Upload
chesmu
View
41
Download
0
Embed Size (px)
DESCRIPTION
Display-O-Matic. Think Outside the Cell. Display-O-matic. Passenger: Type III Needle scFv. TypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex Functional Assay: Incubate DH10B cells with his-tagged espA Wash and lyse cells - PowerPoint PPT Presentation
Citation preview
Think Outside the Cell
Passenger: Type III Needle scFvTypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex
Functional Assay:•Incubate DH10B cells with his-tagged espA• Wash and lyse cells•Dry lysate on nitrocellulose, block, and stain with anti-his antibody•Negative controls:
Results: •Displayers only:•Displayers + Needle scFv :
Display-O-matic
1 2 3 4 5 6 7 8
1
2
3
4
5
6
7
8
9
Dot blot of …legend•ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1