PIPETTE

TYPES AND ITS APPLICATIONS

PipettePipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser.

Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes.

Many pipette types work by creating a partial vacuum above the liquid holding chamber and selectively releasing this vacuum to draw up and dispense liquid.

Measurement accuracy varies greatly depending on the style.

History

Eppendorf original, the first piston-stroke pipette.

The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required.

The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started the commercial production of micropipettes in 1961.

The adjustable micropipette is a Wisconsin invention developed through interactions among several people, primarily inventor Warren Gilson and Henry Lardy, a professor of biochemistry at the University of WisconsinMadison.

And it evolved ….

1950Carlsberg Pipettes

1958First Eppendorf Pipette

1974First Gilson Pipette

1984first autoclavable

multichannel pipette.

PresentElectronic

pipette

For details on evolution ,please click here

Types Of PipetteSAir Displacement MicropipettesPositive Displacement PipetteVolumetric PipettePasteur PipetteMohr and Serological Pipettes

Air displacement microPIPETTESThese pipettes operate by piston driven air displacement. A vacuum is generated

by the vertical travel of a metal or ceramic piston within an airtight sleeve.These pipettes are capable of being very precise and accurate.Since they rely on air displacement, they are subject to inaccuracies caused by

the changing environment, particularly temperature and user technique.For these reasons this equipment must be carefully maintained and calibrated,

and users must be trained to exercise correct and consistent technique.

Micropipettes brands include Gilson, ErgoOne, Eppendorf, Hamilton, Rainin, Drummond, BrandTech, Oxford,Hirschmann, Biohit, Labnet, Nichiryo, Socorex, Corning, VistaLab, Thermo, Jencons, Vertex, Handypett, and Pricisexx.

Positive displacement pipette• These are similar to air displacement pipettes, but are less commonly used and are used to avoid

contamination and for volatile or viscous substances at small volumes, such as DNA.• The major difference is that the disposable tip is a microsyringe (plastic), composed of a plunger

which directly displaces the liquid.

volumetric Pipette• Volumetric pipettes or bulb pipette allow the user to measure a volume of

solution extremely precisely (precision of four significant figures).• Typical volumes are 10, 25, and 50 mL. Volumetric pipettes are commonly used

to make laboratory solutions from a base stock as well as prepare solutions for titration.

Pasteur pipette• Pasteur pipettes are plastic or glass pipettes used to transfer small amounts

of liquids, but are not graduated or calibrated for any particular volume.• The bulb is separate from the pipette body.• Pasteur pipettes are also called teat pipettes, droppers, eye droppers and

chemical droppers.

mohr and serological pipette• The graduations on a Mohr Pipette always end before the tip.• Mohr Pipettes are not designed to be completely drained.• The graduation marks on a Serological Pipette always continue till the tip.

MohrPipette

SerologicalPipette

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Volume display is always visible while pipetting

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Explore the XplorerThe electronic pipettes Eppendorf Xplorer and Eppendorf Xplorer Plus were specially designed for high professional standards to provide optimal support for you in your work.

These features have redefined simplicity, precision and reproducibility, which means no more delays due to complicated programming or inflexible processes.

Reduced weight; Improved ergonomics; Perfect balance, Optimally angled pipette head, Improved view & high resolution display; Spring-loaded tip cone provides maximum tightness.

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I always want more

8 Channel 12 channel 16 channel

48 channel96 channel

384 .. 1536 ..List goes on ……

Calibration of PipettesIrrespective of brand or expense of pipette, every micro pipette

manufacturer recommends checking the calibration at least every six months, if used regularly.

Companies in the drug or food industries are required to calibrate their pipettes quarterly (every three months).

Schools which are conducting chemistry classes can have this process annually.

Those studying forensics and research where a great deal of testing is commonplace will perform monthly calibrations.

Tired of calibrating ???Drop a request at sales@isicdelhi.com and we’ll get it calibrated for you ,both standard and premium calibration available.

How to calibrate a pipetteI hear and I forget

I see and I rememberI do and and I understand

Confucius

Do It Yourself

Is my money worth it Pipettes. They can be found in almost every laboratory and, if you’re looking to buy one,

there are many options— manual or electronic, single or multi-channel.Consulting with vendors and their technicians is also helpful. The vendor can supply the

most updated and current replacement parts and probably the lowest cost for that particular product if you go directly to the manufacturer to have it repaired, calibrated or checked.

Despite the up-front cost, buying new means better precision, accuracy, ergonomics, programming, and usability, so users can be more productive and save on reagent costs,

However, if users’ current pipettes aren’t causing any ergonomic injuries, are still accurate, aren’t being used to pipette harsh solutions, and are being maintained and calibrated regularly, there is probably no need to upgrade

Generally, pipettes should be maintained and calibrated on a regular schedule. This will extend the life of the instruments and significantly reduce failure rates.

$ Money Matters $Options for Users with an Unlimited Budget• Buy the latest and greatest technology available• Go for an electronic pipette—the increased accuracy is worth it• Purchase a pipette that uses a positive displacement system, which provides the best precision and

accuracy• If the budget is truly unlimited and the need is there, an automated system is the way to go

Options for Users with a Tight Budget• Ensure pipettes are checked/maintained frequently• Make sure to buy pipettes that are easy for users to maintain and calibrate themselves• Send current pipettes to a service center for a quick check up (can be done for under $20)• Check out the trade-in or lease programs available• Look into replacing several manual pipettes with one electronic one

References

Feel free to drop us an email at sales@isicdelhi.com Or log on to www.isicdelhi.com and know the best offers .We deal in all types of liquid handling/pipetting/lab instruments.

• https://en.wikipedia.org/wiki/Pipette

• http://www.labotec.co.za/articles/different-types-of-laboratory-pipettes/

• http://www.ttelaboratories.com/Accuracy-Matters-Blog/entryid/26/how-to-use-the-various-types-of-pipettes

• http://www.labmanager.com/docs/Pipette_Evolution.pdf