FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND … · FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY...

F R A U N H O F E R I N S T I T U T E F O R M O L E C U L A R B I O L O G Y A N D A P P L I E D E C O L O G Y I M E

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IN VITRO TESTS1 Multi-well plate.

2 Macrophages in lesions of EAE

mice.

A wide variety of in vitro tests are available

within the Fraunhofer IME Project Group

Translational Medicine and Pharmacology.

Emphasis is laid on cellular signaling

processes, lipid metabolism, neuronal

cell responses and immune cell activity.

Cytotoxicity, mutagenicity, proliferation

and apoptosis assays are performed.

State-of-the art cellular, immunological,

biochemical, analytical, proteomic and

genomic techniques are employed.

Cellular models for receptor-binding

• G-protein-linked

• eicosanoid receptor binding

Cellular lipid metabolism

• sphingolipid metabolism, sphingosine

kinases, S1P lyase

• sphingosine -1-P receptor binding and

signaling

• eicosanoid metabolism

• endocannabinoid metabolism

Signaling assays

• calcium imaging of dorsal root ganglia

and spinal cord neurons

• chip assay

• miRNA analysis

Imaging

• Multi-Epitope-Ligand-Cartography

• immunofluorescence assays

Gene transfection

• lentiviral gene transfer

• in-situ hybridization

• cloning

• promoter-reporter gene assay

Single and co-cultures of immune

competent cells

• lymphocytes

• macrophages

• dendritic cells

• keratinocytes

• microglia

• synovial cells

• cytokine release and signalling

Other cell types

platelets,fibroblasts, renal mesenchymal

cells, podocytes, tumor cell lines, astrocytes,

neuroblastoma cell lines, primary neuronal

cultures

Fraunhofer Institute for

Molecular Biology and Applied

Ecology IME

Project Group for Translational

Medicine and Pharmacology TMP

Theodor-Stern-Kai 7

60596 Frankfurt am Main

Contact

Prof. Dr. Michael Parnham

Phone +49 69 6301-84234

michael.parnham@ime.fraunhofer.de

www.ime.fraunhofer.de

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