When labeling a petri plate you should write on the:

Side

Bottom Top

Insid

e of the ...

0% 0%

33%

67%1. Side2. Bottom3. Top4. Inside of the bottom

Plasmids are called ____ when they carry a desired gene into recipient cells.

Parasit

es

Vectors

Cultured

Bacte

ria

0%7%

14%

79%1. Parasites2. Vectors3. Cultured4. Bacteria

Bacteria are often classified according to cell shape—round, rod, and spiral. Which of the following is rod

shaped?

Diploco

ccus P

neumoniae

Staphylo

cocc

us Aure

us

Stre

ptoco

ccus H

emolyticus

Bacillu

s Subtilis

0%

100%

0%0%

1. Diplococcus Pneumoniae

2. Staphylococcus Aureus

3. Streptococcus Hemolyticus

4. Bacillus Subtilis

Each of the following is a direct positive outcome of the genetic modification of crop

plants EXCEPT:

Herb

icide Resi.

..

Pest Resis

tanc...

Enhan

ced ch

lor...

Vaccine En

rich...

29%

36%36%

0%

1. Herbicide Resistance

2. Pest Resistance3. Enhanced

chlorophyll production

4. Vaccine Enrichment

What component used in protein gel electrophoresis causes the proteins to run

toward the positive electrode?

Polyacry

lamide

The Tris in

the buffer

The glycin

e in th

e buffer

The SDS

29%

36%

7%

29%

1. Polyacrylamide2. The Tris in the

buffer3. The glycine in the

buffer4. The SDS

A particular processed mRNA molecule is 704 nucleotides long. How many amino acids are most likely to be in the protein

resulting from the translation of this mRNA molecule?

2112230

703704

7%0%

7%

86%1. 21122. 2303. 7034. 704

Eukaryotic cells and prokaryotic cells are different in that eukaryotic cells:

Do not h

ave or..

.

Are sm

aller t

h...

Have

a nuclear..

.

Do not h

ave D

N...

0% 0%

100%

0%

1. Do not have organelles

2. Are smaller than prokaryotic cells

3. Have a nuclear membrane

4. Do not have DNA

In DNA gel electrophoresis the ____ fragments migrate the ____ distances and the ____

fragments migrate the ____ distances.

Shorte

st/gre

at...

Longe

st/greate

...

Nonpolar

/great..

.

Polar/greate

st...

64%

7%7%

21%

1. Shortest/greatest/longest/least

2. Longest/greatest/shortest/least

3. Nonpolar/greatest/polar/least

4. Polar/greatest/Nonpolar/least

Which piece of equipment would be used to separate bacteria from the media they are in?

Autocla

ve

Thermal C

ycler

Centrifu

ge

Electr

ophoresi.

..

8%

23%23%

46%1. Autoclave2. Thermal Cycler3. Centrifuge4. Electrophoresis

The solution used to sterilize the bench top when you have completed your experiment is:

10% bleach so

l...

Hyd

roch

loric A

... So

ap

50% bleach

86%

14%

0%0%

1. 10% bleach solution2. Hydrochloric Acid3. Soap4. 50% bleach

The biotechnology product that came from the hot springs of Yellowstone National Park is:

DNA lig

ase

RNA polymera

se

Thermus A

quaticus D

NA...

Bacillu

s Sulfo

lobus kinase

7%

36%

50%

7%

1. DNA ligase2. RNA polymerase3. Thermus Aquaticus

DNA polymerase4. Bacillus Sulfolobus

kinase

The mole establishes a relationship between the:

Atomic

mass un...

The size

of an...

The molecu

lar ...

The charge

of ...

64%

0%0%

36%

1. Atomic mass unit and the gram

2. The size of an atom and its mass

3. The molecular mass of a compound and empirical formula

4. The charge of an atom and its ionization energy

The building blocks of proteins are:

Nucle

otides

Amino acids

Deoxyri

bose m

olecules

suga

rs

0% 0%7%

93%1. Nucleotides2. Amino acids3. Deoxyribose

molecules4. sugars

What type of bond is found between water molecules?

Ionic

Covalent

Hyd

rogen

Van der Waa

ls

21%

0%

57%

21%

1. Ionic2. Covalent3. Hydrogen4. Van der Waals

What enzyme is responsible for carrying DNA replication?

RNA polymera

se

DNA polym

erase

DNA lig

ase

RNA ligase

14%7%

0%

79%1. RNA polymerase2. DNA polymerase3. DNA ligase4. RNA ligase

In preparing agar plate growth media, the best time to add antibiotics is:

Before auto

cla...

Immediate

ly fo

...

Immediate

ly be...

After plates h

...

7% 7%

57%

29%

1. Before autoclaving so as to sterilize antibiotics

2. Immediately following autoclaving while medium is still hot

3. Immediately before pouring while media is cooled but still liquid

4. After plates have been poured and cooled

DNA travels from the ____ to the _____ terminal during gel electrophoresis.

Positive

/nega

t...

Neutra

l/negati...

Neutra

l/positi

...

Negative/p

osit...

21%

71%

7%0%

1. Positive/negative2. Neutral/negative3. Neutral/positive4. Negative/positive

The structure that acts as the cell’s security guard regulating what enters and leaves the cell

is known as the:

Nucle

us

Golgi b

odies

Cell membra

ne

Prote

in barrie...

7%0%

93%

0%

1. Nucleus2. Golgi bodies3. Cell membrane4. Protein barrier

PCR primers are short lengths of single-stranded DNA that are specifically designed to compliment, and anneal to, short base sequences that flank the PCR’s “target” DNA. Identify the

best primer to use in a PCR reaction that includes the following DNA sample: 3’ GAGTACCGATTAACACGGTAtargetregionCAGGATACGAGCTCTACCG 5’

5’ ATGGCTAATTG...

3’ ATGGCTAATTG...

3’ TATGCTCGAGA...

5’ TAAT 3’

79%

7%7%7%

1. 5’ ATGGCTAATTGTGC 3’2. 3’ ATGGCTAATTGTGC 5’3. 3’ TATGCTCGAGAT 5’4. 5’ TAAT 3’

There are 3 types of RNA and each type is involved in the synthesis of proteins. The first type is part of the ribosome, the second type is the template for translation, and

the third type brings amino acids to the ribosome. Which answer below lists these three types of RNA in the same order as described in the question?

mRNA, tR

NA, rRNA

rRNA, tR

NA, mRNA

tRNA, m

RNA, rRNA

rRNA, m

RNA, tRNA

14%

36%

7%

43%1. mRNA, tRNA, rRNA2. rRNA, tRNA, mRNA3. tRNA, mRNA, rRNA4. rRNA, mRNA, tRNA

In the primary structure of protein, the amino acids are linked together by what type of bond?

Disu

lfide bond

Hyd

rogen bond

Peptide bond

Ionic

bond

7% 7%

71%

14%

1. Disulfide bond2. Hydrogen bond3. Peptide bond4. Ionic bond

The atomic number of an atom is defined as its:

Mass

in AM

U

Number o

f elec..

.

Number o

f neut..

.

Number o

f pro

t...

0%

71%

14%14%

1. Mass in AMU2. Number of electrons3. Number of neutrons4. Number of protons

The sum of the atomic masses of all the atoms in a compound is called the:

Molecu

lar weig.

..

Empiri

cal fo

rm...

Molar v

olume

Percenta

ge co

m...

100%

0%0%0%

1. Molecular weight2. Empirical formula3. Molar volume4. Percentage

composition

Each of the following is a type of protein EXCEPT:

Stru

ctural

Enzy

me

Stero

id

Transport

0%7%

79%

14%

1. Structural2. Enzyme3. Steroid4. Transport

The cellular organelle that is of great value to geneticists because it carries only maternal DNA

is the:

Nucle

us

Golgi a

pparatu

...

Ribosome

Mito

chondrio

n

7%

79%

14%

0%

1. Nucleus2. Golgi apparatus3. Ribosome4. Mitochondrion

Your boss in the state crime lab gives you a sample of DNA obtained from the back side of a postage stamp. She asks you to do DNA fingerprinting on the sample in order to determine if it matches the DNA of a particular suspect. You recognize that you will

have to amplify the DNA sample since there is not enough to do the fingerprint analysis. What piece of equipment when you use to amplify the DNA

Centrifu

ge

Autocla

ve

Thermal C

ycler

Spectr

ophotome...

7%

29%

57%

7%

1. Centrifuge2. Autoclave3. Thermal Cycler4. Spectrophotometer

What is the name of the specific sequence of DNA that is cut by a restriction enzyme?

Palindro

me sequence

Start

codon se

quence

Recogniti

on sequence

TATA box sequence

0%7%

36%

57%1. Palindrome sequence

2. Start codon sequence

3. Recognition sequence

4. TATA box sequence

The study of the functions of specific proteins is known as:

Prote

omics

Genomics

Functi

onal syste

matics

Prote

inology

25% 25%25%25%1. Proteomics2. Genomics3. Functional

systematics4. Proteinology

You are preparing a buffer solution for your DNA experiment. The directions tell you to adjust the pH to 8.2. When you check the current pH, you observe that it is7.6. Which of the following solutions is the best choice for adding to

your buffer to adjust the pH to 8.2?

10% SDS

1M N

aOH

1M HCl

5M HCl

25% 25%25%25%1. 10% SDS2. 1M NaOH3. 1M HCl4. 5M HCl

Molecules in which the atoms share electrons equally would be called:

Acids

Bases

Hyd

rophobic

Hyd

rophilic

Polar

Non-P

olar

17% 17% 17%17%17%17%1. Acids2. Bases3. Hydrophobic4. Hydrophilic5. Polar6. Non-Polar

The best medium type for rapid, mass culturing of bacteria is:

Agar plat

e Li

quid St

ab Sl

ab

25% 25%25%25%1. Agar plate2. Liquid3. Stab4. Slab

What types of clothing are NOT appropriate in the lab?

Lab co

ats, gog..

.

Shorts

, sandal...

Jeans,

T-Shirt...

Blouse, S

kirt

25% 25%25%25%1. Lab coats, goggles2. Shorts, sandals3. Jeans, T-Shirts4. Blouse, Skirt

In preparing petri plates for a transformation experiment, you first measure out the correct amount of powered agar for the volume of water you have. Next, you will swirl the agar so that

most of it goes into solution (dissolves). Identify the equipment that you will use next.

Centrifu

ge

Thermal c

ycler

Spectr

ophotome...

Micr

owave

25% 25%25%25%1. Centrifuge2. Thermal cycler3. Spectrophotometer4. Microwave

One reason that prokaryotes have been such valuable model organisms in genetic studies is that their generation time is so short. In fact, some species can reproduce as often as every 20

minutes. This gives the researcher answers literally over night. With this in mind, which of the following eukaryotes would be the best model organism?

Human

Bread mold

Round worm

Yeast

25% 25%25%25%1. Human2. Bread mold3. Round worm4. Yeast

In order to make 3 liters of a .5M sucrose solution, how many grams of sucrose would you add. (The molecular weight of

sucrose is 342g)

1026g 513g

542g 171g

25% 25%25%25%1. 1026g2. 513g3. 542g4. 171g

DNA splits or unzips between the nitrogen bases of the DNA molecule. So as to expose the sense strand bases of the gene. This “unzipping” occurs because the bond between the bases is relatively weak and is

known as a _______ bond.

Ionic

Covalent

Hyd

rogen

Peptide

25% 25%25%25%1. Ionic2. Covalent3. Hydrogen4. Peptide

The basic building block of DNA is a(n):

Nucle

otide

Amino acid ATP

Deoxyri

bose

25% 25%25%25%1. Nucleotide2. Amino acid3. ATP4. Deoxyribose

When amplifying a sample of human DNA by PCR at a particular marker site, it is NOT absolutely critical that extraneous DNA, say from bacteria for sample, not contaminate the sample to be amplified. This is because:

The primers

used in

the...

The DNA polym

erase m

o...

Any contam

inant DNA ...

It is

statisti

cally

impro

ba...

25% 25%25%25%1. The primers used in the PCR reaction are unique to human DNA

2. The DNA polymerase molecule used in PCR can only copy human DNA sequences

3. Any contaminant DNA would immediately be degraded by enzymes present in the reaction mixture

4. It is statistically improbable that there would be enough contaminant DNA to serve as template DNA

Before turning the centrifuge machine on, you should always check to make sure:

Is it

plugged ...

The lid is

clo...

That the tu

bes...

That you have ...

25% 25%25%25%1. Is it plugged in2. The lid is closed3. That the tubes are

placed in balanced positions

4. That you have set the correct time

When should safety glasses be worn?

Durin

g any exp...

Any time you a...

Only w

hen work

...

Only w

hen work

...

25% 25%25%25%1. During any experiment with harmful chemicals.

2. Any time you are working in the lab on an experiment.

3. Only when working with bacteria, or glass.

4. Only when working with reagents, bacteria, or glass.

Gram negative bacteria stain ___ because of the ___ cell wall?

Red/thin

Purple/th

in

Red/thick

Purple/th

ick

25% 25%25%25%1. Red/thin2. Purple/thin3. Red/thick4. Purple/thick

How many moles of NaOH is in 120g of NaOH?

2 3 5120

25% 25%25%25%1. 22. 33. 54. 120

Proteins are synthesized (produced) in the

Ribosomes

Endoplasm

ic Reticu

lum

Lyso

somes

centri

oles

25% 25%25%25%1. Ribosomes2. Endoplasmic

Reticulum3. Lysosomes4. centrioles

The purpose of the antibiotic in a bacterial transformation experiment is to:

Kill any fo

rei...

Promote

the ba...

Preve

nt all b

u...

Promote

the gr..

.

25% 25%25%25%1. Kill any foreign microbes

2. Promote the bacteria to produce antibodies

3. Prevent all but the transformants from growing

4. Promote the growth of all but the transformants

Compounds that release hydrogen ions into solutions are called

Acids

Bases

Hyd

rophobic

Hyd

rophilic

25% 25%25%25%1. Acids2. Bases3. Hydrophobic4. Hydrophilic

The “magical” characteristic of Taq Polymerase is that it

Can reco

gnize ...

Is highly re

si...

Is highly re

si...

Maintai

ns its

...

25% 25%25%25%1. Can recognize cut sites from both directions

2. Is highly resistant to antibiotics such as ampicilin

3. Is highly resistant to mutagens such as radiation

4. Maintains its tertiary structure and ability to function under boiling conditions

5 mg is equivalent to

.005 ug

5 ug

500 ug

5,000 ug

25% 25%25%25%1. .005 ug2. 5 ug3. 500 ug4. 5,000 ug

The best way to obtain an accurate determination regarding the concentration of bacteria within a flask of liquid medium is to

Swirl

the m

edi...

Compare th

e de...

Run the bacte

r...

Obtai

n transm

i...

25% 25%25%25%1. Swirl the medium and make an approximation

2. Compare the density of the cultured medium with that of the uncultured medium

3. Run the bacterial DNA out in agarose gel electrophoresis

4. Obtain transmittance readings on the spectrophotometer

To streak bacteria on an agar plate, you would use a(n)

Micr

opipetter

Inocu

lating lo...

Pipette

Micr

opipette t...

25% 25%25%25%1. Micropipetter2. Inoculating loop3. Pipette4. Micropipette tip

Small circular loops of DNA (typically 3000 to 15,000 bp in length) naturally found in

bacteria are known as

Chromoso

mes

Genes

Plasmids

Chromatin

25% 25%25%25%1. Chromosomes2. Genes3. Plasmids4. Chromatin

The best way to measure out 0.157mL of a particular solution would be to use the ___ micropipetter with the dial set on ___ uL.

0.5 to 10/1

.57

2 to 20/1

5.7

20 to 200/1

57

100 to 1000/1

5...

25% 25%25%25%1. 0.5 to 10/1.572. 2 to 20/15.73. 20 to 200/1574. 100 to 1000/1570

In order to prevent contamination, it is absolutely essential to change pipet

tips, particularly

When m

ixing t

h...

Between aloquo...

Between aloquo...

When adding an...

25% 25%25%25%1. When mixing the ingredients within a sample

2. Between aloquots of the first ingredient added to the tubes of several samples

3. Between aloquots of the last ingredient added to the tubes of several samples

4. When adding antibiotics to growth media

Which term describes a molecule that is water-hating?

Hyd

rophobic

Polar

Hyd

rophilic

Ionic

25% 25%25%25%1. Hydrophobic2. Polar3. Hydrophilic4. Ionic

Which choice below lists the correct order of the steps performed during a

bacterial transformation?

Preincu

bation,...

Preincu

bation,...

Preincu

bation,...

Preincu

bation,...

25% 25%25%25%1. Preincubation, heat shock, incubation, recovery

2. Preincubation, incubation, heat shock, recovery

3. Preincubation, recovery, incubation, heat shock

4. Preincubation, heat shock, recovery, incubation

Microarray technology has recently become a tremendously valuable tool in the hands of

scientists because it reveals the

Loca

tion of ge...

Degre

e of “ide...

Degre

e to w

hic...

Base se

quence ...

25% 25%25%25%1. Location of genes upon chromosomes

2. Degree of “identicalness” between two samples of DNA of different sources

3. Degree to which several genes are individually expressed within a particular cell or tissue

4. Base sequence differences between diseased and normal genes

Regions of DNA that are transcribed into mRNA and then translated into

proteins are called?

Anticodons

Primers

Genes

Promote

rs

25% 25%25%25%1. Anticodons2. Primers3. Genes4. Promoters

Identify the statement that is FALSE with respect to the difference

between DNA and RNA.

DNA is

general...

DNA has t

hymin...

DNA has h

ydro

g...

DNA dire

ctly c.

..

25% 25%25%25%1. DNA is generally a double stranded, whereas RNA is generally single stranded.

2. DNA has thymine, whereas RNA has uracil.

3. DNA has hydrogen bonds, whereas RNA generally does not.

4. DNA directly codes for proteins, whereas RNA most often directly codes for DNA.

When drawing up a solution with a micropipetter, you should

Push th

e plung...

Put the tip in

...

Push th

e plung...

Put the tip in

...

25% 25%25%25%1. Push the plunger to the first stop before placing the tip in the solution and release the plunger slowly.

2. Put the tip in the solution first, then push the plunger to the first stop and release quickly.

3. Push the plunger to the second stop, put the tip in the solution then release the plunger quickly.

4. Put the tip in the solution first, then push the plunger to the second stop and release slowly.

The primary role of agar in plate growth media is to

Give

the m

edia a noncle..

Provide nutri

ents

Keep the m

edia from dr..

.

Give

the m

edia a gel con...

25% 25%25%25%1. Give the media a nonclear golden color

2. Provide nutrients3. Keep the media

from drying out4. Give the media a gel

consistency

What is the proper laboratory procedure when disposing of

microorganisms?

Place in

a clo...

Wra

p in a tr

as...

Expose

micr

oor...

Leave on th

e b...

25% 25%25%25%1. Place in a closed trash can.

2. Wrap in a trash bag and place in trash can.

3. Expose microorganisms to bleach or autoclave.

4. Leave on the bench top until they die.

In a serial dilution, the concentration of each successive sample is

Decre

ased by a...

Incre

ased by a...

Made to

remain...

Made to

remain...

25% 25%25%25%1. Decreased by a given fraction

2. Increased by a given fraction

3. Made to remain constant

4. Made to remain constant, then to become more dilute over time

Which method for transferring new DNA into bacteria involves the bacteria taking up pieces of DNA that are

floating freely in solution outside of the cell?

Conjugation

Transducti

on

Transform

ation...

33% 33%33%1. Conjugation2. Transduction3. Transformation

To date, the most valuable and widespread application of green fleurescent protein

(GFP) is

As a novelty

; ...

As a ta

g or ma...

As a tu

mor cel...

As an antigen ...

25% 25%25%25%1. As a novelty; green fleurescent things are really cool

2. As a tag or marker to indicate the presence of an accompanying introduced gene of interest

3. As a tumor cell probe revealing the presence of certain cancers

4. As an antigen to antibiotics such as ampicilin

The bond between the complimentary bases of a DNA molecule is

Ionic

Phosphoric

Covalent

Hyd

rogen

25% 25%25%25%1. Ionic2. Phosphoric3. Covalent4. Hydrogen