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Control pO-PLD2-LO11pO-PLD2-LO19pA-PLD2-LA3pA-PLD2-LA11

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C LA11 LA19 C LA11 LA19 C LA11 LA19 C LA11 LA19 C LA11 LA19

Supplemental Figure 2. Altered Expression of PLD2 Result in Altered Auxin Effects on Primary Root Growth.

A. Compared with controls (C), PLD2-overexpressing plants were hypersensitive to auxin treatment (upper panel), whereas those deficient in were less sensitive (lower panel). Nine-day-old plants were employed for experiments. Arrows highlight differences in primary root length. Bar=1 cm.

B. Measurement of primary root lengths of control and transgenic plants with altered expressions of PLDζ2 grown on medium supplemented with various concentrations of IAA for 9 days confirmed hypersensitivity of PLD2-overexpressing plants to auxin and relative insensitivity of PLD2-deficient plants. Error bars indicate SE (n>40) and statistical analysis was performed using a one-tailed Student’s t test (*, P<0.01).

Li and Xue, Supplemental Figure 2