EN - LOREENA User Manual - Rev1.3.pdf - AFI Centrifuges

User Manual - Revision 1.3 - English Version

601997

This document has been prepared with the utmost care. However, AFI Centrifuge refuses to accept any responsibility in the event of errors or omissions. The same applies to any damage resulting from the use of information contained in this manual.

AFI Centrifuge 3, Rue Nicolas Copernic ZA Nord Bazouges 53200 CHATEAU-GONTIER FRANCE +33 (0) 2 43 06 66 76

Originale Instructions- Rev.1.3 User Maunal– Loreena 1/ 68

LOREENA CENTRIFUGE

Models

AFI-C300V

AFI –C300R

AFI-C300RF

AFI –C300V-E

AFI –C300R-E

AFI-C300RF-E

Operation Manual

(115 VAC, 60 Hz) (230 VAC, 50 Hz)

Manufactured by:

AFI Centrifuge Rue N. Copernic

ZI Nord Bazouges 53200 CHATEAU-GONTIER

FRANCE

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Revision modifications

Date Revision Modifications Paragraph Author August, 2018 1.3 End of cycle acquitment, Precool & Warmup

new shortcuts, "routine" program, End of cycle Flashing signal, information menu modifications; Add new rotors & rotor specifications modifications.

4.6, 4.7, 4.9, 4.10, 4.11, 0, 4.14, 9

F. LE CAM


You have just purchased one of the finest Laboratory Centrifuges available. With proper care, maintenance and laboratory procedure, this centrifuge will provide you years of productive service. Please read this manual carefully to familiarize you with proper installation, and operation of the centrifuge. Other information are available on our website,

www.aficentrifuge.com .

Warranty AFI Centrifuge. warrants AFI-C200 Series LISA Centrifuge that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be directed to AFI Centrifuge. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment. This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, or flood. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety. The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any item, for any reason, contact AFI Centrifuge for a Return Authorization Number. This number must accompany all returns. Any product shipped to AFI Centrifuge without this number will be returned refused shipment or collect freight. Explanation of Symbols

Safety alert symbol indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury

CAUTION used without the safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in property damage.

Safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury.

Pinching: This warning symbol indicates the presence of a risk of pinching when handling the lid.

Note: Used for important information

Potential electrical hazard, only qualified person to access

Biohazard

Ground, Earth

Lead Free

WARNING

CAUTION

CAUTION

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Table of Contents

1. GENERAL INFORMATION ------------------------------------------------------------------------------------------------------ 6

1.1. DESCRIPTION ....................................................................................................................................................... 6 1.2. INTENDED USE ..................................................................................................................................................... 6 1.3. PROHIBITIONS: .................................................................................................................................................... 7 1.4. MODELS, SPECIFICATIONS AND ACCESSORIES ............................................................................................................. 8 1.4.1. MODELS & SPECIFICATIONS ................................................................................................................................... 8 1.4.2. ACCESSORIES ....................................................................................................................................................... 9 1.5. SAFETY INSTRUCTIONS ......................................................................................................................................... 12 1.6. INFORMATION ON THE REFRIGERANT FLUID USED ...................................................................................................... 12

2. INSTALLATION INSTRUCTIONS ---------------------------------------------------------------------------------------------- 13

2.1. SHIPMENT ........................................................................................................................................................ 13 2.1.1. DAMAGED SHIPMENTS ........................................................................................................................................ 13 2.1.2. HANDLING AND TRANSPORT ................................................................................................................................. 13 2.2. SITE PREPARATION .............................................................................................................................................. 13 2.3. ELECTRICAL ENVIRONMENT .................................................................................................................................. 14 2.4. UNPACKING ...................................................................................................................................................... 14 2.5. PACKING LIST ..................................................................................................................................................... 14 2.6. DESCRIPTION ..................................................................................................................................................... 15

3. OPERATING THE LOREENA CENTRIFUGE - AFI-C300 ------------------------------------------------------------------- 16

3.1. FIRST START / POWERING .................................................................................................................................... 16 3.2. LEVELLING (FLOOR MODEL) .................................................................................................................................. 17 3.3. ROTOR ASSEMBLY ............................................................................................................................................... 17 3.4. POSITION THE BUCKETS ....................................................................................................................................... 17 3.5. FIRST POWERING ................................................................................................................................................ 17 3.6. USING THE INTERFACE ......................................................................................................................................... 18 3.7. OPENING THE LID ............................................................................................................................................... 18 3.8. ROTOR LOADING AND BALANCING ......................................................................................................................... 18 3.9. QUICK START-UP ................................................................................................................................................ 19

4. OPERATING ------------------------------------------------------------------------------------------------------------------ 20

4.1. SETTINGS .......................................................................................................................................................... 20 4.2. CREATE / EDIT A PROGRAM NAME ......................................................................................................................... 20 4.3. SET PROGRAM SETTINGS ...................................................................................................................................... 21 4.4. SET SPEED IN RPM OR RCF ................................................................................................................................. 22 4.5. PROTECT THE SETTINGS OF A PROGRAM .................................................................................................................. 23 4.6. CENTRIFUGE: USE A PROGRAM ............................................................................................................................. 23 4.7. INTERRUPT CENTRIFUGATION ................................................................................................................................ 24 4.8. OPEN AFTER AN ELECTRIC POWER FAILURE / EMERGENCY STOP ................................................................................... 24 4.9. PRECOOLING ..................................................................................................................................................... 25 4.10. PREWARMING ................................................................................................................................................... 25 4.11. ROUTINE MENU................................................................................................................................................. 26 4.12. OTHER MENUS ................................................................................................................................................... 26 4.12.1. USER PREFERENCES ............................................................................................................................................ 28 4.12.2. TYPE OF SPEED DISPLAYED (RPM OR RCF) ............................................................................................................. 29 4.12.3. FREQUENCY OF LUBRICATION ................................................................................................................................ 30 4.12.4. ACCESS TO LOGBOOK .......................................................................................................................................... 30 4.12.5. ACCESS TO ERROR LOGBOOK ................................................................................................................................. 30 4.12.6. TYPE OF TEMPERATURE DISPLAYED ........................................................................................................................ 31 4.12.7. TIMER MODE ..................................................................................................................................................... 31 4.12.8. COUNTING MODE ............................................................................................................................................... 32 4.12.9. KEYBOARD TYPE ................................................................................................................................................. 32 4.12.10. CHANGE BACKGROUND COLOR .............................................................................................................................. 33 4.12.11. END OF CYCLE ALARM ......................................................................................................................................... 33 4.12.12. ERROR MESSAGE ALARM ..................................................................................................................................... 33 4.12.13. AUTOMATIC LID OPENING .................................................................................................................................... 33 4.12.14. SLEEP MODE ...................................................................................................................................................... 34 4.12.15. POST COOL ....................................................................................................................................................... 34 4.12.16. PRE COOL FUNCTION .......................................................................................................................................... 35 4.12.17. TEMPERATURE DISPLAY MODE ............................................................................................................................. 35


4.13. SERVICE MENU .................................................................................................................................................. 36 4.14. INFORMATION ................................................................................................................................................... 36 4.15. ENERGY SAVING ................................................................................................................................................. 36

5. CARE AND MAINTENANCE -------------------------------------------------------------------------------------------------- 37

5.1. CARE AND CLEANING .......................................................................................................................................... 37 5.1.1. AUTHORIZED MAINTENANCE PRODUCTS .................................................................................................................. 37 5.1.2. CENTRIFUGE ...................................................................................................................................................... 37 5.1.3. ROTOR & ACCESSORIES ....................................................................................................................................... 38 5.1.4. DISINFECTION / DECONTAMINATION ...................................................................................................................... 38 5.2. DISMANTLING THE ROTOR .................................................................................................................................... 39 5.3. LIMITING THE DURATION OF OPERATION ................................................................................................................. 39 5.4. CHECKING AND TESTING ACCESSORIES .................................................................................................................... 40 5.5. STORAGE OF ACCESSORIES .................................................................................................................................... 40 5.6. ANNUAL MAINTENANCE....................................................................................................................................... 40 5.7. UNBALANCING SENSITIVITY DETECTION ................................................................................................................... 41 5.8. ERROR MESSAGES, TROUBLESHOOTING, OPTION-DIAGNOSTICS .................................................................................. 42 5.9. WEARING PARTS & CONSUMMABLE ....................................................................................................................... 45 5.10. BACK TO MANUFACTURER .................................................................................................................................... 45

6. ELECTRICAL/ENVIRONMENTAL REQUIREMENTS -------------------------------------------------------------------------- 46

6.1. ELECTRICAL (SUPPLY VOLTAGE FLUCTUATIONS NOT TO EXCEED +/- 10%) .................................................................... 46 6.2. OPERATIONAL PERFORMANCE (FOR INDOOR USE ONLY) ............................................................................................. 46 6.3. LIGHT EXPOSURE ................................................................................................................................................ 46 6.4. INSTALLATION CATEGORY: 2.0 .............................................................................................................................. 46 6.5. POLLUTION DEGREE: 2.0 .................................................................................................................................... 46 6.6. CHEMICAL EXPOSURE .......................................................................................................................................... 46 6.7. EMC PERFORMANCE (CLASSIFIED FOR LIGHT INDUSTRIAL) .......................................................................................... 46

7. DISPOSAL AND RECYCLE ----------------------------------------------------------------------------------------------------- 47

8. THEORETICAL BASICS OF CENTRIFUGATION ------------------------------------------------------------------------------- 48

8.1. BASICS ............................................................................................................................................................. 48 8.2. NOMOGRAM ..................................................................................................................................................... 49 8.3. LOGBOOK ......................................................................................................................................................... 50 8.4. RETURN COUPON ............................................................................................................................................... 51

9. ANNEX 1: ACCESSORY SPECIFICATIONS ------------------------------------------------------------------------------------ 52

9.1. SWING-OUT ROTOR NU-RX1000 ........................................................................................................................ 52 9.2. SWING-OUT ROTOR NU-RX750 .......................................................................................................................... 55 9.3. SWING-OUT ROTOR NU-RHDW .......................................................................................................................... 58 9.4. ANGULAR ROTOR AFI-RHD3 ............................................................................................................................... 59 9.5. ANGLE ROTOR NU-RA8-50 ................................................................................................................................ 62 9.6. ANGLE ROTOR NU-RA30-15 .............................................................................................................................. 63 9.7. ANGLE ROTOR NU-RA16-5 ................................................................................................................................ 64 9.8. ANGLE ROTOR NU-RA48-2 ................................................................................................................................ 65 9.9. ANGLE ROTOR NU-RA30-2 ................................................................................................................................ 66

10. EC CONFORMITY DECLARATION -------------------------------------------------------------------------------------------- 67


LOREENA CENTRIFUGE

1. General Information

1.1. Description The LOREENA Centrifuge is a laboratory series of centrifuges that maximizes productivity while minimizing laboratory required bench space. The centrifuge functionality allows the separation of substances of different densities held in suspension or emulsion in a liquid using centrifugal force. The LOREENA Centrifuge is available in two models, one ventilated and the other Refrigerating. The LOREENA Centrifuge ventilated model has a unique ventilation system to maximize performance within laboratory ambient temperature conditions. The LOREENA Centrifuge Refrigerating model adds precise temperature control to further maximize performance. The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.

Benchtop ventilated model Benchtop Refrigerating model Floor Refrigerating Model

LOREENA Centrifuges

1.2. Intended use The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for separating substances of different densities held in suspension, or emulsion in a liquid.

The maximum density of substances should be 1.2 g/ml.

The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment. Users of the equipment must be trained on good centrifugation practices, as well specific usage of the LOREENA CENTRIFUGE models and Accessories. Any use outside the framework defined above is considered non-compliant. AFI Centrifuge. assumes no responsibility for injury due to improper use.

CAUTION


1.3. Prohibitions: The centrifuge is not designed for the following uses and environments. The following are prohibited:

Flammable, explosive, toxic and radioactive materials

Materials which may react, causing a hazard

Contaminated materials not contained in an aerosol-barrier container

Rotating rotors and accessories that have exceeded their maximum use.

Rotating rotors and accessories that are not maintained according to the instructions in this manual,

or showing signs of wear and/or corrosion.

Rotating rotors and accessories not supplied by AFI Centrifuge.

Explosive environments

Radioactive environments

Only operate a centrifuge after taking all necessary safety measures. Rotors and buckets should be removed from use in case of mechanical fault, trace, or corrosion. These elements have a life cycle duration, engraved on their visible part: To maintain safe conditions, it is imperative to replace them when the recommended duration is reached.

CAUTION


1.4. Models, Specifications and Accessories

1.4.1. Models & Specifications

Description Laboratory Centrifuge

Environmental Conditions For indoor use only Environment Temperature Range: : 60⁰F - 85⁰F (15⁰C - 30⁰C) Environment Humidity: Maximum relative humidity 80% for temperatures up to 31⁰C decreasing linearly to 50% relative humidity at 40⁰C Environment Altitude: 6562 Feet (2000 meters) above sea level

Maximum capacity 4x1000 ml x kg / bucket, 4 x buckets

Models Benchtop Ventilated

Benchtop Refrigerating

Floor Refrigerating

Catalogue Numbers AFI-C300V AFI-C300V-E AFI-C300R AFI-C300R-E AFI-C300RF AFI-C300RF-E

Supply Voltage (Depending on the model)

115 V 10% 60 Hz

230 V 10% 50 / 60 Hz

115 V 10% 60 Hz

230 V 10% 50Hz

115 V 10% 60 Hz

230 V 10% 50Hz

Maximum Power Consumed 1000 Watt 1700 Watt 1700 Watt

Maximum Speed (rpm) 15,300 rpm (with AFI-RA30-2 rotor)

16,000 rpm (with AFI-RA30-2 rotor)

Maximum Relative Centrifugal Force (g)

25,648 xg (with AFI-RA30-2 rotor)

28, 048 xg (with AFI-RA30-2 rotor)

Dissipated Heat (Btuh) (1 Btuh = 0.29307 W)

3412 Btuh 5800 Btuh

Fluid Coolant / Global Warming Potential (GWP)

Not applicable R404A / 3922

Fluid Charge / Ton Equivalent CO2

Not applicable 0.81 kg / 3.18 Teq CO2

Ambient Operating Temperature 20⁰C ± 2⁰C 20⁰C ± 2⁰C

Temperature adjustment range N/A -10 ° C to 40 ° C

Temperature control precision N/A ± 2 ° C

Footprint Dimensions (HxWxD) 37 x 50 x 61 cm 35 x 77 x 63 cm 83 x 57 x 64 cm

Net weight (excluding rotor) 82 kg /181 lbs. 145 kg / 320 lbs. 170 kg / 375 lbs

Packing (H x W x D) / Weight 54 x 74 x 77 cm

97 kg / 214 lbs 54 x 90 x 80 cm

160 kg / 353 lbs 100 x 80 x 80 cm

185 kg / 408 lbs

Maximum sound level 61 dB(A) 60 dB(A) 57 dB(A)

Integrated systems Imbalance detection sensor Motorized lid lock Rotor speed control

Control System Microprocessor

Acceleration slopes 9

Braking slopes 10

Speed control precision ± 10 rpm

Number of programs 99 99 + Pre-cooling + Pre-warming


1.4.2. Accessories

Catalog Number Description Rotor capacity Lot

Rotor & accessories:

NU-RX1000 Swing-Out rotor 4x 1000 ml / One unit

NU-B1000 1000 ml buckets / Set of 4

NU-L1000 Lid 1000 ml / Set of 4

NU-BXDW4 Microplates carriers / Set of 4

Adapters :

AFI-4T1000F 1000 ml Flat Bottle 4 x 1000 ml Set of 4

AFI-4T750F 750 ml Flat Bottle 4x 750 ml Set of 4



AFI-4T500C 500 ml Conical Bottle 4x 500 ml Set of 4



AFI-4T250C 250 ml Conical Bottle (Without Lid bucket) 8 x 250 ml Set of 4


AFI-4T200 200 ml Conical Bottle 8 x 200 ml Set of 4

AFI-4T200 150 ml Conical Bottle 8 x 150 ml Set of 4

AFI-4T100-C 100 ml Conical Bottle 8 x 100 ml Set of 4

AFI-4T50C 50 ml Conical Tube 40 x 50 ml Set of 4

AFI-4T50AOR 50ml AOR Advanced Oak Ridge 40 x 50 ml Set of 4

AFI-4T50S 50 ml Skirted Conical Tube 28 x 50 ml Set of 4

AFI-4T30S 30ml Skirted Conical Tube 40 x 30 ml Set of 4

AFI-4T15C 15ml Conical Tube 96 x 15 ml Set of 4

AFI-4T10 14ml Urine Tube 156 x 14 ml Set of 4

AFI-4T10 10ml Blood Collection Tube 156 x 10 ml Set of 4

AFI-4T5 5-7ml Blood Collection Tube 200 x 5 ml Set of 4

AFI-4T2 1,5 - 2ml MicroTube 200 x 2 ml Set of 4

Set of 4


NU-RX750 Swing-Out rotor 4x 750 ml / One unit

NU-750 750 ml buckets / Set of 4

NU-L750 Lid 750 ml / Set of 4

NU-BXDW3 Microplate Bucket X4 Set of 4

Adapters :


AFI-3T625F 625 ml Flat Bottle (AFI) 4 x 525 ml Set of 4


AFI-3T500C 500 ml Conical Bottle 4 x 500 ml Set of 4




AFI-3T250C 250 ml Conical Bottle (Without Lid bucket) 4 x 250 ml Set of 4





AFI-3T50CL 50 ml Conical Tube 24 x 50 ml Set of 4

AFI-3T50C 50 ml Conical Tube (Without Lid bucket) 28 x 50 ml Set of 4

AFI-3T50AOR 50ml AOR Advanced Oak Ridge 24 x 50 ml Set of 4

AFI-3T50S 50 ml Skirted Conical Tube (Without Lid bucket) 28 x 50 ml Set of 4

AFI-3T50SL 50 ml Skirted Conical Tube 24 x 50 ml Set of 4

AFI-3T30S 30ml Skirted Conical Tube 28 x 30 ml Set of 4


AFI-3T15C 15ml Conical Tube 56 x 15 ml Set of 4

AFI-3T10 14ml Urine Tube 84 x 14 ml Set of 4

AFI-3T10 10ml Blood Collection Tube 84 x 14 ml Set of 4

AFI-3T5 5-7ml Blood Collection Tube 108 x 5 ml Set of 4

AFI-3T2 1,5 - 2ml MicroTube 216 x 2 ml Set of 4


NU-RHDW Microplate Rotor H One unit

NU-BHDW Microplate Bucket H 2 x 4 Microplates

2 x 1 Deep-Weel Set of 2

NU-LH Biosafety secured lid for BHDW set of 2 Set of 2

Maintenance :

71199012 Tube of grease 20gr One unit

Angular Rotor

NU-RA6-100 6 x 100 ml One unit

Adapters

AFI-100R80-OAK 6 x 80ml 80ml AOR Advanced Oak Ridge 6 x 80ml Set of 6

AFI-100R50-C 6 x 50ml 50ml Conical Tube 6 x 50ml Set of 6



AFI-100R15-C 6 x 15ml 15ml Conical Tube 6 x 15ml Set of 6


AFI-100R10 12 x 10ml 10ml Blood Collection Tube 12 x 10ml Set of 6

AFI-100R5 18 x 5/7ml 5-7ml Blood Collection Tube 18 x 5/7ml Set of 6

AFI-100R5 12 x 5ml 5ml MicroTube 12 x 5ml Set of 6

AFI-100R2 18 x 2ml 1,5 - 2ml MicroTube 18 x 2ml Set of 6

Rotor

NU-RA8-50 Angular Rotor 8 x 50 ml One unit

Adapters

AFI-50R50-R 50ml Round Tube 8 x 50 ml Set of 8

AFI-50R50-OAK 50ml AOR Advanced Oak Ridge 8 x 50ml Set of 8


AFI-50R15-C 15ml Conical Tube 8 x 15ml Set of 8


AFI-50R10 10ml Blood Collection Tube 8 x 10ml Set of 8


Rotor

NU-RA30-15 Angular Rotor 30 x 15 ml

Adapters

AFI-15R15-C 15ml Conical Tube 30 x 15 ml

AFI-15R15-R 15ml Round Tube 30 x 15 ml

AFI-15R10-OAK 10ml AOR Advanced Oak Ridge 30 x 10 ml

AFI-15R10 10ml Blood Collection Tube 30 x 10 ml

AFI-15R5 5-7ml Blood Collection Tube 30 x 5 ml

Rotor


Rotor


Adapters

AFI-48R05 0,5ml MicroTube 48 x 2 ml



Rotor


Adapters





1.5. Safety Instructions The operator must observe the following precautions when using the centrifuge:

Ensure stability and perfect levelling of the centrifuge.

Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.

Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and mechanical strength at the centrifugal force applied thereto.

Check the condition of the tubes and remove damaged tubes.

Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge.

Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.

Maintain and control accessories.

Balance the load of the rotor around the axis of rotation.

Respect the maximum density of 1.2 g / ml, in particular in case of a cycle at full speed.

Limit volume in case of excess density.

Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the centrifuge.

During operation, the centrifuge must not be moved or be subject to impact.

Do not attempt to open the lid while the rotor is spinning.

Do not attempt to neutralize the motorized lid lock

Do not lean over the centrifuge during the spin cycle.

Do not stay within a space of 12 inch around the centrifuge longer than necessary.

Do not leave potentially dangerous materials inside the free space.

Use aerosol-barrier accessories when centrifuging bio hazardous material.

Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment. Allow the centrifuge to warm up for two hours before use.

1.6. Information on the refrigerant fluid used The "refrigerant" type apparatus contains fluorinated greenhouse gases under the Kyoto Protocol. They should therefore not be released into the atmosphere. Repairs or disassembly of this appliance should only be carried out by qualified personnel. The data corresponding to the type of refrigerant, the Global Warming Potential (GWP) value, and the load are detailed in § 1.4.1.Models & Specifications.


2. Installation Instructions

2.1. Shipment AFI Centrifuge takes every reasonable precaution to assure that your LOREENA Centrifuge arrives without damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a LOREENA Centrifuge to be sure that if damage has occurred, the proper claims and actions are taken immediately.

2.1.1. Damaged Shipments Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance. If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your filing the claim against the carrier. If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This along with other papers in the customer's possession will support the claim.

2.1.2. Handling and transport The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.). Please ensure that the people in charge of handling are qualified to handle the lifting equipment.

Maintain the following conditions during transport: Temperature: -20 to 50 ° C Relative humidity: <90%, under the dew point

2.2. Site preparation Prepare a stable, clean and levelled support (e.g. laboratory bench). This support must be able to support the weight of the

centrifuge and its use, and be firm enough so as not to generate or maintain vibration. Such vibrations would degrade the results of the centrifugation, and may result in an unbalanced error.

A clearance of 12 inch (300mm) around the centrifuge should be arranged according to IEC 61010-2-020: No potentially hazardous material, nobody or no object shall be deposited within the free space.

Open space around the centrifuge

It is recommended that no less than two people are present to manually lift the centrifuge onto the laboratory bench.

CAUTION

CAUTION

12”

12”

Access area

12”


2.3. Electrical environment The centrifuge requires must be 230V/50Hz or 115V/60Hz, single phase, (current rating varies per centrifuge model, reference Electrical/Environmental Requirements).according to the manufacturer sheet on the back of the unit. The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord. The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes. Do not position the centrifuge to prevent access to the power cord. The power cord plug serves as the main disconnect device and should remain readily accessible. The electrical outlet should be on its own branch circuit, protected with a circuit breaker at the distribution panel near the centrifuge.

2.4. Unpacking Remove the cardboard packaging made of 2 parts (a lid and a bottom). For floor model centrifuge, let a minimum of 1 meter space behind the wooden ramp lied on the floor before getting down the device. The LOREENA Centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the centrifuge, and its spinning accessories, please make sure you are in possession of the following:

2.5. Packing list 1) 2)

or 3)

Or

LOREENA CENTRIFUGE Model

Compliance Certificate QuickStart Guide

1)

2)

3)

Power Cord Manual release key (shape according centrifuge model)

Food grade grease

Centrifuge models : 1) Ventilated or 2) Refrigerating or 3) Floor refrigerating


2.6. Description Fig. 1 Refrigerating centrifuge

1) Lid 2) Window 3) Control panel 4) Electronic warhead 5) Main switch 6) Cooling unit (Refrigerating model only) 7) Electrical outlet

Fig. 2 Floor centrifuge

8) Wheels (x4) 9) Adjustable pads (x2)

2 1

3

5

4

6

7

1

6

4

5

9 8


3. Operating the LOREENA centrifuge - AFI-C300

3.1. First Start / Powering The first start must fulfill the conditions of the IEC 61010-2-020 safety standard

HOW TO GREASE A LOREENA CENTRIFUGE


3.2. Levelling (Floor model)

Mandatory operation : Before using floor model centrifuge, adjust the level setting pads, following the procedure supplied onto the package box (Document Reference AP3.001 Unpacking & installation). Use supplied pads.

3.3. Rotor assembly

Clean and lubricate the motor shaft by depositing a thin film of the food grade grease supplied. Place the rotor above the drive shaft. Slide the rotor on the drive shaft. A click indicates that the rotor is locked. Clean and lubricate the pins by depositing a thin film of the food grease supplied.

3.4. Position the buckets

Swing-out rotors are used with round buckets or micro titration buckets. Place the buckets in the corresponding numbered locations.

3.5. First powering Activate the switch on the bottom right of the control center.

On/Off Switch position

Under Control Center


3.6. Using the interface

3.7. Opening the lid

On the first start, the lid may not open right away due to a long storage in a cold environment. In this case, warm-up the jacks by repeated manual shifting of the lid downwards. To open the lid, press the button on the right side.

3.8. Rotor loading and balancing

Position the 4 numbered buckets in their corresponding positions on the rotor. Each bucket should pivot freely in its slot. Place the inserts corresponding to the tubes to be centrifuged in the four buckets. The buckets must be symmetrically distributed. For better balance, each numbered bucket must be placed in the corresponding position marked on the rotor. Each position on the rotor must be equipped with its bucket. Do not use a rotor without its complete set of four buckets. Maximum loads according to the type of rotor are specified in the annex. The centrifuge tolerates an imbalance of 15 grams. If the imbalance is higher than the tolerated threshold, an imbalance detection system stops the centrifuge automatically. The following message appears: "Imbalance" and the rotor stops in free wheel mode without braking system. Excessive imbalance is likely to damage the turning components and/or the centrifuge.

Loading the buckets

Allowed:

Forbidden :

Loading the rotor

Allowed: Symmetrical loading

Forbidden: Incomplete loading Asymmetrical loading

1. Touch screen for direct access to various functions. 2. Single button for easy operation. This button allows the validation, the start of a cycle, but also the interruption of a cycle, and the output of a menu. The functions accessible through this single button are activated as required by the microcontroller.

2

1


3.9. Quick start-up Before using the centrifuge, make sure it has been properly installed.


4. Operating

4.1. Settings This section is accessible through the Admin section. It allows the creation and/or modification of a program and the customization of the centrifuge.

4.2. Create / edit a program name

Visual Instruction Control

Select the program to create/edit Note: 99 programs are available, from 01 to 99.

ou

The program can be modified when the protection icon is an

open lock:

Edit the title screen by pressing on the title.

Enter the new title Save the new title by pressing "ok". Note: To delete the entire text: To exit the menu without saving changes:

Enter the program number you wish to create Confirm saving Note: Accept without saving changes:

The program has been saved. (The lock is not activated).


4.3. Set program settings


Select the program to edit

or

Example :

Select the desired parameter by pressing the icon:

Acceleration slope (from 1 to 9) 1: slowest acceleration 9: fastest acceleration

Braking slope )from 0 to 9) 0: no braking at all 1: slowest deceleration 9: fastest deceleration

Centrifuge speed (in RPM or RCF)

Cycle time

Setpoint temperature (only on the refrigerating model)

Change the value (e.g. speed). Confirm

Confirm settings Note: To validate without saving the changes in the program, press the "No" button. The middle program then becomes the routine program, noted "..." This data is saved automatically in this routine program.

The program has been saved.

The new set value appears under the pictogram.

Note :

In the case of a temperature setpoint, it is possible to set a

negative value using the key opposite.

The minimum value is -10 ° C.


4.4. Set speed in RPM or RCF


Access the speed setting key

Press on the icon RPM to modify the unit from RPM to RCF.

Enter the RCF (g) value desired, Confirm Note: The rotor name and radius will be displayed below the picture of the rotor.

The default rotor setting is : Swing-out rotor, RX-750 Radius : 184 mm Modify the rotor or radius pressing on the icon Rotor. Note : This icon changes whereas the set rotor is angular or swing-out type.

/

Select the rotor along the list. Note : The "…" icon leads to accurate radius setting menu.

Press onto arrows to access to angular rotors.

Enter the RCF (g) value desired, Confirm

The program will now display the RCF value (g).


4.5. Protect the settings of a program


Select the program to lock

Example :

Press on the open lock for 4 seconds.

The lock is now closed. The program is now protected.

In the list of programs, a lock appears in front of the program. Note: To remove the protection, enter the program through an Admin user profile and press on the closed lock for 3 seconds. It will then re-open. You are now able to modify the program.

4.6. Centrifuge: Use a program Turn on the centrifuge. The switch is in the lower right portion. Any user can access a program (user, admin, service tech)


Select the program you want to use

Or

Load the rotor and buckets as instructed in § 3.2 Close the lid

Start the cycle


Centrifugation starts.

The progression bar appears

A green tick appears when the rotor stops. Acquit pressing onto the screen or start/stop button. Press lid button to open.

or

4.7. Interrupt centrifugation


During a centrifugation, press the Start/Stop button.

The rotor will start slowing down until complete stop A green tick appears when the rotor stops. Acquit pressing onto the screen or start/stop button.

Or

Press lid button to open.

4.8. Open after an electric power failure / Emergency Stop

This operation must be performed by a person trained in centrifugation-related risks. In case of power failure, the lid cannot be opened by the engine unlocking control. You must wait 15 minutes for the rotor to come to a complete stop before opening manually. Floor model : Turn off the power by pressing the switch to the “0” position. Insert the wrench provided fully into the round cavity on the left side. Turn it clockwise to release the lid. Remove the wrench. Unload samples.

2 1 3

CAUTION


1. Benchtop model :

Insert the flat wrench provided into the vertical slot in the blue

part.

2. The wrench must drive an axis inside the centrifuge.

3. Raise up the wrench to start the lid releasing.

4. Remove and upturn the wrench.

5. Insert back the wrench and raise up for complete lid releasing.

Following a manual release, the wrench must be removed from its housing to prevent accidents related to the rotation of the wrench during the activation of the locking mechanism. In addition, the security indicator label must be replaced during a safety check carried out by an authorized technician.

4.9. Precooling On the Refrigerating model, a "Pre-cooling" cycle prior to centrifugation allows to cool the centrifuge down to 4 degrees Celsius so it is ready for temperature sensitive samples.


cooling

Select the pre-cooling program: Rotor: Standard Precool

This program is set to cool the centrifuge and accessories down to 4°C during a 22 minute cycle at 2500 RPM. Acceleration and Deceleration slope are both set on 9 and cannot be modified.

Air: Smart Precool

This program is set to cool the centrifuge until the probe temperature reaches 4⁰C and will stop when the temperature is reached.

*Select Rotor or Air in user preferences Start the cycle by pressing The temperature inside the bowl will show on the display

4.10. Prewarming On the Refrigerating model, a "Pre-warming" cycle prior to centrifugation allows to warm the centrifuge so it is ready for a protocol at ambient temperature or warmer (especially after a cycle at a colder temperature).


2

2 1 3


Access to the list of programs by pressing this icon :

Select the pre-warming program:

Start the cycle by pressing The temperature inside the bowl will show on the display

This program is set to warm the centrifuge up to 20°C during a 20min cycle at 3,500 RPM. Acceleration and Deceleration slope are both set on 9 and cannot be modified. Start the cycle by pressing The temperature inside the bowl will show on the display

4.11. Routine Menu The routine program allows you to modify the cycle parameters without seeing the automatic request for backup at each change. It is therefore necessary to press the "Save" key to save the parameters.


Select the program "0" or "...". It is possible to modify the parameters. If no backup is performed, the settings will not be saved when the power is turned off.

4.12. Other menus



Access to other menus by pressing :

Select the menu

User Preferences

Service menu

Information

Go back to the centrifugation screen.


4.12.1. User Preferences Visual Instruction Control

Access the « User Preferences » menu via the following icons:

;

Type of speed displayed: RPM or RCF (G force)

Frequency of Lubrication

Logbook

Error logbook

Type of temperature displayed : Celsius or

Fahrenheit

Timer settings

Counting mode

Keyboard type

Background Color

End of Cycle Alarm

Error Message Alarm

Automatic lid opening

Sleep mode

Post Cool

Pre-Cool Function

Temperature Display Mode


4.12.2. Type of Speed displayed (RPM or RCF)


Select the unit you want displayed by default:

RPM: Rotation Per Minute

RCF: Relative Centrifugal Force, or G Force

Confirm Do not turn the centrifuge off at this point You now have to restart the unit.


4.12.3. Frequency of lubrication


Select how many cycles you want between each lubrication reminder.

No reminder (Not recommended)

100 cycles (Default value)

200 cycles

300 cycles

Confirm

4.12.4. Access to logbook


The counters keep track of the life of the centrifuge.

CYCLE COUNT: Quantity of cycles on the centrifuge.

SWING-OUT CYCLE COUNT: Quantity of cycles ran with a swing-out rotor

SWING-OUT CYCLE COUNT >4,000RPM: Quantity of cycles ran with a swing-out rotor faster than 4,000RPM

RUNNING HOURS: Amount of hours the centrifuge has been used.

COLD MODULE RUNNING HOURS : Amount of hours the refrigeration has been running

COLD MODULE STARTS : Number of time the refrigeration has started (compressor)

LID OPENINGS: Number of lid openings

Return

4.12.5. Access to error logbook


The error logbook makes it easier to service and do maintenance on the unit.

The messages are recorded per error number, making it easier to save all the errors and how often they happen. For more details on these error codes, refer to section 6.2.22. Return


4.12.6. Type of Temperature displayed


Select you unit you want displayed by default:

°C: Degree Celsius

°F: Degree Fahrenheit

Confirm

4.12.7. Timer mode


Select the type of timer:

ON: The timer only starts when the centrifuge has reached the set speed in the program

OFF: The timer starts right when you

start the cycle (Press .)

Confirm


4.12.8. Counting mode This function is used to display counting up or down during

centrifugation.

Select the counting mode of the stopwatch. All programs will work according to the chosen mode.

• UP: The stopwatch counts from 0.

• DOWN: The stopwatch counts down from the selected cycle time. • This is the default setting.

4.12.9. Keyboard type This function is used to set keyboard type.

Select keyboard type :

QWERTY.

AZERTY: This is the default setting.

QWERTY / AZERTY keyboards

Keyword capital letters

Access numbers & special characters.


4.12.10. Change background color You can modify the background color of the display

Choose the color you want. Confirm

4.12.11. End of Cycle Alarm This function activates a beep when the cycle is complete.

ON: Upon stop the signal is triggered

Pressing the key stops the signal.

OFF: No signal at the end of the run. This is the default.

4.12.12. Error Message Alarm This function activates a beep when an error message appears

ON : Upon error the signal is triggered

Pressing the key stops the signal.

OFF: No signal upon error. This is the default.

4.12.13. Automatic lid opening Visual Instruction Color

The "AutoLid" function allows the automatic opening of the lid at the end of a cycle when the rotor stops. Pressing the lid opening button is no longer necessary.

ON : The lid automatically opens at the end of a cycle.

OFF : At the end of the cycle, the lid opens only after pressing the lid opening button.

This is the default setting.

Validate


4.12.14. Sleep mode This function allows the screen to be paused from the Stand-By screen.

ON: The lid opens automatically at the end of the cycle.

• OFF: at the end of the cycle, the lid opens only after pressing the lid opening button. This is the default setting.

Validate.

4.12.15. Post Cool This function maintains the temperature in the bowl between

runs.

ON: After closing the lid the refrigeration, the unit will maintain the temperature for 4 hours.

Note: Upon opening the lid the 4 hour timer will restart.

OFF: Bowl temperature is not maintained between runs.

Validate

When Post Cool is activated this symbol appears:

- On the main screen next to the thermometer.

- On the Precool screen right of the temperature.


4.12.16. Pre Cool Function

Allows Pre Cool to 4⁰C in air or rotor mode.

AIR: Cooling to the temperature in the bowl. This is the default.

ROTOR: Cooling for 22 minutes. At this point the rotor and accessories are cooled.

4.12.17. Temperature Display Mode

Allow for display of air or rotor temperature.

AIR: The temperature displayed is the temperature of the air in the bowl. This is the default

ROTOR: The temperature displayed is that of a sample in a tube. The sample must have been previously stored at the same

temperature.

During Centrifugation: If the Rotor mode is selected the tube appears. If the Air mode is selected no logo appears. Pressing the temperature button can temporarily change the display mode.


4.13. Service Menu

4.14. Information


Access the menu through this icon :

This menu provides general information on the Software and Hardware of the centrifuge : SERIAL NUMBER: Unique identification number of the centrifuge Centrifuge fw: Software version of the microcontroller Display fw: Software version of the display µController Board Id: ID of the microcontroller electronic board Power Board Id: ID of the electronic power board Accelerometer Board Id: ID of the electronic board of the accelerometer Return

4.15. Energy saving The "Postcool" function makes it possible to maintain a temperature in the chamber of the tank, after the rotor has stopped. Stopping this function prevents over-consumption of electricity. This function is disabled ("OFF") by default.


This menu is only accessible to certified service companies. Exit

An access code is required. Only certified technicians are allowed to go further.


5. Care and Maintenance

All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedures required for this equipment

The device and its accessories may be contaminated. Apply decontamination measures required before any maintenance. Turn off the centrifuge power switch. Ensure you lock the switch when working on the unit, to avoid any accident from a third party. Special care must be provided to maintain the original terms of safety and performance, to extend the life of accessories. A visual inspection can identify any signs of wear. An annual inspection may be requested according to relevant regulations.

5.1. Care and Cleaning

5.1.1. Authorized maintenance products Users should not use cleaning or decontamination methods different from those recommended by the manufacturer, as they may cause damage to the unit.

The following products and materials are allowed for maintenance:

- Distilled water, - 70% IPA, peroxides or quaternary ammonium surface disinfectants. - Lint-free cloth - Soft non-metallic brush - Lanolin or silicone spray

Forbidden products and materials:

- Chlorine-containing products (Bleach, chlorides, ...) - Saltwater - Wire brush

5.1.2. Centrifuge Perform regular cleaning of the following parts to prevent any risk of corrosion in case of persistent impurity, and to ensure the hygiene of the working equipment.

External parts lid & keyboard:

- Use fluids and tissues impregnated with hydro-alcoholic solution or quaternary ammonium - Check that the air vents are not obstructed.

Centrifuge chamber:

- Remove the rotor before cleaning the tank. - Dry thoroughly after cleaning. - Do not use fluffy fabric. - Replace the rotor after cleaning the tank.

CAUTION

CAUTION


5.1.3. Rotor & Accessories Use clear or distilled water to clean the rotor and buckets. A soft non-metallic brush or lint-free cloth can be used. Dry the rotor and accessories ensuring the least accessible areas are wiped.

Image Operation

Fig. 1 Rotor

To ensure a proper swinging movement of the buckets, clean all 8 trunions of the rotor.

Fig. 2 Trunions

Remove the leftover grease and dust. Apply a thin layer of food quality grease (provided).

Also clean the buckets, more specifically the area in contact with the trunions.

Make sure to set the “greasing reminder” in the User Preferences menu. To prolong the life of accessories, you can then place a thin film of silicone spray or lanolin. Rotors, buckets, inserts can be autoclaved at 122.5 ° C / 252 ° F / 215 kPa for 15 minutes

Note: When autoclaving, buckets, inserts, and rotors must be separated from each other. Autoclaving causes accelerated aging of plastics, and may change the color of the plastic. Perform regular cleaning to prevent any risk of corrosion in case of persistent impurity, and to ensure the hygiene conditions of working equipment.

5.1.4. Disinfection / Decontamination Bio hazardous, radioactive and/or toxic materials: Before any intervention, to ensure the safety of the operator, please refer to the laboratory’s decontamination protocol. The centrifuge and its accessories may be contaminated and require remediation in this case.


5.2. Dismantling the rotor

Image Operation

Open the lid. 2.Grab the red wheel on top of the rotor and turn it counterclockwise while holding the rotor firmly. Once you hear a “click” sound, the rotor is unlocked

1. 3.Grab and lift the rotor with both hands once it’s unlocked.

Set the rotor on a flat surface or on one of AFI Centrifuge’s rotor stand.

5.3. Limiting the duration of operation The use of accessories and the rotors is limited in time to ensure the integrity of their mechanical strength. These limits are marked on the accessories.


When there is only 2,000 cycles left before the end of the theoretical life time of the rotor, this screen will be displayed. It shows the amount of cycles left before the rotor should stop being used (rotor and buckets). This value will decrease by 1 each time the rotor is being used. You can still use the centrifuge, but only as long as the value is higher than 0. Anticipate the purchase of a new set of rotor + buckets in the near future.

Once the rotor has reached the end of its theoretical life time, the screen will inform you that the rotor cannot be used. The rotor and buckets are obsolete and have to be replaced promptly. A certified technician has to configure the centrifuge for the new rotor.

2

3


5.4. Checking and testing accessories

Check that no trace of corrosion appears. Check also the absence of any mechanical damage (traces of impact, corrosion, cracking), or wear on the rotor and bucket.

In order to ensure optimal performances, once the accessories have reached half of their theoretical life time, we recommend

inspecting them. This life time is different from one accessory to the other, and the value is engraved on the accessory (i.e.:

Max cycles: 55,000 means that the accessory has a theoretical life time of 55,000 cycles)

For safety reasons, the use of accessories that have reached the end of their life cycle is prohibited. These components must

be replaced.

5.5. Storage of accessories Do not store the accessories on a wire rack. Oxidation build-up may appear and show premature wear of accessories

5.6. Annual maintenance A periodic check of the centrifuge and accessories is required. Checks must also be carried out in accordance with the

regulations in force: Functional (lubrication of the drive shaft, the lock, readability of labels), security (continuity of the

protective ground connection, checking & maintenance of rotors and accessories) and centrifuge performance checks are

recommended at least once a year.

Replacement of consumables (grease, provided)

Check that the grounding of the device is always properly secured by its power cable and the wall outlet it is connected to.

The operator is not authorized to access the internal part of the equipment. An AFI Centrifuge authorized technician will

ensure maintenance.

CAUTION


5.7. Unbalancing sensitivity detection The centrifuge has a reaction to the load balancing faults which may be different depending on the media on which the centrifuge is placed. A calibration of the imbalance sensitivity is therefore necessary during installation. Required equipment: Use the reference balancing kit AFI-71122002.


Access the unbalance sensitivity calibration menu.

Equip a rotor swing-out and its 4 nacelles in the centrifuge. Remove the inserts of the nacelles.

Low threshold

1. Place the round 15 gram weight in one of the nacelles and close the lid,

2. Press the button “15gr” 3. The rotor accelerates until it stabilizes. 4. The centrifuge shakes, then the rotor slows down. 5. The low threshold is then determined. It appears under the

button “15 gr”. (e.g. 480) 6. Open the lid and remove the 15 gram weight.

High threshold

7. Place the 25 gram weight in the same nacelle. 8. Proceed in the same way as for the 15 gram weight. 9. The low threshold is then determined. (e.g. “520”) 10. Open the lid and remove the 25 gram weight.

Average

11. Press on the Next button to average the setting. (e.g. [480+520]/2 = 500 ) The average value is displayed. By default, the factory setting value is 500.

Check The following steps are COMPULSORY. High threshold:

12. Place the 15 gr + 10 gr weights in a nacelle. 13. Start the cycle. 2000 rpm / 1 minute / 9 Acceleration /

Braking 9 14. The centrifuge must stop during the acceleration, and

display the message ERROR1: The 25 gr imbalance is not tolerated. Repeat the High threshold check 3 times: ERROR1 must appear each time.

Do not adjust this threshold with a weight greater than 25 gr. This would cause a breach of warranty. The

appliance has been dimensioned for a maximum threshold of 25gr.


Low threshold:

15. Leave only a weight of 15 gr in a nacelle. 16. Start the cycle. 2000 rpm / 1 minute /

9 Acceleration / Braking 9 17. The centrifuge should achieve the desired speed and then

slow down and stop without the following error message appearing: The 15 gr imbalance must be tolerated. Repeat the Low threshold check 3 times: Each cycle should take place normally.

5.8. Error Messages, Troubleshooting, Option-Diagnostics

Visual Instruction

In case of a problem, the display shows an error message and an error number.

Error / Message Cause Solution

01 LOCK FAIL The lid is open when the centrifuge starts. Close the lid. Wait for complete closing of the lid before starting a new cycle.

The lid sensor is malfunctioning. Contact a Certified Technician. 02 IMBALANCE The rotor buckets are not loaded

symmetrically. Wait until complete stop. Open the lid. Load the buckets as shown in manual. Start a new cycle.

The centrifuge is installed on an uneven bench.

Install the centrifuge on a flat bench/table, as described in manual.

The imbalance detector is not well calibrated. Contact a Certified Technician. 03 BOWL

OVERTEMPERATURE The temperature in the bowl is higher than 43C; the room temperature is too high.

Wait until complete stop. Turn the AC or Ventilation system on to obtain an ideal ambient temperature as described in manual.

The temperature set in the program is not adapted.

The settings for speed / temperature are not compatible with a normal utilization.

The temperature of the samples is too high. Make sure the samples can be cooled to down to a temperature between 4C and 37C during 1 hour

The refrigeration doesn’t work. Contact a Certified Technician. The temperature probe is malfunctioning. Contact a Certified Technician. The difference between the air temperature and the over temperature alarm set point is greater than the set value.

Check the value of the temperature alarm threshold. Change program settings: speed and/or temperature.

04 MOTOR OVERTEMPERATURE

The motor temperature is too high. A « no-brakes » stop is occurring. Wait 30 minutes before opening the lid. Turn the AC or Ventilation system on to obtain an ideal ambient temperature as described in manual.

The centrifuge is being used too intensively (ventilated model).

Let the centrifuge cool down between cycles.

The motor has been damaged Contact a Certified Technician. The temperature sensor on the motor has a bad connection.

Contact a Certified Technician.


05 ERROR MICROCONTACTOR ON LOCKING MECHANISM

One of the micro contacts is damaged or non-working. There is a bad connection to the micro contacts.

Contact a Certified Technician.

06 MICROCONTACT NOT SEEN - OPEN

The micro contact doesn’t work. The auxiliary micro contact is not working. The connection of the auxiliary micro contact is not good.

The micro contact was seen as closed when it should have been seen as open when the centrifuge started. Contact a Certified Technician.

07 MICROCONTACT NOT SEEN – CLOSED

The power contactor doesn’t work. The open position switch was released after the lid closed. The micro contact doesn’t receive a command. The coil of the micro contactor is not working properly.

The micro contact was seen as open when it should have been seen as closed. Contact a Certified Technician.

08 ERROR LID SAFETY The board with tachometer / accelerometer is working improperly. The connection to these parts is not good. The sensor for the closed lid position was released after the centrifugation started.

The lid slightly opened during the security auto test when the cycle started.

09 NO SPEED SIGNAL AT START

The speed sensor is working improperly. The board with tachometer / accelerometer is working improperly. The connection to these parts is not good.

The speed cannot be read within the first 5s of the cycle.

10 SPEED SIGNAL LOST The speed sensor is working improperly. The board with tachometer / accelerometer is working improperly. The connection to these parts is not good.

The speed signal was lost during rotation.

11 WRONG SPEED SIGNAL

The speed sensor is not working properly. The board with tachometer / accelerometer is working improperly. The connection to these parts is not good. Magnets from the tachometer might have been lost.

The speed signal is not correct.

12 OVERSPEED Major error, the centrifuge will stop. The speed measured is higher than the maximum speed allowed for this rotor. Contact a Certified Technician.

13 Bus I²C Accelerometer

The board with tachometer / accelerometer is working improperly. The connection to these parts is not good. The microcontroller board is working improperly.

Problem of communication with the accelerometer.

14 Bus I²C Temperature probe

The microcontroller board is working improperly.

Problem of communication with the temperature measurement.

15 LID OPEN DURING ROTATION

Manual lid opening during centrifugation. Wrong calibration of locking mechanism.

The lid was manually opened or the hook was released during the centrifugation. Contact a Certified Technician.

NO DISPLAY No power. Verify the power plug is connected properly. Check the power in the laboratory. Turn the switch on.

16 TEMPERATURE CONTROL ISSUE

Temperature probe out of service. Verify that the temperature probe is properly installed under the lid and replace it if necessary. Remove the probe and measure the resistance. The value must be 500+ - 100 Ohm (Probe PT500)

17 ERROR OVERSPEED SAFETY

The startup test of the overspeed safety has failed.

Microcontrol board is defaulting: Replace the control center.

In case of malfunction, contact after-sales service if the previous table does not eliminate the error. You will be asked for the type of centrifuge and the serial number for more effective troubleshooting.


Never try to disassemble or fix the unit on your own: the risks of getting injured or electrocuted are high. The manufacturer warranty would then be void. Only a certified technician is authorized to perform such operations.


5.9. Wearing parts & consummable Reference Designation Comment

Ventilated model

71122500 Gas spring kit (ventilated model) ESL : 3 years

71122501 Upper Bowl seal kit - (ventilated model) ESL : 5 years

71122502 Motor seal kit - (ventilated model) ESL : 3 years

71122503 Shock Absorber kit (ventilated model) ESL : 5 years

Refrigated

Model

Benchtop

71123000 Gas spring kit - R (jeu de 2) ESL : 3 years

71123001 Upper Bowl seal kit – R - (benchtop & floor) ESL : 5 years

71123002 Motor seal kit R - (benchtop & floor) ESL : 3 years

71123003 Shock Absorber kit - R ESL : 5 years

Floor Model

71123100 Gas spring kit ESL : 3 years

71123101 Shock Absorber kit - RF ESL : 5 years

71199012 Grease tube for accessories – 20 gr Consumable

71199003 Disinfectant Consumable

ESL: Estimated Service Life, in years, before change, for preventive maintenance, in the case of use, maintenance and maintenance in accordance with the operating manual. Otherwise, or for intensive use, these lifetimes can be reduced.

5.10. Back to manufacturer In case of a need to return to AFI Centrifuge manufacturer of the device and / or its accessories, all elements must be decontaminated before transport, in order to protect the various parties involved and the environment. In the event of non-compliance, we retain the right to refuse receipt of the equipment. Any return must be documented with a decontamination certificate. Cf. § 8.4 Return coupon.


6. Electrical/Environmental Requirements

6.1. Electrical (Supply Voltage Fluctuations Not to Exceed +/- 10%)

AFI-C300V 115V 60Hz 9 Amp 1 Phase UL/UL-C Listed

AFI-C300R 115V 60Hz 17 Amp 1 Phase UL/UL-C Listed

AFI-C300V-E 230V 50Hz 4 Amp 1 Phase CE Certified

AFI-C300R-E 230V 50Hz 8 Amp 1 Phase CE Certified

AFI-C300RF 115V 60Hz 17 Amp 1 Phase CE Certified

AFI-C300RF-E 230V 50Hz 8 Amp 1 Phase CE Certified

6.2. Operational Performance (for indoor use only) Environment Temperature Range: 60⁰F - 85⁰F (15⁰C - 30⁰C) Environment Humidity: Maximum relative humidity 80% for temperatures up to 31⁰C decreasing linearly to 50% relative humidity at 40⁰C Environment Altitude: 6562 Feet (2000 meters) above sea level

6.3. Light Exposure Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.

6.4. Installation Category: 2.0 Installation category (overvoltage category) defines the level of transient overvoltage, which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.

6.5. Pollution Degree: 2.0 Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

6.6. Chemical Exposure Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting. CHLORINATED AND HALOGEN MATERIALS ARE NOT RECOMMENDED FOR USE ON STAINLESS STEEL SURFACES.

6.7. EMC Performance (classified for light industrial) Emissions: EN61326 Immunity: EN61326

Class A equipment is intended for use in an industrial environment. In the documentation for the user, a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due to conducted as

well as radiated disturbances.

WARNING


7. Disposal and Recycle

Before disposing the equipment, it must be decontaminated and cleaned to protect people, the environment and equipment. Legal regulations must be observed when disposing of the unit.

Electrical appliances are governed by national regulations based on the 2002/96/EC European Directive on electrical and electronic equipment waste WEEE. According to this directive, no equipment supplied after August 13, 2005 in the Business-to-Business sector - which includes this centrifuge - must be disposed of with household waste. For easy identification, the appliances are marked with the symbol displaying a crossed out dustbin.

Centrifuges that are no longer in use and are ready for disposal contain reusable materials. ALL components may be disposed and/or recycled after they are known to be properly disinfected.

Prior to any disassembly for disposal, the centrifuge must be decontaminated.

Component Material Main Cabinet Painted Steel Bowl Stainless Steel Inner Lid Plastic Motor Various Steel/Copper Printed Wiring Assembly Lead Free Electronic Wire PVC Coated Copper Connectors Nylon Hardware Stainless Steel and Steel

Note: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.

BIOHAZARD

RECYCLE

LEAD FREE

CAUTION !


8. Theoretical Basics of Centrifugation

8.1. Basics Relative Centrifugal Force In customary practices and usages of centrifugation, the quantization unit of angular velocity (denoted N), is the revolution per minute (abbreviated rpm, RPM, r / min, or r • min-1). This is a measure of frequency of rotation. It corresponds to the number of full rotations completed in one minute around a fixed axis. The derived frequency unit for SI units is Hertz, with the symbol Hz. Its expression in terms of SI is s-1.

1.60

21 sradrpm

Laboratory centrifuges are used to separate solids of different densities in suspension, applying the relative centrifugal force (RCF for short) on samples. The effective force increases with the square of the rotational speed and distance from the axis of rotation. This force, known as "g-force" is quantified as the number of "g" applied to the sample. No SI units are provided. The following formula is used to convert the angular speed to "g-force"

g

rNRCF

5

22

10.9

RCF Relative Centrifugal Force, "g" N: angular velocity, revolutions per minute r: radius of the circular path of the sample, mm g: acceleration of standard gravity, 9806 65 meters per second per second (ms-²) The relative centrifugal force depends on the speed and the rotation radius. An approximation, denoted F, is used in practice to calculate the value of the relative centrifugal force:

2

1000118.1

NrF

Speed, based on the relative centrifugal force is calculated by the following formula:

r

FN

118.11000

Integral spin It is the accumulated relative centrifugal force acting on the centrifuged object at the corresponding time. It is outlined by the colored surface of the chart below.

RCF

Duration


8.2. Nomogram

The nomogram graphically connects by a line segment a speed to the relative centrifugal force (RCF) as a function of the radius of rotation selected. In the example above, the speed of 2500 rpm corresponds to a relative centrifugal force of 550 g for a radius of rotation of 79 mm

Radius (mm) RCF (Number of grams) RPM (r/min)


8.3. Logbook Example of table to monitor the use of the centrifuge and accessories.

Date Operator RPM RCF Duration T° Rotor Incidents


8.4. Return coupon Return Authorization No. BRaammdd

A return number will be provided after contacting us. In order to process your file, we ask you to return this document duly completed by you. This document must be affixed outside the package. Within 72 hours of receipt of material, we will send you an acknowledgment of receipt.After diagnosis by our technicians, we will send you an offer of price.

Your contact details

Company / Laboratory:

Interlocutor:

Site Address

Post code Town

Country

Phone

Email Address

Billing address

(If different from the site address)

Town

AFI Centrifuge Contact information

Society AFI CENTRIFUGE

Address : ZI Nord Bazouges

Post code 53200 Town Château-Gontier

Description of your equipment

Designation Ref. Catalog

Serial No

List of accessories attached to your equipment

Reason for return

Decontamination

Agent used /

Duration of application

Operator /

Remarks It is your responsibility to properly protect the equipment and to use suitable packaging. Any material that is received damaged or damaged as a result of improper packaging will not be guaranteed. Warranty claims will only be validated by our technical service after inspection of the device. The guarantee will not be applicable in case of incorrect handling of the user or deteriorated elements due to misuse (For more details, see our general conditions of sale). If your equipment does not meet the hygiene requirements for its handling, we will be obliged to carry out its decontamination. This service will be charged according to our current rates. In case of refusal of your part of the Service quote (repair or replacement), the expenses of file will be invoiced to you according to our rates in force.


9. Annex 1: Accessory Specifications

9.1. Swing-Out Rotor NU-RX1000 Rotor

Part number: NU-RX1000

Description: 4 liters swing-out rotor

Maximum capacity: 4x1000 ml

Fastening: ClickSpin system

Maximum rotor speed 3500 rpm

Maximum centrifugal force of the rotor

2750 xg (with NU-B1000 buckets)

Lifetime (Indicative duration)

50,000 cycles 8 years

Centrifuge performance Maximum speed Maximum centrifugal force

AFI-C300 Ventilated 3500 rpm 2750 xg

AFI-C300 Refrigerating 3500 rpm 2750 xg

Buckets

Part number: NU-B1000

Description : 1 000 ml bucket (Set of 4)

Maximum speed 3500 rpm

Maximum RCF 2750 xg

Maximum capacity 1750 grams



Maximum Radius(***) : 201 mm

Lid

Part number: NU-L1000

Description: Sealed lid for 1000 ml bucket

(Set of 4)

Inserts (Sets of 4)

Part number Radius(*) Capacity per insert Total capacity

(Rotor)

410NU-2T5 196182 mm 100023x Tubes 5/7 ml Round bottom

92


92


92

4500CNU-2T5 196182 mm 50023x Tubes 5/7 ml Round bottom

92

290F 183182 mm 229023x Tubes 5/7 ml Round bottom

8


4250CNU-2T5 198182 mm 2250Conical (Without

Lid Bucket)23x Tubes 5/7 ml

Round bottom

8

4200FNU-2T5 193182 mm 220023x Tubes 5/7 ml Round bottom

8

4200NU-2T5 198182 mm 2200

2x150

Conical23x Tubes 5/7 ml

Round bottom

892

4100CNU-2T5 198182 mm 2 100Conical23x Tubes 5/7 ml

Round bottom

092

450CNU-2T5 198182 mm 050Conical23x Tubes 5/7 ml

Round bottom

092

450AORNU-2T5 196182 mm 050 23x Tubes 5/7 ml Round bottom

092

430SNU-2T5 185182 mm 1030 23x Tubes 5/7 ml Round bottom

092

415CNU- 196182 mm 2415Conical 23x Tubes 5/7 ml

Round bottom

9692

410N 188182 mm 39x0 Blood Collection

Tube39

156

45N 188182 mm 505-7Blood Collection

Tube

200

42N 182182 mm 50 200

(*) The radius is the distance between the rotor shaft and the bottom of the insert holes when the bucket is spinning in a horizontal position (during centrifugation)

Microplate carrier

Part number : NU-BXDW4

Description: Microplate carrier for NU-RX1000 rotor (Set of 4)


Maximum RCF 2655 xg

Microplate capacity 6 x 96 wells

Deepwell block capacity 1 x 96 wells

Maximum capacity : 800 grams




Maximum Radius : 194 mm

Included :

Part number : NU-TXDW4

Description: Stainless Steel Extractor & Neoprene pad (Set of 4)

(***)The radius is the distance between the rotor shaft and the bottom of the microplate carrier when the carrier is spinning in a horizontal position (during centrifugation)

Microplate carrier lid

Picture not available Part number : NU-LXDW4

Description: Lid for microplate carrier (Set of 2)

Extractor & pad



Configurations:

Loadings

Each bucket must be loaded evenly.

Allowed:

Forbidden:

Loading the rotor

The rotor must be loaded evenly.

Allowed:

Forbidden:

Note : A message appears when choosRX1000 rotor.

2x NU-B1000 loaded buckets

(inserts & loaded tubes)

2 NU-B1000 buckets

With empty inserts.

2xNU-B1000

chargées

(portoirs & tubes

pleins)

2 nacelles NU-

BXDW4 chargés

2 nacelles NU-B1000

loaded buckets


2xNU-B1000 empty

buckets (ex: without

insert)

2xNU-B1000 loaded buckets


2xNU-BXDW4 empty

buckets (ex: without insert)

Symmetrical loading Asymmetrical loading Incomplete loading


9.2. Swing-Out Rotor NU-RX750 Rotor

Part number: NU-RX750

Description: 3 liters swing-out rotor

Maximum capacity: 4x750 ml


Maximum rotor speed 4600 rpm (with NU-B750 buckets)


4730 xg (with NU- B750 buckets)



Centrifuge performance Maximum speed

Maximum centrifugal force



Accessories

Buckets

Part number : NU-B750

Description: Round bucket 750 ml (Set of 4)

Volume 750 ml



Maximum centrifugal force 4730 xg



Maximum Radius 200 mm

Lid

Part number: NU-L750

Description : Airtight lid 750 ml (Set of4)

Inserts (Sets of 4)

Part number Radius (*)

Capacity per insert

Total capacity (Rotor)

AFI-3T625F 198 mm

1x 625 ml Bottle Flat bottom

4

AFI-3T500C

198 mm 1x 500 ml Bottle Conical bottom

4

AFI-3T500F

… mm 1x 625 ml Bottle

Flat bottom 4

AFI-3T400F 198 mm 1x 400 ml Bottle

Flat bottom 4


Flat bottom 4

AFI-3T250C 200 mm 1x 250 ml Bottle Conical bottom

4


AFI-3T250F … mm 1x 250 ml Bottle

Flat bottom 4


4


Flat bottom 4


4

AFI-3T50CL 200 mm 6x 50 ml Tubes Conical bottom

24

AFI-3T50C 197 mm

7x 50 ml Tubes Conical bottom

(Without Lid bucket)

28

AFI-3T50AOR 198 mm 6x AOR Tubes Advanced Oak

Ridge 24

AFI-3T50S 197 mm

7x 50 ml Tube Skirted bottom

(Without Lid bucket)

28

AFI-3T50SL 198 mm 1x 50 ml Bottle Skirted bottom

24

AFI-3T30S 197 mm 6x 30 ml Tubes Skirted bottom

24

AFI-3T15C 198 mm 14x 15 ml Tubes Conical bottom

56

AFI-3T10 198 mm

21x Tubes 10 ml Blood Collection

Tubes 21x Tubes 14 ml

Urine Tubes

84

AFI-3T5 197 mm 27x 5-7 ml

Blood Collection Tubes

108

AFI-3T2 198 mm 2x57x MicroTubes

1.5 / 2 ml 216


Support microplaque

Part number : NU-BXDW3

Description: Microplate carrier for NU-RX750 rotor (Set of 4)


Maximum RCF 4564 xg

Microplate capacity 6 x 96 wells

Deepwell block capacity 1 x 96 wells




Maximum Radius : 193mm

Included :




9.3. Swing-Out Rotor NU-RHDW Rotor

Part number: NU-RHDW

Description: Microplate swing-out rotor

Maximum capacity: 2x 4 96-well microplates 2x 1 96-well "Deep Well" plate


Maximum rotor speed 5900 rpm (With NU-BHDW buckets)


6072 xg (With NU-BHDW0 buckets)

Lifetime 30,000 cycles at maximum speed 6 years




Accessories for NU-RHDW

Buckets

Part number: NU-BHDW

Description: Micro titration buckets (Set of 2)

Capacity standard Microplates 4 plates per bucket (total of 8)

Capacity Deep well blocks 1 block per bucket (total of 2)


Lifetime 30,000 cycles at maximum speed 6 years

Maximum radius : 156 mm

Included :

Neoprene pad (Set of 2)

Stainless Steel Extractor (set of 2)

Lid

Part number: NU-LH

Description : Airtight lid for Microplate (Set of 2)

Configurations:

Loading the buckets

Allowed:

Not allowed:

Loading the rotor:


Forbidden : Incomplete loading

Asymmetrical loading


9.4. Angular Rotor AFI-RHD3 Rotor and accessories only available on the "VT" series, according to the following references:

AFI-C300RE-VT

AFI-C300R-VT

AFI-C300RFE-VT

AFI-C300RF-VT

Rotor

Part number: AFI-RHD3

Description: Swinging Bucket Rotor

Maximum capacity: 2x 3 kg


Maximum rotor speed 3700 rpm (With AFI-BHD3 carriers)


2510 xg (With AFI-BHD3 carriers)

Lifetime 30 000 cycles

(6 years)

Centrifuge performance Maximum speed Maximum

centrifuge Force

AFI-C300 Ventilated Not affected Not affected

AFI-C300 Refrigerated 3700 rpm 2510 xg

Accessories

Microplate carriers (Set of 2)

Part number: AFI-BHD3

Description: Microplate carrier

Capacity standard Microplates

56x 5 ml tubes

Maximum capacity : 1900 g

Lifetime 30 000 cycles

(6 years)

Radius 161 mm

Adapter (Sets of 2 adapters / Pack of 4 matrix)

Part number: AFI-3TD5

Description: Veterinary Adapters pack

Adapter Made of 2x joined matrices, fixed by

slide "dovetail" & magnet

Adapter capacity 112 tubes

Adapter Dimensions (LxlxH) 174.5 x 266.25 x 57 mm

(Joined matrices dimension)

Radius (*) 164 mm

Rotor capacity 224 tubes

Single Matrix detail 56x 5 ml tubes

BD Vacutainer Type

8x7 tubes, Ø 13.5 mm

174.5 x 134.5 x 57 mm,

matrix of 18.9 mm gap

(*)Radius corresponds to the distance between the center of rotation and the bottom of the tube adapter housing, the

nacelle being in horizontal position (centrifugation position).

Automatic Tube Opening (Set of 2)

Part number: AFI-3TD5-E

Description: Automatic Tube Opening System

Adapter capacity 56x 5 ml tubes

Rotor capacity 112x 5 ml tubes

Cap compatibility Incoming & Outgoing


Configurations:

Loading the buckets

Allowed:

Not allowed:

Loading the rotor:


Forbidden : Incomplete loading

Asymmetrical loading


Angular rotor NU-RA24-2


9.5. Angle Rotor NU-RA8-50

Rotor

Part number: NU- RA8-50

Description: 8x 50 ml Angle rotor

Maximum capacity: 8x 50 ml Conical tubes

Maximum load 8 x 67 gr

Micro tube tilt: 25°




16660 xg

Centrifuge performance Maximum speed

Maximum centrifugal force



Maximum radius 102 mm


9.6. Angle Rotor NU-RA30-15 Rotor

Part number: NU-RA30-15

Description: 30 x 15 ml Angle rotor

Maximum capacity: 30x 15 ml micro tubes






3606 xg





Items included: 30 Tube holders

30 Wells for round bottom tubes

Accessories for NU-RA30-15

Cups

Part Number: NU-3015R

Description: 15 ml round-bottomed tube cups (Set of 30)

Maximum radius : 138 mm (with NU-3015S)

Part Number: NU-3015C

Description: Tubes for 15 ml tubes with conical bottom (Set of 30)

Maximum radius : 137 mm (with NU-3015S)

Tubes

Part Number: NU-3015S

Description: Tube holder for 15 ml round bottom tubes (Set of 30)

Maximum radius : 140 mm (without cups)

Configurations :

Loading the rotor

The rotor must be loaded evenly.

Allowed

Forbidden: A load with number of odd tubes is prohibited for this rotor.


9.7. Angle Rotor NU-RA16-5 Rotor

(Lid included)









21000 xg




Maximum radius 107.8 mm

Items included: Micro tube rotor airtight lid

Accessories for NU-RA16-5

Lid

Part number: NU-LM2

Description: Micro tube rotor airtight lid for rotors NU-RA30-2, NU-RA48-2 and NU-RA16-5

Configurations:

Loading the rotor

Allowed

Forbidden:



Rotor

(Lid included)









26620 xg




Maximum radius 99 mm (outer row) 89 mm (inner row)

Items included: Micro tube rotor airtight lid for rotors NU-RA30-2 and NU-RA48-2

Accessories

Lid

Part number: NU-LM2


Configurations:

Loading the rotor

Allowed :

Forbidden:



Rotor

(Lid included)









27720 xg





Items included: Airtight lid for rotors NU-RA30-2 and NU-RA48-2

Accessories

Lid

Part number: NU-LM2


Configurations:

Loading the rotor

Allowed

Forbidden:


10. EC Conformity Declaration


Blank page.

Come and discover our

different ranges of

centrifuges.

3, rue Nicolas Copernic

ZA Nord Bazouges

53200 CHATEAU-GONTIER

FRANCE

Tél : +33(0)2 43 06 66 76

contact@ afigroups.com

www.aficentrifuge.com Non-contractual photos.

All rights reserved, including photos and illustrations.

Copyright ® AFI Centrifuge company – SIRET 513 982 645 00022.

Documents

EN - LOREENA User Manual - Rev1.3.pdf - AFI Centrifuges