Cosmetic Laser Solutions MedSpa 857-288-8257www.cosmeticlasersolutions.net
Collagen Booster Serum
Collagen Booster
®
Before After
Before
Before
After
After
Production of total collagen, collagens I and III (ex vivo tests)
The action of NF-13 Complex on total collagen production was evaluated on skin explants from a 74 years old donor. Skin explants were either
day 6 and day 11. Total collagen was detected with staining assessed by microscopical observation and image analysis.
Results: NF-13 Complex dramatically increases the vitality
of young and aged fibroblasts. Aged fibroblasts
are enabled to function property again.
Results: NF-13 Complex increases the synthesis of
Pro-collagen I in a dose dependent way.
Aged fibroblasts show even better ratio of
production compared to young fibroblasts.
Results: NF-13 Complex used at 0.5% increases the
quantity of total collagen by 6.8% in 11 days.
*p<0.01 compared to untreated, Student t-test
**p<0.001 compared to untreated, Student t-test
Biological activity
17 20
36
80
60
40
20
0
Improvement of fibroblasts vitality
Improvement of mean viability(% improvement
versus untreated cells)
0.08% 0.4% 2%
Young fibroblasts
Aged fibroblasts
50
40
30
20
10
0
*
**
Production of Pro-collagen I in vitro
Concentration of Pro-collagen I
in ng/ml(% improvement
versus untreated cells)
0.1% 0.5% % of NF-13 Complex2%
No treatment
NF-13 Complex0.5%
90
85
80
75
Total collagen
Percentage of surface occupied by total
collagen (%)
Day 1 Day 6 Day 11
+6.8%
®
NF-13 concentration0
25
50
75
100
MM
P en
zym
atic
act
ivity
(%
con
trol
)
Inhibition of MMP Enzymatic Activity Mean reduction in mild to moderate rosacea
from baseline at 12 weeks
MMP-9 MMP-2
Capillary
Clarity
Capillary
branching
Capillary
length
0
0.25
0.50
0.75
1.00
1.25
1.50
Impr
ovem
ent f
acto
r
a
b
c
Placebo
NF-13 Complex
NF-13 inhibits more than 80% of MMP enzymes activity
(Types 2 and 9). The MMP-12 enzyme is also inhibited by
NF-13.
23 volunteers (27 enrolled) with mild to moderate rosacea,
visible telangiectases.
Randomized split-face, double-blind trial, 12 weeks with a
5% NF-13 Complex cream or placebo.
a p<0.03 - b p<0.006 - c p<0.0008
untreated or treated with 0.5% of NF-13 Complex at day 0, day 1, day 4, day 6 and day 8. Explants were then sampled and analyzed at day 0,
After studying the total collagen, two different types of collagens were quantitatively evaluated by immunostaining: collagen I (in green
fluorescent), and collagen III (in dark rose), also known as the youth collagen.
Results: NF-13 Complex at 0.5% significantly increases collagen I and collagen III synthesis by +179% and +194% respectively after 11 days,
versus untreated. Upon aging, the ratio of collagen I / collagen III is increased. With Neodermyl® at 0.5%, this ratio is decreased to the level of
a younger skin: it moves from 0.82 to 0.55.
Production of elastin (ex vivo tests)The action of NF-13 Complex on tropoelastin and elastin production was evaluated by following the same protocol as for collagen I and III.
Tropoelastin (in green fluorescent) and elastin (in green fluorescent) were detected by immunostaining assessed by microscopical observation
and image analysis.
Results: NF-13 Complex significantly increases tropoelastin and elastin production by +57% and +190% respectively after 11 days, versus untreated.
Untreated – Day 11 Untreated – Day 11NF-13 Complex 0.5% – Day 11 NF-13 Complex 0.5% – Day 11
Untreated – Day 0
NF-13 Complex 0.5%Day 11
Untreated – Day 0
NF-13 Complex 0.5%Day 11
*p<0.01 compared to untreated, Student t-test *p<0.01 compared to untreated, Student t-test
Biological activity
No treatment No treatmentNF-13 Complex NF-13 Complex
40
30
20
10
0
40
30
20
10
0
Collagen type I Collagen type III (Youth Collagen)
Percentage
of surface
occupied
by collagen
I (%)
Percentage
of surface
occupied
by collagen
III (%)
Day 0 Day 0Day 6 Day 6Day 11 Day 11
+179%*+194%*
*p<0.1 compared to untreated, Student t-test **p<0.05 compared to untreated, Student t-test
No treatment No treatmentNF-13 Complex 0.5% NF-13 Complex 0.5%
14
12
10
8
6
4
2
0
1816141210
86420
Tropoelastin Elastin
Percentage
of surface
occupied by
tropoelastin
(%)
Percentage
of surface
occupied by
elastin (%)
Day 0 Day 0Day 6 Day 6Day 11 Day 11
+57%*+190%**
®
Fast improvement of firmness and elasticity (clinical)
The two parameters R0 and R5 were measured by Cutometry to evaluate the performance of NF-13 Complex at 1% on firmness and elasticity.
Results: Using NF-13 Complex at 1% increases firmness by 13 times and elasticity by 1.6 times in only 15 days. These improvements of skin
parameters are sustained after two months of treatment.
*p<0.01 compared to placebo, Student t-test
**p<0.1 compared to placebo, Student t-test
*p<0.01 compared to T0, Student t-test
Efficacy
Placebo NF-13 Complex 1% Placebo NF-13 Complex 1%
6
4
2
0
-2
-4
-6
-8
-10
14
12
10
8
6
4
2
0
Firmness (R0) Elasticity (R5)
Improvement
of skin
firmness (R0)
(% versus T0)
Improvement
of skin
elasticity (R5)
(% versus T0)
Day 15 Day 15Day 60 Day 60
x13* x12**
+60%*
*
+44%
Wrinkles’ profiles confirm the benefits of using NF-13 Complex at 1%. After 15 days, the results are outstanding:
Results: The skin is smooth and wrinkles are visibly reduced after only 15 days of treatment with NF-13 Complex use at 1%.
NF-13 Complex delivers equivalent results to one injection of collagen filler in just 2 weeks: -15% of wrinkles reduction
(source American Society of Plastic Surgeons – 2013).
Wrinkle profile – Untreated – Day 0
Volunteer number 13 - 67 years old - crow's feet area
Wrinkle profile – NF-13 Complex 1% – Day 15
®