MAJOR MICROBIOLOGY
DEPARTMENT OF BIOLOGYPOST GRADUATE SCHOOLBOGOR AGRICULTURAL UNIVERSITY2009
MAJOR MICROBIOLOGY : AN OVERVIEW
Established in 1990 as Subprogram Study in the Study Program of
Biology
Composed on 2 division: Microbiology (Bacteriology)
Mycology
Staffs: 1 Prof, 10 doctors
Number of Student : ongoing student: 13 master, 8 doctoral
incoming student: 16 master, 3 doctoral
Programmes :
1. Master in Microbiology: microbial biodiversity and bioprospecting,
and environmental microbiology
Doctor of Philosophy in Microbiology
Supporting unit: IPB Culture Collection (currently developing through I-
MHERE project
VISION
To be recognized world-wide as the leading centre in tropical microbiological science for agriculture development and for human life quality improvement in 2020.
MISSION
To develop human resources with high capacities in tropical microbial knowledge and technologies through undergraduate and postgraduate education.
To expand and transfer microbial knowledge and technologies for continuous improvement of agriculture and human life.
Increase the quality and quantity of research in tropical microbial knowledge and technologies particularly in biodiversity and bio-prospecting.
Increase networking through research and education partnership locally, nationally and globally.
CURRICULUMMaster of Science Program (39 CSU)
General coursesEnglish 3 CSUStastical Analyses 3 CSU
Compulsory coursesAdvanced Microbiology 2 CSUMicrobial Physiology 2 CSUMicrobial Genetic 2 CSU Microbial Laboratory 2 CSUMicrobial Biotechnology 3 CSUFungal Biotechnology 3 CSUMethodology of Science 2 CSUColloqium 1 CSUSeminar 1 CSUResearch and Thesis 6 CSU
Elective courses Bio- prosepecting
Industrial Microbiology 3 CSU
Molecular Biotechnology 3 CSUBiotechnology of Antimicrobial Susbtance 3 CSU
Environmental MicrobiologyHost-Microbes Interaction 3 CSU Environmental Microbiology 3 CSUSpoilage Fungi and Mycotoxins in foodstuff 3 CSU
CURRICULUMDoctor of Philospophy Program (41 CSU)
General Course
Philosophy of Science 2 CSU
Compulsory courses
Advanced Microbial Physiology 3 CSU
Advanced Fungal Biology 3 CSU
Molecular Biology of Prokaryotic
Diversity 3 CSU
Microbial Genetic Engineering 3 CSU
Colloqium 1 CSU
Seminar 1 CSU
Research and Thesis 12 CSU
Elective courses
Biology of Pathogenic Bacteria 2 CSU
Gene Expression and Regulation 3 CSU
Biology and Productivity of
Actinomycetes 3 CSU
Biology of Mycorrhiza3 CSU
Biology and Productivity of
Macrofungi 3 CSU
Teaching Staffs and Field Interest
1. Prof. Dr. Antonius Suwanto, MSc., Ph.D (Microbiology and Molecular genetics)
2. Dr. Anja Meryandini, MS (Microbial Physiology and Enzymology)3. Dr. Aris Tri Wahyudi, MSi (Microbial Biotechnology)4. Dr. Iman Rusmana, MSi. (Environmental Microbiology)5. Dr. Nisa Rachmania, MSi. (Microbiology and Microbial Physiology)6. Dr. dr. Sri Budiarti (Medical Microbiology, Bacterial-Human Cell Interaction)7. Dr. Yulin Lestari (Microbial Ecophysiology)8. Dr. Gayuh Rahayu (Mycology and Phytopathology)9. Dr. Lisdar A Manaf (Mushroom Biology and Fungal Ecophysiology) 10. Dr. Nampiah Sukarno (Mycology and Mycorrhizal Biology)11. Dr. Okky S. Darmaputra (Post Harvest Mycology)
RESEARCH
BACTERIOLOGY
Marine Bioechnology
Producing Anti- microbial Compounds
Producing Enzyme Inhibitors
Student Involved: 4 Students
Sponge-Associated Bacteria
Targets: 1. Antimicrobial Compounds from sponge-associated Bacteria 2. Molecules/Compounds as Enzyme Inhibitors 3. Molecular Genetic of Antimicrobial Compounds Synthesis
MolecularGenetics
Aris Tri Wahyudi, PhD
Plant Growth Promoting
Rhizobacteria (PGPR)
Plant Growth Promotion Bioactive Compounds
Anti Phytopathogen
Student involved: 7 Students (2 PhD ; 2 S-2, 3 S-1)
1. Formulation of PGPR as plant growth and Biocontrol2. Molecular Genetic of Bioactive Compound Synthesis3. Bioactive Compounds/Antibiotic from PGPR
Biocontrol ofPhytopathogen
Formulation and Inoculant Prod.
-Pseudomonas sp
-Bacillus sp
Aris Tri Wahyudi, PhD
Aris Tri Wahyudi, PhD
Dr. Anja Meryandini
Research Interest: Microbial enzymes- Bacterial protease- Bacterial mannanase- Bacterial xylanase
for animal feed processing
Clostridium bifermentans producing protease enzyme
Streptomyces sp. producing xylanase enzyme
451-3234P-
16
SKK1-8
Dr. Nisa Rachmania Mubarik, MSi
Head of Microbiology Division, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Research Interest:
Symbiotic Effectiveness of Inoculant Acid-Alumunium Tolerant Bradyrhizobium japonicum Strains with Soybean Cultivar Slamet in Acid Soils (3 master students, 2007-2009)
Isolations, Characterizations and Applications Microbial Enzymes (Protease, Amylase, Chitinase) (7 undergraduate student, 1 master students, 2007-2009)
Inoculant B. japonicum on peat carrier.
0.5 mm
0.5 mm
0.5 mm
0.5 mm
0.5 mm
0.5 mm
0.5 mmChitin hydrolization at 12 hours by cell
cultures and chitinase of bacterial isolates I.5 and I.21 tested to Bemisia tabaci . Control (a), cell cultures treatment (b-d), enzyme treatment (e-g).
Dr. Iman Rusmana ([email protected]) Environmental Microbiologist
RESEARCH INTEREST:
Current Research Topics:
• Methanotrophic bacteria as biofertilizers and methane emission reducers in rice fields
• N2O reducing bacteria to reduce N2O emission from agriculture system
• Nitrate reducing bacteria and Bacillus spp. as bioactive of biofilter in close system of larval rearing and nursery of aquacultures
• Nitrate reducing and nitrifying bacteria as bioconditioner controlling nitrite and ammonia in shrimp ponds
Nitrogen cycling and N2O emission in tropical estuaries and agricultural
systemStudent involved (9 master, 1 PhD)
Amplification of mmoX gene
Amplification of pmoA gene
Size of DNA fragments
Molecular characterization of methane monooxygenase genesForward sequence of BGM9 mmoX gene:
ATAANCGCAGCGTGTTNCAGGTCCGCGAGATCGCCGACCAGTCCGCCGCCGNCAGCNAGCAGACCGCCAGCTCCAGCAACGAACTGGCGCACCTGGGCAACGAGCTGCAACTGCAGGTGGGACGTTTCCGCCTGTGACACGCCNGTCGGTCGGTACGCCCGCTCCGGCCGATAACGGCAGGACAGTTGTCATGCGCATGGCGTAGGATCGGACACTCGCCATGAAGGGAGAGTGACCATGCGCCCGCTGACCATCGATATCGACGAACTGGACCTTCGCGCTTGATACCCAGGGTATCGATCACTACCTCGACCTGTTCAGTGGCCAGGTGCTGCTGATACCGGAGGACGACCCCGACCCGGAGCTCGCCGAACTGCTGCACCGCGAGCCGGAGCGCTACGAGTG
Reverse sequence of BGM9 mmoX gene:GGTATCANCAGCACCATGGCCACTGAACAGGTCGAGGTAGTGATCGATACCCTGGGTATCAAGCGCGAAGGCCAGTTCGTCGATATCGATGGTCAGCGGGCGCATGGTCACTCTCCCTTCATGGCGAGTGTCCGATCCTACGCCATGCGCATGACAACTGTCCTGCCGTTATCGGCCGGAGCGGGCGTACCGACCGACAGGCGCGTCACAGGCGGAAACGTCCCACCTGCAGTTGCAGCTCGTTGCCCAGGCGCGCCAGTTCGGTGCTGGAGCTGGCGGTCTGCTCGCTGGCGGCGGCGGACTGGTCGGCGATCTCGCGGACCTGCACCACGCTGCGGTTGATCTCCTCGGCAACGCTGCTCTGCTCCTCGGCGGCCGANGCGATCTGCAGGTTCATGCCCTTCCACAGCGGATATAATA
Sampling for measurement and molecular analysis of nitrate reducing bacteria in sediment of tropical estuaries
Bacteriocins purification and its antimicrobial activity from Bacillus spp. isolated from shrimp ponds
FROM MOLECULAR TO ECOLOGY
Dr. dr. Sri Budiarti
Research Interest: Medicinal Microbiology
Enteropathogenic Eschericia coli:
- mode of infection
- Extracellular protease activity
- Monoclonal antibody antiprotease activity
-Diagnostic kit design
Dengue and environmental health
Screening of Antimicrobial substance from
bacteria and fungi
INDONESIAN ACTINOMYCETES AS SOURCE OF BIOACTIVE COMPOUNDS USED FOR SOLVING AGRICULTURE, ENVIRONMENT AND HEALTH PROBLEMS
1. Collection of Actinomycetes isolates from unique tropical environment, and endophyte actinomycetes
2. In vitro and in vivo assay for: antimicrobes, enzyme inhibitor (e.g. a-glucosidase, b-lactamase), degrading enzymes (lipase, cellulose and lignolitic enzymes)
3. Target organisms: agriculture & horticulture plants, plantation, poultry, and human )
4. Student involve: undergraduates (2) ,graduates ( 4 Master students), and 3 PhD students)
Dr.Ir. Yulin Lestari
MYCOLOGY
Diversity of Indonesian Filamentous Fungi (205 genera)
EntomophthoralesKickxellalesMortierellalesMucorales
Botryosphaeriales CapnodialesChaetosphaeriales ChaetothyrialesConiochaetales DiaporthalesDothideales EurotialesHelotiales HypocrealesHysteriales MicroascalesOnygenales OphiostomatalesOrbiliales PezizalesPleosporales SaccharomycetalesSordariales TrichosphaerialesXylariales Incertae sedis
AgaricalesPolyporalesTremellalesUstilaginalesIncertae sedis
Agaricales
Mucorales
EurotialesHypocreales
Incertae sedis
Polyporales
ZygomycotaBasidiomycota
Ascomycota
RESEARCH TITLE (Year 2009) : Population of Aspergillus flavus and Aflatoxin B1 Contents from Processed Peanut Products in Municipality of Bogor.
ACTIVITIES : • Survey of processed peanut product consumptions • Sampling method of processed peanut products • Determination of A. flavus population and aflatoxin B1 contents
EXPECTED OUTPUT :informations on A. flavus population and the levels of aflatoxin B1 in processed peanut products at retail levels
Dr. Okky Setyawati Darmaputra
FUNGI ON FOODSTUFF AND THEIR MYCOTOXIN
Research Interest: Biology and Application of Phosphate
Solubilizing Fungi Biology and Application Mycorrhizal Fungi
Dr. Ir. Nampiah Sukarno
Development of Aspergillus carrying GFP gene for studying the ecological and colonization process
T. caccao
Curcuma sp.
P. falcataria
a b. c.
a b. c . a b. c..
a b. c..
a b. c..
J. curcas
a b. c..
Post tin mining area
P. macrocarpa
P. macrocarpa
Agriculture area
Green housea. Control, b. A. niger,
c. AMF
Edible Ectomycorrhiza
Dr. Ir. Gayuh RahayuResearch Interest: Artificial Production of agarwood
(Jin-koh) in planta and in vitro culture:- Inoculant Production- Mechanisms of terpenoid production- Inoculation Techniques
Manipulation of Soil Fungi for Forest Tree Growth and Health
- Fungal decomposer of Shorea and Dryobalanops litter and plant growth regulator producers
(student involved: 1 master, 1 undergraduates)
Student involved ( 3 master students)
Thermophilic Fungi
compost
ZPT bioassay
Amylolytic Fungi and its fermentation capacity for ethanol production- Screening of potential mold and yeast
Dr.Ir. Lisdar A.M. Sudirman
Research Interest:-Mushroom Biology and Biotechnology- Mushroom Antimicrobial activity