Livestock Product Catalog - lab- · PDF file7 AIV Ab Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected

│Ver.4.1Product Catalog

CIA 15-04E1

Livestock

03040506

07080911

03 Anigen, Inc. established

11 Acquired animal medicine import license

12 Acquired animal medicine manufacturing industry permission

09 Acquired ISO 9001:2000 TUV certi�cation

08 Moved to the new Young-tong factory

01 Purchased the site for the new Dongtan factory

04 Canine Distemper virus antibody diagnostic kit patent acquisition

04 Canine Parvovirus antibody diagnostic kit patent acquisition

06 Venture business certi�cation (No. 061627021-01133)

07 Technical innovation medium and small enterprises certi�cation (No.6065-1216)

07 Gyeonggi-do excellent medium and small enterprises selection

09 H5 and general pathogenic avian in�uenza virus antigen diagnostic

11 Name change of Anigen, Inc. to Animal Genetics, Inc.

12 Winner of the Animal medicine manufacturer selfregulation checking system Prize

02 World’s �rst In�uenza H3N2 type con�rmation assay development

07 Novel canine in�uenza viruses and vaccine patent acquisition


01 Anigen AIV Ab ELISA knowledge economics department new product certi�cation (NEP) acquisition

02 Name change of Animal genetics, Inc. to BioNote, Inc.

03 Moved to new Dongtan factory

05 Food & Drug Administration medicine manufacturing industry permission acquisition

12 ISO 2003:13485 TUV certi�cation acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed

11. 01 Acquisition by Alere

12. 02 Enlarged production facility and HQ

12. 04 Applied for International Patent for A Novel Canine In�uenza virus and Vaccine Therefore (PCT)

INTRODUCTIONBioNote, Inc. was established in the beginning of 2003, and is considered a pioneer of in-vitro Diagnostics for veterinary needs. BioNote delivers innovative and creative solutions for our customers improving their vet healthcare. BioNote has it's own automated facility to manufacture a wide range of high quality products developed by a highly competent R&D center. BioNote has manufactured diagnostic products in accordance with ISO 9001:ISO13485 certification, and has expanded overseas sales network in over 80 countries and is still continuing to grow.

If you want to know more about BIONOTE, please scan this QR-code on your mobile

03040506

07080911

03 Anigen, Inc. established

11 Acquired animal medicine import license

12 Acquired animal medicine manufacturing industry permission

09 Acquired ISO 9001:2000 TUV certi�cation

08 Moved to the new Young-tong factory

01 Purchased the site for the new Dongtan factory

04 Canine Distemper virus antibody diagnostic kit patent acquisition

04 Canine Parvovirus antibody diagnostic kit patent acquisition

06 Venture business certi�cation (No. 061627021-01133)

07 Technical innovation medium and small enterprises certi�cation (No.6065-1216)

07 Gyeonggi-do excellent medium and small enterprises selection

09 H5 and general pathogenic avian in�uenza virus antigen diagnostic

11 Name change of Anigen, Inc. to Animal Genetics, Inc.


02 World’s �rst In�uenza H3N2 type con�rmation assay development

07 Novel canine in�uenza viruses and vaccine patent acquisition


01 Anigen AIV Ab ELISA knowledge economics department new product certi�cation (NEP) acquisition

02 Name change of Animal genetics, Inc. to BioNote, Inc.

03 Moved to new Dongtan factory

05 Food & Drug Administration medicine manufacturing industry permission acquisition

12 ISO 2003:13485 TUV certi�cation acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed

11. 01 Acquisition by Alere

12. 02 Enlarged production facility and HQ

12. 04 Applied for International Patent for A Novel Canine In�uenza virus and Vaccine Therefore (PCT)

HISTORY

Purchased the site for the new Dongtan factoryCanine Distemper virus antibody diagnostic kit patent acquisitionCanine Parvovirus antibody diagnostic kit patent acquisition Venture business certification (No. 061627021-01133) Technical innovation medium and small enterprises certification (No.6065-1216)

World’s first Influenza H3N2 type confirmation assay developmentNovel canine influenza viruses and vaccine patent acquisitionWinner of the Animal medicine manufacturer selfregulation checking system Prize

Acquisition by Alere

Built the 2nd building for Manufacturing, Warehouse, R&D lab.Registered International Patent (PCT) for “A Novel Canine Influenza virus and Vaccine”

AniGen TB-feron ELISA Kit was assigned as an official diagnosis method for bovine tuberculosis eradication program in Korea

Spun off as an independent company from Alere, Inc. (USA)Expanded Manufacturing facility

Anigen, Inc. establishedAcquired animal medicine import licenseAcquired animal medicine manufacturing industry permission

Acquired ISO 9001:2000 TUV certification

Moved to the new Young-tong factory

Gyeonggi-do excellent medium and small enterprises selectionH5 and general pathogenic avian influenza virus antigen diagnosticName change of Anigen, Inc. to Animal Genetics, Inc.Winner of the Animal medicine manufacturer selfregulation checking system Prize

Anigen AIV Ab ELISA knowledge economics department new product certification (NEP) acquisitionName change of Animal genetics, Inc. to BioNote, Inc.Moved to new Dongtan factoryFo o d & D ru g Ad m i n i s t rat i o n m e d i c i n e manufacturing industry permission acquisitionISO 2003:13485 TUV certification acquisition Anigen Rapid Canine Heartworm Ag Test Kit USDA licensed

2003. 032003. 112003. 12

2004. 09

2005. 08

2007. 07

2007.09

2007.11

2007. 12

2009. 01

2009. 02

2009. 032009. 05

2009. 12

2006. 012006. 04

2006. 04

2006. 06

2006. 07

2008. 02

2008. 07

2008. 12

2011. 01

2012. 02

2012. 04

2013. 12

2014. 11

2014. 12

2003

2006

2008

2007

2009

2011~2014

II RapidITest

05IAIVIAg06IH5IAIVIAg07IAIVIAb08INDVIAg09IIBVIAg

10IIBDVIAg

II ELISA

11IAIVIAbIELISA12IH5IAIVIAbIELISAI13INDVIAbIELISAI14IIBVIAbIELISA15IIBDVIAbIELISA

II PCRIKitsIforIAvianIdisease

AvianIproduct >>

Poultry Sampling Video Clip

Rapid Test Procedure Video Clip

* Please scan QR-codes for more information of poultry sampling and rapid test procedure

5

AIV Ag Influenza viruses that infect birds are called "Avian Influenza virus (AIV)" with only influenza type A viruses infecting birds. Influenza type A viruses are divided into subtypes based on two proteins, hemagglutinin (HA) and neuraminidase (NA), on the surface of the virus. AIV can infect chickens, turkeys, pheasants, quail, ducks, geese, and guinea fowl, as well as wide variety of birds. AIV is transmitted primarily through direct contact from infected birds to healthy birds, and through indirect contact with contaminated equipment and materials. The virus is excreted through infected birds' feces and secretions from their noses, mouths, and eyes.

Ordering Information

Test Procedures

•Field monitoring of Avian Influenza virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases

Indications

Special Features•Detection of all AIV type A•No cross-reaction with other avian viruses•Applicable to various species•World's first commercialized rapid test kit for AIV

C

C

T

2 1

S

AIV

Ag

Take the supernatant with disposable dropper provided.

Add 4 drops into the sample hole with disposable dropper.

4 drops

1

2

3 4

Collect swab sample from trachea, kidney, spleen or cloaca(feces.)

Eye

Bronchial Tubes

Cloaca

Small Intestine

Mesentery

Large Intesline

Oviduct

KidneyOvaryCeca

Lungs

Duodenal Loop

Pancreas

Heart

EsophagusTrachea

Crop

proventriculusGall Bladder

SpleenLiver

Gizzard

OropharynxSuperior Larynx

Eye

Cloaca

Small Intestine

Oviduct

KidneyOvary

Lungs

Pancreas

Heart

Trachea

Crop

Gall Bladder

SpleenLiver

Gizzard

Insert the swab into the sample tube containing assay diluent, and mix the swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.

20 min. C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Avian Influenza type A virus antigen

•Validated from OIE reference laboratories•Specimen: Trachea, kidney, spleen or cloaca(feces) •Sensitivity: 100% by farm (n=19), 77.3% by feces (n=150)•Specificity: 100% vs. HA, PCR (n=1,402)

Validated from *VLA, Veterinary Laboratory Agency, United Kingdom *FLI, Friedrich Loeffler Institute FLI, Germany *CSIRO, Australian Animal Health Laboratory *FGI «ARRIAH», Federal Center For Animal Health, Russia

Cat. No. Description Type Packing sizeRG15-01DG

Rapid AIV AgDevice 1 Test x 25/Kit

RG15-01MH Multi device 10 Tests x 3/Kit

Avi

an

6

H5 AIV Ag Avian influenza A viruses can be classified as low and high pathogenic form based on the severity of the illness they cause. Avian influenza A subtype H5 and subtype H7 viruses can be considered as "high pathogenic" strains on the basis of genetic features of the virus and the severity of the illness. Avian influenza A subtype H5 potentially has nine different subtypes (H5N1 ~ H5N9) and these subtypes can be highly pathogenic (HPAI) or low pathogenic (LPAI). The Influenza type A subtype H5 infection has been reported among humans and sometimes causes severe illness and death.

Ordering InformationCat. No. Description Type Packing size

RG15-05DGRapid H5 AIV Ag

Device 1 Test x 25/KitRG15-05MH Multi device 10 Tests x 3/Kit

Test Procedures

•Field monitoring of Avian Influenza virus subtype H5•Tentative diagnosis for swift control in outbreak suspected situation•Rule out low pathogenic AIV strain

Avian Influenza type A subtype 5 virus antigen

Indications

Special Features•H5 AIV detection only •No cross-reaction with other avian viruses•Applicable to various species•World's first commercialized rapid test kit for AIV H5•Validated from OIE reference laboratories

C

C

T

2 1

S

H5 A

IV A

g


Add 4 drops into the sample holewith disposable dropper.

4 drops

2

3 4

Collect swab sample from trachea, kidney, spleen or cloaca(feces.)

Eye

Bronchial Tubes

Cloaca

Small Intestine

Mesentery

Large Intesline

Oviduct

KidneyOvaryCeca

Lungs

Duodenal Loop

Pancreas

Heart

EsophagusTrachea

Crop

proventriculusGall Bladder

SpleenLiver

Gizzard

OropharynxSuperior Larynx

Eye

Cloaca

Small Intestine

Oviduct

KidneyOvary

Lungs

Pancreas

Heart

Trachea

Crop

Gall Bladder

SpleenLiver

Gizzard

1


20 min.

•Specimen: Trachea, kidney, spleen or cloaca(feces) •Sensitivity: 100% by farm (n=13), 76.6% by feces

(n=115) vs. Virus isolation•Specificity: 100% vs. Virus isolation, PCR (n=1,402)

Validated from *FGI «ARRIAH», Federal Center For Animal Health, Russia *Giessen University, Gemany *IZS Umbria e Marche, Peruzia, Italy

H5

AIV

Ag

InterpretationC 2 1

Negative

C 2 1H5 AIV Positive

AIV Positive

C 2 1

Invalid

7

AIV Ab Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen Rapid AIV Ab Test Kit has been developed for screening these latent AIV carriers by detecting AIV antibodies.


RB25-01MH Rapid AIV Ab Multi device 10Tests x 3/Kit

Test Procedures

•Avian influenza virus antibody detection•Field monitoring of Avian Influenza virus carrier•Antibody detection for migratory bird surveillance

Indications

Special Features•No cross-reaction with other avian virus positive sera•Applicable to various species•The world’s first rapid test kit for antibody to AIV

C TC 1 2

AIV Ab

Serum 1 1 drop of serum2 3 drops of assay diluent3

20 min.

C TC 1 2

AIV Ab

"Test result with 2 lines is a negative result and 1 line is a positive result."

Avian Influenza type A antibody

Anigen Rapid Avian Influenza Virus Antibody Test Kit has been validated against following antisera

H1N1 H2N3 H3N2 H3N8 H4N8 H5N1 H5N2H5N3 H6N2 H7N1 H7N3 H7N7 H8N4 H9N2H9N7 H10N1 H11N6 H12N5 H13N6 H14N2 H15N6

Performance characteristics•The detection limit of this kit is approximately HI titer 1:16•Serotype validation

•Specimen: Serum•Sensitivity: 96% vs. HI•Specificity: 96.5% vs. HI

Interpretation

Avi

an

8

NDV Ag Newcastle Disease (ND) is characterized by sneezing, coughing, and neurologic sign. Mortality rate is especially very high in the young age group (about 90%). Affected layer has poor egg quality and reduced egg production in initial phase but usually return to normal levels within four to eight weeks. ND can cause sudden death even in vaccinated poultry and spread rapidly to nearby flocks. Diagnosis is usually made by virus isolation, serology and clinical signs. It has been reported that hemagglutination inhibition (HI) test which is used widely in ND virus serology has cross reactions with other paramyxoviruses. The Anigen Rapid NDV Ag test kit is highly specific to NDV.


RG15-03DD Rapid NDV Ag Device 1 Test x 10/kit

Test Procedures

•Field monitoring of Newcastle Disease virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases

Indications

Special Features•Detection of all NDV•No cross-reaction with other avian viruses •World's first commercialized rapid test kit for detection of NDV

Eye

Cloaca

Small Intestine

Oviduct

KidneyOvary

Lungs

Pancreas

Heart

Trachea

Crop

Gall Bladder

SpleenLiver

Gizzard

C

C

T

2 1

S

ND

V A

g



1

2

3 4

Collect swab sample from oropharynx, kidney or spleen.

4 drops

10 min.


C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Newcastle Disease virus antigen

•Specimens: Trachea, kidney or spleen•Sensitivity: 94.7% vs. RT-PCR•Specificity: 96.4% vs. RT-PCR

H5

AIV

Ag

9

IBV Ag Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all ages of chickens. The mortality rate is depending on the age and immune status of the birds when infected, and the presence of secondary invading organisms such as E. coli. It is highly contagious disease that can be transmitted through an entire flock within one or two days, through aerosol transmission (sneezing), contaminated organic material, drinking water and equipment. The target organs of the virus are trachea and kidney for the respiratory strain and nephrogenic strain respectively. It is financially important disease in poultry industry because affected layers have decreased egg production and poor egg quality.


RG15-13DD Rapid IBV Ag Device 1Test x 10/Kit

Test Procedures

•Field monitoring of Infectious Bronchitis virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases

Indications

Special Features•Detection of all IBV•No cross-reaction with other avian viruses •World's first commercialized rapid test kit for IBV

C

C

T

2 1

S

IBV

Ag

Insert the swab into the sample tube containing assay diluent and mix the swab until the sample has been dissolved into the assay diluent and squeeze the swab against the well of tube and then discard it.



1

2

3 4

Collect swab sample from trachea, kidney or cloaca (feces).

4 drops

Eye

Cloaca

Small Intestine

Oviduct

KidneyOvary

Lungs

Pancreas

Heart

Trachea

Crop

Gall Bladder

SpleenLiver

Gizzard

10 min.C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Infectious bronchitis virus antigen

•Specimens: Trachea, kidney or cloaca(feces) •Sensitivity: 94.1% vs. RT-PCR•Specificity: 95.2% vs. RT-PCR

H5

AIV

Ag

Avi

an

10

IBDV Ag Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually affecting young chickens 3 to 6 weeks old, and transmitted by contaminated feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an important organ for young chickens as an immune development. IBDV serotype 1 causes clinical disease in chickens younger than 10 weeks, with older chickens usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is sometimes found in chickens and ducks. In practice, a diagnosis can be indicated by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and the presence of distinctive lesions in the Bursa of Fabricious and accompanying blood spots in the musculature of the breast and thigh of affected chickens


RG15-04DD Rapid IBDV Ag Device 1 Test x 10/Kit

Test Procedures

•Field monitoring of Infectious Bursal Disease virus•Tentative diagnosis for swift control in outbreak suspected situation•Differential diagnosis of other avian major diseases

Indications

Special Features•Detection of all IBDV•No cross-reaction with other avian viruses •Specimen: Bursa of Fabricious, Cloaca

C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Infectious Bursal Disease virus antigen

•World's first commercialized rapid test kit for IBDV•Sensitivity: 99.9% vs. RT-PCR•Specificity: 96.6% vs. RT-PCR

C

C

T

2 1

S

IBD

V A

g

Insert the swab into the sample tube containing assay diluent and mix the swab until the sample has been dissolved into the assay diluent.



4 drops

2

3 4Collect swab sample from Bursa of Fabricious or cloaca (feces)

Eye

Cloaca

Small Intestine

Oviduct

KidneyOvary

Lungs

Pancreas

Heart

Trachea

Crop

Gall Bladder

SpleenLiver

Gizzard

1

10 min.

H5

AIV

Ag

11

AIV Ab ELISA


EB45-02PO AIV Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Avian influenza virus antibody detection•Monitoring of Avian Influenza virus carrier•Antibody detection for migratory bird surveillance

Indications

Special Features•No cross-reaction with other avian virus positive sera•Applicable to various species•Specimen: Serum, plasma or egg yolk•No sample dilution is required

1. Prepare AIV NP antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of anti AIV antibody-HRP to each well4. Incubate plate for 30 minutes at 37℃

5. Wash plate 6 times6. Add 100㎕ of substrate (Ready to use) and incubate the wells

for 10 minutes at room temperature7. Add 100㎕ of stopping solution8. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm9. PI value=[1-(OD sample/mean OD negative)] x 100

Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen AIV Ab ELISA has been developed for screening these latent AIV carriers by detecting AIV antibodies.

3 days

0 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

1.2

1

0.8

0.6

0.4

0.2

0

AniGen AIV ELISA

HI test

Starting point of Ab detection

Evaluation of AIV Antibody detection in egg yolk

*AniGen AIV Ab ELISA kit showed that it can detect the antibody in egg yolk approximately 3~5 days earlier than HI test.

Avian Influenza type A virus antibody

•Reading time: 45 minutes•Sensitivity: Chicken 98.2%, Duck 97.5%, Turkey 97.1%•Specificity: Chicken 97.3%, Duck 93.8%, Turkey 100%

Validated from *IZS delle Venezie, Legnaro (Padova), Italy *FGI «ARRIAH», Federal Center For Animal Health, Russia *University of Hokkaido, Graduated School of Veterinary Medicine

Avi

an

12

H5 AIV Ab ELISA


EB45-03PO H5 AIV Ab ELISA Microplate 480 Wells/ Kit

Quick Procedure

•Antibody titer check after vaccination •H5 AIV carrier monitoring in non-vaccinated group •H5 AIV antibody detection for prevention program

Indications

Special Features•No cross-reaction with other AIV subtype antibodies •Species: Chicken, duck, quail•Specimen: Serum, Plasma•No sample dilution is required

1. Prepare AIV H5 antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of Mab-HRP (1:100 dilution in the conjugate diluent)

to all wells4. Incubate plate for 90 minutes at 37℃

5. Wash plate 6 times6. Add 100㎕ of substrate solution and incubate for 15 minutes

at room temperature7. Add 100㎕ of stop solution8. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm9. PI value= [1-(OD sample/mean OD negative)] x 100

Wild birds and some poultry infected by HPAI subtype H5 show no clinical signs, and their virus shedding amount is too small to be detected by an antigen capture immunoassay. The AniGen H5 AIV Ab ELISA has been developed for screening these latent H5 AIV carriers by detecting AIV subtype H5 antibodies.

Serotype validation - AniGen H5 AIV Ab ELISA detects only H5 serotype- High sensitivity and specificity compared with HI test serotype validation

H1N1 H3N2 H2N9 H5N2 H5N1 H5N3 H7N7 H9N2

120

100

80

60

40

20

0

PI 50

Serotype validation

PI v

alue

Avian Influenza type A subtype H5 virus antibody

• Reading time: 1 hour and 45 minutes•Sensitivity: Chicken 96.9% (vs. HI)•Specificity: Chicken 100 % (vs. HI)

13

NDV Ab ELISA Newcastle disease is a contagious bird disease affecting many domestic and wild avian species. NDV strains can be categorized as velogenic (highly virulent), mesogenic (intermediate virulence) or lentogenic (non virulent). Velogenic strain is very contagious and produces severe nervous and respiratory signs and catastrophic (90% of mortality or more). Mesogenic strain causes cough, affect egg quality and production and results in about 10% of mortality. Lentogenic strains produce mild signs with negligible mortality. In acute cases, the death is very sudden, and, in the beginning of the outbreak, the remaining birds do not seem to be sick. In flock with good immunity, the signs (respiratory or gastrointestinal) are mild and progressive, and are followed after 7 days by nervous symptoms, especially twisted heads.


EB45-06PO NDV Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Routine monitoring of NDV•NDV diagnosis of non-vaccinated group•Antibody titer check after vaccination

Indications

Special Features•Optimal screening method for NDV•Reading time: 75 minutes•Specimen: Serum

1. Prepare NDV antigen coated test plate2. Dilute test sample with sample diluent to 1:500

Do not dilute controls3. Add the 100㎕ of control sera and diluted test samples to

each well4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate and incubate for 30

minutes at room temperature7. Wash the wells 5 times8. Add 100㎕ of substrate solution to each well9. Incubate the wells for 15 minutes at room temperature10. Add 100㎕ of stopping solution to each well11. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm12. S/P ratio= [OD sample-mean OD negative] / [mean OD

positive- mean OD negative] x 100

Antibody development after vaccination and challengePositive rate was 100% after 3 weeks of vaccination in both ELISA (Shade zone)AniGen ELISA S/P ratio was generally higher than “I” commercial ELISA

Anigen

IDEXX

S/P

ratio

day0 day7 day14 day21 day28 day35 day42

Vaccination (IB,/ND) NDV challenge

8

7

6

5

4

3

2

1

0

-1

Newcastle Disease virus antibody

•Sensitivity: 98.4% vs. “I” commercial ELISA•Specificity: 97.7% vs. “I” commercial ELISA

Avi

an

14

IBV Ab ELISA Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all ages of chickens. The morbidity rate is extremely high and the mortality rate is dependent on the age of the chickens when infected, and the presence of secondary invading organisms such as E. coli. It is highly contagious disease that can be transmitted through an entire flock within one or two days, through aerosol transmission (sneezing), contaminated organic material, drinking water and equipment. The target organs of the virus are trachea and kidney for the respiratory strain and nephrogenic strain respectively. It is financially important disease in poultry industry because affected layers have decreased egg production and poor egg quality.


EB45-05PO IBV Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Routine monitoring of IBV• IBV diagnosis of non-vaccinated group•Antibody titer check after vaccination

Indications

Special Features•Optimal screening method for IBV•Reading time: 75 minutes•Specimen: Serum

1. Prepare IBV antigen coated test plate2. Dilute test sample with sample diluent to 1:500

Do not dilute controls3. Add the 100㎕ of control sera and diluted test samples to

each well4. Incubate the wells at room temperature (18~25℃) for 30

minutes5. Wash the well at 5 times6. Add 100㎕ of enzyme conjugate to each well7. Incubate the wells at 18~25℃(Room Temperature) for 30

minutes and wash the wells 5 times8. Add 100㎕ of substrate solution to each well and incubate

the wells for 15±1 minutes at room temperature (18~25℃)9. Add 100㎕ of stopping solution10. Read the absorbance of the wells with a spectrophotometer

at 450nm with reference wavelength at 620nm11. S/P value=[OD samples - OD of negative control]/[OD

positive control – OD of negative control]

Positive rate after vaccinationAniGen IBV ELISA shows higher positive rate and S/P ratio than “B” ELISAMaternal antibody interference effect is shorter in Anigen than “B” ELISA (Shade zone)

0

20

40

60

80

100

WPI

Posi

tive

rate

WPI

S/P

ratio

3.5

3

2.5

2

1.5

1

0.5

0

Anigen IBV Ab ELISA

“B” IBV Ab ELISA

1 3 5 7 10 14 18 22

1 3 5 7 10 14 18 22

Infectious Bronchitis virus antibody


15

IBDV Ab ELISA Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually affecting young chickens 3 to 6 weeks old, and transmitted by contaminated feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an important organ for young chickens as an immune development. IBDV serotype 1 causes clinical disease in chickens younger than 10 weeks, with older chickens usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is sometimes found in chickens and ducks. In practice, a diagnosis can be indicated by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and the presence of distinctive lesions in the Bursa of Fabricious and accompanying blood spots in the musculature of the breast and thigh of affected chickens


EB45-07PO IBDV Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Routine monitoring of IBDV• IBV diagnosis of non-vaccinated group•Antibody titer check after vaccination

Indications

Special Features•Optimal screening method for IBDV•Reading time: 75 minutes•Specimen: Serum

1. Prepare IBV antigen coated test plate2. Dilute test sample with sample diluent to 1:500

Do not dilute controls3. Add the 100㎕ of control sera and diluetd test samples to

each well4. Incubate the wells at room temperature (18~25℃) for 30

minutes5. Wash the wells 5 times6. Add 100㎕ of enzyme conjugate to each well7. Incubate the wells at 18~25℃(Room Temperature) for 30

minutes and wash the wells 5 times8. Add 100㎕ of substrate solution to each well and incubate

the wells for 15±1 minutes at room temperature (18~25℃)9. Add 100㎕ of stopping solution10. Read the absorbance of the wells with a spectrophotometer

at 450nm with reference wavelength at 620nm11. S/P ratio=[OD450 samples - OD450 NCx] / [OD450 PCx -

OD450 NCx]

Infectious Bursal Disease virus antibody


Antibody detection after vacciantion- AniGen IBDV Ab ELISA detects IBDV Ab 2weeks after vaccination

0.000

0.500

1.000

1.500

2.000

2.500

11(DPI-0)

17(DPI-0)

24(DPI-4)

27(DPI-7)

31(DPI-11)

34(DPI-14)

38(DPI-18)

41(DPI-21)

S/P

Age

BioNote

“I” commercial ELISA

0.2

Avi

an

16

PCR Kits for Avian disease

List of PCR Kits for Avian disease

•Confirm poultry diseases by antigen detection •Differential diagnosis of difficult case shows similar signs•Use to clarify signs of infection in vaccinated group showing uncertain antibody titer

Indications

Special Features•Ultimate diagnostic system for poultry infectious disease •Optimal and unique primers and probes system guarantees highly specific result•BioNote general PCR Detection Kits contain all components for the detection of agents• Includes positive and negative control•More convenient method than virus culture•One step PCR using Taqman probe (Real-Time)•Enables quantitative and qualitative analysis (Real-Time)•Higher detection limit than general PCR : 100~1,000 times more sensitive (Real-Time)

Product Diagnosis Cat. No.

General

AIV Detection Kit AIV type A (Matrix gene) PD55-01ON

AIV H5 Detection Kit Subtype H5 PD55-02ON

Velo NDV Detection Kit High pathogenic NDV virus (Velogenic) PD55-08ON

IBDV Detection Kit Infectious Bursal Disease virus PD55-11ON

Real-time

AIV A Real-Time Detection Kit AIV H5/N1 Duplex Detection Kit PD65-01ON

Velo NDV Real-Time Detection Kit High pathogenic NDV virus (Velogenic) PD65-08ON

IBV Real-Time Detection Kit Infectious Bronchitis Virus PD65-09ON

IBDV Real-Time Detection Kit Infectious Bursal Disease Virus PD65-11ON

MYCO G/S Real-Time Detection Kit Avian Mycoplasma gallisepticum, and M. iowae, M. meleagridis , M. synoviae PD65-14ON

II RapidITest

18IPEDIAg19ITGEIAg20IRotaIAg

II ELISA

21ICSFVIAbIELISA22IPRRSIAbIELISAI4.024IPEDIIgAIELISA25IPCV-2IAbIELSIA

II PCRIKitsIforISwineIdisease

SwineIproduct >>

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18

PED Ag Porcine epidemic diarrhea virus (PEDV) is a member of the coronavirus group which causes watery diarrhea, dehydration and high mortality in suckling pigs. Porcine Transmissible Gastroenteritis Virus (TGEV) is serologically unrelated with PEDV, but clinically these two virus infections are difficult to differentiate. The PEDV is transmitted by feces from infected pigs after oral ingestion. Current available laboratory diagnosis for PED is a cryostat section - direct immunofluorescence assay of the small intestine or colon. ELISA and RT-PCR assays to detect viral antigens in feces or intestinal contents are useful, but require specialized instruments and laboratory personnel. The Anigen Rapid PED Ag test kit has been developed to provide fast and reliable test results of PED antigen in field conditions.


RG14-01DD Rapid PED Ag Device 1 Test x 10/KitRG14-05DD Rapid PED/Rota Ag Dual Device 1 Test x 10/Kit

Test Procedures

•Differential diagnosis of swine diarrheal disease •PEDV field monitoring•Tentative diagnosis for swift control in outbreak suspected situation

Indications

Specifications•No cross reaction with other etiologic agents•World's first & sole PED virus antigen rapid test kit•Specimen : Diarrheal feces•Sensitivity: 92% vs. RT-PCR•Specificity: 98% vs. RT-PCR

C

C

T

2 1

S

PE

D A

g

or

Collect sample from diarrheal feces using the swab.1

3 4

2

Wait 30 seconds for sedimentation and take the supernatant with disposable dropper provided.


4 drops

Insert the swab into the sample tube containing assay diluent, and mixthe swab until the sample has been dissolved into the assay diluent.Squeeze the swab against the well of tube and then discard it.

5~10min.C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Porcine Epidemic Diarrhea virus antigen

H5

AIV

Ag

Not enough Good Too much

[Proper sample amount collected by a swab]

19

TGE Ag Transmissible gastroenteritis (TGE) is a viral disease of the small intestine that causes vomiting and diarrhea in pigs of all ages. The infection spreads rapidly by aerosol or contact exposure. Severe epidemics are more common during winter due to survival of the virus in colder temperatures. Depending on the level of immunity and exposure, diarrhea may be mild in some litters but severe in others. Because TGE virus is easily spread during an epidemic by persons, animals, and other means, fast diagnosis and special care should be taken to prevent spread to unexposed groups of pigs and to neighboring herds. Current clinical signs in the epidemic form of TGE usually justify a presumptive diagnosis. In the mild endemic form, laboratory confirmation is required. The Anigen Rapid TGE test kit has been developed to provide fast and reliable test results of TGE antigen in field conditions.


RG14-02DD Rapid TGE Ag Device 1 Test x 10/KitRG14-03DD Rapid TGE/PED Ag Dual Device 1 Test x 10/Kit

Test Procedures

•Differential diagnosis of swine diarrheal disease •TGE field monitoring•Tentative diagnosis for swift control in outbreak suspected situation

Indications

Special Features•No cross reaction with other etiologic agents•World's first & sole TGE virus antigen rapid test kit•Specimen : Diarrheal feces•Sensitivity: 92.1% vs. RT-PCR•Specificity: 95.2% vs. RT-PCR

C

C

T

2 1

S

TGE

Ag

or

1

3 4

2



4 drops


Collect sample from diarrheal feces using the swab.

5~10min. C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Transmissible Gastroenteritis virus antigen

H5

AIV

Ag



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Rota Ag Rotavirus is the most common viral causative agent of diarrhea in pigs, cattle and dogs. Group A and B rotavirus are involved with group A being most prevalent and clinically important, containing several serotypes of differing virulence. All ages are susceptible. If neonatal pigs and cattle do not receive protective levels of maternal antibody, they are likely to develop profuse watery diarrhea in 12-48 hr. More commonly, the infection is endemic in a herd. Usually confirmatory diagnosis is based on histologic demonstration of villous atrophy in the jejunum or electron microscopy demonstration of virion in the intestinal contents. These methods take a long time and require high priced specialized equipment and expertise. The Anigen Rapid Rota Ag test kit has been developed to provide fast and reliable test results of Rota antigen in field conditions.


RG18-03DD Rapid Rota Ag Device 1 Test x 10/KitRG14-05DD Rapid PED/Rota Ag Dual Device 1 Test x 10/Kit

Test Procedures

•Differential diagnosis of bovine and swine diarrheal disease •Rotavirus field monitoring•Tentative diagnosis for swift control in outbreak suspected situation

Indications

Specifications•No cross reaction with other etiologic agents•World's first commercialized rapid test kit for detection of

Rotavirus antigen•Specimen : Diarrheal feces•Sensitivity: 92% vs. RT-PCR•Specificity: 99% vs. RT-PCR

C

C

T

2 1

S

Rot

a A

g

or

1

3 4

2



4 drops


Collect sample from diarrheal feces using the swab.

5~10min.C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Rotavirus antigen

H5

AIV

Ag



21

CSFV Ab ELISA Classical Swine Fever Virus (CSFV), previously called hog cholera virus, is a Pestivirus in the family Flaviviridae. The CSFV is closely related to the ruminant pestiviruses which cause Bovine Viral Diarrhea (BVDV) and Border Disease (BDV). The virulence of CSFV strains varies widely which is leading to a wide range of clinical signs. Highly virulent strains result in acute and severe clinical signs including neurological signs and hemorrhages and high mortality rate. Less virulent strains can give rise to sub-acute or chronic infections that may escape detection, while still causing abortions and stillbirth. Herds in high risk areas are usually serologically tested on a thorough statistical basis.


EB44-13PO CSFV Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Routine monitoring of CSFV•Diagnosis of CSFV by antibody titration •Titer check after vaccination

Indications

Special Features•No cross reaction with other swine disease positive sera•Blocking ELISA which is OIE reference method for CSFV •Specimen: Plasma, Serum•Reading time: 1 hour and 45 minutes•Sensitivity: 99.3% vs. Commercial ELISA•Specificity: 99.7 % vs. Commercial ELISA

1. Prepare CSFV antigen coated test plate2. Add 50㎕ of the sample diluent into each well of the plate 3. Add 50㎕ of controls and samples to wells4. Incubate plate for 60 minutes at 37℃

5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate(ready to use) into each well 7. Incubate the wells at 37℃ for 30 minutes 8. Wash plate 5 times 9. Add 100㎕ of substrate(ready to use) to each well and incubate for 15 minutes at room temperature10. Add 100㎕ of stopping solution 11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm12. PI value = [1-(mean OD sample/mean OD negative control)] x 100

Correlation rates with other commercial ELISA- Random 274 samples from vaccinated group

'P' ELISA 'I' ELISAAniGen CSFV Ab

ELISADifferent results

17 samples 27 samplesCorrelation rate 93.8% 90.0%

Classical Swine Fever virus antibody

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PRRS Ab ELISA 4.0 Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of piglets and growing pigs. The disease is caused by PRRS virus, currently classified as a member of family Arteriviridae, genus Arterivirus. The primary target cell of the virus is alveolar macrophage of pigs. Two major types of the virus exist, the European (EU) and the North America (NA) strain. The virus is primarily transmitted by contaminated feces, urine, semen and infected pigs. High level of sanitary management system is required because fomite infection is also possible.

Quick Procedure

•Routine monitoring of PRRS•PRRS diagnosis of non-vaccinated group•Titer check after vaccination

Indications

Special Features•No singleton reactor (false positive)•Antibodies against European strain, North America

strain and Korean strain can be detected•No cross reaction with other swine disease positive sera

1. Prepara antigen coated micro assay plate2. Dilute test samples and controls with sample diluent (1:39 dilution)3. Add 100㎕ of diluted samples and controls to wells4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate to wells7. Incubate plate for 30 minutes at room temperature8. Wash plate 5 times9. Add 100㎕ of substrate solution and incubate for 15 minutes at room temperature in dark10. Add 100㎕ of stop solution11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm12. S/P value=[OD sample-mean OD negative]/[ mean OD positive- mean OD negative]

Sensitivity and specificity study-AnGen PRRS Ab ELISA 4.0 has high sensitivity, 98.7% and high Specificity, 99.7%

AniGen PRRS Ab ELISA 4.0Positive Negative Total

IFAPositive 324 4 328Negative 1 333 334

Total 325 337 662

Porcine Reproductive and Respiratory Syndrome antibody

•Specimen: Plasma, Serum•Reading time: 75 minutes•Sensitivity: 98.7% vs. IFA•Specificity: 99.7% vs. IFA

23


EB44-04PO PRRS Ab ELISA 4.0 Microplate 480 Wells/Kit

Seroconversion study after challenge of PRRSV

Compare with other commercial ELISAProduct AniGen “I” Commercial vol.2 “I” Commercial vol.3

Test well No. for 1 sample 1 Well 2 Well 1 Well

Time Incubation 30 min. Incubation 30 min. Incubation 30 min.Cut off value S/P 0.4 S/P 0.4 S/P 0.4

Singleton reactor No Yes No

AniGen PRRS Ab ELISA Kit is more sensitive than othersDetection period is earlier than "I" commercial ELISA with no singleton reactor

S/P

ratio

4.0

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0

0 7 13 18 24 29 35 40

AniGen

Commercial Kit A

S/P

ratio

4.0

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0

0 7 13 18 24 29 35 40

AniGen

Commercial Kit A

EU NA

cut off0.4

cut off0.4

* Anigen PRRS Ab ELISA 4.0 can detect PRRS Ab from DPI 7 with PRRS EU and NA strain

-1.0

0

1.0

2.0

3.0

4.0

AniGen

“I” Commercial vol.3

D0

Mea

n S

/P ra

tio

“I” Commercial vol.2

D14 D35

Days post vaccination

<PRRS vaccination DPI results>

1) Antibody response after challenge with PRRS EU, NA strainr

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PED IgA ELISA Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae. PEDV causes acute enteritis in swine of all ages, and it is often fatal for neonatal piglets. To protect piglets from PEDV, Sow transfers immunoglobulin through colostrum to their children until the piglets acquire adaptive immunity. Many reports suggest that IgA is important for protection of PEDV. AniGen IgA ELISA measures preventive anti PED-virus IgA titers of sow and predict defense capability of piglets induced by passive antibody transfer.


EB44-10PO PED IgA Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Quantitative detection of PED IgA antibody•Screening for defensive capacity against PEDV

Indications

Special Features•Easy sample collection•Optimal screening method for defensive capacity of PEDV•Specimen : Colostrum

1. Prepare PED antigen coated test plate2. Dispense 100㎕ of sample diluent into each well3. Dispense 10㎕ of positive, negative control and samples to

each well4. Incubate the plate at 37±1℃ for 60 minutes5. Wash the plate 5 times6. Dispense 100㎕ of diluted enzyme conjugate to each well7. Incubate the wells for 30 minutes at37±1℃

8. Wash the plate 5 times9. Dispense 100㎕ of substrate to each well10. Incubate the wells for 15 minutes at room temperature

(18~25℃)11. Dispense 100㎕ of stopping solution12. Measure the optical density (OD) at 450nm with reference

wavelength at 620nm13. Cut off value = [0.35+ the mean Negative Control absorbance]

Changes in composition of colostrum

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

Colostrum7 14 21 28

Day

Conc

entr

atio

n

IgA

IgG

(The concentration of the IgA is higher than IgG in sow’s milk)

90

80

70

60

50

40

30

20

10

0

Oral IM Control

(Piglet survival rate)(Research in Veterinary Scien ce, Song et al., 2007)

Survival rate of P.O group (IgA)A-farm-OB-farm-O

Survival rate of I.M group (IgG)A-farm-IMB-farm-IM

◈ Inoculated agent : cell adapted DR13 strain◈ Speci�c ELISA was used for IgA detection

Survival rate(%)

Antibody development (in pregnant sows. 2007 research)

Porcine Epidemic Diarrhea virus

•Reading Time : 1 hour and 45 minutes•Survival rate of IgA positive confirmed group

after challenge : see tables below

25

PCV-2 Ab ELISA Porcine Circovirus Type 2, or PCV2, is a very small circular-arranged DNA virus that belongs to the Circoviridae family. PMWS is a serious manifestation of PCV2 infection. PMWS is characterized by severe loss of weight (wasting) and generalized lymph node enlargement. Today we know that there are several PCV2 associated diseases (PCVAD) and severe systemic PCV2 infection remains as the most important manifestation. PCV2-associated Porcine Respiratory Disease Complex (PRDC) is also a very commonly diagnosed PCVAD in the U.S. Less commonly diagnosed diseases include PCV2-associated Enteritis, PCV2- associated Reproductive Failure, and Porcine Dermatitis and Nephropathy Syndrome (PDNS). PCV2-infection is widespread and essentially all pig herds are infected with PCV2 but relatively few have PCVAD. In many cases, PCV2 infection requires a trigger such as co-infection with other pathogens (PRRSV, Mycoplasma hyopneumoniae), immune stimulation of the host, or other stressors to trigger PCV2 infection to progress to PCVAD. Host genetics may also markedly affect the outcome of PCV2 infection and there is increasing evidence of differences in virulence among PCV2 isolates.


EB44-17PO PCV 2 Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Titer check after vaccination•Diagnosis of PCV2 by antibody titration•For prevention of PRDC, PDNS, PMWS

Indications

Special Features•Useful massive screening method for PCV2 •No cross reaction with other swine disease positive sera•Measurement of IgG induced by ORF 2 of PCV2 to predict

outcome of infection

1. Prepare antigen coated micro assay plate2. Dilute test samples and controls with sample diluent

(1:39 dilution)3. Add 100㎕ of diluted samples and controls to wells4. Incubate plate for 30 minutes at room temperature5. Wash plate 5 times6. Add 100㎕ of enzyme conjugate to wells7. Incubate plate for 30 minutes at room temperature8. Wash plate 5 times9. Add 100㎕ of substrate solution and incubate for

15 minutes at room temperature in dark10. Add 100㎕ of stop solution11. Measure the optical density (OD) at 450 nm with

reference wavelength at 620nm12. S/P value = [OD sample-mean OD negative] / [ mean

OD positive- mean OD negative] x 100

0 7 14 21 49 69

VNT5

0 (lo

g 2)

11.5

10.5

9.5

8.5

7.5

6.5

5.5

4.5

1.2

1.0

0.8

0.6

0.4

0.2

0

Opt

ical

den

sity

Days post infection

12.0

10.0

8.0

6.0

4.0

2.0

0.0

6

5

4

3

2

1

00 7 14 21 49 69

VNT5

0 (lo

g 2)

Days p.i.

DN

A c

opie

s/m

l (lo

g 0)Correlation of neutralizing antibody titer with IgG and IgM-Neutralizing antibody (NA) titer was correlated with IgG

*NA: Green line, IgG: Red line, IgM: Blue line (Veterinary Microbiology 125 (2007) 244–255)

Dynamics and viral loads in PCV inoculated pigs-NA has inverse proportion relation with viral load

*NA: Green line, Viral load: Ivory bar (Veterinary Microbiology 125 (2007) 244–255)

•Specimen: Plasma, Serum•Reading time: 75 minutes

Porcine Circovirus 2 antibody

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PCR Kits for Swine disease

Real-Time PCR Kits for Swine disease

•Confirm swine diseases by antigen detection •Differential diagnosis of difficult case shows similar signs•To clarify signs of infection in vaccinated group showing uncertain antibody titer

Indications

Special Features•Ultimate diagnostic system for swine infectious disease •Optimal and unique primers and probes system guarantees highly specific result• Includes positive and negative control•More convenient method than virus culture•One-step PCR using Taqman probe•Enables quantitative and qualitative analysis •Higher detection limit than general PCR : 100~1,000 times more sensitive

Product Diagnosis Cat. No.

Real-Time

PCV-2 Real-Time Detection Kit Porcine Circovirus PD64-03ON

PRRSV Real-Time Detection Kit Porcine Reproductive and Respiratory SyndromeVirus (Differential diagnosis of EU/US strain) PD64-04ON

CSFV Real-Time Detection Kit Classical swine fever virus PD64-05ONInfluenza A (H1N1) Swine Real-Time Detection Kit Novel Influenza A (H1N1) PD64-01ON

SIV Real-Time Detection Kit Original Swine Influenza virus PD64-02ON

•BioNote *PRRS Real-Time Detection Kit →UsefultodifferentiateEUandPRRSVstrain

•BioNote *SIV Real –Time Detection Kit →UsefultodifferentiateSwineInfluenzaVirus(InfluenzaAH1N1)andoriginalSwineInfluenzavirus

Differential Diagnosis

BovineIproduct >>

II RapidITest

28IB.BrucellaIAb29IGS.BrucellaIAb30IBovineITBIAb31IFMDINSPIAb32IBoviD-5IAg

II ELISA

33IB.BrucellaIAbIELISAI2.034ITBIferonIELISA36IBTBIAbIELISAI2.037IFMDINSPIAbIELISA

Bovi

ne

28

Validated from *IZS Umbria e Marche, Peruzia, Italy*Agence Française de Sécurité Sanitaire des Aliments, FranceB.Brucella Ab

Bovine brucellosis is commonly caused by B. abortus and less frequently by B. melitensis, and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Available serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. However, these tests require sample preparation or specialized person and equipment. The Anigen B. Brucella Ab Test Kit provides swift and accurate field test results.


RG23-01DD Rapid B.brucella Ab Device 1 Test x 10/Kit

Test Procedures

•Diagnosis of bovine brucellosis in field•Screening for test and slaughter government policy •Massive screening for animal import & export

Indications

Special Features• Detection of antibodies against Brucella abortus,

melintensis and suis•Standardized by OIE standard sera (B.abortus 1119-3)•More reliable result than rose bengal test•High correlation with ELISA test

C T S

C 2 1B.br

ucell

a Ab

C T S

C 2 1B.br

ucell

a Ab

Slowly add one drop (20㎕) by a capillary tube of sample to the sample well.

Add 3 drops with the bottlecontaining Assay diluent.

3 drops20㎕

Serum, plasma 2 31

Whole blood20 min.

C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Bovine Brucellosis antibody

•World’s first commercialized rapid test kit for detection of B. brucellosis

•Specimen: Blood, Plasma, Serum •Sensitivity: 100% vs. ELISA•Specificity: 99.1% vs. ELISA

Advantages of Anigen Rapid B.Brucella Ab testRBT MRT Anigen

Specimen Serum only Milk only Whole blood, Serum, Plasma Use in field No Yes Yes

Time One day 1 hour 20 minutesCross reaction Cross reaction with Yersinia enterocolitica No cross reaction

* RBT : Rose Bengal Test* MRT : Milk Ring Test

H5

AIV

Ag

29

C T S

C 2 1GS.br

ucell

a AbC T S

C 2 1GS.br

ucell

a Ab

Slowly add one drop (20㎕) by a capillary tube of sample to the sample well.

Add 3 drops with the bottlecontaining Assay diluent.

3 drops20㎕

Serum, plasma 2 31

Whole blood20 min.

GS.Brucella Ab Ovine and caprine brucellosis is commonly caused by B. melitensis and less frequently by B. abortus and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Available serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. However, these tests require sample preparation or specialized person and equipment. The Anigen GS. Brucella Ab Test Kit provides swift and accurate field test results.


RB23-06DD Rapid GS.brucella Ab Device 1 Test x 10/Kit

Test Procedures

•Diagnosis of ovine and caprine brucellosis in field•Screening for test and slaughter government policy •Massive screening for animal import & export

Indications

Special Features•Detection of antibodies against Brucella melintensis,

abortus and suis•More reliable result than rose bengal test•High correlation with ELISA test•World’s first commercialized rapid test kit for detection of

GS. brucellosis

C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Goat & Sheep Brucellosis antibody

•Specimen: Blood, Plasma, Serum, Milk•Sensitivity: 98% vs. ELISA•Specificity: 100% vs. ELISA

Advantages of Anigen Rapid GS.Brucella Ab testRBT MRT Anigen

Specimen Serum only Milk only Whole blood, Serum, Plasma Use in field No Yes Yes

Time One day 1 hour 20 minutesCross reaction Cross reaction with Yersinia enterocolitica No cross reaction

* RBT : Rose Bengal Test* MRT : Milk Ring Test

H5

AIV

Ag

Bovi

ne

30

Bovine TB Ab Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the basis of delayed hypersensitivity reactions. Subclinical infection is common and clinical signs are non-specific in many cases. A sensitive and accurate diagnostic tool is required because purified protein derivative (PPD) test has only a 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other mycobacterium infection (eg; M.avium) should be distinguished with this method. The MPB 70 protein was revealed to be a highly species specific protein secreted by Mycobacterium bovis. Anigen rapid bovine TB Ab test kit has been developed using MPB70 protein with a immunochromatographic assay method.


RB23-02DD Rapid Bovine TB Ab Device 1 Test x 10/Kit

Test Procedures

•Search out BTB carrier in field•Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-

handed situation• CombinewithPPDorIFN-γassayineradicationsystem

Indications

Special Features•No cross reaction against other mycobacterium species (M. avium)• Easy to use, saving time and labor• Specimen: Blood, Plasma, Serum• Sensitivity: 81.7% vs. PPD test, 84.2% vs. “E” commercial ELISA• Specificity: 91.4% vs. PPD test, 84% vs. “E” commercial ELISA

BTB

Ab

S

C T

BTB

Ab

S

C T

Add 1 drop of the mixed sample and wait for another 1 minute.

Add 3 drops of developing buffer.

3 drops

Whole blood

4 5

3 drops 1 drop+

Add 3 drops of whole blooddiluent into the test tube.

2 Add 1 drop of whole blood into the test tube and wait for 1 minutes to mix it.

3

Plasma or serum

1 drop (10㎕)Whole blood : Start from Step 2Plasma or serum : Start from Step 4

1

1 min.

20 min.C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Mycobacterium bovis antibody

Imm

une

Resp

onse

Tuberculin Skin TestAntibody Response

Gamma InterferonTest (IFN-γ)

Aner

gy

AniGen TB feron ELISA Test Kit

PPD Test

Anigen Rapid Bovine TB Ab Test Kit

Anigen Rapid BTB Ab Test Kit detects mid to late (anergy) stage of TB affected cattle by immunochromatographic assay (Applicable in field assay)Generally, M.bovis Ab is detectable 90 to 100 days after infection

Immune development of bovine tuberculosis

31

FMD NSP Ab Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer, and water buffalo. In many countries the disease is controlled by vaccinations that consist of (partly) purified structural proteins (SP) of the FMD virus, and therefore vaccinated animals only elicit antibodies directed against the structural proteins. Non structural protein (NSP) is expressed only by replicating viruses, and inactivated vaccines are purified to remove the cellular proteins and NSP. Therefore, only animals that have been infected with wild type develop antibodies against NSP. It is important to differentiate SP and NSP antibodies in countries that use vaccination to control FMDV outbreaks to discriminate wild type infections and immune response to vaccination.


RB28-02DD Rapid FMD NSP Ab Device 1 Test x 10/Kit

Test Procedures

•To discriminate between infection and vaccination•For field diagnosis of FMD in non-vaccinated herds•Tentative diagnosis for swift control in outbreak suspected situation

Indications

Special Features•No cross reaction with vaccinated group•Specimens: Blood, Plasma, Serum•Applicable to all artiodactyl mammals (Cattle, Sheep, Goat, Pig)•Sensitivity: 95.4% vs. Commercial ELISA•Specificity: 100% vs. Commercial ELISA

FMD

NSP

Ab

S

C T

FMD

NSP

Ab

S

C T

Add 1 drop of the mixed sample and wait for another 1 minute.

Add 3 drops of developing buffer.

3 drops

Whole blood

4 5

3 drops 1 drop+

Add 3 drops of whole blooddiluent into the test tube.

2 Add 1 drop of whole blood into the test tube and wait for 1 minutes to mix it.

3

Plasma or serum

1 drop (10㎕)Whole blood : Start from Step 2Plasma or serum : Start from Step 4

1

1 min.

15 min.

C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Food and mouth disease virus antibody

Bovi

ne

32

BoviD-5 Ag

Newborn calves are susceptible to neonatal calf diarrhea especially during their first 3~4 weeks of life. Bacteria, viruses and parasites, by attacking the lining of the calf`s intestine, give rise to diarrhea. It is one of the major financial damage factors in bovine farms, because once infected this decreases the absorption of essential nutrients from milk and leads to weight loss and dehydration. Generally, calf diarrhea is considered as an emergency situation that requires swift diagnosis and rehydration. One of the key features of a rapid test kit is the "rapidity" of the test result, thus BoviD- 5 Ag is an ideal test in these situations.

Ordering Information

Test Procedures

•For differential diagnosis of calf diarrhea•Calf diarrhea etiologic agent monitoring in field•For immediate treatment required cases

Indications

Special Features•Useful tool for ruling out the cause of calf diarrhea•Specimen : Diarrheal feces•No cross reaction with other etiologic agents of calf diarrhea•Sensitivity and Specificity : see table

Giardia Ag

or

Collect the samples from canine feces using the swab.1

4 5

2



4 drops


5~10min. C T

C T

C T

C T

Negative

Positive

Invalid

Interpretation

Bovine Rota, Corona, E.coli K99, Cryptosporidium, Giardia antigen



Sensitivity SpecificityCryptosporidium 98.2% 99.0%

vs.PCRRotavirus 99.0% 98.0%

Coronavirus 98.4% 98.0%E.coli K 99 97.8% 99.0%

Giardia 92.1% 99.1% vs.ELISA

Cat. No. Description Type Packing sizeRG13-02DD Rapid BoviD-5 Ag Device 1 Test x 10/KitRG13-01DD Rapid BoviD-4 Ag Device 1 Test x 10/Kit

33

B.Brucella Ab ELISA 2.0 Bovine brucellosis is commonly caused by Brucella abortus and less frequently by B. melitensis, and rarely by B. suis. Humans may be infected by contact with animals or animal products contaminated with these bacteria. Serological tests include the Rose Bengal, ELISA, complement fixation test and tube agglutination test. Anigen B.brucella Ab ELISA Test Kit is a serology test that provides high sensitivity and specificity with bovine milk and serum samples.


EB43-01PO B.brucella Ab ELISA 2.0 Microplate 480 Wells/Kit

Quick Procedure

•Diagnosis of bovine brucellosis in lab•Screening for test and slaughter government policy •Massive screening for bovine import & export market

Indications

Special Features•Fully meets the requirement of EU Council Directive and the

OIE Manual •Standardized by OIE standard sera (B.abortus 1119-3)•No cross reaction with yersinia enterocolitica•Specimen: plasma, serum, milk•Reading time: 1 hour and 45 minutes•Sensitivity: serum 100%, milk 100% (Vs Commercial ELISA)•Specificity: Serum 97.9 %, milk 99.1% (Vs Commercial ELISA)

1. Prepare LPS coated test plate2. Dilute test samples 1:50 with sample diluent

(Do not dilute controls and Milk)3. Add 100㎕ of positive control, negative control, and diluted

test samples4. Incubate the plate for 60 minutes at 37℃

5. Wash plate 5 times6. Add 100㎕ of diluted enzyme conjugate to wells7. Incubate plate for 30minutes at 37℃

8. Wash plate 5 times9. Add 100㎕ of mixed substrate (Ready to use) to each well and

incubate for 15 minutes at room temperature10. Add 100㎕ of stopping solution11. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm12. %P value=[OD sample/mean OD positive] x 100

(1)Study in serum Specimens :Total 271 of positive and negative cow serum from KNVRQS*

AniGen ELISA + -

Other commercial ELISA

+ 127 0 127- 3 141 144

130 141

Result

Sensitivity 100%Specificity 97.9%

(2) Study in raw milk Specimens :Total 271 of positive and negative cow milk from KNVRQS*

AniGen ELISA + -

Other commercial ELISA

+ 68 0 68- 1 116 117

69 116

Result

Sensitivity 100%Specificity 99.1%

Bovine Brucellosis antibody

Bovi

ne

34

TB feron ELISA Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. The infection is often subclinical; even when present, clinical signs are not specifically distinctive of this disease and might include weakness, anorexia, emaciation, enlargement of lymphnodes, and cough. Routine screening can be time and labor intensive. The Anigen TB-feron is an indirect Enzyme Linked ImmunosorbentAssayforthequantitativedetectionofinterferongamma(IFN-γ).IFN-γassayisbasedonthefactthatananimalhasbloodlymphocyteswhichcanmemorize stimulating antigen immunologically when it is stimulated by exogenous or endogenous antigens. When an antigen is added to the blood from a primed animal within a tube, antigen specific effector/memory T cell are rapidly re-stimulatedtoproduceIFN-γ,thecytokine,whichisusedasaspecificmarker incell-mediated immune response (recall response). It is an alternative test method of intradermal skin test (PPD) because to its easy procedure and better sensitivity compared to the skin test.

Quick Procedure

•Quantitative detection of interferon gamma in bovine plasma

•Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-

handed situation•Combine with ELISA or Rapid test in eradication system

Indications Special Features•High reliable result than PPD•OIE standard for BTB diagnosis•GoodcorrelationwithotherIFN-γassay•Provide antigens for sensitization•Specimen : Stimulated plasma•Reading time : 2 hours •Concordance rate: 100% vs. “B” commercial ELISA

1. Add 100㎕ of sample diluent to each well2. Add 50㎕ of controls and each of prepared samples

(Bovine PPD stimulated plasma Avian PPD stimulated plasma, PBS stimulated plasma) to each well3. Incubate the wells at 37℃ for 30 minutes4. Wash the wells 5 times5. Add 100㎕ of prepared amplification conjugate to each well6. Repeat step 3 and 47. Add 100㎕ of prepared enzyme conjugate to each well8. Repeat step 3 and 49. Add 100㎕ of substrate to each well10. Incubate the wells for 30 minutes at room temperature11. Add 100㎕ of stopping solution to each well. Reading W/L 450nm (Ref. W/L 620nm)12. OD value: OD bovine PPD-PBS and OD bovine PPD-avian PPD

Sample preparation

Stimulating antigen (Sensitization should be performed within 30 hours after blood collection)100㎕- Bovine PPD- Avian PPD- PBS (Negative control)Plasma 3ml

Overnightincubation

for 16 hoursCentrifuge

Take supernatant

liquid

TB feronELISA

testing+

Gamma interferon assay for Mycobacterium bovis

35

Field benefits of the IFN-γ assay•Animals retested as often as required (no interference with the immune status of animal)•Double handing of cattle avoided•Better sensitivity compared to skin test•Reduced need for comparative intradermal test since both avian and bovine PPDs are used

Lab benefits of the IFN-γ assay•Results obtained within 24 hours•A lab test is subject to quality control, standard procedures and objective interpretation•Suitably adapted to epidemiological characteristics of the territory

Performance1. Comparative study with other IFN test

AniGen TB feronTotal

(-) (+)

"B" ELISA(-) 87 0 87 Correlation 100% 142/167(+) 0 12 12 Sensitivity 100% 12/12

Total 87 12 99 Specificity 100% 87/87

2. Comparative study with skin test (PPD)

AniGen TB feronTotal

(-) (+)

PPD(-) 50 0 50 Correlation 85% 142/167(+) 25 92 117 Sensitivity 78.6% 92/117

Total 75 92 167 Specificity 100% 50/50

Ordering InformationCat. No. Description Type Pack ingsize

EG38-01PO TB feron ELISA Microplate 480 Wells/Kit (150 tests)

Imm

une

Resp

onse



Aner

gy


AniGen BTB Ab ELISA 2.0 Test Kit

PPD Test

AniGen TB feron ELISA detects early to mid stage of TB affected cattle by cellular immunity measurement


Bovi

ne

36

BTB Ab ELISA 2.0 Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the basis of delayed hypersensitivity reactions. Subclinical infection is common and clinical signs are non-specific in many cases. A sensitive and accurate diagnostic tool is required because purified protein derivative (PPD) tests have been known to have 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other mycobacterium infection (eg; M.avium) should be distinguished with this method. The MPB 70 protein has been revealed to be a highly species specific protein secreted by Mycobacterium bovis. Anigen BTB Ab ELISA 2.0 test kit has been developed using MPB70 protein in an Enzyme Linked Immunosorbent Assay method. The serological diagnostic test is suitable for massive screening of bovine tuberculosis to detect mid to late (anergy) state cattle.


EB43-04PO BTB Ab ELISA 2.0 Microplate 480 Wells/Kit

Quick Procedure

•Search out BTB carrier •Screening for test and slaughter government policy•First step for massive screening instead of PPD in short-handed situation• CombinewithPPDorIFN-γassayineradicationsystem

Indications

Special Features•No cross reaction against other mycobacterium species (M. avium)•Quantitative detection of M. bovis antibody•The world’s first reliable BTB Ab ELISA for serological diagnosis

1. Prepare BTB antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of M. bovis antigen-HRP to each well 4. Incubate plate for 60 minutes at 37℃

5. Wash plate 6 times6. Add 100㎕ of mixed substrate solution (Ready to use)

to each well and incubate for 15 minutes at room temperature

7. Add 100㎕ of stopping solution to each well 8. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm9. S/P value=[OD sample-mean OD negative)] / [mean OD

positive – mean OD negative] x 100

Imm

une

Resp

onse



Aner

gy


AniGen BTB Ab ELISA 2.0 Test Kit

PPD Test

AniGen BTB Ab ELISA 2.0 detects mid to late (anergy) stage of TB affected cattle by antibody titer check againt TB (Laboratory method for antibody check)Generally, M.bovis Ab is detectable 90 to 100 days after infection

Mycobacterium bovis antibody

•Reading time: 75 minutes•Specimen: Serum•Sensitivity: 88.1% vs. PPD test•Specificity: 99.2% vs. PPD test


37

FMD NSP Ab ELISA Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer, and water buffalo. In many countries the disease is controlled by vaccinations that consist of (partly) purified structural proteins (SP) of the FMD virus, and therefore vaccinated animals only elicit antibodies directed against the structural proteins. Non structural protein (NSP) is expressed only by replicating viruses, and inactivated vaccines are purified to remove the cellular proteins and NSP. Therefore, only animals that have been infected with wild type develop antibodies against NSP. It is important to differentiate SP and NSP antibodies in countries that use vaccination to control FMDV outbreaks to discriminate wild type infections and immune response to vaccination.


EB48-01PO FMD NSP Ab ELISA Microplate 480 Wells/Kit

Quick Procedure

•Discriminate sera between infection and vaccination•Diagnosis of FMD in non-vaccinated herds•Screening for test and slaughter government policy

Indications

Special Features•Differential test of FMD infected or vaccinated•High accuracy equivalent to a world standard ELISA kit•Easy test procedure: No serum pre-dilution required•Cost effective: No requirement for an uncoated

microplate for serum pre-dilution

1. Prepare FMD NSP antigen coated test plate2. Add 50㎕ of controls and sample to wells3. Add 50㎕ of diluted enzyme conjugate to wells4. Incubate plate for 90 minutes at 37℃

5. Wash plate 6 times6. Dispense100㎕ of substrate (Ready to use) into each well

and incubate for 15 minutes at room temperature7. Add 100㎕ of stopping solution8. Measure the optical density (OD) at 450 nm with reference

wavelength at 620nm9. PI value=[1-(OD sample/mean OD negative)] x 100

Reactivity of AniGen FMD NSP Ab ELISA for sera originated from vaccinated animals

AniGen FMD NSP Ab ELISA detects antibodies against nonstructural protein from 7 days to 7 months after infection(*Experimentally contact challenge animal group, The performance evaluation was performed in OIE FMD Ref. Laboratory)

AniGen 0% 76.1% 93.8% 50%Checkit 0% 38.3% 93.8% 20%

0

20

40

60

80

100

AniGen

Checkit

5 days 7 days 14~26 days 7 months

Food and mouth disease virus antibody

Validated from *FGI «ARRIAH», Federal Center For Animal Health, Russia

•Species: Cattle, Sheep, Goat, Pig•Specimen: Plasma, Serum•Reading time : 1 hour and 45 minutes•Sensitivity: Cattle 93.8%•Specificity: Cattle 99.9%, Pig 99.9%, Sheep & goat 100%

Current available product listRapid

Species Product Cat. No. Type Packing size Description Sample

PETAnimal

Rapid CPV Ag Test Kit

Rapid CHW Ag Test Kit 2.0

Rapid CDV Ag Test Kit

Rapid CCV Ag Test Kit

Rapid CPV Ag/CCV Ag Test Kit

Rapid CIV Ag Test Kit

Rapid CDV Ag/CAV Ag Test Kit

Rapid CIRD-3 Ag Test Kit

Rapid CDV Ag/CIV Ag Test Kit

Rapid CPV/CCV/Giardia Ag Test Kit

Rapid Rabies Ag Test Kit

Rapid Rota Ag Test Kit

Rapid Giardia Ag Test kit

Rapid CPV Ab Test Kit 2.0

Rapid CDV Ab Test Kit 2.0

Rapid Canine brucella Ab Test Kit

Rapid Leishmania Ab Test Kit 2.0

Rapid E.canis Ab Test Kit

Rapid Leptospira Ab Test Kit

Rapid Canine Total IgE Test Kit

Rapid CaniV-4 Test Kit

Rapid Anaplasma Ab Test Kit

Rapid Lyme Ab Test Kit

Rapid E.canis Ab/Anaplasma Ab Test Kit

Rapid Anaplasma Ab/Lyme Ab Test Kit

RG11-01DD

RG11-02DD

RG11-03DD

RG11-04DD

RC11-05DD

RG11-07DD

RC11-08DD

RC11-09DD

RC11-10DD

RC11-12CC

RG18-01DD

RG18-03DD

RG18-04DD

RB21-51DD

RB21-52DD

RB21-03DD

RB21-04DD

RB21-05DD

RB21-07DD

RB21-10DD

RC21-20DD

RB21-21DD

RB21-22DD

RC21-23DD

RC21-24DD

Device

Device

Device

Device

Device (Dual)

Device

Device (Dual)

Device (Dual)

Device (Dual)

Device (Triple)

Device

Device

Device

Device

Device

Device

Device

Device

Device

Device

Device (Pento)

Device

Device

Device (Dual)

Device (Dual)

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 5/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

Detection of Canine Parvovirus antigen

Detection of Dirofilaria immitis antigen

Detection of Canine Distemper Virus antigen

Detection of Canine Coronavirus antigen

Detection of Canine Parvovirus & Canine Coronavirus antigen

Detection of Canine Influenza Virus antigen

Detection of Canine Distemper Virus & Canine Adenovirus antigen

Detection of Canine Distemper Virus, Canine Adenovirus & Canine Influenza Virus antigen

Detection of Canine Distemper Virus & Canine Influenza Virus antigen

Detection of Canine Parvovirus, Canine Coronavirus & Giardia lamblia antigen

Detection of Rabies Virus antigen

Detection of Rotavirus antigen in cattle, pig, and dog

Detection of Giardia lamblia antigen

Titration of Canine Parvovirus antibody

Titration of Canine Distemper Virus antibody

Detection of Brucella canins antibody

Detection of Leishmania infantum antibody

Detection of Ehrlichia canis antibody

Detection of Leptospira interrogans antibody

Detection of Total IgE

Detection of Dirofilaria immitis antigen & Anaplasma, Borrelia, Ehrlichia antibody

Detection of Anaplasma phagocytophilum and platys antibody

Detection of Borrelia burgdorferi antibody

Detection of Ehrlichia canis, Anaplasma phagocytophilum/platys antibody

Detection of Anaplasma phagocytophilum/platys, Borrelia burgdorferi antibody

Feces

Whole blood, plasma or serum

Conjunctiva, feces, saliva, urine, plasma, or serum

Feces

Feces

Conjunctiva and nasal swab




Feces

Brain homogenates

Feces

Feces







Plasma or serum






Rapid FeLV Ag Test Kit

Rapid FPV Ag Test Kit

Rapid FIV Ab/FeLV Ag Test Kit

Rapid FHW Ag/Ab Test Kit

Rapid FeliCheck-3 Test Kit

Rapid FCoV Ag Test Kit

Rapid FIV Ab Test Kit

Rapid FCoV Ab Test Kit

Rapid Toxoplasma Ab Test Kit

RG12-01DD

RG12-03DD

RC12-04DD

RC12-05CD

RC22-10CD

RG12-05DD

RB22-01DD

RB22-03DD

RB22-06DD

Device

Device

Device (Dual)

Device (Dual)

Device (Triple)

Device

Device

Device

Device

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

Detection of Feline Leukemia Virus antigen

Detection of Feline Panleukopaenia Virus antigen

Detection of Feline Leukemia Virus & Feline Immunodeficiency Virus antibody

Detection of Feline Heratworm Antigen & antibody

Detection of Feline Leukemia Virus, Feline Immunodeficiency Virus & Dirofilaria immitis antibody

Detection of Feline Coronavirus antigen

Detection of Feline Immunodeficiency Virus antibody

Detection of Feline Corona Virus antibody

Detection of Toxoplasma gondii antibody


Feces




Feces




LivestockAnimal

Rapid Bovi D-4 Ag Test Kit

Rapid Bovi D-5 Ag Test Kit

Rapid Bovine Coronavirus Ag Test Kit

Rapid Cryptosporidium Ag Test Kit

Rapid Rota Ag Test Kit

Rapid Bovine Brucella Ab Test Kit

Rapid Bovine TB Ab Test Kit

Rapid FMD NSP Ab Test Kit

RC13-01DD

RC13-02DD

RG13-03DD

RG13-04DD

RG18-03DD

RB23-01DD

RB23-02DD

RB28-02DD

Device (Pento)

Device (Pento)

Device

Device

Device

Device

Device

Device

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium antigen

Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium, Giardia antigen

Detection of Bovine Coronavirus antigen

Detection of Bovine Cryptosporidium antigen

Detection of Rotavirus antigen in cattle, pig, and dog

Detection of Brucella abortus antibody in cattle

Detection of Mycobacterium bovis antibody

Detection of Foot and Mouth Ddisease Vvirus antibody field infected

Feces

Feces

Feces

Feces

Feces


Plasma, serum


Rapid GS. Brucella Ab Test Kit

Rapid GS. Brucella Ab Test Kit

Rapid Toxoplasma Ab Test Kit (For farm animals)

RB23-06DD

RB23-06SG

RB28-05DD

Device

Strip

Device

1Test x 10/Kit

1Test x 25/Kit

1 Test x 10/Kit

Detection of Brucella antibody in sheep and goat

Detection of Brucella antibody in sheep and goat

Detection of Toxoplasma gondii antibody




Rapid MERS-CoV Ag Test Kit

Rapid Camel Brucella Ab Test Kit

RG18-05SG

RB23-09SG

Strip

Strip

1Test x 25/Kit

1Test x 25/Kit

Detection of Middle East Respiratory Syndrome Coronavirus antigen

Detection of Brucella antibody in camel

Nasal, nasosopharyngeal swab or aspirates


Rapid PED Ag Test Kit

Rapid TGE Ag Test Kit

Rapid TGE Ag/PED Ag Test Kit

Rapid SIV Ag Test Kit

Rapid PED Ag/Rota Ag Test Kit

Rapid FMD NSP Ab Test Kit

RG14-01DD

RG14-02DD

RG14-03DD

RG14-04DD

RG14-05DD

RB28-02DD

Device

Device

Device (Dual)

Device

Device (Dual)

Device

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 10/Kit

Detection of Porcine Epidemic Diarrhea Virus antigen

Detection of Transmissible Gastroenteritis Virus antigen

Detection of TGE and PED Virus antigen

Detection of Swine Influenza Virus antigen

Detection of PED and Rotavirus antigen

Detection of Foot and Mouth Disease Virus antibody field infected

Feces

Feces

Feces

Nasal fluid or tracheal swab and lung tissue

Feces


Rapid AIV Ag Test Kit

Rapid NDV Ag Test Kit

Rapid IBDV Ag Test Kit

Rapid H5 AIV Ag Test Kit



Rapid AIV Ag/NDV Ag Test Kit

Rapid IBV Ag Test Kit

Rapid AIV Ab Test Kit

RG15-01DG

RG15-03DD

RG15-04DD

RG15-06DG

RG15-07DG

RG15-08DG

RC15-09DD

RG15-13DD

RB25-01MH

Device

Device

Device

Device (3lines)

Device (3lines)

Device (3lines)

Device (Dual)

Device

Device (Multi)

1Test x 25/Kit

1Test x 10/Kit

1Test x 10/Kit

1Test x 25/Kit

1Test x 25/Kit

1Test x 25/Kit

1Test x 10/Kit

1Test x 10/Kit

10Tests x 3/Kit

Detection of Avian Influenza type A Virus antigen

Detection of Newcastle Disease Virus antigen

Detection of Infectious Bursal Disease Virus antigen

Detection of Avian Influenza type A and subtype H5 Virus antigen



Detection of Avian Influenza type A and Newcastle Disease Virus antigen

Detection of Infectious Bronchitis Virus antigen

Detection of Avian Influenza Virus Type A antibody

Feces or Trachea swab


Bursa of Fabricious, Feces






Plasma, serum

For further information, please scan this QR-code on your mobile.

" "ELISA

Species Product Cat. No. Type Packing size Description SampleBovine Brucella Ab ELISA

BTB Ab ELISA

FMD NSP Ab ELISA

TB feron ELISA

EB43-01PO

EB43-04PO

EB48-01PO

EG38-01PO

Microplate

Microplate

Microplate

Microplate

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

150 Tests/Kit

Detection of Brucella abortus antibody

Detection of Mycobacterium bovis antibody

Detection of Foot and Mouth Disease Virus antibody field infected

Detection of Gamma-interferon in bovine tuberculosis

Serum, plasma, raw milk

Serum, plasma

Serum, plasma

Plasma

CSFV Ab ELISA

PRRS Ab ELISA 4.0

PED IgA ELISA

PCV2 Ab ELISA

EB44-13PO

EB44-04PO

EB44-10PO

EB44-17PO

Microplate

Microplate

Microplate

Microplate

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

Detection of Classical Swine Fever Virus antibody

Detection of Porcine Reproductive and Respiratory Syndrome Virus antibody

Detection of IgA for Porcine Epidemic Diarrhea Virus

Detection of Porcine Circovirus antibody

Serum, plasma

Serum, plasma

Colostrum

Serum, plasma

AIV Ab ELISA

H5 AIV Ab ELISA

NDV Velo Ab ELISA

IBV Ab ELISA

NDV Ab ELISA

IBDV Ab ELISA

EB45-02PO

EB45-03PO

EB45-01PO

EB45-05PO

EB45-06PO

EB45-07PO

Microplate

Microplate

Microplate

Microplate

Microplate

Microplate

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

480 Wells/Kit

Detection of AIV type A antibody

Detection of AIV subtype H5 antibody

Detection of velogenic Newcastle Disease Virus antibody

Detection of Infectious Bronchitis Virus antibody

Detection of Newcastle Disease Virus antibody

Detection of Infectious Bursal Disease Virus antibody

Serum

Serum

Serum

Serum

Serum

Serum

Conventional PCRSpecies Product Cat. No. Type Packing size Description Sample

LivestockAnimal

AIV Detection Test Kit

H5 AIV Detection Test Kit



Lento NDV Detection Test Kit

Velo NDV Detection Test Kit

IBDV Detection Test Kit

PD55-01ON

PD55-02ON

PD55-03ON

PD55-04ON

PD55-07ON

PD55-08ON

PD55-11ON

Conventional PCR

Conventional PCR

Conventional PCR

Conventional PCR

Conventional PCR

Conventional PCR

Conventional PCR

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

Detection of AIV type A

Detection of AIV subtype H5



Detection of low pathogenci NDV (Lentogenic)

Detection of high pathogenic NDV (Velogenic)

Detection of Infectious Bursal Disease Virus

Sercetion, organ, blood, feces, fluid etc.







Real-Time PCRSpecies Product Cat. No. Type Packing size Description Sample

LivestockAnimal

AIV A Real-Time Detection Test Kit

H5 AIV Real-Time Detection Test Kit



REV Real-TIme Detection Test Kit

MDV Real-TIme Detection Test Kit

Lento NDV Real-Time Detection Test Kit

Velo NDV Real-Time Detection Test Kit

IBV Real-Time Detection Test Kit

TRT Real-Time Detection Test Kit

IBDV Real-Time Detection Test Kit

REO Real-Time Detection Test Kit

MYCO G/S Real-Time Detection Test Kit (2 Wells type)

ALV Real-Time Detection Test Kit

AIV H5/N1 Duplex Real-Time Detection Test Kit

PD65-01ON

PD65-02ON

PD65-03ON

PD65-04ON

PD65-05ON

PD65-06ON

PD65-07ON

PD65-08ON

PD65-09ON

PD65-10ON

PD65-11ON

PD65-12ON

PD65-14ON

PD65-15ON

PD65-51ON

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

Detection of AIV type A




Detection of Reticuloendotheliosis Virus

Detection of Marek's disease Virus

Detection of low pathogenci NDV (Lentogenic)

Detection of high pathogenic NDV (Velogenic)

Detection of Infectious Bronnchitis Virus

Detection of Infectious Turkey Rhinotracheitis

Detection of Infectious Bursal Disease Virus

Detection of Avian Reovirus

Detection of Avian Mycoplasma gallisepticum, and M. iowae, M. meleagridis , M. synoviae

Detection of Avian Leukosis Virus

Detection of AIV H5 and N1
















SIV Real-Time Detection Test Kit (2 Wells type)

PCV-2 Real-Time Detection Kit

PRRSV Real-Time Detection Kit (2 Wells type)

CSFV Real-Time Detection Kit

PD64-02

PD64-03

PD64-04

PD64-05

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

Detection of Swine Influenza Virus

Detection of Procine Circovirus

Detection of Procine Reproductive and Respiratory Syndrome Virus

Detection of Classical Swine Fever Virus





BTV Real-Time Detection Kit

IBR Real-Time Detection Kit

BVDV Real-Time Detection Kit

FMD Real-Time Detection Kit

PD63-03

PD63-04

PD63-05

PD68-01

Real-Time PCR

Real-Time PCR

Real-Time PCR

Real-Time PCR

96Tests/Kit

96Tests/Kit

96Tests/Kit

96Tests/Kit

Detection of Bluetongue Virus

Detection of Infectious Bovine Rhinotracheitis Virus

Detection of Bovine Viral Diarrhea Virus

Detection of Foot and Mouth Disease Virus





│Ver.4.1Product Catalog

CIA 15-04E1

Livestock

Documents

Livestock Product Catalog - lab- · PDF file7 AIV Ab Wild birds and some poultry infected by HPAI show no clinical signs, and their virus shedding amount is too small to be detected