Trait observation and discovery network for potato

Hannele Lindqvist-KreuzeRTB Annual Meeting

December 8, 2015

Background problem

● Potato yields of 10-15t/ha in CIP target regions are far lower than the attainable yield

● Process of breeding and release of potato varieties is slow, can take 12-15 years

● Faster genetic gains are required to improve the yields

• Panel of diverse elitepotato lines distributed to NARS

Project purpose

Faster genetic gains

New trait

sources

New methods and tools

Trait data

• Capacity building of NARS researchers

• Trait evaluation protocols

• Data analysis and data management tools

• Large scale on site selection of candidate varieties and trait sources

• Decentralized population improvement through systematic data collection

• User preferences• Observations of other risks and

constraints of local importance

• Baseline trait levels with standard protocols

• Genotyping• GWAS in multiple

target environments

Project Partners

CIP

Max Planck

EIARARARI

YAAS

HAAS

GAU

Funding:• BMZ (2015-2017)• RTB• Partner

complementary projects

Research methodology

Base populations

Standardized protocols

Data analysis tools

Trait evaluations

Genotyping

GWAS

Multi-environment trials

Late blight resistance

Late blight resistance

Late blight resistance

Virus resistance

Virus resistance

Maturity

Maturity

Maturity

Maturity

Maturity

Drought tolerance

Drought tolerance

Drought tolerance

Drought tolerance

Drought tolerance

Workshop on phenomics and genomics for crop improvement

MPI-MP, Germany Nov15th-19th, 2015

Selected Results

Drought trial, Gansu, ChinaAugust 2015

Late blight resistance trial, Yunnan, China July 2015

Yunnan late blight resistance trialHistogram distribution of the sAUDPC values of CIPs advanced potato lines in Yunnan, China, July 2015.

0

10

20

0 5 10SAUDPC

coun

t

Yunnan SAUDPC histogram, Desiree=9

C88 Desiree

Yunnan late blight trialBoxplot of the sAUDPC values of CIPs advanced lines in the different breeding populations.

0.0

2.5

5.0

7.5

10.0

12.5

A B1 B3 B3-HT B3-LTVR BW LTVR LTVR-B3PREBREDVARIETYfactor(population_group)

SA

UD

PC

Yunnan SAUDPC/breeding population

C88

Peru late blight GWASManhattan plot of the marker-phenotype associations for late blight resistance in Peru.

02

46

810

sAUDPC

Chromosome

lo

g 10p

1 2 3 4 5 6 7 8 9 10 12

Gender and variety preference

• Important to understand quality traits preferred by users to allow for more gender responsive targeting

• Building on the RTB work on integrating gender into Participatory Varietal Selection (PVS) for potato in Ethiopia and RTB Database of user preferences

• 2016 test for stakeholder expressed preference using new potato genotypes

Linkages to flagships

FP 1: DISCOVERY: ENHANCED GENETIC RESOURCESBreeding platformNext generation breeding

FP 2: PRODUCTIVE VARIETIES AND QUALITY SEEDPotato varieties for AsiaPotato quality seed

Research team

CIP:Asrat AmeleMerideth BonierbaleGreg ForbesXie KaiyunAwais KhanBritta KowalskiHannele Lindqvist-KreuzeElisa MihovilovichGedif MulugetaElisa SalasJunhong Quin

Partners:Karin Koehl (Max Planck)Xianping Li (YAAS)Abebe Chindi (EIAR)Ebrahim Seid (EIAR)Alemu Worku (ARARI)Zhang Junlian (GAU)Sheng Wangmin(HAAS)