Embed Size (px)
Citation preview
SESSION 2: MORE ACCURATE AND TIMELY DIAGNOSTICS TO BETTER INFORM BIOSECURITY DECISIONS
<title>
Francesco Martoni
Why Taxonomy remains a fundamental weapon for Biosecurity: the case of the New Zealand psyllids.
Francesco MartoniPhD Student – Lincoln University
Plant Biosecurity Cooperative Research Centre
When does a name really matter?
The most important aspect of biosecurity..
..is knowing our (psyllid) Biodiversity!
Part 1:Insects Phylogeny
Part 2:Microflora
The importance of Taxonomy: the science of names.
Psyllid Biodiversity and their names
• At least 99 psyllids • 64 species endemic to New Zealand
• More than 70 different taxa• Including all the families
Genus Trioza (Psylloidea: Triozidae)
Psyl
la ca
rmic
hael
iae
Psyl
la a
pica
lis
a (Big)
B CDE
A
b (small)
• At least 15 new species• A case of synonymy
ID
Analysis of the microbiome of the New Zealand psyllids.
Next Generation Sequencing Illumina MiSeq 16S amplicon protocol
Bioinformatics analysis
Acizzia dodonaeaeAcizzia jucunda Ctenarytaina clavata Ctenarytaina eucalypti
Ctenarytaina fuchsiaCtenarytaina pollicaris
In Conclusion:• The phylogenetic study of the NZ psyllid species revealed at least 15 new
species, different evolutive processes and interesting insect-host plant relationships.
• Patterns in the biodiversity of the microflora can be detected and they are compatible with the insects’ phylogeny.
• This can help in determining if a certain psyllid is more likely to vector a plant pathogen or a bacterium!
• What’s Next?• - Finer scale analysis (e.g. qPCR) of selected S-Symbionts.• - Phylogeny of the P-Symbiont Candidatus Carsonella rudii.
Acknowledgements
Main Supervisor: Karen Armstrong (Lincoln University, New Zealand)
Supervisory team: Simon Bulman (Plant and Food Research, New Zealand) Andrew Pitman (Plant and Food Research, New Zealand) Gary Taylor (The University of Adelaide, Australia)
