Histology Portfolio

Histology Portfolio

Flavia Nodarse MartinezBIO 230-01 Histology

Prof. Abdallah M. Matari

Content The cellMitosisEpithelial tissue Connective tissue

Blood smearsCartilage and bone

Muscle tissue Nervous tissue

The Cell

Elodea leaf w.m. Chloroplasts

40x 100xCell wall Cytoplasm

Giant Chromosomes

100xChromosome

400xCytoplasm Centromere

Dorsal Root ganglion Golgi apparatus sec. silverstain

100x 400xNucleus

Golgi apparatus

Nucleic Acid onion root tip Azure B Stain sec.

100x 400xNucleus Cell wallCytoplasm

Nucleolus

DNA in animal cells sec. Feulgen

100x 400xNucleus (N), nucleolus (n), mitochondria (M), plasma membrane (Pm), cytoplasm (C)

N

nM

C

Pm

Epidermal cells of Allium cepa (onion)

40xCell wall (cw), cytoplasm (c), nucleus (N)

100x

cw

c

N

N

Mitosis

Crayfish testis mitosis/meiosis IH sec.

100x

P – prophaseM – metaphaseA – anaphaseT – telophase

A➝ P➝

M➝

T➝

Crayfish testis mitosis/meiosis IH sec.

P – prophaseM – metaphaseA – anaphaseT – telophaseC – cytokinesis

400x

A➝

P➝

M➝

T➝ C➝

Mitosis Onion root tip IH l.s.

100x

P – prophaseM – metaphaseA – anaphaseT – telophase

M➝P➝

A➝ T➝

Mitosis Onion root tip IH l.s.

400x

P – prophaseM – metaphaseA – anaphaseT – telophase

P➝

A➝

M➝ T➝

Epithelial Tissue

Epithelium Squamous section

100x 400xSsSimple squamous epithelium (Ss), Lumen (L), Nuclei (N) LN

L

Epithelium Cuboidal section

100x 400xSimple cuboidal epithelium (Scu), Lumen (L), Nuclei (N)

L

Scu NScu

Epithelium Pseudostratified Columnar

(ciliated) section

100x 400xCilia (C), Pseudostratified columnar epithelium (PsC), Nuclei (N), Goblet cells (GC), Basal cells (BC)

GC

C

NPsC

BC

Epithelium simple columnar section

100x 400xSimple columnar epithelium (Sc), Nuclei (N), Lumen (L), Basement membrane (Bm), Goblet cell (GC)

Sc N

LBm

GC

Epithelium Stratified Squamous non-cornified

40x 100xStratified squamous non-keratinized epithelium (StS), Basal layer (BL), Lumen (L), Nuclei (N)

StS

BL

L

N

Epithelium Transitional section

100x

400xTransitional epithelium (T), Round apical cells (Ra), Lumen (L), Nuclei (N), Basement membrane (BM)

T

L

Ra

N

BM

Olfactory epithelium mammal sec. H&E

100x 400xPseudostratified columnar epithelium (PsC), Cilia (C), Goblet cell (GC), Basement membrane (BM), Connective tissue (CT), Nuclei (N), Lumen (L)

CT

L

N

CGCPsC

BM

Trachea ciliated epithelium c.s. H&E

100x 400xSimple columnar epithelium (SC), Cilia (C), Nuclei (N), Basement membrane (BM), Connective tissue (CT),Lumen (L)

SC

L

SC

C

N

BM

CT

Connective Tissue

Adipose tissue fat (lipid) stained, w.m.

40x 100xAdipocyte

Lipid inclusionAdipose tissue

Nuclei

Adipose tissue fat (lipid) stained, w.m.

40x 100xNuclei Adipocyte

Lipid inclusion

Areolar Tissue

100x 400xFibroblast

Elastic fibers

Collagen fibers

Ligament yellow elastic connective tissue c.s. & l.s. H&E

100x 400x

Elastic fibers

Palatine tonsil human sec. H&E

100x 400x

Palatine epithelium(stratified squamous epithelium)

Connective tissue

Reticular tissue sec. m&nfr

100x 400x

Reticular fibers

Tendon White fibrous connective tissue l.s.

100x 400x

Dense regular tissue Fibroblasts

Collagen fibers

Fibroblast nuclei

White Fibrous Tissue; Tendon (Human), l.s.

100x 400x

Fibroblast

Collagen fibers

Dense regular connective tissue

Blood Smears

Bird blood smear

400x 1000x

Nucleatederythrocytes

Leukocytes

Frog blood smear

100x 400x

Nucleatederythrocytes

Leukocytes

Nuclei

Reptile blood

100x 400x

Nucleated erythrocytes

Thrombocytes

Human blood smear WrightErythrocytes

100x 400xLymphocyte

MonocyteLeukocytes

Human blood smear Wright (continuation)

Platelet

400x

Neutrophil

Monocyte400x

Human blood smear Wright (continuation)

Basophil

Eosinophil400x 400x

Red bone marrow mammal section H&E

100x 400x

AdipocytesBone marrow

Haversian canal Osteocyte

EndosteumMegakaryocytes

Cartilage & Bone

Hyaline cartilage trachea H&E sec.

100x 400x

Isogenous group

Chondrocytes embeddedin lacunae

Fibrous & chondrogenic layersPerichondrium

Matrix

Hyaline cartilage xiphisternum H&E sec.

Isogenous group

100x 400xMatrix

Perichondrium containing chondroblasts Chondrocytes

Elastic cartilage epiglottis sec.

100x 400x

Perichondrium

Chondrocytes Elastic fibers

White fibro-cartilage intervertebral disc sec.

100x 400x

Collagen fibers

Chondrocytes

Bone decalcified l.s. H&E

100x 400xOsteocytes

Volkmann’s canal Periosteum

Bone dry ground human c.s.

100x 400xCanaliculi

LamellaeHaversian canal

Osteocytes

Osteons

Compact bone decalcified H&E c.s.

100x 400xVolkman’s canalHaversian canal

Osteon

Intramembranous ossification fetal skull sec.

40x 100xCalcified bone Ossification center

Osteocytes Osteoblasts

Osteogenesis endochondral ossification

l.s. mal

40x 100xTrabeculae

Bone marrow Epiphysis

Periosteum Proliferation zone

Hypertrophic zoneCalcification zone

Ossification zone

Muscle Tissue

Cardiac muscle sec. H&E

100x 400x

Cardiac muscletransverse section

Myofiber

Connective tissueNuclei

Skeletal muscle human c.s. & l.s.

100x 400xI band (light) A band (dark)Sarcomeres

Skeletal muscle fibers (longitudinal section)

Nuclei

Skeletal muscle l.s. IH

100x 400xI band (light) A band (dark)

SarcomereSkeletal muscle fiber (longitudinal section)

Skeletal muscle tongue c.s. & l.s. fibers H&E

100x 400xNuclei I band

(light)A band(dark)

SarcomereSkeletal muscle fibers (longitudinal section)Skeletal muscle fibers

(cross section)

Smooth muscle intestine c.s. & l.s.

100x

400x

Smooth muscle fibers(cross section)

Smooth muscle fibers(longitudinal section)

Nuclei

Striated Muscle Mammalian Tongue c.s.

mas

100x 400x

Skeletal muscle fibers(cross section)

Skeletal muscle fibers (longitudinal section)

Nuclei Sarcomere

Nervous Tissue

Cerebellum & choroid plexus sec. H&E

40x 100x

White matter

Grey matter

Purkinje cell layer

Molecular layerGranule celllayer

Pia matter

Cerebellum anti-neurofilament

cytoskeleton sec.

40x 100xWhite matter

Grey matterPia matter

Purkinje cell layer

Molecular layer

Cerebrum mammal sec. CV

40x 400xPia matter White matter

Grey matter

Neurons

Neuroglia

Motor nerve endings reptile w.m.

40x 100xMotor end plates Axon terminals

Myelinated nerveAxon

Skeletal muscle fibers

Neurofibrillae Nerve cells sec. Spinal cord

100x 400xNeurophil

Multipolar neurons

Neuroglia cells & fibers sec.

100x 400xNeuroglial cells

Neurons

Peripheral nerve myelin Osmic Acid

100x 400x Myelinated axon

Perineurium

Axons

Endoneurium

Retina mammal sec. H&E

100x

400x

Ganglion cells

1

2

3

(1) Nerve fibers to optic nerve, (2)Bipolar cells, (3) Rod and cone nuclei, (4) Rod and cone outer segments, (5) Pigment epithelium, (6) Choroid, (7) Sclera

4

56 7

Spinal cord central nervous tissue c.s. H&E

40x 100xWhite matter

Grey matterCentral canal

Ventral horn

Spinal cord multipolar motor neurons nerve tissue smear MB&P

AxonAxon hillock

Multipolar neuron

Cell body

DendritesNeuroglial cells

Nucleus 400x100x

Sympathetic ganglion sec. H&E

100x 400xNeurons

Satellite cells surrounding the neurons

Axons Schwann cells

Referenceshttps://micro.magnet.fsu.edu/optics/intelplay/

gallery/pages/brightfield/femurmammal.htmlhttp://legacy.owensboro.kctcs.edu/gcaplan/

anat/Notes/API%20Notes%20F%20Connective%20Tissues.htm

http://www.histology-world.com/photoalbum/displayimage.php?album=97&pid=1099

https://www.studyblue.com/notes/note/n/neurobiology-exam-i/deck/5218020