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Page 1: Think Outside the Cell

Think Outside the Cell

Page 2: Think Outside the Cell

Passenger: Type III Needle scFvTypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex

Functional Assay:•Incubate DH10B cells with his-tagged espA• Wash and lyse cells•Dry lysate on nitrocellulose, block, and stain with anti-his antibody•Negative controls:

Results: •Displayers only:•Displayers + Needle scFv :

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Dot blot of …legend•ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1


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