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1
Viruses & Bacteria
Name: ________________
Hour: ____
2
Vocabulary and Big Ideas
Viruses Bacteria
virus
host parasite
vaccine
bacteria
cytoplasm
ribosome
flagellum
cellular respiration
binary fission
conjugation
endospore
pasteurization
decomposer
Long-term “I Can” Statements
Viruses Bacteria
I can… Date done Activity to support
I can… Date done Activity to support
…list the characteristics of viruses.
...draw and label a bacterial cell and its structures.
…explain how a virus is similar to a parasite.
…classify bacteria by its shape.
...understand how small a virus is.
…list and explain how bacteria get food, get energy, and reproduce.
…draw how a virus attaches to a host cell.
…differentiate between asexual and sexual reproduction.
…explain how viruses interact with the living world.
…understand bacteria’s role in nature.
…describe how a vaccine works.
Unit: Viruses & Bacteria Unit Completion Date:
3
How Many Viruses Fit on the Head of a Pin? Problem How small is a virus? Materials straight pin long strips of paper pencil meter stick scissors tape calculator imagination Procedure
1. Carefully, examine the head of a straight pin. Write a prediction about the number of viruses that could fit on the pinhead.
My prediction is: __________________ viruses.
2. Assume that the pinhead has a diameter of about 1 mm. If the pinhead were
enlarged 10,000 times, its diameter would measure 10 m. Create a model of a pinhead by cutting and taping together narrow strips of paper to make a strip that is 10 m long. The strip of paper represents the diameter of the enlarged pinhead.
3. Lay the 10 m strip of paper on the floor. Imagine creating a large circle that had
the strip as its diameter. The circle would be the pinhead at the enlarged size. Calculate the area of the enlarged pinhead using this formula:
A = π x radius2 = __________________ m2
4. A virus particle may measure 200 nm on each side (1 nm equals a billionth of a
meter). If the virus were enlarged 10,000 times, each side would measure 0.002 m. Cut out a square 0.002 m by 0.002 m to serve as a model for a virus. (Hint: 0.002 m = 2 mm).
5. Next, find the area in meters of one virus particle at the enlarged size. Remember that the area of a square equals side x side.
A = __________________ m2
6. Now divide the area of the pinhead that you calculated in Step 3 by the area of
one virus particle (Step 5) to find out how many viruses could fit on the pinhead.
__________________ viruses
7. Exchange your work with a partner, and check each other’s calculations.
Calculations:
d
4
How Many Viruses Fit on the Head of a Pin? CONTINUED… Conclusion
1. How does your calculation compare with the prediction you made? If the two numbers are very different, explain why your prediction may have been inaccurate.
2. What did you learn about the size of viruses by magnifying both the viruses and pinheads to 10,000 times their actual size?
3. Explain why scientists sometimes make and use enlarged models of very small things, such as viruses.
5
How Viruses Spread Vaccines are substances that help protect against diseases caused by
viruses. In this activity, you will see how vaccines affect the spread of
viral “diseases” in your classroom.
INQUIRY FOCUS Relate Evidence and Explanation
Procedure
1. Study your Vaccination Card. There are five imaginary
viruses that will circulate through the classroom. Your Vaccination Card lists the viruses and indicates which
vaccines you have received.
2. When you are handed a Virus Card, check to see if you are vaccinated for that virus.
3. Then, follow the appropriate instructions on the Virus
Card. When you receive a virus for which you are not vaccinated, you will be immune to that virus in the future.
4. Classmates who are immune to a particular virus or have already had that virus
will not accept that Virus Card from you. Keep trying to give the card to someone
until you succeed.
Think It Over
Based on the summary of how many students “caught” each virus, which viruses
were the most people vaccinated for?
If you had complete immunity for a particular virus, why did you give the Virus
Card back to the person who gave it to you?
Use the evidence you gathered in this activity to explain why vaccines are
important for preventing the spread of a viral disease.
Materials
1 Vaccination Card per student
5 Virus Cards
collection of “aprons” and “goggles”
6
7
8
Viruses
1. Viruses are considered to be nonliving. How are they similar to living organisms and how are they
different?
2. How are viruses similar to parasites?
3. In the diagram below, what is the structure and function of the part labeled “A”?
4. In the diagram below, what is the structure and function of the part labeled “B”?
5. virus
6. host
7. parasite
8. vaccine
a. an organism that lives on or in a host and causes it harm
b. a substance introduced in the body to help produce chemicals that destroy specific viruses
c. an organism that provides a source of energy for a virus or another organism
d. a tiny, nonliving particle that enters and then reproduces inside a living cell
Understanding Main Ideas
Answer the following questions.
Building Vocabulary
Match each term with its definition by writing the letter of the correct definition in the right column on the line beside the term in the left column.
9
Viruses
Viral Multiplication
Different kinds of viruses multiply at different rates. The rate at which a virus multiplies and when and how this multiplication takes place can help to identify the virus. The graph below shows the rate of growth of four groups of animal viruses, that is, viruses that infect animal cells.
To collect the data for the graph above, scientists grew viruses in the laboratory. Counting viruses is very difficult because they are so small. It’s much easier to measure how effective a virus is at killing cells. So in the graph, the y-axis of the graph shows the number of cells that have been destroyed because of viral activity. For each kind of virus, a higher number of cells killed by the virus means a higher number of virus particles.
1. Which kind of virus begins multiplying first? How soon after infection does this happen?
2. Which kind of virus begins multiplying last? How soon after infection does this happen?
3. What is similar about the four lines on the graph? What does this mean about the growth rate of the four kinds of virus groups?
4. The upper part of the line representing the growth curve of the herpesvirus is nearly horizontal. What
does this mean about the rate of multiplication of the herpesvirus from 15 to 20 hours after infection?
Read the passage and study the graph below. Then, answer the questions that follow.
10
How Quickly Can Bacteria Multiply? A bacterium can reproduce every twenty minutes. At this rate, imagine
how large a bacteria population could become in one day!
INQUIRY FOCUS Graph
Procedure
1. Your teacher will give you some beans and plastic
cups. Number the cups 1 through 8. Each bean will represent a bacterial cell.
2. Put one bean into Cup 1 to represent the first generation of bacteria. Approximately every
20 minutes, a bacterial cell reproduces by dividing
into two cells. Put two beans into Cup 2 to represent the second generation of bacteria.
3. Calculate how many bacterial cells there would be in the third generation if each
cell in Cup 2 divided into two cells. Place the correct number of beans in Cup 3.
4. Repeat Step 3 five more times for each of your cups. All the cups should now
contain beans. How many cells are in the eighth generation? How much time has
elapsed since the first generation?
Think It Over
In the box below, create a line graph to show how the population of bacteria increased in this
activity. The x-axis (horizontal) should represent time (use 20 minute generation time, not the
time it took you to do the activity). The y-axis (vertical) should represent population size.
Based on this activity, explain why the number of bacteria can increase rapidly in a
short period of time.
Materials
260 dried beans
8 plastic cups
marking pen
graph paper
11
Bacteria
1. INFER Why would being able to reproduce quickly be advantageous to an organism? Explain.
2. INFER How could an organism able to reproduce quickly be dangerous
to other organisms? Explain.
3. INFER Would it be harder for your body to fight off an infection from
an organism that reproduced quickly or one that reproduced slowly? Explain.
4. DRAWING CONCLUSIONS Based on the results you observed in the
lab, do you think there are more people or more bacteria on Earth? Explain.
Inquiry Warm-Up, How Quickly Can Bacteria Multiply?
In the Inquiry Warm-Up, you investigated how quickly bacteria can reproduce. Using what you learned from that activity, answer the questions below.
12
Classifying Bacteria Bacteria are everywhere. Despite their microscopic size, bacteria vary
greatly in size, color, and shape. In this activity, you will observe a few
types of bacteria using a light microscope.
INQUIRY FOCUS Observe
Procedure
1. Your teacher will provide you with at least three different slides of bacteria.
2. Look at each slide under the light microscope. Make a sketch of the bacteria you see on each
slide. If possible, give a size estimate for each
type of bacterium.
Think It Over
Compare and contrast the different bacteria you observed.
Look at the photos provided by your teacher that show the different shapes of
bacteria. Label your sketches of the bacteria as cocci, bacilli, or spirilla.
Materials
light microscope
prepared slides of different bacteria species
Photographs of cocci, bacilli, and spirilla
13
Role of Bacteria in Nature NOTES
Process Role of Bacteria
(explain how, if it is helpful/harmful, etc.)
Oxygen Production
Food Production
Health & Medicine
Environmental Cleanup
Environmental Recycling
14
Drawing Conclusions: Bacteria
Decomposers are organisms that break down large molecules within
organisms into smaller molecules. In this activity, you will investigate the
role of bacteria as decomposers.
INQUIRY FOCUS Draw Conclusions
Procedure
1. Put several lettuce leaves on two different plates.
2. Place one plate in the refrigerator. Place the other plate in a warm place. Allow the plates to sit for several days.
3. After several days, observe the lettuce. Describe what you see.
_________________________________________________________________________
_________________________________________________________________________
4. Wash your hands with warm water and soap.
Think It Over What has made the lettuce change as it did?
________________________________________________________________________
________________________________________________________________________
How does temperature affect the growth of some bacteria?
________________________________________________________________________
________________________________________________________________________
What can you conclude about how bacteria on the lettuce are similar to the
bacteria that act as decomposers in the environment?
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
________________________________________________________________________
Materials
lettuce leaves
2 plates
15
Bacteria
1. How are bacterial cells different from the cells of eukaryotes?
2. List four ways that bacteria are helpful to people.
3. bacteria
4. cytoplasm
5. ribosomes
6. flagellum
7. cellular respiration
8. binary fission
9. conjugation
10. endospore
11. pasteurization
12. decomposers
a. tiny structures that produce proteins inside bacteria
b. a process by which bacteria reproduce asexually
c. organisms that break down large, complex chemicals in dead organisms into small, simple chemicals
d. the region inside the cell membrane of a bacterium
e. a process by which bacteria reproduce sexually
f. the process of breaking down food to release energy
g. tiny single-celled organisms that live almost everywhere
h. a method of slowing down food spoilage
i. a small, rounded, thick-walled resting cell inside a bacterial cell
j. a whip-like structure that helps a bacterial cell to move
Building Vocabulary
Match each term with its definition by writing the letter of the correct definition in the right column on the line beside the term in the left column.
Understanding Main Ideas
Answer the following questions.
16
Bacteria
Identifying Bacteria
Thousands of different kinds of bacteria inhabit Earth. Each kind can be distinguished from the others by
its characteristics. In addition to shape, these characteristics include: whether it will grow in water hotter
than 45°C; whether it will grow in very salty water; whether it will grow in the presence of air; whether
it will grow without air; and whether it forms endospores.
Scientists who study bacteria use these and about 15 other characteristics to identify a bacterium. In
the table below, a plus (+) sign means the bacterium has the characteristic. A minus (−) sign means the
bacterium does not have the characteristic.
1. What characteristic do all of the bacteria have in common?
2. How could you distinguish bacterium 1 from bacterium 2?
3. Which bacteria might be found in hot springs?
4. What characteristic(s) can you use to distinguish the spherical bacteria from one another?
5. Sea water is about 3.5% salt. In some places, sea water gets trapped when the tide goes out. The heat of the sun will cause some of this water to evaporate. Which bacteria are most likely to survive in such water? Explain your answer.
The table below shows the characteristics of some bacteria. Read the passage below and study the table. Then, answer the questions that follow.
Bacterium Rod Sphere Grows at 45°C
Grows in 6.5% Salt Water
Grows in Air
Grows Without Air
Endospores
1 + − + unknown + + +
2 + − + unknown − + +
3 − + − + + + −
4 + – + − − + −
5 − + − − + + −
6 − + + − + + −
17
Name Date Class
Lab Investigation
Comparing Disinfectants Reviewing Content
Television commercials and magazine ads make
claims that disinfectants are effective in killing
almost all bacteria and viruses. Yet, when you
use a disinfectant on your kitchen counter, how
can you be sure it has done its job? Because
bacteria are invisible to the naked eye, it is
impossible to know immediately whether or not
a disinfectant is actually killing the germs.
It is possible, however, to conduct an
experiment to measure the effectiveness
of a disinfectant. Agar plates are petri dishes
filled with agar. You can inoculate an agar plate
with bacteria. Over a period of a few days, the
bacteria will reproduce so much that you will
actually be able to see colonies of bacteria with
your naked eye. In this activity, you will put disinfectants
on some of the agar plates to determine how effective the
disinfectants are at preventing bacterial growth.
Reviewing Inquiry Focus
Drawing conclusions involves explaining what the data from an
experiment mean. Why are the data important? What trends do the
data show? In the process of drawing a conclusion, scientists analyze
and interpret the data from their experiment and determine whether
their hypothesis is supported. If the hypothesis is unsupported
by the data, a scientist might decide whether to do more research
on the subject. Read through the lab procedure and answer the
following questions.
If a bacterium can divide every
10 minutes, how many bacteria
could there be in a petri dish after two hours if there was only one
bacterium to begin with? How
many will there be in four hours?
What will you be drawing a conclusion about in this lab?
A scientist conducts an experiment to determine the effect of fertilizer on tomato
plant growth. Which of the following could be a conclusion from that experiment?
• Fertilizer improves tomato plant growth.
• Tomato plant A grew 10 cm.
• Fertilizer provides plants with additional nitrogen.
18
Comparing Disinfectants
Problem
How well do different disinfectants limit the growth of bacteria?
How well do different of ba?
1. Work with a partner. Obtain three petri dishes containing sterile nutrient agar. Without opening them, use a grease pencil to label the bottoms A, B, and C. Write your initials on each plate.
2. Wash your hands thoroughly with soap, and then run a fingertip across the surface of your worktable. Your partner should hold open the cover of Petri Dish A, while you run that fingertip gently across the agar in a zig-zag motion. Close the dish immediately.
3. Repeat Step 2 for dishes B and C.
4. Use a plastic dropper to transfer two drops of one disinfectant to the center of Petri Dish A. Open the cover just long enough to add the disinfectant to the dish. Close the cover immediately. Record the name of the disinfectant in your data table. CAUTION: Do not inhale vapors from the disinfectant.
5. Repeat Step 4 for Dish B but add two drops of the other disinfectant. CAUTION: Do not mix any disinfectants together.
6. Do not add any disinfectant to Dish C.
7. Seal the covers by taping them all around so that they will remain tightly closed. Allow the three dishes to sit upright on your work surface for at least five minutes. CAUTION: Do not open the petri dishes again. Wash your hands with soap and water.
INQUIRY FOCUS Observe, Control Variables, Draw Conclusions
Materials
clock
grease pencil
2 plastic droppers
transparent tape
2 household disinfectants
3 plastic petri dishes with sterile nutrient agar
Procedure
19
Name Date Class
Lab Investigation
8. As directed by your teacher, store the petri dishes upside down in a warm, dark place where they can remain for at least three days. Remove them only to make a brief examination each day.
9. After one day, observe the contents of each dish without removing the covers. Estimate the percentage of the agar surface that shows any changes. Record your observations. Return the dishes to their storage place when you have finished making your observations. Wash your hands with warm water and soap.
10. Repeat Step 9 after the second day and again after the third day.
11. After you and your partner have made your last observations, return the petri dishes to your teacher unopened. Wash your hands with soap and water.
COMPARING DISINFECTANTS continued
Data Table
Petri Dish Disinfectant Day 1 Day 2 Day 3
A
B
C
20
Name Date Class
Lab Investigation
Analyze and Conclude
Observe How did the appearance of Dish C change during the lab?
Interpret Data How did the appearance of Dishes A and B compare
with Dish C?
Draw Conclusions How did the appearance of Dishes A and B
compare with each other? What can you conclude about the two
disinfectants from your observations?
Control Variables Why was it important to use one petri dish that did
not contain any disinfectant?
COMPARING
DISINFECTANTS continued
21
Name Date Class
Lab Investigation
Comparing Disinfectants
Design an Experiment Describe how you would design an experiment
to test how well antibacterial soaps control the growth of bacteria.
Interpret Data Compare your results with those of the other groups
in your class. How can you account for similarities and differences
among the results?
Summarize Explain what you have learned about the use of disinfectants
to control bacteria and what you would still like to know.
What I learned
What I still want to know
22
Virus and Bacteria Venn Diagram
Living or non-living?
_____________________________
Living or non-living?
________________________________
What are the basic shapes?
________________________________
_______________________________
How does it reproduce?
________________________________
________________________________
How does it reproduce?
________________________________
________________________________
How does it act in your body?
________________________________
________________________________
How does it act in your body?
________________________________
________________________________ How is it treated/prevented?
________________________________
_______________________________
How is it treated/prevented?
________________________________
_________________________________
What are some common examples?
______________________________
_______________________
What are some common examples?
_______________________________
_______________________
What are the basic shapes?
________________________________
________________________________
Virus Bacteria
Size?
_____
_____
Enters body?
__________
__________ Prevented by?
___________
__________
___________ Called?
________
_______