Chapter

2.1.15

1011 38affected tissues, internal

organs,milk,

Microbiological searching for bacterial

pathogens Pasteurella multocida/Mannheimia

haemolytica, Staphylococcus spp.,

Streptococcus spp., Pseudomonas spp.,

Escherichia coli, Klebsiella spp., Enterobacter

spp.

Culture, isolation, identification




WOLFE, 1994.

1012 39 small ruminans milkContagious agalactia in small ruminants

(detection of Mycobplasma agalactiae)Culture, stereoscopy, isolation, identification


2013 Chapter 2.7.4.1013 40 Faeces Detection of oocysts in parasitic infections Teleman sedimentation method

1014 41 Lung tissue,milk Detection of Mycoplasma spp Culture, microscopyOIE Terrestrial Manual

2013 Chapter 2.7.4.

1015 42 Cultures AntibiogramsAntimicrobial Susceptibility–Agar Disk



CLSI M2 M100 EUCAST


broodVarroa destructor infestation



2014 2.2.7


2008 Chapter 2.2.7.

1017 44 Apis mellifera honey beesDetection of Nosema spp. (spores) (qualitative

determination)

Microscopic examination and detection of

spores


2013 Chapter 2.2.4.

1018 45 Apis mellifera honey bees AcarapisosisMacroscopic & microscopic examination and

characterization


2014 Chapter 2.2.1.

1019 46 Honey bees, brood Detection of Melissococcus plutonius (EFB) Culture-microscopy OIE Terrestrial Manual

2016 Chapter 2.2.3.

1020 47 Honey bees, brood Detection of Paenibacillus larvae (AFB) Culture-microscopy / OIE Terrestrial O.I.E. Terrestrial Manual

2014 Chapter 2.2.21021 48 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy Beebook, Coloss

1022 49 Brood Detection of Aspergillus flavus Macroscopic observation & microscopy Beebook, Coloss

1023 50 Adults, larvae Detection of Galleria mellonella ,Achroia grisella Macroscopic observation & stereoscopy Beebook, Coloss

1024


1025

1026 Β.8 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF LARISSA1027

1028 6th km of Larissa-Trikala highway, PC 411 10, Larissa1029 tel:+30 2410 617980, fax: +30 2410 6179821030 [email protected]


1033 #

1034 #1035 #1036 #

1037 #

1038


1041 #

1042 # Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.20041043



1047 1

Nervous tissue (obex) of


originDetection of the protein PrPRes

Immuno-enzymatic technique ΕLΙSΑ (TeSeE

BIO- RAD) according to the Commission

Regulation 999/2001/EC

Reg999/2001 ESYD 834-2 17/9/2020

1048 2

Nervous tissue (obex) of


originDetection of the protein PrPRes

Immunoblot test (Confirmatory Western blot

BIO-RAD) according to the Commission

Regulation 999/2001/EC

Reg999/2001 ESYD 834-2 17/9/2020

1049 3Nervous tissue (obex) of ovine

and caprine origin

Discriminatory test for strain typing of

transmissible spongiform encefalopathies in

small ruminants

Discriminatory test (Discriminatory CEA

METHOD BIO-RAD) according to the

Commission Regulation 36/2005/EC and the

«Discriminatory Testing Handbook of AHVLA

EU community reference laboratory for TSE

as amended

Reg36/2005 ESYD 834-2 17/9/2020

1050 4











Reg535/2002 ESYD 834-2 17/9/2020

1051 5











Reg535/2002 ESYD 834-2 17/9/2020

1052 6 Milk Detection of Campylobacter spp. ΕLΟΤ ΕΝ ISO 10272-1:2006 Reg854/2004 ESYD 641-2 1/12/2017

1053 7Milk and milk products, meat

and meat productsDetection of Salmonella spp. ΕLΟΤ ΕΝ ISO 6579:2002 Reg2073/2005 ESYD 641-2 1/12/2017

1054 8Milk and milk products, meat

and meat productsDetection of Listeria monocytogenes ΕLΟΤ ΕΝ ISO 11290-1:1996/Amd 1:2004 Reg2073/2005 ESYD 641-2 1/12/2017

1055 9 Raw cow milk Enumeration of somatic cellsMicroscopic method (Reference method) ISO

13366-1/IDF 148-1:2008Reg853/2004 ESYD 641-2 1/12/2017

1056



Cambylobacter


Total water content of poultry meat


Legal basis: Regulation (ΕC) 999/2001, Regulation (ΕU) 1148/2014

Legal basis: Ministerial Desicion 3865/128713/22.10.2013


Legal basis: Regulation (EC) 543/2008, Ministerial Decision 327688/12.12.2005


Honeybee health




tel:+30 2410 617980, fax: +30 2410 617982



1058


1060 10 MilkEnumeration of the total aerobic

microorganisms at 30oC ISO 4833-1:2013 Reg853/2004

1061 11Ovine nervous tissue

Ovine blood samples

Genotyping analysis of polymorphisms in the

PrP protein gene codons 136, 141, 154 and 171

to determine resistance to Scrapie Disease.

Multiplex RT-PCR Melting Curve Genotyping

using specific fluorescent

probesReg999/2001

1062 12 Bovine milk samples Antibody detection against Brucella spp.





Καν535/2003

1063 13 Bovine blood serum samples Antibody detection against Brucella spp. Indirect ELISA Καν535/2003

1064 14 Bovine blood serum, milk Antibody detection against Enzootic Bovine

Leucosis

BLV gp51 Ab Test, Screening format, Kit

Svanova & BLV gp51 Ab Test, Confirmation

format, Kit Svanova.

Direct.64/432/ΕΟΚ, Dis.

2009/976/ΕΕ

1065 15 Apis mellifera honey bees and

brood

Varroa destructor infestation. Macroscopic-

microscopic examination and characterization.

Macroscopic-microscopic examination and

characterization of Varroa destructor.


2008 Chapter 2.2.7.

1066 16 Apis mellifera honey bees Nosema diagnosisAverage Nosema sp spore count per sample

in a haemacytometer.


2013 Chapter 2.2.4.

1067 17Control of REV-1 & RB-51

Vaccines

Detection of Βrucella melitensis REV-1strain

& Brucella Abortus RB-51 strainCulture Isolation Identification Enumeration.

Manual of diagnostic Test

and Vaccines for

TERRESTRIAL Animals

1068 18Aborted tissue of domestic

animals

Detection of Βrucella spp, Cambylobacter spp,

Salmonella spp, Culture Isolation Identification

Manual of diagnostic Test

and Vaccines for

TERRESTRIAL Animals

2013 Chapter 2.4.3

and2.7.2 Techniques for

Brucellosis laboratory G.G.

ALTON/L.M.JONES

R.D.ANGUS/J.M.VERGER.ΕΝ

ΙSO 10272-1:2017 EN ISO

6579:2002

1069 19Blood smears of domestic

animalsDetection of all parasites

Microscopic examination - Giemsa stain-May

Grunwald stain




1070 20Faecal smears of domestic

animals Detection Cryptosporidium spp oocysts

Microscopic examination - Modified Ziehl-

Neelsen


2016 Chapter 2.9.4.

1071 21Lymphnotes and intestinal

smaears od domestic animals

Detection of Mycobacterium Avium subsp.

Paratuberculosis

Microscopic examination - Modified Ziehl-

Neelsen


2014 Chapter 2.1.15.

1072 22Animal Tissue and milk from

domestic animals

Isolation of Pasteurella

multocida/Mannheimia haemolytica,

Staphylococcus spp., Streptococcus spp.,

Pseudomonas spp., Escherichia coli, Klebsiella

spp., Enterobacter spp. Salmonella spp,

Clostridium Spp

Culture, Isolation Identification




WOLFE, 1994.

1073 23 Milk, Lung tissue Detection of Mycoplasma spp. Culture, Stereoscopy, IdentificationOIE Terrestrial Manual

2013 Chapter 2.7.4.

1074 24 Culture of isolated bacteria Disk-Antibiogram (CLSI M2 M100 EUCAST). CLSI M2 M100 EUCAST

1075 25 FoodEnumeration of the total aerobic

microorganisms at 30oCISO4883-1:2013 Καν2073/2005

1076 26 FoodEnumeration of coagulase positive

staphylococciISO 6888-1:1999 Καν2073/2005

1077 27 FoodEnumeration of β-glucoronidase positive

Escherichia coliISO 16649-2:2001 Καν2073/2005

1078 28 Poultry meat Water content

Determination of nitrogen content (ISO

937:1978) Determination of water content

(ISO 1442:1997)

Reg1538/1991

1079 29 Raw milk Determination of albendazole sulfoxideInternal method (Journal of AOAC

International, Vol. 79, No. 6, 1996)Reg37/2010, Reg470/2009

1080


1081

1082 Β.9 DVCTh/BIOLOGICAL PRODUCTS, DIAGNOSTIC PATHOLOGY & PARASITOLOGY OFFICE1083



1090


1093

Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used

Documentation Certificate number Valid until

1094 1

Bovine tissues (Lymph nodes:

retropharyngeal, bronchial,

mediastinal and portal. Lung,

liver, spleen and the surfaces

of body cavities-diaphragm)

Histological method for tubercle's detection Haematoxyline - Eosin staining for tubercle's

detection

Council Directive

64/432/EEC, PD 308/2000,

MD 30/3430/14.01.15

ESYD 844-2 21/11/2020

1095



1099 2Tissues of goats ,sheep and

petsHistological method for tubercle's detection

Haematoxyline - Eosin staining for tubercle's

detection

Council Directive

64/432/EEC, PD 308/2000,

MD 30/3430/14.01.15,

Wangoo et al (2005)

J.Comp.Path.,133,223-234

Validated

1100 3 Lungs of goats and sheepHistological method for detection of maedi

pneumonia Haematoxyline - Eosin staining

Pathology of Domestic

Animals, Jubb, Kennedy &

Palmer's, Elsevier

Saunders, 2007

1101 4 Lungs of goats and sheep Histological method for detection of parasitesHaematoxyline - Eosin staining for detection

of parasitic bronchopneumonia



Palmer's, Elsevier

Saunders, 2007

1102 5 Lungs of goats and sheepHistological method for detection of

Mannhemia spp pneumonia Haematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1103 6 Lungs and pleura of goats Histological method for detection of infectious

pleuropneumoniaHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1104 7 Brain of goats and sheep Histological method for visna -maedi detection Haematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1105 8 Brain of goats and sheep Histological method for Listeria detection Haematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1106 9 Brain of goats and sheepHistological method for the detection of

embolic granulomatous meningoencephalitisHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1107 10 Brain of goats and sheepHistological method for the detection of

parasitic encephalitisHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1108 11 Brain of goats and sheepHistological method for the detection of VitA

deficiencyHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

Zoonoses & zoonotic agents (Bovine Tuberculosis) Legal basis: Ministerial Decision 30/3430/14.01.2015(B'171)


tel:+30 210 566063, fax:+30 210 552023


1109 12Mucosa of nose cavities of

sheep

Histological method for the detection of

endemic intramucosal neoplasia of the noseHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1110 13 Lips skin of the sheepHistological method for the detection of

contagious ecthyma Haematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1111 14 Skin of goats and sheepHistological method for the detection of

sarcoptic,psoroptic and chorioptic scabiesHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1112 15 Mammary gland of sheepHistological method for the detection of mixed

infection by Maedi-Corynebacterium sppHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1113 16 Mammary gland of cowHistological method for the detection of

gangrenous mastitisHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1114 17 Tissues of productive animalsHistological method for the detection of

neoplasiasHaematoxyline - Eosin staining

Tumors in Domestic

Animals 4th ed.Meuten

Editor Blackwell ,2002

1115 18 Tissues of rabbit Histological method for the detection of

myxomatosisHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1116 19 Meat of slaughetered bovineHistological method for the detection of

eosinophilic myiositisHaematoxyline - Eosin staining



Palmer's, Elsevier

Saunders, 2007

1117 20 Poultry LiverHistological method for the detection of

hepatitis with inclusion bodies (adenovirus)

and mycotoxicosis

Haematoxyline - Eosin stainingDiseases of Poultry 10th

Ed.B.W.Calnek,Mosby-

Wolfe,1997

1118 21 Tissues of laying chickens Histological method for the detection of Marek

diseaseHaematoxyline - Eosin staining

Diseases of Poultry 10th

Ed.B.W.Calnek,Mosby-

Wolfe,1997

1119 22Tissues of laboratory animals

(white mice)

Istological examination within the framework

of the European and Greek legislationHaematoxyline - Eosin staining

PD56/2013, Reg15/2011,

Digestive System,

Monographs In Pathology

of Laboratory Animals,Ed.

T.C. Jones ,U.Mohr,

R.D.Hunt, Springer

Verlag,1985

1120 23Tissues of laboratory animals

(white mice)Istological examination for neoplasias Haematoxyline - Eosin staining

Pathology of Neoplasia and

Preneoplasia in Rodents

EULEP Color Atlas, Bannash

and

Gossner,Schattauer,1994


Documents

updated on 31.01.2018 NETWORK OF OFFICIAL …...updated on 31.01.2018 a.1 (54-104) a.2 (107-149) a.3 (152-249) a.4 (252-302) a.5 dvca/department of food hygiene (305-356) a.6 (359-403)