CYTOLOGICAL, IMMUNOCYTOCHEMICAL AND ULTRAESTRUCTURAL EVALUATION OF

BRONCHOALVEOLAR LAVAGES (BAL) IN PATIENTS WITH PULMONAR HISTIOCITOSIS X .

A STUDY OF THREE CASES.

28th European Congress on Cytology

Antwerpen. Belgium., 2002http://www.garciaureta.com/

CD1a

CD-1a

A 33 year-old man presented with cough.

Respiratory funtion was slighty restrictive.

Slight increase of erythrocyte sedimentation role and leukocytosis.

CASE 1

A 46 year-old man, asymthomatic, that presented leukocytosis and tuberculin skin test positive.

CASE 2

CASE 3

A 45 year-old woman, with 3 months history of nocturnal cough.

Routine hematologic studies were normal.

ANTIBODIES USED

S-100

CD 1a

CD 3

CD 20

CD 68

Ki-67

TOTAL CELL COUNT

• Patient 1: 420.000 cells/ml.

• Patient 2: 380.000 cells/ml.

• Patient 3: 580.000 cells/ml

RESULTS OF BAL

Macrophages Lymphocytes Neutrophils Eosinophils Columnar cells

Patient 1 91 4 2 5 <5%

Patient 2 92 6 1 1 <5%

Patient 3 85 7 6 2 <5%

• Patient 1: 5%

• Patient 2: 7%

• Patient 3: 5%

CD 1a

• Patient 1: 9%

• Patient 2: 8%

• Patient 3: 12%

S-100

• Patient 1: 81%

• Patient 2: 78%

• Patient 3: 75%

CD 68

Ki-67

CD-3

S-100

S-100

CD-1a

FINhttp://www.garciaureta.com/