Cyprus conference, 2016
Vermicomposting agricultural waste into value-p g gadded products: 30yr’s R & D in China
Zh j SUNZhenjun SUN
China Agricultural University Beijing Vermitech Institute
Email:[email protected]
College of Resources and Environmental Sciences, CAU
@
Cyprus conference, 2016The Status of Agricultural Waste Utilization in China
Total agricultural waste resources:1 billion tons, includingg , gCrop straws: 0.7 billion tons; livestock manures: 0.18 billion tons
Manure fertilizer10%
Directly returning to field10% returning to field
50%
Bi
Directly returning to field
Burning 40%
Biogas production 3%
returning to field20%
strawsmanures
elop
ed
reaIndustrial
Organic fertilizer 5%
manures
U t t d 40D
eve armaterials 3%
Surplus 30-40% Surplus 70 Environmental
Untreated 40-45%
College of Resources and Environmental Sciences, CAU
Surplus 70-80%
Environmental pollution
Cyprus conference, 2016
A i lt l Ci l E D l t P tt
Coordination
Agricultural Circular Economy Development Pattern
Agr Small Conventional Live Resource
Coordination
ricultu
Internal circulation
Circular
agriculture
Ecological Production Environmentural w
L t l
Circular agriculture
gagriculture
Agriculture and
ecology
Life
Society
Economy
waste
Large external circulation
Agriculture andindustry combination
Life Economy
Circular societyCollege of Resources and Environmental Sciences, CAU
Circular society
Cyprus conference, 2016 Use strategy
MethodsProblems ResultsMethodsProblems Results
LLarge Quantity Efficient Quantity
Poor Quality
Highquality
QualityQuality
Low value Industrial Value
Harmful Recycle Harm
College of Resources and Environmental Sciences, CAU
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Ecological UtilizationStraw returning
Methane productionMethane production
Edible fungig
Vermicomposting
Agriculture and livestock
composting
Multiple integrationCollege of Resources and Environmental Sciences, CAU
Multiple integration
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Hi h Q lit UHigh Quality Use
Commercialization and Marketization
New products:
Bi l i l i (Bi l i l f iliBiological preparation(Biological fertilizer、
Biological pesticides Etc )Biological pesticides Etc.)
Biochemical preparation(Amino acidsBiochemical preparation(Amino acids、
Enzyme、Oligosaccharides Etc.)College of Resources and Environmental Sciences, CAU
Enzyme、Oligosaccharides Etc.)
Cyprus conference, 2016产品
15000
16000
产品
12000
14000
60008000
10000
750
4000
6000
150 300750
0
2000
原料 传统堆肥 商品有机肥 叶面肥或酶制剂 木寡糖或抗菌肽原料 传统堆肥 商品有机肥 叶面肥或酶制剂 木寡糖或抗菌肽
Comparison of the added value of the products developedComparison of the added value of the products developed from agricultural wastefrom agricultural waste
College of Resources and Environmental Sciences, CAUComparison of the added value of the products developedComparison of the added value of the products developed from agricultural waste from agricultural waste
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Vermiculture in China
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
f ff i i l i ldfactors affecting vermiculture yield thi kmaterials thickness density
residuals→ earthworm→
Vermiculture
earthworm→ vermicast→
harvestconditions Bed design
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016Simple windrow system p y
大田养殖大田养殖
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
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Plastic Greenhouse System in North China
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College of Resources and Environmental Sciences, CAU
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College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
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Composting pretreatment
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College of Resources and Environmental Sciences, CAU
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College of Resources and Environmental Sciences, CAU
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Intercroping system:p g yvermiculture in field
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College of Resources and Environmental Sciences, CAUIntercropping of vermicomposting and banana
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College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
A ib i l id i h Ei i F idAntibacterial peptides in earthworm Eisenia FetidaSun (1995) found an antibacterial peptide that was composed of
f i id f h fi i h ldforty amino acids from earthworm at first in the world. Name Sequence of amino
acidNumber of amino acid
Molecular weight
Antibacterial characteristicacid amino acid weight characteristic
EP1 / 40 4832 Bacteria and fungi resistance
EP2 Ac-Ala-Met-Val-Ser-Ser 6 535.27 Bacteria resistance
EP3 Ac-Ala-Met-Val-Gly-Thr 6 519.27 Bacteria resistancey
EP4 / / About 20000
Bacteria resistance
ECP5-1 Ala-Cys-Ser-Ala-Gly 5 510.80 Bacteria resistance
College of Resources and Environmental Sciences, CAUThe methods of earthworm peptide separation and purification have achieved the patent in 2004.
Cyprus conference, 2016
Sequence of amino acid in ECP5-1
College of Resources and Environmental Sciences, CAUAla-Cys-Ser-Ala-Gly
Cyprus conference, 2016Influence of peptide on cancer cells
Th i f EP d EP MGC803 llThe resistance of EP2 and EP3 to MGC803 cell
Scanning electron microsoopical observation on MGC803 Scanning electron microsoopical observation onScanning electron microsoopical observation on MGC803 cells which was untreated with antibacterial peptides
Scanning electron microsoopical observation on MGC803 cells treated with EP2 for 24h
Scanning electron microsoopical observation S i l t i i l b ti
College of Resources and Environmental Sciences, CAU
Scanning electron microsoopical observation on MGC803 cells treated with EP2 for 48h
Scanning electron microsoopical observation on MGC803 cells treated with EP3 for 24h
Cyprus conference, 2016
The character of earthworm antibacterial peptides
●The antibacterial peptide had stable physicochemical properties such as heat stability and wide pH scopeproperties such as heat stability and wide pH scope. There was little influence of pH 3, 5, 7, 9, 11 on the antibacterial activities of antibacterial tetradecapeptideantibacterial activities of antibacterial tetradecapeptide. And the antibacterial tetradecapeptide could keep when storing 180 days in home temperaturestoring 180 days in home temperature.
● The antibacterial mechanism and character of the five earthworm peptides was also researched in our group .
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Influence of Escherichia coli on the antibacterial function from Eisenia fetida
We have a new finding that fat from earthworm have theWe have a new finding that fat from earthworm have the function of bacteria resistance.
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016Vermicasts as function fertilizer
College of Resources and Environmental Sciences, CAUThe resistance of vermicompost to anthracic of cucumber
Cyprus conference, 2016
Two function microbes from vermicompost
0103A (Streptomyces globisporus)
0104A (Streptomyces syringini)( y y g )
Fibrillae of 0103A spores Spores of 0103A Spores of 0104A
Fibrillae of 0104A spores
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Bioreactor for waste conversionCollege of Resources and Environmental Sciences, CAU
Bioreactor for waste conversion A Chinese patent
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Earthworm ProductsCollege of Resources and Environmental Sciences, CAU
Earthworm Products
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Earthworm ProductsCollege of Resources and Environmental Sciences, CAU
Earthworm Products
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Some products developed in CAU
① products processed from earthworm● d f l● amino acid for animal nutrition● foliar fertilizer with amino acid● amino acid biopestcide
② e i o o t② vermicompost:● commercial fertilizers
● soil less culture medium
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
amino acid processing from earthworm
Worm →hydrolyzing with enzyme → raw amino acid→ adding microelement→ raw amino acid → adding microelement
→AA additives → chelating → chelated AA →AA foliar fertilizerg
→AA pesticides
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
amino acid products: biochemical fertilizer
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Cyprus conference, 2016
Micro‐ecological adjustant and function fertilizerfunction fertilizer
① Some effective antagomistic microorganisms g gwere selected from vermicompost (Streptomyces spp) ( p y pp)
②Micro‐ecological adjust agent and soil conditioner plant rhizogenic agentsconditioner plant rhizogenic agents
③ Antibacterial fertilizers and soil regeneration ( Soil‐borne disease of Cucumber seedlings )( Soil‐borne disease of Cucumber seedlings )
④Multi function fertilizers
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Bacterium(0103A) from vermicast
College of Resources and Environmental Sciences, CAUmycelium
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Bacterium(0103A) from vermicast
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E h id dEarthworm peptide and new pesticide‐fertilizernew pesticide‐fertilizer
① 4 peptides extracted from earthworms① 4 peptides extracted from earthworms② effective methods for earthworm peptide
processing (3 patents)③ 3 producing lines in China for the new③ 3 producing lines in China for the new
fertilizer④④ 2 nation certificates for new organic
fertilizer
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
pesticide-fertilizer made of earthworm in China
College of Resources and Environmental Sciences, CAU
pesticide-fertilizer made of earthworm in China
Cyprus conference, 2016
New products and Application of antiNew products and Application of anti‐‐bacterium fertilizers in Chinabacterium fertilizers in Chinabacterium fertilizers in Chinabacterium fertilizers in China
1 Multi1 Multi function biological organic fertilizer:function biological organic fertilizer:1. Multi1. Multi‐‐function biological organic fertilizer:function biological organic fertilizer:Vermicomost and effective microorganismVermicomost and effective microorganism
2 AA f li f ili2 AA f li f ili2. AA foliar fertilizer:2. AA foliar fertilizer:Earthworm and microelementsEarthworm and microelements
3 A ib i l id f ili3 A ib i l id f ili3. Antibacterial peptide fertilizer:3. Antibacterial peptide fertilizer:Biochemistry, earthwormBiochemistry, earthworm
4 V i4 V i4.Vermicompost tea:4.Vermicompost tea:Vermocompost and effective microorganismVermocompost and effective microorganism
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Antiblastic effect of vermicompost on some plant disease
发病率(%)
p
d cd
ab
cd dc
a
70
80
鲜重(0.1g)
d d
b b ba
a
40
50
60
70
株高(0 1 )
株高(0.1cm)
d cdc c
b
c c
b b
c c0
10
20
30
发病率(%)
鲜重(0.1g)
株高(0.1cm)
0CK 10%V 20%V 30%V 50%V 20%V0 健康ck 健康20%V
处理
College of Resources and Environmental Sciences, CAU(V-Vermicompost)
Cyprus conference, 2016
prevention and cure effect on cucumber
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Cyprus conference, 2016
Application on vegetable production
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OOn flowers and ornamentals
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Cyprus conference, 2016
Experiment of Earthworm AA additive on fish
College of Resources and Environmental Sciences, CAU
p
(Qingdao, Shandong, China)
Cyprus conference, 2016Experiment of Earthworm AA additive on pig
College of Resources and Environmental Sciences, CAUCK AA additive
Cyprus conference, 2016Experiment of vermicompost as culture medium on vegetables (Shunyi, Beijing)
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Application of vermicompost on alone
(T h B iji )(Tongzhou, Beijing)
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016Wide foreground of vermiculture and vermiproducts in organic food productiong p
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Earthworm (cast) and products processingBio-organic fertilizer
vermicasts Vermicompost tea
D d i iDeodorization
i ti l
Organicwaste
vermicomposting
earthworms
vermiceuticals
Vermimealearthworms Vermimeal
Amino acids
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016earthworm industrial chain
biomedicinebiomedicinefeedstuff
Health Earthworm
fertilizer foods
cosmetics
College of Resources and Environmental Sciences, CAU
Cyprus conference, 2016
Medicine enzymes and Cosmetic products
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Cyprus conference, 2016
Medicine enzymes from earthworms
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Cyprus conference, 2016
College of Resources and Environmental Sciences, CAUCosmetic products made from earthworm