PhosphorousMetabolismDr.Rohini CSane

Phosphorousmetabolism• Phosphorous :80% present in bones /teeth & 10 % present in muscles • Daily requirement : 5oo mg• Dietary Sources :milk ( 100mg/dl ) cereals nuts moderate sources meat qSerum phosphorous concentration: Adult à 3-4 mg/ dl Children à 5-6 mg/dl Whole blood phosphate : 40 mg/ dl

Serum phosphorous level decreases in Hyperthyroidism, Rickets

Estimation of Serum Inorganic phosphorous by FISKE SUBBRAO METHOD

Estimation of Serum Inorganic phosphorous FISKE SUBBRAO METHOD Serum+TCAàProteinprecipitateFiltrate(De- proteinized serum)+AmmoniumMolybdateà AmmoniumPhosphoMolybdate (HexavalentMolybdenum)AmmoniumPhosphoMolybdate +ANSA(orMETOL)àMolybdenumBLUE

Phosphorousexistsindifferentformsinhumanbody

Freeions

40%

Complexeswithotherions

50%(with

Mg²⁺,K⁺,Na⁺)

Proteinbound

10%

FunctionsofPhosphorous:Ribo-nucleotidesandDeoxy- nuleotide synthesis

FunctionsPhosphorous:integralpartofbiomolecules

MetabolismofPhosphorous

Phosphorousmetabolism

Phosphorousbalanceinhumanbody

HomeostasisofPhosphorousinhumanbody

HomeostasisofPhosphorousinhumanbody

Effectofdietaryphosphatecontenton Phosphorousmetabolism

AcidosisandPhosphateMetabolism