Humanized Antibody Submitted to: Dr. Amber

Submitted by: Mahnoor Khawaja

Definition

◦Humanized antibodies are antibodies made  from non-human species whose protein sequences have been modified to increase their similarity to antibody variants produced naturally in humans.

Era of humanized antibody

Murine

Chimeric

Humanized

Human or Fully Human

Human or fully human

 A human antibody is one of which both chain types, and the J chain in the case of polymeric antibodies, are of human origin.

Preparation Strains used

Xeno mouse

HuMab Mouse

TC Mouse

Technologies used

Phage Display

Transgenic Mice

Primarily human system

Transgenic mice

Phage display

• The genes for the V regions of antibody heavy and light chains can be inserted separately into these phages, and they display the proteins on the surface.

Gene isolated from;

(a) Naïve B-cells

(b) Immunized, antigen-specific memory B-cells.

 

Selection The proteins may be selected by

screening against the antigen of choice, and the desirable proteins may be produced and secreted by bacteria infected by the specific phage.

The secreted proteins can be reassembled in vitro to make functional antibodies. 

Primarily human systemThe best approach so far for producing

antibody . Strategies used ;

Mouse myeloma cell line transfected with human immunoglobulin genes

Fusion of human B cells with a mouse-human hybrid

'heteromyeloma'