│Ver.4.1Product Catalog
CIA 15-04E1
Livestock
03040506
07080911
03 Anigen, Inc. established
11 Acquired animal medicine import license
12 Acquired animal medicine manufacturing industry permission
09 Acquired ISO 9001:2000 TUV certi�cation
08 Moved to the new Young-tong factory
01 Purchased the site for the new Dongtan factory
04 Canine Distemper virus antibody diagnostic kit patent acquisition
04 Canine Parvovirus antibody diagnostic kit patent acquisition
06 Venture business certi�cation (No. 061627021-01133)
07 Technical innovation medium and small enterprises certi�cation (No.6065-1216)
07 Gyeonggi-do excellent medium and small enterprises selection
09 H5 and general pathogenic avian in�uenza virus antigen diagnostic
11 Name change of Anigen, Inc. to Animal Genetics, Inc.
12 Winner of the Animal medicine manufacturer self- regulation checking system Prize
02 World’s �rst In�uenza H3N2 type con�rmation assay development
07 Novel canine in�uenza viruses and vaccine patent acquisition
12 Winner of the Animal medicine manufacturer self- regulation checking system Prize
01 Anigen AIV Ab ELISA knowledge economics department new product certi�cation (NEP) acquisition
02 Name change of Animal genetics, Inc. to BioNote, Inc.
03 Moved to new Dongtan factory
05 Food & Drug Administration medicine manufacturing industry permission acquisition
12 ISO 2003:13485 TUV certi�cation acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed
11. 01 Acquisition by Alere
12. 02 Enlarged production facility and HQ
12. 04 Applied for International Patent for A Novel Canine In�uenza virus and Vaccine Therefore (PCT)
INTRODUCTIONBioNote, Inc. was established in the beginning of 2003, and is considered a pioneer of in-vitro Diagnostics for veterinary needs. BioNote delivers innovative and creative solutions for our customers improving their vet healthcare. BioNote has it's own automated facility to manufacture a wide range of high quality products developed by a highly competent R&D center. BioNote has manufactured diagnostic products in accordance with ISO 9001:ISO13485 certification, and has expanded overseas sales network in over 80 countries and is still continuing to grow.
If you want to know more about BIONOTE, please scan this QR-code on your mobile
03040506
07080911
03 Anigen, Inc. established
11 Acquired animal medicine import license
12 Acquired animal medicine manufacturing industry permission
09 Acquired ISO 9001:2000 TUV certi�cation
08 Moved to the new Young-tong factory
01 Purchased the site for the new Dongtan factory
04 Canine Distemper virus antibody diagnostic kit patent acquisition
04 Canine Parvovirus antibody diagnostic kit patent acquisition
06 Venture business certi�cation (No. 061627021-01133)
07 Technical innovation medium and small enterprises certi�cation (No.6065-1216)
07 Gyeonggi-do excellent medium and small enterprises selection
09 H5 and general pathogenic avian in�uenza virus antigen diagnostic
11 Name change of Anigen, Inc. to Animal Genetics, Inc.
12 Winner of the Animal medicine manufacturer self- regulation checking system Prize
02 World’s �rst In�uenza H3N2 type con�rmation assay development
07 Novel canine in�uenza viruses and vaccine patent acquisition
12 Winner of the Animal medicine manufacturer self- regulation checking system Prize
01 Anigen AIV Ab ELISA knowledge economics department new product certi�cation (NEP) acquisition
02 Name change of Animal genetics, Inc. to BioNote, Inc.
03 Moved to new Dongtan factory
05 Food & Drug Administration medicine manufacturing industry permission acquisition
12 ISO 2003:13485 TUV certi�cation acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed
11. 01 Acquisition by Alere
12. 02 Enlarged production facility and HQ
12. 04 Applied for International Patent for A Novel Canine In�uenza virus and Vaccine Therefore (PCT)
HISTORY
Purchased the site for the new Dongtan factoryCanine Distemper virus antibody diagnostic kit patent acquisitionCanine Parvovirus antibody diagnostic kit patent acquisition Venture business certification (No. 061627021-01133) Technical innovation medium and small enterprises certification (No.6065-1216)
World’s first Influenza H3N2 type confirmation assay developmentNovel canine influenza viruses and vaccine patent acquisitionWinner of the Animal medicine manufacturer selfregulation checking system Prize
Acquisition by Alere
Built the 2nd building for Manufacturing, Warehouse, R&D lab.Registered International Patent (PCT) for “A Novel Canine Influenza virus and Vaccine”
AniGen TB-feron ELISA Kit was assigned as an official diagnosis method for bovine tuberculosis eradication program in Korea
Spun off as an independent company from Alere, Inc. (USA)Expanded Manufacturing facility
Anigen, Inc. establishedAcquired animal medicine import licenseAcquired animal medicine manufacturing industry permission
Acquired ISO 9001:2000 TUV certification
Moved to the new Young-tong factory
Gyeonggi-do excellent medium and small enterprises selectionH5 and general pathogenic avian influenza virus antigen diagnosticName change of Anigen, Inc. to Animal Genetics, Inc.Winner of the Animal medicine manufacturer self- regulation checking system Prize
Anigen AIV Ab ELISA knowledge economics department new product certification (NEP) acquisitionName change of Animal genetics, Inc. to BioNote, Inc.Moved to new Dongtan factoryFo o d & D ru g Ad m i n i s t rat i o n m e d i c i n e manufacturing industry permission acquisitionISO 2003:13485 TUV certification acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed
2003. 032003. 112003. 12
2004. 09
2005. 08
2007. 07
2007.09
2007.11
2007. 12
2009. 01
2009. 02
2009. 032009. 05
2009. 12
2006. 012006. 04
2006. 04
2006. 06
2006. 07
2008. 02
2008. 07
2008. 12
2011. 01
2012. 02
2012. 04
2013. 12
2014. 11
2014. 12
2003
2006
2008
2007
2009
2011~2014
II RapidITest
05IAIVIAg06IH5IAIVIAg07IAIVIAb08INDVIAg09IIBVIAg
10IIBDVIAg
II ELISA
11IAIVIAbIELISA12IH5IAIVIAbIELISAI13INDVIAbIELISAI14IIBVIAbIELISA15IIBDVIAbIELISA
II PCRIKitsIforIAvianIdisease
AvianIproduct >>
Poultry Sampling Video Clip
Rapid Test Procedure Video Clip
* Please scan QR-codes for more information of poultry sampling and rapid test procedure
5
AIV Ag Influenza viruses that infect birds are called "Avian Influenza virus (AIV)" with only influenza type A viruses infecting birds. Influenza type A viruses are divided into subtypes based on two proteins, hemagglutinin (HA) and neuraminidase (NA), on the surface of the virus. AIV can infect chickens, turkeys, pheasants, quail, ducks, geese, and guinea fowl, as well as wide variety of birds. AIV is transmitted primarily through direct contact from infected birds to healthy birds, and through indirect contact with contaminated equipment and materials. The virus is excreted through infected birds' feces and secretions from their noses, mouths, and eyes.
Ordering Information
Test Procedures
•Field monitoring of Avian Influenza virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases
Indications
Special Features•Detection of all AIV type A•No cross-reaction with other avian viruses•Applicable to various species•World's first commercialized rapid test kit for AIV
C
C
T
2 1
S
AIV
Ag
Take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
1
2
3 4
Collect swab sample from trachea, kidney, spleen or cloaca(feces.)
Eye
Bronchial Tubes
Cloaca
Small Intestine
Mesentery
Large Intesline
Oviduct
KidneyOvaryCeca
Lungs
Duodenal Loop
Pancreas
Heart
EsophagusTrachea
Crop
proventriculusGall Bladder
SpleenLiver
Gizzard
OropharynxSuperior Larynx
Eye
Cloaca
Small Intestine
Oviduct
KidneyOvary
Lungs
Pancreas
Heart
Trachea
Crop
Gall Bladder
SpleenLiver
Gizzard
Insert the swab into the sample tube containing assay diluent, and mix the swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
20 min. C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Avian Influenza type A virus antigen
•Validated from OIE reference laboratories•Specimen: Trachea, kidney, spleen or cloaca(feces) •Sensitivity: 100% by farm (n=19), 77.3% by feces (n=150)•Specificity: 100% vs. HA, PCR (n=1,402)
Validated from *VLA, Veterinary Laboratory Agency, United Kingdom *FLI, Friedrich Loeffler Institute FLI, Germany *CSIRO, Australian Animal Health Laboratory *FGI «ARRIAH», Federal Center For Animal Health, Russia
Cat. No. Description Type Packing sizeRG15-01DG
Rapid AIV AgDevice 1 Test x 25/Kit
RG15-01MH Multi device 10 Tests x 3/Kit
Avi
an
6
H5 AIV Ag Avian influenza A viruses can be classified as low and high pathogenic form based on the severity of the illness they cause. Avian influenza A subtype H5 and subtype H7 viruses can be considered as "high pathogenic" strains on the basis of genetic features of the virus and the severity of the illness. Avian influenza A subtype H5 potentially has nine different subtypes (H5N1 ~ H5N9) and these subtypes can be highly pathogenic (HPAI) or low pathogenic (LPAI). The Influenza type A subtype H5 infection has been reported among humans and sometimes causes severe illness and death.
Ordering InformationCat. No. Description Type Packing size
RG15-05DGRapid H5 AIV Ag
Device 1 Test x 25/KitRG15-05MH Multi device 10 Tests x 3/Kit
Test Procedures
•Field monitoring of Avian Influenza virus subtype H5•Tentative diagnosis for swift control in outbreak suspected situation•Rule out low pathogenic AIV strain
Avian Influenza type A subtype 5 virus antigen
Indications
Special Features•H5 AIV detection only •No cross-reaction with other avian viruses•Applicable to various species•World's first commercialized rapid test kit for AIV H5•Validated from OIE reference laboratories
C
C
T
2 1
S
H5 A
IV A
g
Take the supernatant with disposable dropper provided.
Add 4 drops into the sample holewith disposable dropper.
4 drops
2
3 4
Collect swab sample from trachea, kidney, spleen or cloaca(feces.)
Eye
Bronchial Tubes
Cloaca
Small Intestine
Mesentery
Large Intesline
Oviduct
KidneyOvaryCeca
Lungs
Duodenal Loop
Pancreas
Heart
EsophagusTrachea
Crop
proventriculusGall Bladder
SpleenLiver
Gizzard
OropharynxSuperior Larynx
Eye
Cloaca
Small Intestine
Oviduct
KidneyOvary
Lungs
Pancreas
Heart
Trachea
Crop
Gall Bladder
SpleenLiver
Gizzard
1
Insert the swab into the sample tube containing assay diluent, and mix the swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
20 min.
•Specimen: Trachea, kidney, spleen or cloaca(feces) •Sensitivity: 100% by farm (n=13), 76.6% by feces
(n=115) vs. Virus isolation•Specificity: 100% vs. Virus isolation, PCR (n=1,402)
Validated from *FGI «ARRIAH», Federal Center For Animal Health, Russia *Giessen University, Gemany *IZS Umbria e Marche, Peruzia, Italy
H5
AIV
Ag
InterpretationC 2 1
Negative
C 2 1H5 AIV Positive
AIV Positive
C 2 1
Invalid
7
AIV Ab Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen Rapid AIV Ab Test Kit has been developed for screening these latent AIV carriers by detecting AIV antibodies.
Ordering InformationCat. No. Description Type Packing size
RB25-01MH Rapid AIV Ab Multi device 10Tests x 3/Kit
Test Procedures
•Avian influenza virus antibody detection•Field monitoring of Avian Influenza virus carrier•Antibody detection for migratory bird surveillance
Indications
Special Features•No cross-reaction with other avian virus positive sera•Applicable to various species•The world’s first rapid test kit for antibody to AIV
C TC 1 2
AIV Ab
Serum 1 1 drop of serum2 3 drops of assay diluent3
20 min.
C TC 1 2
AIV Ab
"Test result with 2 lines is a negative result and 1 line is a positive result."
Avian Influenza type A antibody
Anigen Rapid Avian Influenza Virus Antibody Test Kit has been validated against following antisera
H1N1 H2N3 H3N2 H3N8 H4N8 H5N1 H5N2H5N3 H6N2 H7N1 H7N3 H7N7 H8N4 H9N2H9N7 H10N1 H11N6 H12N5 H13N6 H14N2 H15N6
Performance characteristics•The detection limit of this kit is approximately HI titer 1:16•Serotype validation
•Specimen: Serum•Sensitivity: 96% vs. HI•Specificity: 96.5% vs. HI
Interpretation
Avi
an
8
NDV Ag Newcastle Disease (ND) is characterized by sneezing, coughing, and neurologic sign. Mortality rate is especially very high in the young age group (about 90%). Affected layer has poor egg quality and reduced egg production in initial phase but usually return to normal levels within four to eight weeks. ND can cause sudden death even in vaccinated poultry and spread rapidly to nearby flocks. Diagnosis is usually made by virus isolation, serology and clinical signs. It has been reported that hemagglutination inhibition (HI) test which is used widely in ND virus serology has cross reactions with other paramyxoviruses. The Anigen Rapid NDV Ag test kit is highly specific to NDV.
Ordering InformationCat. No. Description Type Packing size
RG15-03DD Rapid NDV Ag Device 1 Test x 10/kit
Test Procedures
•Field monitoring of Newcastle Disease virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases
Indications
Special Features•Detection of all NDV•No cross-reaction with other avian viruses •World's first commercialized rapid test kit for detection of NDV
Eye
Cloaca
Small Intestine
Oviduct
KidneyOvary
Lungs
Pancreas
Heart
Trachea
Crop
Gall Bladder
SpleenLiver
Gizzard
C
C
T
2 1
S
ND
V A
g
Take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
1
2
3 4
Collect swab sample from oropharynx, kidney or spleen.
4 drops
10 min.
Insert the swab into the sample tube containing assay diluent, and mix the swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Newcastle Disease virus antigen
•Specimens: Trachea, kidney or spleen•Sensitivity: 94.7% vs. RT-PCR•Specificity: 96.4% vs. RT-PCR
H5
AIV
Ag
9
IBV Ag Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all ages of chickens. The mortality rate is depending on the age and immune status of the birds when infected, and the presence of secondary invading organisms such as E. coli. It is highly contagious disease that can be transmitted through an entire flock within one or two days, through aerosol transmission (sneezing), contaminated organic material, drinking water and equipment. The target organs of the virus are trachea and kidney for the respiratory strain and nephrogenic strain respectively. It is financially important disease in poultry industry because affected layers have decreased egg production and poor egg quality.
Ordering InformationCat. No. Description Type Packing size
RG15-13DD Rapid IBV Ag Device 1Test x 10/Kit
Test Procedures
•Field monitoring of Infectious Bronchitis virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases
Indications
Special Features•Detection of all IBV•No cross-reaction with other avian viruses •World's first commercialized rapid test kit for IBV
C
C
T
2 1
S
IBV
Ag
Insert the swab into the sample tube containing assay diluent and mix the swab until the sample has been dissolved into the assay diluent and squeeze the swab against the well of tube and then discard it.
Take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
1
2
3 4
Collect swab sample from trachea, kidney or cloaca (feces).
4 drops
Eye
Cloaca
Small Intestine
Oviduct
KidneyOvary
Lungs
Pancreas
Heart
Trachea
Crop
Gall Bladder
SpleenLiver
Gizzard
10 min.C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Infectious bronchitis virus antigen
•Specimens: Trachea, kidney or cloaca(feces) •Sensitivity: 94.1% vs. RT-PCR•Specificity: 95.2% vs. RT-PCR
H5
AIV
Ag
Avi
an
10
IBDV Ag Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually affecting young chickens 3 to 6 weeks old, and transmitted by contaminated feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an important organ for young chickens as an immune development. IBDV serotype 1 causes clinical disease in chickens younger than 10 weeks, with older chickens usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is sometimes found in chickens and ducks. In practice, a diagnosis can be indicated by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and the presence of distinctive lesions in the Bursa of Fabricious and accompanying blood spots in the musculature of the breast and thigh of affected chickens
Ordering InformationCat. No. Description Type Packing size
RG15-04DD Rapid IBDV Ag Device 1 Test x 10/Kit
Test Procedures
•Field monitoring of Infectious Bursal Disease virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases
Indications
Special Features•Detection of all IBDV•No cross-reaction with other avian viruses •Specimen: Bursa of Fabricious, Cloaca
C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Infectious Bursal Disease virus antigen
•World's first commercialized rapid test kit for IBDV•Sensitivity: 99.9% vs. RT-PCR•Specificity: 96.6% vs. RT-PCR
C
C
T
2 1
S
IBD
V A
g
Insert the swab into the sample tube containing assay diluent and mix the swab until the sample has been dissolved into the assay diluent.
Take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
2
3 4Collect swab sample from Bursa of Fabricious or cloaca (feces)
Eye
Cloaca
Small Intestine
Oviduct
KidneyOvary
Lungs
Pancreas
Heart
Trachea
Crop
Gall Bladder
SpleenLiver
Gizzard
1
10 min.
H5
AIV
Ag
11
AIV Ab ELISA
Ordering InformationCat. No. Description Type Packing size
EB45-02PO AIV Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Avian influenza virus antibody detection•Monitoring of Avian Influenza virus carrier•Antibody detection for migratory bird surveillance
Indications
Special Features•No cross-reaction with other avian virus positive sera•Applicable to various species•Specimen: Serum, plasma or egg yolk•No sample dilution is required
1. Prepare AIV NP antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of anti AIV antibody-HRP to each well4. Incubate plate for 30 minutes at 37℃
5. Wash plate 6 times6. Add 100㎕ of substrate (Ready to use) and incubate the wells
for 10 minutes at room temperature7. Add 100㎕ of stopping solution8. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm9. PI value=[1-(OD sample/mean OD negative)] x 100
Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen AIV Ab ELISA has been developed for screening these latent AIV carriers by detecting AIV antibodies.
3 days
0 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
1.2
1
0.8
0.6
0.4
0.2
0
AniGen AIV ELISA
HI test
Starting point of Ab detection
Evaluation of AIV Antibody detection in egg yolk
*AniGen AIV Ab ELISA kit showed that it can detect the antibody in egg yolk approximately 3~5 days earlier than HI test.
Avian Influenza type A virus antibody
•Reading time: 45 minutes•Sensitivity: Chicken 98.2%, Duck 97.5%, Turkey 97.1%•Specificity: Chicken 97.3%, Duck 93.8%, Turkey 100%
Validated from *IZS delle Venezie, Legnaro (Padova), Italy *FGI «ARRIAH», Federal Center For Animal Health, Russia *University of Hokkaido, Graduated School of Veterinary Medicine
Avi
an
12
H5 AIV Ab ELISA
Ordering InformationCat. No. Description Type Packing size
EB45-03PO H5 AIV Ab ELISA Microplate 480 Wells/ Kit
Quick Procedure
•Antibody titer check after vaccination •H5 AIV carrier monitoring in non-vaccinated group •H5 AIV antibody detection for prevention program
Indications
Special Features•No cross-reaction with other AIV subtype antibodies •Species: Chicken, duck, quail•Specimen: Serum, Plasma•No sample dilution is required
1. Prepare AIV H5 antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of Mab-HRP (1:100 dilution in the conjugate diluent)
to all wells4. Incubate plate for 90 minutes at 37℃
5. Wash plate 6 times6. Add 100㎕ of substrate solution and incubate for 15 minutes
at room temperature7. Add 100㎕ of stop solution8. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm9. PI value= [1-(OD sample/mean OD negative)] x 100
Wild birds and some poultry infected by HPAI subtype H5 show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen H5 AIV Ab ELISA has been developed for screening these latent H5 AIV carriers by detecting AIV subtype H5 antibodies.
Serotype validation - AniGen H5 AIV Ab ELISA detects only H5 serotype- High sensitivity and specificity compared with HI test serotype validation
H1N1 H3N2 H2N9 H5N2 H5N1 H5N3 H7N7 H9N2
120
100
80
60
40
20
0
PI 50
Serotype validation
PI v
alue
Avian Influenza type A subtype H5 virus antibody
• Reading time: 1 hour and 45 minutes•Sensitivity: Chicken 96.9% (vs. HI)•Specificity: Chicken 100 % (vs. HI)
13
NDV Ab ELISA Newcastle disease is a contagious bird disease affecting many domestic and wild avian species. NDV strains can be categorized as velogenic (highly virulent), mesogenic (intermediate virulence) or lentogenic (non virulent). Velogenic strain is very contagious and produces severe nervous and respiratory signs and catastrophic (90% of mortality or more). Mesogenic strain causes cough, affect egg quality and production and results in about 10% of mortality. Lentogenic strains produce mild signs with negligible mortality. In acute cases, the death is very sudden, and, in the beginning of the outbreak, the remaining birds do not seem to be sick. In flock with good immunity, the signs (respiratory or gastrointestinal) are mild and progressive, and are followed after 7 days by nervous symptoms, especially twisted heads.
Ordering InformationCat. No. Description Type Packing size
EB45-06PO NDV Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Routine monitoring of NDV•NDV diagnosis of non-vaccinated group•Antibody titer check after vaccination
Indications
Special Features•Optimal screening method for NDV•Reading time: 75 minutes•Specimen: Serum
1. Prepare NDV antigen coated test plate2. Dilute test sample with sample diluent to 1:500
Do not dilute controls3. Add the 100㎕ of control sera and diluted test samples to
each well4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate and incubate for 30
minutes at room temperature7. Wash the wells 5 times8. Add 100㎕ of substrate solution to each well9. Incubate the wells for 15 minutes at room temperature10. Add 100㎕ of stopping solution to each well11. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm12. S/P ratio= [OD sample-mean OD negative] / [mean OD
positive- mean OD negative] x 100
Antibody development after vaccination and challengePositive rate was 100% after 3 weeks of vaccination in both ELISA (Shade zone)AniGen ELISA S/P ratio was generally higher than “I” commercial ELISA
Anigen
IDEXX
S/P
ratio
day0 day7 day14 day21 day28 day35 day42
Vaccination (IB,/ND) NDV challenge
8
7
6
5
4
3
2
1
0
-1
Newcastle Disease virus antibody
•Sensitivity: 98.4% vs. “I” commercial ELISA•Specificity: 97.7% vs. “I” commercial ELISA
Avi
an
14
IBV Ab ELISA Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all ages of chickens. The morbidity rate is extremely high and the mortality rate is dependent on the age of the chickens when infected, and the presence of secondary invading organisms such as E. coli. It is highly contagious disease that can be transmitted through an entire flock within one or two days, through aerosol transmission (sneezing), contaminated organic material, drinking water and equipment. The target organs of the virus are trachea and kidney for the respiratory strain and nephrogenic strain respectively. It is financially important disease in poultry industry because affected layers have decreased egg production and poor egg quality.
Ordering InformationCat. No. Description Type Packing size
EB45-05PO IBV Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Routine monitoring of IBV• IBV diagnosis of non-vaccinated group•Antibody titer check after vaccination
Indications
Special Features•Optimal screening method for IBV•Reading time: 75 minutes•Specimen: Serum
1. Prepare IBV antigen coated test plate2. Dilute test sample with sample diluent to 1:500
Do not dilute controls3. Add the 100㎕ of control sera and diluted test samples to
each well4. Incubate the wells at room temperature (18~25℃) for 30
minutes5. Wash the well at 5 times6. Add 100㎕ of enzyme conjugate to each well7. Incubate the wells at 18~25℃(Room Temperature) for 30
minutes and wash the wells 5 times8. Add 100㎕ of substrate solution to each well and incubate
the wells for 15±1 minutes at room temperature (18~25℃)9. Add 100㎕ of stopping solution10. Read the absorbance of the wells with a spectrophotometer
at 450nm with reference wavelength at 620nm11. S/P value=[OD samples - OD of negative control]/[OD
positive control – OD of negative control]
Positive rate after vaccinationAniGen IBV ELISA shows higher positive rate and S/P ratio than “B” ELISAMaternal antibody interference effect is shorter in Anigen than “B” ELISA (Shade zone)
0
20
40
60
80
100
WPI
Posi
tive
rate
WPI
S/P
ratio
3.5
3
2.5
2
1.5
1
0.5
0
Anigen IBV Ab ELISA
“B” IBV Ab ELISA
1 3 5 7 10 14 18 22
1 3 5 7 10 14 18 22
Infectious Bronchitis virus antibody
•Sensitivity: 98.8% vs. “I” commercial ELISA•Specificity: 97.8% vs. “I” commercial ELISA
15
IBDV Ab ELISA Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually affecting young chickens 3 to 6 weeks old, and transmitted by contaminated feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an important organ for young chickens as an immune development. IBDV serotype 1 causes clinical disease in chickens younger than 10 weeks, with older chickens usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is sometimes found in chickens and ducks. In practice, a diagnosis can be indicated by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and the presence of distinctive lesions in the Bursa of Fabricious and accompanying blood spots in the musculature of the breast and thigh of affected chickens
Ordering InformationCat. No. Description Type Packing size
EB45-07PO IBDV Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Routine monitoring of IBDV• IBV diagnosis of non-vaccinated group•Antibody titer check after vaccination
Indications
Special Features•Optimal screening method for IBDV•Reading time: 75 minutes•Specimen: Serum
1. Prepare IBV antigen coated test plate2. Dilute test sample with sample diluent to 1:500
Do not dilute controls3. Add the 100㎕ of control sera and diluetd test samples to
each well4. Incubate the wells at room temperature (18~25℃) for 30
minutes5. Wash the wells 5 times6. Add 100㎕ of enzyme conjugate to each well7. Incubate the wells at 18~25℃(Room Temperature) for 30
minutes and wash the wells 5 times8. Add 100㎕ of substrate solution to each well and incubate
the wells for 15±1 minutes at room temperature (18~25℃)9. Add 100㎕ of stopping solution10. Read the absorbance of the wells with a spectrophotometer
at 450nm with reference wavelength at 620nm11. S/P ratio=[OD450 samples - OD450 NCx] / [OD450 PCx -
OD450 NCx]
Infectious Bursal Disease virus antibody
•Sensitivity: 99.5% vs. “I” commercial ELISA•Specificity: 94.6% vs. “I” commercial ELISA
Antibody detection after vacciantion- AniGen IBDV Ab ELISA detects IBDV Ab 2weeks after vaccination
0.000
0.500
1.000
1.500
2.000
2.500
11(DPI-0)
17(DPI-0)
24(DPI-4)
27(DPI-7)
31(DPI-11)
34(DPI-14)
38(DPI-18)
41(DPI-21)
S/P
Age
BioNote
“I” commercial ELISA
0.2
Avi
an
16
PCR Kits for Avian disease
List of PCR Kits for Avian disease
•Confirm poultry diseases by antigen detection •Differential diagnosis of difficult case shows similar signs•Use to clarify signs of infection in vaccinated group showing uncertain antibody titer
Indications
Special Features•Ultimate diagnostic system for poultry infectious disease •Optimal and unique primers and probes system guarantees highly specific result•BioNote general PCR Detection Kits contain all components for the detection of agents• Includes positive and negative control•More convenient method than virus culture•One step PCR using Taqman probe (Real-Time)•Enables quantitative and qualitative analysis (Real-Time)•Higher detection limit than general PCR : 100~1,000 times more sensitive (Real-Time)
Product Diagnosis Cat. No.
General
AIV Detection Kit AIV type A (Matrix gene) PD55-01ON
AIV H5 Detection Kit Subtype H5 PD55-02ON
Velo NDV Detection Kit High pathogenic NDV virus (Velogenic) PD55-08ON
IBDV Detection Kit Infectious Bursal Disease virus PD55-11ON
Real-time
AIV A Real-Time Detection Kit AIV H5/N1 Duplex Detection Kit PD65-01ON
Velo NDV Real-Time Detection Kit High pathogenic NDV virus (Velogenic) PD65-08ON
IBV Real-Time Detection Kit Infectious Bronchitis Virus PD65-09ON
IBDV Real-Time Detection Kit Infectious Bursal Disease Virus PD65-11ON
MYCO G/S Real-Time Detection Kit Avian Mycoplasma gallisepticum, and M. iowae, M. meleagridis , M. synoviae PD65-14ON
II RapidITest
18IPEDIAg19ITGEIAg20IRotaIAg
II ELISA
21ICSFVIAbIELISA22IPRRSIAbIELISAI4.024IPEDIIgAIELISA25IPCV-2IAbIELSIA
II PCRIKitsIforISwineIdisease
SwineIproduct >>
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PED Ag Porcine epidemic diarrhea virus (PEDV) is a member of the coronavirus group which causes watery diarrhea, dehydration and high mortality in suckling pigs. Porcine Transmissible Gastroenteritis Virus (TGEV) is serologically unrelated with PEDV, but clinically these two virus infections are difficult to differentiate. The PEDV is transmitted by feces from infected pigs after oral ingestion. Current available laboratory diagnosis for PED is a cryostat section - direct immunofluorescence assay of the small intestine or colon. ELISA and RT-PCR assays to detect viral antigens in feces or intestinal contents are useful, but require specialized instruments and laboratory personnel. The Anigen Rapid PED Ag test kit has been developed to provide fast and reliable test results of PED antigen in field conditions.
Ordering InformationCat. No. Description Type Packing size
RG14-01DD Rapid PED Ag Device 1 Test x 10/KitRG14-05DD Rapid PED/Rota Ag Dual Device 1 Test x 10/Kit
Test Procedures
•Differential diagnosis of swine diarrheal disease •PEDV field monitoring•Tentative diagnosis for swift control in outbreak suspected situation
Indications
Specifications•No cross reaction with other etiologic agents•World's first & sole PED virus antigen rapid test kit•Specimen : Diarrheal feces•Sensitivity: 92% vs. RT-PCR•Specificity: 98% vs. RT-PCR
C
C
T
2 1
S
PE
D A
g
or
Collect sample from diarrheal feces using the swab.1
3 4
2
Wait 30 seconds for sedimentation and take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
Insert the swab into the sample tube containing assay diluent, and mixthe swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
5~10min.C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Porcine Epidemic Diarrhea virus antigen
H5
AIV
Ag
Not enough Good Too much
[Proper sample amount collected by a swab]
19
TGE Ag Transmissible gastroenteritis (TGE) is a viral disease of the small intestine that causes vomiting and diarrhea in pigs of all ages. The infection spreads rapidly by aerosol or contact exposure. Severe epidemics are more common during winter due to survival of the virus in colder temperatures. Depending on the level of immunity and exposure, diarrhea may be mild in some litters but severe in others. Because TGE virus is easily spread during an epidemic by persons, animals, and other means, fast diagnosis and special care should be taken to prevent spread to unexposed groups of pigs and to neighboring herds. Current clinical signs in the epidemic form of TGE usually justify a presumptive diagnosis. In the mild endemic form, laboratory confirmation is required. The Anigen Rapid TGE test kit has been developed to provide fast and reliable test results of TGE antigen in field conditions.
Ordering InformationCat. No. Description Type Packing size
RG14-02DD Rapid TGE Ag Device 1 Test x 10/KitRG14-03DD Rapid TGE/PED Ag Dual Device 1 Test x 10/Kit
Test Procedures
•Differential diagnosis of swine diarrheal disease •TGE field monitoring•Tentative diagnosis for swift control in outbreak suspected situation
Indications
Special Features•No cross reaction with other etiologic agents•World's first & sole TGE virus antigen rapid test kit•Specimen : Diarrheal feces•Sensitivity: 92.1% vs. RT-PCR•Specificity: 95.2% vs. RT-PCR
C
C
T
2 1
S
TGE
Ag
or
1
3 4
2
Wait 30 seconds for sedimentation and take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
Insert the swab into the sample tube containing assay diluent, and mixthe swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
Collect sample from diarrheal feces using the swab.
5~10min. C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Transmissible Gastroenteritis virus antigen
H5
AIV
Ag
Not enough Good Too much
[Proper sample amount collected by a swab]
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Rota Ag Rotavirus is the most common viral causative agent of diarrhea in pigs, cattle and dogs. Group A and B rotavirus are involved with group A being most prevalent and clinically important, containing several serotypes of differing virulence. All ages are susceptible. If neonatal pigs and cattle do not receive protective levels of maternal antibody, they are likely to develop profuse watery diarrhea in 12-48 hr. More commonly, the infection is endemic in a herd. Usually confirmatory diagnosis is based on histologic demonstration of villous atrophy in the jejunum or electron microscopy demonstration of virion in the intestinal contents. These methods take a long time and require high priced specialized equipment and expertise. The Anigen Rapid Rota Ag test kit has been developed to provide fast and reliable test results of Rota antigen in field conditions.
Ordering InformationCat. No. Description Type Packing size
RG18-03DD Rapid Rota Ag Device 1 Test x 10/KitRG14-05DD Rapid PED/Rota Ag Dual Device 1 Test x 10/Kit
Test Procedures
•Differential diagnosis of bovine and swine diarrheal disease •Rotavirus field monitoring•Tentative diagnosis for swift control in outbreak suspected situation
Indications
Specifications•No cross reaction with other etiologic agents•World's first commercialized rapid test kit for detection of
Rotavirus antigen•Specimen : Diarrheal feces•Sensitivity: 92% vs. RT-PCR•Specificity: 99% vs. RT-PCR
C
C
T
2 1
S
Rot
a A
g
or
1
3 4
2
Wait 30 seconds for sedimentation and take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
Insert the swab into the sample tube containing assay diluent, and mixthe swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
Collect sample from diarrheal feces using the swab.
5~10min.C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Rotavirus antigen
H5
AIV
Ag
Not enough Good Too much
[Proper sample amount collected by a swab]
21
CSFV Ab ELISA Classical Swine Fever Virus (CSFV), previously called hog cholera virus, is a Pestivirus in the family Flaviviridae. The CSFV is closely related to the ruminant pestiviruses which cause Bovine Viral Diarrhea (BVDV) and Border Disease (BDV). The virulence of CSFV strains varies widely which is leading to a wide range of clinical signs. Highly virulent strains result in acute and severe clinical signs including neurological signs and hemorrhages and high mortality rate. Less virulent strains can give rise to sub-acute or chronic infections that may escape detection, while still causing abortions and stillbirth. Herds in high risk areas are usually serologically tested on a thorough statistical basis.
Ordering InformationCat. No. Description Type Packing size
EB44-13PO CSFV Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Routine monitoring of CSFV•Diagnosis of CSFV by antibody titration •Titer check after vaccination
Indications
Special Features•No cross reaction with other swine disease positive sera•Blocking ELISA which is OIE reference method for CSFV •Specimen: Plasma, Serum•Reading time: 1 hour and 45 minutes•Sensitivity: 99.3% vs. Commercial ELISA•Specificity: 99.7 % vs. Commercial ELISA
1. Prepare CSFV antigen coated test plate2. Add 50㎕ of the sample diluent into each well of the plate 3. Add 50㎕ of controls and samples to wells4. Incubate plate for 60 minutes at 37℃
5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate(ready to use) into each well 7. Incubate the wells at 37℃ for 30 minutes 8. Wash plate 5 times 9. Add 100㎕ of substrate(ready to use) to each well and incubate for 15 minutes at room temperature10. Add 100㎕ of stopping solution 11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm12. PI value = [1-(mean OD sample/mean OD negative control)] x 100
Correlation rates with other commercial ELISA- Random 274 samples from vaccinated group
'P' ELISA 'I' ELISAAniGen CSFV Ab
ELISADifferent results
17 samples 27 samplesCorrelation rate 93.8% 90.0%
Classical Swine Fever virus antibody
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PRRS Ab ELISA 4.0 Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of piglets and growing pigs. The disease is caused by PRRS virus, currently classified as a member of family Arteriviridae, genus Arterivirus. The primary target cell of the virus is alveolar macrophage of pigs. Two major types of the virus exist, the European (EU) and the North America (NA) strain. The virus is primarily transmitted by contaminated feces, urine, semen and infected pigs. High level of sanitary management system is required because fomite infection is also possible.
Quick Procedure
•Routine monitoring of PRRS•PRRS diagnosis of non-vaccinated group•Titer check after vaccination
Indications
Special Features•No singleton reactor (false positive)•Antibodies against European strain, North America
strain and Korean strain can be detected•No cross reaction with other swine disease positive sera
1. Prepara antigen coated micro assay plate2. Dilute test samples and controls with sample diluent (1:39 dilution)3. Add 100㎕ of diluted samples and controls to wells4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate to wells7. Incubate plate for 30 minutes at room temperature8. Wash plate 5 times9. Add 100㎕ of substrate solution and incubate for 15 minutes at room temperature in dark10. Add 100㎕ of stop solution11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm12. S/P value=[OD sample-mean OD negative]/[ mean OD positive- mean OD negative]
Sensitivity and specificity study-AnGen PRRS Ab ELISA 4.0 has high sensitivity, 98.7% and high Specificity, 99.7%
AniGen PRRS Ab ELISA 4.0Positive Negative Total
IFAPositive 324 4 328Negative 1 333 334
Total 325 337 662
Porcine Reproductive and Respiratory Syndrome antibody
•Specimen: Plasma, Serum•Reading time: 75 minutes•Sensitivity: 98.7% vs. IFA•Specificity: 99.7% vs. IFA
23
Ordering InformationCat. No. Description Type Packing size
EB44-04PO PRRS Ab ELISA 4.0 Microplate 480 Wells/Kit
Seroconversion study after challenge of PRRSV
Compare with other commercial ELISAProduct AniGen “I” Commercial vol.2 “I” Commercial vol.3
Test well No. for 1 sample 1 Well 2 Well 1 Well
Time Incubation 30 min. Incubation 30 min. Incubation 30 min.Cut off value S/P 0.4 S/P 0.4 S/P 0.4
Singleton reactor No Yes No
AniGen PRRS Ab ELISA Kit is more sensitive than othersDetection period is earlier than "I" commercial ELISA with no singleton reactor
S/P
ratio
4.0
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0.0
0 7 13 18 24 29 35 40
AniGen
Commercial Kit A
S/P
ratio
4.0
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0.0
0 7 13 18 24 29 35 40
AniGen
Commercial Kit A
EU NA
cut off0.4
cut off0.4
* Anigen PRRS Ab ELISA 4.0 can detect PRRS Ab from DPI 7 with PRRS EU and NA strain
-1.0
0
1.0
2.0
3.0
4.0
AniGen
“I” Commercial vol.3
D0
Mea
n S
/P ra
tio
“I” Commercial vol.2
D14 D35
Days post vaccination
<PRRS vaccination DPI results>
1) Antibody response after challenge with PRRS EU, NA strainr
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PED IgA ELISA Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae. PEDV causes acute enteritis in swine of all ages, and it is often fatal for neonatal piglets. To protect piglets from PEDV, Sow transfers immunoglobulin through colostrum to their children until the piglets acquire adaptive immunity. Many reports suggest that IgA is important for protection of PEDV. AniGen IgA ELISA measures preventive anti PED-virus IgA titers of sow and predict defense capability of piglets induced by passive antibody transfer.
Ordering InformationCat. No. Description Type Packing size
EB44-10PO PED IgA Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Quantitative detection of PED IgA antibody•Screening for defensive capacity against PEDV
Indications
Special Features•Easy sample collection•Optimal screening method for defensive capacity of PEDV•Specimen : Colostrum
1. Prepare PED antigen coated test plate2. Dispense 100㎕ of sample diluent into each well3. Dispense 10㎕ of positive, negative control and samples to
each well4. Incubate the plate at 37±1℃ for 60 minutes5. Wash the plate 5 times6. Dispense 100㎕ of diluted enzyme conjugate to each well7. Incubate the wells for 30 minutes at37±1℃
8. Wash the plate 5 times9. Dispense 100㎕ of substrate to each well10. Incubate the wells for 15 minutes at room temperature
(18~25℃)11. Dispense 100㎕ of stopping solution12. Measure the optical density (OD) at 450nm with reference
wavelength at 620nm13. Cut off value = [0.35+ the mean Negative Control absorbance]
Changes in composition of colostrum
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
Colostrum7 14 21 28
Day
Conc
entr
atio
n
IgA
IgG
(The concentration of the IgA is higher than IgG in sow’s milk)
90
80
70
60
50
40
30
20
10
0
Oral IM Control
(Piglet survival rate)(Research in Veterinary Scien ce, Song et al., 2007)
Survival rate of P.O group (IgA)A-farm-OB-farm-O
Survival rate of I.M group (IgG)A-farm-IMB-farm-IM
◈ Inoculated agent : cell adapted DR13 strain◈ Speci�c ELISA was used for IgA detection
Survival rate(%)
Antibody development (in pregnant sows. 2007 research)
Porcine Epidemic Diarrhea virus
•Reading Time : 1 hour and 45 minutes•Survival rate of IgA positive confirmed group
after challenge : see tables below
25
PCV-2 Ab ELISA Porcine Circovirus Type 2, or PCV2, is a very small circular-arranged DNA virus that belongs to the Circoviridae family. PMWS is a serious manifestation of PCV2 infection. PMWS is characterized by severe loss of weight (wasting) and generalized lymph node enlargement. Today we know that there are several PCV2 associated diseases (PCVAD) and severe systemic PCV2 infection remains as the most important manifestation. PCV2-associated Porcine Respiratory Disease Complex (PRDC) is also a very commonly diagnosed PCVAD in the U.S. Less commonly diagnosed diseases include PCV2-associated Enteritis, PCV2- associated Reproductive Failure, and Porcine Dermatitis and Nephropathy Syndrome (PDNS). PCV2-infection is widespread and essentially all pig herds are infected with PCV2 but relatively few have PCVAD. In many cases, PCV2 infection requires a trigger such as co-infection with other pathogens (PRRSV, Mycoplasma hyopneumoniae), immune stimulation of the host, or other stressors to trigger PCV2 infection to progress to PCVAD. Host genetics may also markedly affect the outcome of PCV2 infection and there is increasing evidence of differences in virulence among PCV2 isolates.
Ordering InformationCat. No. Description Type Packing size
EB44-17PO PCV 2 Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Titer check after vaccination•Diagnosis of PCV2 by antibody titration•For prevention of PRDC, PDNS, PMWS
Indications
Special Features•Useful massive screening method for PCV2 •No cross reaction with other swine disease positive sera•Measurement of IgG induced by ORF 2 of PCV2 to predict
outcome of infection
1. Prepare antigen coated micro assay plate2. Dilute test samples and controls with sample diluent
(1:39 dilution)3. Add 100㎕ of diluted samples and controls to wells4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate to wells7. Incubate plate for 30 minutes at room temperature8. Wash plate 5 times9. Add 100㎕ of substrate solution and incubate for
15 minutes at room temperature in dark10. Add 100㎕ of stop solution11. Measure the optical density (OD) at 450 nm with
reference wavelength at 620nm12. S/P value = [OD sample-mean OD negative] / [ mean
OD positive- mean OD negative] x 100
0 7 14 21 49 69
VNT5
0 (lo
g 2)
11.5
10.5
9.5
8.5
7.5
6.5
5.5
4.5
1.2
1.0
0.8
0.6
0.4
0.2
0
Opt
ical
den
sity
Days post infection
12.0
10.0
8.0
6.0
4.0
2.0
0.0
6
5
4
3
2
1
00 7 14 21 49 69
VNT5
0 (lo
g 2)
Days p.i.
DN
A c
opie
s/m
l (lo
g 0)Correlation of neutralizing antibody titer with IgG and IgM-Neutralizing antibody (NA) titer was correlated with IgG
*NA: Green line, IgG: Red line, IgM: Blue line (Veterinary Microbiology 125 (2007) 244–255)
Dynamics and viral loads in PCV inoculated pigs-NA has inverse proportion relation with viral load
*NA: Green line, Viral load: Ivory bar (Veterinary Microbiology 125 (2007) 244–255)
•Specimen: Plasma, Serum•Reading time: 75 minutes
Porcine Circovirus 2 antibody
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PCR Kits for Swine disease
Real-Time PCR Kits for Swine disease
•Confirm swine diseases by antigen detection •Differential diagnosis of difficult case shows similar signs•To clarify signs of infection in vaccinated group showing uncertain antibody titer
Indications
Special Features•Ultimate diagnostic system for swine infectious disease •Optimal and unique primers and probes system guarantees highly specific result• Includes positive and negative control•More convenient method than virus culture•One-step PCR using Taqman probe•Enables quantitative and qualitative analysis •Higher detection limit than general PCR : 100~1,000 times more sensitive
Product Diagnosis Cat. No.
Real-Time
PCV-2 Real-Time Detection Kit Porcine Circovirus PD64-03ON
PRRSV Real-Time Detection Kit Porcine Reproductive and Respiratory SyndromeVirus (Differential diagnosis of EU/US strain) PD64-04ON
CSFV Real-Time Detection Kit Classical swine fever virus PD64-05ONInfluenza A (H1N1) Swine Real-Time Detection Kit Novel Influenza A (H1N1) PD64-01ON
SIV Real-Time Detection Kit Original Swine Influenza virus PD64-02ON
•BioNote *PRRS Real-Time Detection Kit →UsefultodifferentiateEUandPRRSVstrain
•BioNote *SIV Real –Time Detection Kit →UsefultodifferentiateSwineInfluenzaVirus(InfluenzaAH1N1)andoriginalSwineInfluenzavirus
Differential Diagnosis
BovineIproduct >>
II RapidITest
28IB.BrucellaIAb29IGS.BrucellaIAb30IBovineITBIAb31IFMDINSPIAb32IBoviD-5IAg
II ELISA
33IB.BrucellaIAbIELISAI2.034ITBIferonIELISA36IBTBIAbIELISAI2.037IFMDINSPIAbIELISA
Bovi
ne
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Validated from *IZS Umbria e Marche, Peruzia, Italy*Agence Française de Sécurité Sanitaire des Aliments, FranceB.Brucella Ab
Bovine brucellosis is commonly caused by B. abortus and less frequently by B. melitensis, and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Available serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. However, these tests require sample preparation or specialized person and equipment. The Anigen B. Brucella Ab Test Kit provides swift and accurate field test results.
Ordering InformationCat. No. Description Type Packing size
RG23-01DD Rapid B.brucella Ab Device 1 Test x 10/Kit
Test Procedures
•Diagnosis of bovine brucellosis in field•Screening for test and slaughter government policy •Massive screening for animal import & export
Indications
Special Features• Detection of antibodies against Brucella abortus,
melintensis and suis•Standardized by OIE standard sera (B.abortus 1119-3)•More reliable result than rose bengal test•High correlation with ELISA test
C T S
C 2 1B.br
ucell
a Ab
C T S
C 2 1B.br
ucell
a Ab
Slowly add one drop (20㎕) by a capillary tube of sample to the sample well.
Add 3 drops with the bottlecontaining Assay diluent.
3 drops20㎕
Serum, plasma 2 31
Whole blood20 min.
C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Bovine Brucellosis antibody
•World’s first commercialized rapid test kit for detection of B. brucellosis
•Specimen: Blood, Plasma, Serum •Sensitivity: 100% vs. ELISA•Specificity: 99.1% vs. ELISA
Advantages of Anigen Rapid B.Brucella Ab testRBT MRT Anigen
Specimen Serum only Milk only Whole blood, Serum, Plasma Use in field No Yes Yes
Time One day 1 hour 20 minutesCross reaction Cross reaction with Yersinia enterocolitica No cross reaction
* RBT : Rose Bengal Test* MRT : Milk Ring Test
H5
AIV
Ag
29
C T S
C 2 1GS.br
ucell
a AbC T S
C 2 1GS.br
ucell
a Ab
Slowly add one drop (20㎕) by a capillary tube of sample to the sample well.
Add 3 drops with the bottlecontaining Assay diluent.
3 drops20㎕
Serum, plasma 2 31
Whole blood20 min.
GS.Brucella Ab Ovine and caprine brucellosis is commonly caused by B. melitensis and less frequently by B. abortus and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Available serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. However, these tests require sample preparation or specialized person and equipment. The Anigen GS. Brucella Ab Test Kit provides swift and accurate field test results.
Ordering InformationCat. No. Description Type Packing size
RB23-06DD Rapid GS.brucella Ab Device 1 Test x 10/Kit
Test Procedures
•Diagnosis of ovine and caprine brucellosis in field•Screening for test and slaughter government policy •Massive screening for animal import & export
Indications
Special Features•Detection of antibodies against Brucella melintensis,
abortus and suis•More reliable result than rose bengal test•High correlation with ELISA test•World’s first commercialized rapid test kit for detection of
GS. brucellosis
C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Goat & Sheep Brucellosis antibody
•Specimen: Blood, Plasma, Serum, Milk•Sensitivity: 98% vs. ELISA•Specificity: 100% vs. ELISA
Advantages of Anigen Rapid GS.Brucella Ab testRBT MRT Anigen
Specimen Serum only Milk only Whole blood, Serum, Plasma Use in field No Yes Yes
Time One day 1 hour 20 minutesCross reaction Cross reaction with Yersinia enterocolitica No cross reaction
* RBT : Rose Bengal Test* MRT : Milk Ring Test
H5
AIV
Ag
Bovi
ne
30
Bovine TB Ab Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the basis of delayed hypersensitivity reactions. Subclinical infection is common and clinical signs are non-specific in many cases. A sensitive and accurate diagnostic tool is required because purified protein derivative (PPD) test has only a 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other mycobacterium infection (eg; M.avium) should be distinguished with this method. The MPB 70 protein was revealed to be a highly species specific protein secreted by Mycobacterium bovis. Anigen rapid bovine TB Ab test kit has been developed using MPB70 protein with a immunochromatographic assay method.
Ordering InformationCat. No. Description Type Packing size
RB23-02DD Rapid Bovine TB Ab Device 1 Test x 10/Kit
Test Procedures
•Search out BTB carrier in field•Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-
handed situation• CombinewithPPDorIFN-γassayineradicationsystem
Indications
Special Features•No cross reaction against other mycobacterium species (M. avium)• Easy to use, saving time and labor• Specimen: Blood, Plasma, Serum• Sensitivity: 81.7% vs. PPD test, 84.2% vs. “E” commercial ELISA• Specificity: 91.4% vs. PPD test, 84% vs. “E” commercial ELISA
BTB
Ab
S
C T
BTB
Ab
S
C T
Add 1 drop of the mixed sample and wait for another 1 minute.
Add 3 drops of developing buffer.
3 drops
Whole blood
4 5
3 drops 1 drop+
Add 3 drops of whole blooddiluent into the test tube.
2 Add 1 drop of whole blood into the test tube and wait for 1 minutes to mix it.
3
Plasma or serum
1 drop (10㎕)Whole blood : Start from Step 2Plasma or serum : Start from Step 4
1
1 min.
20 min.C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Mycobacterium bovis antibody
Imm
une
Resp
onse
Tuberculin Skin TestAntibody Response
Gamma InterferonTest (IFN-γ)
Aner
gy
AniGen TB feron ELISA Test Kit
PPD Test
Anigen Rapid Bovine TB Ab Test Kit
Anigen Rapid BTB Ab Test Kit detects mid to late (anergy) stage of TB affected cattle by immunochromatographic assay (Applicable in field assay)Generally, M.bovis Ab is detectable 90 to 100 days after infection
Immune development of bovine tuberculosis
31
FMD NSP Ab Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer, and water buffalo. In many countries the disease is controlled by vaccinations that consist of (partly) purified structural proteins (SP) of the FMD virus, and therefore vaccinated animals only elicit antibodies directed against the structural proteins. Non structural protein (NSP) is expressed only by replicating viruses, and inactivated vaccines are purified to remove the cellular proteins and NSP. Therefore, only animals that have been infected with wild type develop antibodies against NSP. It is important to differentiate SP and NSP antibodies in countries that use vaccination to control FMDV outbreaks to discriminate wild type infections and immune response to vaccination.
Ordering InformationCat. No. Description Type Packing size
RB28-02DD Rapid FMD NSP Ab Device 1 Test x 10/Kit
Test Procedures
•To discriminate between infection and vaccination•For field diagnosis of FMD in non-vaccinated herds•Tentative diagnosis for swift control in outbreak suspected situation
Indications
Special Features•No cross reaction with vaccinated group•Specimens: Blood, Plasma, Serum•Applicable to all artiodactyl mammals (Cattle, Sheep, Goat, Pig)•Sensitivity: 95.4% vs. Commercial ELISA•Specificity: 100% vs. Commercial ELISA
FMD
NSP
Ab
S
C T
FMD
NSP
Ab
S
C T
Add 1 drop of the mixed sample and wait for another 1 minute.
Add 3 drops of developing buffer.
3 drops
Whole blood
4 5
3 drops 1 drop+
Add 3 drops of whole blooddiluent into the test tube.
2 Add 1 drop of whole blood into the test tube and wait for 1 minutes to mix it.
3
Plasma or serum
1 drop (10㎕)Whole blood : Start from Step 2Plasma or serum : Start from Step 4
1
1 min.
15 min.
C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Food and mouth disease virus antibody
Bovi
ne
32
BoviD-5 Ag
Newborn calves are susceptible to neonatal calf diarrhea especially during their first 3~4 weeks of life. Bacteria, viruses and parasites, by attacking the lining of the calf`s intestine, give rise to diarrhea. It is one of the major financial damage factors in bovine farms, because once infected this decreases the absorption of essential nutrients from milk and leads to weight loss and dehydration. Generally, calf diarrhea is considered as an emergency situation that requires swift diagnosis and rehydration. One of the key features of a rapid test kit is the "rapidity" of the test result, thus BoviD- 5 Ag is an ideal test in these situations.
Ordering Information
Test Procedures
•For differential diagnosis of calf diarrhea•Calf diarrhea etiologic agent monitoring in field•For immediate treatment required cases
Indications
Special Features•Useful tool for ruling out the cause of calf diarrhea•Specimen : Diarrheal feces•No cross reaction with other etiologic agents of calf diarrhea•Sensitivity and Specificity : see table
Giardia Ag
or
Collect the samples from canine feces using the swab.1
4 5
2
Wait 30 seconds for sedimentation and take the supernatant with disposable dropper provided.
Add 4 drops into the sample hole with disposable dropper.
4 drops
Insert the swab into the sample tube containing assay diluent, and mixthe swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.
5~10min. C T
C T
C T
C T
Negative
Positive
Invalid
Interpretation
Bovine Rota, Corona, E.coli K99, Cryptosporidium, Giardia antigen
Not enough Good Too much
[Proper sample amount collected by a swab]
Sensitivity SpecificityCryptosporidium 98.2% 99.0%
vs.PCRRotavirus 99.0% 98.0%
Coronavirus 98.4% 98.0%E.coli K 99 97.8% 99.0%
Giardia 92.1% 99.1% vs.ELISA
Cat. No. Description Type Packing sizeRG13-02DD Rapid BoviD-5 Ag Device 1 Test x 10/KitRG13-01DD Rapid BoviD-4 Ag Device 1 Test x 10/Kit
33
B.Brucella Ab ELISA 2.0 Bovine brucellosis is commonly caused by Brucella abortus and less frequently by B. melitensis, and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. Anigen B.brucella Ab ELISA Test Kit is a serology test that provides high sensitivity and specificity with bovine milk and serum samples.
Ordering InformationCat. No. Description Type Packing size
EB43-01PO B.brucella Ab ELISA 2.0 Microplate 480 Wells/Kit
Quick Procedure
•Diagnosis of bovine brucellosis in lab•Screening for test and slaughter government policy •Massive screening for bovine import & export market
Indications
Special Features•Fully meets the requirement of EU Council Directive and the
OIE Manual •Standardized by OIE standard sera (B.abortus 1119-3)•No cross reaction with yersinia enterocolitica•Specimen: plasma, serum, milk•Reading time: 1 hour and 45 minutes•Sensitivity: serum 100%, milk 100% (Vs Commercial ELISA)•Specificity: Serum 97.9 %, milk 99.1% (Vs Commercial ELISA)
1. Prepare LPS coated test plate2. Dilute test samples 1:50 with sample diluent
(Do not dilute controls and Milk)3. Add 100㎕ of positive control, negative control, and diluted
test samples4. Incubate the plate for 60 minutes at 37℃
5. Wash plate 5 times6. Add 100㎕ of diluted enzyme conjugate to wells7. Incubate plate for 30minutes at 37℃
8. Wash plate 5 times9. Add 100㎕ of mixed substrate (Ready to use) to each well and
incubate for 15 minutes at room temperature10. Add 100㎕ of stopping solution11. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm12. %P value=[OD sample/mean OD positive] x 100
(1)Study in serum Specimens :Total 271 of positive and negative cow serum from KNVRQS*
AniGen ELISA + -
Other commercial ELISA
+ 127 0 127- 3 141 144
130 141
Result
Sensitivity 100%Specificity 97.9%
(2) Study in raw milk Specimens :Total 271 of positive and negative cow milk from KNVRQS*
AniGen ELISA + -
Other commercial ELISA
+ 68 0 68- 1 116 117
69 116
Result
Sensitivity 100%Specificity 99.1%
Bovine Brucellosis antibody
Bovi
ne
34
TB feron ELISA Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. The infection is often subclinical; even when present, clinical signs are not specifically distinctive of this disease and might include weakness, anorexia, emaciation, enlargement of lymphnodes, and cough. Routine screening can be time and labor intensive. The Anigen TB-feron is an indirect Enzyme Linked ImmunosorbentAssayforthequantitativedetectionofinterferongamma(IFN-γ).IFN-γassayisbasedonthefactthatananimalhasbloodlymphocyteswhichcanmemorize stimulating antigen immunologically when it is stimulated by exogenous or endogenous antigens. When an antigen is added to the blood from a primed animal within a tube, antigen specific effector/memory T cell are rapidly re-stimulatedtoproduceIFN-γ,thecytokine,whichisusedasaspecificmarker incell-mediated immune response (recall response). It is an alternative test method of intradermal skin test (PPD) because to its easy procedure and better sensitivity compared to the skin test.
Quick Procedure
•Quantitative detection of interferon gamma in bovine plasma
•Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-
handed situation•Combine with ELISA or Rapid test in eradication system
Indications Special Features•High reliable result than PPD•OIE standard for BTB diagnosis•GoodcorrelationwithotherIFN-γassay•Provide antigens for sensitization•Specimen : Stimulated plasma•Reading time : 2 hours •Concordance rate: 100% vs. “B” commercial ELISA
1. Add 100㎕ of sample diluent to each well2. Add 50㎕ of controls and each of prepared samples
(Bovine PPD stimulated plasma Avian PPD stimulated plasma, PBS stimulated plasma) to each well3. Incubate the wells at 37℃ for 30 minutes4. Wash the wells 5 times5. Add 100㎕ of prepared amplification conjugate to each well6. Repeat step 3 and 47. Add 100㎕ of prepared enzyme conjugate to each well8. Repeat step 3 and 49. Add 100㎕ of substrate to each well10. Incubate the wells for 30 minutes at room temperature11. Add 100㎕ of stopping solution to each well. Reading W/L 450nm (Ref. W/L 620nm)12. OD value: OD bovine PPD-PBS and OD bovine PPD-avian PPD
Sample preparation
Stimulating antigen (Sensitization should be performed within 30 hours after blood collection)100㎕- Bovine PPD- Avian PPD- PBS (Negative control)Plasma 3ml
Overnightincubation
for 16 hoursCentrifuge
Take supernatant
liquid
TB feronELISA
testing+
Gamma interferon assay for Mycobacterium bovis
35
Field benefits of the IFN-γ assay•Animals retested as often as required (no interference with the immune status of animal)•Double handing of cattle avoided•Better sensitivity compared to skin test•Reduced need for comparative intradermal test since both avian and bovine PPDs are used
Lab benefits of the IFN-γ assay•Results obtained within 24 hours•A lab test is subject to quality control, standard procedures and objective interpretation•Suitably adapted to epidemiological characteristics of the territory
Performance1. Comparative study with other IFN test
AniGen TB feronTotal
(-) (+)
"B" ELISA(-) 87 0 87 Correlation 100% 142/167(+) 0 12 12 Sensitivity 100% 12/12
Total 87 12 99 Specificity 100% 87/87
2. Comparative study with skin test (PPD)
AniGen TB feronTotal
(-) (+)
PPD(-) 50 0 50 Correlation 85% 142/167(+) 25 92 117 Sensitivity 78.6% 92/117
Total 75 92 167 Specificity 100% 50/50
Ordering InformationCat. No. Description Type Pack ingsize
EG38-01PO TB feron ELISA Microplate 480 Wells/Kit (150 tests)
Imm
une
Resp
onse
Tuberculin Skin TestAntibody Response
Gamma InterferonTest (IFN-γ)
Aner
gy
AniGen TB feron ELISA Test Kit
AniGen BTB Ab ELISA 2.0 Test Kit
PPD Test
AniGen TB feron ELISA detects early to mid stage of TB affected cattle by cellular immunity measurement
Immune development of bovine tuberculosis
Bovi
ne
36
BTB Ab ELISA 2.0 Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the basis of delayed hypersensitivity reactions. Subclinical infection is common and clinical signs are non-specific in many cases. A sensitive and accurate diagnostic tool is required because purified protein derivative (PPD) tests have been known to have 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other mycobacterium infection (eg; M.avium) should be distinguished with this method. The MPB 70 protein has been revealed to be a highly species specific protein secreted by Mycobacterium bovis. Anigen BTB Ab ELISA 2.0 test kit has been developed using MPB70 protein in an Enzyme Linked Immunosorbent Assay method. The serological diagnostic test is suitable for massive screening of bovine tuberculosis to detect mid to late (anergy) state cattle.
Ordering InformationCat. No. Description Type Packing size
EB43-04PO BTB Ab ELISA 2.0 Microplate 480 Wells/Kit
Quick Procedure
•Search out BTB carrier •Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-handed situation• CombinewithPPDorIFN-γassayineradicationsystem
Indications
Special Features•No cross reaction against other mycobacterium species (M. avium)•Quantitative detection of M. bovis antibody•The world’s first reliable BTB Ab ELISA for serological diagnosis
1. Prepare BTB antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of M. bovis antigen-HRP to each well 4. Incubate plate for 60 minutes at 37℃
5. Wash plate 6 times6. Add 100㎕ of mixed substrate solution (Ready to use)
to each well and incubate for 15 minutes at room temperature
7. Add 100㎕ of stopping solution to each well 8. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm9. S/P value=[OD sample-mean OD negative)] / [mean OD
positive – mean OD negative] x 100
Imm
une
Resp
onse
Tuberculin Skin TestAntibody Response
Gamma InterferonTest (IFN-γ)
Aner
gy
AniGen TB feron ELISA Test Kit
AniGen BTB Ab ELISA 2.0 Test Kit
PPD Test
AniGen BTB Ab ELISA 2.0 detects mid to late (anergy) stage of TB affected cattle by antibody titer check againt TB (Laboratory method for antibody check)Generally, M.bovis Ab is detectable 90 to 100 days after infection
Mycobacterium bovis antibody
•Reading time: 75 minutes•Specimen: Serum•Sensitivity: 88.1% vs. PPD test•Specificity: 99.2% vs. PPD test
Immune development of bovine tuberculosis
37
FMD NSP Ab ELISA Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer, and water buffalo. In many countries the disease is controlled by vaccinations that consist of (partly) purified structural proteins (SP) of the FMD virus, and therefore vaccinated animals only elicit antibodies directed against the structural proteins. Non structural protein (NSP) is expressed only by replicating viruses, and inactivated vaccines are purified to remove the cellular proteins and NSP. Therefore, only animals that have been infected with wild type develop antibodies against NSP. It is important to differentiate SP and NSP antibodies in countries that use vaccination to control FMDV outbreaks to discriminate wild type infections and immune response to vaccination.
Ordering InformationCat. No. Description Type Packing size
EB48-01PO FMD NSP Ab ELISA Microplate 480 Wells/Kit
Quick Procedure
•Discriminate sera between infection and vaccination•Diagnosis of FMD in non-vaccinated herds•Screening for test and slaughter government policy
Indications
Special Features•Differential test of FMD infected or vaccinated•High accuracy equivalent to a world standard ELISA kit•Easy test procedure: No serum pre-dilution required•Cost effective: No requirement for an uncoated
microplate for serum pre-dilution
1. Prepare FMD NSP antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of diluted enzyme conjugate to wells4. Incubate plate for 90 minutes at 37℃
5. Wash plate 6 times6. Dispense100㎕ of substrate (Ready to use) into each well
and incubate for 15 minutes at room temperature7. Add 100㎕ of stopping solution8. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm9. PI value=[1-(OD sample/mean OD negative)] x 100
Reactivity of AniGen FMD NSP Ab ELISA for sera originated from vaccinated animals
AniGen FMD NSP Ab ELISA detects antibodies against nonstructural protein from 7 days to 7 months after infection(*Experimentally contact challenge animal group, The performance evaluation was performed in OIE FMD Ref. Laboratory)
AniGen 0% 76.1% 93.8% 50%Checkit 0% 38.3% 93.8% 20%
0
20
40
60
80
100
AniGen
Checkit
5 days 7 days 14~26 days 7 months
Food and mouth disease virus antibody
Validated from *FGI «ARRIAH», Federal Center For Animal Health, Russia
•Species: Cattle, Sheep, Goat, Pig•Specimen: Plasma, Serum•Reading time : 1 hour and 45 minutes•Sensitivity: Cattle 93.8%•Specificity: Cattle 99.9%, Pig 99.9%, Sheep & goat 100%
Current available product listRapid
Species Product Cat. No. Type Packing size Description Sample
PETAnimal
Rapid CPV Ag Test Kit
Rapid CHW Ag Test Kit 2.0
Rapid CDV Ag Test Kit
Rapid CCV Ag Test Kit
Rapid CPV Ag/CCV Ag Test Kit
Rapid CIV Ag Test Kit
Rapid CDV Ag/CAV Ag Test Kit
Rapid CIRD-3 Ag Test Kit
Rapid CDV Ag/CIV Ag Test Kit
Rapid CPV/CCV/Giardia Ag Test Kit
Rapid Rabies Ag Test Kit
Rapid Rota Ag Test Kit
Rapid Giardia Ag Test kit
Rapid CPV Ab Test Kit 2.0
Rapid CDV Ab Test Kit 2.0
Rapid Canine brucella Ab Test Kit
Rapid Leishmania Ab Test Kit 2.0
Rapid E.canis Ab Test Kit
Rapid Leptospira Ab Test Kit
Rapid Canine Total IgE Test Kit
Rapid CaniV-4 Test Kit
Rapid Anaplasma Ab Test Kit
Rapid Lyme Ab Test Kit
Rapid E.canis Ab/Anaplasma Ab Test Kit
Rapid Anaplasma Ab/Lyme Ab Test Kit
RG11-01DD
RG11-02DD
RG11-03DD
RG11-04DD
RC11-05DD
RG11-07DD
RC11-08DD
RC11-09DD
RC11-10DD
RC11-12CC
RG18-01DD
RG18-03DD
RG18-04DD
RB21-51DD
RB21-52DD
RB21-03DD
RB21-04DD
RB21-05DD
RB21-07DD
RB21-10DD
RC21-20DD
RB21-21DD
RB21-22DD
RC21-23DD
RC21-24DD
Device
Device
Device
Device
Device (Dual)
Device
Device (Dual)
Device (Dual)
Device (Dual)
Device (Triple)
Device
Device
Device
Device
Device
Device
Device
Device
Device
Device
Device (Pento)
Device
Device
Device (Dual)
Device (Dual)
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 5/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
Detection of Canine Parvovirus antigen
Detection of Dirofilaria immitis antigen
Detection of Canine Distemper Virus antigen
Detection of Canine Coronavirus antigen
Detection of Canine Parvovirus & Canine Coronavirus antigen
Detection of Canine Influenza Virus antigen
Detection of Canine Distemper Virus & Canine Adenovirus antigen
Detection of Canine Distemper Virus, Canine Adenovirus & Canine Influenza Virus antigen
Detection of Canine Distemper Virus & Canine Influenza Virus antigen
Detection of Canine Parvovirus, Canine Coronavirus & Giardia lamblia antigen
Detection of Rabies Virus antigen
Detection of Rotavirus antigen in cattle, pig, and dog
Detection of Giardia lamblia antigen
Titration of Canine Parvovirus antibody
Titration of Canine Distemper Virus antibody
Detection of Brucella canins antibody
Detection of Leishmania infantum antibody
Detection of Ehrlichia canis antibody
Detection of Leptospira interrogans antibody
Detection of Total IgE
Detection of Dirofilaria immitis antigen & Anaplasma, Borrelia, Ehrlichia antibody
Detection of Anaplasma phagocytophilum and platys antibody
Detection of Borrelia burgdorferi antibody
Detection of Ehrlichia canis, Anaplasma phagocytophilum/platys antibody
Detection of Anaplasma phagocytophilum/platys, Borrelia burgdorferi antibody
Feces
Whole blood, plasma or serum
Conjunctiva, feces, saliva, urine, plasma, or serum
Feces
Feces
Conjunctiva and nasal swab
Conjunctiva and nasal swab
Conjunctiva and nasal swab
Conjunctiva and nasal swab
Feces
Brain homogenates
Feces
Feces
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Rapid FeLV Ag Test Kit
Rapid FPV Ag Test Kit
Rapid FIV Ab/FeLV Ag Test Kit
Rapid FHW Ag/Ab Test Kit
Rapid FeliCheck-3 Test Kit
Rapid FCoV Ag Test Kit
Rapid FIV Ab Test Kit
Rapid FCoV Ab Test Kit
Rapid Toxoplasma Ab Test Kit
RG12-01DD
RG12-03DD
RC12-04DD
RC12-05CD
RC22-10CD
RG12-05DD
RB22-01DD
RB22-03DD
RB22-06DD
Device
Device
Device (Dual)
Device (Dual)
Device (Triple)
Device
Device
Device
Device
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
Detection of Feline Leukemia Virus antigen
Detection of Feline Panleukopaenia Virus antigen
Detection of Feline Leukemia Virus & Feline Immunodeficiency Virus antibody
Detection of Feline Heratworm Antigen & antibody
Detection of Feline Leukemia Virus, Feline Immunodeficiency Virus & Dirofilaria immitis antibody
Detection of Feline Coronavirus antigen
Detection of Feline Immunodeficiency Virus antibody
Detection of Feline Corona Virus antibody
Detection of Toxoplasma gondii antibody
Whole blood, plasma or serum
Feces
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Feces
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
LivestockAnimal
Rapid Bovi D-4 Ag Test Kit
Rapid Bovi D-5 Ag Test Kit
Rapid Bovine Coronavirus Ag Test Kit
Rapid Cryptosporidium Ag Test Kit
Rapid Rota Ag Test Kit
Rapid Bovine Brucella Ab Test Kit
Rapid Bovine TB Ab Test Kit
Rapid FMD NSP Ab Test Kit
RC13-01DD
RC13-02DD
RG13-03DD
RG13-04DD
RG18-03DD
RB23-01DD
RB23-02DD
RB28-02DD
Device (Pento)
Device (Pento)
Device
Device
Device
Device
Device
Device
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium antigen
Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium, Giardia antigen
Detection of Bovine Coronavirus antigen
Detection of Bovine Cryptosporidium antigen
Detection of Rotavirus antigen in cattle, pig, and dog
Detection of Brucella abortus antibody in cattle
Detection of Mycobacterium bovis antibody
Detection of Foot and Mouth Ddisease Vvirus antibody field infected
Feces
Feces
Feces
Feces
Feces
Whole blood, plasma or serum
Plasma, serum
Whole blood, plasma or serum
Rapid GS. Brucella Ab Test Kit
Rapid GS. Brucella Ab Test Kit
Rapid Toxoplasma Ab Test Kit (For farm animals)
RB23-06DD
RB23-06SG
RB28-05DD
Device
Strip
Device
1Test x 10/Kit
1Test x 25/Kit
1 Test x 10/Kit
Detection of Brucella antibody in sheep and goat
Detection of Brucella antibody in sheep and goat
Detection of Toxoplasma gondii antibody
Whole blood, plasma or serum
Whole blood, plasma or serum
Whole blood, plasma or serum
Rapid MERS-CoV Ag Test Kit
Rapid Camel Brucella Ab Test Kit
RG18-05SG
RB23-09SG
Strip
Strip
1Test x 25/Kit
1Test x 25/Kit
Detection of Middle East Respiratory Syndrome Coronavirus antigen
Detection of Brucella antibody in camel
Nasal, nasosopharyngeal swab or aspirates
Whole blood, plasma or serum
Rapid PED Ag Test Kit
Rapid TGE Ag Test Kit
Rapid TGE Ag/PED Ag Test Kit
Rapid SIV Ag Test Kit
Rapid PED Ag/Rota Ag Test Kit
Rapid FMD NSP Ab Test Kit
RG14-01DD
RG14-02DD
RG14-03DD
RG14-04DD
RG14-05DD
RB28-02DD
Device
Device
Device (Dual)
Device
Device (Dual)
Device
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 10/Kit
Detection of Porcine Epidemic Diarrhea Virus antigen
Detection of Transmissible Gastroenteritis Virus antigen
Detection of TGE and PED Virus antigen
Detection of Swine Influenza Virus antigen
Detection of PED and Rotavirus antigen
Detection of Foot and Mouth Disease Virus antibody field infected
Feces
Feces
Feces
Nasal fluid or tracheal swab and lung tissue
Feces
Whole blood, plasma or serum
Rapid AIV Ag Test Kit
Rapid NDV Ag Test Kit
Rapid IBDV Ag Test Kit
Rapid H5 AIV Ag Test Kit
Rapid H7 AIV Ag Test Kit
Rapid H9 AIV Ag Test Kit
Rapid AIV Ag/NDV Ag Test Kit
Rapid IBV Ag Test Kit
Rapid AIV Ab Test Kit
RG15-01DG
RG15-03DD
RG15-04DD
RG15-06DG
RG15-07DG
RG15-08DG
RC15-09DD
RG15-13DD
RB25-01MH
Device
Device
Device
Device (3lines)
Device (3lines)
Device (3lines)
Device (Dual)
Device
Device (Multi)
1Test x 25/Kit
1Test x 10/Kit
1Test x 10/Kit
1Test x 25/Kit
1Test x 25/Kit
1Test x 25/Kit
1Test x 10/Kit
1Test x 10/Kit
10Tests x 3/Kit
Detection of Avian Influenza type A Virus antigen
Detection of Newcastle Disease Virus antigen
Detection of Infectious Bursal Disease Virus antigen
Detection of Avian Influenza type A and subtype H5 Virus antigen
Detection of Avian Influenza type A and subtype H7 Virus antigen
Detection of Avian Influenza type A and subtype H9 Virus antigen
Detection of Avian Influenza type A and Newcastle Disease Virus antigen
Detection of Infectious Bronchitis Virus antigen
Detection of Avian Influenza Virus Type A antibody
Feces or Trachea swab
Feces or Trachea swab
Bursa of Fabricious, Feces
Feces or Trachea swab
Feces or Trachea swab
Feces or Trachea swab
Feces or Trachea swab
Feces or Trachea swab
Plasma, serum
For further information, please scan this QR-code on your mobile.
" "ELISA
Species Product Cat. No. Type Packing size Description SampleBovine Brucella Ab ELISA
BTB Ab ELISA
FMD NSP Ab ELISA
TB feron ELISA
EB43-01PO
EB43-04PO
EB48-01PO
EG38-01PO
Microplate
Microplate
Microplate
Microplate
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
150 Tests/Kit
Detection of Brucella abortus antibody
Detection of Mycobacterium bovis antibody
Detection of Foot and Mouth Disease Virus antibody field infected
Detection of Gamma-interferon in bovine tuberculosis
Serum, plasma, raw milk
Serum, plasma
Serum, plasma
Plasma
CSFV Ab ELISA
PRRS Ab ELISA 4.0
PED IgA ELISA
PCV2 Ab ELISA
EB44-13PO
EB44-04PO
EB44-10PO
EB44-17PO
Microplate
Microplate
Microplate
Microplate
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
Detection of Classical Swine Fever Virus antibody
Detection of Porcine Reproductive and Respiratory Syndrome Virus antibody
Detection of IgA for Porcine Epidemic Diarrhea Virus
Detection of Porcine Circovirus antibody
Serum, plasma
Serum, plasma
Colostrum
Serum, plasma
AIV Ab ELISA
H5 AIV Ab ELISA
NDV Velo Ab ELISA
IBV Ab ELISA
NDV Ab ELISA
IBDV Ab ELISA
EB45-02PO
EB45-03PO
EB45-01PO
EB45-05PO
EB45-06PO
EB45-07PO
Microplate
Microplate
Microplate
Microplate
Microplate
Microplate
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
480 Wells/Kit
Detection of AIV type A antibody
Detection of AIV subtype H5 antibody
Detection of velogenic Newcastle Disease Virus antibody
Detection of Infectious Bronchitis Virus antibody
Detection of Newcastle Disease Virus antibody
Detection of Infectious Bursal Disease Virus antibody
Serum
Serum
Serum
Serum
Serum
Serum
Conventional PCRSpecies Product Cat. No. Type Packing size Description Sample
LivestockAnimal
AIV Detection Test Kit
H5 AIV Detection Test Kit
H7 AIV Detection Test Kit
H9 AIV Detection Test Kit
Lento NDV Detection Test Kit
Velo NDV Detection Test Kit
IBDV Detection Test Kit
PD55-01ON
PD55-02ON
PD55-03ON
PD55-04ON
PD55-07ON
PD55-08ON
PD55-11ON
Conventional PCR
Conventional PCR
Conventional PCR
Conventional PCR
Conventional PCR
Conventional PCR
Conventional PCR
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
Detection of AIV type A
Detection of AIV subtype H5
Detection of AIV subtype H7
Detection of AIV subtype H9
Detection of low pathogenci NDV (Lentogenic)
Detection of high pathogenic NDV (Velogenic)
Detection of Infectious Bursal Disease Virus
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Real-Time PCRSpecies Product Cat. No. Type Packing size Description Sample
LivestockAnimal
AIV A Real-Time Detection Test Kit
H5 AIV Real-Time Detection Test Kit
H7 AIV Real-Time Detection Test Kit
H9 AIV Real-Time Detection Test Kit
REV Real-TIme Detection Test Kit
MDV Real-TIme Detection Test Kit
Lento NDV Real-Time Detection Test Kit
Velo NDV Real-Time Detection Test Kit
IBV Real-Time Detection Test Kit
TRT Real-Time Detection Test Kit
IBDV Real-Time Detection Test Kit
REO Real-Time Detection Test Kit
MYCO G/S Real-Time Detection Test Kit (2 Wells type)
ALV Real-Time Detection Test Kit
AIV H5/N1 Duplex Real-Time Detection Test Kit
PD65-01ON
PD65-02ON
PD65-03ON
PD65-04ON
PD65-05ON
PD65-06ON
PD65-07ON
PD65-08ON
PD65-09ON
PD65-10ON
PD65-11ON
PD65-12ON
PD65-14ON
PD65-15ON
PD65-51ON
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
Detection of AIV type A
Detection of AIV subtype H5
Detection of AIV subtype H7
Detection of AIV subtype H9
Detection of Reticuloendotheliosis Virus
Detection of Marek's disease Virus
Detection of low pathogenci NDV (Lentogenic)
Detection of high pathogenic NDV (Velogenic)
Detection of Infectious Bronnchitis Virus
Detection of Infectious Turkey Rhinotracheitis
Detection of Infectious Bursal Disease Virus
Detection of Avian Reovirus
Detection of Avian Mycoplasma gallisepticum, and M. iowae, M. meleagridis , M. synoviae
Detection of Avian Leukosis Virus
Detection of AIV H5 and N1
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
SIV Real-Time Detection Test Kit (2 Wells type)
PCV-2 Real-Time Detection Kit
PRRSV Real-Time Detection Kit (2 Wells type)
CSFV Real-Time Detection Kit
PD64-02
PD64-03
PD64-04
PD64-05
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
Detection of Swine Influenza Virus
Detection of Procine Circovirus
Detection of Procine Reproductive and Respiratory Syndrome Virus
Detection of Classical Swine Fever Virus
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
BTV Real-Time Detection Kit
IBR Real-Time Detection Kit
BVDV Real-Time Detection Kit
FMD Real-Time Detection Kit
PD63-03
PD63-04
PD63-05
PD68-01
Real-Time PCR
Real-Time PCR
Real-Time PCR
Real-Time PCR
96Tests/Kit
96Tests/Kit
96Tests/Kit
96Tests/Kit
Detection of Bluetongue Virus
Detection of Infectious Bovine Rhinotracheitis Virus
Detection of Bovine Viral Diarrhea Virus
Detection of Foot and Mouth Disease Virus
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
Sercetion, organ, blood, feces, fluid etc.
│Ver.4.1Product Catalog
CIA 15-04E1
Livestock