FC Listeria and Salmonella Booklet - Food Safety Summit - FINAL2

Problems with Salmonella and Listeria in Food Products?

Visit us online at foodcheksystems.com

Actero™ Enrichment Media is the SOLUTION!

To purchase our products, contact FoodChek™ distributor 1.800.932.5000 vwr.com

Protecting Your Customers, Their Families and Children from Infection

According to the Centers for Disease Control and Prevention (CDC),

Salmonella causes about 1.4 million illnesses in the United States

every year, with about 23,000 hospitalizations and 450 deaths.

Concerning Listeria, approximately 1600 illnesses and 260 deaths due

to listeriosis occur annually in the United States. These statistics show

the importance for food processors to implement a thorough

pathogen surveillance program to protect human life.

Reduce the Risk of Salmonella or Listeria Contamination in Food Products

The best way to prevent Salmonella infection is to always cook meat

and eggs to the suggested temperatures and be careful not to

contaminate other foods with the juices from uncooked meat, poultry

or eggs. Listeria infection can be prevented by thoroughly cooking all

meats (even lunchmeat, especially if you are pregnant), avoiding raw

or unpasteurized milk, cheese or juice, washing vegetables before

eating and cleaning hands, knives and cutting boards after dealing

with raw meat.

Actero™ Enrichment Media is the Solution for You

Due to sub-lethally injured pathogens cells surviving the

processing treatment of foods, an optimized method of recovery

and enrichment of the bacterium is needed. The Actero™

Enrichment Media provides for effective recovery of injured an/or

dormant pathogens cells from different food and environmental

samples after a single-step enrichment.

The Advantages of Actero™ Enrichment Media

Protect your brand, your customers and their families

Results in an amazing 14 to 30 hours* of enrichment time

Uses less enrichment media per volume compared to standard

enrichment media

Release your products in one day shift*

AOAC validated for a variety of food

Detect using a Real-Time PCR System or by the Actero™ culture

method

* Depending on the food matrix and its complexities

Visit us online at foodcheksystems.com

Foodborne pathogens can be found in

various foods. “Time-to-Results” and

accuracy are critical factors for food

processors being able to provide a safe

food supply, prevent foodborne diseases,

and protecting the brand and customers.

Actero™ Enrichment Media

is the ONLY solution for You

FoodChek’s ActeroTM

Enrichment Media

has received

AOAC PTM CERTIFICATION

on Real-Time PCR Assay and

Lateral Flow System


is the proven solution for problem matrices!

Protect your brand, your customers, their families, and especially their children

Results in an amazing one day shift

Achieve real cost reduction by converting to


foodcheksystems.com | 1.877.298.0208

THE PROBLEM WITH SALMONELLA & LISTERIA

IN FOOD PRODUCTS

Hence, a variety of methods have been developed for rapid detection of foodborne

pathogens as it is required in many food analyses. Most of them require an enrichment step

before being able to apply the detection device.

For all these reasons, FoodChek has developed Actero™ Salmonella Enrichment Media and

Actero™ Listeria Enrichment Media to selectively enrich Salmonella and Listeria in a variety

of foods for its further detection. FoodChek understands the challenging regulatory and

business demands on food producers along with the importance of volume testing and

inventory release that protects your bottom line, without compromising product safety or

the integrity of your brand.

Early and sensitive detection of these foodborne pathogens remains a critical issue in public

health policy. The method to enrich and detect Salmonella and Listeria using Actero™

Enrichment Media was developed with input from the food industry to offer the best

practical

Salmonella spp. (species) is the leading cause of food infection in the United States, with an

estimated number of Salmonella infections of 1.4 million cases per year, and causes the

second largest number of human food-borne illnesses in Europe. Listeriosis caused by

Listeria monocytogenes (L. mono) is low in incidence but its mortality rate is high placing

the microorganism to the top of pathogens contributing to food-borne illness and resulting

in death. Older adults, pregnant women and their fetuses, newborn infants, and other

persons with reduced immune function are particularly at risk for invasive listeriosis.

Salmonella infections have been associated with eating foods, such as meat, eggs and fresh

produce contaminated with animal or human feces. The main causes of Salmonella illness

are poultry and eggs. Listeria monocytogenes has the potential to be present in all raw

foods. Cooked foods can also be contaminated and is usually as the result of post-process

contamination. The pathogen has been isolated from a very wide range of processed foods

including including pâtés, milk, soft cheeses, ice cream, ready-to-eat cooked and fermented meats, smoked and lightly processed fish products and

other seafood.

It is essential to analyze the food for the presence of foodborne pathogens in order to ensure a safe food supply and to minimize the

occurrence of foodborne diseases. The conventional methods used to detect foodborne pathogens are time consuming and laborious.

Hence

With fast, reliable, and easily implemented testing workflows,

FoodChek can help you:

Minimize your risk

Maximize your competitive edge

Reduce your production costs

Protect your brand, your customers and their families

practical solution for a monitoring and surveillance program for these two pathogens.

Despite the inhibitory challenges associated with this sample matrix, our Actero™ Enrichment Media has AOAC-RI CERTIFICATION and is

approved on a variety of food and environmental testing solutions that are designed to accurately screen for Salmonella and Listeria,

with exceptional specificity and speed, helping to reduce costly false positives and inventory holds.


Protecting your brand, your customers; their families and, especially their children,

depend on it.

The FoodChek Team specializes in food microbiology

that helps labs and processors to achieve optimum

efficiency in controlling pathogens throughout the

food processing chain.

The dedicated team of FoodChek professionals

have the expertise in facilitating the adoption of

Actero™ Enrichment Media in your plant by

providing you support in every step of the

conversion process.

As your food safety partner, the FoodChek

team assists you in implementing the best

possible approach for the control and the

monitoring of Salmonella and Listeria in

your food production.

FoodChek understands the importance of

achieving rapid “Time-to-Results” without

compromising accuracy and safety.

The FoodChek Team is well positioned to provide

customized service solutions designed for your specific

requirements.

IT’S EASY TO CONVERT TO

ACTERO™ ENRICHMENT MEDIA


Abstract FoodChek Systems Inc., in collaboration with a Real-Time PCR Assay manufacturer, has developed effective methods for easy and rapid

detection of Salmonella and Listeria in a variety of environmental and food samples. The methods has been successfully granted AOAC

PTM CERTIFICATION. The results of the study demonstrated that performance of the candidate methods is statistically equivalent to the

reference culture method for detecting Salmonella or Listeria in food. The time to detect Salmonella spp. has been reduced up to several

hours, depending the matrix, including the enrichment and detection steps when the Actero™ Salmonella Enrichment Media was used.

Similar observation was obtained for the detection of Listeria in food using Actero™ Listeria Enrichment Media. No false negative or false

positive results were observed. The high accuracy and reliability of the methods were confirmed in the independent laboratory

validation study.

Materials for Salmonella studies Actero™ Salmonella Enrichment Media

Dehydrated format:

• FCM-009 (VWR 97068-832)

• 500 g bottle and three supplement vials

Ready to use liquid formats:

• FCM-047 (VWR 89522-710) – 5 L MediaBox

• FCM-048 (VWR 89522-712) – 10 L MediaBox (with

supplements added)

API 20 E kits for identification of enterobacteriacae

Brilliant Green dye 1% solution

METHOD COMPARISON STUDY REPORT EASY & RAPID DETECTION OF SALMONELLA AND LISTERIA IN FOOD

Real-Time PCR Assay Salmonella, Kit #D14306040*

Hektoen Enteric (HE) agar

Lactose broth

Rappaport-Vassiliadis (RV) broth

Salmonella serogroup E antiserum

Tetrathionate broth (TT)

Trypticase Soy agar plates with 5% of sheep blood agar

Triton X-100

Xylose Lysine Deoxycholate (XLD) agar

* Contact VWR for further details

Materials for Listeria studies Actero™ Listeria Enrichment Media

Dehydrated formats:

• FCM-011 (VWR 97068-830) – 500 g

• FCM-022 (VWR 10158-484) – 2 kg

• FCM-192 (VWR 10146-992) – 1 L MediaPouch

• FCM-193 (VWR 10146-994) – 10 L MediaPouch

Ready to use liquid formats:



Real-Time PCR Assay Genus Listeria Kit #D15131113*

Buffered Listeria enrichment broth (BLEB)

UVM enrichment broth

MOPS – Buffered Listeria enrichment broth (MOPS-BLEB)

Modified Oxoid (MOX) agar plates

Trypticase Soy broth with 0.6% yeast extract (TSA-YE)

Horse blood overlay agar (HL)

Biochemical test panel Listeria API® system


Method for Validation Studies for Detection of Salmonella spp.

METHOD COMPARISON STUDY REPORT EASY & RAPID DETECTION OF SALMONELLA AND LISTERIA IN FOOD

Method for Validation Studies for Detection of Listeria spp. & L. mono

Matrices that are AOAC RI-PTM validated (license number 041403) Matrices Internally Validated by FoodChek Laboratories

Food contact surface – stainless steel and plastic Dry pet food Milk chocolate

Chocolate liquor Cocoa powder Shell eggs

Ground beef Ground chicken Chicken carcass rinse Whole egg powder Wheat flour

Peanut butter Raw almonds Dry raisins Pasteurized liquid egg

Matrices that are AOAC RI-PTM validated (license number 111201) Matrices Internally Validated by FoodChek Laboratories

Food contact surface – stainless steel and plastic No food contact surface – sealed concrete

Hot dog sausage Cold smoked salmon Frozen cooked shrimp Soft Mexican style cheese Fresh bagged spinach

Smoked turkey breast Cured ham Ice cream

Pasteurized milk Raw fish


Results Probability of detection (POD) statistical model was used to evaluate the differences between presumptive and confirmed results as well

as between the alternative and the reference methods.

The results of the different method comparison validation studies for variety of food products are summarized in the table below. POD

analysis didn’t show any significant differences (the 95% confidence interval of the dPODs contains zero) in the method performance

between the Real-Time PCR Assay and the reference methods.

Conclusions The results of the study demonstrated that performance of the Real-Time PCR Assay method to detect Salmonella in a variety of food

samples enriched with Actero™ Salmonella Enrichment Media is equivalent to the reference method. It has been proven that Actero™

Salmonella Enrichment Media is able to selectively enrich Salmonella for its further detection in the shortest possible time.

METHOD COMPARISON STUDY REPORT EASY & RAPID DETECTION OF SALMONELLA IN FOOD PRODUCTS

Matrix CFU/Test Portion

N Real-Time PCR Method

US FDA BAM Chapter 5 Method dPOD

Confirmed POD Confirmed POD

Stainless Steel

0.0 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

50.0 20 12 0.60 12 0.60 0.00 (-0.28; 0.28)

437.0 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)

Plastic

0.0 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

59.4 20 10 0.50 14 0.70 -0.20 (-0.45; 0.10)

400.0 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)

Milk chocolate

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

0.5 20 10 0.50 9 0.45 0.05 (-0.24; 0.33)

8.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)

Chocolate liquor

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

0.1 20 9 0.45 2 0.10 0.35 (0.07; 0.57)

2.7 5 5 1.00 5 1.00 0.00 (-0.28; 0.28)

Cocoa powder

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

0.6 20 10 0.50 9 0.45 0.05 (-0.24; 0.33)

2.0 5 3 0.60 5 1.00 -0.40 (-0.77; 0.12)

Shell egg

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

0.3 20 7 0.35 6 0.30 0.05 (-0.23; 0.32)

1.2 5 4 0.80 4 0.80 0.00 (-0.46; 0.46)

Dry pet food (25 g)

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

1.1 20 13 0.65 16 0.80 -0.15 (-0.40; 0.12)

3.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)

Dry pet food (375 g)

˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)

1.5 20 14 0.70 15 0.75 -0.05 (-0.31; 0.22)

8.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)


Results Probability of detection (POD) statistical model was used to evaluate the differences between presumptive and confirmed results as well

as between the alternative and the reference methods. The validation was done for the detection of Listeria spp. and L. monocytogenes,

however, only the results for the detection of Listeria spp. are shown.

The results of the different method comparison validation studies for variety of food products are summarized in the table below. POD

analysis didn’t show any significant differences (the 95% confidence interval of the dPODs contains zero) in the method performance

between the Real-Time PCR Assay and the reference methods.

Conclusions The results of the study demonstrated that performance of the Real-Time PCR Assay method to detect Listeria in a variety of food

samples enriched with Actero™ Listeria Enrichment Media is equivalent to the reference method. It has been proven that Actero™

Listeria Enrichment Media is able to selectively enrich Listeria for its further detection in the shortest possible time.

METHOD COMPARISON STUDY REPORT EASY & RAPID DETECTION OF LISTERIA IN FOOD PRODUCTS

Matrix CFU/Test Portion

N Real-Time PCR Method

US FDA BAM Chapter 5 Method dPOD

Confirmed POD Confirmed POD

Stainless steel

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

90.0 20 8 0.40 5 0.25 0.15 (-0.13, 0.40)

220.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Plastic

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

4.8 20 10 0.50 5 0.25 0.25 (-0.05, 0.49)

58.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Sealed concrete

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

4.3 20 11 0.55 4 0.20 0.35 (0.05, 0.58)

22.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Frankfurters

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

0.3 20 14 0.70 7 0.35 0.35 (0.04, 0.58)

3.9 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Fresh bagged spinach

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

1.6 20 16 0.80 15 0.75 0.05 (-0.21, 0.30)

1000.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Soft Mexican style cheese

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

0.6 20 10 0.50 8 0.40 0.10 (-0.19, 0.37)

3.6 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Frozen cooked shrimp

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

0.7 20 12 0.60 11 0.55 0.05 (-0.24, 0.33)

5.3 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)

Cold smoked salmon

0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)

1.3 20 14 0.70 14 0.70 0.00 (-0.27, 0.27)

13.9 5 5 1.00 5 1.0 0.00 (-0.43, 0.43)

ACCELERATE YOUR REAL-TIME PCR ASSAY RESULTS WITH ACTERO™ SALMONELLA ENRICHMENT MEDIA

Enrichment of Salmonella spp. in Actero™ Salmonella vs other media prior to Real-Time PCR Assay.

Actero™ Salmonella Enrichment Media Actero™ Salmonella Enrichment Media is a selective media optimized for a rapid single step enrichment of Salmonella from food and environmental samples

FoodChek PN VWR PN Package Size Product Format

FCM-009 97068-836 500g Dehydrated powders and three supplement vials

FCM-047 89522-710 MediaBox™ 5L MediaBox™

Ready to use liquid FCM-048 89522-712 MediaBox™ 10L

To request a sample, please visit vwr.com or call 1.800.932.5000

Matrix ActeroTM Salmonella

Method Media A Method

Validation

Type Size Time Medium volume

Time Medium volume

Stainless steel 100 cm2 14 – 18 h 90 mL 18–24 h 225 mL

AOAC-PTM validated

Plastic 100 cm2 14 – 18 h 90 mL 18–24 h 225 mL

Milk chocolate 25 g 22 – 26 h 175 mL Not validated

Chocolate liquor 25 g 26 – 30 h 225 mL Not validated

Cocoa powder 25 g 16 – 20 h 175 mL 22 – 26 h 225 mL

Dry pet food 25 g 18–22 h 225 mL Not validated

Dry pet food 375 g 18–22 h 2625 mL 22–26 h 3375 mL

Shell eggs 20 eggs 16 – 20 h 1000 mL 48 h 2000 mL

Ground beef 25 g 16 – 20 h 75 mL 20 – 24 h 225 mL

Internally validated

Ground beef 375 g 20 – 24 h 1125 mL 22 – 26 h 1500 mL

Ground chicken 25 g 14 – 18 h 225 mL 16 – 24 h 225 mL

Chicken carcass rinse 1 carcass 16 – 20 h 30 mL 22 – 26 h 30 mL

Whole egg powder 100 g 14 – 18 h 600 mL 22 – 26 h 225 mL

Pasteurized liquid egg 100 g 18 – 22 h 100 mL Not validated

Peanut butter 25 g 16 – 20 h 175 mL 22 – 26 h 225 mL

Raw almonds 375 g 16 – 20 h 750 mL Not validated

Dry raisins 25 g 16 – 20 h 75 mL Not validated

Wheat flour 25 g 18 – 22 h 175 mL Not validated

ACCELERATE YOUR REAL-TIME PCR ASSAY RESULTS WITH ACTERO™ LISTERIA ENRICHMENT MEDIA

Enrichment of Listeria spp. and L. monocytogenes in Actero™ Listeria vs other media prior to Real-Time PCR

Assay.

Matrix ActeroTM Listeria

Method Media A Method

Validation

Type Size Time Medium volume

Time Medium volume

Stainless steel 100 cm2 20 – 24 h 90 mL 24 - 48 h 90 mL

AOAC-PTM validated

Plastic 100 cm2 20 – 24 h 90 mL 18–24 h 90 mL

Sealed concrete 100 cm2 20 – 24 h 90 mL Not validated

Hot dog sausage 125 g 26 – 30 h 750 mL 28 – 48 h 500 mL

Cold smoked salmon 25 g 22 – 24 h 150 mL Not validated

Frozen cooked shrimp 25 g 22 – 24 h 150 mL 24 - 48 h 225mL

Soft Mexican style cheese 25 g 22 – 24 h 150 mL 26 – 48 h 225 mL

Fresh bagged spinach 25 g 22 – 24 h 150 mL 24 – 48 h 225 mL

Smoked turkey breast 125 g 16 – 20 h 500 mL Not validated

Internally validated

Cured ham 125 g 26 – 30 h 500 mL Not validated

Ice cream 25 g 22 – 24 h 150 mL Not validated

Pasteurized milk 25 g (mL) 22 – 24 h 150 mL Not validated

Raw fish 25 g 18 – 22 h 100 mL Not validated

Actero™ Listeria Enrichment Media Actero™ Listeria Enrichment Media is a selective media optimized for a rapid single step enrichment of Listeria from food and environmental samples

FoodChek PN VWR PN Package Size Product Format

FCM-011 97068-830 500 g

Dehydrated powders FCM-022 10146-990 2 kg

FCM-045 89522-706 MediaBox™ 5L MediaBox™

Ready to use liquid FCM-046 89522-708 MediaBox™ 10L

To request a sample, please visit vwr.com or call 1.800.932.5000

FoodChek Systems Inc. 450, 1414 – 8 Street SW, Calgary, Alberta, T2R 1J6, Canada

Terry Macartney Executive Vice President, Operations and Product

Development North America toll free: 1.877.298.0208 x4

Tel: 403.269.9424 x4 Email: [email protected]

Carlos Ruiz Vice President, Strategic Business Development

Cell: 678.648.6121 Email: [email protected]

Carl (Chip) Zerr III

Vice President, Product Integration Tel: 856.839.4213 Cell: 856.200.9184

Email: [email protected]

David Claveau, M.Sc, M.B.A Senior Product Manager

North America toll free: 1.877.298.0208 x2 Tel: 403.269.9424 x2

Email: [email protected]

Problems with Salmonella and Listeria in Food Products? Actero™ Enrichment Media is the SOLUTION!

Science

FC Listeria and Salmonella Booklet - Food Safety Summit - FINAL2