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EXCILONETel. +33(0)1 34 86 77 01 Fax. +33(0)1 34 86 77 53
www.excilone.com
Future is now
PathScan® Combi : High QualityNo Compromise
3rd Digital Pathology Congress EuropeLondon UK
PATHSCAN® Family
Scanning and bright field-fluorescence-FISH Analysis Stations
From the tissue slide…
… to the report
PathScan® Combi : Main features
Fluorescence - FISH
• Nikon motorised Microscope for up to 8 slides
• x2, x4, x10, x20, x40, x60 oil immersion (Plan APO ʎ 1,40 NA)
Turret up to 7 positions
• Adjustable Z-stack / Reconstruction of images
• Motorised holder with up to 5 positions for
fluorescence filter cubes
Bright field and FISH virtual slides
• Sharing platform (optional)
• 12 bits dedicated digital monochrome camera
Resolution: 0.107μm/Pix at x60 oil immersion
Bright field
• LED light source
• Tri CCD colour camera
• Scanning at x10, x20, x40,
x60 oil immersion
Why those technologies?
To meet the technical recommendations from FISH manufacturers (CE-IVD)
To have a quick large field review of the tissues
To be able to do an interpretation at high magnification
To meet the recommandations from the European Cytogeneticists
Association (FISH on histological sections of solid tumors: recommendations from January 2012)
Image quality
Choice of areas of interest (depending on HE or IHC results)
Exclusion of analysis areas
Customised segmentation of nuclei
Results nucleus per nucleus
Hundreds of nuclei per case
Complete archives
Standardized and automatic IA modules
FISH Amplification and Translocation(set by authorised operators)
Nuclei segmentation
Spot identification and spot counting
Ratio calculation (i.e. HER-2/CEP 17) for each
core or in total
Fusion/breakaparts signal detection (i.e. ALK)
Ability to edit images for each core and each spot
Gallery of all cores sorted by score
Summary data table for each field and each core
Z-stack navigation and analysis for each core
User-defined editing of customised reports
Open and programmable IA Modules
Edition of new profile for new probe
Fields number management
Fields size and objectives management
Z-stack management (number and interval)
Fluorochromes management (without number
limitation)
WHY?
Open scanning protocols to be able to
choose probes from different providers
Usage cost control
Customisable IA modules by the operator to
be able to modify algorithms To follow the
evolution of the interpretation rules
Matching Process: Identification from a bright field image
Identifying FOI on the bright field image
Ability to do view/mark up the image remotely
Preview of the FISH slide
and search of the bright field slide in the data base
List of only annotated virtual slides
Case ID number
Matching Process: Identification from a bright field image
Slides are matchedMatching
Navigation (PathScan FISH)
1 field (x60)
1 area (settable)
Objective choice
Summary of slides data
Matching Process: Location from a bright field image
Visualisation: Analysis tools
For Amplification… and Translocation…
The overview indicates the location of the scanned areas
In green the displayed area – In red the other(s) positions of the scanned area(s)
Visualisation : Choice of the scanned area
Visualisation : Z-stack navigation
Integrated data base with multicriteria search
Reports : Customising – total reports or reports per area
French equipped sites since Spring 2014
Thank you!
Questions and answers
