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8/7/2019 Web Snap Bean Virus
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Status of VirusStatus of Virusand Virusand Virus--likelike
Problems onProblems on
Processing BeansProcessing Beansin Wisconsin During 2000in Wisconsin During 2000
SYAIFUL BAKHRI
BIOLOGY ICP 08UNIVERSITAS NEGERI
MAKASSAR
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Description of FieldDescription of FieldSituation in 2000Situation in 2000
Problematic fields near Belgium and Cambria,Problematic fields near Belgium and Cambria,
WisconsinWisconsin
High incidence of plants with virusHigh incidence of plants with virus--likelike
symptomssymptoms
Randomly distributed symptoms in the fieldRandomly distributed symptoms in the field
Many fields exhibiting these symptoms wereMany fields exhibiting these symptoms weredestroyed prior to harvestdestroyed prior to harvest
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Description of FieldDescription of FieldSituation in 2000Situation in 2000
Symptoms observed in the field:Symptoms observed in the field:
Pod distortionPod distortion
Affected pods had a dark brown discolorationAffected pods had a dark brown discoloration
internallyinternally
Leaf distortionLeaf distortion Mottling and mosaic patternsMottling and mosaic patterns
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Symptoms observed at CambriaSymptoms observed at Cambria
Leaf mottling and mosaicLeaf mottling and mosaic
Internal pod discolorationInternal pod discoloration
Pod necrosisPod necrosis
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Leaf mosaic and
necrosis, Cambria
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Leaf mosaicat Cambria
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Rugosity at
Cambria
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Leaf mosaic at
Cambria
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Pod necrosis at
Cambria
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Deformed pods
from Cambria
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Symptoms observed at BelgiumSymptoms observed at Belgium
Leaf mottling and mosaicLeaf mottling and mosaic
Internal pod discolorationInternal pod discoloration
Pod necrosisPod necrosis
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Mottling on leaves
at Belgium
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Leaf mottling at
Belgium
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Rugosity and
mottling at Belgium
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Leaf rugosity
at Belgium
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Pod necrosis
at Belgium
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Pod necrosis at Belgium
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Virus Assays Conducted forVirus Assays Conducted for
Belgium samplesBelgium samples Soybean mosaic virusSoybean mosaic virus
(SMV)(SMV)
Alfalfa mosaic virusAlfalfa mosaic virus(AMV)(AMV)
Clover yellow vein virusClover yellow vein virus
(CYVV)(CYVV)--conducted by Dr.conducted by Dr.
Robert Gilbertson, Univ. of CARobert Gilbertson, Univ. of CA--DavisDavis
Bean yellow mosaicBean yellow mosaic
(BYMV)(BYMV)
Tobacco streak virusTobacco streak virus(TSV)(TSV)
Bean common mosaicBean common mosaic
virus (BCMV)virus (BCMV)
Bean pod mottle virusBean pod mottle virus(BPMV)(BPMV)
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Virus Assays Conducted forVirus Assays Conducted for
Cambria samplesCambria samples Alfalfa mosaic virusAlfalfa mosaic virus
(AMV)(AMV)
Bean yellow mosaic virusBean yellow mosaic virus
(BYMV)(BYMV)
Tobacco streak virusTobacco streak virus
(TSV)(TSV)
Node discoloration atNode discoloration at
CambriaCambria
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Viruses Detected in SymptomaticViruses Detected in Symptomatic
Snap BeansSnap Beans
Viruses AssayedViruses Assayed
AMVAMV BCMVBCMV BYVVBYVV CYVVCYVV TSVTSV
% samples positive% samples positive
BelgiumBelgium 8585 1515 88 100100 1515
CambriaCambria 6767 NANA 00 NANA 00
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Greenhouse ExperimentsGreenhouse Experiments
Inoculum from field infected snap beans causedInoculum from field infected snap beans causedsimilar symptoms on inoculated plantssimilar symptoms on inoculated plants
Pods provided the best source for virus detectionPods provided the best source for virus detectionand transmissionand transmission
Symptoms expressed confirmed by ELISA assaySymptoms expressed confirmed by ELISA assayfor AMVfor AMV
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Transmission of StyletTransmission of Stylet--Borne SnapBorne Snap
Bean VirusesBean Viruses
Aphid transmitted
Alfalfa mosaicAlfalfa mosaicvirus (AMV)virus (AMV)
Aphid and seed transmittedAphid and seed transmitted
Clover yellow veinClover yellow vein
virus (CYVV)virus (CYVV)
Bean yellow mosaicBean yellow mosaicvirus (BYMV)virus (BYMV)
Aphid transmittedAphid transmitted
Center for IPM; NC State
Center for IPM; NC State
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Transmission of StyletTransmission of Stylet--Borne SnapBorne Snap
Bean VirusesBean Viruses
Bean commonBean common
mosaic virusmosaic virus
(BCMV)(BCMV)
Tobacco streak virusTobacco streak virus
(TSV)(TSV)Thrips transmittedThrips transmitted
Aphid transmittedAphid transmitted
Center for IPM; NC State
http://newcrop.hort.purdue.edu/rhodcv/
hort410/peas/beanfl.jpg
Seed and pollenSeed and pollen
transmittedtransmitted
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Snap Bean Virus InoculumSnap Bean Virus Inoculum
Sources and Insect TransmissionSources and Insect Transmission
AphidAphid
OtherOther
LegumesLegumes
SeedSeed
AMV, BCMV,AMV, BCMV,
CYVV, BYMVCYVV, BYMV
AMV, BCMV, TSVAMV, BCMV, TSV
AMV, CYVVAMV, CYVV
BYMV, TSVBYMV, TSV
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Typical Symptoms for Detected VirusesTypical Symptoms for Detected Viruses
VirusVirus Leaf Leaf NecrosisNecrosis PodPod
AMVAMV DeformedDeformed MildMild MalformedMalformed
CYVVCYVV Yellow mosaicYellow mosaic ApicalApical Severe malformation; wallSevere malformation; wall
discoloration?discoloration?
BYMVBYMV Yellow mosaicYellow mosaic ApicalApical Slight malformationSlight malformation
BCMVBCMV Green mosaicGreen mosaic SevereSevere Wall discolorationWall discoloration
TSVTSV Dark veinsDark veins Red nodesRed nodes Malformed , lesionsMalformed , lesions
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ConclusionsConclusions Alfalfa mosaic virus (AMV), clover yellow vein (CYVV),Alfalfa mosaic virus (AMV), clover yellow vein (CYVV),
bean common mosaic virus (BCMV), and tobacco streak virusbean common mosaic virus (BCMV), and tobacco streak virus
(TSV) are associated with symptoms observed in 2000.(TSV) are associated with symptoms observed in 2000.
Although AMV was commonly detected, field symptoms wereAlthough AMV was commonly detected, field symptoms weremore closely associated with symptoms characteristic of clovermore closely associated with symptoms characteristic of clover
yellow vein (CYVV) and bean common mosaic virusyellow vein (CYVV) and bean common mosaic virus
(BCMV).(BCMV).
An aphid transmitted complex of viruses are present and meritAn aphid transmitted complex of viruses are present and meritfurther investigation.further investigation.
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Proposed Further ResearchProposed Further Research
Field survey of snap beans and other legumes forField survey of snap beans and other legumes for
types and frequency of viruses.types and frequency of viruses.
Determine whether viruses in snap beans areDetermine whether viruses in snap beans are
common to other legumes.common to other legumes.
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Proposed Further ResearchProposed Further Research
Evaluate snap beans cultivars and germplasm forEvaluate snap beans cultivars and germplasm for
reaction to AMV and CYVV.reaction to AMV and CYVV.
Determine seed transmission ofDetermine seed transmission of
detected viruses.detected viruses.
Determine if viruses are a component of theDetermine if viruses are a component of the
Disease X symptoms in snap beans.Disease X symptoms in snap beans.
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