VvIBD in Peru AAAP Marco Cisneros

8/2/2019 VvIBD in Peru AAAP Marco Cisneros

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RIPROSAT ECUADOR

First detection of the very virulent

form of Infectious Bursal Disease

Virus (vvIBDV) in Peru

Marco Cisneros T. DMVZ MSc.


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IBDV is widely present in Peru, classical and variant(both A and E) strains are well recognized.

(Icochea 2001, Banda 2006, Ceva findings until 2006)

Since 2004 clinical cases of Bursal Disease werereported in Puerto Maldonado region (south east and faraway from poultry producer areas). In August 2007 theregion was visited and bursa samples were collected

High mortalities between 28 35 d.o. Layer pullets up to 60% Broilers up to 30%

Samples were taken from three different farms 19 d.o. layers pullets apparently normal 35 39 d.o. broilers with history of clinical IBD at 28 days 27 31 d.o. broilers showing clinical signs of the disease.

INTRODUCTION


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PuertoMaldonado

BRAZILPERU

BOLIVIA

COLOMBIA

VENEZUELA


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Commercial Pullets (19 d.o.)

No clinical signs or lesions indicative of IBDV infection


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Broilers (35 39 d)

Whitish diarrhea (the only clinical sign) Muscular hemorrhages (most frequent lesion)

Some lesions in kidneys and proventriculus No macroscopic lesions in bursas (the bursa shown inthe picture is not normal, it is edematous!


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Broilers (2731 d)

Depressed birds, ruffled feathers, prostration, fallen wings, whitish diarrhea

Mortality rising dramatically


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Swollen and edematous bursas

Extensive hemorrhagic areas in bursas and alterations in folds


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Muscular hemorrhages (more frequently observed lesion)

Proventriculitis, spleen lesions


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DIAGNOSIS

Bursa samples tested at three different labs formolecular detection and characterisation of IBDV.

CEVA Phylaxia : Budapest Hungary (frozen bursas) RT PCR - Sequencing

SIMBIOS Biotechnology : Canoas, RS Brazil (FTA)

RT PCR - RFLP Sao Paulo University : Sao Paulo Brazil (FTA)

RT PCR - Sequencing

UNIVERSIDADE DE SO PAULOFaculdade de Medicina Veterinria eZootecniaDepartamento de PatologiaLaboratrio de OrnitopatologiaPHYLAXIA

R&D Virology


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RESULTS

Molecular analysis: VP2 hypervariable region Ceva-Phylaxia: 408 bp (721 1128) Simbios: 248 bp (804 1052) USP: vvIBD200 bp, Classic500 bp

FARMS LABORATORY RESULT Interpretation

Layer Pullets

Phylaxia

USP

Simbios

---

Negative

---

Broilers 35 39 d.o

Phylaxia

USP

Simbios

vvIBD

vvIBD

Group 11 vvIBD

Broilers 27 31 d.oPhylaxiaUSP

Simbios

vvIBDvvIBD + Classic

Groups 11 + 5 vvIBD + Lukert?


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Phylogenetic Tree - Phylaxia

Distance 0.02

00273/73AU

BURSINE2

228E

849VB/87BE

G92/2/03BR

GLS/87US

GUMBCT/gen

BR-7

TAD/DE

3212/88US

BR-2

D78

52/70GB

OKYM/91JP

VarA/85US

VEN7

CS89/89GB

BR-3

DV86/86NLOKYMT/95JP

VEN6VEN9

TULA94/RU

UK661/86GBD819/3/07PED819/2/07PE

G138/3/03BRG138/4/03BR

CEVACIBDLSTC/67US

U28/88USVarE/85US

P2/73DECEVACGumbo

Cu1/76DEPBG98/76GB

Very virulentIBDVs


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% Relationship vvIBDVs - Phylaxia

Seq->OKYMT/95 JP

UK661/86 GB

849VB/87 BE

Cu1/76 DE VEN9

G92/2/03 BR

G138/4/03 B

G138/3/03 B

D819I2PE

D819I3PE BR-7

OKYMT/95JP ID 0.977 0.95 0.924 0.985 0.977 0.963 0.963 0.965 0.965 0.975

UK661/86GB 0.977 ID 0.958 0.926 0.987 0.99 0.975 0.975 0.977 0.977 0.987

849VB/87BE 0.95 0.958 ID 0.924 0.96 0.963 0.948 0.948 0.95 0.95 0.96

Cu1/76DE 0.924 0.926 0.924 ID 0.924 0.931 0.916 0.916 0.928 0.928 0.928

VEN9 0.985 0.987 0.96 0.924 ID 0.987 0.973 0.973 0.975 0.975 0.985

G92/2/03BR 0.977 0.99 0.963 0.931 0.987 ID 0.985 0.985 0.987 0.987 0.997G138/4/03B 0.963 0.975 0.948 0.916 0.973 0.985 ID 0.995 0.973 0.973 0.987

G138/3/03B 0.963 0.975 0.948 0.916 0.973 0.985 0.995 ID 0.973 0.973 0.987

D819I2PE 0.965 0.977 0.95 0.928 0.975 0.987 0.973 0.973 ID 1 0.985

D819I3PE 0.965 0.977 0.95 0.928 0.975 0.987 0.973 0.973 1 ID 0.985

BR-7 0.975 0.987 0.96 0.928 0.985 0.997 0.987 0.987 0.985 0.985 ID


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ASSIS

IAPARI

RIO ACRE


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Current Situation of vvIBD in Latin America

BRAZIL (1997)

Di Fabio et al, Vet.Rec. 1999, 145(7):203-204

DOMINICAN REPUBLIC (1998)

P.Mant et al, Med.Vet. 2000, 17(1):20-25

VENEZUELA (2001)

Y.Gardin et al. World Poultry. 2003, 19(3):36-38

COLOMBIA (2004)

Ochoa et al. Rev. Plumazos. 2005, 23:10-14

URUGUAY (2004)

M.Hernandez et al, Av.Dis. 2006, 50:624-631

BOLIVIA (2004)

ARGENTINA (2006) Molecular identification (RFLP)

P.Remorini et al, Av.Dis. 2006, 50:245251

PERU (2007 2004?)

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VvIBD in Peru AAAP Marco Cisneros