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Result
“Some Specificity detected. However due to low antibody concentration result was inconclusive. Peptide test on the antibody is only showing weak signal.“ Q . Fang, Western University
Lane No. Antigen Loading amount Primary antibody Primary antibody dilution ratio
Secondary
antibody dilution ratio
Visualiza-tion time
1 H2BK43me3/2/1
/0 peptide 100 nM
STJ97165 H2BK43me2
antibody
1:1000 1:5000 120S
Materials for Validation: Short histone peptide
Method of validation: Peptide dot blotting
Primary Antibody: STJ97164 H2BK43me2 anti-
body
Secondary Antibody HRP goat anti-rabbit
Reference antibody: Yenzym antibody
Dilution ratio: 1:1000
Protocol
Treatment of materials: 2ul of 100nM peptide was put onto nitrocellulose membrane
Air Dry: 15 mins.
Blocking: 1X TBST with 5% skimmed milk powder for 1 hr at room temperature.
Membrane wash: 10 minutes with TBS-T
Primary antibody incubation: Primary antibody incubated for 2 hours at room temperature.
Membrane wash: 3x 10 minutes with TBS-T.
Secondary antibody incubation: secondary antibody incubated for 1 hour at room temperature.
Membrane wash: 3x 10 minutes with TBS-T
Membrane incubation: Incubate the membrane with ELC reagent for 2 minutes at room temperature.
Antibody Customer Review: STJ97164Anti-H2BK43me2 antibody Antibody Specificity:
Antibody Rating: