BVisionBVision Dedicated for the Morris water maze test study of effect of different compounds on...

BVisionDedicated for the

Morris water maze test

study of effect of different compounds on spatial orientation and

memory of experimental animals

Video camera

Water poolHidden Platform

Animal (rat)

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Data

Tracking System Analyzing Tools

Acquisition

Management

Visualization

Analysis

Configuration parameters

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Automatic creation of the video archive

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• Program correction of the video path in the framegrabber.

• Minimizing load of the processor.

• Fast signal proceeding (up to 25 frames/s

• Displaying zones boundaries

Live Video

Data structure and storage Flexible database management system.

Tracks and Experiments Stored in the Database

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Arena and zones definition Automatical detection of borders of the pool and other

analyzed zones (for example, platform)

Result of Zones Detection

Restoration of the Platform Position

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Object Detection Algorithm

Формальный нейрон

Framegrabber Input video signal

Program correction of the

video path

Differentiation of all the found

objects by size

Corrected video signal

Detected animal

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Differentiation of the residual objects

according to physical reasons

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Tracking process

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Parameters

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• escape latency,

• accounting for time of active swimming only (with speed lower than selected threshold),

• trajectory length,

• speed,

• index of “pasive” swimming” (to characte-rize decrease of animals’ mobility),

• time in zone of interest (ZOI),

• % of tracks failed to find the platform,

• straightness index (deviation from the direct path to platform),

• cumulative and reverse cumulative distances to platform,

• angle characteristics of movement relative platform,

• Whishaw “alley” index, etc.

Analysis of a Single Track

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Zones Definitions for Analysis

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Main Analysis Window

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Results Visualization

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