World Precision Instruments

2007

World Precision Instruments

Laboratory Equipmentfor the Life Sciences

Micro-manipulators

Physiology—Cardiovascular

Biosensing

Microdissection,Microsurgery

Microscopes,Cameras

Spectroscopy, Fluorometry

Physiology—Epithelial

Index

Neuroscience

Pumps, Fluid Handling

Laboratory Supplies

Physiology—Miscellaneous

Glass, Holders,Electrodes

Free RadicalAnalyzers & Sensors

See page 86

Data Acquisition Hardware & Software

See page 70

INTERNATIONAL EDITION

The colorful winner of WPI’s 2006 Images of Science contest was submitted by Dr. Tim Murphy of University of British Columbia Department of Psychiatry in Vancouver, B.C., Canada.

Dr. Murphy is a basic research scientist using sophisticated, two-photon microscopy techniques to look at individual synapses and brain capillaries in mouse models of stroke in vivo.

The winning image was created in one of Canada's first two-photon microscope labs. According to the Brain Research Centre Newsletter, the first largescale two-photon imaging facility in Canada capable of high resolution in vivo investigations of disease states recently entered service at UBC. Funding awarded last year to Tim Murphy and four other researchers enabled building the facility,

which houses four two-photon microscopes, allowing investigators to resolve structures down to one-thousandth of a millimetre in the living brain. This makes it possible for investigators to view individual synapses and provides new tools to investigate models of human neurological diseases and stroke.

Dr. Murphy is also listed as a faculty member of the International 3D Microscopy of Living Cells Course, next summer at UBC.

For submitting the winning entry in WPI’s Images of Science Contest, Dr. Murphy received an Apple iPod.

Images of Science

Brain blood vessels and synapses in a living mouse, made by two-photon imaging—a maximal intensity projection of the first 100 microns of mouse (YFP-H transgenic line) somatosensory cortex. The small vessels are brain capillaries and are 3-5 microns in diameter. The image area is 139 microns wide. The animal was prepared using WPI surgical instruments and Novaflex fiber optic illuminator. Recordings of EEG activity were made using WPI silver wire and DAM50 amplifiers. For more information see: J Neurosci. 2005 Jun 1;25(22):5333-8.

Two-photon microscopy

Dr Timothy H. Murphy

Keep in touch ...

— visit WPI on-line!Need to request a quote? Looking for an instruction manual? Looking for references?

In Europe, point your Web browser to www.wpi-europe.com

In the rest of the world, go to www.wpiinc.com

http://www.wpiinc.com

Glass, Holders, Electrodes . . . . . . . 179Glass, Holders, Electrodes . . . . . . . 179

Neuroscience . . . . . . . . . . . . . . . . . . . . . . . . . 2

Physiology — Cardiovascular . . . . . . . . . . . . 35

Physiology — Miscellaneous . . . . . . . 64

Biosensing . . . . . . . . . . . . . . . . . . . . . . . .79

Lab Supplies . . . . . . . . . . . . . . . . . . . . . 165

Microdissection, Microsurgery . . . . . .102

Micromanipulators, Stands . . . . 192

Pumps, Fluid Handling . . . . . . . . . . 222

Physiology — Epithelial . . . . . . . . . . . . . . . . . . . . . . 54

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265 OrderingInformation,Terms&Conditions . . . . . . . . . . . . . . . .272

Microscopes, Cameras . . . . . . 208

Spectroscopy . . . . . . . . . . . . . . . . . . . . . . . . . . 242

Amplifers,StimulatorsandIsolatorslStereotaxicInstrumentsl Microforges,BevelersandPullers .

BloodPressureMeasurementlForceTransducerslTissue&OrganBathlBridgeAmplifierlModularTransduceramplifier

Voltage/CurrentClamplEpithelialVoltohmmeterlTEERMeasurementlUssingSystem

AnimalTemperatureControllerlMicrotomeslDataAcquisitionlMicropressureSystem .

NitricOxideDetectionlIonSelectiveElectrodeslOxygenDetectionlpHmeters

ScissorslTweezerslEarPuncheslVesselClipslMouseSurgeryKitlElectrosurgicalUnitlCauteryInstruments

AdhesiveslCables&ConnectorslMetalWirelLuers&FittingslLaboratoryBases,Rods,andClamps

CapillaryGlasslElectrodeHolderslMetalMicroelectrodeslMicroFilNon-metallicSyringeNeedles

Manual&MotorizedMicropositionerslMagneticStandslTiltingBase

SpectrometerslSampleCellslLightSourceslFiberOpticCableslFiberOpticDippingProbe

Peristaltic,Syringe,andPneumaticPumpslMicroprocessorControlledInjector

Surgical,Stereo,andInvertedMicroscopeslCameraslIlluminatorslHeaterControllerlCultureDishes

Laboratory Equipment 2007

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NeuroscienceIntracellular Amplifiers

Electro 705Electrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Duo 773DualMicroprobeSystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Extracellular AmplifiersDAM50 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

DAM80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

ISO-80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

ISO-DAM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Miscellaneous Neuroscience Instruments121WindowDiscriminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

260DualMicrointophoresisCurrentGenerator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Stimulators & IsolatorsDS80008-ChannelDigitalStimulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

DLS100DigitalLinearStimulusIsolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

A310Accupulser™SignalGenerator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

A300Plusemaster™Multi-ChannelStimulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

A320Isostim™Stimulator/Isolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

A362BatteryCharger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

A360LAHighVoltageStimulusIsolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

A365HighVoltageStimulusIsolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

A385HighCurrentStimulusIsolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

A382BatteryCharger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

A395LinearStimulusIsolator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Stereotaxic Instruments & AccessoriesStereotaxicInstruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Mouse&NeonatalRatAdaptorforStereotaxicInstruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

DigitalStereotaxicFrame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

MetalMicroelectrodes—Tungsten,Iridium,Platinum-Iridium,Elgiloy® . . . . . . . . . . . . . . . . . . . . . . . . . 24

Ωmega-Tip-ZMillivoltandMegohmMeter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

RodentBrainMatrices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Microforges, Bevelers, PullersDMF1000Microforge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

H6021000xCompoundMicroscopeforDMF1000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

MF200Microforge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

48000Microbeveler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

1300MMicroelectrodeBeveler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

PMP-107ProgrammableMultipipettePuller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

PUL-2Programmable2-StageMicropipettePuller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Prices shown are in U.S. dollars. Actual charges will vary because of import duty, freight, and currency fluctuations. To obtain an exact quotation, contact your WPI office.

UK: Tel: 01438-880025•Fax: 01438-880026•wpiuk@wpi-europe .comGermany: Tel: 030-6188845•Fax:030-6188670•wpide@wpi-europe .com

Mailto:[email protected]


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Electro 705Electro 705

A low noise high quality intracellular amplifier well-suited for the student lab

●Remote Headstage—Easilymountedinanymanipulator,thissmallprobe,containingthefirststageofamplification,includesamicroelectrodeholder,whichplugsdirectlyintotheprobeinput .

●Battery Power—Four9Valkalinebatteries(included)powertheElectro705forapproximately500hoursgivingasupercleanlownoisesourceofpowermakingtheElectro705thequietestamplifieravailable .Batteriescanbeeasilytestedbythepressofabutton .

●Capacitance Compensation—Correctsforlossofrisetimecausedbythepresenceofelectrodecapacity .Upto50pFofelectrodeshuntcapacitymaybeneutralized .

●Driven Guard Shield—Straycapacitancecanbefurtherreducedbyplacingthedrivenguardshield(included)overthemicroelectrodeholderattheinputendoftheprobe .

●Tickler Circuit—Amomentaryoscillationthathelpsachievecellpenetration .

●Electrode Resistance Test—The705providesa1nAelectrodetestcurrent .Electroderesistanceismonitoredatthe1Xoutputasavoltage(1mV/M) .

●Probe Test Port—Allowstheconvenienceoftestingtheamplifier'sintrinsicnoiseandgainwithoutcumbersomeexternaltesthookups .Gateleakagecurrentcanalsobeadjustedwithminimumeffort .

●Baseline Position Control —Addsorsubtractsupto300mVtotheheadstageoutput,allowingartifactvoltagessuchasliquidjunctionpotentialstobenulledpriortorecording .

●Differential Output—TwoElectro705scanbeconnectedintandemtocreateanoptionaldifferentialamplifierprobesystem .

BATTERYPOWERED

BATTERYPOWERED PORTABLE

PORTABLE

WPI’s Quietest Intracellular Amplifier!

ELECTRO 705 SPECIFICATIONSINPUTIMPEDANCE 1012Ohms,shuntedby1pF

OUTPUTIMPEDANCE 100Ohms,bothoutputs

GAIN X1:±0 .1%

INPUTVOLTAGERANGE ±5V

RISETIME 15µs,10-90%

NOISELEVEL 500µVpeak-to-peak*

INPUTCAPACITANCECOMPENSATION 0-50pF

GATELEAKAGECURRENT ±10pA,adjustabletozero

ELECTRODERESISTANCETEST 1mV/MOhms

DCPOSITIONING ±300mV

COMMONMODEREjECTION >104(indifferentialmode)

POWER Four9Valkalinebatteries,supplied

DIMENSIONS 8 .5x3 .5x2 .2in .(22x9x6cm)

SHIPPINGWEIGHT 5lb(2 .3kg)

* Full band width, with 20 M Ohms sourceReferenceKoch, U . (2000)“Interdependenceofspatialandtemporalcodingintheauditorymidbrain .”Journal of Neurophysiology83,4,2300-2314

Photo shows two units arranged for differential recording. Manipulators not included.


World Precision Instruments • Tel:941-371-1003 • Fax:941-377-5428 • E-mail:sales@wpiinc .com • Internet: www.wpiinc.com

705 Electro705ElectrometerProbe, driven guard shield and micropipette holder MEH1SF included for

glass microelectrodes O.D. 1.0 mm, 1.2 mm, 1.5 mm, or 2.0 mm.

OPTIONAL ACCESSORIES3468 DualRackMountKit3469 SingleRackMountKitM3301L Micromanipulator(specify left- or right-handed)M-3 80°TiltingbaseRC1T Referencecell(Ag/AgCl)2541 Drivenguardshieldfor705PFProbeMEH1SF Microelectrodeholder705PF Replacementprobe(includescalibration)*

*Instrument must be returned to WPI for free calibration with new probe.

See cables and connectors, page 184.See microelectrode holders, pages 34-37.

See capillary glass, page 29.

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Duo 773 Dual Microprobe System2-channel intracellular amplifier

DUO 773 SPECIFICATIONSHEADSTAGE (PROBE) 712P (red, port “B”) 715P (blue, port “A”)ACTIVEPROBEINPUTIMPEDANCE >1011Ω 1015ΩGAIN x1,x10 x1OUTPUTRESISTANCE 100Ω 100ΩOUTPUTVOLTAGERANGE ±10V ±10VMAXIMUMINPUTVOLTAGE ±15V ±15VPROBELEAKAGECURRENT 5X10-12A 10-14ADCPOSITIONADjUSTRANGE ±300mV ±300mVELECTRODERESISTANCETESTCURRENT 1nA 1pA,1nAselectableINPUTCAPACITYCOMPENSATION +10to-50pF 0to-10pFNOISE Inputshorted <50µVp-p10kHzbandwidth <50µVp-p10kHzbandwidth 20MΩcarbonresistor <200µVp-p10kHzbandwidth <200µVp-p10kHzbandwidthRISETIME 10-90%directinputsmallsignal 1µs,typical 10-90%through20MΩ(-C“on”) 25µs,typicalCURRENTINjECTION (712Ponly)** InternalDCCurrent ±50nAlowrange,±500nAhighrange ExternallycommandedCurrent ±500nAlowrange,±5µAhighrange Externalcurrentcommandfactor 20mV/nAlowrange,2mV/nAhighrange Currentmonitor 100mV/nAlowrange,10mV/nAhighrange Compliance 3Vlowrange,10Vhighrange Bridgebalance 0-100MΩ,0-1000MΩ Bridgeamplifiergain x10,x50LOWPASSFILTER 40dB/decade,continuouslyvariable1-30kHzMETERSECTION Display 3 .5-digitLED Ranges 200mV,2000mV,20V,200nA,2000nA Accuracyandresolution 1digitDIMENSIONS Instrument 17x5 .25x10in .(43x13x25cm) Probe Diameter:12mmLength:34mmPOWER 95-135Vor220-240V,50/60HzSHIPPINGWEIGHT 15lb(7kg)CERTIFICATION CE,CSA

* Although injected currents are “constant,” the maximum current in a given situation will always be limited by the system compliance of 10 V.**The 712P headstage may be used on either A or B channels, however Current Injection specifications do not apply when used on channel A. The 715P headstage may not be used on the B channel.

ReferencesL. Pluja (2000)“Electricalandmechanicaleffectsofvasoactiveintestinalpeptideandpituitaryadenylatecyclase-activatingpeptideintheratcoloninvolvedifferentmechanisms .”European Journal of Pharmacology389,217-224 .

G. X. Wang, X. B. Zhou, et al. (2000)“Effectsofmitoxantroneonexcitation-contractioncouplinginguineapigventricularmyocytes .”Journal of Pharmacology and Experimental Therapeutics 293,2,501-508 .

S. Tsuruoka(2000)“Acuteeffectofcadmium-metallothioneinonglucoseandaminoacidtransportacrosstheapicalmembraneoftherabbitproximaltubuleperfusedin vitro .”Journal of Pharmacology and Experimental Therapeutics 292,2,769-777.

For intracellular dual or differential studies, the Duo773 has separate negative capacity controls and built-in active filtering that allows the precise balancing of time constants for artifact-free differential measurement. Comes complete with two probe headstages, 1015 Ohms & 1011 Ohms probes to monitor signals from ion-specific micro-electrodes as well as KCl-filled electrodes.





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Duo773, Intra 767, Cyto 721

Inverted Microscope

Micromanipulator

Recording Electrode (with holder MEH1SF)

Drug or dye current electrophoresis injection

50x gain output

Micromanipulator

Data Acquisition

System

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#2547 Driven Guard Shield

Headstage—Twogold-plated,epoxysealedminiatureactiveprobescanbepositioneddirectlytothemeasurementsite .MicroelectrodeholderscontaininganAg/AgClelectrochemicalhalf-cellsplugdirectlyintotheprobes .Straycapacitancecanbereducedbyplacingtheincludeddrivenguardshieldoverthemicroelectrodeholderattheendoftheprobe .

Capacity Compensation—ChannelAcancompensateupto10pFofelectrodeshuntcapacityandChannelBcancompensateupto50pF .

Tickler Circuit—Assistsincellpenetration .Thefrequencyandamplitudeoftheoscillationsmaybevariedfordifferencesinmembranethicknessorcellsize .Thedurationofticklecanbecontrolledeitherbyusingthemomentaryswitch,afootswitch,orbyapplyingasignaltotheremoteticklerinput .

Active Filters—Lowpasssettingsona-40dB/decadeactivefiltervarythecutofffrom1to30kHz .Eitherprobeorbridgeoutputsmaybeselectedforfiltering .

Current Injection—ChannelBcanejectcurrentthroughthemicroelectrodebyapplyingacommandsignaltothestimulusinputconnector;theresultingoutputfromtheprobewillthenbeaconstantcurrentreplicaoftheinputsignal .Tworangesofcurrentdeliveryareprovided:50nAand500nAorbyanexternalsource .Thissourcecanbeusefulfordeliveringhyperpolarizingcurrentstostabilizethecellmembranepotentialandasaholdingcurrentformicroiontophoresis .

Compliance Alarm—Whentheelectrodevoltageexceedstheprobeinputmaximumallowedvoltage,anaudibleover-compliancealarmwillsound .

Bridge Balance—Subtractstheexcesselectrodevoltageassociatedwithdeliveringcurrentthroughtherecordingmicropipette .Electroderesistancesupto1000MΩcanbebalancedintworanges .Thebalancedsignalisavailablefromx10orx50frontpaneloutputconnectors .

Independent Outputs—TheDuo773hasanoutputforeachprobeindependentofgainfilteringorbalancing .InadditiontheDuo773hasa10xanda50xoutputforeasyintegrationtomostdataaquisitionprograms .

Digital Meter—TheDuo773comescompletewitha3½-digitdisplayformonitoringinjectioncurrentorthevoltagesforeitherprobe(singleendedordifferential) .

Typical setup:

MEH1SF (supplied)

MEH2SFW

MEH3SF

MEH3SFW

MEH6SF

MEH6SFW

MEH2SF

Optional Holders for Intracellular

Amplifiers

See Microelectrode Holders,



773 Duo773ElectrometerSpecify line voltage

Includes two probes with driven guard shields and eight MEH1SF microelectrode holders for 1.0 mm, 1.2 mm, 1.5 mm,

or 2.0 mm glass electrodes.

OPTIONAL ACCESSORIES712P Replacementprobe(includescalibration)*715P Replacementprobe(includescalibration)*

*Instrument should be returned to WPI for free calibration with new probe.

2933 RackMountKit,51⁄4-in .high2547 DrivenGuardShieldfor712P&715PProbes15790 ReplacementProbeHandleTW100F-4 GlasscapillarywithfilamentTW150F-4 Glasscapillarywithfilament

See Dri-Ref, page 112.See cables and connectors, page 184.

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DAM50, DAM60, DAM70

#5447 AdapterGND GND

Electrode Adapter

A B

Metal Microelectrodes

#300102 electrode extension, held by micromanipulators

Immobilized specimenCentral Ground

LightStimulation

#5371 Cable

#5371 Cable

#3578 Cable

Ag/AgCl pellet

DAM50, DAM60, DAM70

Micromanipulator

#5489 Probe

#2033 Adapter

#5469 MetalMicroelectrode

Adapter

Metal Microelectrode(i.e., TM33B01)

EP2 Ag/AgCl Half Cell

Petri Dish

AB

Central Ground

#3294 Grounding Clip

•Gatedormanualcurrentgenerationforhistologicalmarking,iontophoresis,orcellstimulation .

•Stimulusartifactsuppression(SAS)circuitrymitigatesamplifierinputstageoverloadfromstimulustransients

bydramaticallyreducingtheheadstagegainwhenastimulationpulseisapplied .Thisresultsinquickerrecoveryoftheinputtoamplifiytheensuingbio-potentials .TheSAScircuitryisinvokedviaastandardTTLcontrolsignal .

•Anverylownoiseremoteactiveheadstage(DAM80only)isusefulforveryhighimpedanceamplificationutilizingglassormetalelectrodes .

DAMseriesamplifierscanbeusedasstandaloneunitsonanytabletop,oruseoptionalclamp-mountinghardwaretolocatethemconvenientlywithintheworkarea .Alternatively,apairofamplifierscanbemountedintoastandardequipmentrackwitharackmountkit(#3484) .Avarietyofhookupaccessoriesareavailabletoconfigureyourapplication .

WPI’sDAMseriesamplifier’sarewellknownasastandardoftheindustryforextracellularpotentialamplification .Thesebatterypoweredbio-amplifiersaredesignedwithacompactchassisprofilethatenablestheusertolocatetheunitclosertothepreparationandtherebyminimizelongleadlengthswhichcontributetonoise .Eachamplifierisequippedwithselectablehighandlowfilters,andapositioncontroltooffsetgalvanicpotentialswhichmaydevelopduringrecording .Achoiceofmodelsofferadditionalfeaturesthatareusefulforcertainapplications:

DAM Series BioamplifiersA family of very low noise battery-operated amplifiers

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ERG recording of fly eyes

Application Examples

Dam50—Basic Amplifier (optional #5447 electrode adapter not included)

Extracellular recording using metal microelectrode

Optional probe #5489 for use with DAM50 also includes microelectrode adapter #5469 .

Now with ESD Protection!





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#13388 Adapter

#5371 Cable

DAM80 or ISO-80 DAM80i Probe (included)

#3294 Grounding Clip (included)

Ag/AgCl skin electrode pad(#EL203)

AB

Metal Microelectrodes

#300102 electrode extension, held by micromanipulators

DAM SERIES SPECIFICATIONSINPUTIMPEDANCE 1012Ω,commonmodeanddifferential

INPUTLEAKAGECURRENT 50pA(typical)

MAX .DCDIFFERENTIALSIGNAL ±2 .5V(DAM50)

GAIN AC:100x,1000x,10000x DC:10x,100x,1000x(DAM50)

COMMONMODEREjECTIONRATIO 100dB@50/60Hz

INPUTCAPACITANCE 20pF

ACMODENOISE 0 .4µVRMS(2µVp-p)0 .1-100HzACMODENOISE 2 .6µVRMS(10µVp-p)1Hz-10kHDCMODENOISE(DAM50) 7 .5µVRMS(30µVp-p)3-10kHz

BANDWIDTHFILTERSETTINGS

ACMode Lowfrequency,0 .1,1,10,300Hz ACMode(DAM80) Highfrequency,0 .1,1,3,10kHz DCMode(DAM50) Highfrequency,0 .1,1,3,10kHz

OUTPUTCONNECTORS BNConDAM50;3 .5mmMiniPhone connectoronDAM80

OUTPUTVOLTAGESWING ±8V

OUTPUTIMPEDANCE 470ΩBATTERYTEST Audibletone

CALIBRATORSIGNAL 10Hzsquarewave

POSITION Approximately250mV

CURRENTSOURCE

DAM80:DCGenerator 0to±50µA,variable

EXTERNALCOMMAND InputVoltage±10Vcommands

ACORDCCURRENTWAVEFORM ±50µAmax .amplitude@200KΩBATTERIES 2x9Valkaline(included)

DIMENSIONS

DAM50 8x4x1 .75in .(20 .3x10 .2x4 .4cm) DAM80 7x4x1 .75in .(17 .8x10 .2x4 .4cm)

SHIPPINGWEIGHT 3 .5lb(1 .6kg)

Dam80—With low-noise headstage DAM80P

Metal microelec-trode adapter #5469 included .

DAM Series amplifiers — and most other

small WPI instruments with similar metal

enclosures — may be mounted on a post

near the experimental site with Ringstand

Mounting Kit (#3485)

DAM80,anACamplifieronly,featuresaverylownoiseheadstageprobewhichcanbemountedinmicromanipulatorsforup-closecorticalrecording,forextracellularrecordingfromhighimpedanceglassormetalmicroelectrodes .Alsoprovidesagatedcurrentfortissuemarking .Micro-electrodeholderMEH3SBisrecommended .

FEATURE DAM50 DAM80InputMode AC/DC ACInputconfiguration differential/singleended differentialGainRange 100-10K(AC),10-1K(DC) 100-10K(AC)High/LowFilters yes yesOffsetpositioncontrol yes yesCurrentGenerator No YesRemoteActiveheadstage No YesOutputconnection BNC 3 .5mmminiphoneStandardinputconnection* unterminatedwire minibananaPowersupply (2)ninevoltalkalinebatteries (2)ninevoltalkalinebatteries

*seeoptionalaccessoriesforadditionalalternatives

ERG recording of a rat



3484 RackMountKit(for2DAMpreamps)3485 RingstandMountingKit5447 ElectrodeAdapter(DAM50)5469 MetalMicroelectrodeAdapterforDAM80

(mini-banana plug to 0.031 in. (0.79 mm) socket)5489 AdapterforMetalMicroelectrode(DAM50)13388 Adapter,mini-bananaplugto2mmsocket5371 Cable,LowNoise(2mmpinto2mmpin)3578 AdapterCableforAg/AgClpellets(2mmpin)300102 ElectrodeExtension,4-inch3414 9VNiMHBattery

Also see cables and connectors, metal microelectrodes, carbon-filled micropipettes.

DAM50Bio-amplifierDAM80 Bio-amplifierwithactiveprobe(DAM80P)

OPTIONAL ACCESSORIESDAM80P ReplacementProbe3072 6ReplacementModularCables(DAM50)3517 2OptionalShieldedModularCables(DAM50)CBL102 3 .5mmPhoneplug-to-BNCCable2851 BNC-to-BNCCable2033 BlackInsulatedMini-BananaPlug2034 RedInsulatedMini-BananaPlug2035 UninsulatedMini-BananaPlug2101 9VAlkalineBattery,each(2required)

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Isolated Differential

Amplifier

ISO-80 SPECIFICATIONSINPUTRESISTANCE >1011Ohms,CommonModeanddifferential

InPuT LEAkAGE CuRREnT 50 picoamperes, max.

AMPLIfICATIOn ×102,×103,×104

COMMONMODEREjECTIONRATIO 100dBtyp .@50/60Hz

EqUIVALENTNOISESIGNALINPUT 0 .4microvoltsrms(0 .1-100Hz) 2 .0microvoltsrms(1Hz-10kHz)

FILTERSETTINGS Lowfrequency 5,10,100,300Hz Highfrequency 100Hz,1,3,10kHz

MAX .OUTPUTVOLTAGESWING ±8volts

ELECTRODEIMPEDANCERANGE 100kOhm-10MOhm@300Hz

STIMULATIONCURRENT 0to±20microamperes(constantcurrent)

MAXIMUMSTIMULATIONVOLTAGE ±15volts

MAXIMUMELECTRODEVOLTAGE ±40volts

DISPLAY 31⁄2-digitLCD

BATTERYTEST Lowbatterydisplay

POWER Two9-voltNiCadbatteries&charger,supplied


TheISO-80 Startup kitnowcontainsallthebelowlistedaccessoriesneededforbasicmetalelectrodeelectrophysiologyresearch:

CBL102Cable,BNC-to-3 .5mmplug,6ft(2m)(one)

5469Adapter,mini-bananato0 .031skt .(two)

13388Adapter,mini-bananato2mmskt .(two)

3294Cable,groundcliptowire,3ft

2033Mini-bananaplug,black

2034Mini-bananaplug,red

2035Mini-bananaplugsolderableturrent(two)

EP1Ag/AgClpellet(70mmwire)1mmdiamx2 .5mmlong

M3301EHElectrodeHolder,14cm(two)

54700 .031-inchjackon12-inchwire(packageof4)

TheimprovedISO-80provideslownoiseACcoupledamplificationandoffersexcellentrecordingperformanceformonitoringextracellularnerveactionpotentialsin vitroandinlivinganimals .TheISO-80isprovidedwitharemoteheadstage(1mcable)whichincorporatesanelectrodeimpedancetestfunctionandaconstantcurrentstimulator .Theconstantcurrentstimulatorcanbeusedforcellmarking,stimulationorelectrodecleaning .TypicalapplicationsincludemeasuringEMG,EEG,extracellularandactionpotentialsin vitroorin vivo .TheISO-80systemisDCisolatedfromthesubjectgroundandemploysstateoftheartelectro-magneticshieldingforimprovednoiserejection .Theamplifieremploysbothhighpassandlowpassfilteringwithgainfrom100to10,000 .Thelowestlow-passsettingis5Hzandtheupperpassbandis10kHz .

To ISO-80

micro-manipulator

ISO-80 probe

#5469 electrode adapters*

metal microelectrodes

specimen

EP1* or similarAg/AgCl half-cell

#3294 grounding wire & clip*

To ISO-80

micro-manipulator

ISO-80 probe

#5469 electrode adapter*

#2033connector*

metal microelectrode

specimen

red socket


#3294 grounding wire & clip*Cut, strip, and secure the wires in #2033 connector.

To ISO-80

micro-manipulator

micro-manipulator

ISO-80 probe*

#5469electrodeadapter*

#5470electrodeadapter*

#M3301electrodeholder*

metalmicroelectrode

redsocket specimen


#3294 grounding wire & clip*

#2033 connector*

Differential Application Single-Ended Application Optional Differential Application



ISO-80 IsolatedBioamplifierw/activeprobe(ISO80P)

Specify line voltage

OPTIONAL ACCESSORIESISO-80P ReplacementISO-80ProbeCBL102 3 .5mmphoneplug-to-BNCcable



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Up to four unitsmay be daisy-chained using

a single AC adapter with power jumper cable(s) (#13246).

Iso-DAMisopticallyisolatedsonosignificantcurrentcanflowfrominputterminalsofthepreamplifiertoground .Theinstrumentisintrinsicallysafeandcannotcauseanyelectricalstimulusorshocktothesubject .Noisefromgroundloopsiseliminated .Iso-DAM’ssmall-boxstructureallowstheinstrumenttobeplacedclosetothesiteofmeasurement .ThefrontstageofthisdeviceisbatterypoweredbysixAANimhbatteries(included) .Runningtimeafterfullchargingisaboutsevendays .Powerfortheoutputstageissuppliedbyacompactwallpowerunit,whichalsochargestheNi-Cdbatterieswhentheamplifierisnotinuse .Uptofourunitsmaybedaisy-chainedwithPowerjumperCable(Part#13246),drawingpowerfromasingleACadapter .Iso-DAMscanbeconfiguredingroupsoffourinaconvenientrackMount .

Iso-DAM

metal microelectrode

#300102 electrode extension, held by micromanipulator

immobilizedinsect antennae

Central Ground

solderconnection

3-pin connectorcable (included)

Application Example: insect antennae recording

Iso-DAMIsolated Low Noise Bio-Amplifier

ISO-DAM SPECIFICATIONSVOLTAGEGAIN x10,x100,x1000,x10,000

INPUTIMPEDANCE >1012Ohms,nominal

AMPNOISE,INPUTSHORTED 0 .2µVRMS(1-100Hz) 1µvRMS(1-10KHz)

BANDWIDTH DCto10kHz Filter(lowpass) 0 .1,0 .2,0 .5,1,3,10kHz Filter(highpass) 0 .1,1,10,300Hz

INPUTOFFSETVOLTAGEADjUST ±100mV

COMMONMODEREjECTION >100dB@50/60Hz

OUTPUTVOLTAGESWING ±5Volts

OUTPUTRESISTANCE 270Ohms

DIMENSIONS 8×4×1 .75in .(20×10×4cm)


BACK BY POPULAR DEMAND!



OPTIONAL ACCESSORIES3545 120VACAdapter3546 240VACAdapter(Euro)3547 240VACAdapter(UK)13246 PowerjumperCable2851 BNC-to-BNCCable13451 Adapter,two2mmpinstoBNC(F)5381 RackMount(willmountupto4units)15997 ReplacementIso-DAMcable13893 Replacement3-pinconnector

COMPLETE SYSTEMISO-DAMX LowNoisePreamplifierwithpowersupplyISO-DAM LowNoisePreamplifierwithoutpowersupply


Both preamplifiers include input cable, one power jumper cable, miniature ground plug and 10’ BNC cable.

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This amplitude discriminator is designed for audio and sub-audio signals such as extracellular nerve action potentials. For every waveform peak that appears “within” the window aperture set by the user, a pulse is generated at the “Within Window” output. Signals that exceed the upper level of the window produce a pulse at the “Above Window” output. A visual indication of the pulses is provided at each output by the LED display. Both the input signal and the window aperture settings are visible at the multiplex port as shown below in the upper trace. By viewing the input signal as well as the “window” discriminator levels at the multiplex port provides a convenient visualization and ease in setting up an experiment. Model 121’s multiplex circuitry samples input signals and window levels on a 70%/30% basis respectively, minimizing loss of signal information. The Lower Level control sets the lower discrimination level, and the Window Aperture control sets the upper window level with respect to the lower level setting. This means that changing the lower level setting has no effect on the window aperture except to displace the window up or down. It is impossible to set the lower level higher than the upper level, a feature unique to Model 121. The pulses from either

MULTIPLEX OUTPUTS

The diagram above shows the multiplex signal output with the widow parameters displayed on the input signal. A “within” pulse occurs at the lower level crossing point after the input signal is measured within the boundaries of the settings and an “above” pulse is generated for those signals going above the upper level setting.

output (Within Window or Above Window) are generated by the falling edge of the analyzed signal as it crosses the preset levels. Because the logic circuit is not time dependent, Model 121 can be used to analyze signals of any duration. Ten-turn controls with dial indicators allow precise setting and direct reading of the window levels.

Enclosure includes a tilt stand for bench operation. Rack mounting hardware is optional.

WINDOW DISCRIMINATOR SPECIFICATIONSInpuT IMpEDAncE 100 K Ohms

MAxIMuM FREquEncy Approximately 10KHz

MInIMuM puLSE WIDTH 80 µs

InpuT pOLARITy positive or negative switchable

MAxIMuM InpuT VOLTAgE up to 50 V (peak-to-peak) in three ranges: 0-0.5 V, 0-5 V, 0-50 V

WInDOW RAngE uppER, LOWER LEVELS up to 10 V in three ranges: 0-0.1 V, 0-1 V, and 0-10 V

DIScRIMInATOR RESOLuTIOn 0.1% of full scale (selected range)

DIScRIMInATOR AccuRAcy ±2%

WITHIn & ABOVE WInDOW OuTpuT Rectangular pulses, approx. 3.0 V amplitude (TTL compatible), adjustable pulse width from 0.1 to 1.0ms. Output impedance less than 500 Ohms. LED indicates presence of output pulse. cycle length nominally 37 µs.

MuLTIpLEx OuTpuT Signal on time: 26 µs (70%) Levels on time: 11 µs (30%) Output impedance less than 100 ΩpOWER REquIREMEnTS 95-135 V or 220-240 V, 50/60 Hz, 10 VA

DIMEnSIOnS 17 x 3.5 x 9.5 in. (43.2 x 8.9 x 24 cm)

SHIppIng WEIgHT 11 lb (5 kg)

Window Discriminator● Monitor signals and discriminator levels simultaneously at the multiplex output● Window height independent of lower discriminator level setting● Logic level output pulses, TTL compatible● Output pulses indicated by LED display

Upper Level

Lower Level

Input Signal

Window Output

Above WindowOutput


UK: Tel: 01438-880025 • Fax: 01438-880026 • [email protected] Germany: Tel: 030-6188845 • Fax: 030-6188670 • [email protected]

121 Window Discriminator 2932 Rack Mount Kit, 3.5-in. High2851 Bnc-to-Bnc cable, 6 ft500184 Bnc-to-Bnc cable, 10 ft500257 Bnc-to-Bnc cable, 6 inch 15 cm500258 Bnc-to-Bnc cable, 12 inch 30 cm500259 Bnc-to-Bnc cable, 18 inch 46 cm



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Dual Microiontophoresis Current Generator

TheDualMicroiontophoresisCurrentGenerator(Model260)isanelectricallyisolated,battery-operatedinstrumentdesignedfortheelectro-iontophoresisofdyes,drugsandchargedsubstancesfrommicropipettes .Twoidenticalbatteryoperatedcurrentgeneratorsareavailable .Inordinaryuse,thetwocurrentgeneratorsareoperatedinparallelprovidingtwodistinctcurrents;oneforpreventingsubstancesinthemicropipettefromoutwarddiffusion(theretainorholdcurrent)andthesecondforactivelyejectingchargedmaterial .Forpipetteswithsubmicrontips,aholdcurrentmaynotbenecessaryifthereislittleoutwarddiffusionofpipettematerial .Model260ispoweredbytwo9-voltalkalinebatteriesperside(four,intotal);uniquecircuitryconvertsthe±9Vto±100Vwithoutatransformer,yieldinganexceedinglyquietoutput .

Single Channel OperationModel260containstwocompletelyindependent,batteryoperatedcurrentgeneratorswhichmaybeoperatedindependentlyorinparallelconnection .Theuserneedonlyconnecthisexternalelectrodepairtotheredandblackoutputterminalsofchannel1(HOLD)or2(EjECT) .Forcurrentsbelow100nanoamperesthe100nanoampererangeisrecommendedbecauseonemayadjusttheselowcurrentvalueswithbetterresolutioninthelowercurrentrange .

Two Channel Operation, Hold-EjectTheelectrophoreticreleaseofchargedsubstancesfrommicropipettesoftenrequiresasecondcurrenttocounteroutwarddiffusionofmaterialfromthedeliverypipette .Thiscurrenthasbeenreferredtointheliteratureas“backing,holdingorretaining”current .Operationcausesasmallcurrenttoflowinadirectionthatwilloffsetthespontaneousleakageoftheactiveagentfromthepipetteuntilitisrequired .

AUTO OperationThetimingofcurrentflowfromeachchannelofthemodel260canbeexternallycontrolledbyapulsegeneratororcomputerthatcansupplyalogiclevelofatleast+5voltstotheAUTOINPUTterminal .Uponapplicationofthe+5voltcommandpotentialtotheAUTOINPUTterminal,thepresetcurrentwillflowfromtheoutputterminalsforaslongasthecommandpotentialismaintained .Note:IftheinstrumentisintheMANUALmode,theapplicationoftheAUTOINPUTsignalwillhaltthecurrentforaslongasthiscommandismaintained .ThisisthecomplementofnormalAUTOoperation .

Compliance AlarmsEachcurrentgeneratorchannelcontainsanaudibleandvisualalarmcircuitthatwarnswhenthecurrentamplitudeislargeenoughtoexceedthecompliancevoltage;inotherwords,theloadresistanceistoohighfortheamountofcurrentflowing .Theuseristhusinformedthattheinstrumentcannotdelivertheamountofcurrentindicatedonthepanelammeter .Thealarmwillalsobetriggerediftheoutputpathisopen-circuitedbyalooseconnection,airbubbleorablockedmicropipette .Theinitialcompliancevoltageexceeds100voltsbutwilldecreaseproportionatelyasthebatteryvoltagefallswithage .

260 SPECIFICATIONSOUTPUT Constantcurrent,floating

OUTPUTRANGES 0to±100nA 0to±1000nA

OUTPUTPOLARITY Switchselected

COMPLIANCE(EXCURSION)VOLTAGE 100V

METER Analogmeters,±2%accuracy

OUTPUTRESISTANCE >100MΩNOISE Lessthan20µVtypical DCto10kHzthrough1MΩ independentofcurrentdelivery

LEAKAGECURRENTTOGROUND Lessthan0 .5nA

OVERVOLTAGEALARM Frontpanellight

HOLD&EjECTSWITCH Manualorbyremotelogic

POWER Four9Valkalinebatteries,included

DIMENSIONS 17x5 .25x10in . (43x13x25cm)




260 DualMicroiontophoresisCurrentGeneratorOPTIONAL ACCESSORIES2933 RackMountKit,51⁄4-in .high

Capillary glass, see page 29.

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● Singleboardcomputerw/LCDtouchscreen

● Scopemodedisplayswaveforms

● 8banksof3timers,synchronous/asynchronous

● 8internaland8externaltriggerinputs

● 32separateoutputs(24BNC'sfrontpanel)

DS8000 8-Channel Digital Stimulator

● Lifetimefirmwareupgradesviaserial/Ethernet

● Unipolar,bipolar,pairedpulse,sine,ramp,custom

● Customuploadofrealbiopotentials

● 2independentfullparametermemorybanks

● GLP/GCPcompliantw/passwordprotection

TheDS8000representsaquantumleapintheperformanceoftheresearchstimulator,andisthemostadvancedstimulatoronthemarket . Withabuilt-incomputer,theentirewaveformisgenerateddigitallywithprecisiontiming .TheDS8000cangeneratestimulatingwavepatternsofacomplexityunmatchedbyanyotherinstrumentonthemarket .AbuiltindigitaloscilloscopeallowstheusertopreviewwaveformsontheLCD .AnEthernetconnectionallowstheusertotransfercustomwaveformsandupgradethesoftwareusingTCP/IPprotocolviaremoteaccess .

TheDS8000has8analogoutputs,8TTLoutputsand8combinedanalogorTTLoutputs .Eachcombinedoutputcanbecomprisedofacombinationofanyof1to8channels .Eightindependentinternaltimersandeightindependentexternaltriggersareoffered . Thebuilt-inwaveformsincludeunipolar,bipolar,andpairedpulse,aswellasstep,sine,rampandcustom .Anexternaltrigger,internalanalogchannel,internalTTLchannel,oranyoftheeightbuilt-intimerscanbeassignedtocontroleachoutputchannel .

AuniquefeatureoftheDS8000isthecapabilitytostimulatewithawaveformthatisidenticalorsimilartorealbiopotentialwavepatternsassociatedwithECG,EEGoractionpotentials .AbiopotentialwaveformcapturedbyadataacquisitionsystemmaybetransferredtoanExcelspreadsheetforeditingormodification,thenloadedintototheDS8000 .

Oneofthemainproblemsofdesigningastimulatoristhatausermightwantverydifferentstimulatingpatternsfordifferentresearchapplications .Inordertosatisfyalloftheseneeds,traditionallogicalcircuitbasedstimulatorshavecontrolpanelsthatusebuttonsandknobstogivetheuserasmuchcontroloptionsaspossible .However,evenwithafullpanelofbuttons,theselectionofthestimulatingpatternisstillverylimited .Thesetypesofstimulatorscannotgeneratecomplicatedwaveforms,suchascombinationpulsesatvaryinginterpulseintervalsandamplitudes .Althoughmicroprocessor-basedstimulatorshavemadeasignificantstepinsolvingtheseproblems,certaincomplexwaveformsarestill

impossibletogenerate .Infact,thisdecadeoldtechnologyhasseriouslimitationssinceeachcontrolbuttonhasbeenprogrammedtoperformmultiplefunctions .Moreover,itcanonlydisplaylimitedlinesofscrolledtext—nographics!Tocomplicatematters,itisalmostimpossibletoupgradethesoftwarewithnewfunctionsoncetheinstrumenthasbeenmanufactured;eventheprogrammingisawkward .

TheDS8000overcomesthehardwarelimitationsofothertypesofstimulatorsbybeingreliantonaflexiblesoftware-timinginterface .Theusercanthenapplythisdynamicallytoalmostanykindofstimulationprotocolwithoutbeingrestrictedbythehardwarelimitationsofthetraditionallogicalcircuitbasedstimulators .Inordertosuitcomplexcustomprotocols,theDS8000isdesignedtoofferauniqueflexibilitybysimplyreprogrammingthepatternoutputusingafewkeystrokesunderpull-downmenus .

AlthoughitmaybearguedthatsomefunctionsoftheDS8000canbeimplementedonastandardPC,itisimportanttorecognizethattheinherentdesignofaPCoperatingsystemmakestheaccuratedeliveryofprecisionpulseprotocolsimpossible .DespitethefactthatPCsareveryeconomical,theyaresimplynotdesignedtogeneratehighlyaccuratetimingbecausethemicroprocessorresourcesarenotprioritizedforthisfunction .Inaddition,analogwaveformgenerationisnotreadilyavailablewithoutaddingexpensiveoutputboardsandtherequiredprogrammingisnon-standard .TheDS8000platformisbasedonapowerfulsingleboardcomputerthatisfullydedicatedtothetemporalaccuracyandprecisionrequiredincurrentbiologicalandneurologicalresearch .Indeed,theDS8000DigitalStimulatoroffersallofthesesolutionsplusGoodLaboratoryPractices(GLP)complianceforresearchtraceability .

DS8000—thereisnocompetition!



DS8000 8-ChannelDigitalStimulator



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A quantum leap in the performance of the research stimulator

Paired Pulse Protocol

TheDS8000’sPairedPulsefunctionallowstheusertogeneratetriggeredpairedpulses(includingrefractoryperiod)fromasinglechannelwithouttheuseofatrainfunction . WPI’spairedpulsealgorithmsimplifiesthearduousrepetitivetasknormallyassociatedwithmanualresettingofinterpulseintervalsinrefractorystudies .Auto-incrementeliminatestheneedtooverlaptrainfunctionsfrommultiplechannelstogenerateacompleteprotocol .Thus,thereisasignificantreductioninsetuptimeandaminimizationofthepotentialforhumanerrorduringinteractiveprotocolmodification .

Fig .1showsChannel1configuredintheTRIGGEREDPAIREDPULSEmode .Inthisexample,adualpulseeventoccurs synchronouslywitheachtriggerpulsefromChannel8,whichissettotriggerevery300-ms .Theinitialinterpulseintervalissetto20ms .Subsequentinterpulseintervalsareautomaticallyincrementedby35msforeachthreeconsecutivepairedpulseevents .Theresultingpairedpulseisdisplayedinthelower traceontheDS8000scope(Fig .2) .TheuppertraceshowsthemastertriggerpulsesetuponChannel8 .

Soft Keys and GUI interface

TheDS8000employs“softkeys”,whichareprogrammablecontrolswidelyusedinseveralmenuoptionstosequentiallychangethenumericalvalueofanyvariablewaveformparameter .TheDS8000’ssoftkeysareeasilyrecognizedassingleordouble“+”and“-“signslocatedadjacenttoaparametervaluebox(Fig .-1) .Softkeysprovidequickandeasyaccesstomodifyparametervaluesontheflyduringanexperiment .TheGUIinterface(Fig .-3)enablestheusertoassigntheincrementalvalueofthesoftkeytosuittheneedsoftheexperiment .Alternatively,apop-upnumerickeypadisaccessibleforeachparametertoprogramaprecisevaluethatisnotamultipleofthesoftkey-presetincrement .

Combined Channel Assignment matrices

TheCTTL(COMBINEDTTLmatrix)andCA(COMBINEDANALOGmatrix)screenspermittheassignmentofanycombinationofthe8availableTTLorAnalogsignalstoanypermutationoftherespective(8)CTTLor(8)

Fig. 1Channel Settings

Fig. 4Combined TTL Matrix

Fig. 3Graphic User Interface

Fig. 2Scope Display

CABNCoutputs .The setupinFig .4indicatesthatallTTLchannelsareassignedtotheirrespectiveCTTLoutputswiththeexceptionof theoutputofCTTL1,whichisassignedacombinationoftheTTLsignals fromchannels4and5 .Changingassignmentsisaseasyascheckingtheassociatedbox .TheCA tabrevealsanidenticalmatrix forprogrammingtheCOMBINEDANALOGBNCoutputs .

DS8000 SpecificationsTiming parameTers

Period(totalsignalwidth) 0.04msto10,737,418.24ms

Pulsewidth 0.02msto10,737,418.24ms

BiPolargaPwidth 0.00msto10,737,418.24ms

oPeratingModes Freerun,triggered,gated,train,dC

triggers 8external,manual,ttl1-8,combined ttl1-8,timerstartorstop

trainevents 1-199

trainPulsewidth 0.02msto10,737,418.24ms(3hours)

trainPulsedelaY 0.04msto10,737,418.24ms

trainPeriod 0.06msto10,737,418.24ms

BnCoutPutConneCtors analog,combinedanalog,combineddigital(ttl)

waveForMs unipolar,bipolar,rectangular,sine,ramp,step, pairedpulse,customdefined

CustoMwaveForM 12steps/voltagepoint(1025ifremote controlled)

variaBlestePwaveForM 100points(1025ifremotecontrolled)

outPutnoise <5mvrms

tiMingaCCuraCY <100ppm

outPutvoltageresolution 5mv

Max.outPutvoltage +/-10v@+/[email protected]/step

outPutiMPedanCe 50ohmanalog,<1ohmCombinedanalog

externaltriggersYnC 40µsminimumpulsettl,CMos 20µsglitchandspikeprotection

digitali/o 5vmax10ma(input);25v@500ma (opencollectoroutput)

Mainsvoltage 85-260vaC,45-65hz50w

diMensions 13.3cmx42.5cmx25.4cm 5.25”x16.73”(19”rack)x10”

shiPPingweight 12lb(5.5kg)

aMBientteMPerature -10to+40°C;-20to+50°C(internal)

huMiditY Max.95%relativehumidity,non-condensing

SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.



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Digital Linear Stimulus Isolator

TheintroductionofWPI’sdigitalstimulator(DS8000)causedamajorstirinthephysiologycommunitysincetherewasnodigitalisolatoravailablethatcouldtakefulladvantageoftheDS8000’sadvancedfeaturesandspecifications .Tosolvethisproblem,WPIdesignedarevolutionarynewbreedofdigitallinearisolatorswithhighcomplianceandhighisolation—theDLS100beingthefirstoffspringofovertwoyearsofresearchdesign .ThenewDLS100isapreferentiallyoptimizedforapplicationsinwhichtheDS8000digitalstimulatorisused .

DLS100connectstotheDS8000viaaflexiblecablethroughwhichitreceivespowerandstimulussignalsinadigitalformat .UptoeightDLS100isolatorscanbeconnectedindependentlytooneDS8000 .VeryhighisolationisachievedthroughtheuseofopticalcouplingofthedigitalsignalandagalvanicallyisolatedDCpowersupplywithintheDLS100 .Unlikesomeothermulti-channelisolators,thisdigitalisolatorcanbelocatedatthesiteoftheexperiment,allowingtheuseofshortconnectingleadsandtherebypreservinghighisolationandfastsignalriseandfalltimes .

TheDLS100operatesintwomodes:curentsourceorvoltagesource .Inthecurrentsourcemode,theoutputcurrentisproportionaltotheamplitudeandpolarityofthesignalgeneratedbytheDS8000 .Inthevoltagesourcemode,theoutputvoltageisproportionaltotheamplitudeandpolarityofthesignalgeneratedbytheDS8000 .TheDLS100hasuser-selectablepush-buttonswitchesthatselectdifferentcurrentorvoltageranges(thefull-scalecurrentfrom100nAto10mA,orthe10Vand100Vfull-scale,respectively) .ThreestatusindicatorsontheDLS100indicatepoweron,activity(presenceofsignalfromDS8000)aswellasanalarm(over-rangecondition)indicator .Over-rangecanoccurwhentheresistanceoftheload(theexperiment)istoohighforthecurrentorvoltagethatisdemandedfromtheDLS100 .

DLS100 SPECIFICATIONSCURENTSOURCEMODE Full-scale*Current 10mA,1mA,100µA,10µA,1µA,bipolar ComplianceVoltage ±100volts OutputImpedance Greaterthan100Megohms Zero-signalLeakage Lessthan0 .01%offull-scalerangesetting ≤10mV@100V/10,000Ohms, 10mAScale=<1µALeakage Linearity Betterthan0 .05%offull-scalerangesetting Bandwidth Rangeandloaddependant:20kHzwith 10Kloadand100µAoraboverange .**

VOLTAGESOURCEMODE Full-scale*Voltage ±100volts MaximumCurrent 10mA OutputImpedance Lessthan1ohm Zero-signalOffset Lessthan1mv Linearity Betterthan0 .05%offull-scalerangesetting Bandwidth 50kHz

ISOLATION Resistance Greaterthan1000Megohms Capacitance Lessthan10pF,fromoutputterminalsto DS8000andearthground

POWERREqUIREMENTS +12voltsand+5volts,suppliedbyDS8000

DIMENSIONS 14x9x3 .5cm(5 .5x3 .5x1 .5in .)

OUTPUTTERMINALS Mini-bananajacks

CONNECTINGCABLE 150cm(5ft)

Top View

for use with DS8000 Digital Stimulator



DLS100 DigitalLinearStimulusIsolator501670 Adapter,DualMini-Banana-to-BNC(F) 83016 ReplacementCable,DLS100-to-DS8000



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A310 ACCUPULSER™ SPECIFICATIONSTIMING PARAMETERS

EVENTINTERVAL 100µsto1000s*

EVENTDELAY 10µsto100s*

PULSEWIDTH 10µsto100s*

TRAINDURATION(ENVELOPE) 100µsto1000s*

PULSEINTERVAL 20µsto100s*

OUTPUTS

SYNC 5µs,TTL,and5VCMOScompatible, 20 mAmax .

MONITOR 10-15V,50mAmax .

ISOLATOR TTL&5VCMOScompatible,20mAmax .

VARIABLE(PosorNeg) PULSED/DC LOWRANGE HIGHRANGE Range 0to±1V 0to±10V Resolution 1mV 10mV

NOISE Pulsedat100kHzbandwidth <500µV DCWideBand <500µV

OUTPUTIMPEDANCE <1ΩINPUTS

EXTERNALSYNC Accepts1-µsminimumpulses TTL,CMOScompatible

EXTERNALGATE Accepts1-µspulsetocontinuous TTL,CMOScompatible

POWER 95-130Vor190-260V,switchselectable singlephase,50/60Hz

DIMENSIONS 17x5 .25x10in .(43x13x25cm)

SHIPPING WEIGHT 14lb(6 .4kg)

*Continuously variable in six ranges. All accuracies better than 1% of set value. 50-kHz maximum pulse frequency.

Combining the accuracy of digital electronics with the convenience of analog controlsApulsegenerator/stimulatorcombiningthereproducibilityandaccuracyofdigitalelectronicswiththefineresolutionandcontinuousadjustmentpossiblewithanalogcircuitry .Alltimingparametersareenteredwithten-turnreadablepotentiometersandsix-positionrangeswitches .Outputsareaccuratetowithin1%ofthesetvalue .

Pulses canbecreatedincontinuousrun,single-shot,ortrain/burstmodes .Durationofthetrain/burstiseasilycontrolledusingtheonboardenvelopegeneratororbyusingeitheroftwoexternalgatinginputs .UsedinconjunctionwiththeA360,A365,A385,orA395,bipolarpulsesortrainsmaybeeasilyproduced .OutputstimuluscanbefedthroughIntra767orDuo773foriontophoresis .Footswitchallowshands-freeoperation .

Three separate outputs areavailableonthefrontpanel .AMonitoroutputprovides10-15Vsignals(upto50mA)forviewingtheoutputonanoscilloscopeorforcontrollingotherdevices .Thestimulator’ssignal,simultaneouslyavailableattheIsolatoroutput,issufficienttodriveanyWPIA300Seriesstimulusisolator(A360,A365,orA385)andisalsoTTLandCMOScompatible .TheVariableoutputcanprovidesignalsvaryingbetween±10Vwitharesolutionof1mV .Separatevariableoutputsareprovidedforpositiveandnegativesignals .

Stimulus Isolator (A360)

A310

Optional footswitch #3259



A310 Accupulser™SignalGeneratorSpecify line voltage

OPTIONAL ACCESSORIES3259 FootswitchforA3102933 RackMountKit,51⁄4-in .high

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A300 Pulsemaster™

Multi-Channel Stimulator

ThePulsemaster™(ModelA300)isWPI’sthirdgeneration,multichannel,pulse/traingenerator/stimulatorthatcombinesthesuperbaccuracyofdigitalelectronicswiththe“you-see-what-you-get”displaysonlyavailableonsingle-channelproducts .Inonecompactrackmountableenclosure,thePulsemastercontainsaneventintervalgenerator,fivepulsetrainchannels,twomixingchannelsandaveryquietvariablevoltageoutputchannel .Systemtimingisaccurateto100ppm;outputtimingiscontinuouslyvariablein0 .1%offullscaleincrementsoverarangeofeightordersofmagnitude .Bright,three-digitLEDdisplayscontinuouslyandsimultaneouslyshowallthevariabletimingparameters .

ThePulsemasterisdesignedforeaseofuseandflexibility .Eachchannelcanbeoperatedsynchronizedwiththeonboardeventintervalgenerator,triggeredmanuallyfromanyotherchannelorexternalsource,andasanindependentasynchronouspulsegenerator .Exceptfortheexternalsource,allchannelinterconnec-tionsareaccomplishedonthepanel,withouttheuseofcables .TheoutputfromeachchanneliscompatiblewithstandarddigitalcircuitryandisalsodesignedtodriveWPI’sA300seriesstimulusisolators .Ifdesired,anychannel’soutputmaybeinternallyconnectedtothevariablechannel,whoseamplitudecanbecontinuouslyadjustedfrommillivoltstotenvolts .

The Event IntervalTheEVENTINTERVAListheheartbeatofthePulsemaster .Basedon

ahighlyaccurateandstablecrystaloscillator,theEVENTINTERVALgeneratessynchronizationpulsesatregularintervals .Thewidthofthesyncpulsesisfixedatapproximately6 µs,buttheirrepetitionintervalispaneladjustablefrom10µsto999s,usingthedisplayanditsassociatedswitches .SyncpulsesmayalsobegeneratedatrandomorirregularintervalsbyusingtheSINGLEEVENTortheEXTernalSYNCmode .ThesyncpulsesareinternallydistributedtothefivePULSETRAINchannelsandarealsoavailableexternallythroughtheSYNCOUTconnector .

The Pulse TrainTherearefivePULSETRAINchannelsinthePulsemaster .Exceptforsharingacommonpowersupply,eachisanindependentinstrument .Eachchannelhastwoadjustabletimingparameters,DELAYandWIDTH,whichincombinationwithinternalorexternalsignalsmaybeusedtocreateavarietyofpulseandtrainwaveforms .DELAYandWIDTHparameterscanbechangedwhiletheinstrumentisoperating .

ThebasicdifferencebetweenPULSEandTRAINmodesisthenumberofpulsesthatcanbegeneratedpersyncpulse .InPULSEmode,onlyonepulseisgeneratedforeachsyncpulse .Asthesyncpulseisreceivedfromoneoftheselectedinputsources,theleadingrisingedgeofthepulseisrecognizedandaDELAYtime/pulseWIDTHpairisgenerated .InTRAINmode,multiplepulsescanbegeneratedpersyncpulse .AsinPULSEmode,astheleadingrising

An integrated five-channel pulse generator/stimulator includingone interval generator, five pulse or train channels, two mixer channels and one

very quiet variable voltage output stage





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A300 PULSEMASTER SPECIFICATIONS

EVENT INTERVAL CHANNEL

OperatingModes EXTernalSYNC,SINGLEEVENT,CONTINUOUSON

Input EXTSYNCaccepts≥1-µspulses;TTL,CMOS,

RS232Ccompatible

Timing EVENTINTERVAL10µsto999s(100kHz-0 .001Hz),

±0 .1%offullscale,continuouslyvariablein0 .1%offull

scaleincrements,throughthreeordersofmagnitude,in

sixranges

Output SYNCOUTpulseof≈6µs,TTL,5VCMOScompatible

PULSE TRAIN CHANNEL(5provided)

OperatingModes EXTernalSYNC,SELFSYNC,manualSINGLEevent,

syncfromEventInterval,syncfromanyofotherfour

PulseTrains,syncfromoneoftheMIXers,off,

TRAIN/PULSE

Input EXTSYNCaccepts≥1-µspulses;TTL,CMOS,

RS232Ccompatible

Timing DELAYandWIDTH10µsto999s,±0 .1%offullscale,

continuouslyvariablein0 .1%offullscaleincrements,

throughthreeordersofmagnitude,insixranges

( .0005Hzto50kHzintheSELFSYNCmode)

Output OUTPUTPULSE/TRAINofpresettiming,TTL,5V

CMOScompatible,4mAsinkandsource

MIXER CHANNEL(2provided)

Inputs AnycombinationofanEXTernalpulse,theoutputsof

thefivePulseTrainchannels,andDCcontinuousON/

DCMOMentaryEXTINPUTaccepts≥1-µspulses;TTL,

CMOS,RS232Ccompatible

Output OUTPUT,TTL,5VCMOScompatible,4mAsinkand

source

VARIABLE CHANNEL

Inputs OutputfromanyonePULSETRAINchannel

oroneofthetwoMIXERchannelsorDC

Output 0to+1Vlowrange,1mVresolution

0to+10Vhighrange,10mVresolution

5mAmaxsinkandsource

OutputImpedance <1ohm

Noise <500µVpeak@100kHzbandwidth,PULSEDmode

<500µV,wideband,DCmode

SignalGround Floating,i.e.,notconnectedtochassis

POWER 95-135Vor220-240V,50/60Hz

BATTERIES Three1 .2VDC,sizeAA,NiMHbatteries

DIMENSIONS 8 .5x19x8 .75in .(22x45x22cm)


edgeofthesyncpulseisrecognized,apulseWIDTH/DELAYpairisgenerated .Pulses(WIDTH/DELAYpairs)arecontinuouslyproducedaslongasthesyncpulseisstill“high”attheconclusionoftheDELAYtime .

BymeansoftheINPUTSELECTswitch,syncpulsescanbereceivedfromanexternalsourcethroughtheEXTSYNCconnector,manuallybytheSINGLEswitch,fromanyoftheotherPULSETRAINchannels,oroneoftheMIXERchannels .ThechannelmayalsobedisabledbyswitchingtooneoftheOFFpositions .

EXT SYnC position:permitscontrolofthePULSETRAINchannelbyotherinstrumentsandcomputers .

SELf SYnC position:thechannelbecomesafreerunningpulsegen-erator .ThesymmetryofthewaveformcanbeadjustedbyvaryingtheDELAYand/ortheWIDTHtimes .

SInGLE EVEnT position:pulsesaregeneratedatyourdiscretion .EverytimetheSINGLEbuttonispressed,oneandonlyoneDELAY/WIDTHsequenceisgenerated .

EI position:connectstheoutputoftheEVENTINTERVALgeneratortotheinputofthePULSETRAINchannel .ForeveryoutputsyncpulsefromtheEVENTINTERVALgenerator,oneDELAY/WIDTHpairisgenerated .

PT positions(4):internallyconnecttheoutputsfromtherespectivePULSETRAINchannelstotheinputofthisPULSETRAINchannel .InthePULSEmode,foreveryOUTPUTpulsefromtheotherselectedPULSETRAINchannel,oneDELAY/WIDTHpairisgeneratedfromthischannel .IntheTRAINmode,pulsesaregeneratedfromthischannelaslongasthepulsefromtheotherchannelremains“high .”

MIX positions(2):internallyconnecttheoutputsfromtherespectiveMIXERchannelstotheinputofthisPULSETRAINchannel .InthePULSEmode,foreveryOUTPUTpulsefromtheMIXERchannel,oneDELAY/WIDTHpairisgeneratedfromthischannel .InTRAINmode,pulsesaregeneratedfromthischannelaslongasthepulsefromtheMIXERchannelremains“high .”

OuTPuT connector:suppliesthewaveformsgeneratedbythePULSETRAINchannel .ThisOUTPUTisdesignedtodriveWPI’sA300seriesstimulusisolators .Itisalsousefulforsynchronizingotherinstruments(recorders,oscilloscopes,computers,etc .)withthepulsesgeneratedfromthePulsemaster .

The MixerTheMIXERdoeswhatitsnameimplies,itcombinesanyoralloftheoutputsofthePULSETRAINchannelswithexternalsignalsintoonewaveform .Itcanalsoprovideacontinuous(DCON)ormomentary(DCMOM)“high”levelsignal .TheMixerOUTPUTconnectorsup-pliesthecombinationwaveformsgeneratedbytheMIXERchanneltodriveWPI’sA300seriesstimulusisolatorsortosynchronizetheoperationofotherinstrumentswiththePulsemaster .

The Variable ChannelTheVARIABLEchannelcanreplicatetheOUTPUTwaveformsfromanyofthePULSETRAINorMIXERchannelsatamplitudesthatcanbevariedfrommillivoltstotenvolts .Thechannelalsoprovidesaverylownoise,adjustableDCvoltagesource:theDCmodewhichconvertstheVARIABLEchannelintoaconstantvoltagesource .TheOUTPUTconnectorsuppliestheamplitudemodifiedwaveformortheDCvoltageleveloftheVARIABLEchannel .



A300 Pulsemaster™Multi-ChannelStimulatorSpecify line voltage

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Timing

Pulseintervalandwidtharesetwithsingle-turncontinuouslyvariablecontrolsfrom5msto5 .5sinthreeranges .Pulsewidthiscontinuouslyvariablefrom50µsto550msinfourranges .

Modes of operation

InFREERUN,Isostim™generatescontinuoussquarewaves .InEXTGATEorEXTSYNCmodes,externallyappliedpulsescangeneratetrainsorsingleevents .Singlepulsesoffinitedurationcanbeproducedusingapush-buttonontheinstrument’sfrontpanel .EXT/DCmodeconvertsIsostimtoapassivestimulusisolator .

Dual tone audible alarm

Atonesoundswhenanopencircuitisdetectedorwhensystemcomplianceisreached .Asecondtone,whichsoundswhenasignalisappliedtotheinput,canonlybeheardifthebatterieshavesufficientchargetooperatetheisolator .Aviolationlightadviseswhenpulsewidthexceedstheinterval .

ISOSTIM™ SPECIFICATIONSTIMING PARAMETERS

Interval 5msto5 .5scontinuouslyvariable inthreeranges(0 .18to200Hz)

Pulsewidth 50µsto550mscontinuously variableinfourranges

INPUT

Externalsync Accepts1-µsminimumpulses

Externalgate Accepts1-µspulsetocontinuous

Ext .commandvoltagethreshold 2 .5Vat3 .5mAmin .,8 .5Vmax .

OUTPUT

Waveform DC,pulsefrominternaltimingor externallygeneratedpulse

Currentranges 0-1mA,0-10mA

Loadvoltageexcursion(compliance) 100Vnom .,150Vmax .

Outputpolarity Reversible,manualswitch

Currentrisetimeanddelay 8µs,typical(1KΩload)

Currentfalltimeanddelay 10µs,typical(1KΩload)

Leakageresistance,output toground1012Ohms

Optocoupler 2500Vratedmin .breakdownvoltage

POWER

DryCell(VersionD) 16alkaline9Vbatteriesincluded

Rechargeable(VersionR) 16rechargeableNiMH9Vbatteriesincl

DIMENSIONS 8 .5x3 .5x4 .9in(22x9x12cm)


Isostim™ Stimulator/IsolatorCombining the ease of use and accuracy of WPI’s 300 Series stimulators with the power output of a stimulus isolator

A362 Battery ChargerRequired for A320R, A365R and A395RRechargesthehigh-voltagenickel-cadmiumorNiMHbatterystackintheA320R,A365RorA395R .LEDlampindicateschargingstatus .Fullchargeovernight .Dimensions:2 .8x4 .1x5in .(7x10x13cm) .Shippingweight:4 lb(1 .8kg) .

Current delivery

Stimuluscurrentsupto10 mAcanbesetonthefrontpanelwithacontrolknobandatwo-positionrangeswitch .Outputcurrentisload-independent .

Power

IsostimmodelA320Dispoweredbyreadilyobtainable9-voltalkalinebatteries(included) .Underaverageusethesewilllastseveralmonthsbeforereplacementisrequired .TherechargeableA320Rissuppliedwithanickelmetalhydridebatterystackwhichprovides10-12hoursofoperationbeforerechargeisrequired .The A362 Battery Charger must be used with the A320R.



A362 BatteryChargerforA320R,A365R,A395RA320RC A320RwithCharger(A362)A320D Isostim™Stimulator/IsolatorA320R Isostim™Stimulator/Isolator(rechargeable)


OPTIONAL ACCESSORIESDRL DummyLoadResistorKit(setof3)13347 BNC-to-DoubleBananaAdapter



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Stimulus Isolators / Precision Current Sources

DRL — Dummy Load Resistor KitConvertscurrentoutputtoprecisevoltages .

Model A365includesthesamefeaturesandspecificationsasA360butwiththeaddedcapabilityforautomatedbipolarpulsingforzeronetchargeonbiologicalpreparations .

Polarity —Outputpolarityisdeterminedbyapushswitchonthefrontpanel .Bipolarcurrentistoggledbythecommandwaveform,settingalter-natingpulsesaspositiveornegative .

A365

A365 SPECIFICATIONSOUTPUTWAVEFORM DCorcurrentpulse

OUTPUTCURRENTRANGES 0 .1,1 .0,and10mA

CURRENTAMPLITUDEERROR 0 .5%offullscale,max .

CURRENTRESOLUTION 0 .1%offullscale,typical

OUTPUTLOADVOLTAGE EXCURSION(COMPLIANCE) 100V

EXTERNALCOMMANDTHRESHOLD 2 .2Vat2 .6mA,min .8 .5V,max .

OUTPUTPOLARITY Reversible,manualswitchorautomatic

CURRENTRISETIME&DELAY 6µs,typical(1KΩload)

CURRENTFALLTIME&DELAY 10µs,typical(1KΩload)

OUTPUTTOGROUNDRESISTANCE 1012ΩOPTOCOUPLER 2500V,ratedmin .breakdownvoltage

POWER ModelA365D(drycell) 16alkaline9Vbatteries,included ModelA365R(rechargeable) 16rechargeableNiMH9Vbatteriesincl .

DIMENSIONS 8 .5x3 .5x5in(22x9x12cm)


A360LA SPECIFICATIONSOUTPUTWAVEFORM DCorcurrentpulse

OUTPUTCURRENTRANGES 0 .1,1 .0,and10mA

CURRENTAMPLITUDEERROR 0 .5%offullscale,max .

CURRENTRESOLUTION 0 .1%offullscale,typical

OUTPUTLOADVOLTAGE EXCURSION(COMPLIANCE) 100Vminimum,130Vnominal

EXTERNALCOMMANDTHRESHOLD 2 .2Vat2 .6mA,min .8 .5V,max .(TTL)

OUTPUTPOLARITY Reversible,manualswitch

CURRENTRISETIME&DELAY 2µs,typical(1KΩload)5µs@10KΩCURRENTFALLTIME&DELAY 5µs,typical(1KΩload)8µs@10KΩOUTPUTTOGROUNDRESISTANCE 1012ΩOPTOCOUPLER 2500V,ratedmin .breakdownvoltage

NOISE <0 .1µA(10mA), <50nA(1mA,100µA)

CURRENTLEAKAGE ±10nA±0 .01%offullscale

POWER Two6-Vlead/acidbatteries,2 .4Ah

RECHARGETIME 4hours

RECHARGERINCLUDED 100-240VAC,50-60Hz,universal

DIMENSIONS 4 .5x3 .5x5 .5in .(11x9x14cm)


Activatedbyconventionallogic-levelcommands,Model A360LAcanbegatedbyanypulsegenerator,stimulator,orcomputeroutput .

Dual tone audible alarms —Atonesoundswhenanopenelectrodecircuitisdetectedorwhensystemcomplianceisreached .Asecondoptionaltonesoundswhenasignalisappliedtotheinput .Atestswitchisalsoprovidedtocheckbatterycharge .

Current delivery —Stimuluscurrentsaresetusingathree-digitcontrolknobandathree-positionrangeswitch .Outputcurrenttrackscontrolsettingstobetterthan1% .Outputcurrentisloadindependent;voltagesufficienttopushthedesiredcurrentthroughtheloadisautomaticallydeveloped,subjectonlytocompliancelimits .ModelA360LAproducesupto10milliamperecurrent,inthreeranges,atmorethan100voltscompliance .

Polarity —Outputpolarityisdeterminedbyathree-positionswitchonthefrontpanel(+/-/off) .

A360LA



A360LA HighVoltageIsolator,chargerincludedSpecify line voltage

OPTIONAL ACCESSORIESDRL DummyLoadResistorKit(setof3)13347 BNC-to-DoubleBananaAdapter

A365D HighVoltageIsolator,Bipolar,alkalinebatteriesA365RC A365Rwithcharger(A362)A365R HighVoltageIsolator,Bipolar,rechargeableA362 BatteryChargerforA320R,A365R,A395R


OPTIONAL ACCESSORIESDRL DummyLoadResistorKit(setof3)3468 DualRackMountKitforA3653469 SingleRackMountKitforA36513347 BNC-to-DoubleBananaAdapter

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A382 SPECIFICATIONSPOWER 95-135Vor220-240V,50/60Hz

DIMENSIONS 8 .5x3 .5x5in(22x9x12cm)


A385 SPECIFICATIONSOUTPUTWAVEFORM DCorcurrentpulse

OUTPUTCURRENTRANGES 1,10,and100mA

CURRENTAMPLITUDEERROR 0 .5%offullscale,max

CURRENTRESOLUTION REPEATABILITY 0 .1%offullscale,typical

OUTPUTLOADVOLTAGE EXCURSION(COMPLIANCE) 36V

EXTERNALCOMMANDVOLTAGE

THRESHOLD 2 .2V,min

OUTPUTPOLARITY Reversible,manualswitch,or electronicallyswitchedbipolardelivery

CURRENTRISETIMEANDDELAY 6µs,typical(1KΩload)

CURRENTFALLTIMEANDDELAY 10µs,typical(1KΩload)

OUTPUTTOGROUNDRESISTANCE 1012Ω

OPTOCOUPLER 2500V,ratedminimumbreakdown voltage

POWER Sixrechargeablelead-acidbatteries (RequirescompanionchargerA382)

DIMENSIONS 8 .5x3 .5x5in .(22x9x12cm)


A385 High Current Stimulus IsolatorDeliverspositive,negative,orbipolarcurrents .Forbipolardelivery,polarityoftheoutputistoggledtotheoppositestatewitheachpulsepresentedtotheinput .Pulsedurationiscontrolledbyanexternallyappliedvoltage .InputconnectorisastandardBNC,allowingsignalsfromanysource—suchascomputerD/AorI/Olines—tobeused .

Outputamplitudeissetona3-digit,ten-turndialasapercentageoftherangeselected:forexample,asettingof45 .6inthe0-10mArangetranslatesto4 .56mAattheoutput .Accuracyandrepeatabilityareexcel-lent .Designedforsubcutaneousstimulation,maximumoutputvoltageatthestimulatingelectrodesis36volts,reducingthepossibilityofseriousaccidentaltranscutaneousshocks .Acompliance/outputalarmsounds

whenthe36-voltlimitisreached .Internalcircuitrymaintainselectrodesshort-circuitedduringinactiveperiods(“electrodeexhauster”feature) .A385isnotappropriatefortranscutaneousstimulation .

The1 .2amp-hourratingofthesixheavy-dutylead-acidrechargeablebatteriesensuresthatexperimentswillnotbeinterruptedbydeadbatter-ies—evenatpeakcurrents .Indicatorlightsandaudiblealarmskeeptheuserconstantlyapprisedofbatterychargestatus .ThesebatteriesmustberechargedbytheA382SystemChargerdesignedespeciallyfortheA385 .

Combines optical isolation with a ±100 mA current generator

A382 Battery ChargerAninnovativethree-stepcharger,A382employsfast,medium,andtricklechargesatasafe,lowcurrent,greatlyextendingbatterylife .Afterafastinitialphase,thechargerautomaticallyswitchestoaconstantvoltagemode .Whenchargingiscomplete,thechargerswitchestothetrickle-chargemode .LEDlampsindicatechargingstatus .(For use only in charging batteries installed in the A385.)



A385RC A385RwithA382Charger A385R HighCurrentIsolator,rechargeableA382 BatteryChargerforA385(seebelow)


OPTIONAL ACCESSORIES3468 DualRackMountKit



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Current Delivery —A10Vinputproducesthemaximumoutputcurrentforthecurrentrangeselected, i.e.,100µA,1 mA,or10 mA .FrontpanelcontrolsallowDCcurrenttobegenerated .Externallyappliedsignalscanbesuperimposedsimultaneously(DCoffset) .Warninglampsindicateopencircuitorexcessivecurrentconditions .

Digital Meter —MeasuresDCoraverageoutputcurrent .

Overload Lamps —Indicatewhenoutputvoltagehasreachedpositiveornegativecompliancevoltagelimit .

A362 Battery ChargerRequired for A320R, A365R and A395RRechargesthehigh-voltagenickel-cadmium or NiMH battery stack intheA320R,A365RorA395R .LEDlampindicateschargingstatus .Fullchargeovernight .Dimensions:2 .8x4 .1x5 in .(7x10 .5x12 .7cm) .Shippingweight:4lb(1 .8kg) .

A395 SPECIFICATIONSOUTPUTCURRENT,Imax 3ranges:100µA,1mA,and10mA

OUTPUTVOLTAGERANGE ±70V

OUTPUTBANDWIDTH 10kHz(measuredacross1KΩloadR)

INPUTRESISTANCE >20MΩINPUTVOLTAGE@Imax ±10volts

INPUT/OUTPUTLINEARITYERROR <0 .5%

RISE,FALLTIME 26µs@10KΩ

POWER ModelA395D 17alkaline9Vbatteries ModelA395R 17rechargeableNiMH9Vbatteries

DIMENSIONS 6 .5x4x3 .5in .(16x10x9cm)


AllWPIstimulusisolatorsaredesignedtosupplyconstantcurrentbecausecurrentthreshold(notvoltage)isthemostquantitativelyreproducibleparameterforstimulationofnerveandmuscle .ModelA395dispensescurrentreproduciblyfromitsOutputterminals;theamplitudebeingdeterminedbytheselectedcurrentRANGEandtheinputvoltage .Currentamplitudeis“constant”,thatis,loadresistanceindependent,providedthattheIxR(load)productdoesnotexceedtheavailablebatterysupplyvoltage .Anaudiblealarm(thecompliancealarm)willsoundifIxRreachesthislimit .ModelA395Dcangenerateavoltageof70voltsormoreacrossitsOUTPUTterminals .Thus,theusercanbesurethattheamplitudeofthecurrentwillbeasdialedaslongasthevoltagedropaccrosstheload(stimuluselectrodepath)doesnotreachthemagnitudeofthesupplyvoltage .Thecompliancealarmwouldthenbeheard .Theuserwouldthenknowthat(a)toomuchcurrentwasdialedforagivenloador(b)inter-electroderesistancewastoohighortheelectrodecircuitpathwasopen(thisisillustratedbythequickInstrumentTestbelow) .

ModelA395generatesanoutputcurrentofarbitrary(user-defined)waveshape;DC,AC,pulse,andcombinationsthereof .Batteryoperated,andphotoelectrically-isolatedfromtheinputvoltagedrive,theinstrumentregeneratesoutputcurrentswhicharelinearlyproportionaltotheanalogvoltagewaveformsprovidedbyyourD/Aconverterorsignalgenerator(seediagrambelow) .

TheA395isideallysuitedfordataacquisitionandstimulatorgenerators .Itcanbeeasilydaisy-chainedformutiplechannelrequirements .

10 V

0 V

-10 V

+100 µA, +1 mA, +10 mA

0

-100 µA, -1 mA, -10 mA

Accepts

analog

input

A395 Linear Stimulus IsolatorReplicates a programmed waveform of any shape or polarity

INPUT

OUTPUT



A395RC A395RwithCharger(A362) A395D LinearStimulusIsolatorA395R LinearStimulusIsolator,RechargeableA362 BatteryCharger


OPTIONAL ACCESSORIES3468 DualRackMountKit3469 SingleRackMountKit

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Precision Stereotaxic InstrumentsSingle and Dual Manipulator Models

WPI’sPrecisionStereotaxicInstrumentisbuiltaroundthetime-provenU-framedesignconcept,providingstability,andadaptabilitytomostspecies .Precisionalignmentensuresaccurateplacementofelectrodes,micropipettes,andotherdevices .Itisidealforresearchersinneedofaversatile,reliableinstrumentforstereotaxicprocedureswithsmallanimals .

Versatility of PositioningThemanipulatorarmcontrolsmedio-lateralandverticalpositioningvialeadscrewswith80mmoftravel .Thisallowsthefastestpositioningpossible,consistentwithliningupthescaleseasilyatagivencoordinate .Theantero-posteriormovementiscontrolledviaadovetailslidemovement,with80mmoftravelpossibleineachdirection .Auniversaljointallowstheinvestigatortochangetheangleoftheprobeupto90°ineithertheantero-posteriorormedio-lateralplanes .Thelockingmechanism

● 5 mm linear movement per revolution on each axis

● Absolute lock at 90º (vertical)

● Entire frame is electri-cally continuous, ideal for Electrophysiology

● Accessories available for use with a wide variety of small animals

willholdanyanglepositionwithoutdriftorcreep .Italsoprovidesanabsolutelockat90°vertical .

Easily Read ScalesAllscalesareorientedtobereadeasilyfromtheopenendofthe“U” .Thisisthepositionfromwhichmostscientistsprefertowork .Thenumeralsonthescalesareclearandeasytoread .Precisealignmentwithfacingvernierscalesgivesaccurateresolutionto0 .1mm .VernierscalescanalsobeaugmentedwithadigitalLCDpositionreadoutsimilartoadigitalVerniercaliper .Thisoptionextendsthereadableresolutionto10microns .

Convenient for Electrophysiology TheentireStereotaxicframeincludingthedovetails,manipulatorarmsandbaseareelectricallycontinuous .Groundingoftheentireframeincludingthebaseplatecanbe

● Large, easy to read vernier scales– accurate to 100 microns

● Triple lead screws for fast positioning

● 80 mm of vertical, lateral and anterior-posterior travel

Dual Manipulator Stereotaxic Frame 502603.

Digital readout aids accurate positioning — 502900.





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accomplishedbyconnectingtheprovidedgroundingstudtoearth .Thisisidealforelectrophysiologicalstudieswheretheanimalandsurroundingstructuresneedtobegroundedtoreduceelectricalnoise .

Dual Manipulator ModelThe502603DualManipulatorhas3-dimen-sionalmanipulatorarmsonbothsides .Thesecondmanipulatorarmcanbeinstalledattimeoforderoraddedlaterbytheuser .Dualmanipulatorsareveryusefulinstudiesinwhichdoubleinjectionsorsimultaneousstimulationandrecordingarenecessary .

Included ComponentsBoththe502600and502603includetheU-framebaseplate,manipulatorarm(s),standardprobeholder(s)withcornerclamp,andRatAdaptor(noseclampassemblyand18°taperearbars) .The502650and502653include

non-puncture45°earbarsinsteadof18°earbars .

Selection of AccessoriesSpeciesadaptorsareavailabletofitrat,cat/monkey,mouse,guineapigandsmallbird .Probeholdersandspeciesadaptorsfor“U”framestereotaxicinstrumentsfromothermanufacturersaregenerallycompatiblewiththeWPI’sstereotaxicframe .

SAVEwhen you buy an

UltraMicroPump with any of these stereotaxic frames!

See page 232.

TheWPIMouseandNeonatalRatAdaptoremployslight,Delrin®earbarswithtaperedpointsononeendandrubberpadsontheothertofacilitatesurgeryonmiceandratpups .

Earbarsmaybeindependentlyadjustedinheighttoleveltheskull .Laserengravedscalesshowtheverticalpositionsoftheearbars .Atoothbarandnoseclampsecuresthenose .Awellinthethickaluminumbodymaybefilledwithdryiceandalcoholforhypothermicanesthesiaofneonatalanimals .The502063Adaptorclampssecurelyontherightsideofthe“U”frameofthestereotaxicinstrument .

Modelorganismswithsoftskulls,suchasthemouseandtheneonatalrat,aredifficulttosecurefirmlyinatraditionalstereotaxicinstrument .Thestainlesssteelearbarsusedforadultratsaretooheavytoprovideasecureholdwithoutcompressingthesidesoftheskullinward(whichclosestheairwaysandimpedesbloodflow .)Further,theneonatalratpuphasnoexternalopeningoftheauditorycanalintowhichtoinserttheear

barpoints .

Thespecializedjawholdercuffs(502080)securelyclampthezygomaticprocessesoftheskull .Thejawholdercuffsfitovertheendsoftheearbars,replacingtherubberpads,providinganalternativenon-invasivemeansofsecuringtheanimal’sheadinthestereotaxicstage .Theseoptionsfor“triplepoint”securingoftheanimal’sheadinthisadaptormakeitthemostversatilemousestereotaxicavailable .Setoftwojawholdercuffsincluded .

Mouse and Neonatal Rat AdaptorFor 502600 Series Stereotaxic Instruments

502063

502062

502204



502600 WPIStereotaxicFramewith18°EarBars 502650 WPIStereotaxicFramewith45°EarBars 502603 DualManipulatorStereotaxicFramewith18°EarBars 502653 DualManipulatorStereotaxicFramewith45°EarBars 502045 U-Frame,BasePlateandRatAdaptoronly,withoutManipulatorArm502204 RatAdaptor502062 MouseAdaptor TAXIC-600 WPIStereotaxicFramewith18°EarBarsplusUMP3System TAXIC-650 WPIStereotaxicFramewith45°EarBarsplusUMP3System TAXIC-603 DualManipulatorStereotaxicFrameplusUMP3System TAXIC-653 DualManipulatorStereotaxicFramewith45°barsplusUMP3System502201 V-clampforUMP3

502063 Mouse/NeonatalRatAdaptorfor502600502080 jawHolderCuffs,setof2

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Concentric Bipolar ElectrodesExcellent for shielded macro recording as well as evoked potentials — especially well suited for bipolar stimulation

Thetungstenelectrodeissharpenedtoapointandis75micronsindiameter .TheouterstainlesssteelconductorisinsulatedwithPolyimidetubingtowithin0 .2mmoftheendofthestainlesssteeltube .AlsoavailablewithouttheouterPolyimideinsulation .

Gold-plated connector pin Parylene coating 2-, 3-, 5-inch lengths Gold-plated connector pins Polyimide tubing0.014" O.D. for 0.005" O.D. WE GlueShrink tubing

3" and 5" total length

Parylene-CInsulation

Tip separation125 microns

5 mmtypical

Exposedtips

Gold-plated connector pinPolyimide tubing

0.016" O.D. Glue seal

3" total length

3µ Parylene

5 mmtypical

29 gauge stainless steel tubing 0.003" IR

Gold-plated connector pin

29 gauge stainless steel tubing

Polyimide tubingGlue

5 mm

0.003" Iridiumor 0.005" Tungsten electrode,Parylene-coated

12"

* Parylene is a trade mark of Union Carbide. Kapton is a trade mark of DuPont. Elgiloy is a trade mark of Elgiloy Ltd.

Metal MicroelectrodesSuperior microelectrodes for outstanding extracellular recording — tungsten, iridium, platinum-iridium, and Elgiloy®

EXPOSED TIP DIMENSIONS(nominal)

Nominal Platinum Pure Impedance Tungsten Elgiloy Iridium Iridium 0 .1MegΩ 100µ 120µ 60µ 45µ 0 .5MegΩ 55µ 66µ 18µ 14µ 1 .0MegΩ 30µ 36µ 10µ 10µ 2 .0MegΩ 12µ 15µ 6µ 5µ 5 .0MegΩ 5µ 6µ 3µ 2 .5µ

Kapton* tubing,indicatedby“KT”inthepartnumber,extendsfromtheconnectortowithin5mmofthetip,providingstiffnessandadditionalinsulationtotheelectrodeshaft .Kapton-cladelectrodesarerecom-mendedwhentheelectrodeistobeinsertedthroughacannulaforextradeeppenetration .

#5468

#5470 (mount in M3301EH holder, see page 200)

Electrode plugged into adapter #5470

#5469

Gold-plated pins (#5482) and sockets (#5483) may be attached to 24-, 26-, or 28-gauge wire.

Heat Treated Tip(above)isidealforpenetraingtoughmembranes(notrecommendedforchronicimplantation) .ThisprocessisperformedusingamicroforgeinwhichtheheatingelementispositionedincloseproximitytothetipinordertomelttheParylene-Cdistaltotheexposedmetal .ItprovidesasmoothtransitionandproducesbetteradherenceoftheParylene-Ctothemetal .

Tohaveyourelectrodesheattreated,justaddthesuffix“H”toanyofthe“KT”numbersonthefacingpage .Costofthetreatmentis$10perpackageof10electrodes($20perpackageforTypeB) .

Type A

Type B

Type C

Type D

stainless steel tubing Polyimide tubing

exposed stainless steel surface

core conductor

shrink tubing

Wx

L=3" or 5" (2" for PTM)Y

Y <0.4 mm

Note: Electrode diagrams not shown to scale.

Insulated metal conductor with exposed concentric surface

#300102

Metal Exposed tip25:1 taper

Parylene-C*

.404"(10.26 mm)

.389"(9.88 mm)

.031"(0.79 mm)

0.51 mm ID

pin #5482

socket #5483



ACCESSORIES300102 Micromanipulatorholder,4in .,2mmto0 .031socket5468 2mmreceptacleto0 .031-inchjack(forOmega-TipZ)5469 Adaptsminibananaplug(DAM80)to0 .031-inch receptacle(metalmicroelectrode)5470 0 .031-inchjack,28ga .wire,12inch(pkgof4)5482* Pins,0 .031-inch,gold-plated(pkgof50)5483* Sockets,0 .031-inchgold-plated(pkgof50)

*Gold-plated pins (#5482) and sockets (#5483) may be attached to 24-, 26-, or 28-gauge wire.



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2525Prices shown are in U.S. dollars. Actual charges will vary because of import duty, freight, and currency fluctuations. To obtain an exact quotation, contact your WPI office.


Introductory AssortmentsEach of these assortment kits includes electrodes with different impedance within each style. Use an assortment kit to determine which electrode you need for your experiment. Ten electrodes per box, no mixing. See next page for electrode nomenclature.

Item Contains the following electrode impedances by quantity (pkg of 10) PriceTM31/33Axx TM33A05(2),TM33A10(3),TM33A20(3),TM31A50(2) US$170TM31/33AxxKT TM33A05KT(2),TM33A10KT(3),TM33A20KT(3),TM31A50KT(2) US$229TM33Bxx TM33B01(2),TM33B05(2),TM33B10(3),TM33B20(3) US$170TM33BxxKT TM33B01KT(3),TM33B05KT(2),TM33B10KT(3),TM33B20KT(2) US$229TM53Axx TM53A05(3),TM53A10(3),TM53A20(4) US$177TM53AxxKT TM53A05KT(3),TM53A10KT(3),TM53A20KT(4) US$265TST33AxKT TST33A05KT(3),TST33A10KT(4),TST33A20KT(3) US$476TST53AxKT TST53A05KT(3),TST53A10KT(4),TST53A20KT(3) US$518PTM23Bxx PTM23B05(3),PTM23B10(3),PTM23B20(4) US$382PTM23BxxKT PTM23B05KT(4),PTM23B10KT(3),PTM23B20KT(3) US$437

Selection Guide for Metal ElectrodesItem Length Insul . Shaft Nominal Tip Diam . Typical Use Price Thick . Diam . Impedance (± 20%)Platinum Iridium — Profile A Package of 10 PTM23B05 51mm 3µ 0 .254mm 0 .5MΩ 1-2µ Singleandmultiunitrecording,stimulation,Chronicimplants US$382PTM23B05KT 51mm 3µ 0 .356mm 0 .5MΩ 1-2µ Singleandmultiunitrecording,stimulation,chronicimplants US$437PTM23B10 51mm 3µ 0 .254mm 1 .0MΩ 1-2µ Singleandmultiunitrecording,stimulation,Chronicimplants US$382PTM23B10KT 51mm 3µ 0 .356mm 1 .0MΩ 1-2µ Singleandmultiunitrecording,stimulation,chronicimplants US$465PTM23B20 51mm 3µ 0 .254mm 2 .0MΩ 1-2µ Singleandmultiunitrecording,stimulation,Chronicimplants US$382PTM23B20KT 51mm 3µ 0 .356mm 2 .0MΩ 1-2µ Singleandmultiunitrecording,stimulation,chronicimplants US$465

Tungsten — Profile A Package of 10TM31A10 76mm 1µ 0 .127mm 1 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$177TM31A10KT 76mm 1µ 0 .216mm 1 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$242TM31A20 76mm 1µ 0 .127mm 2 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$177TM31A20KT 76mm 1µ 0 .216mm 2 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$242TM31A50 76mm 1µ 0 .127mm 5 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$177TM31A50KT 76mm 1µ 0 .216mm 5 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$242TM31C05 76mm 1µ 0 .085mm 0 .5MΩ 1µ Recordingfromsmalltightlypackedcells US$177TM31C05KT 76mm 1µ 0 .145mm 0 .5MΩ 1µ Recordingfromsmalltightlypackedcells US$242TM31C10 76mm 1µ 0 .085mm 1 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$177TM31C10KT 76mm 1µ 0 .145mm 1 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$242TM31C20 76mm 1µ 0 .085mm 2 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$177TM31C20KT 76mm 1µ 0 .145mm 2 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$242TM31C40 76mm 1µ 0 .085mm 4 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$177TM31C40KT 76mm 1µ 0 .145mm 4 .0MΩ 1µ Recordingfromsmalltightlypackedcells US$242TM33A05 76mm 3µ 0 .127mm 0 .5MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$170TM33A05KT 76mm 3µ 0 .216mm 0 .5MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$229TM33A10 76mm 3µ 0 .127mm 1 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$170TM33A10KT 76mm 3µ 0 .216mm 1 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$229TM33A20 76mm 3µ 0 .127mm 2 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$170TM33A20KT 76mm 3µ 0 .216mm 2 .0MΩ 1µ Multiunitandsingleunitrecordingandmicrostimulation US$229TM33B01 76mm 3µ 0 .254mm 0 .1MΩ 1-2µ Singleandmultiunitrecordingandmicrostimulation US$170TM33B01KT 76mm 3µ 0 .356mm 0 .1Ω 1-2µ Singleandmultiunitrecordingandmicrostimulation US$229TM33B05 76mm 3µ 0 .254mm 0 .5MΩ 1-2µ Singleandmultiunitrecordingandmicrostimulation US$170TM33B05KT 76mm 3µ 0 .356mm 0 .5Ω 1-2µ Singleandmultiunitrecordingandmicrostimulation US$229TM33B10 76mm 3µ 0 .254mm 1 .0MΩ 1-2µ Singleandmultiunitrecordingandmicrostimulation US$170TM33B10KT 76mm 3µ 0 .356mm 1 .0Ω 1-2µ Singleandmultiunitrecordingandmicrostimulation US$229TM33B20 76mm 3µ 0 .254mm 2 .0MΩ 1-2µ Singleandmultiunitrecordingandmicrostimulation US$170TM33B20KT 76mm 3µ 0 .356mm 2 .0Ω 1-2µ Singleandmultiunitrecordingandmicrostimulation US$229TM33C05 76mm 1µ 0 .085mm 0 .5MΩ 1µ Singleunitandstim/chronicuse US$177

Concentric Electrodes*Item Metal Core Length Imp Probe Outer Tip Core diam. Y dim. X dim. w/ polyimide Price Diameter (total) Diam. (pkg of 5)

TM33CCNON tungsten 3”(76) 10-15K 0.013”uninsulated(325µm) 3-4µ .003”(76µm) 0.4mm .005”(127µm) US$265TM53CCNON tungsten 5”(127) 10-15K 0.014”uninsulated(350µm) 3-4µ 005”(127µm) 0.4mm .008”(203µm) US$299TM33CCINS tungsten 3”(76) 10-15K 0.016”insulated(400µm) 3-4µ 003”(76µm) 0.4mm .005”(127µm) US$288TM53CCINS tungsten 5”(127) 10-15K 0.018”insulated(450µm) 3-4µ 005”(127µm) 0.4mm .008”(203µm) US$322PTM23CC001NON Pt/ir 2”(51) 10K 0.020”uninsulated(525µm) 3-4µ 0.01”(254µm) 0.4mm .014”(356µm) US$380PTM23CC001INS Pt/ir 2”(51) 10K 0.029”insulated(760µm) 3-4µ 0.01”(254µm) 0.4mm .014”(356µm) US$380PTM3CC02INS Pt/irnsfine 3”(76) 200K 0.013”insulated(325µm) 2-4µ 0.002”(50.8µm) .25mm .004”(114µm) US$403*All have a stainless steel outer shaft

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TM33C05KT 76mm 1µ 0 .145mm 0 .5MΩ 1µ Singleunitandstim/chronicuse US$242TM33C10 76mm 1µ 0 .085mm 1 .0MΩ 1µ Singleunitandstim/chronicuse US$177TM33C10KT 76mm 1µ 0 .145mm 1 .0MΩ 1µ Singleunitandstim/chronicuse US$242TM33C20 76mm 1µ 0 .085mm 2 .0MΩ 1µ Singleunitandstim/chronicuse US$177TM33C20KT 76mm 1µ 0 .145mm 2 .0MΩ 1µ Singleunitandstim/chronicuse US$242TM33C40 76mm 1µ 0 .085mm 4 .0MΩ 1µ Singleunitandstim/chronicuse US$177TM33C40KT 76mm 1µ 0 .145mm 4 .0MΩ 1µ Singleunitandstim/chronicuse US$242TM53A05 127mm 3µ 0 .127mm 0 .5MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$196TM53A05KT 127mm 3µ 0 .216mm 0 .5MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$292TM53A10 127mm 3µ 0 .127mm 1 .0MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$216TM53A10KT 127mm 3µ 0 .216mm 1 .0MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$265TM53A15 127mm 3µ 0 .127mm 1 .5MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$177TM53A15KT 127mm 3µ 0 .216mm 1 .5MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$242TM53A20 127mm 3µ 0 .127mm 2 .0MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$196TM53A20KT 127mm 3µ 0 .216mm 2 .0MΩ 2µ Deeppenetrationsforsingleandmultiunitrecordingandstimulation US$265TM53B05 127mm 3µ 0 .254mm 0 .5MΩ 1µ Deeppenetrations US$196TM53B05KT 127mm 3µ 0 .356mm 0 .5MΩ 1µ Deeppenetrations US$242TM53B10 127mm 3µ 0 .254mm 1 .0MΩ 1µ Deeppenetrations US$196TM53B10KT 127mm 3µ 0 .356mm 1 .0MΩ 1µ Deeppenetrations US$242TM53B20 127mm 3µ 0 .254mm 2 .0MΩ 1µ Deeppenetrations US$196TM53B20KT 127mm 3µ 0 .356mm 2 .0MΩ 1µ Deeppenetrations US$242

Elgiloy®/Stainless Profile A Package of 10 SSM33A05 76mm 3µ 0 .229mm 0 .5MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$154SSM33A05KT 76mm 3µ 0 .356mm 0 .5MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$229SSM33A10 76mm 3µ 0 .229mm 1 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$154SSM33A10KT 76mm 3µ 0 .356mm 1 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$229SSM33A20 76mm 3µ 0 .229mm 2 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$170SSM33A20KT 76mm 3µ 0 .356mm 1 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$230SSM33A70 76mm 3µ 0 .229mm 7 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$177SSM33A120 76mm 3µ 0 .229mm 12 .0MΩ 1-2µ RecordingandStimulating(Prussianbluestaining) US$177

Tungsten Profile B Package of 10 TST33A05KT 76mm 3µ 0 .356mm 0 .5MΩ 1µ Stereotrode/Bipolar,differentialmeasurements US$481TST33A10KT 76mm 3µ 0 .356mm 1 .0MΩ 1µ Stereotrode/Bipolar,differentialmeasurements US$481TST33A20KT 76mm 3µ 0 .356mm 2 .0MΩ 1µ Stereotrode/Bipolar,differentialmeasurements US$478TST33C05KT 76mm 3µ 0 .216mm 0 .5MΩ 1µ Stereotrode/Bipolar,differentialmeasurements—extrafine US$495TST33C10KT 76mm 3µ 0 .216mm 1 .0MΩ 1µ Stereotrode/Bipolar,differentialmeasurements—extrafine US$495TST33C20KT 76mm 3µ 0 .216mm 2 .0MΩ 1µ Stereotrode/Bipolar,differentialmeasurements—extrafine US$495TST53A05KT 127mm 3µ 0 .356mm 0 .5MΩ 1-2µ Stereotrode/Bipolar,differentialmeasurements US$497TST53A10KT 127mm 3µ 0 .356mm 1 .0MΩ 1-2µ Stereotrode/Bipolar,differentialmeasurements US$497TST53A20KT 127mm 3µ 0 .356mm 2 .0MΩ 1-2µ Stereotrode/Bipolar,differentialmeasurements US$497

Pure Iridium Profile C Package of 5 IRM23E01 50mm 3µ 0 .106mm 0 .1MΩ 2-3µ Multiunit&ERPrecording&stimulation US$454IRM23E01KT 50mm 3µ 0 .180mm 0 .1MΩ 2-3µ Multiunit&ERPrecording&stimulation US$500IRM23E05 50mm 3µ 0 .106mm 0 .5MΩ 2-3µ Singleandmultiunitrecordingandstimulation US$454IRM23E05KT 50mm 3µ 0 .180mm 0 .5MΩ 2-3µ Singleandmultiunitrecordingandstimulation US$500IRM23E10 50mm 3µ 0 .106mm 1 .0MΩ 1-2µ Singleandmultiunitrecordingandstimulation US$454IRM23E10KT 50mm 3µ 0 .180mm 1 .0MΩ 1-2µ Singleandmultiunitrecordingandstimulation US$500IRM23E15 50mm 3µ 0 .106mm 1 .5MΩ 1-2µ Singleandmultiunitrecordingandstimulation US$454IRM23E15KT 50mm 3µ 0 .180mm 1 .5MΩ 1-2µ Singleandmultiunitrecordingandstimulation US$500IRM23E20 50mm 3µ 0 .106mm 2 .0MΩ 1-2µ Greaterselectivity&microstimulation US$454IRM23E20KT 50mm 3µ 0 .180mm 2 .0MΩ 1-2µ Greaterselectivity&microstimulation US$500IRM23E25 50mm 3µ 0 .106mm 2 .5MΩ 1-2µ Greaterselectivity-smallcells US$454IRM23E25KT 50mm 3µ 0 .180mm 2 .5MΩ 1-2µ Greaterselectivity-smallcells US$500IRM23E30 50mm 3µ 0 .106mm 3 .0MΩ 1-2µ Greaterselectivity-smallcells US$454IRM23E30KT 50mm 3µ 0 .180mm 3 .0MΩ 1-2µ Greaterselectivity-smallcells US$500IRM23E50 50mm 3µ 0 .106mm 5 .0MΩ 1-2µ Greaterselectivity-smallcells US$454IRM23E50KT 50mm 3µ 0 .180mm 5 .0MΩ 1-2µ Greaterselectivity-smallcells US$500

Platinum Iridium Profile D Package of 5 PTM123B05KT305mm 3µ 0 .61mm 0 .5MΩ 1-2µ Deepbrainstudies US$469PTM123B10KT 305mm 3µ 0 .61mm 1 .0MΩ 1-2µ Deepbrainstudies US$469PTM123B20KT305mm 3µ 0 .61mm 2 .0MΩ 1-2µ Deepbrainstudies US$469

Tungsten Profile D Package of 5 TM123A05KT 305mm 3µ 0 .406mm 0 .5MΩ 1µ Deepbrainstudies US$409TM123A10KT 305mm 3µ 0 .406mm 1 .0MΩ 1µ Deepbrainstudies US$409TM123A20KT 305mm 3µ 0 .406mm 2 .0MΩ 1µ Deepbrainstudies US$409

Elgiloy®/Stainless Profile D Package of 5 SSM123B05KT 305mm 3µ 0 .61mm 0 .5MΩ 1-2µ Deepbrainstudies US$373SSM123B10KT 305mm 3µ 0 .61mm 1 .0MΩ 1-2µ Deepbrainstudies US$373SSM123B20KT 305mm 3µ 0 .61mm 2 .0MΩ 1-2µ Deepbrainstudies US$373

EligoySteel*Cobalt/chromium/nickelalloy .ThekTsuffixreferstoKapton™cladding .AllMetalMicroelectrodesareavailableincustomlengths,bluntorheattreaded(extracharge) .

Ordering:AddtheBsuffixwherebluntelectrodesaredesired .(Forexample,anIRM123A10KTorderedasabluntwillbeIRM123A10KTB .)AddtheHsuffixwhereheattreatedelectrodesaredesired .(Forexample,anIRM123A10KTorderedaswithheattreatmentwillbeIRM123A10KTH .)



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ΩΩmega-Tip-Z™

OMEGA-TIP-Z SPECIFICATIONS INPUTRESISTANCE 1012Ω,typicalINPUTLEAKAGECURRENT 1pA,typicalMAX .INPUTVOLTAGESWING ±2VVOLTMETER Range 0to±2000mVDC Accuracy ±0 .1%ofreading,±1leastsignificantdigit Resolution 1mV(3 .5digits)OHMMETER Range 0to20MΩ(metal) 0to200MΩor0to2000MΩ(glass) Measurement 10nAand1nA(500Hzsquarewave) Resolution 10KΩ Accuracy ±20%PROBE 1 .2cmdiameter×3 .2cmlongPROBEHANDLE 4 .7mmdiameter×8 .9cmlongPROBECABLE 1 .5mMAINHOUSING 7×4×2in .(18×10×5cm)POWER 6AA1 .5-voltalkalinecells,suppliedSHIPPINGWEIGHT 4lb(1 .8kg)

Millivolt and megohm meter measures impedance of metal or glass capillary microelectrodesOmega-Tip-Zwascreatedespeciallyformeasuringimpedanceinetchedtungsten,platinum-iridium*andsteelmicroelectrodes,aswellaselectrolyte-filledmicropipettes .Themeter’sACimpedance-measuringcircuitisunaffectedbyelectrodeoffsetortipjunctionpotentials .Thegold-platedminiatureprobeletsyouconvenientlymonitormicro-electrodeimpedanceinelectrolytes,andanelectrodetipcleaningfeatureletsyouremovebuildupquickly .Omega-Tip-ZcanalsomeasureDCelectrodetippotentialsupto2000millivolts .Theinstrumentoperatesforhundredsofhourswithoutbatteryfailure .

NOTE: Metalmicroelectrodeswhichhavebeenprecalibratedat1kHzshouldbebaselinedforusewithOmega-Tip-Z .

*See Metal Microelectrodes, page 24.

Rodent Brain Matrices

WPIoffersoneofthelargestselectionsofbrainmatricesavailable .Madeofacrylic,orstainlesssteel,these

matricesaresturdyandcanbeheated,chilled,autoclaved(stainlesssteelonly),scrubbed

—andstanduptorigorousdailyuse .Coronalmatriceshavetheadditionalfeatureofamid-linesagittalcuttofacilitatesplittingoftheleftandrighthemispheres .Sectionscanbeasfineas1-mm .Theolfactory/spinal/notchiscutintoeachmatrix .

• Large species selection• From mouse to rat• Repeatable sections to 1 mm• Can be chilled and sterilized• Available in acrylic or stainless steel



OMEGA-Z Ωmega-Tip-ZwithProbe&Holder711P ReplacementProbe5468 Adaptertoconnectmetalmicroelectrodesto probe,2mmsocketto .031in .receptacle

OPTIONAL ACCESSORIESNOVA NovaflexFiberOpticIlluminator(115v,60HZ)NOVA-Z NovaflexFiberOpticIlluminator(230v,80HZ)500186 BifurcatedLightGuidewithlensesNOVA-186 NovaflexIlluminatorandbifurcatedlightguide

RBMA-200C Adultmouse,coronalslices,acrylicRBMA-200S Adultmouse,sagittalslices,acrylicRBMA-300C Rat,175-300g,coronalslices,acrylicRBMA-300S Rat,175-300g,sagittalslices,acrylicRBMA-600C Rat,300g-600g,coronalslices,acrylicRBMA-600S Rat,300g-600g,sagittalslices,acrylicRBMS-200C Adultmouse,coronalslices,stainlesssteelRBMS-200S Adultmouse,sagittalslices,stainlesssteelRBMS-300C Rat,175-300g,coronalslices,stainlesssteelRBMS-300S Rat,175-300g,sagittalslices,stainlesssteelRBMS-600C Rat,300g-600g,coronalslices,stainlesssteelRBMS-600S Rat,300g-600g,sagittalslices,stainlesssteelBLADES Single-EdgeBlades(pkgof100)

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Fire Polishing

Tip Reducing (holding pipettes)

Tip SealingLarge Tip Sharpening (contact stretching)

Tip Bending Carbon Fiber Sealing in Plastic Sensor

Filament Holder mounts directly to objective to provide precise control of heating element position.

Microforging, Micropipette Calibration and Microinjection — in a single device!

ThelowheatcapacityandlowthermalcoefficientoflinearexpansionofthefilamentsarekeydesignfeaturesoftheDMF1000 .Thelowheatcapacityofthefilamentallowsittoreachfire-polishingtemperatureswithoutexcessiveheat .Thispermitstheusertobringthepipettetipclosetothefilamentduringpolishingwithoutfearofcollapsingthepipettetip .Lowheatcapacityeliminatestheneedforanauxiliaryair-coolingsystem .Thelowcoefficientofexpansioncharacteristicofthefilamentensuresminimaldisplacementofthefilamentduringheating .Thisfeatureeliminatesmuchoftheguessworkoutoftipplacementinrelationtothefilament .

TwodifferentheatingfilamentsareprovidedwiththeDMF1000toaccommodatevariousapplications .TheH5filamentislargegaugeandcanbereformedintoa“U”forfabricationofpipettes,airformingofpatchpipettesandotherapplications .TheH4isasmallergaugefilamentandisidealforpolishingpatchclamppipettes .

The Pipette and Microscope StageThepipetterestsonaspeciallydesignedholderthatsitsontopofthemicroscopestage .Thepositionofthepipette,relativetotheheatingfilament,iscontrolledbythe(X,Y,Z)adjustmentofthestage .Thisuniquedesignmakeslocatingandpolishingthepipetteextremelyeasy .Thestageofthemicroscopehasahighqualityrailthatgivesprecise,smoothandstablecontrolofthepipettesmovement .Thisconfigurationalsoeliminatestheneedandexpenseofanadditionalmicromanipulatortocontrolpipettemovement .

TheDMF1000isa‘state-of-the-art’microprocessor-controlledmicroforgeofferingunmatchedperformance .Designedforfabricationofbothsmallpatchclampglasspipettesandlargerinjectionpipettes,theDMF1000shouldfindmanyusesinthelaboratory .TheDMF1000isbasedonadesignsimilartothatfirstusedinWPI’sextremelypopularmicroforgemodel,theMF200 .TheextensiveimprovementsincorporatedintotheDMF1000greatlyincreaseitsversatilityandperformance,makingitoneofthemostpowerfulmicroforgesonthemarket .

Digital Signal Processor (DSP) TechnologyTheDMF1000ispoweredbythelatestdigitalsignalprocessor(DSP)technology .Adigitaltimerisusedtopreciselycontrolthepolishheatingtime .Tenmemoriescanbeusedtostoresettingsoftheheatingpowerandheatingduration .Allofthesettingsarecontrolledanddisplayeddigitallyforbetteraccuracyandreproducibility .Twodifferentoperatingmodesareprovided:ManualandAuto .IntheManualmode,theDSPwillmemorizethedurationofthetimethatisusedtoachieveadesiredpolishing .InAutomode,theheatwillbeappliedforthedurationofthetimersetting .

Unique Features of the DMF1000 SystemTheDMF1000systemincludesaspeciallyconfiguredWPImodelH602researchgradecompoundmicroscopeequippedwithahighqualitymetallurgic40xlong-workingdistanceobjectiveandapairof10xeyepieces(a15xoptioneyepieceisalsoavailable) .Itisthemostpowerfullong-workingdistanceobjectivecurrentlyavailableonanycommercialmicroforge .Thelongworkingdistanceobjectivereducesthedangerofdamagetotheobjectivelensduringtheheatingprocess .

OtherbenefitsoftheDMF1000designincludetheuseofaKohlerilluminatorandAbbecondenser,whichprovidethereducedglareandsharperimagecontrastnecessarywhenpolishingpipettesassmallashalfamicron(0 .5µm)indiameter .

Pressure PolishingTheDMF1000incorporatesauniquedigitalpneumaticpressurefeaturethatenablespressurizedairtobedeliveredthroughthepipetteduringfirepolishing .Inthefabricationofpatchpipettes,thepressurizedaircanbeusedtobluntthetaperatthepipettetipwith-outchangingthesizeofthetipopening .Thisreduces

electricalresistanceofthetip,leadingtolowernoiseduringpatch-clamprecordings(Goodman&Lockery,2000) .

Ease of useThe Heating FilamentWithaconventionalmicroforgeoftenthemostdifficultandtime-consumingpartofusingahighmagnificationobjectiveisbeingabletomoveboththeheatingfilamentandthepipetteintothesameviewingarea .Findingandmovingboththeheatingfilamentandthepipettewithoutcollisioncanbeachallenge .However,thisdifficultyiseliminatedwiththeDMF1000becausetheheatingfilamentisdirectlyattachedtothemicroscope’sobjective .Henceitcanbeeasilyadjustedtoanypositionwithintheviewingarea .

DMF1000





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Typical applications of the DMF1000

Polishing the Patch PipettesItiswellknownthattheproperfirepolishingofpatchpipettesisthesinglemostimportantfactorforformingastablegiga-sealinpatchclamprecording .Thisisevenmoreimportantthanthetypeofglasscapillaryused .Difficultiesoftenariseinforminggiga-sealsbecausethepolishingofpatchpipettesusingaconventionallowmagnificationmicroforgeisinadequate .However,sincetheDMF1000usesa40Xlong-workingdistanceobjective,pipettepolishingismuchmoreaccuratelycontrolled .PipettespolishedusingtheDMF1000achieveexcellentstablegiga-sealswithawidevarietyofcells .BothwholecellpatchpipettesandsinglechannelpatchpipettescanbeconvenientlypolishedwiththeDMF1000tothehighestqualityandreproducibilityachievablewithanymicroforge .

Forthesingle-channelpatchclamppipettesthepipetteneedstobepre-coatedwithSylgard184beforepolishing .Forthisproceduretheusercanfollowasimpleandeffectivecoatingmethoddescribedpreviously(Li,1999)

Microforging Holding PipettesAholdingpipettewithalargeblunttipandasmallopeningisusedtoholdafloatingcellinplacepriortomicroinjectionbyapplyingsuctiontotherearofthepipette .Theprocedureformakingholdingpipettesinvolvesthreesteps:squaringoff,largeboreflamepolishing,andtipreducing .Thesestepsareaccomplishedwithalargerheatingfilament .

Microforging Beveled Injection PipettesOccasionally,abeveledlargeborepipetteisnotsharpenoughtopenetrateacellwithoutdamagingtheareaaroundthepipette .WiththeDMF1000andthelargeheatingfilament,asharppointcanbeformedonthebeveledtiptoassistthepenetrationofthecell .Thisprocessisreferredtoascontactstretching .

Pipette Tip Calibration & MicroinjectionTheintegrateddigitalpneumaticpressuresystemcanbeusedtocalibratetheprecisediameter(I .D .)ofamicropipettetip,basedonatechniquedescribedpreviously(Hagag&Randolph1990,Bowman&Ruknudin1999) .Thepressuresystemcanalsobeusedseparatelyasasimplebuthighlyaccuratecontrollerformicroinjectionapplications .

DMF1000 SPECIFICATIONSACPOWERMODULE 100-240VAC50/60HzTIMERRANGE(forheater&timer) 0 .01to360secNUMBEROFMEMORYS 10PRESSUREADjUSTINGRANGE 0 .5–60PSI(3 .5–414kPaPRESSURERESOLUTION 0 .1PSI(0 .7kPa)FILAMENTS H4—Smallfilamentforworkingwith40× longworkingdistanceobjective . H5—Largefilamentforworkingwith10× objective .Filamentadjustmentassembly providedforbothobjectives .HEATERANDTIMERCONTROL AutoorManualviaPushbutton,TTL, orOptionalFootswitch .OBjECTIVE 40×Long-WorkingDistance(3mm)EYEPIECE 10×(pair) 15×(pair)optional RETICLE(10xeyepieceonly) 1 .25µm/division(at40×) 0–90ºangleat5º/divisionDIMENSIONS:ControlUnit 10 .2×17 .8×4 .8cm(4×7×17⁄8in .)SHIPPINGWEIGHT 1 .8kg(4lb .)MICROSCOPE SeeH602,page209SHIPPINGWEIGHT 7 .3kg(16lb .)

H602 1000× Compound Microscope

● Coaxial coarse and fine focusing control, including a locking mechanism.

● Spring-loaded achromatic objectives prevent broken slides.

● Centerable Abbe condenser.

● 20-Watt solid state Kohler illuminator with variable lamp intensity control.

● Total magnification of 1000x.

● Stage is graduated in both x- and y-axes.

H602 SPECIFICATIONSEYEPIECES 10×Widefield,18mmfieldCONDENSER CenterableAbbe,1 .25NAILLUMINATOR Kohler,6V20-watthalogen

POWER95-135Vor220-240V,50/60Hz(Specify line voltage & power cord.)

SHIPPINGWEIGHT 16lb(7 .3kg)OBjECTIVES 4X0 .1NAAchromaticWD35 .8mm

10X0 .25NAAchromaticWD6 .81mm40X0 .65NAAchromaticWD0 .62mm100Xoil1 .25NAAchromaticWD0 .35mm



DMF1000-1 CompleteMicroforge,inclh602stereoMicroscope(110v)DMF1000-2 CompleteMicroforge,inclh602stereoMicroscope(220v)DMF1000-M1 Microforgewithoutmicroscope(including40xlong workingdistanceobjective)(110v)DMF1000-M2 Microforgewithoutmicroscope(including40xlong workingdistanceobjective)(220v)

OPTIONAL ACCESSORIES500292 optional15xeyepieces(pair)forh600seriesMicroscopes

Note: No reticle for 15x eyepiecesDMF1000-H5 Replacementheatingfilament(largegauge)MF200-H4 Replacementheatingfilament(smallgauge)13142 OptionalfootswitchDMF-UPGRADE ConvertsMF200toDMF1000

Includes new metallurgical 40x objective

H602 1000×BinocularMicroscope(120V)H602-240 1000xBinocularMicroscope(240V)

OPTIONAL ACCESSORIES14253 ReplacementLamp,20W6V(pkgof5)500292 Optional15xEyepiece(pair)500329 25× Long-WorkingDistanceObjective,5mm0 .50NA800292 40× Long-WorkingDistanceObjective,3mm0 .25NA

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MF200 Microforge

MICROFORGE FEATURE COMPARISON MF200 DMF1000H602 Microscope ✔ ✔40x Long Working Distance Objective ✔ ✔Analog Controller ✔ ✔Digital Controller — ✔Pressurized Air Control — ✔Microinjection Capability — ✔Optional foot Switch ✔ ✔Memory — ✔Auto-sense of filament Type — ✔Digital Temperature Control — ✔

MF200 SPECIFICATIONSACPOWERMODULE 100-240VAC50/60Hz

FILAMENTS(3) H2,H3,H4

FILAMENTON PushbuttonControlledorOptionalFoot SwitchControlled

FILAMENTADjUSTMENTASSEMBLY For40×and25×Long-Working DistanceObjectives:mountsonobjective

OPTIONAL FilamentHolderforstandard10×Objective (ModelH602microscope)

OBjECTIVE 40×Long-WorkingDistance(3mm)

OPTIONAL 25×Long-WorkingDistance(5mm)

EYEPIECE 10×(pair)

RETICLE(10×eyepieceonly) 1 .25µm/division(at40×) 0-90ºAngleat5º/division

OPTIONALEYEPIECE 15×(pair)

GLASSHOLDER MountsonMicroscopeStage

DIMENSIONS:ControlUnit 10 .2×17 .8×4 .8cm(4×7×17⁄8in .)

SHIPPINGWEIGHT 1 .4kg(3lb .)

MICROSCOPE See H602

SHIPPINGWEIGHT 7 .3kg(16lb .)

TheMF200Microforgeisaversatileinstrumentdesignedspecificallyforthefabricationofglassmicropipettesandotherrelatedtools .ThesystemwasdevelopedincollaborationwithDr .MingLioftheDepartmentofPharmacology,UniversityofSouthAlabama .Itisperfectforpatchpipettetippolishing,tipsizereduction,contactstretching,in vitrofertilizationpipetteproductionandavarietyofotherpipetteconfigurations .TheMF200simple,reliableandispricedeconomically .

Features of the MF200TheMF200systemincludes:Aneasytouseanalogtemperaturecontroller,aspeciallyconfiguredWPImodelH602researchgradecompoundmicroscope,40xlong-workingdistanceobjectiveand10xeyepiece .40xmagnificationisessentialwhenpolishingpipettesassmallashalfamicron(0 .5µm)indiameter .Comparedtoaconventional40xobjective,thelongworkingdistanceobjectivereducesthedangerofdamagetothepipetteand/orobjectivelensduringthepolishingprocess .ItisalsotheonlycommercialmicroforgeusingtheKohlerilluminatorandAbbecondenserforillumination .Thisprovideslessglareandsharperimageofthepipettethanfrostedglassilluminator,whichwasusedonalloftheothercommercialMicroforge .

Sometimes the simplest designs work best .



MF200-1 CompleteMicroforgesystem,incl.h602Microscope(110v)MF200-2 CompleteMicroforgesystem,incl.h602Microscope(220v)MF200-M1 MF200withoutmicroscope(110v)MF200-M2 MF200withoutmicroscope(220v)500292 optional15×eyepieces(pair)forh600seriesMicroscopes

Note: No reticle available for 15× eyepieces500329 25×long-workingdistanceobjective (fits most microscopes with a 160 mm Focal Length) MF200-H2 Replacementheatingfilament(largegauge)MF200-H3 Replacementheatingfilament(mediumgauge)MF200-H4 Replacementheatingfilament(smallgauge)75070 FilamentAdjustmentAssemblyfor10×StandardObjective (fits microscope model H602 only)13142 Optionalfootswitch



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The only microbeveler system with Guided Light!

TheadvantageofWPI’sMicroBeveleroverothertypesofsolid-surfacebevelersisthattheabrasivesurfacecanbeeasilyrefreshed .Insteadofusingaconventionalsolidabrasivedisk,theMicroBevelerabrasivesurfaceismadeofahighqualitylappingfilm,widelyusedinthefiberopticsindustry .Whenthesurfaceisdamagedorloadedupwithglassparticles,theabrasivefilmcanbeeasilyreplaced .

ThesolidpolishingsurfaceofWPI’snewMicroBeveler,turningat6,000rpm,providessufficientcuttingforceforaverysharptipinaveryshorttime .Thecuttingsurfaceisveryflatandturnsverysmoothly,ensuringanundamagedtip .

Bevel micropipette tips larger than 1 micron, for applications such as microinjection

48000 SPECIFICATIONSBEVELINGSURFACE 3 .5inchdiameterdisk

ABRASIVEMATERIAL alumina,diamond

SPEEDOFROTATION 6000rpm

MOTOR ReversibleDirection

POWERREqUIREMENTS 120volts,60Hzor 240volts,50Hz,20VA tosuppliedpowersupply

DIMENSIONS BasePlate 22×28×1cm(8 .7×11×0 .4in .) OverallHeight 8cm(3in .)

SHIPPINGWEIGHT(48000) 16lbs .(7 .6kg .)

SHIPPINGWEIGHT(MBS) 35lbs .(16Kg .)

● Tool holder on microscope keeps pipette in focus during beveling

● Steel base provides solid support for beveler and other magnetic stands

● Stereo zoom microscope PZMIII Included in MBS system

● Variable speed, reversible

● Abrasive surface can be easily replaced by several types of Diamond and Alumina Lapping Film

● Pipette tip illuminated internally via Fiber optic illuminators

System now includes PZMIII

stereo microscope



MBS MicroBevelerSystem

Includes all components pictured above: 48000 MicroBeveler, NOVA illuminator, fiber optic cable, PZMIII Stereo Zoom Microscope with tilting base especially adapted for use with MicroBeveler, tool

holder, and pipette holder FOIMPH.

48000 MicroBeveler


OPTIONAL ACCESSORIES48015-03 LappingFilm,Alumina,0 .3micron(50-pack)48015-10 LappingFilm,Alumina,1micron(50-pack)48015-30 LappingFilm,Alumina,3microns(50-pack)48014-01 LappingFilm,Diamond,0 .1micron(3-pack)48014-05 LappingFilm,Diamond,0 .5micron(3-pack)48014-10 LappingFilm,Diamond,1micron(3-pack)48014-30 LappingFilm,Diamond,3microns(3-pack)48025 FiberOpticCableforPipetteIllumination 15934 ReplacementBevelerDiskPlate48300 TiltBaseAssemblyforPZMIIIbinocularhead48200 PZMToolHolder

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1300M SPECIFICATIONSBEVELINGSURFACE 7 .8cmdiameter,opticallyflatreflectiveglass

MAXIMUMBEVEL 0 .5µ,I .D .ALUMINAABRASIVEPOWDER 0 .05µsizesupplied(0 .3µalsoavailable)RPM 60rpmat120V,60Hz;50rpmat240V,50HzMOTOR ACsynchronousPOWERREqUIREMENTS 95-135Vor220-240V,50/60HzDIMENSIONSSteelbaseplate 8 .5x11x0 .375in .(22x28x1cm)Overallheight 4in .(10cm)Heightofabrasivesurface 2 .75in .(7cm)abovebaseplateSHIPPINGWEIGHT 20lb(9 .1kg)

Anoptically-flatmirroredglassdisk,wettedwithanabrasiveslurry,spinsat60rpm(120V),producingsharplybeveledtipsonfluid-filledglassmicroelectrodesofonemicronorsmaller .Thiseasescellimpalementandimprovestheelectrode'slinearity .Themicroelectrode’sresistancecanbemonitoredduringbevelingwithWPI’sΩmega-Tip-Z™megohmmeter(seepage31) .Thebevelerispermanentlymountedonaprecisionmagneticplatethatgivesstablesupportfortheoptional1350MMicropositionershown .Start-upkitincludes0 .05µmaluminaabrasivepowder#3531,“O”ring,wickelectrode,andwicksupport .

Easier cell impalement results in less damage and longer cell life

Model 1350M Micropositioner —Thispackage(shownwithbevelerabove)includesWPI’sM3301RManipulatorandanM10magneticstand .Thestand-manipulatorassemblymountsdirectlyontothebevelerbaseplate,allowingconvenientpositioningofelectrodesontothebevelingsurface .Threeaxesofadjustment,includingcoarseandfinecontrolintheaxisoftheelectrode .

Glass Micropipette Beveler for submicron tips



1300M MicroelectrodeBeveler&Start-UpKit(micropositioner not included)

Specify line voltage.

OPTIONAL ACCESSORIES2478 ReplacementMirroredDisk3531 AluminaAbrasive,0 .05µm(5g)fine3551 AluminaAbrasive,0 .30µm(5g)2479 Replacement“O”Ring1350M MicropositionerandMagneticStandOMEGA-Z Ωmega-Tip-ZwithProbe&Holder711P ReplacementProbe5468 Adaptertoconnectmetalmicroelectrodesto probe,2mmsocketto .031in .receptacleNOVA NovaflexFiberOpticIlluminator(115v,60HZ)NOVA-Z NovaflexFiberOpticIlluminator(230v,80HZ)500186 BifurcatedLightGuidewithlensesNOVA-186 NovaflexIlluminatorandbifurcatedlightguideMES MicroelectrodeBevelerSystem

MESincludes:1300MMicroelectrodeBeveler;1350MMicropositioner&MagneticStand;OmegaZ;5052SteelBasePlate;5468Adapter;M9

MagneticStand;3485KitRingstandMount .ShippingWeight:59lb .(27kg)



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●Doubletoppanelstructure .

●Automatic4-Barrelor7-BarrelPipettePuller .

●ExclusiveOptical-DigitalRulerMeasurement .

●ComputerizeReal-TimeFeedbackHeaterControl .

●PneumaticPullingForceandVeryCompactSize .

●ProgrammableandSavableSequencesforCreationandReproduction .

●ManufacturePresetPullingProgramsfor4and7-Barrel

●ThePMP-107hasallthefeaturesofthePMP-100Micropipettes .

Withjustprocessingonekey,thePMP-107ProgrammableMultipipettepullercansuccessfullypullaoneto7-barrelpipette .Equippedwithamicrocomputer,pneumaticpulingarm,pneumaticrotator,optical-digitalrulerandanewdesignedclamp,thePMP-107canautomaticallyheat,twistandpullamultibarrelpipette .Thereisnoneedforanymanualrotationoranyinconsistenttiminginterruptcontrol .Thewholepullingprocessingisprogrammableandundercontrolofapresetsequence .ThePMP-107isanewupgrademodelfromthePMP-100multipipettepuller .Therotation(twist)speedisadjustable .

Programmable Multipipette

Puller

PMP-107 SPECIFICATIONSPIPETTES Single-to7-Barrel

EACHBARRELO .D . 1mm(2mmforsingle-barrel)

PULLINGFORCE Pneumatic

HEATER Nichromecoilorfoil

HEATERCONTROL Microcontroller

HEATING 74generalheatlevels(24-99),64automaticheatlevels(45-98)

NUMBEROFSEqUENCES 25

STEPSPERSEqUENCE 18

TAPERLENGTHSETTING 0 .5-20mm

PRESSURE1 Adjustable0 .1-10psi

PRESSURE2 Adjustable0 .1-60psi

COOLINGPRESSURE Adjustableforrotationspeed

GASINPUTPRESSURE 30-60psi

ACTIONS Pull1,Pull2,Pull2/Cool,Rotation,CoolAir,Return

DISPLAY 20x4LCD

POWERINPUT 110/240VAC

POWERCONSUMPTION Maximum150watts

DIMENSIONS 14x11x7in .(35 .6x27 .9x17 .8cm)

WEIGHT 15lb(6 .8kg)

PMP-107



PMP-107 ProgrammableMultipipettePuller(110V)PMP-107-Z ProgrammableMultipipettePuller(240V)

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PUL-2isamicroprocessor-controlledtwo-stagepullerformakingglassmicropipettesormicroelectrodesusedinintracellularrecording,patchclampstudies,ormicroinjection .Itcanproducepipetteswithtipdiametersfromlessthan0 .1microntotensofmicrons .Heatsettingisdigitallycontrolledwith0 .1%accuracy .Pullinglengthcanbeadjustedtocontrolthetaperofthepipette .

Thistwo-stagepullerisacompact,versatile,reliableworkhorseataveryreasonablecost .ThemicroprocessorincombinationwithLED,digitaldisplayandsoundgeneratormakesthePUL-2easytouseandfoolproof .Controlsareintuitiveandself-explanatory .Everykeypadfunctionisconfirmedorrejectedwithanaudiblesignal .Thedigitaldisplayindicatesheatsettingor

thestatusofanoperation .AnLEDindicatorconfirmsthattheglasscapillaryisproperlyinstalledattheinitialpullingpositionforbetterreproducibility .

Two-stagepullingisautomaticallyaccomplishedaccordingtothemicroprocessorsetting,withnoneedofhandintervention .Heatsettingsforbothstagescanbestoredinmemory .Thepermanentmemorystoresupto99heatsettings .

Anotheruniquefeatureofthispulleristhehighqualityswitchingpowersupplythatnotonlyreducessizeandweightandcanbeusedanywhereintheworldwithoutworryaboutthelinevoltagedifferences,butalsosolvestheproblemofpullingreproducibilityduetolinevoltagefluctuation .Heatingvoltagecanbecontrolledwithin0 .1%accuracyevenwhenlinevoltagefluctuatesfrom90to240VAC .

PUL-2 SPECIFICATIONSHeaterElement Nickel-chromiumcoil

PullingForce Gravity,selectableat 250g&330g

Max .CapillaryLength 210mm

Min .CapillaryLength 55mm

Memory 99sets

Power 90-240V,50/60Hz .

Dimensions 21x21x20cm

ShippingWeight 15lb

Programmable two-stage micropipette puller

The importance of the multistage pullerAsingle-stagepullerisgoodonlyforproducingintracellularelectrodesthathavelessthan0 .1microntips .Whenthepullstarts,theglassistoosoftforalargertiptoform .Forpullingpipetteswithatiplargerthan0 .1micron,atwo(ormore)stagepullerisrequired .Thefirstpullingstageproducesathinsectiononthecapillary .Thepullingforceisthenmoreconcentratedonthethinsection,onwhichthestressisincreasedduringthesecondpullingstage .Withconcentratedforceduringsecondstagepulling,thecapillarycanbeseparatedatalowertemperature(i.e.,higherglassviscosity)toformalargertip .Bycontrollingthepullinglengthofthefirstpull,PUL-2canproducetaperssuitableformostapplications .



PUL-2 ProgrammableTwo-stageMicropipettePuller502194 HeatingCoil,Nickel-Chromium,forPUL-2(packageof5)



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PhysiologyCardiovascular

TBM4M Transbridge 4-channel Transducer Amplifier ...................................................................................................................................36

BP1 Blood Presure Monitor ................................................................................................................................................................................... 37

BLPR2 Blood Pressure Transducer ...................................................................................................................................................................... 37

Using WPI Data Acquisition Systems in Cardiovascular Applications ........................................................................................................38

CELS Temperature Transducer ..............................................................................................................................................................................40

LUME Light Transducer ..........................................................................................................................................................................................40

PNEU Pressure Transducer ....................................................................................................................................................................................40

DSPL Displacement Transducer ...........................................................................................................................................................................40

FORT Force Transducers ......................................................................................................................................................................................... 41

Dual Range Force Transducer ................................................................................................................................................................................ 41

25g Force Transducer .............................................................................................................................................................................................. 41

FOMS Fiber Optic Measurement System for blood pressure .......................................................................................................................42

ISO-DAM8A Modular DC Ampliers ....................................................................................................................................................................44

Bridge8 Modular Transducer Amplifiers ............................................................................................................................................................44

DBA8000 Digital Bridge Amplifier for Pharmaceutical Drug Screening ..................................................................................................46

Myobath II Multi-Channel Tissue Bath ..............................................................................................................................................................48

Haake DC10 Circulating Bath ................................................................................................................................................................................53

EpithelialEVC4000 Multi-Channel Voltage/Current Clamp ............................................................................................................................................54

Acquiring Data from the EVC4000 ......................................................................................................................................................................55

Ussing System ............................................................................................................................................................................................................56

DVC-1000 Two-Channel Voltage/Current Clamp ............................................................................................................................................58

EVOM Epithelial Voltohmmeter ............................................................................................................................................................................59

EVOMX Epithelial Voltohmmeter with BNC output ........................................................................................................................................60

STX100 Electrodes for 24-well HTS plates .......................................................................................................................................................60

CaliCell™ culture cup with synthetic membrane for testing electrodes and chambers .......................................................................60

Multi-96 resistance measurement probe for 96-well PAMPA plates ........................................................................................................ 61

Endohm™ chambers for TEER measurement of cell cultures in individual cups .................................................................................. 61

REMS Automated TEER Measuring System ......................................................................................................................................................62

REMS-KIT — add WPI’s TEER electrode to your own robot sampler .......................................................................................................63

MiscellaneousMicroprobe Thermometers .....................................................................................................................................................................................64

ATC1000 Animal Temperature Controller ..........................................................................................................................................................66

MPS-2 Multichannel Perfusion System ..............................................................................................................................................................67

Vibroslice manual and motorized microtomes ...............................................................................................................................................68

IntegraSlice™ Oscillating Blade Microtome .....................................................................................................................................................69

Peltier Temperature Controllers for Vibroslice ...................................................................................................................................................69

Lab-Trax™ 4- and 8-channel data acquisition hardware .............................................................................................................................. 70

WPI-118 High Performance Digital Data Recorder ........................................................................................................................................ 71

Data-Trax™ software for data acquisition and analysis ................................................................................................................................72

Data Analysis with Data-Trax™ ........................................................................................................................................................................... 74

FluoroDish™ glass bottom dishes ....................................................................................................................................................................... 76

Cover Slips .................................................................................................................................................................................................................. 77

ABM Audible Baseline Monitor ............................................................................................................................................................................ 77

LPF30 Low-Pass Filter ............................................................................................................................................................................................. 77

900A Micropressure System .................................................................................................................................................................................. 78


World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: [email protected] • Internet: www.wpiinc.com

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Transbridge (TBM4M) is a four-channel analog transducer manifold, specifically designed to amplify output voltage signals from pressure, force, displacement, and temperature transducers as well as a wide variety of other signal sources. Analog output signals available from each channel can be recorded on magnetic tape, displayed on an oscilloscope or applied to the input of a multichannel data acquisition for digital signal processing in a computer.

Each channel contains a regulated 10-volt power supply (+5 and -5 volts with respect to signal ground) to provide DC power to transducers, and a precision differential amplifier with selectable voltage amplification and variable position adjustment control.

Transducers can be connected to Transbridge via any of the 8-pin connectors on the front panel. Four spare 8-pin DIN plugs are provided with each instrument to allow you to rewire cables of other manufacturers’ transducers and connect them to Transbridge. Each Transbridge channel may be used in either Full Bridge or the Half Bridge mode independently. For transducer types other than resistive bridges, such as active transistor circuits, magnetic, photocell or piezoelectric devices, the instrument’s differential amplifiers may still be used effectively for signal amplification in differential (full bridge) and single-ended (half bridge) modes. See WPI’s complete line of TRANSDUCERS for physiological measurements.

4-Channel Transducer Amplifier

Transducers not included. See WPI’s complete line of transducers for physiological measurement.

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TRANSBRIDGE SPECIFICATIONSCHANNELS 4

VOLTAgE AMPLIFICATION x1, x10, x100, x1000

VOLTAgE OFFSET ADjUSTMENT > ± 50 mV

NOISE .4uV p-p (0.1 to 10Hz, g=100)

LINEARITy +/-.001% of FSR g=1; +/-.01% of FSR, g-=1000

OUTPUT VOLTAgE SWINg ± 10 V

MAxIMUM OUTPUT CURRENT 2 mA

INPUT IMPEDANCE, EACH INPUT 1010 ohms

TRANSDUCER ExCITATION 10 V DC (±5 V) approx.

BANDWIDTH, SMALL SIgNAL 1 MHz (x1), 80 KHz (x10), 10 KHz (x100), 1.0 KHz (x1000)

DIMENSIONS 8.5 x 5.12 x 10 in. (21.6 x 13 x 25.44 cm)

SHIPPINg WEIgHT 11 lb (5 kg)

FORT100

PNEU DSPL



TBM4M Transbridge Transducer Amplifier


OPTIONAL ACCESSORIES13024 Single Rack Mount Kit13025 Dual Rack Mount Kit500184 BNC-to-BNC cable, 10 ft3161 8-pin DIN plug3718 Package of 4, 8-pin DIN (startup kit)

See Transducers, pages 49, 52, 53

TRANSDUCERSCELS Temperature Transducer PNEU01 Pressure Sensor, 1 psigPNEU05 Pressure Sensor, 5 psigPNEU15 Pressure Sensor, 15 psigDSPL Displacement TransducerLUME PhotocellFORT10-100 Force Transducer, Dual Range (10 g and 100 g) FORT25 Force Transducer (25 g) FORT100 Force Transducer (100 g) FORT250 Force Transducer (250 g) FORT1000 Force Transducer (1000 g) FORT5000 Force Transducer (5000 g) 3491 Probe Extension Cable

Caution: Extension cable may diminish signal-to-noise ratio.



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BLPR2 can be used for the direct arterial and venous pressure measurement in animal blood vessels. Supplied sterile, BLPR2 is accurate, linear and stable with temperature. May be sterilized cold with Cidex or a similar bactericide.

BLPR2 is equipped with a twelve-foot cable and connector compatible with WPI’s four-channel signal conditioning unit, TBM4M Transbridge, and the single-channel BP1 blood pressure monitor. Cable has moisture-resistant locking connector. A continuous, uniform lumen eliminates places for bubbles to form and lodge. The clear fluid path is easy to inspect. Easy to mount — slotted transducer body accepts Velcro strap.

To facilitate setup and operation, a four-way stopcock that allows easy filling, flushing, and zeroing of the transducer is included. Typically, the stopcock is located between the transducer and the animal catheter where it can be used to quickly zero, flush, or de-bubble the transducer.

Blood Pressure Monitor and Transducer● Monitors pressure, force, temperature● Monitors animal arterial or venous blood pressure● Displays systolic, diastolic or average blood pressure

BP1 accepts WPI’s BLPR blood pressure transducer (below) as well as other blood pressure transducers.

An audio monitor provides a signal with variable pitch and amplitude, allowing you to hear changes in blood pressure. Digital LCD display provides average or peak signal values from 0 to 1999 mV. With an optional pressure gauge (not provided — see PM015D/R, page 239), the user may calibrate the display to read mm Hg. Recorder output connector allows direct connection to a pen recorder, oscilloscope or computer via a data acquisition system.

BP1 SPECIFICATIONSAMPLIFICATION x1, x10, x100, variable (x5 to x1000)

BANDWIDTH, SMALL SIgNAL (-3 dB) 300 kHz (x1)

30 kHz (x10)

3 kHz (x100)

0.3 kHz (x1000)

OUTPUT VOLTAgE SWINg ± 5 volts

MAxIMUM OUTPUT CURRENT 2 mA

INPUT IMPEDANCE, EACH INPUT 100 kΩ || 0.01 µF

TRANSDUCER APPLIED VOLTS 10 V nominal, varies with load. 25 mA,

maximum

DIgITAL PANEL METER 0 to 1999 mV (calibrated in mm of Hg

pressure when supplied with pressure

gauge). Meter reads average value or

± peak (1 to 20 Hz).

POWER 95-135 V or 220-240 V, 50/60 Hz

DIMENSIONS 8.5 x 5.12 x 10 in. (21.6 x 13 x 25.44 cm)

SHIPPINg WEIgHT 11 lb (5 kg)

BLPR2 SPECIFICATIONSWORKINg PRESSURE -50 to + 300 mm HgOVERPRESSURE -400 to +4000 mm HgExCITATION VOLTAgE 1-10 VDC or RMS to 5 kHzINPUT IMPEDANCE 350 Ω ±10%OUTPUT IMPEDANCE 300 Ω ±10%ISOLATION < 5 µA leakage at 120 VACSENSITIVITy 5 µV-/-V-/-mm HgDyNAMIC RESPONSE 100 HzEIgHT HOUR DRIFT 1 mm Hg after 5 minute warm-upMAxIMUM ERROR Total combined effects of Sensitivity, Linearity, Hysteresis (at 25°C and 5 µV/V/mm Hg) do not exceed ±2% or 1 mm Hg, whichever is greater.ENVIRONMENTAL Operating Temp. Range +15°C to +40°C Storage Temp. Range -30°C to +60°C Light Sensitivity Not affected by light under 3000 ft/candles (32,000 lux)SHIPPINg WEIgHT 1 lb

BLOOD PRESSURE TRANSDUCER

Stopcock #14036 included with BLPR2



BP1 Pressure Monitor (transducer & cable not included)

Specify line voltageACCESSORIESBLPR2 NEW Blood Pressure Transducer & CableBPCABLE2 NEW Cable (12 ft) with DIN connector for BLPR2503067 BLPR2 Transducer without cable13024 Single Rack Mount Kit13025 Dual Rack Mount Kit3491 Extension Cable, 5 ft500184 BNC-to-BNC Cable, 10 ft14036-15 4-Way Luer Stopcock, Blue Tint (package of 15) KZ1101 Micro Cannula, 3 inch

Note: BLPR2 is intended for animal research only and may not be used for human blood pressure measurement.

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Cardiac Hemodynamics

Hemodynamics is the study of the dynamic behavior of blood as it flows around the heart, the lungs and the body. Pressure is generated in various parts of the heart as a result of this movement. These pressures can be measured and monitored through catheters whose tips are placed in

the atria, pulmonary artery, or systemic arteries.Hemo-dynamic studies require the measurement of

pressure, flow, and electrical signals from the heart, and electrical signals from nerves, such as the vagus. WPI has everything you need for research studies in hemody-namics, and associated studies in cardiac electrophysiol-ogy and cardiopulmonary function.

Simultaneously record and Automark LDEVP (Channel 1) and systolic and dichrodic notch values (Channel 2) from a blood pressure curve.

Using WPI Data Acquisition Systems in Cardiovascular Applications

HardwareThe Lab-Trax 4/24T is the perfect recorder for cardiovascular applications. The T Series has built-in transducer amplifiers so blood pressure transducers or other transducers plug directly in. Each channel has its own independent stimulator output so complex waveforms can be generated as well as simple things like vagal

stimulation or extra stimulus pacing of cardiac preparations. In addition, 8 programmable digital

I/O lines can control pumps or drug delivery in the laboratory environment. The Lab-Trax series

connects to your computer via the USB port so installation is truly plug and play.

SoftwareOnline Analysis

Data-Trax can calculate rate, systolic, diastolic and mean pressures, as well as dp/dt and integral from any raw data input in real time. All of the same functions can be applied offline, too.

A powerful baseline-tracking algorithm ensures that even if your signal moves or changes shape, the calculated rate or systolic/diastolic/-mean pressure is faithfully reported.

Offline Analysis

All of the online functions can be applied offline. In addition, a series of functions applied from the Analysis window can be used to reduce raw data. Functions operate on a selection of data and return a single value, which can be written to the Data-Trax Journal. Currently supported functions

For complete description, see page 70.





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include: mean, integral, area, dp/dt, mean dp/dt, mean systolic, mean diastolic, and mean rate. Analysis window functions used in conjunction with the Data-Trax Find command, can automatically analyze the entire record.

Input/Output

The built-in stimulator allows delivery of a single, continuous, or train of pulses. Pulse durations, frequencies and amplitudes can be changed as the protocol is being delivered. The software also offers full control of digital 1/0 lines that can be used to count events, or control devices in the lab environment.

Display

Data-Trax allows you to easily convert voltage to real units, such as mmHg or percent for pressure or flow measurements, respectively. The display allows you to toggle between a preferred Y-axis scaling (between 40 and 140 mmHg, for example) and the “best view” as determined by the AutoScale feature. Alternately, you can zoom-in or zoom-out by factors of two with a single mouse click. In a similar fashion, X-axis scaling can be expanded or compressed by factors of two with a single click.

Real Time Annotations

While recording, you can mark your data with text messages. These marks are searchable and appear in the field of data to which they apply. Marks are time locked to the record and can be used to “Go To” any marked region of the data.

Data Reduction

Data can be analyzed and reduced in the Analysis window. Here, selections of data can be averaged and written to a report. For example, the software can be instructed to break the record up into five-minute pieces, determine the average systolic or average heart rate for the data in each segment, and write those values to a report.

XY Window

Any channel can be displayed in XY format. Data-Trax supports area measurements and segment slopes from such plots. And, of course, you can save the plots as pictures or text.

Export

All recorded data can be exported to a MatLab, DADiSP, Excel or text file or as a picture (.png), making reports or posters much easier to create. Of course, you can always print your data from any window at any scale.

XY Window

Capture data and post to the Marks Window to preview prior to export.

Easily export data to Excel or other analytical program for further analysis.

Using WPI Data Acquisition Systems in Cardiovascular Applications

For more information about Data-Trax, see pages 72-75.



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Transducers for Physiological MeasurementThese transducers are designed for general use in data acquisition and recording instruments and systems. They are supplied with 5-ft cables terminating in an 8-pin DIN connector which readily plugs into Transbridge, a 4-channel analog signal conditioner and Bridge8 . Transducers can be easily converted with adapter cables and plugs to work with most standard recording systems and polygraphic instruments.

PNEU01, PNEU05, PNEU15 SPECIFICATIONSWORKINg PRESSURE PNEU01 0-1 psi, differential (52 mm Hg) PNEU05 0-5 psi, differential (260 mm Hg) PNEU15 0-15 psi, differential (780 mm Hg)MAxIMUM PRESSURE 3x Full Scale PressureTRANSDUCER SENSITIVITy PNEU01: 18 mV, Full Scale (±10% at 10 V) PNEU05: 60 mV, Full Scale (±10% at 10 V) PNEU15: 90 mV, Full Scale (±10% at 10 V)SUPPLy VOLTAgE 10 V (±5 V)INPUT & OUTPUT RESISTANCE 4 kΩ, nominalLINEARITy ERROR 0.5% maximum

DSPL SPECIFICATIONSDISPLACEMENT 24 mm (±12 mm)OUTPUT VOLTAgE RANgE 16 mV /mm (± 10% with 10 V DC applied)OUTPUT RESISTANCE 10 kΩ, differentialNOISE 0.5 mV p/p (10 kHz bw), typicalDRIFT 0.05% Full Scale / change in °CExCITATION VOLTAgE 7 V DC minimum to 12 V DC maximumCURRENT DRAIN 10 mA typical, 20 mA, maximumLINEARITy 2 % STyLUS WEIgHT 2.5 gramsSTyLUS STATIC FRICTION 1.0 gram

CELS SPECIFICATIONSTEMPERATURE RANgE 10 to +100 degrees Celsius

OUTPUT 10 mV per degree C ± 2%

OUTPUT RESISTANCE 270 ΩPROBE 5 mm diam x 100 mm length, stainless steel, epoxy tip

SUPPLy VOLTAgES ±25 V maximum

SUPPLy CURRENT 150 µA maximum

LUME SPECIFICATIONSPOSITIVE SUPPLy VOLTAgE +50 V, maximum

NEgATIVE SUPPLy VOLTAgE -20 V, maximum

LIgHT VOLTAgE 50 mV minimum (per 5 mW/cm2 with 2870°K source)

DARK OUTPUT VOLTAgE 100 µV max. (+ 5 V supply)

ANgULAR FIELD OF VIEW ± 12 degrees off axis

SPECTRAL RANgE 400 - 1200 nm (peak at 860 nm)

PROBE 5 mm diam x 100 mm, stainless steel

LUMEDSPL

PNEU

CELS



DISPLACEMENTDSPL smoothly converts mechanical displacement into a proportional voltage with

virtually infinite resolution and essentially zero frictional drag. Based on the Hall effect, which uses a magnetic sensor.

DSPL Displacement Transducer3491 Probe Extension Cable


PRESSUREPNEU transducers are accurate differential pressure measuring devices for noncorrosive gases and gaseous fluids. Linear and repeatable, with little temperature drift, they convert hydrostatic pressure levels to stable and proportional voltage signals.

PNEU01 Pressure Sensor, 1 psigPNEU05 Pressure Sensor, 5 psigPNEU15 Pressure Sensor, 15 psig3491 Probe Extension Cable


TEMPERATUREBased on an integrated-circuit temperature sensor, CELS moni-tors temperature at the probe tip and produces 10 millivolts per degree Celsius when powered by an excitation source of 10 volts (±5 V). Stainless steel probe with epoxy tip.

CELS Temperature Transducer3491 Probe Extension Cable


LIGHTWhen powered by +2 volts or more, LUME provides an output voltage signal proportional to inci-dent light over the visible to near infra red spectrum. Containing a miniature silicon photodiode, the slender stainless steel probe can be easily mounted to do a wide variety of useful jobs, such as monitoring transmitted, reflected or interrupted light beams. Optic fibers up to 2 mm diameter may be inserted into the tip.

LUME Photocell3491 Probe Extension Cable




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FORCE TRANSDUCERSThese rigid-lever force transducers transform applied force into propor-tional voltage. Using balanced strain gauges, FORT transducers produce linear output voltage vs. applied force input with very little deflection.

To use, clamp the handle of the FORT transducer in a horizontal position and apply the forces to be measured to a rivet or hook mounted in the hole at the end of the flat sensing leaf.

FORT 100

FORT 250

FORT 1000

FORT 5000

25g Force TransducerThis 25-gram force transducer is a reliable tool for high precision force measurement. Using balanced semiconductor strain gauges, FORT25 produces linear output voltage vs. applied force input with very little deflection. The rigid lever force transducer transforms the applied force into a proportional voltage. It is a temperature-compensated full-bridge configuration with four high sensitivity semiconductor strain gauges. FORT25 has a broad dynamic measuring range and a very high sensitivity.

To use, clamp the handle of the FORT25 transducer in a horizontal position and apply the forces to be measured to a rivet or hook mounted in the hole at the end of the flat sensing leaf.

FORT25 SpecificationsFORCE RANgE, FULL SCALE 0-25 grams

OUTPUT SENSITIVITy 300 µV/volt/gm, nominal

INPUT & OUTPUT RESISTANCE 1500 ΩRESOLUTION < 2mg

RESONANT FREqUENCy 450 Hz

LINEARITy ERROR 0.5%

MAxIMUM OPERATINg VOLTAgE 10 V DC (-5V ~ +5V or 0 ~ 10V)

MAxIMUM APPLIED FORCE 3× rated full scale force

DIMENSIONS 40mm × 22mm × 19mm

WEIgHT 100 gram

Dual Range Force TransducerThe FORT10-100 is a high sensitivity dual-range research grade force transducer designed to measure forces in the 0.005 to 10 gram and 0 to 100 gram ranges. The FORT10-100 exhibits excellent isometric properties. In addition, its unique optical technology makes it resistant to drift and offers intrinsically low noise characteristics. The FORT10-100’s on-board amplifiers reduce gain and adjustment requirements on recording amplifiers to which it may be connected.

The transducer is highly resistant to mechanical damage; its body is machined from aluminum and mounts to a standard ring stand or, with the handle removed, clamp mounts. The cable that connects the FORT10-100 to the recording amplifier is detachable, allowing for multiple cables to be configured for the user’s choice of amplifiers. FORT10-100 requires a transducer conditioning amplifier such as the Bridge8 or TBM4M.

FORT SPECIFICATIONSFORT10-100 FORT100 FORT250 FORT1000 FORT5000

FORCE RANGES, FULL SCALE 10 grams or 100 grams 100 grams 250 grams 1000 grams 5000 gramsOUTPUT SENSITIVITY (± 10%) 75-100 µV/V/g, 7.5-10 µV/V/g 7 µV/V/g 3 µV/V/g 0.84 µV/V/g 0.38 µV/V/gINPUT & OUTPUT RESISTANCE N/A 350 Ω 350 Ω 350 Ω 350 ΩRESOLUTION 0.03% of full scale force 0.01% of full scale force 0.01% of full scale force 0.01% of full scale force 0.1% of full scale forceRESONANT FREQUENCY — 300 Hz 300 Hz 300 Hz 60 HzLINEARITY ERROR < 0.1% of full scale Less than 0.1% of full scale Less than 0.1% of full scale Less than 0.1% of full scale Less than 0.1% of full scaleMAX. OPERATING VOLTAGE ± 5V DC 10 V AC or DC 10 V AC or DC 10 V AC or DC 10 V AC or DCMAXIMUM APPLIED FORCE 1× rated full scale force 3× rated full scale force 3× rated full scale force 3× rated full scale force 3× rated full scale force

DRIFT<30 mg/hr, thermally compensated

thermally compensated thermally compensated thermally compensated thermally compensated

DIMENSIONS1.125” x 3” x 0.6”Handle 2.54” x 0.38” diam

0.3 inch diam × 4 in.(7.6 mm diam × 10.2 mm)




WEIGHT (excluding cable) ____________ 0.3 oz (8 g) 0.3 oz (8 g) 0.3 oz (8 g) 0.3 oz (8 g)



FORT100 Force Transducer (100 g) FORT250 Force Transducer (250 g) FORT1000 Force Transducer (1000 g) FORT5000 Force Transducer (5000 g)

FORT25 Force Transducer (25g)

FORT10-100 Force Transducer (10g and 100g)

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cavity of the sensor tip via a multimode fiber with an outer diameter of 170 µm (0.5 F). The measurement tip is insensitive to any pulling or manipulation of the fiber. All the materials used in the sensor tip are biocompatible and are anti-thrombogenic. As such, the sensor can be positioned even in regions where the blood flow rate is high, e.g., the abdominal aorta.

Fiber Optic Measurement SystemIn order to obtain blood pressure measure-ment, the FOBPS must be connected to the Fiber Optic Measurement System (FOMS), which is dedicated for use with the FOBPS and offers 1000 Hz bandwidth (via analog ouput). This bandwidth is fast enough to capture any details of pressure tracing (e.g., dicrotic notch), especially for small animals such as rats or mice. The FOBPS design incorporates a memory chip that stores its unique calibration parameters. This elegant design makes the system very convenient to use. Once the FOBPS is connected to the FOMS, the meter captures the calibration parameters and adjusts automatically to deliver precise pressure measurement data. The user can easily establish an arbitrary zero reference point (i.e., for 0 mm Hg) within the pressure operating range by pressing a single button. These two steps encompass the entire calibration process for the measurement system.

FOBPS is a unique family of fiber optic pressure sensors incorporating the design advantages of both internally placed catheter-tip sensors and more conventional invasive methods in which catheter fluid is coupled directly to an external transducer (WPI #BLPR2). The tips of the FOBPS sensors range from 1.3 F (430 µm) to 2.8 F (914 µm) so that they can be implanted directly at the measurement site (e.g., intra cardiac, blood vessel) for different sized animals. This sensor is not subject to the physical artifacts typically associated with similar invasive blood pressure measurement devices which utilize

Fiber Optic Measurement System

Immune to EMI/RFI and MRI, MW/RF compatible ● Small sensor size: diameter = 590 µm, 1.8FIntrinsically electrically isolated ● Fast, automated calibration of sensors

1000 Hz bandwidth ● BiocompatibleSensors are re-usable ● High accuracy and fidelity

an external transducer. Furthermore, the use of noise-immune fiber optic communication eliminates problems associated with electrically energized transducers when recording data in strong electromagnetic or RF fields, such as those generated by electronic scalpels or MRI systems. WPI also offers fiber optic blood pressure sensors with extra long 60 ft (18 m) fiber connection length for MRI applications. This allows the FOMS instrument to be located an adequate distance from the MRI field coil to ensure the lowest possible noise. No signal is lost across the length of the fiber. Like the entire family of FOBPS sensors, the FOBPS MRI

sensors are MRI translucent and have complete immunity to EMI, RF, ESD, and MRI field potentials. These new sensors are available in all standard sizes (FOBPS-14LG: 1.4 F; FOBPS-18LG: 1.8F; and FOBPS-28LG: 2.8F).

Principles of OperationA small cavity is embedded in the sensor tip. Blood pressure is measured by observing the changes in length of the cavity by way of an optical signal conditioner using a measurement scheme based on white light interferometry. Sensing light is transmitted to and reflected back from the detecting diaphragm and

FOMS SPECIFICATIONSNumber of Channels 1

Measurement Range -50 to 300 mmHg

Accuracy ±2 mmHg or ±3% at reading, whichever is greater

Display Resolution 1 mm Hg

Output Voltage Range -0.5 to 3.0 VDC, 10 mV/mm Hg

Frequency Response DC to 1000 Hz

Temperature 15 to 35°C

Display 4-digit LED display

Power Requirement 110/220 VAC, 50/60 Hz

Dimensions 10 x 10 x 2.75 in

Weight 4.5 lb. 2.25 kg





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ApplicationsIn vivo pressure monitoring:

● Blood Pressure Intra-uterine Intra-cranial Intra-ocular Intra-peritoneal

● Research Applications

● Product Development

with biologically compatible pressure sensors

A recording was made with the FOBPS-18 sensor located in the aorta of a rat. The resolution of the sensor is demonstrated by the articulation of the dichrotic notch.

A recording was made with the FOBPS sensor located within the left ventricle of a rat. The relative smoothness of the wave-form peaks indicate an absence of “whipping effect”, which can occur with sensors that are too large to avoid contact with the ventricular walls during contraction.

Blood pressure tracing in aorta of a rat

50

60

70

80

90

100

110

120

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

Pre

ssu

re (m

mH

g)

Time (Seconds)

Typical pressure tracing in left ventricle of a rat

0

20

40

60

80

100

120

140

0 0.5 1 1.5 2

Time (Seconds)

Pre

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mH

g)

FOBPS SPECIFICATIONS FOBPS-13 FOBPS-13LG FOBPS-18 FOBPS-18LG FOBPS-28 FOBPS-28LG

Sensor 1.3 F (430 µm) 1.3 F (430 µm) 1.8 F (590 µm) 1.8 F (590 µm) 2.8 F (914 µm) 2.8 F (914 µm)

Catheter - - 0.5 F (170 µm) 0.5 F (170 µm) 2.8 F (914 µm) 2.8 F (914 µm)

Effective length 2”, 5cm 2”, 5cm 12”, 30 cm 12”, 30 cm 12”, 30 cm 12”, 30 cm

Total Catheter length 6’, 180 cm 60’, 18 m 6’, 180 cm 60’, 18 m 6’, 180 cm 60’, 18 m

Catheter material Polyimide Polyimide Polyimide Polyimide Nylon Nylon

Rat yes yes yes yes yes yes

Mouse yes yes yes yes No No

Repairable No No No No No No

Reusable yes yes yes yes yes yes

1.3 F430 µm

50 mm

5 mm1.8 F (590 µm)

0.5 F (170 µm)

FOBPS-13

FOBPS-18

1.8 F590 µm

5 mm

Optical FiberDiam: 0.5 F (170 µm)

FOBPS-28

2.8 F914 µm

30 cm

Optical Fiber



FOMS Fiber Optic Measurement System FOMS-13 FOMS and FOBPS-13 Sensor FOMS-18 FOMS and FOBPS-18 Sensor FOBPS-13 Fiber Optic Blood Pressure Sensor, 1.3 F, length 6 ft (180 cm) FOBPS-13LG Fiber Optic Blood Pressure Sensor, 1.3 F, length 60 ft (18 m) FOBPS-18 Fiber Optic Blood Pressure Sensor, 1.8 F, length 6 ft (180 cm) FOBPS-18LG Fiber Optic Blood Pressure Sensor, 1.8 F, length 60 ft (18 m) FOBPS-28 Fiber Optic Blood Pressure Sensor, 2.8 F, length 6 ft (180 cm) FOBPS-28LG Fiber Optic Blood Pressure Sensor, 2.8 F, length 60 ft (18 m) 2851 Cable, Standard BNC, 6 ft (1.8 m)

See Lab-Trax data acquisition system, page 82.

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Iso-DAM8A Modules Isolated Low Noise Bio-Amplifier

Optional Recording probe

#74040 plugs into theIso-DAM8A input jacks.

The ISO-DAM8A is a compact modular standard rack-mountable DC amplifier system. Each channel is electrically isolated from the others and from ground. No current can flow from the input terminals and electrodes, thus, the instrument is intrinsically safe and cannot cause any electrical stimulus or shock to the preparation, in addition ground loop noise is minimized. Systems can be purchased with one, two, three or up to eight preamplifier modules or mixed with Bridge8 transducer amplifier modules (see next page). The user can then select an appropriate low pass filter setting, gain and offset on the channel amplifier panel. A notch filter has been added to reduce line frequency interference. An optional headstage preamplifier (10x gain) allows low noise extracellular (DC) recording with Iso-DAM8A and adds greater signal bandwidth than a shielded cable of the same length. The Iso-DAM8A amplifier and headstage configuration is optimally suited for use with our metal microelectrodes and can be easily configured for many applications. Each amplifier channel has a coaxial (BNC) connector located on the rear panel.

ISO-DAM8A SPECIFICATIONS

EACH CHANNEL

INPUT IMPEDANCE > 1012 Ohms

INPUT LEAKAGE CURRENT 10 pA (typical)

INPUT DC OFFSET ± 100 mV

GAIN ×10, ×100, ×1000, ×10,000

COMMON MODE REJECTION > 100 dB @ 50/60 Hz

EQUIVALENT NOISE SIGNAL INPUT < 0.36 µV rms (1.8 µV p-p) 0.1-10 Hz, Gain>10 < 1 µV rms (5 µV P-P) 0.1-10 kHz

BANDWIDTH FILTER SETTINGS High Filter (Low Pass) (kHz) 0.1, 0.5, 1, 3, 10 Low Filter (High Pass) (Hz) 0.1, 1, 10, 300 Notch Filter (Hz) 50, 60

OUTPUT VOLTAGE SWING ± 7.5 Volts

OUTPUT RESISTANCE 220 Ohms

SYSTEM

ENCLOSURE DIMENSIONS 7 × 17 × 9.2 in. (18 × 43 × 23 cm)

SHIPPINg WEIgHT 10 to 21 lb (4.5 to 9.5 kg)

ISDB System lets you combine modules in the same chassis



SYSTEMS Specify any combination of ISO-DAM8A or BRIDGE8 module (s)ISDB-1 Bio-amplifier System (1 channel) Specify ISO-DAM8A or BRIDGE8 module ISDB-2 Bio-amplifier System (2 channels) Specify ISO-DAM8A or BRIDGE8 modulesISDB-3 Bio-amplifier System (3 channels) Specify ISO-DAM8A or BRIDGE8 modules ISDB-4 Bio-amplifier System (4 channels) Specify ISO-DAM8A or BRIDGE8 modules ISDB-5 Bio-amplifier System (5 channels) Specify ISO-DAM8A or BRIDGE8 modules ISDB-6 Bio-amplifier System (6 channels) Specify ISO-DAM8A or BRIDGE8 modulesISDB-7 Bio-amplifier System (7 channels) Specify ISO-DAM8A or BRIDGE8 modules ISDB-8 Bio-amplifier System (8 channels) Specify ISO-DAM8A or BRIDGE8 modules

Each configuration above includes enclosure, power supply, and blank panels for empty slots.

Included with each ISO-DAM8A bioamplifier:8-pin DIN connector with shielded, unterminated 5-ft cable.




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Bridge8 ModulesLow Noise Transducer Amplifier

Bridge8 is a modular, rack-mountable amplifier system based on a similar architecture to the popular ISO-DAM8A amplifier system. Bridge8 is specifically designed to be used as a signal conditioning amplifier with strain gauges and other powered transducers. Bridge8 includes differential amplifiers featuring high input impedance, high common mode rejection and low current leakage input terminals for low noise operation. The Bridge8 also features a half bridge switch and channel offset A wide variety of WPI transducers are available for force, temperature, pressure and light measurements. The Bridge8 amplifier is a clear choice where convenience and quality are required. Any combination of ISO-DAM8 or Bridge8 modules may be combined in the same enclosure. The ISO-DAM8A module is also available in this rack configuration.

BRIDGE8 SPECIFICATIONSINPUT IMPEDANCE > 1012 Ohms

AMPLIFICATION 1, 10, 50, 100, 500, 1000 & Adjustable

INPUT LEAKAGE CURRENT 0.1 pA at 25 °C

VOLTAGE OFFSET ADJUSTMENT ±50 mV (low); ±100 mV (high)

AMPLIFIER OUTPUT VOLTAGE ±4.4 volts (10 mA, max.)

EXCITATION VOLTAGE 10 volts (±5.0 V) 100 mA, max.

EQUIVALENT NOISE SIGNAL INPUT < 0.4 µV RMS (2 µV p-p) 0.1-10 Hz Gain >10 < 3 µV RMS (15 µV p-p) 0.1-100 Hz Gain >10

LOW PASS FILTER BAND (KHz) 0.03, 0.1, 0.3, 1, 5, 10, "Wide Band" R-C Butterworth 6 dB /octave

SYSTEM ENCLOSURE DIMENSIONS 7 x 17 x 9.2 in.

SHIPPING WEIGHT 10 to 21 lb ( 4.5 to 9.5 kg )

Yes, Bridge8 and Iso-DAM8A modules can be

mixed in the same chassis!

ISDB System lets you combine modules in the same chassis



OPTIONAL ACCESSORIESFORT25 Force Transducer 25 gFORT100 Force Transducer 100 gFORT250 Force Transducer 250 gFORT1000 Force Transducer 1000 g47610 Force Transducer 100 g Myobath onlyBLPR Blood Pressure Transducer & 12‘ (3.6m) CablePNEU01 Pressure Sensor, 1 PSIg (6.8 Kpa) PNEU05 Pressure Sensor, 5 PSIg (34.4 Kpa) PNEU15 Pressure Sensor, 15 PSIg (103.4 Kpa) DSPL Displacement Transducer (± 12mm) CELS Temperature Transducer –10 to 100 °CLUME Photocell (50mV / 5mW/cm2) 74020 Iso-DAM8A Single Channel Module74030 ISDB chassis and power supply74040 Iso-DAM8A Active Headstage (separate) 74050 ISDB Blank panelsBRIDGE8 Bridge8 Transducer Amplifier Module2933 ISDB Rack Mount Kit, 51/4-in. High500184 BNC to BNC 10 foot cable3161 8-pin DIN plug3718 Package of 4, 8-pin DIN (startup kit) 3491 Extension Cable (DIN male, DIN female), 5 ft (1.5m)

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The DBA8000 is a compact state-of-the-art Digital Bridge Amplifier. It is built to the latest standards in digital and analog technology. WPI’s Digital Bridge Amplifier (DBA8000) has 8 identical channels that can be configured and calibrated independently and used as a Full Bridge Amplifier, Half Bridge Amplifier or as a Differential Amplifier (DC coupled). In any of these modes, analog input signals are converted in real-time into digital values (24-bit resolution) and processed by a Digital Signal Processor (DSP) microcontroller. Real-time digital signals are then converted into analog signals that are available from the BNC output sockets. This same digital data are also made available at the Ethernet port or displayed on the LCD in a resolution up to five significant figures.

In the bridge amplifier mode, eight digital signal synthesizers generate the excitation amplitudes (with the desired phase and frequency) for the bridges. All parameter settings can be stored in up to 10 different parameter memories to ensure quick installation and start-up. The DBA8000 is fully menu driven using the touch screen LCD that displays menus and enables the operator to select or enter data. A password protected hierarchical structure limits the privileges for users and owners; the administrator always has full control over all parameters of the DBA8000. The Digital Bridge Amplifier’s programmed software parameters and, or, memories can be exported to (or imported from) an FTP server for easy updates. The DBA8000 is fully compatible with most brands of biomedical transducers and is pre-configured for WPI’s popular FORT force transducers. What makes this amplifier so unique is that measurements from experimental subjects can be displayed in the standard units (such as mmHg, mmO2, mV, KPa) or any other units that can be programmed with defined parameters. Indeed the DBA8000 is the new workhorse of Bridge Amplifiers!

DBA8000 for pharmaceutical drug screening

● The World’s Most Advanced Digital Bridge Amplifier

● Eight Analog Output Channels

● Eight Analog Inputs With Independent Excitation Voltages

● LCD With Touch Screen Input And Scope Screen Dump

● Serial Port and/or Ethernet Telnet FTP Communications

● Direct Digital Output Via TCP/IP (Compatible With Generic Acquisition Software)

● Parameter Menus Store Different Experimental Setups

● Easy Transducer Calibration and Testing

● Selectable and Programmable Output Gains

● GLP /GCP Compliant With Password Protection

Calibration Settings Screen

The Calibration Settings Screen is used to assign minimum and maxi-mum display values to acquired reference measurements. The acquisition time over which a calibration value will be averaged can be adjusted to enable correct calibration with minimum noise. Use the “Min. & Max.” and “Acquisition” functions keys to copy the calibrated values and acqui-sition time from channel one to all channels. This time saving feature eliminates the tedious process of calibrating each channel independently when using the same transducer in multiple channels. Note that the units of measurement are shown for each channel.

Includes 4

transducers of

your choice!Includes 4

transducers of

your choice!



DBA8000 Digital Bridge Amplifier Includes 4 transducers — specify when ordering

15657 grass Transducer Cable

See transducers, pages 52, 53.



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DBA8000 for pharmaceutical drug screening

DBA8000 SPECIFICATIONSNUMBER OF CHANNELS 8 independent channels.

BRIDGE INPUT MODES Full bridge, half bridge, differential amplifier

BRIDGE EXCITATION Fully differential, 0 to 15 V DC or 0 to 10Vrms, 500 to 15,000 Hz (sine wave)

BRIDGE DIGITAL OUTPUT Via Serial or TCP/IP socket over Ethernet at up to 1000 samples/sec maximum

BANDWIDTH 1000 Hz

FILTERING: LOW PASS 4 Hz to 1000 Hz

FILTERING: 50/60 Hz Variable to 120 dB depending on band pass (120 dB @50/60 Hz / 6 Hz pass)

DIGITAL REMOTE CONTROL 24 digital I/O lines

ABSOLUTE MAX. INPUT VOLTAGE + or – 12 V DC

ABSOLUTE MAX. INPUT CURRENT + or – 50 mA

SAMPLE FREQUENCY 240 kHz before the digital LPF (Low Pass Filter), 125 to 3750 Hz after the LPF

INPUT IMPEDANCE 110K ohm (+/-10V) 1 G ohm (20 mV to 1280 mV ranges)

MAX. OUTPUT VOLTAGE -10 to +10 V @ +/_ 10mA

OUTPUT IMPEDANCE 0.1 Ohm typical

DIGITAL I/O 5V max 10 mA (input) ; 25V @ 500mA max. (16 open collector outputs)

MAINS VOLTAGE 85-260 V AC, 45-65 Hz 50W

EMC CE certification pending

DIMENSIONS 13.3 cm x 42.5 cm x 25.4 cm (5.25” x 16.73” (19” rack) x 10” )

WEIGHT Approximately 9 lb (4.0 kg)

ENVIRONMENT -10 to +40 °C

HUMIDITY Max. 95% relative humidity, non-condensing

Measurement Settings Screen

The Measurement Settings Screen summarizes the operating display mode for each channel. Submenus can be accessed from this screen that allow the user to select from a variety of data display options for each channel. The Bridge option allows the user to select between “Normal” and Minimum, Maximum and Mean mode. Bridge mode permits a selection of different output formats, e.g., scaling the output of the transducer to display standard units of force, pressure, or displacement “cal”, direct transducer output (mv), or the bridge balanced. The output can also be configured to display the unscaled 24 output bits from the ADC.

Excitation Settings Screen

This screen is used is used to set the magnitude of the excitation voltage for each channel. Excitation voltages can be either AC or DC. The AC mode allows the user to choose from 12 preset frequencies which can be set inde-pendently for each channel. The DC mode is adjustable to 10 volts.

Bridge Settings Screen

The Bridge settings Screen lets the user configure each channel as a full wave bridge, half wave bridge or as a straight differential amplifier. The maximum input signal voltage range a can be adjusted in order to achieve maximum analog to digital conversion resolution. Lowpass (bandwidth ) filter settings can also be adjusted to reduce unwanted noise. The user can assign a unique name for each channel. The

frequency response characteristic (attenuation ) of the channel is provided at 50 Hz, 60 Hz and at a user defined frequency.

See Myobath — page 48.



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Myobath II

System OverviewFor more than 100 years pharmacologists and physiologists have been studying cardiac and smooth muscle in tissue baths. WPI’s multi-channel isolated tissue bath system was developed over a period of several years in collaboration with top cardiovascular research scientists. The result is a feature packed, cost-effective system for the in vitro study

of vascular and non-vascular smooth muscle. Tissue holders are also available for cardiac muscle and other tissue preparations.

The Myobath is available in 2-, 4- and 8-channel configurations. Its modular design allows up to four individually mounted baths to be studied simultaneously. Multiple systems can be linked together if more bath setups are required. The convenient compact bench-top

design of Myobath II enables the system to be readily located even in tight quarters. WPI’s Custom tailored systems are sold complete with every hook, every hanger and all of the tubing and fittings you need to get right to work. We provide well documented, clear assembly/operation instructions and free telephone support in case you encounter a problem.

Multi-Channel Tissue Bath System



SYSTEMSMYOBATHII-2 Myobath II, 2-channel (choose 2 each — baths, positioners, tissue holders — from price tables on following pages) MYOBATHII-4 Myobath II, 4-channel (choose 4 each — baths, positioners, tissue holders — from price tables on following pages) MYOBATHII-8 Myobath II, 8-channel (choose 8 each — baths, positioners, tissue holders — from price tables on following pages)



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RTissue Bath

WPI offers five different tissue baths: 2 mL, 5 mL, 10 mL, 25 mL, and 50 mL. This range is sufficient to cover a broad variety of applications including those involving smaller vessels and tissues from mice or other small animals.

Each tissue bath has 6 ports. The baths are jacketed to maintain the temperature of the perfusate in the center well. The jacket has in inlet and an outlet. A separate inlet with a ceramic frit is used to aerate the perfusate. The frit is designed to produce smaller bubbles that are

less prone to strike the tissue or supporting hangers hard enough to cause a perturbation in the force signal. The bath is also provided with a drain port at the bottom of the chamber, an overflow port and a fill port positioned at the top. The overflow insures that perfusate delivered into the bath will not overflow the chamber and contaminate the work surface. It is also critical for automated fill and drain operations using the optional valve bank and valve controller.

Perfusion and Manifolds

AerationA 6-channel aeration manifold, designed to regulate the flow of O2/CO2 mixtures, is mounted on the top of the center stainless steel post. Four regulators (needle valves) are used to regulate the flow of O2/CO2 to each individual tissue bath. The remaining two regulators are used to supply O2/CO2 to the buffer reservoir and dissection dish. The buffer reservoir holds warmed, oxygenated buffer solution to fill the tissue baths. The tissue baths and the buffer reservoir are jacketed and require a heating and circulating pump to maintain the desired temperature (e.g., WPI #500787).

Drain ManifoldsOne five-port drain manifold with mounting clamp is supplied with each two channels ordered. Each channel bath connects its drain and overflow ports to the manifold. Five ports are sufficient to accommodate two baths. The fifth port is connected to waste. The advantage of using a manifold is that vacuum may be optionally connected to the waste port, greatly reducing drain times.

Stands and clampsThe skeleton of WPI’s tissue bath system is constructed of a system of bases, rods and clamps. The modular design allows systems of almost any number of baths to be created and, systems can be expanded at a later date. The base system is comprised of a weighted base, 4 steel rods, a stabilizing bar and a jacketed 1-liter perfusate reservoir with stand and support.

Positioners

Micrometer Positioner/TensionerThe optional Micrometer Transducer Positioner (#47700) can be used to precisely tension tissues and keep track of the exact position or extension of the tissue. The y axis position can actually be read out from the positioner and fed to a channel of the data system so length and tension can be simultaneously plotted. The user should specify P (for transducer position) or M (micrometer head) in the name.

Classic leadscrew driven positioner/tensionerThe Transducer Positioner (#502198) holds any transducer firmly on a stand or rack. The transducer carrier is slotted to accommodate transducers that have their connecting

cable exiting from the handle. The device allows the vertical position of the transducer to be easily adjusted with excellent resolution. The compact

design keeps the distance between transducer and mounting pole to a minimum. This results in better

stability and less vibration pickup. A release button on the positioner allows it to slide freely up or down for quick coarse adjustment. Fine adjustment is achieved by

turning the knob on the top.

The positioner can be mounted on a pole with a diameter of up to 12.7 mm (1/2-in.). Transducer handles from 7 mm to 11 mm can be mounted

on the holder block. Sensor cables can be fed through the open slot in the clamp so that transducers with both ends larger than 11 mm can still be mounted. Total end-to-end travel is 85 mm.

Automated Filling and DrainingIn the standard configuration, both the filling and draining are fed by gravity. Optional automated control of filling and draining is available for four-bath configurations. This option provides efficiency and statistical stability in multi-channel experiments by reducing human interaction. In this configuration, each channel has a solenoid valve control in the filling connection and a solenoid valve control in the draining connection. While filling is still fed by gravity, draining is enhanced with a built-in pump. The solenoid valves are controlled with SVC108 controller, which has 9-pin D-Sub connector for external TTL control signals. The 4-channel auto filling and draining system 472-4AFD includes two 4-channel solenoid valves and one 8-channel solenoid valve controller.



Baths 47262 Tissue Bath 2 mL47263 Tissue Bath 5 mL47264 Tissue Bath 10 mL47265 Tissue Bath 25 mL47266 Tissue Bath 50 mL

FILLING & DRAIN OPTIONS472-4AFD 4-channel Auto Filling and Draining SystemSVC108 8-channel Solenoid Valve Controller472-4SV 4-channel Solenoid Valves

Positioners47700 Micrometer Positioner/Tensioner502198 Classic Lead Screw driven positioner/tensioner

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Glass Hook (G)The glass hook is a universal design utilizing made from a glass rod terminating in an S-shaped glass hook. Stainless steel hooks or fine wire hangers are used to secure the tissue between the glass hook and the force transducer. The glass rod has a diameter 6.5mm and the hook is 2.5 mm away from the side of the glass rod.

AS Holder (#47030)The AS holder is used for mounting atrial muscle preparations. This holder is equipped with stimulation electrodes. The tissue is mounted onto the two S-shaped hooks (made from 301/2 gauge needles), The S-hooks measure 2 mm in diameter on both ends with a sharp tip on one end where tissue is pieced. The two point stimulation platinum (Pt) electrodes on the holder are 2.54 mm (0.1in.) apart.

V Holder (#47040)The V holder is used to mount vascular rings. The bottom hook consists of a U-shaped wire. The top hook is an L-shaped wire. The tissue is mounted as shown. The hooks are made from 0.48 mm (0.019 in.) stainless steel wire. The U-shaped hook measures 6.35 mm (0.25 in.) in base.

VS Holder (#47050) V Holder with Stimulation

The VS holder uses the same tissue-mounting mechanism as the V holder, but has two 5 mm flexible platinum stimulation electrodes, which can be positioned around the tissue. The stimulation connector and electrodes are designed to be replaceable. The VS holder can only be used in the 25mL or 50mL baths.

B Holder with Stimulation (BS)The B holder is designed for custom-made hooks. The bottom tissue hook is secured with a nylon screw. Stainless steel wire (0.56 mm or 0.022 in.) provided in the startup kit can be used to make the top hook that connects to the strain gauge. Platinum electrodes for point stimulation are identical to those on the AS holder.

Micrometer allows fine adjustment (0.01-mm) of tension on tissue sample.

Transducer adapter

sleeve

Force transducer

Electrical connection from stimulation manifold

point stimulation electrodes

Heated water outlet

Overflow

Heated water inlet

Tissue bath

Tissue is held under tension between holder and force trans-ducer. S-hooks (2-mm high, of 30-ga. wire) are used for atria and other tissues.

Oxygen aeration inlet with built-in

bubbling frit

Drain

Tissue Holders The devices that actually hold tissue for experimentation in a tissue bath are critical to success. They need to be robust, appropriately sized and well thought out in terms of the attachment to the tissue. Since many different sizes and types of tissue can be used in the study of smooth muscle, variety is also critical. WPI offers 10 different application specific tissue support devices.

Five types of tissue holders are available for 10-50 mL organ baths and five types of Miniature Tissue Holders are available for smaller tissue preparations.

In many applications it is desireable to deliver electrical stimulation to the tissue. WPI offers a series of tissue holders with built in stimulation electrodes. Connection to the stimulation device is accomplished with low mass connectors and wire. These ultimately connect to BNC connectors mounted in the stabilizing par of the frame. Connections to isolators or stimulators can then be made with standard coaxial cables.





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Micrometer

Connect to Microsensor Cable #15810

Held in micro-manipulator

10 mm

3 mmcarbon fiber

FORT-100 Transducer

Bath≥ 10 mL

For vessels as small as 500-600 microns (I.D.)

Stainless steel wire (250) µm in diameter)

Micro Tissue Holder MV100 (#47110)

Pt Spiral stimulation electrodes

Stainless steel (250 µm in diameter)

For use with FORT100 transducer and >5 mL bath

Stainless steel(1mm in diameter)

Vessel (> 2 mm

ID)Microvessel (7500-

600 µm (I.D.)

Micro Tissue Holder MVS100 (#47120)

Microtissue holders (MH1, MHS1, MV100,

MVS100 and MVS200) are not supplied with the Myobath system

and must be purchased separately.

Micro Tissue HolderMVS200 (#47130)

L-Shaped Free Radical Sensor (nitric oxide, hydrogen peroxide, oxygen)

Point stimulation electrodes (MHS 1 Only)

2 mL or 5 mL baths

Micro Tissue Holder MHS1 (#47075)

— For force measurments smaller than 5-10 g

Micro Tissue HoldersWith the wide use of transgenic mice in studies of this kind, the need for tissue support mechanisms capable of handling the extremely small size of vessels and muscle strips harvested from small animals is paramount. WPI offers five micro-tissue holders for use with micro tissue strips, micro vessels and other tissues where the force measurements are less than 10 g.

Micro tissue holders are designed to be used with the 2 mL and 5 mL volume tissue baths and FORT 25 transducer. Microtissue Holders MH1 and MHS1 are designed for muscle strips as small as 0.5 mm in width and 5 mm in length. The muscle strip is held between two miniature vessel clips with a clamping pressure of 60 grams. These holders are most suitable for measuring isometric force of less than 5-10 grams. MHS1 differs from MH1 only in that it has an in-built stimulation circuit and electrodes.

Micro vessel holders MV100, MVS100 and MVS200 require a tissue bath larger than 5 mL and the use of FORT 25 or comparable transducer. These holders are designed for studying tension in vascular rings. When used with the micrometer driven tensioner/positioner they can also be used for measuring the lumen diameter of vessel preparations. The MV100 and MVS100 can be used for micro vessel preparations as small as 500-600 microns (ID). MVS200 is designed for larger vessels (> 2 mm ID). MVS100 and MVS200 feature specially designed spiral stimulation electrodes fabricated from 250-micron diameter platinum wire. The two-and-a-half turns of each spiral electrode span appropriately 4-5 mm. The positions of the spiral electrodes can be adjusted with respect to the tissue placement, a feature that is very useful in controlling stimulus intensity.



Holders 503201 glass Hook47040 Vascular Hook Style V47050 Vascular Hook with Stimulation Style VS47030 Atria Holder with Stimulation Style AS47060 Blank Holder with Stimulation Style BS47110 Mini Vessel Holder Style MV47120 Mini Vessel Holder with stimulation, size 1 - Style MVS147130 Mini Vessel Holder with stimulation, size 2 - Style MVS247070 Mini Tissue Strip Holder Style MH147075 Mini Tissue Strip Holder with Stimulation - Style MHS1

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Record dose response.

Transducers

FORT25The rigid-lever force transducers transform applied force into proportional voltage. Using balanced strain gauges, FORT transducers produce linear output voltage vs. applied force input with very little deflection. The MyOBATH II system is designed to use together with WPI’s force transducers, including the FORT100 (full scale, 100 g) and FORT25 (full scale, 25 g). These transducers can be directly connected to either the 4-channel Transbridge™ amplifier or the more advanced 8-channel Bridge8 or digital DBA8000 amplifiers. Bridge8 and DBA8000 are the preferred amplifiers for small vessel applications because they provide additional noise filtration (0.03-10Hz). In this way, the noise produced by increased sensitivity of the FORT25 transducer can be significantly attenuated.

FORT10-100The FORT10-100 is a high sensitivity dual-range research grade force transducer designed to measure forces in the 0.005 to 10 gram and 0 to 100 gram ranges. FORT10-100 exhibits excellent isometric properties. In addition, its unique optical technology makes it resistant to drift and offers intrinsically low noise characteristics. FORT10-100’s on-board amplifiers reduce gain and adjustment requirements on recording amplifiers to which it may be connected.

Grass/Narco/Kent/HarvardWPI positioners, baths, amplifiers, and data systems are completely compatible with sensors from grass, Narco, Kent and Harvard.

Amplifiers

TransbridgeAll of the transducers above can be directly connected to the 4-channel Transbridge™ amplifier. This basic device offers 4 channels of variable gain transducer conditioning. Each channel has x1 x10 x100 and x1000 gain and offset control. Bandwidth is fixed at 1kHz.

Bridge 8/IsoDam Rack

Bridge8 is the preferred amplifier system for microvessel applications because it provides additional signal filtration (0.03-10Hz). In this way, the noise produced by more sensitive tansducers such as the fort 25 can be significantly attenuated.

Data Systems

Hardware — Lab-TraxAlthough the WPI tissue bath can be configured to work with any data acquisition system, WPI’s Lab-Trax is the preferred recording platform. Each channel in the Lab-Trax contains its own 24-bit analog-to-digital converter, an independent stimulator output and a built-in variable gain bridge amplifier. In addition, 8 programmable digital outputs are provided. These are easily configured and are used to control the automated filling and draining of individual baths. By combining all

of these functions into a 4- or 8-channel device, cost goes down and performance goes up. The low-profile box fits virtually anywhere and because all functions are integrated into the same instrument, system noise is low — and best of all, the wiring hookup is reduced to plugging in transducers and power. The bundled Data-Trax software includes dose response software that can manage multiple runs, calculate ED-50 and draw Schild plots.

SoftWare — Data-Trax Although the bundled Data-Trax software can be seen as a general purpose digital chart recorder, there are many features that make recording and analysis of isolated tissue bath data easier and less time consuming.

The Data-Trax software allows you to easily convert voltage to real units, such as grams or mmHg. The display allows you to toggle between a preferred y-axis scaling (between 0 and 6 grams, for example) and the “best view” as determined by the AutoScale feature. Alternately, you can zoom-in or zoom-out by factors of two with a single mouse click. In a similar fashion, x-axis scaling can be expanded or compressed by factors of two with a single click. Where multiple channels are being viewed simultaneously the user can define additional “Views”of the data. For example in addition to the main or default display showing all channels, the user might create additional views, each showing only a few channels to allow a better online presentation of the data

While recording, you can mark your data with text messages. These marks are searchable, and appear in the field of data to which they apply. Marks are time locked to the record and can be used to “go To” any marked region of the data. They are also used post recording by the DOSE RESPONSE software to calculate ED50 values and Draw Schild plots.





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Automark and extract maximum response values.

Use a custom script to build dose response graphs ...

... and Shild plots.

Data-Trax also controls the built in stimulator in The WPI-118 and T-series recorders. Pulse protocols can be created in advance, stored and delivered automatically or on command. Pulse durations, frequencies and amplitudes can be changed as the protocol is being delivered. The software also offers full control of digital I/O lines that can be used to count events, or control devices in the lab environment such as valves or injectors. Using WPI’s AUTODRAIN together with the Data-Trax protocol generator, the entire process of draining, filling, and drug delivery can be automated.

ABEThe dose response software provided is based on ABE, an open source freeware Module written by gordon Webster. The package as provided has been modified to accept Data trax formated data and provide a smooth transition from recording to analysis

ABE is written in Python 2.2 and uses the Tkinter graphic library to generate a friendly graphical user interface (gUI) for manipulating and visualizing the data.

For the data modeling, ABE uses the nonlinear regression routines from the Scientific Python (SciPy) and Numeric libraries as well as the polyfit function from Raymond Hettingers Public Domain Matfunc module for computing the fitted polynomial coefficients from the supplied data. Functions for computing the derivatives of the fitted polynomials, solving polynomial roots and estimating the initial parameters for the nonlinear regression were added by the author and are included in the body of the main Abe module. In addition to the libraries of the standard Python distribution therefore, only the SciPy, Numeric and Matfunc modules need to be included in the Python path for ABE to be run. These are provided on the Data-Trax install disk

Options

Heating CirculatorIdeal for controlling temperatures of external systems. This heating circulator features a polyphenyleneoxide (PPO) construction with a powerful 17 liter per minute flow rate pump for optimum heat exchange with a 12 mm hose. The working temperature range is 25-100°C. A tap water cooling coil (with a working temperature range of 20-100°C) is fitted as a standard feature. The unit comes with a stainless steel cover. The 115 V model is equipped with a 1200-watt heater and the 230 V model comes with a 2000-watt heater.

The bath has a volume of 5 liters and an opening of 13 x 17 x 16 cm. The overall dimensions of the unit are 16 x 33 x 36 cm. Shipping weight: 22 lb (10 kg).

Bath made from polyphenylene

oxide (PPO).

Stainless steel bath cover.

Menu-driven control module allows easy temperature setting, correc-tion and display. Temperature accuracy of ± 0.02°C.

Fluid ports for external cooling.

Heated bath inflow and

outflow



500787 Haake DC10-P5/U Circulating Bath (115 V) 500788 Haake DC10-P5/U Circulating Bath (230 V) 500789 Haake DC10-P5/U Circulating Bath (100 V)

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5454

EVC4000 SPECIFICATIONSPREAMPLIFIER InputResistance 1012Ohms InputLeakageCurrent 100pA,max. MaximumInputVoltage ±15voltsVOLTAGECLAMP PanelDisplay ±200mV±0.1mV ClampVoltage/ExternalInput 100mVperVolt RangeofVoltageElectrodes ±32Volts Max.ClampVoltage ±100mV FluidResistanceCompensation 0to1000OhmsCURRENTCLAMP PanelDisplay ±999µA±1µA MaximumClampCurrent ±1milliampere CurrentClampOutput 1µA/mVDISPLAYRESOLUTION Voltage 0.1mV Current 1µADIMENSIONS 18.25x7.2x9.6in. (46x18x24cm)SHIPPINGWEIGHT(EVC4000-4) 26lb(12kg)

EVC4000

Multi-Channel Voltage / Current Clamp

EVC

40

00

-4(c

ompl

ete

four

-cha

nnel

sys

tem

)

Afour-channelVoltage/Currentclampapparatus,EVC4000employsthevoltageclamptechniquetomonitormembranepermeabilityasafunctionofmembranevoltageorappliedchemicals.WhencombinedwithWPI’spatentedEKCandEKVcartridgeelectrodes,EVC4000canefficientlyvoltageorcurrentclampuptofoursamplemembranessimultaneouslyusingsafemoderatevoltagesonthecurrentwireleads.Thesuperiordesignofthecartridgeelectrodesmakes100-voltcurrentexcursionunnecessary,sothissafe,low-voltagesystemiseasiertoadjustanduse.Extremelystableandaccurate,eachmodule,withitscompanionpreamplifier,canoperateindependentlyinoneofthreedifferentmodes:Voltage Clamp(VC),Current Clamp(CC),orOpen Circuit Potential(PD)measurement.EVC4000canbecontrolledfromthefrontpaneloftheinstrumentorfromcomputergeneratedcommandsappliedattherearpaneloftheinstrument.AfeatureuniquetoEVC4000isanelectronicpotentiostatinthepreamplifierboxthatmaintainstheserosalelectrodeinvariantpotentialatzerorelativetosystemground.Thepreamplifierapparatusactivelymaintainsonesurfaceofthetestmembraneclosetogroundpotentialunderalloperatingconditions.

More channels and a wider range of voltage clamp commands than WPI’s classic DVC-1000. The superior design of the cartridge electrodes makes 100-volt current excursion unnecessary, so this safe, low-voltage system is easier to adjust and use.

ReferencesW. K. MacNaughton(2000)“Roleofconstitutivecyclooxygenase-2inprostaglandin-dependentsecretioninmousecolonin vitro.” Journal of Pharmacology and experimental Therapeutics293,2,539-544


UK: Tel: 01438-880025•Fax: 01438-880026•[email protected]: Tel: 030-6188845•Fax:030-6188670•[email protected]

EVC4000-4 4-ChannelVoltageClamp&preamps(shownabove)

EVC4000-3 3-ChannelVoltageClamp&preampsEVC4000-2 2-ChannelVoltageClamp&preampsEVC4000-1 1-ChannelVoltageClamp&preamp


OPTIONAL ACCESSORIESEVC4000 ReplacementVoltageClamp&EVC3PreamplifierEVC3 ReplacementPreamplifierModuleEK1 UssingElectrodeKit(2voltage,2current)EKV ExtraUssingVoltageElectrode(each)EKC ExtraUssingCurrentElectrode(each)2851 BNCCable



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Acquiring Data from the EVC4000 Current/Voltage

TheuseofLab-TraxhardwareandbundledData-Traxsoftwarenotonlyprovidesaneasy,lowcostsystemtorecordepithelialvoltageclampdata,butalsoprovidespowerfultoolsforexperimentalcontrolandanalysis.Digitaloutputscanbeusedtochangetheclampingmodeandstate(Current/Voltageorfromzerotoapresetvalue).SincethereisnonaturallimittohowlongyoucanrecordwithData-Trax,itisideallysuitedto

longexperiments.Inaddition,searchablemarksandapoint-and-clickinterfacetocontrolthex-axisscalingmakenavigationoflongrecordseasy.

Analyticalroutinesdesignedtofollowconductancechangesinthemembraneareeasytocreateandcanbeadjustedaccordingtoexperimentalrequirements.

Hardware

WPI-118(seepage71)offersthehigherspeedrecordingnecessarytoperformmembranesurfaceestimations.Ithas8digitaloutputsforcontroland2independentDACsthatcanbeusedtocommandclamplevel.

Lab-Trax 4/16(seepage70)providesfourchannelsofrecordingatanaffordableprice.TheS-seriesrecorderhas2/4digitaloutputsanddoesnotofferaDAC.


World Precision Instruments • Tel:941-371-1003 • Fax:941-377-5428 • E-mail:[email protected] • Internet: www.wpiinc.com

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5656

UssingChamber

Ussing System

For electrophysiological investigation of epithelial transport

Complete Ussing System includes stand, glass reservoir, electrodes, Ussing chamber and tubing (EVC3 preamp and ringstand clamp not included—see page 54).

Twosmallchamberswithrectangularopenings aredesignedfortubu-lartissuefromsmallanimalssuchasthemouseintestinaltractmem-brane(CHM6)andratintestinaltractmembrane(CHM7).Therectangu-laropeningmorecloselymatchestheshapeofthetissuethanwouldacircularopening,significantlyincreasingthemembraneareaavailablefortesting.Thelargermembraneareaincreasesthetransportrateoflowpermeabilitychemicals;italsoreducestheelectricalresistanceofthesystemforeasiercurrentclamping.

Optional DrainsDrainsmaybeaddedtoUssingchamberstoallowquickandcompleteevacuationofradioactiveortoxicsubstances.Tohavedrainsaddedatthetimeoforder,adda“D”tothepartnumber(suchas“USS1LD”);$100willbeaddedtothecostofthechamberorsystemyouorder.

Cartridge ElectrodesTheElectrodeKitcontainsfourvoltage/currentelectrodes,plusfourluer-tippedcartridges.Electrodesarethreadedandscrewsecurelyintotheendofeachcartridge.Theluertipthenplugssecurelyintothelueropen-ingsofthechamber.Thecablefromeachelectrodeterminateswitha2mmpinwhichmaybepluggedintovoltage/currentclampssuchasWPI’sDVC1000orEVC-4000.

Theminiatureelectrode-gelcartridgeisasmallplastictubewithamaleluertipidenticaltothoseatthetipofhypodermicsyringes.Thetubemaybefilledwithdifferentgelmaterials;agariscommonlyusedbutothergelmaterialsmayalsobesatisfactory.

WPI’sUssingSystemoffersresearchersaquick,effectivemeansofmakinglow-resistanceelectricalconnectionstotheUssingchamberwithoutneedoflongagarbridgesorCalomelhalf-cells.Ag/AgClhalf-cellsscrewintoshorttubeswhichplugfirmlyintoplaceinthechamber’sluerports.Thesedirect-connectelectrodeseliminatetheinconvenienceandexpenseofCalomelhalf-cellsinopenliquids.TheSystemincludesoneUssingChamber(eightsizesavailable),SupportStand,ElectrodeKit,GlassCirculationReservoir(twosizesavailable),andaTubingstart-upkit(25feetof0.375-in.tubing,10feetof0.156-in.tubing,plusfourmaleluerfittings,twocompressorclamps,oneY-connector,andoneclip).Six-teenpossiblesystemconfigurationsarelistedatright.Componentsarealsoavailableseparately.(Preamplifierinphotonotincluded.)

Ussing ChambersWPI’sclassicalUssingChambersarewellestablishedperfusionchambersthatareeasytooperate,easytocontroltemperature,andeasytocleanafteruse.Hundredsofthemareuseddailybyscientistsinthefield.UssingChambersaremachinedfromsolidacrylicwitheightentryportsforfluidlines,electrodes,oragarbridges.Foreasy,leak-freeattachmentoftubingandelectrodes,alleightportsareluertype.Thefourportsforvoltageandcurrentelectrodesarerecessedtopreventformationofairbubblesinthechamber.Thefluidcompartmentsineachsideofthechamberareseparatedbytheepithelialmembranebeingstudied.Sharpstainlesssteelpinsononesideofthechamberholdthemembraneinpositionandmatewithholesintheoppositechamberinterface.(IntheCHM4,tissueisheldbyanO-ringinsteadofpins.)

The CHM5 chamberadaptstheCostar Snapwell,acellcultureinsertformonolayercellculture,intoWPI’s“classical”epithelialvoltageclampsystem.Untilnow,classicalUssingChambershavenotbeenwidelyusedformonolayercellcultureinsertsbecausemostinsertshaveaverydeepprofile,limitinggoodfluidperfusionatthesurfaceofthemembrane—andlimitingvoltageelectrodesfrommeasuringthepotentialclosetothesurfaceofthemembrane.CHM5solvestheseproblems:Perfusionfluidisintroducedintothechamberatananglesothatitwillflowdirectlytothesurfaceofthemembrane.Thevoltageelectrodeisalsoinsertedintothechamberatananglesoastoreducethedistancebetweenthesurfaceofthemembraneandtheelectrode.

U.S. Patent N

o. 4,912,060

EKV and EKC Cartridge Electrodes

UssingChamber

Glasscirculation reservoir #5210

Glasscirculation reservoir #5210

● Direct connect low-resistance electrodes

● Simplified operation, easy to control temperature and clean after use

● Luer type leak-free attachement of tubing and electrodes

● Recessed electrode ports to avoid air bubble formation

● Secure membrane holding by sharp stainless steel pins or O-ring

● Specialized chamber adapts cell culture insert (Costar Snapwell) for monolayer cell culture

● Chambers with rectangular openings for tubular tissues from small animals





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Circulation ReservoirsHand-blownborosilicateglass,withjacketedcham-bersfortemperaturecontrol.Availableintwosizes—#5210holds20mLperside,and#5362(atleft)holds10 mLperside(usefulwhenexpensivechemi-calsareinvolved).Reservoircondensercapspreventairbubblesandturbulenceinfluidreservoirs.

Water BathTheHaakeDC10-P5/UConstantTemperatureWaterBathhasbeentestedatWPIandfoundtobeidealforWPI’sUssingsystem.Itcan

providesimultaneouswarmingforupto16systems.Menu-drivencontrolmoduleallowseasytempera-turesetting,correctionanddisplay,withtemperatureaccuracyof±0.02°C.

57

Suture quality pins minimize tissue damage

Suture quality pins minimize tissue damage Fluid in

Luer ports allow easy fitting of fluid lines and electrodesLuer ports allow easy fitting of fluid lines and electrodes

CHM1

12 mm

17 mm

12 mm

CHM2

CHM4

CHM5

9 mm

12 mm

4 mm

6 mm

CHM3

CHM6 CHM7

13.5 mm

18.5 mm

14.5 mm

5 m

m

7 m

m

16.5 mm30 mm

7 m

m

9 m

m

32 mm

CHM8

5.5 mm

0.5 mmpins

4 mmReservoir openings and

pins

Clear acrylic chambers let you see your experiment in progress . . .Clear acrylic chambers let you see your experiment in progress . . .

Fluid in

Fluid outFluid outVoltageElectrodeVoltageElectrode

CurrentElectrodeCurrentElectrode

New CHM8 Chamber

AlignmentDotsAlignmentDots

GuidePins

GuidePins

Optional drain for hazardous material also available

Optional drain for hazardous material also available

NewtoourfamilyofUssingchambersisonedesignedspecificallyfortheMilliporeMillicell12mmtissueinsert.ThisisthefirstchamberonthemarkettocompensateforthedeepprofileoftheMilliporeinsert.Thisdeepprofilemakesitdifficulttobringeitherthevoltagemeasuringelectrodesorfluidflowtothesurfaceofthetissuemembrane.Ourinnovativedesignvirtuallyeliminatesthe“unstirredlayer”offluidwhichwouldotherwiseslowdowntheresponseofperfusionexperiments.Inaddition,voltagemeasurementsarebroughtrightuptothesurfaceofthetissuemembrane,facilitatingvoltageclamping,currentclampingandTEERmeasurements.

Ussing chamber for Millipore Millicell 12mm tissue insert

CHM1 CHM2 CHM3 CHM4 CHM5 CHM5M12 CHM6 CHM7 CHM8 (Medium) (Small) (Large) (ExtraSmall) (Snap) (MilliCell) (Rect.,Small) (Rect.,Large) (ExtraSmall)

Reservoir Opening 12mm 9mm 13.5mm 4mm 12mm 10mm 5x14.5mm 7x30mm 4mm

Half-Chamber Volume 1.0mL 0.75mL 1.2mL 0.5mL 1.7mL 1.25mL 0.8mL 5.5mL 0.5mL

Pin Circle Diameter 17mm 12mm 18.5mm 6mm* N/A NA 7x16.5mm 9x32mm 5.5mm

Assembled chambers are 101.6 mm (4 in.) long. *O-ringdiam.



USSING SYSTEMS, LARGE RESERVOIRUSS1L Medium Chamber, Stand, Reservoir, Electrodes, TubingUSS2L Small Chamber, Stand, Reservoir, Electrodes, TubingUSS3L Large Chamber, Stand, Reservoir, Electrodes, TubingUSS4L Extra Small Chamber, Stand, Reservoir, Electrodes, TubingUSS5L Snap Chamber, Stand, Reservoir, Electrodes, TubingUSS5M12L Millipore Chamber, Stand, Reservoir, Electrodes, TubingUSS6L Small Rectangular Chamber, Stand, Reservoir, Electrodes, TubingUSS7L Large Rectangular Chamber, Stand, Reservoir, Electrodes, TubingUSS8L Extra Small Chamber, Stand, Reservoir, Electrodes, Tubing

USSING SYSTEMS, SMALL RESERVOIRUSS1S Medium Chamber, Stand, Reservoir, Electrodes, TubingUSS2S Small Chamber, Stand, Reservoir, Electrodes, TubingUSS3S Large Chamber, Stand, Reservoir, Electrodes, TubingUSS4S Extra Small Chamber, Stand, Reservoir, Electrodes, TubingUSS5S Snap Chamber, Stand, Reservoir, Electrodes, TubingUSS5M12S Millipore Chamber, Stand, Reservoir, Electrodes, TubingUSS6S Small Rectangular Chamber, Stand, Reservoir, Electrodes, TubingUSS7S Large Rectangular Chamber, Stand, Reservoir, Electrodes, TubingUSS8S Extra Small Chamber, Stand, Reservoir, Electrodes, Tubing

* Add EVC4000 at reduced price when buying Ussing System with equivalent number of channels

EVC4000-1 1-Channel Voltage Clamp & Preamps EVC4000-2 2-Channel Voltage Clamp & Preamps EVC4000-3 3-Channel Voltage Clamp & Preamps EVC4000-4 4-Channel Voltage Clamp & Preamps

System components also available separately:xxxxD Drain option (add “D” to part number of chamber or system) CHM1 Medium ChamberCHM2 Small ChamberCHM3 Large ChamberCHM4 Extra Small Chamber with O-Ring SealCHM5 Snap Chamber (fits Costar Snapwell cups) CHM5M12 Millipore Chamber (fits Millipore 12 mm insert) CHM6 Small Rectangular ChamberCHM7 Large Rectangular ChamberCHM8 Extra Small Chamber with Mounting PinsEK1 Ussing Electrode Kit (2 voltage, 2 current) EKC Extra Ussing Current Electrode (red) (each) EKV Extra Ussing Voltage Electrode (blue) (each) DRIREF-L Reference Electrode w/ Luer Tip5210 Large Glass Circulation Reservoir, (20 mL per side) 5233 Replacement Stopper for 52105362 Small Glass Circulation Reservoir, (10 mL per side) 5361 Replacement Stopper for 53623955 EKV Cartridges, 35 mm (pkg of 12) 3960 EKC Cartridges, 58 mm (pkg of 12) 3669 Tubing Kit (flexible hose and luer fittings) 3579-20 Replacement luer fittings for tubing connections (pkg of 20) 5153 Support Stand

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5858

Two-Channel Voltage / Current Clamp

WPI’sDVC-1000Two-ChannelVoltage/CurrentClampiswell-knownandwidelyusedforstudyingepithelialtissues.EachDVC-1000consistsoftwoseparateclampinstruments,oneforclampingacontroltissue,anotherforclampingatesttissue.Thisdualclamptechniqueletsyoumonitorchangesinmembranepermeabilityasafunctionofvoltageorappliedchemicalagents.Eitherclampunitoperatesinfivemodes.Voltage clamp:Clampsthetissuevoltagetoagivenlevelanddisplaysthecurrentrequiredtomaintainthatlevel.Amplify: Showsthepoten-tialdifferencebetweenthetwovoltageelectrodes.Current clamp:Letsyoudeliveraconstantcurrentbetweenthetwocurrentelectrodeswhilesimultaneouslymonitoringvoltagechangesatthetissue.Timer: Functionsineithercurrentclamporvoltageclampexperiments,lettingyoucycleautomaticallybetweenzeroclampandapresetclamplevel.Remote:Allowsyoutocontrolclampoperationfromacomputerorotherlogiclevelsource.Smallpreamplifiers(included)whichmountclosetothechamberletyouconnecttovoltageandcurrentelectrodeswithoutlongcablesoragarbridges.DVC-1000alsofeaturesaunique±100Vpowersupplycapableofdeliveringupto1mAofclampcurrent.Eachclampletsyoucorrectforinputoffsetvoltagesandfluidresistanceerror.

DVC-1000 SPECIFICATIONSPROBES INPUTIMPEDANCE 1012Ω LEAKAGECURRENT 100pAmax MAXIMUMINPUTVOLTAGE 10VVOLTAGECLAMP CLAMPVOLTAGERANGE: SETCLAMPPOT ±100mV EXTERNALCOMMAND ±1V COMMANDFACTOR 10mV/mV MAX.CLAMPCURRENT ±1mACURRENTCLAMP CLAMPCURRENTRANGE: SETCLAMPPOT ±1mA EXTERNALCOMMAND ±1mA COMMANDFACTOR 1mV/µA COMPLIANCE ±100VINPUTOFFSETRANGE ±130mVFLUIDRESISTANCE COMPENSATIONRANGE 0-1000ΩTESTCURRENT 10µAto180µAadjustableOUTPUTRESISTANCE 100ΩTIMERRANGE 500msto500seachsideLCDMETER TYPE 31⁄2digitswithNoiselok™ MAX.READING 2000µA,200mVPOWERREQUIREMENTS 95-135Vor220-240V,50/60HzDIMENSIONS 17x8.75x9.5in. (43x22x24cm)SHIPPINGWEIGHT 21lb(9.5kg)

DVC-1000DVC-1000

The FIRST — and still the most popular, with thousands of users around the world!

The FIRST — and still the most popular, with thousands of users around the world!



DVC1000 Voltage/CurrentClampIncludes two DVC3 preamps and one DVC2 dummy membrane.


OPTIONAL ACCESSORIESDVC2 ReplacementDummyMembraneDVC3 ReplacementPreamplifier2935 RackMountKitEK1 UssingElectrodeKit(2voltage,2current)EKC ExtraUssingCurrentElectrode(red)(each)EKV ExtraUssingVoltageElectrode(blue)(each)3485 RingstandMountingKit(seepage7)



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5959

EVOM™

Epithelial Voltohmmeter

EVOM SPECIFICATIONSMEMBRANEVOLTAGERANGE ±199.9mV

INPUTIMPEDANCE >1010ΩRESISTANCERANGES 0To1999Ω,0to20,000ΩACSQUAREWAVECURRENT ±20µAat12.5Hz

POWER One9Valkalinebattery,andsix 1.5ValkalineAAbatteries,supplied

SHIPPINGWEIGHT 3lb(1.4Kg)

● Manual TEER measurement of epithelial cells in 24- and 96-well plates

● Battery powered electrically isolated meter

● AC current avoids adverse effects on tissue

● Compatible with Endohm chambers

● STX2 manual electrode enclosed with meter

● STX2 can be sterilized with EtO, alcohol or a bactericide

TheEVOMwasthefirstinstrumentdesignedspecificallytoperformroutineTEER(TransEpithelialElectricResistance)measurementintissuecultureresearch.Thebattery-poweredEVOMproducesanACcurrentthatavoidsadverseeffectsontissues,whichcanotherwisebecaused,byaDCcurrent—includingelectrodemetaldeposits.TheEVOMhasaneasy-to-readLCdisplayandisalsoavailablewithaBNCrecorderoutput(EVOMX).InputimpedanceoftheEVOMisgreaterthan1010ohm,andresistancerangesfrom0to1999ohm;and0to20,000ohmcanbemonitoredusingtheswitchablerangesettinglocatedontheinstrumentfrontpanel.

OneuniquefeatureoftheEVOMisthatresistancereadingsareunaffectedbymembranecapacitanceandmembranevoltage.Themembranevoltagereadingrangeis±200-mV.EVOMcomescompletewiththepopularSTX2“Chopstick”electrodeset.TheSTX2consistsofafixedpairofdoubleelectrodes,4mmwideand1mmthick.Eachstickoftheelectrodepaircontainsasilver/silver-chloridepelletformeasuringvoltageandasilverelectrodeforpassingcurrent.Thesmallsizeofeachelectrodeisdesignedtofacilitateplacementoftheelectrodesintocellculturewells.STX2canbeusedwithalltissuecultureinsertscurrentlyonthemarket.Formoreaccuratequantitativemeasurementsand/orforlowerresistancemeasurements(e.g.,endothelialtissuecultures)theEVOMcanbeusedinconjunctionwiththeoptionalEndohmchambers.

Trans Epithelial Electric Resistance (TEER)

During the last two decades TEER measurements have become universally established as the most convenient, reliable and non-destructive method to evaluate and monitor the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. Recently there has been a significant surge of interest in introducing a combined

electrode for resistance measurements in the Millipore 96-well PAMPA (parallel artificial membrane permeability assay) plate.

TEER measurement technology, which was first introduced by WPI in the mid-1980’s, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation.



EVOM EpithelialTissueVoltohmmeter (includes STX2 electrode set)

REPLACEMENTS AND ACCESSORIESSTX2 Replacement“Chopstick”ElectrodeSetSTX3 AdjustableTipSpread“Chopstick”ElectrodeSet3993 ElectrodeAdapter(forelectrodeswith2mmpins)500168 4-WaySwitchbox

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CaliCell™

Cell culture cups with synthetic membrane for testing STX electrodes, Endohm and Ussing chambersIttakesalongtimeandalotofworktogrowabatchofcells,soyouwillwanttomakecertainthatyourtestapparatusisfunctioningproperly.TheCaliCellTMprovidesaquickandpositivewaytotestSTXelectrodes,EVOMs,Endohm,andUssingchamber.

TheCaliCellTMisamajorimprovementinTEERelectrodecalibration.Itsmembranemakesuseofouruniqueelectriccurrentconstrictiontechnologytoproduceresistancereadingscomparabletothoseobtainedwithrealcellcultures.TheCaliCellTMdoesnothavetoberefrigerated,andcanbecleanedandsterilizedwithalcohol.Readingswillnotdriftovertimeaslongastheunitiskeptingoodphysicalcondition.

WiththedevelopmentofaHighThroughputScreening(HTS)protocolforfasterdrugdiscovery,anewlineofcellculturefilterplateshavebeenintroducedbyseveralmajorcellcultureinsertmanufacturers.TheseHTSplatesnormallyhaveeither24or96individualcellcultureinserts“bonded”togetherasoneplatesothatitcanbehandledbyarobotapparatus.Inresponsetothesedevelopments,WPIhasdevelopedanautomaticREMSsystemandamanualelectrode,STX100,forTEERmeasurementsusingHTSplates.

STX100’s design is based on the same reliable design principle as the universally used STX2electrode,withseveralimportantmodifications.Thesizeoftheelectrodetiphasbeenreducedto1.5mmtofacilitatepositioningthroughthenarrowerslitoftheHTSplate.TheSTX100electrodeitselfisconstructedusingastrongermaterialforhigherdurabilityandmaximumusageapplications.Thebottomsectionoftheelectrodeisshapedtofitneatlyintothe“keyhole”shapedfilterwell.ThisenablestheSTX100electrodetoproduceincreasedaccuracyandreproducibilityofTEERreadings(±5Ω)comparedtothestandardSTX2.SeveralversionsofSTX100areavailable,designedtofittheCorningCostar24-wellHTSplate,theFalcon24wellHTSplate,theMilliporeMultiscreenCaCo96-wellplate,andBDFalconHTS96-multiwellplate.MeasurementcanbedirectlyperformedwhentheHTSplateisineitheracommonordividedtray,reducingthepossibilityofcontaminationaswellasmechanicaldamagetotheculturedcells.

For use with HTS plates, the EVOMX is modified so that the “Measurement” button stays on while you move the electrode from well to well.

TEER measurements in High Throughput

EVOMX●BNC output for Data Acquisition System●Toggle switch for continuous measurement

STX100 Series Electrodes

● Designed for 24-well HTS plate (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon)

●Improved accuracy down to 5 Ohm●Sterilized with EtO, alcohol or bactericide

EVOMXisamodifiedversionoftheEVOMandisrecommendedforusewithSTX100serieselectrodes.ItsmeasurementswitchandcircuitshavebeenmodifiedtoremainONcontinuously.Thisenablestheusertohaveonehandfreetomovetheelectrodewhileusingtheothertonotethereadings.ABNCrecorderoutputconnectorisprovidedontheEVOMXtopermitdirectrecordingofTEERmeasurementstoachartrecorderorcomputer-baseddata-acquisitionsystem.



EVOMX EVOM with switch adaptation for multi-well testing

CALICELL-12 12mmCalibrationCellforEndohm-6/Endohm-12CALICELL-24 24mmCalibrationCellforEndohm-24

STX100C STX100 for Corning Costar HTS Transwell-24 STX100F STX100 for Falcon HTS Multiwell Insert System STX100M STX100 for Millipore MultiscreenTM CaCo 96 Well Plate STX100F96 STX100forBDFalconHTS96MultiwellPlate STX100C96 STX100forCorningHTS96WellPlate

OPTIONAL ACCESSORIES13685 ModularCable,7ft

13347 ChartRecorderAdapter 2851 StandardBNCCable,6ft(1.8m) 500184 StandardBNCCable,10ft(3m)

See Chart Recorders — page 186



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6161

Also for use with Millicell ERS

Endohm™

For TEER measurement of endothelial cell cultures in individual cups

●Compatible with EVOM and EVOMX●Improved accuracy of 1-2 Ohm●Accommodates 6mm, 12mm, 24mm cups

and Costar Snapwell cup●Sterilized with EtO, alcohol or a bactericide

Non-cellbased96-wellPAMPAassayshavebeendesignedforpredictivedrugcandidatetesting.Toensurethatthedonor/acceptorfluxesarenotduetoporousorunstablehexadecanelayers,thestabilityofthehexadecanemembranecanbetestedattheendoftheincubationperiodbyelectricalresistancemeasurements.ToconductthemeasurementaPAMPAplateistransferredtoMULTI-96.LiketheotherEndohmmodels(designedforindividualcellculturecups),thebaseofMULTI-96isafluid-filledreceptacleforthePAMPAplates,alsoservingasthebottomhalfoftheelectrodepair.Insertingthetopelectrodeintoeachwellallowstheusertoobtaintheresistanceofthesyntheticmembranelayerofthatwell.MULTI-96providesastableandreproduciblereadingoftissuecultureresistance.EVOMXisrecommendedforusewithMULTI-96.

Multi-96Resistance measurement with 96-well PAMPA (parallel artificial membrane permeability assay) plates

Screening (HTS) cell culture filter plates

UsingWPI’sEVOMresistancemeter,Endohmchambersproviderepro-ducibleresistancemeasurementsofendothelialtissueinculturecups.TransfercupsfromtheirculturewellstotheEndohmchamberformeasurementratherthanusinghand-heldelectrodes.Thechamberandthecapeachcontainapairofconcentricelectrodes:avoltage-sensingsilver/silverchloridepelletinthecenterplusanannularcurrentelectrode.Theheightofthetopelectrodecanbeadjustedtofitcellculturecupsofdifferentmanufacture.Endohm’ssymmetricallyapposingcirculardiscelectrodes,situatedaboveandbeneaththemembrane,allowamoreuniformcurrentdensitytoflowacrossthemembranethanwithSTX2electrodes.Thebackgroundresistanceofablankinsertisreducedfrom150Ω(whenusingWPI’shand-heldSTX2electrodes)tolessthan5Ω.With

Endohm’sfixedelectrodegeometry,variationofreadingsonagivensampleisreducedfrom10-30ΩwithSTX2electrodes(dependingontheexperi-enceoftheuser)to1-2.Comparedwithotherresistancemeasurementmethods,EndohmwithEVOMoffersamuchmoreconvenientandeconomicsolutionto“leakytissue”measurement.Becauseoftheuni-formdensityoftheACsquarewavecurrentfromEVOM,errorsowingtoelectrodepolarizationormembranecapacitancearelargelyeliminated.EndohmtogetherwithEVOMoffersthemostaccurateandeconomicalendothelialohmmeternowavail-able.Todate,cupsfromCostar,Millipore,ICNBiomedicals,andFalconhavebeentested.EndohmchambersmaybesterilizedwithEtO,alcoholorabactericide(alsosee:Cidex,Microsur-gerysection);notautoclavable.



ENDOHM-6 Endohmfor6mmculturecup(24wellsperplate)ENDOHM-12 Endohmfor12mmculturecup(12wellsperplate)ENDOHM-24SNAP Endohm for 24 mm & Costar Snapwell™ cup (6 wells per plate)

Requires EVOM or EVOMX to operate

MULTI-96 EndohmforMilliporeMultiScreen96-wellPAMPAplate

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Automated TEER Measuring SystemAutomated TEER Measuring System

TheREMS AutoSamplerautomatesmeasurementsofelectricalresistanceoftransepithelial,transendothelialorCaco-2cellmembranesbeinggrowntoconfluenceonmicroporousfiltersofhighthroughputscreening(HTS)24-and96-wellmicroplates.ItisaPC-controlled,tissueresistancemeasurementsystemthatoffersreproducibility,accuracy,flexibilityandease-of-operationforthiskindofmeasurement.Automatedmeasurementoftissueresistanceincellculturemicroplatesprovidestheimportantadvantagesofspeed,precision,decreasedopportunityforcontaminationandtheinstantavailabilityofmeasuredresistancedataonacomputer.Thesemeasurementsareusefulinapplicationssuchasdrugbioavailabilitystudiesandstudiesonthemechanismsofdrugtransport.

ThemaincomponentsoftheREMSAutoSamplerinclude:theroboticsamplerthatmovestheelectrodeovereachwellofthemicroplate,theelectrodewhichislocatedontheroboticarm,abaseplateforthe24-and96-welltray,aWindows-baseddataacquisitioncard,theREMSinterfaceunitandtheREMSsoftwaretooperatethesystemonaWindows-basedcomputer.

TheREMSAutoSamplerautomatesTEERmeasurementspreviouslymadewithWPI’sEVOMEpithelialVoltohmmeter.Automatedtissueresistancemeasurementsupto20kΩcanbeperformedon24-or96-wellHTSmicroplates.MicroplatespresentlysupportedincludetheCorningCostarHTSTranswell-24,FalconHTSMultiwellinsert

systems,andMilliporeMultiscreen™CaCo96-wellplate.

TheREMSAutoSamplerisdesignedtofacilitateintegrationwithotherroboticsystems.SpeciallocatingbarsareinstalledontheREMSbaseplatformthatallowothersystemrobotstoplaceanHTStrayintoapreciselocationontheREMSbase.

TheREMSAutoSamplerwillautomaticallymeasureandrecordtissueresistancefromauser-specifiedmatrixofculturewellsonthemicroplate.Accordingtothespecifiedsequence,theroboticarmmovesovertheidentifiedwellstakingTEERmeasurements.Bymeansofax-y-zlocatingsystem,theelectrode-containingarmispositionedpreciselyandreproduciblyovereachwell.TheabilityoftheREMSAutoSamplertoreproduciblyandpreciselylocatetheelectroderesultsinhighlyreproducibleTEERmeasurements.TEERmeasurementsarestoredinthecomputerastheelectrodemovesfromonewelltothenext.TheWindows-basedsoftwareprovidesuser-friendlyfeaturestoacquire,displayandstorethetissueresistancemeasurements.

TheREMSelectrodeisverycompactandrobustindesign.Eachoftworod-shapedprobes,1.5mmindiameter,consistsofapairofelectrodes:oneelectrodeforinjectingcurrentandtheotherformeasuringthevoltage.Theuseoftwopairsofelectrodeseliminatestheerrorcausedbytheelectrode-liquidinterface.Totakeameasurement,therobotinsertsoneprobeintothecenterofthe





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WPI’sREMSTEERmeasurementsystemisalsoavailableinafullycustomizablepackagethatdoesnotincludetherobot.TheREMS-KITisdesignedtoenablemanufacturersandusersofroboticandHTSsystemstoincorporateTEERmeasurementcapabilityintotheirownautomatedprotocols.EssentiallytheREMS-KITprovidesthesameTEERmeasuringsystemastheREMSbutwithouttherobotpositioner.ControloverTEERmeasurementisaccomplishedusingtheDDEprotocol.VirtuallyanyWindows-compatibleprogramminglanguagethatusestheDDEprotocol(includingLabViewandVisualBasic)canbeused.TheREMS-KITisdesignedforusewithCorningCostarHTSTranswell-24,FalconHTSMultiwellInsertSystemandMilliporeMultiscreenTMCaCo96-wellplates.Thesystemincludesthefollowingcomponents:

●REMSTEERelectrodewith5-ftcable

●DummyTEERelectrodefortrainingrobot

●REMSTEERmeasurementelectrodeinterfaceunit

●WindowsPCIA/Ddataacquisitioncard

●InterfacesoftwareusingtheDDEprotocol

●InstructionManual

REMS KIT SPECIFICATIONSResistancerange . . . . . . . . . . . . . . . . .0to20kOhms

Timeforeachmeasurement . . . . . . . .1to2.5seconds

Minimum Configuration:Pentiumprocessor(90MHz)runningonWindows95orWindows98andhavingatleast16MbofRAM.10Mbytesoffreediskspace.

REMS KIT includes one electrode which fits either the Corning Costar 24-well microplate, the Falcon 24-well microplate, or

the Millipore Multiscreen CaCo 96-well microplate.

REMS AUTOSAMPLER SPECIFICATIONSMEMBRANERESISTANCERANGE 0to2000Ωand0to20kΩ

ACSQUAREWAVECURRENT +/-20µ[email protected]

ELECTRODEPOSITIONING ResolutioninX,YandZ:+/-1mm

ELECTRODEPERFORMANCE RepeatabilityinX,YandZ:+/-0.25mm

ELECTRODEARMSPEED X-andY-axis:250mm/sec Z-axis:247.3mm/sec

TYPICALMEASUREMENTTIME 24-WELL 1min,10sec

SCANPATTERN Choiceofanywellpatternsampling

LINEVOLTAGE Userspecified:100/120Vor220/240V

DIMENSIONS 53.5×43.7×37.1cm (213⁄32×173⁄16×145⁄8in.)

WEIGHT 24kg(52lb)

filterwellandtheotherintotheopeningslotofthe24-or96-wellplate.TheuseofACcurrenttomeasureresistanceprovidesseveraladvantagesoverDCcurrent,including:

● Absenceofoffsetvoltagesonmeasurements;

● Thereisazeronetcurrentbeingpassedthroughthemembraneandthereforeitisnotadverselyaffectedbyacurrentcharge;

● Noelectrochemicaldepositionofelectrodemetal.

TheREMSAutoSampleralsofeaturesarinseandcalibrationcheckstation.IfoccasionalrinsingoftheREMSelectrodeisrequireditmaybesenttoarinsestationbypressingtherinsestationbuttononthemenubar.Therinsestationcanalsofunctionasacalibrationcheckstationwhenfittedwithacalibrationcellcontainingasyntheticmembrane(WPI’soptionalCALICELL-HTSF).Theuseofthiscalibrationcell,whichmimicsaconfluentepithelialmembrane’sresistanceinfluid,providesaquickandeffectivetesttodeterminewhentheREMSmeasurementsystemisfullyinitializedfollowingstart-upandtocheckitsfunctionalityduringoperation.

Give your HTS system the ability to perform REMS TEER

measurements



REMS AutomatedTissueResistanceMeasuringSystemIncludes robot sampler, data acquisition board, base plate, software with Windows 95, 98, 2000, NT, and electrode for either 24well plate (Corning Costar

HTS Transwell24 or Falcon HTS Multiwell) or 96well plate (Millipore Multiscreen CaCo) — SPECIFY WHEN ORDERING.

ACCESSORIESREMS-24 ReplacementREMSSTXElectrodefor24-wellHTSPlate REMS-96 ReplacementREMSSTXElectrodeforMilliporeTM96-wellPlate CALICELL-HTSF OptionalCalibrationCellforREMSSystem

Specify type: 24- or 96-well, Millipore, BD, or Corning plate

Contact WPI for detailed information.

REMS-KIT REMS Kit for Corning Costar HTS Transwell-24 or Falcon HTS Multiwell Insert SystemREMS-KIT-M REMS Kit for Millipore MultiscreenTM CaCo 96-well

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A Microprobe Thermometer is the instrument of choice for biological and laboratory temperature measurements. These thermometers are very versatile, providing fast response, high accuracy and stability with digital display and analog signal for connection to a computer or recorder. With the wide selection of probes, the instruments can be used in almost any application.

BAT-12 This thermometer has a sealed construction making it water, dust and fume resistant. The BAT-12 has a single microprobe input and a single range with the same high accuracy as the BAT-10. Comes complete with carrying case.

The thermometers can be used with any “Type T” thermocouple. Select a temperature microprobe on the following page for your specific application.

BAT-10 This is the most versatile thermometer available. The instrument has a wide temperature range and fast response with most microprobes. The BAT-10

accuracy is NIST traceable and in each of the two temperature ranges, the accuracy is the same as the resolution. There are three microprobe inputs, 1 and 2 can be selected as separate inputs while 2 and 3 will read the differential temperature measurement between the two. The instrument has automatic warnings for low battery or faulty probes on the digital display. The linearized analog output (LOP) signal allows ease of connection to a data acquisition system or recorder.

Microprobe Thermometers Temperature Probes● Super Accuracy ● Fast Response● Analog output signal ● Multiple inputs● Differential Temperature Measurement

MICROPROBE THERMOMETER SPECIFICATIONS BAT-10 BAT-12

TeMPerATure rANge -200°C to +400ºC, 1ºC resolution -100°C to +199.9ºC, 0.1ºC resolution & reSOLuTION -100°C to +199.9ºC, 0.1ºC resolution

DIffereNTIAL TeMP. rANge -19.99°C to +19.99ºC N/A Linearization centered at 40°C 0.01°C resolution

ACCurACY 1° range 1°C ±1 least significant digit 0.1°C ± 1 digit between 0-50°C 0.1° range 0.1°C ± 1 least significant digit 0.1% ± 1 digit over full range Diff. range 0.01°C ± 1 least significant digit

rePeATABILITY ± 1 least significant digit

CALIBrATION CONfOrMITY Conforms to NIST tables follows NIST thermocouple tables within 1 digit

DISPLAY 31⁄2 Digit LCD 31⁄2 Digit LCD

INPuT SOCKeT Miniature, quick disconnect, copper-constantan Miniature, quick disconnect, copper-constantan

ANALOg OuTPuT Non-linearized set at 1.6 V, ≈ 10 mV per degree C corresponding to temperature of 401°C

POWer SuPPLY / BATTerIeS BAT-10: 4 alkaline “C” cells (life: 1000 hr) BAT-12: 9V cell BAT-10r: 4 Ni-Cad “C” cells (rechargeable unit) BAT-12r: 9V Ni-Cad with charger

SeNSOrS Three Type T thermocouple inputs One Type T thermocouple input

AMBIeNT OPerATINg rANge 15-45°C Auto-compensated to 0.1°C from 0°C to 50°C

DIMeNSIONS 21.6 x 22.9 x 8.9 cm (8.5 x 9 x 3.5 in.) 12.7 x 6.4 x 15.2 cm (5 x 2.5 x 6 in.)

WeIgHT 1.6 kg (3.5 lb), including carrying case 1 kg (2 lb), including carrying case

BAT-10

BAT-12

Stand sold separately



BAT-10R/LOP Multiple Input Type T Thermocouple Thermometer, rechargeable NiCad battenes and 110 VAC adapter (microprobes ordered separately) BAT-10R/LOP-220 Multiple Input Type T Thermocouple Thermometer, rechargeable NiCad battenes and 220 VAC adapter (microprobes ordered separately) BAT-12R Single Input Type T Thermocouple Thermometer, rechargeable NiCad battenes and 110 VAC adapter (microprobes ordered separately) BAT-12R-220 Single Input Type T Thermocouple Thermometer, rechargeable NiCad battenes and 220 VAC adapter (microprobes ordered separately)

OPTIONAL ACCESSORIESEXT-6 Probe Extension Lead, 180 cm long SBT-5 Thermocouple Switch Box, 5 probe inputs on back with numbered push button switches on the front. Connects 5 microprobes to one BAT-12R501608 Tripod Stand for BAT-12



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Microprobe Thermometers Temperature Probes

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When precise temperature measurements are required, WPI can provide you with a very accurate monitor and thermocouple microprobes. WPI monitors have both resolution and accuracy of 0.1°C in the 0-50°C range and are traceable to NIST standards, whereas, other competitive electronic thermometers have

an accuracy that is usually to 0.5°C or worse. furthermore, all our type T clinical probes are guaranteed accurate to 0.1°C, due to our stringent wire standards. These are five times more accurate than competitive probes made with regular “Special Limits” wire.

● Flexible Teflon microprobes are used for implantation in tissue, in spectrophotometer cuvettes, rectally in neonatal mice, in water baths, PCR thermal cyclers, etc.● Animal rectal temperatures during surgical procedures and pyrogen testing.● Skin temperature measurement during exercise physiology studies.



Probe Type Size Style Time Isolated Max. Lead Description Constant Temp. Length

NEEDLE MICROPROBESFast-response needle probes for instant readings in tissue, semisolids, liquids, very small specimens, powders and materials. Needle tip is sealed to ensure only stainless steel contacts specimen.MT-29/1 29 ga / 1 cm A 0.125 sec No 200°c 5 ft 29 gauge approximately 0.013-inMT-29/2 29 ga / 2 cm A 0.125 sec No 200°c 5 ft -MT-29/3 29 ga / 3 cm A 0.125 sec No 200°c 5 ft -MT-29/5 29 ga / 5 cm A 0.125 sec No 200°c 5 ft -MT-26/2 26 ga / 2 cm A 0.1 sec No 200°c 5 ft 26 gauge approximately 0.018-inMT-26/4 26 ga / 4 cm A 0.1 sec No 200°c 5 ft -MT-26/6 26 ga / 6 cm A 0.1 sec No 200°c 5 ft -MT-23/3 23 ga / 3 cm A 0.15 sec No 200°c 5 ft 23 gauge approximately 0.125-inMT-23/5 23 ga / 5 cm A 0.15 sec No 200°c 5 ft -MT-23/8 23 ga / 8 cm A 0.15 sec No 200°c 5 ft -MT-4 29 ga / 1 cm A 0.025 sec No 200°c 5 ft Similar to MT-29/1 but has a blunt tip. Good for instant skin and surface temperatures, liquidsMT-D — C 0.025 sec No 200°c 5 ft Fast response surface probe (stainless steel for locating inflammation, arteries, etc. Also for dental use.MT-29/1B 29 ga / 1 cm B 0.015 sec No 150°c 5 ft Insect Probe. Similar to MT-29/1 except sensor is welded into tip for maximum heat transfer. Other sizes made to special order.

FLEXIBLE IMPLANTABLE PROBES Designed for high accuracy on extremely small specimens such as insects, seeds, etc. Maximum insertion depth 1/8". Totally sheathed in chemical resistant Teflon. Sensor Lead DiameterIT-14 0.050" dia D 0.3 sec Yes 150°c 3 ft -IT-18 0.025" dia D 0.1 sec Yes 150°c 3 ft -IT-18EXLONG 0.025” dia. D - Yes 150°c 5 ft -

IT-21 0.016" dia D 0.08 sec Yes 150°c 1 ft -IT-23 0.009" dia E 0.005 sec Yes 150°c 3 ft For ultra fast measurements and for use on micro-size specimens. Tissue implantable with 239a. Needle (supplied). Rather fragile. Teflon coated.IT-1E 0.025" dia F 0.005 sec Yes 150°c 3 ft As IT-18 sensor except bead exposed. Combines ultra- fast reponse of IT-23 with sheath strength of IT-18.

RECTAL PROBESRET-2 - G 0.8 sec No 125°c 5 ft Rectal probe for rats typically for fast intermittent measurements. Smooth ball tip (0.125-in. dia.) with 1" long (0.59-in. dia) stainless steel shaft.RET-3 - G 0.5 sec No 125°c 5 ft Rectal probe for mice similar to RET-2 except tip diam. 0.063-in. and shaft 3/4-in. long (0.028-in. diam.)

GENERAL PURPOSEHT-1 H 0.5 sec No 400°c 5 ft “Workhorse” probe for liquids, gases, semi-solids. Plastic handle with straight stainless steel shaft. Not good for surface temperatures.HT-2 H 0.5 sec No 400°c 5 ft Like HT-1 except shaft length is 9-in.BT-1 I 0.15 sec No 240°c 5 ft Plastic handle with welded stainless steel, immersible shaft used for surface temperatures of solids, liquids, gases and semisolids. Tip is 0.02-in. diam., at right angle to probe to facilitate surface measurement.

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ATC1000 is a low noise heating system for maintaining animal body temperature during experimental procedures. The DC heater is extremely quiet in terms of electromagnetic radiation. This is essential in electrophysiological recordings which are very sensitive to electromagnetic interference.

The controller uses proportional, integral, and derivative (PID) technology in adjusting the DC voltage output. Compared with switched on/off type controllers, PID controllers provide a much more precise and stable control of temperature. The PID approach is also more immune to the variation of the experimental conditions such as change in animal size and unexpected disturbances.

The controller has dual temperature sensing inputs. One input is used to monitor and control the animal temperature. The other is used to monitor the temperature sensor in the heating pad to prevent the localized hot spots under animal. The auto tuning feature of the fuzzy-logic PID controller is easy to use and the manual setting of parameters provides the extra control ability if need. The temperature resolution of the controller is 0.1 °C. A rectal temperature probe has a 6-ft ultra-flexible shield cable and an rTD sensor. It makes convenient and precise animal temperature measurement.

Two different types of heaters are offered, a metal heating plate and a flexible silicone heating pad. The heating plate is compatible with stereotaxic systems. Its rigid flat surface fits under the u frame. The plate

● PID control for maximum temperature stability● Low noise DC heater

● Flexible pad or rigid plate option● Dual temperature sensor inputs

● Audible alarm protects the pad/element from overheating

ATC1000 SPECIFICATIONSreSOLuTION .................................................... 0.1 °CACCurACY ........................................................ ±0.3 °CSeNSOr ............................................................. rTD ∅ 2.0 mm x 25 mmMAxIMuM DC OuTPuT ................................ 27 V, 1ATeMPerATure rANge .................................. up to 45 °CPOWer ............................................................... 90-240 V, 50-60 HzDIMeNSIONS ................................................... 45 x 30 x 7 cmWeIgHT.............................................................. 11 lb (5 kg)

has a built-in temperature sensor inside. The flexible silicone heater is designed to provide the ability to fit different platform shapes and to wrap or cover the animal. It has no built-in sensor inside. All heaters are washable with water and detergent.

Animal Temperature Controller



ATC1000 Animal Temperature Controller Including rectal probe and one heating plate (specify small or medium)

OPTIONAL ACCESSORIESATC Animal Temperature Controller with rectal probe502195 Small Heating Plate with built-in rTD sensor, 10 x 15 cm502196 Medium Heating Plate with built-in rTD sensor, 15 x 25 cm502197 rectal Temperature Probe



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MPS-2Multichannel Perfusion Systemfor single ion channel and whole-cell solution exchange

MPS-2 is a programmable 8-channel perfusion system designed for single channel and whole-cell patch preparations. It offers the best combination of performance and value. The MPS-2 incorporates the same high quality solenoid valves found on similar but much more expensive systems. unlike other perfusion systems on the market, which often compromise performance to fit every possible application. The MPS-2 is the only perfusion system designed and optimized specifically for single-channel and whole-cell patch perfusion applications. The MPS-2 is therefore the best perfusion system on the market for these specific applications and is offered at a very attractive price.

The system can be controlled manually (i.e., via membrane switches on the front panel) or through a PC. Two different manual control modes are offered. One controls each channel independently and the other mode

● Manual or Programmable PC control with user-friendly GUI interface

● Fast LAFF solenoid valve

● Color-coded polyurethane tubing for easy identification

● Super low dead volume (<80 nL) micromanifold

● Economically priced

allows the user to assign a master channel that will keep the system flow when all other channels are switched off. user-friendly graphic timing software is included, and the programmed perfusion sequence can be started by computer, a patch clamp amplifier or other external trigger, or manually by the user

The perfusion fluid flows through specially designed color-coded polyurethane ribbon style tubing. The color-coding allows the user to easily trace each channel for diagnostic or set up. The ribbon style of the tubing keeps the system very neat and clean. unlike PVC based tubing, polyurethane tubing contains no plasticizer, which can caused contamination. The tubing ribbon is designed as an economical disposable item, which is often critical when cleanness is needed.

The most unique feature of the MPS-2 is its perfusion micromanifold. using the latest microfluidic techniques, the injection molded micromanifold

provides the least flow resistance and dead volume of any product on the market. The flow channel inner diameter is approximately 1.0 mm, except for the last 5 mm before the junction point. This design allows a fast flow rate without using a pressured system. The maximum flow rates are 1 and 16 microliter per second for the 15 mm long 100 µm and 200 µm ID tips, respectively. Small channels and a unique design at the merging point further reduce the chance of cross contamination. Dead volume is less than 80 nL.

MPS-2 SPECIFICATIONSChannels 8

Valve response Time 2 ms

Valve Control Serial Port, TTL, and Manual

Syringe reservoir Volume 10 mL

Manifold 8 to 1

Tip ID 200 micron and 100 micron.

Maximum flow rates (gravity fed) 100 µm ID tip, 60 µL/min. (equivalent linear speed: 12.7 cm/sec) 200 µm ID tip, 960 µL/min. (equivalent linear speed: 51 cm/sec)

Dead Volume < 80 nL excluding the single outlet tubing

inputs from 1/16” ID tubing

mounting rod connector

internal passage ID: 250 µm

14 mm OD

internal junction OD: 600 µm

output: 100 or 200 µm ID quartz/polymide tubing

Micromanifold closeup: Fluid-filled passages are shown in magenta.



MPS-2 Multichannel Perfusion System & Control Software

REPLACEMENT PARTS502109 Color-coded Polyurethane Tubing, 1/16” ID x 8 Channels, 15 ft502110 Micromanifold, 100 µm ID tip, 2 pcs/pk502125 Micromanifold, 200 µm ID tip, 2 pcs/pk

Specify line voltage and Micromanifold tip OD when ordering.

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Vibroslice uses a vibrating blade to slice tissues without the trauma produced by other methods. Live brain or other tis-sues can be cut into slices 50- to 700-microns thick. fixed tissues can be cut down to 20-micron slices (these need not be embedded or frozen). Particularly useful for improving the access for certain histological reagents (e.g., during pro-cessing for horseradish peroxidase).

The blade has a lateral displacement of about 1 mm, and its oscillating frequency may be varied between 60 and 4500 rpm. This allows you to achieve clean cuts in tissues of different mechanical consistencies.

An economical, easy-to-use precision microtome for preparing live tissue sections for physiological, pharmacological and biochemical research

Model NVSL offers a manual advance for posi-tioning the specimen holder and bath chamber. Sample positioning on Model NVSLM1 is motor-ized. Other features include independent, remov-able specimen holder and bath chamber, variable advance speed and hands-free operation via a footswitch.

NVSL & NVSLM1 SPECIFICATIONS

SeCTION THICKNeSS MINIMuM 20 µm (fixed tissue) 50 µm (fresh tissue) MAxIMuM 700 µm STeP SIZe 5 µm

CHAMBer DIMeNSIONS 21⁄8 × 31⁄4 × 11⁄4 in. (55 × 82 × 31 mm) VOLuMe 8.5 cu. in. (140 mL)

BATH ADVANCe SPeeD 0.07 to 1.6 mm/s (NVSLM1) (continuously variable) reVerSe SPeeD 4 mm/s (NVSLM1)

VIBrATION SPeeD 60 to 4500 rpm AMPLITuDe 1 mm (approx.)

POWer 95-135 V, 50/60 Hz, or 220-240 V, 50/60 Hz

SHIPPINg WeIgHT 19 lb (8.6 kg)

NVSLM1 (motorized)

NVSL (manual)

Blade speed up to 4500 rpm

Removable molded top cover for easy maintenance

Sensitive parts sealed to avoid damage from spillage

Blade angle variable through 90 degrees from horizontal to vertical

Advance speed as low as 0.07 mm/sec



NVSL Manual Vibroslice NVSLM1 Motorized Vibroslice

Specify line voltageOPTIONAL ACCESSORIESVSLM1H Spare Specimen HolderVSLM1C Spare Bath Chamber5450 replacement Belts for NVSL (2) 5451 replacement Belts for NVSLM1 (4) BLADES Blades, Single edge (100) 7600 Petlier Temperature Controller, Standard Power 7600S Peltier Temperature Controller, High Performance

See adhesives, in Lab Supplies section. See Cidex, in Microsurgery section.



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IntegraSlice™

Fully Programmable Oscillating Blade Microtome

IntegraSliceTM is controlled using sealed Liquid Crystal Display (LCD) with associated membrane keypad to set the cutting parameters of blade speed, advance speed and section thickness. There is a rotary brightness control for the cold light source. The IntegraSliceTM operation is commenced by actuating the two-stage footswitch. The first stage starts the oscillating blade and the second stage starts the advance of the blade.

INTEGRASLICE SPECIFICATIONSBLADe MOTION Arcuate with minimal vertical movement

OSCILLATION SPeeD Linear from 20-120 Hz

AMPLITuDe Of CuT Nominally 0.5 to 1.5 mm

BLADe ADVANCe SPeeD Between minus 1.00 mm to plus 3.00 mm/sec in 0.01 mm steps

BLADe reTurN SPeeD 4.0 mm/sec whilst tissue is automatically lowered from blade

VerTICAL rANge 32 mm total, in 1 µm steps or maximum speed of 1 mm/sec

SeCTIONINg rANge 1-40 mm

MAxIMuM SIZe Of SPeCIMeN 30 x 40 mm

TISSue BATH OPTIONS Standard, Autoclavable, or Temperature Controlled

CuTTINg HeAD OPTIONS Standard or autoclavable

BLADeS Stainless steel or ceramic single bevel

OBSerVATION Inspection Microscope 10x-40x with zoom and “Clearview” window

VOLTAge INPuT 115/230 Volts

rATeD freQueNCY 50-60 Hz

The unprecedented quality of slice of IntegraSliceTM

stems from its technical design features including cutting head mounted upon a parallel leaf-spring assuring blade travel along a line and arcuate path of the blade avoiding static contact of the blade with the advancing tissue. Sophisticated control allows the advance speed to be varied from minus 1.00mm/sec and up to 3.00mm/sec in steps of 0.01mm. During blade return the machine automatically lowers the tissue away from the blade and then readjusts to the original height once blade retraction has been completed. In all operations, even at the slowest rates, blade advance and blade speed is accurately maintained exactly as the user has specified due to constant feedback control. Slice thickness can be incremented in 1 micron steps and controlled to a maximum speed of 1 micron per second.

IntegraSliceTM design combines accuracy, speed and the flexibility to remove the tissue bath and mounting for transport of the tissue, for cleaning and autoclaving. integrasliceTM is supplied with a standard tissue bath and holder, which can be replaced by the autoclavable version or the temperature controlled version.

Two models available:

IntegraSlice-P — fully programmable cutting profiles of speed, distance, section thickness● Dynamically Adjustable Speed and Amplitude (DASA) - both the speed and the amplitude of

the cut can be dynamically adjusted, whilst the blade is in motion● Instrument “learns” manually controlled cycle, which can be repeated automatically● Cutting profiles can be set and stored

IntegraSlice-M — Motorized or Manual advance of the oscillating blade● A set speed motorized advance or manual advanced controlled by rotary knob dial

Once removed from the animal, tissue must be immediately cooled to lower the oxygen demand and prevent anoxia. Holding the tissue close to 4°C must continue throughout the slicing procedure. This can be done with passive cooling where a known amount of ice is used to maintain the cooled a.c.s.f. or with an electronically controlled Tissue Bath Cooler.

The control unit supplies power to the Peltier

Tissue Bath Coolers for Vibroslicethermoelectric elements in the base of the tissue bath. These act as energy transfer units so that heat is drawn off through the heat exchanger and removed by tap water flow. The unit uses P.I.D. (proportional

integral derivative) temperature control to take the bath temperature to within 0.5ºC at the point of measurement. The temperature feedback thermistor is located in the bath floor and software calculates an offset

automatically.

Immediate cooling of the tissue prevents anoxia by lowering the oxygen demand. Once removed from the animal tissue should be immediately place in cooled saline or sucrose buffer before mounting in the tissue bath. (See adhesives, pages 166-168.)

MODEL 7600SPECIFICATIONS

DISPLAY reSOLuTION: 0.1 ºCTeMP. ACCurACY: +/- 1 ºCTeMPerATure rANge: +8ºC to 0ºC

(Note that the actual temperatures achievable will be dependent upon the solutions used and local temperature conditions)

VOLTAge reQuIreMeNTS: 230V 50Hz or 115V 60Hz POWer rATINg: 60WINLeT fuSe rATINg: 2A

A

B



INTEGRASLICE-P Programmable Vibroslice for Speed, Distance and Section Thickness INTEGRASLICE-M Vibroslice with Motorized and Manual Control

Specify line voltageAccessories501634 Magnifying glass, integrally mounted

501629 Inspection Microscope, integrally mounted 10x-40x

501630 Cold Light Source, integrally mounted

501631 Ceramic Blades (5)

501632 Stainless Steel Blades (50)

7600 Controller & Standard Tissue Bath Cooler (A) 7600S Controller & Autoclavable Tissue Bath Cooler (B)

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Lab-Trax™

LAB-TRAX S-SeriesNumber of Inputs ............................. 4

ADC resolution ................................. 16 bit

Maximum Sampling rate ............... 10,000 samples / sec aggregate

Input Connectors .............................. BNC, 50 ohm (T-series: DIN-8)

Input Impedance ............................... 1M Ohm

Input range ........................................ +/- 10 V

System Noise ..................................... < 1mV (1 part in 20,000)

Digital Input/Output ......................... 4 inputs 4 outputs

Power Source ..................................... Powered via uSB (T-series: 120/240V)

Operating Current ........................... 100 mA

PC Interface ....................................... uSB 1.1

Maximum Input Voltage ................. 10 volts (100 with x10 probe)

LAB-TRAX T-SeriesNumber of inputs ............................. 4/16 differential

Input Connectors .............................. DIN-8

PC Interface ....................................... uSB 2.0 full speed

excitation Power ............................... ± 5 volt (±10%)

Input Impedance ............................... 10 g ohm

Input range ........................................ ± 10 volt

CMr

gain = 1 .................................. >80 dB (minimum)/>92 dB (typical)

gain = 10 ................................ >95 dB (minimum)/ >110 dB (typical)

gain = 100 ............................. >110 dB (minimum)/>120 dB (typical)

gain = 1K .............................. >112 dB (minimum)/ >135 dB (typical)

Noise ................................................... <10 mV (rTI)/< 2µ volt (rTI)

frequency response ...................... 100 Hz (±10%) 8-pole low pass Bessel filter

gain ................................................... 1 to 10k (set by transducer)

Power ................................................... 120/220 V AC, 60/50 Hz (switched)

Includes powerful

software!

New T-Series Lab-Trax includes built-in transducer amplifiers.

Lab-Trax hardware is available in 4-channel configurations and may include, at the user’s option, built-in amplifiers for force or pressure transducers.

S-Series (standard) devices offer 16-bit resolution over the ± 10 volt input range and exhibit less than a millivolt of noise. Data communication as well as power is managed over the uSB port, making it easy to use a laptop or a low-profile desktop computer that may not be equipped with expansion slots or serial ports.

Data is delivered to the recorder by way of very standard front-panel-mounted BNC connectors.

T-Series devices offer the same high quality recording capability but are line powered and have built-in transducer amplifiers. A wide range of force, displacement and pressure sensors can be plugged directly into the recorder using the DIN-8 connectors on the front panel.

The available Lab-Trax hardware together with WPI’s range of signal conditioners and the Data-Trax software make it the perfect choice for research systems addressing cardiovascular studies, blood pressure, cardiac

electrophysiology, hemodynamic studies of the pulmonary and coronary systems, and studies of isolated tissues in organ or tissue baths. equipment from WPI can also be used to tailor systems for epithelial studies, and many applications in neurophysiology.

for higher speed applications, see WPI-118 (next page). This 8-channel device will acquire at 100,000 samples per second at 16-bit resolution.

Gang multiple 4-channel systems in the same copy of Data-TraxMultiple Lab-Trax units can be “ganged” using the Data-Trax software (version 2.0). When pluged into different uSB ports of a computer, the Lab-Trax inputs are collected by Data-Trax and displayed just as channels from a single source. for example, two 4-channel units can be ganged to produce an 8-channel acquisition system. As many as eight units can be ganged to collect as many as 32 channels at once. The Views feature in Data-Trax makes it easy to keep track of all the channels. And, of course, all analytical features are incorporated — including scripting and Matlab compatibility.

Enjoy the easiest, most powerful data acquisition with



LAB-TRAX-4 4-Channel Data Acquisition System LAB-TRAX-424T 4-Channel Data Acq Sys w/ Transducer Amps

OPTIONAL ACCESSORIES2851 BNC-to-BNC Cable, M-M, 6 ft3491 DIN8 extension Cable, M-f, 5 ft



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WPI-118 is a fast, high-resolution data acquisition system suitable for most data recording studies in the research laboratory. It offers eight analog input channels, eight digital outputs, four digital inputs and two DACs. The hardware connects to PC-compatible computers via the popular uSB interface, so setup is plug-and-play easy. The small physical size of the WPI-118 requires very little bench space.

Key Hardware FeaturesAnalog Outputs — WPI-118 offers two +/- 10V Analog Outputs (DACs). each is independently programmable and can be synchronized to work together. Basic stimulus parameters for each DAC, such as pulse width, frequency and amplitude, can be changed on-the-fly using handy controls located in the Data-Trax software toolbar. Standard protocols include pulse, train, and step waveforms. each standard protocol allows the quiescent state to be a holding voltage, thereby making it ideal for voltage clamping applications.

Resolution and Noise — WPI-118 employs a 16-bit A/D converter to sample data over its 10-Volt input range. Typical noise on any input is less than 1 millivolt. This allows the recording of signals from 10 mV to 10 V without the need for additional external gain.

Digital Input/Output — eight digital output lines are available. The digital output connectors are industry standard BNC, eliminating the need for custom cables. Programming the output lines is point-and-click easy as well, no complicated scripting language is required. four digital input lines are also provided, with one configured as an external trigger.

Speed — The WPI-118 is a fast recorder. At top speed it can collect 100,000, 16-bit samples per second on a single channel. With eight channels enabled, it can collect data at 10,000 samples per second on each of the open channels.

USB Connection — Connection to the computer is accomplished over the popular uSB port. This eliminates the need to install special interface cards and even makes the WPI-118 compatible with notebook computers. The WPI-118 has a small footprint, so it fits in even the tightest rack spaces.

System Requirements — WPI-118 requires a minimum Pentium II-level computer running Windows 98, Me, 2000 or xP with at least 64 megabytes of rAM, and at least 10 megabytes of free space on the hard drive. Internet connection is required for free automatic upgrades.

See complete specifications at www.wpiinc.com

High Performance Digital Data Recorder

● 100 KHz max. sample rate● Low noise● Two independent DAC

stimulator outputs● 16 programmable I/O lines● USB interface

WPI-118 SPECIFICATIONS

Input

Number of Inputs 8 single-ended BNC

Input Impedance 1 MOhm

Input range ±10V

Noise 1 LSB (1mV typical)

gain x1

Digital Input Lines 8 (4 dedicated and 4 programmable)

Output

Number of DACs 2

DAC resolution 12 Bit

DAC Speed 100k s/sec, independent of sample speed

DAC Output range ±10V

DAC Modes Pulse, Train, Step, DC

DAC Performance

Pulse Width 0.01ms-6,500 ms (pulse mode)

frequency 0.2Hz-50kHz (pulse Mode)

Digital Output

Lines 8 (4 dedicated and 4 programmable)

Digital Output Connector BNC

A/D Converter Sample Speed 1 sample/second - 100k samples /second

resolution 16 bit

Interface uSB

enclosure Aluminum (Ce compliant)

Power 120V/220V, 50/60Hz

Software Data-Trax

Trigger Modes external Trigger, Threshold Trigger from Data, user Trigger

Display real time, user definable screen time independent of sample rate, user definable units, AutoScale, full scale or user defined scale

upgrades Software and firmware upgrades are free, and are automatically delivered to registered users via the Web. Data-Trax software may be freely distributed.



WPI-118 High Performance Digital Data recorder

OPTIONAL ACCESSORIES2851 BNC-to-BNC Cable, M-M, 6 ft3491 DIN8 extension Cable, M-f, 5 ft

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The acquisition and data display in Data-Trax is easy and straight forward. It supports real units and a time-based display that is not coupled to sample rate. You can display up to 1,000,000 data points per channel on

the screen. Scrolling, zoom-in and zoom-out tools, together with a searchable list of user interventions, make finding important areas of data easy. And, of course, you can print or export anything that you see.

Powerful software included with Lab-Trax™

The Main Display

Data recorded with Data-Trax is presented as distance-per-unit of time, e.g., cm/sec, exactly as it would be on a traditional paper chart recorder. The x-axis display is compressed or expanded using point and click icons in the Data-Trax toolbar. Data-Trax’s display can smoothly scroll data at any speed. The display scroll rate is not determined by sampling rate, so you have full control over resolution and display. This is particularly useful when you want to compress high-resolution data on the time axis to create a data overview. Currently the software can display 100,000 points per channel online and one million points per channel offline so you can see quite a bit of data at a glance.

Data-Trax’s Main Window currently supports functions that can show you the real-time rate, integral or any of 16 online analyses. each of these is set up from a single click in the Main Window. Data-Trax also has an “Analysis Window” where students can make more in-depth measurements and build lab reports. There are 31 different kinds of measurements supported in this window.

Main Display

Record and analyze your data with

WPI’s coordinated system of hardware and software allows life science researchers to record and analyze a range of data types with ease and simplicity. Low profile, low cost, high flexibility and utility make Lab-Trax devices the perfect choice for applications involving tissue and organ bath recordings, epithelial voltage clamp studies and a host of cardiovascular applications — just to mention a few.





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from an easy-to-use sequence builder that can include both the digital outputs and the analog outputs. Sequences are remembered by Data-Trax and can be called manually or automatically.

Lab Settings

Settings or templates for the various lab protocols are stored in the program’s settings menu. each setting can include a Lab procedure and Journal format, as well as the program acquisition and analysis settings. This makes changing program settings to accommodate different experiments point-and-click easy.

Free Upgrades and Built-in Site License

Data-Trax software upgrades are free. Better yet, Data-Trax may be freely distributed and copied to other lab computers.

That means that researchers can record data in the lab and work on analysis and lab reports at their leisure in different locations.

Data-Trax is provided on an open license so you can record data in the lab and analyze it in your office without buying multiple copies of the software..

AutoScale

A single button allows you to find the data on a channel, wherever it is, center it and expand it to fill the available channel space. AutoScale used with high-resolution recorders ensures that you will never again lose data because they are “out of range or off scale“.

Cursors

Most measurements students make on recorded data involve a distance in time or a difference in amplitude. for this reason, Data-Trax places two easy-to-move cursors in

each display of data. Data-Trax displays the difference in time and the difference in voltage (or calibrated units) between the cursors. Cursors can be moved with the mouse, or for precise placement, with the arrow keys on the keyboard.

Real World Units

Data-Trax allows you to calibrate the displayed data in any unit that you choose. reading your data in mmHg or grams or micro amps takes the guesswork out of analysis.

Stimulator Window

Input/Output Window

Real-Time Marks

researchers can insert “Text Marks” from the keyboard into their data to indicate when events like drug delivery or stimulus points are occuring. Marks are easy to search and the ”go To Mark” feature will take you to a selected event from anywhere in the data. for reporting,

researchers can position the text in a given mark over the data it applies to, just like writing on chart paper.

Twin DACs (WPI-118 only)

Data-Trax allows you to program two independent analog outputs. They can be used as independent stimulator outputs, or as linear controls for pumps or other hardware that accept an analog voltage as control. Built-in wave protocols include pulse, step, pulse train and DC. The two outputs can be combined to produce more complex protocols.

Digital Inputs

up to eight digital inputs can be configured from Data-Trax. raw data, count frequency, and open times can be displayed in real time.

Digital Output

up to eight digital outputs can be configured from the Data-Trax software. Digital outputs can be used to control valves or relays in the experimental rig, or they can be configured in parallel to control serial devices.

Output sequences of events are created

Marks Window

Units Window



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Analyses of the First Kind — Wave-to-Wave Transformseach of these functions takes all of the data in a channel and applies a transform to the data. The transform converts the entire waveform described by the data points into a completely new wave that is displayed on an adjacent channel. examples of these functions are the 1st derivative and the integral. Currently there are 27 functions of this type included in the Data-Trax program. They are called from the right-click menu of a channel and can be used on-line or off-line. When

used on-line, they can operate at the top acquisition rate of the program, 100,000 samples per second.

Analyses of the first Kind that are supported, include: rate, frequency, Period, Minimum, Maximum, Mean, rMS, regular Integral, Absolute Value of Integral, first Derivative, Second Derivative, user Definable Channel Math function, Digital filter, Power, gain Telegraph (25 amplifiers supported), Calculated Cardiogram Leads (4), Cardiac Angle, HrV Power (3 bands), Spirometry Volume.

Today’s analog to digital converters make acquiring data very easy. The challenge to all users is to extract meaning from the mountains of recorded data in order to generate conclusions, reports, and Nobel Prizes. This process is called analysis.

Built into the Data-Trax data recording software is a powerful array of data analysis tools. The variety of tools available strike a balance between the

straightforward, general operations that everyone uses and the vertical, complex routines that only YOU use. So, Data-Trax is a powerful analytical tool that can go to work on data right away, or be customized to execute very specific, complex analysis routines.

Analysis in Data-Trax is divided into five types or kinds, each addressing specific analytical requirements.

Not just data acquisition, but powerful analysis too!

Analyses of the Second Kind — Reduced Datafunctions in this class take a user-selected group of data points and “digests” them to a single value. These values are then saved to an on-board Journal where they can be saved or exported to other programs. There are currently 31 functions of this type available within the Data-Trax software.

Analyses of the Second Kind that are supported, include: T1,Y1,T2,Y2,T2-T1 Y2-Y1,





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above or below a specified level, or data at a particular time. The program can look for values at a mark containing a particular string, or any combination of these (e.g. look for a value 10 seconds after a comment containing the string “Drug”). The find command can be made to automatically drop the values that it finds into a tab-delimited table that can later be opened in excel, Matlab, or other analysis programs.

SPeCIfIC events are detected using algorithms to find the point or region of interest. The Left Ventricular end Diastolic Pressure (LVeDP) and finding r waves or P waves in an eCg are good examples. Detection of these data points requires specific software that resides in the Data-Trax program, accessible from the Auto Mark menu. AutoMark allows the user to choose a detection algorithm and a data set. When the detection algorithm is completed, the software marks the record with comments at the appropriate places. Commenting or marking in this context accomplishes two things; it identifies the data points that need to be operated on, and it also provides the user with visual cues as to whether or not the software did its job in detecting the points that it was to find. Data-Trax can be directed to export the found data as text or load it into a user-defined script. The Auto Mark can currently detect LVeDP, the QrS components of the eCg dichrotic notch, maximal response for organ or tissue bath studies, and spikes.

Analyses of the fourth Kind include: Auto find: Min, Max, Threshold, Mark with Specific String, Time Auto Mark: r waves, LVeDP, Dichrotic Notch, Maximal response, Spike Detection, eCg Analysis.

Analyses of the Fifth Kind — ScriptingIt is impossible to include all of the analyses and plotting capabilities required for biological research in a single program. We have provided what we believe to be useful tools for the most common kinds of analyses. In those circumstances where the built-in analysis tools fall short of what is required, we have provided a scripting function. A script

is a program written in any of the languages supported by Data-Trax. Scripts appear as items in the Data-Trax Scripting menu. When elected, the script takes a user selected range of recorded data and executes the script on the data.

Scripting languages supported: DADiSP, Python, MatLab, LabView, C++.

Maximum, Minimum, Maximum-Minimum, Mean, Mark, Count, units, Standard Deviation, rMS, Maximum dV/dt, Minimum dV/dt, Mean dV/dt, dV1/dt, dV2/dt, Area, Integral, Absolute Value of Integral, Average of Maximums, Average of Minimums, Average of Means, Average Period, Count, Average of Maximum-Minimum

Analyses of the Third Kind — Redisplayed Data

redisplayed data involves the plotting of recorded data in an “Other than Y, T” format. examples include the xY plot or the fast fourier Transform (ffT). Currently, Data-Trax 1.8 supports xY and ffT. Measurements of areas, segment lengths and slopes, as well as data point values are available as “Second Kind” analyses from these windows.

Analyses of the Fourth Kind — Detected Data ConditionsData-Trax software can be used to identify regions of Interest (rOI) in recorded data. Measurements can be extracted from these detected data points or areas, and then, be sent to the Journal where the

measurements can be included in a report. The measurements can also be exported in a variety of formats.

Data-Trax can detect events in two categories: general and specific. geNerAL events are the ones located in the find dialog. using stock parameters, Data-Trax can identify Minimums and Maximums, data

Record and Automark the “R” wave.

Analysis of the Third Kind (FFT)

Capture “R” wave values and pass to the Marks Window prior to export.

Easily export “R” values to Excel or other ana-lytical program for further analysis.



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FluoroDish™

Cover-glass bottom for observing and growing cells in imaging related research

WPI’s FluoroDish™ tissue culture dishes are now available in a larger range of sizes, color and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range.

Conventional plastic dishes and chambers limit the utility of the inverted scope for many applications because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17 mm thick) optical quality glass bottom, allowing the use of a much shorter objective working distance, larger numerical aperture (NA), and a higher magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in Image Analysis. The glass bottom dish with black colored wall is particularly suitable for fluorescence imaging which requires lower

background scattering of light and reduced intrinsic “auto” fluorescence, which occurs when using standard plastic bottom dishes. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Three different sizes of FluoroDish are offered, one type of 50 mm diameter dish and two types of 35 mm diameter dishes. An inner well is created within the dish by the glass bottom and the tissue culture grade plastic which forms the sides of the dish. All WPI dishes have the advantages of low toxicity and good UV transmission bottom glass. They are individually packed and gamma sterilized.

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window (FD35) or ø10 mm glass window (FD3510). Most heaters and perfusion adapters designed for the Corning 35 mm dish will also fit this dish. The 23.5 mm glass window dish is available uncoated, poly-D-lysine-coated, or collagen-coated with clear wall and uncoated with black wall. Certain types of cell lines (e.g., PC3 and HEK) adhere well

to the uncoated glass bottom dish. The poly-D-lysine coating has been reported to improve the adhesion of neuron cells, and type I rat tail collagen has been reported to improve the adhesion of muscle cells. The users can also apply to the uncoated dish any special coating that is best for their cell line.

The 10 mm glass window dish (FD3510) is available with clear wall and black wall. It has low sidewall for easy microelectrode access and low solution volume. The low microelectrode access angle is the lowest among all of 35 mm dishes on the market (very close that of a 50 mm dish). The dish needs only 100 ~ 200 mL to cover the bottom well, an important feature when using expensive drugs and chemicals.

The 50 mm dish (FD50) has a large growth area (35 mm well diameter), a low access angle for microelectrodes, and grips for easy handling. They are available with clear wall and black wall.

l Optical quality glass bottom for better imaging quality (RI=1.525)

l Low sample volume for expensive chemicals

l Lowest access angle for micropipette

l Available in black wall for low background fluorescent measurement

l Low toxicity adhesive for embryo research

l Choose from poly-D-lysine or collagen coated, or uncoated

H

OD

Glass Ø

ID

AccessAngle

h

Part Number ID (mm OD (mm) Glass Ø (mm) height (inside) Height (outside) Access Angle FD35 33 35.5 23.5 7.8 9 29° FD3510 10 35.5 10 1.5 4.65 17° FD5040 47.5 49.82 35 7.25 7.4 17°



FD35-100 FluoroDish Sterile Culture Dish, clear wall, 35 mm, 23 mm well, box of 100FD35B-100 FluoroDish Sterile Culture Dish, black wall, 35 mm, 23 mm well, box of 100FD35COL-100 FluoroDish Sterile Culture Dish, Collagen Coated, clear wall, 35 mm, 23 mm well, box of 100FD35PDL-100 FluoroDish Sterile Culture Dish, Poly-D-Lysine Coated, clear wall, 35 mm, 23 mm well, box of 100FD3510-100 FluoroDish Sterile Culture Dish, clear wall, 35 mm, 10 mm well, low sidewall, box of 100FD3510B-100 FluoroDish Sterile Culture Dish, black wall, 35 mm, 10 mm well, low sidewall, box of 100FD5040-100 FluoroDish Sterile Culture Dish, clear wall, 50 mm, 35 mm well, box of 100FD5040B-100 FluoroDish Sterile Culture Dish, black wall, 50 mm, 35 mm well, box of 100



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Cover SlipsThese cover slips made of german glass

can be used for growing and culturing cells that normally have poor adhesion to plastic surfaces. They are small enough to be placed in the micro plate or other cell

culture devices. The 5 mm size will fit inside the 96-well culture plate and leave enough

room to pick it up from the bottom of the well with forceps. The 8 mm size fits inside

the 24-well plates.

ABM SPECIFICATIONSfreQueNCY rANge Audible

INPuT reSISTANCe 1012 ΩMAxIMuM APPLIeD INPuT VOLTAge ±10 V

POWer Nine-volt alkaline battery, supplied

PITCH (VOLTAge SeNSITIVITY) 1 octave per 100 mV @ 1 kHz

DIMeNSIONS 3 × 2.75 × 2 inches (8 × 7 × 4 cm)


Audible Baseline Monitor

A complete, ready-to-use instrumentLow-Pass FilterLPF30 SPECIFICATIONS

INPuT IMPeDANCe 1 MΩgAIN ×1, ×10

BANDWIDTH fILTer 100 Hz to 30 kHz (2 pole r/C)

SHArPNeSS 40 dB/decade

eQuIVALeNT SHOrTeD INPuT NOISe AT x10 gAIN 100 Hz Bandwidth 10 µVpp 30 kHz Bandwidth 30 µVpp

OuTPuT VOLTAge rANge ±8 V

MAx. INPuT VOLTAge ±10 V

OuTPuT reSISTANCe 100 ΩLOW BATTerY INDICATOr LeD lights when approximately 60 hours of battery life remain.

POWer 2 nine-volt alkaline batteries, supplied

BATTerY LIfe About 400 hours

DIMeNSIONS 5 × 3 × 2 inches (13 × 8 × 5 cm)


upper frequency band limit can be smoothly varied between 100 Hz and 30-kHz using a single knob. WPI’s new low-pass filter is small, consumes little power, and can be located almost anywhere in your workspace. features switch-selectable gain of ×1 or ×10 and low noise. An ideal tool for filtering an analog signal before digital conversion by data acquisition systems.



Order Number Diameter Thickness Quantity Price

502040 5 mm #1.5 (0.16 - 0.19 mm) 100

502041 8 mm #1.5 (0.16 - 0.19 mm) 100

The ABM, a battery-operated voltage-con-trolled oscillator, lets you monitor potential audibly. ABM is particularly useful because it allows the user to estimate voltage levels when using a microscope or engaged in other tasks which do not allow viewing an oscillo-scope or recorder. An audible tone is generat-ed with increasing pitch as the input voltage becomes increasingly positive.

ABM Audible Baseline Monitor

LPF30 Lo-Pass filter

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Model 900A is designed to measure hydrostatic pressure in small vessels and cells. Pressure ranges of -200 to +400 mm Hg can be measured with stability and accuracy. The system’s sensing element is an electrolyte-filled glass microelectrode with a tip diameter range of 2 to 5 microns.

Pressures of electrolyte solutions are measured by maintaining a salt concentration gradient at the tip of the sensing electrode in dynamic equilibrium by applying an equal air pressure inside the micro-electrode. The pressure reading appears on the front panel display and via the BNC recorder output.

Because the piezoelectric pressure controller uses external pressure and vacuum sources, pressures lower than -200 to greater than +400 mm Hg can be quickly and accurately measured at the micro-electrode tip.

The open pressure chamber is almost immune to vibrations and movements and, unless they are extremely large, the open system is unaffected by leaks. The pressure controller is contained in a small, lightweight enclosure that can easily be mounted near the micropipette to help reduce dead space. It includes an amplifier, a piezoelectric valve and a pressure transducer. The user supplies fluid-filled microelectrodes, +500 mm Hg pressure source and a -300 mm Hg vacuum source.

Measuring electric potential and pressure simulta-neously lets you use potential recording as an addi-tional cue for locating the electrode where visibility is limited, or correlate pressure and potential when this is meaningful.

The unique “Set Pressure” mode lets you preset the internal pressure of the microelectrode — select

a positive pressure for flushing the tip, or a negative pressure for pulling solution into the tip. By discon-necting the microelectrode holder and attaching the tubing to a manometer, you can check the calibra-tion against a standard.

A built-in alarm sounds to indicate maximum pres-sure. The alarm also sounds when the tip is blocked or electrical continuity is broken (e.g., the micro-electrode comes out of the solution, too little filling solution to cover the Ag/AgCl pellet, disconnected ground reference, etc.).

The piezoelectric pressure controller regulates inter-nal pipette pressure by controlling air flow into and out of a small pressure chamber. A vacuum source is connected on the outlet side of the chamber, and a piezoelectric valve meters air entering the pressur-ized chamber. The residual volume of the pressure chamber includes the micropipette, the connecting

tubing and the pressure transducer on the outlet side of the piezoelectric valve. The 900A accurately controls and adjusts the pressure in the chamber to match pressures applied externally to the microelec-trode tip.

The response time of the piezoelectric valve is 0.5 ms from fully closed to fully open. Overall sys-tem response time depends largely on the amount of residual volume in the tubing. When this volume is small, the system responds very rapidly (typically 10 milliseconds).

The lightweight pressure controller pod may be mounted close to the microelectrode using small-bore tubing, to minimize system dead space.

Microelectrode holders MEH6RF and MEH6SF for 1.0 mm O.D. capillary glass included. (1.2, 1.5 and 2.0 mm also available — please specify when ordering.)

Model 900A Micropressure System

● Simultaneously measures electric potential and pressure● Preset internal microelectrode pressure

● Air-filled system — no debubbling

Measure hydrostatic pressure in small vessels and oocytes



900A Micropressure SystemSystem price includes a one-day technical training session at WPI in Sarasota, Florida.


OPTIONAL ACCESSORIES900AP replacement ProbeCAL900A Pressure Calibration Chamber3491 Probe extension Cable2933 rack Mount Kit5332 replacement Liquid TrapMEH6RF Micropipette Holder (1.0, 1.2, 1.5 or 2.0 mm — Specify O.D.) MEH6SF Micropipette Holder (1.0, 1.2, 1.5 or 2.0 mm — Specify O.D.) TIPTW900A Prepulled Micropipette for 900A (1 mm thin-wall, 2 µTip) (pkg of 10) LV140-Y Vacuum Pump (120V) 900APP replacement Pressure PodPM015D Pressure Manometer (15 psi)



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Sensor Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Hypodermic Sheath . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Free Radical DetectionHydrogen Peroxide & Oxygen Sensor Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

ISO-HPO-2, ISO-HPO-100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

ATP Sensors WSA2001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

NEW APOLLO 1000 One-channel Free Radical Analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

APOLLO 4000 Free Radical Analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Multi-Port Measurement Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

SuperLuminol Superoxide/Reactive Oxygen Species Determination Kit . . . . . . . . . . . . . . . . . . 90

PeroxiLuminol Hydrogen Peroxide Determination Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

GSH Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Nitric Oxide DetectionNitric Oxide Sensor Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

ISO-NOP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

ISO-NOPNM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

ISO-NOPF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

ISO-NOPMC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

ISO-NOP007, ISO-NOP30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

ISO-NOP30-L, ISO-NOP70-L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

NSA NO Sensor Pre-Polarizer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

JUV ISO-NOP Rejuvenator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

SNAP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

GSNO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Nitralyzer-II Nitrate to Nitrite Reduction Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

GRNK Griess Reaction Nitrite Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Neurotransmitter DetectionMicro C Carbon Fiber Potentiostat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

Carbon Fiber Microelectrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Oxygen DetectionOxyMini & OxyMicro Fiber Optic Oxygen Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

ISO2 Dissolved Oxygen Meter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

pH Meters and ElectrodespHOptica™ Fiber Optic pH System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

Beetrode™ Micro pH Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

pH Meters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

CALBUF Calcium Calibration Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Dri-Ref™ Reference Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Kwik-Tip™ Ion-selective Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Liquid Ion Exchangers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Biosensing


World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc .com • Internet: www.wpiinc.com

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Sensors



MacrO SENSOrS

SpEcIES Nitric OxideHydrogen peroxide

Oxygen Hydrogen Sulfide

Order Number ISO-NOp ISO-HpO-2 ISO-OXY-2 ISO-H2S-2Price

Available Diameters 2 mm 2 mm 2 mm 2 mm

Response Time < 5 sec < 5 sec < 10 sec < 5 sec

Detection Limit/Range 1 nM < 100nM to 100mM 1%-100% < 5nM

Sensitivity 2 pA/nM 0.02 pA/nM N/A 2 pA/nM

Drift None 0.1pA/min < 1%/min

Temperature Dependent Yes Yes Yes Yes

Physiological Interference None None None None

Replacement Sleeves (pkg of 4) #5436 #600012 #5378 #600016

Filling Solution #7325 #100042 #7326 #100084

Start-up Kit #5435 #600011 #5377 #600015

MINI SENSOrS

SpEcIES Nitric OxideHydrogen peroxide

Oxygen aTp

Order Number ISO-NOpF ISO-HpO-100 ISO-OXY-100 WSa2001 (starter kit)

Price (pkg of 2) (pkg of 3) (pkg of 2) (pkg of 5 + 5 null)

Available Diameters 100 & 200 µm 100 µm 100 µm 50 µm

Available Length 2 mm, 4 mm 2 mm, 4 mm disk 0.5 mm, 2 mm

Response Time < 5 sec < 5 sec < 5 sec 10-90% rise time 10 sec

Detection Limit/Range 0.2 nM < 10 nM-1mM 0.1%-100% 0.5 µM to 50 µM

Sensitivity 10pA/nM 1 pA/nM N/A 0.5 nA per µM

Drift none 1.0 pA/min < 1%/min none

Temperature Dependent slight slight slight slight

Physiological Interference Yes Yes Yes

Microsensor Cable #15810 #15810 #15810

Available with Hypodermic Sheath ISO-NOpFH ISO-HpO-100H ISO-OXY-100H

Price (pkg of 2) (pkg of 3) (pkg of 2)

Available as “L”-shaped for tissue bath ISO-NOp70L ISO-HpO-100L

Price (pkg of 2) (pkg of 3)

MIcrO SENSOrS for NO

Order Number ISO-NOp30 ISO-NOp007 ISO-NOpNMPrice (pkg of 3) (pkg of 3) (pkg of 3)

Available Diameters 30 µm 7 µm 100 nm

Available Length 0.5 mm, 2 mm 0.1mm, 2 mm 0.2 mm

Response Time < 3 sec < 3 sec < 3 sec

Detection Limit/Range 1nM 0.5 nM 0.5 nM

Sensitivity 1~4pA/nM 1~4pA/nM 0.5 pA/nM

Drift none none none

Temperature Dependent yes yes some

Physiological Interference none none none

Cable #15810 #15810 #15810



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Nitric Oxide Hydrogen Peroxide

Hydrogen Sulfide Oxygen ATP

Cell Culture ISO-NOP ISO-HPO-2 ISO-H2S-2 ISO-OXY-2 WSA2001

Cell Suspensions ISO-NOP ISO-HPO-2 ISO-OXY-2

Mitochondria ISO-OXY-2

Subcutaneous ISO-NOPF

Brain Slice

ISO-NOPF3ISO-NOP30ISO-NOP007

ISO-HPO-100 ISO-OXY-100 WSA2001

Arteries ISO-NOPFISO-NOP30

WSA2001

Microvessels ISO-NOPFISO-NOP30

ISO-HPO-100 ISO-OXY-100

Kidney Tubules ISO-NOP007

Tissue ISO-HPO-100 ISO-OXY-100 WSA2001

Single Cell ISO-NOPNM

Tissue Bath ISO-NOP30-LISO-NOP70-L

ISO-HPO-100L ISO-OXY-100L WSA2001

in vivo WSA2001

*Hypodermic Sheath

Any of the 100 micron electrochemical mini-sensors can be purchased (at extra charge) with a hypodermic

sheath . This implementation uses a 24-ga . hypodermic needle to mechanically shield

the sensor from damage . Its use allows effortless insertion into blood vessels,

muscle and tissue of all kinds without breakage .

Specify "NEEDLE" when ordering.

Sensors applications

Micrometer

connect to Microsensor cable #15810


10 mm

3 mmcarbon fiber

FORT-100 Transducer

Bath≥ 10 mL

Organ & Tissue Bath Studies

The L-shaped 100 micron electrochemical mini sensors were designed specifically for use in tissue bath studies and similar applications . The shape of the sensor has been engineered to facilitate placement of the electrode within

the lumen of the tissue vessel under study . The ISO-NOP70-L is similar in construction to the ISO-NOP30 but with the advantage of having a flexible tip

(70 µm diameter) .



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ISO-NOP – The original nitric oxide probe – ideal for cell cultures, cell suspensions and many other applications

The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve . The tip of the sleeve is covered with a NO-selective membrane . Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325) .

The ISO-NOP is an excellent general purpose NO sensor and is included with each NOMK2 .

NO–3 and NO–

2 Detection

A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for rapid accurate determination of nitrite (NO–

2) and nitrate (NO–3) concentration in

samples . Using this method a detection limit for NO–

2 or NO–3 as low as 1 nM is routinely

possible .

ISO-NOPNM – The world’s smallest nitric oxide NanoSensor, designed for measurement of NO at the cellular level.

The ISO-NOPNM NanoSensor has a tip diameter of just 100 nm (0 .1 µm) and a detection limit for NO of less than 0 .5 nM

— making it indisputably the smallest and most sensitive NO sensor in the world!

The ISO-NOPNM is based on a novel design in which an electrochemically “activated” composite graphite nanofibre is used as the NO-sensing element . The surface of the NanoSensor is then modified using a unique multi-layered NO-selective membrane . Figure at right illustrates the response of the ISO-NOPNM following successive additions of nanomolar concentrations of NO . The ultra-low noise of the ISO-NOPNM (0 .5 pA) enables a detection limit of just 0 .5 nM NO . The response time of ISO-NOPNM is less than 3 seconds .

Schematic drawing of the new integrated NO nanosensor. (US Patent Pending)

Amperometric response of the NO nanosensor (ISO-NOPNM) to the successive additions of 2nM, 4 nM, 8 nM NO into 0.1 M PBS (pH=7.4).

Time / S

Cu

rren

t /

pA

y = 0.5968x + 6.4967R2 = 0.9973

0

200

400

600

800

0 300 600 900 1200

Concentration / nM

Cu

rren

t /

pA

Nitric Oxide Sensor GuideAll WPI NO sensors are 100% compatible with the original ISO-NO, the more recent ISO-NO Mark II (NOMK2)

and the new APOLLO 4000 Free Radical Analyzer.

APPLICATIONS invivo CellCultures, TissueBath TissueBath Microvessels Microvessels SingleCell NO-

2,NO-3,

SENSOR ISO-NOPF ISO-NOP ISO-NOP30L ISO-NOP70L ISO-NOP30 ISO-NOP007 ISO-NOPNM

SENSORDIAMETER 100&200µm 2mm 30micron 70micron 30micron 7micron 100nmRESPONSETIME(withNOMK2) <5sec <5sec <3sec <3sec <3sec <3sec <3secLOWESTDETECTIONLIMIT 0.2nM 1nM 1nM 1nM 1nM 0.5nM 0.5nMTEMPERATURESENSITIVITY some yes yes yes yes yes someDRIFT none none none none none none noneSENSITIVITY 10pA/nM 2pA/nM 1.4pA/nM 1.4pA/nM 1.4pA/nM 1pA/nM 0.5pA/nMPHYSIOLOGICALINTERFERENCE none none none none none none none

Abdominal X-ray showing the appratus consisting of two customized ISO-NOP nitric

oxide probes, 4-channel pH catheter, and Teflon nasogastric tube. (Courtesy Prof. K.E.L.

McColl, University Department of Medicine and Therapeutics, Western Infirmary, Glasgow,

Scotland.) Iijima, K., et al. Gastroenterology 2002: 122: 1248-1257.

Selectivity of WPI’s NO sensorsThe ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment . Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species .

WPI’s unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO whilst eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more .

Ag/AgCl WPIMembrane

100 nm

≈150 µm

SensingElement


UK: Tel: 01438-880025 • Fax: 01438-880026 • wpiuk@wpi-europe .com Germany: Tel: 030-6188845 • Fax: 030-6188670 • wpide@wpi-europe .com

ISO-NOPNM 100nmNanoSensor,pkgof3(requirescable#15810)15810 Microsensor Adapter Cable

ISO-NOP Replacement 2 mm shielded sensor and cable5435 ISONOP Startup Kit (recommended with first purchase) 5436 Replacement Sleeve Kit for 2 mm sensor, pkg of 47325 ISO-NO Electrolyte (10 mL) 7521 ISO-NO Electrolyte, CO2-insensitive (10 mL) 5399 T-Adapter Kit (pkg of 3) for ISO-NOP



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ISO-NOP007ISO-NOP30 – 7 and 30 micron sensors with exceptional performance — ideal for tissues and microvessels

The ISO-NOP007 and ISO-NOP30 have recently been improved in design and performance . The ISO-NOP007 has a tip diameter of just 7 microns and a length of 2 mm . The ISO-NOP30 has a tip diameter of 30 microns and is available in two different tip lengths (i.e., ISO-NOP3020 has tip length of 2 mm, ISO-NO3005 has tip length of 0 .5 mm) . The response of the ISO-NOP007 and ISO-NOP30 is linear over a wide dynamic concentration range of NO . The design of both electrodes is based on a single carbon fiber coated with WPI’s NO-selective membrane . A detection limit of approximately 1 nM NO makes these electrodes ideal for use in tissues and microvessels .

Cu

rren

t (p

A)

Time (s)

Cu

rren

t /

pA

Concentration / nM

1600

1200

800

400

3000 600 900 1200

0

y = 1.3583x + 16.15R2 = 0.9985

30

0 p

A

20 sec

The response of a 7 µm NO sensor (ISO-NOP007) to successive additions of NO (100 nM). Inset shows the linearity of the resulting calibration plot.

Simultaneous measurement of force (top trace) and changes of NO concentration (lower trace) in (A) the rat superior mesenteric artery relaxed with ACh and (B) a small human artery relaxed with ACh and SNAP. In this artery oxyhaemoglobin(oxyHb) partly reversed the increase in NO concentration, with only a small change in force. [U. Simonsen, et al ., J . Physiol ., 1999, 516: 271-282.]

Schematic drawing of ISO-NOP007 and ISO-NOP30.

ISO-NOPF – unique flexible NO sensor! Designed for arteries, microvessels, in vivo applications, and similar applications.

ISO-NOPF electrodes are the newest addition to WPI’s nitric oxide sensor family and are available in 100 µm and 200 µm diameters . Utlilzing the latest advances in nano-technology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors . The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode . The surface of the sensor is then coated with a unique multi-layered NO-selective membrane .

Time (s)

Cu

rren

t (p

A)

2 nM

4 nM

8 nM10 sec

50

pA

1 nM

Response of ISO-NOPF to NO.Schematic drawing of ISO-NOPF.

Ag/AgCl

100 µmor200 µm

5 mm

2 mm

flexible

Sensor Body60 mm x < 2 mm diam.

Ag/AgCl

7 µmor30 µm

2 mm

Sensor Body60 mm x < 2 mm diam.

2 mmor

0.5 mm

NEW! ISO-GLU – Implantable glucose sensor

GLUcOSE SENSOR SPEcIFIcaTIONS

IN VITRO PRECISION Coefficient of variation (CV) ≤5%GLUCOSE RANGE 36 - 450 mg/dl (or 2-25 mM/L)RESPONSE TIME (sec) 100 - 300sIN VIVO CALIbRATION In vivo calibrationINTERFERENCE SPECIES Acetaminophen, ascorbic acid, uric acidLENGTH 5 cmSENSOR SIzE 0 .6 × 0 .7 mmREFERENCE ELECTRODe Ag/AgClPOLARIzATION VOLTAGE (V) 0 .65 -0 .7V vs . Ag/AgClSENSOR LIFE 3-4 months in solutions at room temperature under continuous polarization; 15-30 days in vivoSHELF LIFE 6 monthsOPERATION CONDITIONS 20° to 40° C (68° to 104° F)STORAGE CONDITIONS 10° to 25° C (50° to 77° F)

Measuring glucose in vivo over the long term is challenging and difficult . Previous measurement systems were limited to acute studies or a few days at best . WPI introduces a new kind of implantable glucose sensor based on a patented technology . This sensor provides a tool for researchers to directly detect glucose in chronic studies in vitro or in vivo . The sensor is fully compatible with WPI’s Apollo system .



ISO-NOPF100 100 µm Flexible NO Sensor, pkg of 2ISO-NOPF200 200 µm Flexible NO Sensor, pkg of 215810 Microsensor Adapter Cable

ISO-NOP007 7µmNitricOxideSensor(pkgof3) ISO-NOP3020 30µmSensorTips(2mmlength),pkgof3(requires#15810)ISO-NOP3005 30µmSensorTips(0.5mmfiber),pkgof3(requires#15810)15810 Microsensor Adapter Cable

ISO-GLU Implantable Glucose Sensor (pkg of 2) 15810 Microsensor Adapter Cable

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Hydrogen Peroxide SensorsHydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling . At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens . In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof . Victor Darley-Usmar, Univ . of Alabama, personal communication) . Despite the recognized importance of this oxidant in biology real-time measurements at low concentration have been difficult . The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range .

Two hydrogen peroxide sensors are currently available . The ISO-HPO-2 is a 2 .0 mm stainless steel sensor, with replaceable

membrane sleeves (#600012) and an internal refillable electrolyte (#100042) . The sensor is designed

for use in cell cultures and similar

applications . The graph below Illustrates the linearity of response of the sensor following successive increases in hydrogen peroxide concentration in solution .

The ISO-HPO-100 is a 100 micron tip diameter hydrogen peroxide

micro sensor designed for use in tissues and similar applications . The sensor design is based on a flexible “activated” carbon fiber sensing electrode coated with a proprietary membrane that enhances hydrogen peroxide detection .

both sensors incorporate WPI’s proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate reference electrode are encased within a single Faraday-shielded probe design . This design has been shown to enhance performance during measurements and minimizes overall sensor size .

ISO-HPO-2

Hydrogen Peroxide & Oxygen Sensor Guide ISO-HPO-2 ISO-HPO-100 ISO-HPO-100-L ISO-OXY-2 ISO-OXY-100

APPLICATION Cell Cultures, etc . Tissue/Microvessels Tissue bath Cell Cultures, etc . Tissue/Microvessels

SENSOR DIAMETER 2 .0 mm 100 micron 100 micron 2 .0 mm 100 micron

RESPONSE TIME <5 SEC (90%) <5 SEC (90%) <5 SEC (90%) <10 SEC (90%) <5 SEC (90%)

DETECTION LIMIT <100 nM to 100 mM <10 nM to 1 mM <10 nM to 1 mM 0 .1 % to 100% 0 .1 % to 100%

DRIFT <0 .1 pA/min <1 .0 pA/min <1 .0 pA/min <1%/min <1%/min

SENSITIVITY 0 .2 pA/nM 1 pA/nM 1 pA/nM N/A N/A

PHYSIOLOGICAL INTERFERENCE none Contact WPI Contact WPI none Contact WPI

ISO-NOP30-LISO-NOP70-LISO-HPO-100-LISO-OXY-100-LISO-NOPF200-L10L-shaped sensors for tissue bath studies

The ISO-NOP30-L is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI’s MYObATH) . The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study . The ISO-NOP70-L is similar in construction to the ISO-NOP30 but with the advantage of having a flexible tip (70 µm diameter) .

Micrometer

connect to Microsensor cable #15810


10 mm

3 mmcarbon fiber

FORT-100 Transducer

Bath≥ 10 mL

L-shaped NO sensor used with Myobath.

Oxygen SensorsTwo oxygen sensors are currently available . The ISO-OXY-2 is a 2 .0 mm stainless steel sensor, with replaceable membrane sleeves (#5378) and an internal refillable electrolyte (#7326) . The sensor is similar in design to WPI’s popular OXELP oxygen sensor (see page 58) .

The ISO-OXY-100 is a 100 micron tip diameter oxygen sensor . The sensor design is based on a flexible “activated” carbon fiber sensing electrode coated with a proprietary membrane that enhances oxygen detection .

Temperature Sensor

The temperature sensor (#ISO-TEMP-2) is based on a 2 .0 mm tip diameter high quality miniature platinum RTD (Resistance Temperature Detector) electrode . This design has been shown to provide greater accuracy, stability and interchangeability during temperature measurements than traditional thermistor and thermocouple sensors .

ISO-HPO-100



600011 ISO-HPOStartupKit(recommended with first purchase)ISO-HPO-2 2mm Shielded HPO Sensor & CableISO-HPO-100 100 µm HPO Sensor (requires #15810), pkg of 3600012 Replacement Sleeve Kit for ISO-HPO-2, pkg of 4100042 ISO-HPO-2 Electrolyte (10 mL)

ISO-OXY-2 2 mm Shielded Oxygen Sensor & CableISO-OXY-100 100 µm Oxygen Sensor, pkg of 35377 ISO-OXYStartupKit(recommended with first purchase)5378 Replacement Electrode Sleeve Kit, pkg of 47326 ISO2 Filling Solution (electrolyte)

ISO-TEMP-2 2mmPlatinumRTDTemperatureSensor(requires#15810) 15810 Microsensor Adapter Cable

ISO-NOP30-L NO Sensor, L-Shaped 30-micron (pkg of 3) ISO-NOP-70-L NO Sensor, L-Shaped 70-micron (pkg of 2) ISO-NOPF200-L10 NO Sensor, 200 µm Flexible L-shaped (pkg of 2)ISO-HPO-100-L HPO Sensor, L-Shaped 100-micron (pkg of 2) ISO-OXY-100-L Oxygen Sensor, L-Shaped 100-micron (pkg of 2) 15810 Microsensor Adapter Cable



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The ATP sensor is the first of a series of sensor-probes, designed for in vivo and in vitro experimental studies into the physiological and pathophysiological roles of the purines.

The device has been designed by neurophysiologists for fellow experimentalists and incorporates a number of practical features that have user-appeal . The novel design concept is based on the deposition of an active enzyme matrix, including glycerol kinase and glycerol-3-phosphate oxidase, onto the surface of a Platinum needle microelectrode . This is housed in a glass sheath and incorporates a simple connector device, enabling linkage to a potentiostat (WPI’s MicroC and Apollo series detectors) . The sensor is easy to use and exquisitely sensitive, with a broad linear range and fast response time . It is also easy to calibrate and capable of repeat use .

Benefits• Ready to use• Easy to use• Simple to calibrate• Exquisitely sensitive• Wide linear range• Reusable• Minimally invasive

WSa2001 SPEcIFIcaTIONSCONSTRUCTION Needle-shaped microelectrode enzyme-coated platinum/iridium wire

SENSOR LENGTH (tip) 2 mm X 50 µm (diam)

RESPONSE TIME 10-90% rise time ≤ 10 sec

SENSITIVITY 0 .5 nA per µM

LINEAR RANGE 0 .5 µM to 50 µM

SELECTIVITY specific for ATP versus UTP, ADP and Adenosine

SHELF-LIFE Dry: ≥6 months @ 4ºC Wet: ≤5 days

CALIbRATION Single point

RE-USE CHARACTERISTICS Subject to calibration

APPLICATIONS in vitro in vivo

SUITAbLE TISSUES brain, blood vessels gut, retina

Real-time measurement of ATP

Real-time Measurement of ATPplatinum wire50 µm diameter

enzyme layer

ATP + glycerol

ADP + glycerol-3-phosphate

glycerone phosphate + H2O2

glycerol kinase

glycerol-3-phosphateoxidase

[ATP] / µM

µM ATP

I / n

A40

30

20

10

00 25 50 75 100

1 5 10 50

100 s2 nA



WSa1001 ATP Sensor (package of 5) WSa1002 ATP Null Sensors (package of 5) Use of null sensor requires a second MicroC.

WSa2001 ATP Starter Kit (5 ATP sensors, 5 null sensors, reference electrode, calibration bottles) Use of null sensor requires a second MicroC.

WSa1003 Adenosine Sensor (package of 5) WSa1004 Inosine Sensor (package of 5) WSa2003 Adenosine Starter Kit (5 adenosine sensors, 5 null sensors, reference electrode, calibration bottles) Use of null sensor requires second MicroC. 300305 MicroC Adapter for ATP/Adenosine/Inosine Sensors

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Analog and digital circuits are separate, allowing you to combine ion concentrations with other types of signals (BP, force, etc.) with the data acquisition system of your choice.

You control signal conditioning — adjust filters, gains and smoothing to suit your signal, or use templates in the Data-Trax software.

Poise voltage is controllable by external command, allowing ramps, pulses, and techniques other than chronoamperometry, including cyclic voltametry, differential pulse polarography — or simply sweeping the poise voltage with a ramp to determine concentration of endogenous species.

Separate inputs for amperometric (free radical sensors), potentiometric (pH and other voltage sensors) and temperature.

analog vs. DigitalA not-so-obvious difference between this new device and its predecessor is that the Apollo 4000 is an inherently digital device and the Apollo 1000 is an inherently analog device . both have a current-to-voltage converter as their first electronic stage . both use filters after the I/V conversion, and both digitize to get the final signal onto the computer screen .

In the Apollo 1000 the current signal from the electrode enters the device and is converted to a voltage at a user-selectable gain . Thereafter it passes to a user-selectable filter block . After filtration the signal is buffered and sent to the output . WPI recommends that the analog signal then be presented to a Lab-Trax™ digital recorder where, under user control, software can be used to smooth, additionally filter and calibrate the signal . Lab-Trax is provided with Data-Trax™ software that runs on the user’s computer .

In the Apollo 4000 (and the two-channel Apollo 2000) the current signal from the electrode enters the device and is converted to a voltage at a user-selectable gain . The gain control in the Apollo 4000 is in the Apollo software . (In the Apollo 1000, the control is a physical switch on the device itself .) The signal then passes to a fixed 0 .05 Hz first order passive filter and is then digitized . Some manipulations to the digital signal then occur to compensate for the increase in response time caused by the heavy fixed filter . The digital signal is then split; one path is converted back to analog and sent to bNC connectors on the back of the unit . The other path takes the digital signal to an onboard Windows-based computer where the Apollo software further processes the signal by applying a non-adjustable 512-point Savitsky Golay smooth and automatic calibration .

There are several problems with the Apollo 4000 approach that do not exist in the Apollo 1000 . Raw data from the Apollo 4000 is the signal coming from the rear panel . This signal has already been processed to some fairly

severe extent and cannot be considered RAW . Further, the output from these connectors is very much noisier than the signal seen after processing by the Apollo software . If you are recording in a beaker and are not interested in anything beyond the change in concentration of the free radical, this scheme works well . If, however, you are examining the effect on other physiological parameters such as blood pressure or blood flow or developed force in a vascular ring, you are required to take the analog out from the Apollo 4000 and feed it to an external data system or chart recorder where the facility to process the noise may not exist or at least not be obvious .

In the Apollo 1000 the user has complete control of the bandwidth and the strength of the smoothing . In the Apollo 4000 these parameters are fixed .

If you are looking at Nitric Oxide from low current output sensors it becomes necessary to sacrifice bandwidth and rise time to get the noise down . This is a time-honored and inescapable tradeoff . In free radical measurement, noise levels are directly related to detection limit — lower is better . but what if you are looking at a species like oxygen or some other species where output currents are larger? In the non-user-controllable environment of the Apollo 4000 you have permanently sacrificed bandwidth for noise . Your signal is really quiet but any hope of doing fast kinetics or stop flow experiments is gone . In the Apollo 1000 scheme the bandwidth of higher current signals can be increased at the expense of additional noise where it does not matter .

Poise Voltage controlThe Apollo 4000 is able to create poise voltage from 0 to +2V . For species requiring negative poise voltage, the wiring in connectors needed to be changed to reverse the polarity . beyond this, poise voltage was set from within the Apollo software and was limited to DC .

The Apollo 1000 has a poise voltage range of 0V~ ±2V . In addition the Apollo 1000 can allow the poise voltage to be driven by an external signal . The split supply (+ and - voltages) together with the external command allow cyclic voltametry, differential pulse polarography and a host of other electro-chemical techniques not possible with DC poise voltages . In this connection, if the poise voltage is changing with time the preferred medium of display is the XY plot . (The Apollo 4000 software is limited to linear (Y,T) displays .) Data-Trax v2 .0 offers XY as well as other features that make it better suited to analysis and display of electrochemical data .

Potentiometric inputIn addition to its free radical sensor input, the Apollo1000 has a separate potentiometric input . This is a 1015 input, capable of accepting pH electrodes directly without buffer amps . because of the linear calibration feature in Data-Trax, other units such as pCa or pK can be easily set up even if the slope of the sensor is unknown .

Apollo 1000one-channel Free Radical Analyzer



aPOLLO1000 1-Channel Free Radical Analyzer System Includes meter, one sensor (specify), temperature sensor and start-up kit

INST-aPOLLO Installation and TrainingLaB-TRaX-4 4-Channel Data Acquisition System

See sensors on pages 80-85.



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The APOLLO 4000 is an optically isolated 4-channel free radical analyzer designed for the detection of a variety of redox-reactive species of biomedical importance . The electrochemical (amperometric) detection principle used enables detection of a broad range of redox-reactive species with unsurpassed accuracy and sensitivity .

APOLLO 4000 incorporates a powerful single board computer software that enables real-time display and data-acquisition of experimental data . An extensive graphical user interface (GUI) based on a full color LCD monitor with touch-screen-control allows complete control and programming of all detection and data-acquisition parameters to be made at a single touch . Alternatively, the instrument can be controlled using a standard keyboard and mouse (included) .

APOLLO 4000 is designed for use with WPI’s range of nitric oxide, hydrogen peroxide and oxygen sensors . The user simply plugs the required sensor into any one of the input channels located on the instruments main front panel and then selects the detection and acquisition parameters using the software . Each channel is also provided with an independent temperature input port that allows real-time monitoring of temperature using the available temperature sensors .

l 4-channel isolated architecture allows simultaneous measurement of up to 4 different species in the same preparation or simultaneous measurement in 4 different preparations.

l Ultra low noise and fast response times allow you to record from a variety of sensors

l Current measurement range from 100 fA to 10 µA (4 ranges) permits wide dynamic range for detection.

l Real-time detection using electrochemical microsensors.

l Measure NO from < 0.3 nM to 100 µM.

l Measure hydrogen peroxide < 10 nM to 100 mM.

l Measure oxygen from 0.1% to 100%.

l Measure Independent temperature and display on all channels.

l Slim-line desktop control unit can be used on the bench or rackmounted

Apollo 4000

World’s first fully-integrated No / Free Radical Analyzer

aPOLLO 4000 SPEcIFIcaTIONSNUMbER OF CHANNELS 8 (4 Sensor + 4 Temperature)A/D RESOLUTION 24 bitSAMPLE SPEED 1 s/s to 50 s/sPOISE DAC VOLTAGE RANGE 0 – 2VPOISE VOLTAGE SETTING ACCURACY 50 µV

CONCENTRATION MEASUREMENT Ranges of Measurement 10nA, 0 .1µA, 1µA, 10µA Signal to Noise Ratio 106 db (100,000 : 1) Limit of Detection 50 fA Absolute Accuracy 1%

TEMPERATURE MEASUREMENT Measurement Element Platinum RTD Accuracy 0 .1 % Degree Celsius Interchangeability Error 0 .2 % Degree Celsius

USER INTERFACE Display 12" Diagonal Color LCD Touch screen Resistive Resolution SVGA (600x800); 16,777,215 colors Operating System Windows XP Professional Compatibility (Interface) Printer port, 2 USb ports, 10/100 base T Ethernet, PS2 keyboard and mouse

DIMENSIONS (H x W x D) 13 .3 x 42 .5 x 25 .4 cm (5 .25-in . x 16 .73-in . x 10-in .) (compatible with 19-in . rack)

WEIGHT 5 .9 kg (13 lb)

NOTe: Due to continued development, the functionality and the performance parameters of the system may be changed without a notice.

Now Includes 2 Sensors Free!



aPOLLO 4000 SystemSystem includes the APOLLO 4000 instrument and associated software, color LCD display, temperature sensor, cables, keyboard, mouse, manuals.aPOLLO4000-4 4-Channel Free Radical Analyzer System Includes one sensor (specify) and one start-up kitaPOLLO4000-2 2-Channel Free Radical Analyzer System Includes one start-up kit with purchase of sensor (specify)UPGRaDE-aPOLLO ConvertsAPOLLO4000-2toAPOLLO4000-4

See sensors on pages 80-85

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l Four port (WPI #NOCHM-4) chamber accommodates WPI’s 2-mm sensors for nitric oxide (ISO-NOP), oxygen (OXELP), hydrogen peroxide, and WPI’s KWIK-TIP ion selective electrodes in combination with WPI’s 2 mm Dri-Ref™ reference electrodes .

l Two additional top ports for injection of reagents using WPI’s MicroFil™ syringe needles

l Closed chamber design greatly reduces the surface area of the solution exposed to air

l One top port and up to three side ports configuration provides adequate space for convenient sample and electrode manipulation

l Temperature control through an external circulating bath

l The chamber can be used for nitric oxide and other species calibration at temperatures from 4-40 ºC

The measurement of NO and other reactive gases dissolved in solutions will be underestimated in stirred conditions if the solution is allowed to equilibrate with air . In the case of NO, accelerated decomposition occurs as the result of diffusion of NO from the solution into the gas phase and the reaction of NO with oxygen . This reaction with oxygen makes a significant and variable contribution to NO decomposition, and hence accuracy of measurement, at concentrations of NO between 0 .1-5 µM . These problems can now be eliminated with the use of WPI’s two-port NOCHM or four-port NOCHM-4 closed chambers . The chambers consist of a close fitting cap through which a NO probe (ISO-NOP) or other electrode can be inserted . When the probe is in place and the cap is fitted to the chamber the surface area of the solution exposed to air is greatly reduced . Up to three optional side ports are also provided through which an oxygen electrode* (e.g., OXELP), WPI’s hydrogen peroxide, or KWIK-TIP ion selective electrodes in combination with WPI’s 2 mm Dri-Ref™ reference electrodes can be inserted .

The multi-port measurement chambers can be conveniently temperature-controlled by circulating water through the outer sleeve of the chamber using an appropriate heating/cooling circulator bath . The inner volume of the chamber (and hence sample volume) can be continuously adjusted in volume from 1 .0 mL to 3 .0 mL and is suitable for most cell suspension experiments .

REFERENcEP. S. Brookes, E. P. Salinas, K. Darley-Usmar, J. Eiserich, B. A. Freeman, V. M. Darley-Usmar, P. G. Anderson. “Concentration dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release” . J. Biol. Chem. 275, 20474-9 (2000) .

Multi-Port Measurement chamberTemperature stabilized four-port closed chamber for measurements of nitric oxide, oxygen hydrogen peroxide and other species in cell culture

MULTI-PORT MEaSUREMENT cHaMBERS

VOLUME OF SAMPLE 1-3 mL

SAMPLE INJECTION PORTS 2 (top)

NUMbER OF ELECTRODE PORTS 4

ELECTRODE COMPATIBILITY Nitric Oxide Electrode ISO-NOP Oxygen Electrode* OXELP* Hydrogen Peroxide Electrode ISO-HPO-2 Calcium Electrode KWIKCAL-2 Hydrogen Electrode KWIKH-2 Potassium Electrode KWIKPOT -2 TPP (tetraphenylphosphonium) Electrode KWIKTPP-2 Dri-Ref Electrode DRIREF-2 SUPER-Dri-Ref Electrode SDR2

TEMPERATURE RANGE OF CIRCULATING WATER 4-40 ºC

Water inlet and outlet require 1/4-in. ID tubing* Caution: Not recommended for low oxygen concentration (<1%) measurements.



NOcHM-4 Four-Port Closed Chamber for use with WPI’s 2 .0 mm electrodes (e.g., ISO-NOP and OXELP, etc .) NOcHM-P Spare Plug-adapter for ISO-NOP nitric oxide electrode 800100 Spare Center Chamber Gasket (package of 2)



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GS-Nitroso-N-acetyl-D,L-penicillamine

SNAP is a stable green crystalline S-nitrosothiol compound that mimics the action of nitric oxide in vivo . It has vasodilatory properties and has been shown to relax isolated bovine coronary artery

rings by activating soluble granulate cyclase . This reagent also actuates apoptosis in mouse thymocytes and has been accounted for reversible inactivation of protein Kinase C . SNAP can be used for calibration of all WPI NO sensors . Call for details .

M .W . 220 .2

Purity > 98% by NMR or TLC

REFERENcESS. C. Askew, et al. Bioorg. Med. Chem. 3, 1 (1995) .K. Fehsel, et al. J. Immunol. 155, 2858 (1995) .R. Gopalakrishna, et al. J. Biol. Chem. 268, 27180 (1993) .E. Southam, J. Garthwaite, Neurosci. Lett. 130, 107 (1991) .P. J. Henry, et al. J. Pharmacol. Exp. Therap. 248, 762 (1989) .

GSNOS-nitrosoglutathione

GSNO has been identified in vivo as a potential storage and transport vehicle for NO in the body . GSNO has been used in clinical trials to treat a form of preeclampsia and to prevent platelet aggregation . It also has considerable potential as NO donor in medicine .

M .W .336 .3 • C10H16N4O7S • Purity > 98% • Soluble in water or DMSO • Storage: -20°C

REFERENcES A. R. Butler, Anal. Biochem. 249:1-9 (1997) .D. Nikitovic, J. Biol. Chem. 271(32), 19180-19185 (1996) .D. R. Noble, Nitric Oxide 4:392-398 (2000) .D. L. H. Williams, Acc. Chem. Res. 32:869-876 (1999) .

O

N

S

H3C CH3

COOH

NHAc

NH

O

HO2C CO2H

NH2

SNO

O

HN

Pre-polarizerKeep extra NO sensors ready to use

ISO-NOP Rejuvenator



After an ISO-NOP 2-mm sensor is used for long periods, sensitivity may become reduced and response time may increase . This little device can restore ISO-NOP performance to original levels by applying an electric waveform for a few seconds . 9v alkaline battery included .

JUV ISO-NOP Rejuvenator

SNaP25 SNAP, 25 mg vialSNaP50 SNAP, 50 mg vialSNaP100 SNAP, 100 mg vial

GSNO-50 GSNO 50 mg vialGSNO-100 GSNO 100 mg vialGSNO-500 GSNO 500 mg vial

Achieve a stable background current quickly . This small battery-powered device applies a potential to the NO electrode equivalent to the potential applied by the ISO-NO meter . Consequently, a sensor which has been connect-ed to the activator may be transferred to the meter for immediate use . For use with all WPI NO electrodes.

NSa-3 ISO-NO Activator (for three sensors)

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assay Principle: Luminol reacts with superoxide and other reactive oxygen species resulting in a luminophore that has an emission peak at ~425 nm . The intensity of the chemiluminescence is proportional to the amount of superoxide or reactive oxygen species in the sample . The superoxide reaction shown above exemplifies chemiluminescence production from luminol .

The kit is based on a proven assay which is used to quantify gross cellular production of reactive oxygen species including; superoxide, hydroxyl radical, peroxynitrite and hypochlorite . When the other reactive oxygen species are mostly derived from superoxide, this assay provides an indirect measure of cellular superoxide production . Indeed, luminol assay is one of the most important methods in the analysis of metabolic activities of phagocytes and is widely used in biomedical research .

WPI Enhancer: A number of compounds are known to increase the intensity of luminescent emission from luminol . The WPI SuperLuminol kit utilizes a patented non-toxic enhancer to provide ~50 times increase in luminescence emission intensity from cells . The figure above shows oxidative bursts from phagocytes in whole blood (after 400 times dilution) induced by phorbol myristate acetate (PMA) in the absence and presence of the enhancer . Significantly lower amounts of reactive oxygen species can be assayed when using the enhancer .

Kit contents: The WPI SuperLuminol kit contains a complete reagent set to assay superoxide/reactive oxygen species for cell cultures, isolated phagocytes, un-isolated phagocytes in whole blood, and other biological samples . The kit can be purchased with or without a chemical stimulant/agonist (PMA) for superoxide/reactive oxygen species production in cells . The kit includes the following reagents:

• Luminophoric Reagent: Luminol• Assay buffer: HbSS• Enhancer• KO2 (qualitative test control reagent)• PMA (optional)• PMA solvent (dimethyl-sulfoxide, optional)

Benefits:• Simple and extensively tested method with an increased sensitivity than chemical methods• Further improvement of sensitivity using a proprietary enhancer• Minimal cellular toxicity from luminol and the enhancer

SuperLuminolSuperoxide/Reactive Oxygen Species Determination Kit based on Enhancer-Amplified Luminol Chemiluminescence

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PeroxiLuminol Hydrogen Peroxide Determination Kit Based on Peroxidase-Catalyzed Luminol Chemiluminescence

Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling . At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens . In the development of human disease uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes such as xanthine oxidase can occur (Prof . Victor Darley-Usmar, University of Alabama, personal communication) . Hydrogen peroxide concentrations at µM level elicit a broad spectrum of cellular responses ranging from mitogenic growth stimulation to apoptosis and necrosis . PeroxiLuminol is a hydrogen peroxide chemiluminescence bioassay kit developed by WPI to provide an accurate analytical tool by which to measure hydrogen peroxide formation in the living system . The kit is designed to compliment WPI’s new range of hydrogen peroxide electrochemical sensors . When combined together these detection methods will greatly aid hydrogen peroxide research .

assay Principle: Horseradish peroxidase (HRP) reacts with hydrogen peroxide to form reactive Compound I and Compound II, which in turn oxidize luminol with concomitant production of chemiluminescence (see reaction scheme below) . The PeroxiLuminol Kit has been optimized so that hydrogen peroxide concentration is linearly proportional to luminescence intensity . Our formulation assures that luminescence intensity doesn’t decay too quickly so that measurement can be made accurately and precisely . The slow decay rate also allows luminescence measurement with simple luminometers that may not include injection/mixing devices . The figure above shows hydrogen peroxide standards as assayed by the HRP-catalyzed luminescence method .

Benefits

• Determine hydrogen peroxide concentration in minutes .• Simple and extensively tested method with an increased sensitivity .• More stable signals .• Inexpensive measurement instrument .

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SUPERLUMINOL Superoxide/ReactiveOxygenSpeciesDeterminationKitSUPERLUMINOL-II SuperLuminol+PMA(1mg)/solvent

The kits provide material for 100 assays in cuvette or test tubes (assay volume: 1 mL/assay).

Note: This kit is designed to assay hydrogen peroxide in cell cultures. Samples with high hemoglobin content and/or metal content may not be suitable for assay. Due to continued development, the kit format and performance parameters may be changed without a notice. Contact WPI for further information.

PEROXYLUMINOL HydrogenPeroxideDeterminationKit

This kit provides material for 100 assays in cuvettes or test tubes (assay volume: 1 mL/assay).



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Glutathione (GSH), a tripeptide antioxidant, plays multiple biological functions . It is involved in detoxification of harmful molecules, such as reactive oxygen species (i.e., hydrogen peroxide and hydroperoxides) through glutathione peroxidaes, and xenobiotics through glutathione transferase . It is also involved in signal transduction, gene expression, apoptosis and interaction with nitric oxide . Glutathione antioxidant status is frequently measured in physiology and pathophysiology .

Assay principal: GSH reacts with Ellman’s reagent (5,5’-dithiobis(nitrobenzoic acid) or DTNb) to produce a chromophore TNb with maximal absorbance at 412 nm and oxidized glutathione GSSG . Tietze formulated an enzymatic method

to regenerate GSH using Glutathione Reducatse and NADPH, to provide specificity and improved sensitivity for the assay . The reaction mechanism is depicted schematically in the following figure . Since glutathione reductase is used, the amount of glutathione measured represents the sum of reduced and oxidized glutathione in the sample ([GSH]t = [GSH] + 2 x [GSSG]) . The rate of absorbance change (∆A412nm/min) is made to be linear for the convenience and consistence of measurement, and is linearly proportional to the total concentration of GSH . The concentration of an unknown (sample) is determined by calculating from the linear equation generated from several standards of glutathione .

GSH aSSaY KIT Quantitative Determination of Glutathione Using Tietze’s Enzymatic Recycling Method

Other Benefitsl Formulated for convenient and fast assay for biological samples —

3 minutes/assay at room temperature .

l Use simple spectrophotometers or microtiter plate readers .

l No interference from cysteine and metal ions .

l Wide assay range from 0 .1 µM to 30 µM in sample (dilution is required for samples with higher GSH concentration .)

l Kit includes all required reagents and standards .

Selective References1 . Sies H ., “Glutathione and its role in cellular function”, Free Rad.

Biol. Med. (1999), 27, 916-921 .

2 . Griffith, O . W ., “biological and pharmacologic regulation of mammalian glutathione synthesis”, Free Rad. Biol. Med. (1999), 27, 922-935 .

3 . Tietze F ., “Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues”, Anal. Biochem. (1969), 27, 502-522 .

NADPH GR

TNB GSTNB TNB(λ412nm) (λ412nm)

GSHt ↔ ∆TNB/min ↔ ∆A412nm/min

GRNADPH

GSHGSSG DTNBGSHt

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GSH-KIT Total Glutathione Determination Kit

50 cuvette assays/200 microplate well assays

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GNITRaLYZER™-II Nitrate to Nitrite Reduction KitThis kit uses disposable copper-coated cadmium granules to convert nitrate into nitrite in minutes, with consistent ~100% efficiency . It is compatible with both WPI’s GRIESS REACTION NITRITE KIT and ISO-NOP electrochemical nitrite assay method .

Nitrate and nitrite are end products of short-lived nitric oxide in-vivo . Nitrite concentration can be measured by a number of well-established methods . Nitrate assay requires conversion into nitrite before the measurement . Copper-coated cadmium is the most popular chemical reducer among researchers . Alternative methods based on enzymatic reduction of nitrate require much longer reaction times and their conversion efficiency decreases at high nitrate concentrations . Advantages of cadmium based method include stability upon storage and invariability between different lots .

Benefitsl ~100% conversion efficiency in minutes (up to 100µM nitrate) .

l Disposable – used only once to yield consistent results .

l Conversion takes place at room temperature .

l Compatible with both GRIESS REACTION NITRITE KIT and ISO-NOP electrochemical nitrite assay technique .

l Scalable – smaller volumes for hard-to-obtain biological samples (12 µL minimum sample volume) .

l Kit includes all required reagents, including deproteinization reagents .

l Extensively tested in WPI’s laboratory .

GRIESS REacTION NITRITE KITQuantitative Determination of NitriteThis kit uses the standard colorimetric Griess Reaction to measure nitrite concentration in biological samples . When nitrate or total nitrate plus nitrite (Nox) is measured, samples must first be treated by NITRALYzER-II Kit to convert all nitrate into nitrite .

Spectrophotometric quantification of nitrite using Griess reagents is simple, reliable and cost-effective . It is widely used in numerous laboratories around the world and remains the method of choice for some applications . Nox assay requires reduction to nitrite before Griess Reaction . Nitrate concentration can then be determined by calculating the difference between Nox concentration and nitrite concentration .

Benefitsl Simple (fast) and reliable, suitable for large number of samples .

l Low cost .

l 0 .3 µM – 100 µM assay range (assuming that no deproteinization is required) .

l Kit includes all required reagents, including deproteinization reagents .

l Assay at room temperature .

l Extensively tested in our laboratory .

Spectrophotometer and semi-micro cuvettes are required for the assay (see Spectroscopy Section). Alternatively, a microtiter plate reader can be used for absorbance measurement.

Copperized Cadium Nitrate Reducer

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NITRaLYZER II Nitrate to Nitrite Reduction Kit

Kit contains material for 100–500 conversions

GRNK Griess Reaction Nitrite Kit, 100-500 Nitrite tests

GRNK-II GRNK + NITRALYzER-II, 100-500 Nox tests



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Measures oxidizable compounds such as catecholamines (epineph-rine, norepinephrine, dopamine), indolamines (serotonin, melatonin), ascorbic acid and Fe (II) with exquisite sensitivity, low noise and site specificity

™

MicroC, WPI’s low cost and elegant instrument for electrochemical detection using carbon microelectrodes, will record the presence and concentration of oxidizable biological compounds in vivo and in vitro. It also features inherently low noise and a sensitivity of 1 millivolt per picoampere of oxidation current . Response time to quantal cate-cholamine release is less than 1 millisecond . When used with carbon fiber microelectrodes, redox current can be recorded over a range of

Advanced Neurotransmitter

Detection

1-picoampere to 2 microamperes . The built-in carbon electrode activation feature allows the easy renewal of electrode sensitivity . In addi-tion, MicroC features a low-pass filter and the option of applying DC potential externally . A wide range of compounds can be detected: dopamine, epinephrine, norepinephrine, sero-tonin, ascorbic acid, etc . Other compounds, such as glutamate, glucose, acetylcholine and alcohol, can also be detected with MicroC using enzyme-modified biosensors .

See Application Notes, “Carbon Fiber Microelectrodes”, available as a PDF file which may be downloaded from WPI”s web site .

The MicroC Potentiostat is supplied with a carbon electrode probe, with 5 feet triax cable, which accepts 0 .79 mm connector pin, and a reference electrode with a 4 mm Ag/AgCl half cell (see page 27) . For applications where smaller half cells are needed, please call WPI for more information .

ReferencesG. A. Gerhardt, “Nafion-coated electrodes with high selectivity for CNS electrochem-istry” Brain Research, 290: 390-395 (1984) .

R. M. Wightman, et al., “Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells .” Pro. Nat’l Acad. of Sci. 88: 10754-58, (1991) .

Z. Zhou and S. Misler, “Action Potential-induced Quantal Secretion of Catechola-mines from Rat Adrenal Chromaffin Cells”, J. Biol. Chem. 270; 3498-3505, (1995) .

MIcROc SPEcIFIcaTIONSMETHOD 2 electrode, DC potentiostatAPPLIED POTENTIAL 0 .65 V, variable ± 2 .5 V CURRENT RANGES 2000 pA, 20 nA, 200 nA, 2 µAbANDWIDTH 1 .67 Hz, 167-1000 HzNOISE < 1 pADISPLAY 31⁄2-digit LCD display, ±2 VRECORDER OUTPUT ± 4 .5 voltsRISE TIME < 1 millisecondELECTRODE PRObE/CAbLE LENGTH Triax shielded, 5 feetPOWER Six 1 .5 volt alkaline batteries (included)bATTERY LIFE > 1000 hours, est .SHIPPING WEIGHT 4 lb (1 .8 kg)

Records redox currents with carbon fiber microelectrodes

Use with WPI electrodes — see next page



MIcROc PotentiostatMIcROcP Replacement Probe for MicroC

OPTIONaL accESSORIES300305 ATP Adapter (0 .031" pin to 1 mm socket)

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● Sensitive, renewable/durable and economical carbon fiber electrodes for electrochemical detection of oxidizable compounds

Carbon fiber microelectrodes have been used in both the detection of oxidizable compounds (Gonon, et al., 1978; Cahill and Wightman, 1995) and extracellular single-unit recording (Armstrong-James and Millar, 1979). WPI’s ultra-sensitive and low-noise carbon fiber (CF) electrodes can be applied, with our Micro-C Potentiostat or similar instru-ments, in the electrochemical detection of catecholamines (epineph-rine, norepinephrine and dopamine), indolamines (serotonin, 5-HT or melatonin), ascorbic acid, Fe (II), and other oxidizable compounds.

CF electrodes (diameter of 10 or 30 µm) respond with an excel-lent linearity to the oxidizable compounds (Fig. 1) and can detect the compounds as low as 0.2 nM. While the shorter (25-100 µm) CF electrodes are suitable for in vivo amperometric and voltammetric measurements, the longer CF electrodes provide higher sensitivity and are especially useful for the in vitro studies (amperometric or differential pulse voltammetry). When used with the Micro-C Potentiostat, these CF electrodes can be activated and renewed in sensitivity for multiple use. The selective detection of catecholamines can be achieved with our Nafion-coated CF electrodes. For selective detection of 5-HT and ascorbic acid, please contact WPI for more information.

ReferencesP. S. Cahill, R. M. Whightman, Anal. Chem., 67, 2599-2605 (1995).F. Gonon, et al., Hebd Seances Acad. Sci. Ser. 286, 1203 (1978).M. Armstrong-James, J. Millar, J. Neurosci. Methods, 1, 279 (1979).

Carbon Fiber Microelectrodes

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Fig. 1 — Excellent linearity in the response of carbon fiber electrode (CF30-500) to dopamine recorded on Micro-C. Courtesy: Drs. D. Yeomans and X.-T. Wang, University of Illinois at Chicago.

Fig. 2 — Extracellular recording using a carbon electrode in CA1 region of the hippocampus in an anesthetized rat shows ultra-low noise (-<5 µV-). Courtesy: Dr. Carolyn Harley of Memorial University, Newfoundland, Canada.

Gold-plated connector pin0.031 in. (0.79 mm)

1 mm O.D. glass pipette or 2 mm plastic tubeglass insulation

cement

30 or 10 carbon fiber

25-1000 m

Carbon fiber microelectrodes, approximately 25-50 mm (1-2 in. ) long, feature a gold-plated pin for connection to the probe.



CARBON FIBER MICROELECTRODES, UNCOATED Diameter Length (pack of 5)CF10-25 * 10 µm 25 µmCF10-50 10 µm 50 µmCF10-100 10 µm 100 µmCF10-250 10 µm 250 µmCF10-500 * 10 µm 500 µmCF30-25 * 30 µm 25 µmCF30-50 * 30 µm 50 µmCF30-100 30 µm 100 µmCF30-250 * 30 µm 250 µmCF30-500 * 30 µm 500 µmCF30-1000 * 30 µm 1000 µmCARBON FIBER MICROELECTRODES, NAFION-COATED* Diameter Length (pack of 5)CFN10-25 * 10 µm 25 µmCFN10-50 * 10 µm 50 µmCFN10-100 10 µm 100 µmCFN10-250 * 10 µm 250 µmCFN10-500 * 10 µm 500 µmCFN30-25 * 30 µm 25 µmCFN30-50 * 30 µm 50 µmCFN30-100 * 30 µm 100 µmCFN30-250 * 30 µm 250 µmCFN30-500 * 30 µm 500 µmCFN30-1000 * 30 µm 1000 µm* Built to order — allow up to 4 weeks manufacturing time.



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GOxyMini & OxyMicroA new generation of fiber optic oxygen sensors based on luminescence lifetimeThe OxyMini system is optimized for process control and biotechnology applications . The OxyMicro is designed for biological research applications including implanting into tissues, cell cultures, profiling of biofilms and sediment related bioassays . The measurement principle of the sensor system is based on the detection of oxygen concentration as a function of luminescence lifetime either in dissolved or gaseous phase environments .

OxyMini and OxyMicro Benefits l Oxygen is not consumed during the experimentl Immune to electrical and magnetic interferencel Excellent long-term stabilityl No lengthy polarization necessary (e.g., as Clark-type oxygen electrodes require)l Fast response time < 0 .5 s for MicroTip sensorsl Probe size of MicroTip sensors as small as 50 µm l Measurement is feasible in dry gasl Optical isolation of sensor tip available for fluorescent or photosynthetically

active samples

Measurement PrincipleConventional fiber-optic oxygen sensor systems based on intensity measurements are limited in their accuracy by light source stability and ambient light fluctuations . Using a luminescence lifetime detection, measurements are not affected by light source stability, intensity fluctuations caused by fiber bending or changes of the optical properties of the sample (turbidity, refractive index, coloration, etc .) . These advantages make WPI’s OxyMini and OxyMicro the most advanced and reliable fiber-optic oxygen system available .

calibration: The sensors can be calibrated by a simple two point calibration, 100% air-saturation and 0% air saturation .

OxyMini and OxyMicro oxygen meters: The OxiMini and OxyMicro fiber optic oxygen meters are compact, easy to transport . The instruments are designed for in/outdoor use and can be connected to a PC via a RS232 interface . Data can be visualized, analyzed and stored with the supplied software . A full range of sensors covering most biomedical applications are available .

OxyMicro systemsThe OxyMicro is a single channel fiber optic oxygen meter for WPI’s fiber optic oxygen microsensors . Applications include:

l Oxygen profiles of marine sediment, soils, or tissue l Implantation into living tissue (e.g., heart or muscle tissue)l Control of cell culture media in biotechnology .

MicroTip

The MicroTip (WPI #501656) is a needle-type (27 ga .) oxygen micro sensor designed for applications where a small tip size of 50 µm and a fast response time (t90) of 1 s are necessary . The oxygen sensitive sensor tip consists of 140 µm fiber tapered to a 50 µm tip . The sensor is housed inside a stainless steel needle of 22 mm length and 0 .4 mm diameter . This allows penetration through a septum rubber or similar material . These sensors are ideal for oxygen profiling in sediments and biofilms .

MicroFlow

The MicroFlow fiber optic oxygen sensor (WPI #501657) is a miniaturized fiber optic chemical sensor optimized for fast response time (t90 < 1 sec in gases, < 5 sec in liquids) . The tiny probe has a tip size of 50 µm and is integrated in a T-shape flow cell for easy connection via Luer-Lock adapters to external tubings . Liquids (like water, blood, etc .) can be pumped through the cell .

MicroImplant

The MicroImplant fiber optic oxygen sensor (WPI #501658) is a implantable probe (IMP) with a tiny probe tip size 50 µm, an exposed fiber length of 5-mm and a jacket diameter of 900 µm . The IMP sensor was successfully implanted in crabs, fishes and soil .

OxyMini systemsThe OxyMini is a single-channel fiber optic oxygen meter for WPI’s fiber optic oxygen minisensors . These sensors are based on 2 mm polymer optical fibers and have a length of 2 .5 m . A wide range of applications is possible with these sensors .

l Process control: bottling plant in breweries and quality control of packagesl biotechnology: Control of cell culture media and non-invasive control of

bioreactorsl Implantation of oxygen sensors into soil and trees .

MiniTip

This oxygen dipping probe (WPI #501641) has a tip diameter of 4 mm and consists of a polymer optical fiber, with a oxygen sensitive coating . The MinTip’s range is 0 to 100% . This robust sensor has a response time (t90) of approximately 40 s .

MiniFlow

The MiniFlow oxygen probe (WPI #501642) is a miniaturized fiber optic chemical sensor integrated in a T-shape flow through cell . The standard T-shape flow cell can be easily connected via Luer-Lock adapters to external tubings . Liquids (e.g., water, blood, etc .) can be pumped through the cell . The sensor has a response time (t90) of approximately 40-s and an excellent long-term stability .

MiniFoil

WPI offers the sensor material on a 1 cm2 support disk made of polyester . This material can be glued, for example, inside glass vials and the oxygen concentration can be measured non-invasively and non-destructively from outside through the wall of the flask . A plastic fiber optic cable (WPI #501644, WPI #501645) is used to illuminate the sensor foil . The wall of the flask must be transparent/non-fluorescent . Response time (t90) of approximately 50 s . The material can be implanted into animal tissues or custom-made housings .

Contact WPI or visit Web site for additional details.

MiniTip MiniFlow MiniFoil MicroTip MicroFlow MicroImplantMeasurement Rangedissolved/gaseous

0-45 ppm, 0-100%0-760 mmHg

0-45 ppm, 0-100%0-760 mmHg

0-45 ppm, 0-100%0-760 mmHg

0-45 ppm, 0-100%0-760 mmHg

0-45 ppm, 0-100%0-760 mmHg

0-45 ppm, 0-100%0-760 mmHg

Response Time [t90]dissolved/gaseous

40 s10 s

40 s10 s

40 s10 s

< 2 s< 0 .5 s

< 2 s< 0 .5 s

< 2 s< 0 .5 s

Sterilization (EtOH, H2O2)autoclavable (130°C, 1 .5 atm)

YN

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Drift (100,000 datapoints, 20°C) < 0 .1% < 0 .1% < 0 .1% < 0 .3% < 0 .3% < 0 .3%

Accuracy (20°C) 0 .2%

Resolution (20°C) 2 .75 ±0 .01 ppm, 9 .00 ±0 .05 ppm, 220 ±0 .15 ppm, 45 .0 ±0 .25 mmHg, 150 ±0 .75 mmHg, 375 ±2 .6 mmHg

Temperature Range -10°C to 50°C

Probe Assembly Length 2 .5 m



MINISENSOR SYSTEMOXY-MINI-AOT Fiber-opticOxygenMeterforMinisensors*

MINISENSORS (not interchangeable with Microsensors)501641 MiniTip,fiber-opticoxygensensor501642 MiniFlow,flow-throughcellwithintegratedplanaroxygensensor501643 MiniFoil,planaroxygen-sensitivefoils,1x1cm(1cm2)

REQUIRES#501644501644 Polymeropticalfiberwith1SMAconnector

MICROSENSOR SYSTEMOXY-MICRO-AOT Fiber-opticOxygenMeterforMicrosensors*

MICROSENSORS (not interchangeable with Minisensors)501656 MicroTip,needle-typehousingfiber-opticoxygensensor501657 MicroFlow,flow-throughhousedoxygenmicrosensor501658 MicroImplant,implantableoxygenmicrosensors

*Meter contains two analog outputs and one trigger input

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Dissolved oxygen meter & oxygen electrodeElectrically isolated, the electrode can function in recording situations where ground-referred electrodes would fail

Isolation prevents adverse interaction with other electrodes and instruments . Highly accurate and stable ISO2 measures oxygen concentrations in aqueous solutions and in gas mixtures . Measurement modes are percent oxygen, parts per million, and oxygen reduction current in nanoamperes .

The lower detection limit (DL) for ISO2 (in gaseous or in liquid phase) is 0 .1 ppm or 0 .1% . Oxygen concentra-tion around 0 .5 ppm or 0 .5% and up can be routinely measured in the gaseous or in the liquid phases .

DL for a particular sensor tends to be different in gaseous and liquid phases when the baseline noise level is also different . In the case of ISO2, however, the baseline noise level is very close whether the electrode is immersed in solution or used in the gaseous phase, so DL for the ISO2 remains relatively close in either phase .

The small tip size (2 mm diameter) and low oxygen consumption of the OXELP electrode make it ideal for measurements in vivo or in vitro . With a T-Adapter (#5399), the sensor probe can be used also for contin-uous-flow monitoring of oxygen in small fluid volumes . OXELP also features a fast response time, typically 10 seconds, and the rugged stainless steel sleeve covering the sensor is autoclavable . Optional bNC-to-double banana adaptor (#13347) and bNC cable (#500184) allow ISO2 to be connected directly to your chart recorder .

l Measures gas and dissolved oxygenl Low O2 consumptionl 2 mm tip

ISO2 SPEcIFIcaTIONSMEASUREMENT MODES % O2: 0-100% ppm: 0-20 Current: 0-200 nA

RESOLUTION 0 .1 ppm

ACCURACY ± 1 .5%

RECORDER OUTPUT 1000 Ω resistance for chart recorders

DISPLAY 3 .5-digit LCD

POWER 2 nine-volt alkaline batteries, supplied

bATTERY LIFE 1000 hours, estimated

DIMENSIONS 8 x 4 x 2 inches (20 x 10 x 5 cm)

SHIPPING WEIGHT 5 lb (2 .3 kg)

OXELP OXYGEN ELEcTRODETIP DIAMETER 2 mm

STAINLESS STEEL SLEEVE 66 mm

OVERALL LENGTH 125 mm

CAbLE LENGTH 122 cm (4 feet), including bNC connector

RESPONSE TIME 10 seconds, 90% response typical in well-stirred solution

Also see WPI’s temperature stabilzed chamber, page 88.



ISO2 Dissolved Oxygen Meter & ElectrodeOXELP Replacement Oxygen Electrode for ISO25378 Replacement Electrode Sleeve Kit (pkg of 4)

Four sleeves with membranes, plus 10 mL refill solution.7326 ISO2 Filling Solution (10 mL) 5377 Replacement ISO2 Start-up Kit

Includes T-Adapter Kit 5399, Calibration Bottle, 10mL Refill Solution, 1 cc Syringe, 2 Replacement Membranes Sleeves, MicroFil (28 ga.)

5399 T-Adapter Flow-Through KitIncludes 3 female luer T’s, 3 luer lock fittings, 3 2mm gaskets,

6 male luer to 1/8-in. tubing, 3 luer lock fittings5381 Rack Mount Kit (up to 4 units) 13347 Chart Recorder Adapter (requires bNC cable) 500184 bNC Cable



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pHOptica™ is a pH measuring system which uses fiber optic sensors and patented DLR technology . This method allows referenced measurements with single excitation to be implemented .

Features of pHOptica Meterl Single-channel, compact, easy to transport fiber-optic meter for pH

measurements with miniature sensors .

l Two 12-bit, programmable analog outputs, with electrical isolation .

l One external trigger input, with electrical isolation .

l Computer with RS232 interface required for operation .

l User-friendly software saves and visualizes measured values .

l Several pHOptica meters can be connected to one computer .

l Temperature variation is recorded using a temperature sensor .

pHOptica™ A novel fiber optic pH system

Needle-Type Housing Sensor—the glass-fiber with its pH-sensitive tip is protected inside a stainless steel needle (18 ga .); fiber has to be extended during measurement; penetration through septum .

Implantable sensor—without any housings

implantation into animal blood circuits; soil implantation; implantation in customer-made housings

Planar pH Sensitive Foils and spots—non-invasive and non-destructive measurement from outside through the wall of the flask; online monitoring .

POF Coated with a pH-Sensitive Foil—Small and robust pH dipping sensor; no reference electrode needed .

Flow-Through Cell with Integrated pH Sensor—On-line monitoring; can be easily connected via Luer-Lock adapters .

pHOPTICA SPEcIFIcaTIONSData Interface RS232Sample Rate 1 sample per secondMeasuring pH Range 5 - 9Resolution (at 20 °C) ± 0 .03 (microsensors); ± 0 .01 (minisensors)Response Time < 1 minDimensions 185 x 110 x 45 mm Weight 630 gPower Supply 100 - 220 V AC

pHOptica Micro systemThe pH Optica micro system is a single channel pH system for use with fiber optic micro sensors . The applications include:

l Penetration or implantation into living tissue (heart, muscle or animal blood vessels) .

l Soil implantation for pH measurement .

l Implantation into customer-made housing .

To protect the small glass fiber tip against breaking, suitable housings and tubings around it, depending on the respective application, were designed .

pHOptica Mini systemThe pH Optica micro system is a single channel pH system for use with fiber optic mini sensors, foil and spot surface sensors . The applications include:

l Non-invasive and non-distractive pH measurements from outside through flask walls (cell culture)

l Online pH monitoring by flow through cells

l Dipping probe pH measurements

pH mini sensorsl OD of the dipping sensor is 4 mm . l Sterilization of the pH sensor spots via gamma radiation . l The pH mini sensor meter is based on 2 mm PMMA waveguides . l Drift of 0 .1 pH units for 10,000 measurements (4 days measurement in the 30 sec data update mode) .

Two different housings and sensor spots (sensorfoils) are offered:

Features ofpH optical sensorsl No reference electrode is needed

l Immune to electrical interferences and magnetic fields

l Low drift

l High spatial resolution due to small tip size

l Measurement in very small sample volumes

l Additional optical isolation of the sensor tip is available for measurements in colored or photosynthetically active samples .

pH micro sensorsl Tip size 140 micrometer .

l Drift of 0 .1 pH units for 2000 measurements (16 hours measurement in the 30 sec data update mode) .



MINISENSOR SYSTEM (cannot be used with microsensors)PH-OPTIca-MINI Fiber Optic pH Meter for minisensors, foils and spots 502119 pH MiniTip, fiber optic pH sensors (4 mm OD), pkg of 3 502120 pH MiniFlow, fiber optic pH flow sensor, pkg of 3 502121* pH MiniFoil, fiber optic pH foil sensors, 3x3 cm2

502122* pHMiniSpot,fiberopticpHspotsensors,pkgof10,OD5mm *Requires #501644

MIcROSENSOR SYSTEM (cannot be used with minisensors)PH-OPTIca-MIcRO Fiber optic pH meter for microsensors 502123 pH MicroTip, fiber optic pH sensors (140 um OD), pkg of 3 502124 pH MicroImplant, fiber optic pH implantable sensor (140 um OD), pkg of 3 501644 Polymer optical fiber with 1 SMA connector

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Beetrode®

Micro pH Electrodeswith 100-micron sensing tips!l NEW—IMPROVED—super-miniature, superfast

coated wire pH electrodes

l 100-micron diameter — ideal for monitoring fast pH changes in very small places

BEETRODE SPEcIFIcaTIONSTIP DIAMETER 100 µ (0 .1 mm)TIP LENGTH 2 mm (approx .), except 5 mm (approx .) on NMPH5bODY DIAMETER (NMPH 1, 3, 3L, 5) 0 .187 in . (5 mm)bODY LENGTH (NMPH 1, 3, 5) 1 .875 in . (48 mm)bODY LENGTH (NMPH 3L) 0 .75 in . (19 mm)RESPONSE TIME 1 s (90%) typicalpH RANGE 0-14SLOPE NernstianRESISTANCE 100 kΩ (max)SELECTIVITY No significant interference by K+, Na+, Ca++ in 0 .1 to 1 M solutions

beetrode® is a solid state pH sensor with ideal characteristics over a wide pH range . Exhibits a larger Eo than conventional glass electrodes . Requires a separate reference electrode, such as WPI’s Dri-Ref Series. beetrodes (except the NMPH2) connect to your pH meter via a bNC-terminated cable . beetrodes generate mV readings on standard pH meters . To obtain pH-scale readings on standard pH meters, use BEE-CAL™, a small, battery-operated compensator (AA battery included) that adjusts the electrode offset potential so that beetrodes will produce standard pH-scale readings . (NMPH2B requires ZBEECAL .)

13583508

NMPH2BNMPH3

NMPH1NM

PH

5

NM

PH

3L

NMPH2

BEE-caLZBEEcaL

WPI’s pH-Silver, single/double-junction, gel-sealed Ag/AgCl combination pH electrodes are very low noise . They are designed for both laboratory and field use, especially for applications involving strong magnetic fields . The unique features of these electrodes provide unparalleled performance .

pH-SilverLow-noise combination pH electrode

l Superior stability

l Fast response

l Minimum sodium (alkaline) error

l pH 0 -14 full range measurement

PH-SILVER SPEcIFIcaTIONSbULb SHAPE ballbODY MATERIAL / COLOR Epoxy / blueTYPE OF REFERENCE Ag/AgClSEALED/REFILLAbLE Gel-SealedpH RANGE 0-14JUNCTION MATERIAL Ceramic wickJUNCTION TYPE SingleMAX . TEMPERATURE 80 °CDIMENSIONS 12 x 115 mmCAbLE LENGTH 1 .0 mCONNECTOR bNC

l Special internal fill offers full range linear temperature compensation

l Very low sensor glass membrane resistance

l zero and Isopotential: ~pH 7

l Compatible with almost all pH meters



PH-SILVER Combination pH Electrode, Low Noise

NMPH1* beetrode—2 mm receptacleNMPH2 beetrode with 1 m cable (unterminated) NMPH2-LF beetrode, Luer-Tip, with 1 m cable (unterminated) NMPH2B beetrode w/ bNC cable, 1 m cableNMPH3* Dental beetrode, 45° bend, 2 mm receptacleNMPH3L* Dental beetrode w/ 2 mm loop, 2 mm receptacleNMPH5* beetrode, 2 mm receptacleBEEcaL beetrode Offset & Cable (bNC, 2 mm pin) ZBEEcaL Level Shifting Device3508 bNC-to-US Standard Adapter1358 bNC-to-2 mm Pin Adapter

*Requires BEE-CAL or 1358 BNC-to-2 mm Pin Cable (4-ft) for connection to a pH meter.



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ISE/pH/mV/ORP/Temperature MeterUse up to 5 Standards for Calibration

Dual Channels for Multiple Analyses

Meet GLP Requirements with GLPdoc™

With dual electrode inputs, the OR720AP allows the user to select measurement from either electrode without the need for two independent meters—perfect for a busy, multi-user laboratory . Measure pH with ROSS Resolution™ to 0 .001 pH units . The pH autocalibration feature recognizes five pH buffers using built-in buffer/temperature tables . Express concentration results to three significant digits . Full five point concentration calibration with automatic blank correction improves results of low level measurements . User prompting walks you through each meter operation . Store data using the internal datalogger or print, via the RS232 port . An analog voltage output is provided for continuous recording by an external data acquisition system or chart recorder . GLPdoc records calibration parameters such as slope, temperature, mV of standards, and time and date of calibration, providing a convenient record-keeping system compatible with “Good Laboratory Practices” . Three-year meter warranty .

JE671P

OR720aP

OR320a

MODEL OR720aP OR320 JE671P

RANGE pH -2 .00 to 19 .999 0 .00 to +14 .00 00 .00 - 14 .00 mV ±1600 .0 mV — -1999 to +1999 Temp -5 .0 to 105 .0°C 0 .0 to +100 .0°C 0 .0 to +100°C Ion 0 .000 to 19900 —

RESOLUTION pH 0 .1 / 0 .01 / 0 .001 0 .1 / 0 .01 0 .01 mV 0 .1 mV — 1 mV Temp 0 .1°C 0 .1°C 0 .1°C

ACCURACY pH ±0 .002 ±0 .02 ± 0 .1 mV ±0 .05% or ±0 .2 mV — ± 0 .1% Temp ±0 .1°C ±0 .1°C ± 0 .5°C

pH MeterHard-to-find, classic pH meter JE671P allows the scientist to control all the parameters manually . Especially useful for non standard pH and ISE electrodes .

• All solid state design• Low drift and high stability• Fast input response• Manual and automatic temperature compensation• Auto polarity mV measurement

Basic Benchtop pH Meterl Digital LogR™ Temperature Compensationl Simultaneous Display of pH or mV (ORP) and Temperaturel Custom Buffer Feature Stores User Defined Buffers

OR320 PerpHecT meter gives you the benchtop pH basics for routine pH measurements plus mV (ORP) mode and selectable pH resolution (0 .01/0 .1) . Calibration is fast and accurate using the autocalibration system with built-in Auto-buffer-Recognition of three buffers . Temperature compensation can be LogR, automatic (with an ATC probe) or manual . Custom buffer option allows you to enter non-standard buffer values for accurate measurement over the entire pH range . pH electrode 9202bN included . Three-year meter warranty .



OR720aP pH/ISE Meter, 110V AC Adapter OR720BP pH/ISE Meter, 220V AC Adapter OR320a pH/ Temperature Meter, pH/Ref Electrode, 110V AC AdapterOR320B pH/ Temperature Meter, pH/Ref Electrode, 220V AC AdapterJE671P pH Meter, pH Electrode, Temperature Probe, 110V AC AdapterJE671PZ pH Meter, pH Electrode, Temperature Probe, 220V AC Adapter

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GDri-Ref™ Reference Electrodes

DRIREF-5SH

DRIREF-5

DRIREF-2

FLEXREF

SUPER-Dri-Ref, SDR2

Although the internal filling solution contains KCI, the low fluid leakage means Dri-Ref may be used in combination with ion selective electrodes, including those for K+ and Cl-, without significant contamination from the reference electrode .

The Dri-Ref electrodes are chemically resistant to strong acids and alkalines . Dri-Ref electrodes are not suitable for use in organic solvents . In addition, the long, thin FLEXREF may be easily manipulated to accommodate a difficult experimental setup .

SUPER-Dri-RefMost recently, WPI’s research staff have created an exciting new addition to the field of reference half-cell electrodes . SUPER-Dri-Ref, a reference elec-trode with a diameter of 2-mm, does not leak electrolyte at all. Exhibiting the electrical stability of a classic flowing junction reference cell, this electrode exhibits low resistance and a stable half-cell potential essentially independent of sample electrolyte concentration . SUPER-Dri-Ref is ideal for small volume and low salt concentration measurements .

Micro-Reference ElectrodeOnly 450 µm in diameter and 2 inches long, WPI’s new DRIREF-450 reference electrode can be used along with other sensors in space-restricted areas and very small sample volumes .

Luer-Tip ReferenceThe male luer fitting at the front of the DRIREF-L allows it to be easily connected to a female luer port (see WPI’s luer fittings kit, page 182) to form a tight seal — a very convenient installation for a flow-through system .

DRI-REF SPEcIFIcaTIONS DRIREF-450 DRIREF-5 DRIREF-2 FLEXREF SDR2 DRIREF-L DRIREF-5SH DRIREF-2SHLENGTH 8 cm 9 cm 13 cm 13 cm 9 cm 7 .5 cm 3 .5 cm 2 cmDIAMETER 450 µm 4 .7 mm 2 mm 1 .5 mm 2 mm Standard Luer 4 .7 mm 2 mmCONSTRUCTION Coated Glass Epoxy Isoplast™ Teflon™ PVC Polypropylene Epoxy Isoplast™LEAD LENGTH 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm) 30 in . (76 cm)CONNECTOR 2 mm pin 2 mm pin 2 mm pin 2 mm pin 2 mm pin 2 mm pin 2 mm pin 2 mm pinRESISTANCE (typical) < 5 KΩ ~500 Ω ~2 .7 KΩ ~2 .7 KΩ < 5 KΩ ~500 Ω ~500 Ω ~2 .7 KΩFILLING SOLUTION KCl KCl KCl KCl KCl KCl KCl KClELECTROLYTE LEAKAGE (ml/hr) — ~7 .4×10-7 ~5 .7×10-8 ~5 .7×10-8 — ~7 .4×10-7 ~7 .4×10-7 ~5 .7×10-8

Isoplast is a trade mark of Dow Chemical. Teflon is a trade mark of DuPont.

DRIREF-2SH

DRIREF-L

DRIREF-450

Dri-Ref™ reference electrodes were developed by WPI to have extremely low electrolyte leakage properties, hence the name “Dri-Ref” . In addition to this key feature, these electrodes exhibit stable and reproducible potential and low resistance . Stored in KCl when not in use, they have a long life expectancy . Low leakage is achieved by using KONbO™, a product com-bining modern ceramic and conductive polymer technology, as the liquid junction . Electrode resistance is low, an important consideration when mak-ing low-noise measurements .

calcium calibration SolutionscaLBUF-2For use with calcium fluorescent indicatorsCALbUF-2 is especially suitable for calibrating fluorescent Ca++ indica-tors . It provides eleven buffer standards in the 10-4 to 10-8 M Ca++ range, whereas other commonly used fluorescent Ca++ indicators have the apparent Kd in the range of 100 to 300 nM . As with any ionic sensitive indicator, the sensitivity range of these indicators is about 1 .0 log unit above and below the Kd . CALbUF-2 provides seven calibration points in this sensitivity range . It has an osmolarity of 0 .305, which is isotonic with most mammalian cells .

Concentration: 1x10-8, 4x10-8, 1x10-7, 2 .5x10-7, 5x10-7, 7 .5x10-7, 1x10-6, 4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C . Ionic strength: 0 .150 M . 11 bottles, 20 ml each . Limited shelf life; use within 30 days .

caLBUF-1For use with calcium electrodes A set of eight calcium buffers covering the range of concentration from 10-1 to 10-8 M Ca++ . Each buffer contains 20 mL of solution and enough potassium chloride to set the ionic strength to 0 .1 M . Limited shelf life; use within 30 days .

Concentration: 1x10-1, 1x10-2, 1x10-3, 1x10-4, 1x10-5, 1x10-6, 1x10-7, 1x10-8 M at 20°C . Limited shelf life; use within 30 days .



FLEXREF Flexible Dri-Ref, 1 .5 mm diam . DRIREF-2 Dri-Ref, 2 mm diam . DRIREF-2SH Dri-Ref, 2 mm diam . (Short) DRIREF-5 Dri-Ref, 4 .7 mm diam . DRIREF-5SH Dri-Ref, 4 .7 mm diam . (Short) SDR2 SUPER-Dri-Ref, 2 mm diam . DRIREF-450 Micro-Dri-Ref, 450 µm diam . DRIREF-L Reference Electrode with Luer Tip

caLBUF-1 Kit of 8 Calcium buffer SolutionscaLBUF-2 Kit of 11 Calcium buffer Solutions



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● Superior, stable PVC membrane

● Fast response

● 2mm diameter tips

● Interchangeable tip holderThese highly stable electrodes accurately measure calcium, potassium, hydrogen and TPP ion activity. Tips consist of 2 mm diameter plastic tubes sealed at one end with an ion-sensitive membrane. After filling with electrolyte solution, the user inserts the tube into the holder and connects it to a pH meter. Tips and holders are interchangeable, so one tip may be replaced with another sensitive to a different ion. Replacing a tip takes less than a minute. Electrode tips nor-mally last several months, when stored properly in saline solution. When replacement is necessary, only the tip need be replaced.

Kwik-Tip electrodes are available separately and as kits. Each “KWIK” Electrode Holder kit includes a reusable holder and three removable tips. In addition to a 4-foot BNC cable and an electrolyte filling syringe; “TIP” Electrode Kits contain three electrode tips for a specific ion. A separate reference electrode, such as WPI’s Dri-Ref™, is also required.

If your pH meter requires a US Standard connector, also order Part #3508 (BNC-to-US pH Standard adapter).

Liquid Ion Exchangers

Accurate, fast

and economical!

Each kit includes three electrode tips.

TIP ELECTRODE SPECIFICATIONS Recommended Min.PartNo. Electrode Color Filling Slope/ Concentration Selectivity Code Solution Decade Range Coefficients(-log)

TIPCA Calcium Green 0.1 M CaCl2 28 mV 0.1 M - 10-6.75 M Na+ 5.5, K+ 5.4, Mg++ 4.9

TIPH Hydrogen Orange 1 M Citric Acid, 54 mV pH 5.0 - 12 Na+ 10.4, K+ 9.8, Ca++ 11.1 0.01 M NaCl, pH 5.6

TIPK Potassium Yellow 0.1 M KCl 54 mV 0.1 M - 10-4.5 M Na+ 4.0, Ca++ 3.9, Mg++ 3.0

TIPTPP TPP+ Purple 10 mM TPP+ 54 mV 0.001 M - 10-4 M K+ 6.0

Kwik-Tip™

Ion selective electrodes

MicroFil filling syringe (included).

CATIONSION H+ K+ Ca++

CATALOG NO. IE 010 IE 190 IE 200

SELECTIVITY COEFFICIENTS*Na+ 12.7 1.97 5.5Mg++ — 2.95 4.9K+ — — 5.4Ca++ — 2.7 —USEFUL pH RANGE 2-10 4-10 4-10SLOPE 56 mV 58 mV 28 mVLINEAR RANGE pH 4-12 pK 0-3 pCa 1-7APPROX. EQUIV. — Corning ETH1001 477317*Selectivity Coefficients are expressed here as -log Kij or pKij.

When used in micropipettes to record cellular ion concentrations, consider using WPI’s Duo 773 electrometer (channel A).


World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: [email protected] • Internet:www.wpiinc.com

COMPLETE KITSKWIKCAL-2 Holder & 3 Calcium ElectrodesKWIKH-2 Holder & 3 Hydrogen ElectrodesKWIKPOT-2 Holder & 3 Potassium ElectrodesKWIKTPP-2 Holder & 3 TPP (Tetraphenylphosphonium) Electrodes

HOLDERS AND REPLACEMENT TIPSKWIK-2 Electrode Holder with BNC cableTIPCA Calcium Electrode Tips (3) TIPH Hydrogen Electrode Tips (3) TIPK Potassium Electrode Tips (3) TIPTPP TPP+ (Tetraphenylphosphonium) Electrode Tips (3)

ACCESSORIES3508 BNC-to-US pH Adapter

Also see Dri-Ref Reference Electrodes

IE010 Hydrogen Ion Exchanger (0.1 mL) IE190 Potassium Ion Exchanger (1.0 mL) IE200 Calcium Neutral Ion Exchanger (0.1 mL)

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● Rounded tip micro scissors - page 120

● Atraumatic DeBakey forceps - page 112

● Micro Olsen-Hegar needle holder - page 135

102

TweezersDumont . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103

Most.Popular.#5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104

Forceps & HemostatsCeramic-tip.forceps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108

Tissue.&.Dressing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109

Titanium. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Filter.&.Gel.Handling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115.

Hemostats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

ScissorsVannas.&.other.spring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

Dissecting.&.Surgical. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123

SuperCut. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124

Mayo.&.Metzenbaum. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125

Needle HoldersMicro.&.Castroviejo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132

Standard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133

Olsen-Hegar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135

Needles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .136

Clips & ClampsClips.&.Applying.Forceps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138

Bulldog.Clamps.&.Vessel.Clips. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138

Retractors, Knives, & CuttersRetractors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139

Sapphire.Knives. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142

Scalpel.Blades . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143

Ear.Punches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145

Rongeurs.&.Bone.Cutters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .146

Dermal.Punches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .154

Surgical Accessories & Animal Handling GlovesHooks.&.Probes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .148

Gloves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .153

Binocular.Loupes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .155

Surgery.Table. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .156

ElectrosurgeryDisposable.Cautery.Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .157

Thermal.Cautery.Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158

Electrosurgical.Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .159

Instrument CareInstrument.Protection.Tray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161

Ultrasonic.Cleaners. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .163

Dry.Sterilizer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .163

Surgical & Microdissection Tools

New in 2007


UK: Tel: 01438-880025.•.Fax: 01438-880026.•.wpiuk@wpi-europe .com.....Germany: Tel: 030-6188845.•.Fax:.030-6188670.•.wpide@wpi-europe .com



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103103

Forceps and Tweezers

Tip.Profile:

Tip.Profile:

Tip.Profile:

Tip.Profile:

Tip.Profile:

Tip.Profile:

1:1

1:1

1:1

1:1

1:1

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World Precision Instruments. •. Tel:.941-371-1003. •. Fax:.941-377-5428. •. E-mail:.sales@wpiinc .com. •. Internet: www.wpiinc.com

Dumont #1

Material:. InoxLength:. 12.cm.(4 .75.in .)Tips:. 0 .20.x.0 .12.mm.

500335. . . . . . . . . . . . . . .US$24

Dumont #1

Material:. Stainless.steel,.Medical.BiologyLength:. 12.cm.long.(4 .75.in .)Tips:. 0 .1.x.0 .06.mm.

500229. . . . . . . . . . . . . . . . US$38

Dumont #2

Material:. InoxLength:. 12.cm.(4 .75.in .)Tips:. 0 .34.x.0 .14.mm.tips

500336. . . . . . . . . . . . . . . . . . US$24

Dumont #2

Material:. Stainless.steel,.Medical.BiologyLength:. 12.cm.(4 .75.in .).Tips:. 0 .17.x.0 .07.mm

500230 . . . . . . . . . . . . . . . US$37

Dumont #3C

Material:. Dumostar,.Biology,.non-magnetic,.non-corrosiveLength:. 11.cm.(4 .3.in .).Tips:. 0 .08.x.0 .04.mm

500064 . . . . . . . . . . . . . . . US$43

Dumont #3

Material:. Dumostar,.Biology,.non-magnetic,... non-corrosiveLength:. 12.cm.(4 .75.in .)Tips:. 0 .08.x.0 .04.mm

500337. . . . . . . . . . . . . . . . US$41

Mini Dumont #M5S

Material:.. Stainless.Steel.Length:. 8 .2cm.Tips:. 0 .09.x.0 .05.mm. ..

501764 . . . . . . . . . . . . . . .US$22

Dumont #3

Material:. InoxLength:. 12.cm.(4 .75.in .)Tips:. 0 .17.x.0 .10.mm.tips

500338 . . . . . . . . . . . . . . . US$25

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MOST

POPULAR!

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Dumont #5

Material:. Dumostar,.non-magnetic,.non-corrosiveLength:. 11.cm.(4 .3.in .)Tips:. 0 .1.x.0 .06.mm.Tips

500233. . . . . . . . . . . . . . . US$44

Dumont #5

Material:. Dumoxel,.non-magnetic.stainless.steelLength:. 11.cm.(4 .3.in .)Tips:. 0 .1.x.0 .06.mm.tips

14098. . . . . . . . . . . . . . . . US$31

Dumont #5


500342 . . . . . . . . . . . . . . . US$29

Dumont #5

Material:. Inox,.BiologyLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm.tips

500341. . . . . . . . . . . . . . . US$35

Dumont #4


500340 . . . . . . . . . . . . . . . US$26

Dumont #4

Material:. Stainless.steel,.Medical.BiologyLength:. 11.cm.long.(4 .3.in .)Tips:. 0 .02.x.0 .06.mm.Mod.

500231. . . . . . . . . . . . . . . . US$44

Dumont #5Material:. Dumostar,.Medical.Biology,.. non-magnetic,.non-corrosiveLength:. 12.cm.long.(4 .75.in .)Tips:. 0 .025.x.0 .015.mm.

500085 . . . . . . . . . . . . . . . US$68

Dumont #4

Material:. Dumostar,.Biology,.non-magnetic,... non-corrosiveLength:. 11.cm.(4 .3.in .)Tips:. 0 .06.x.0 .02.mm.

500339 . . . . . . . . . . . . . . . US$46

Dumont #5


501985. . . . . . . . . . . . . . . . US$69



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Dumont #55

Material:. Inox,.BiologyLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm.

14099. . . . . . . . . . . . . . . . US$35

Dumont #5

Material:. Stainless.steel,.Medical.BiologyLength:. 11.cm.(4 .3.in .).Tips:. 0 .02.x.0 .06.mm.half.curved..

500232. . . . . . . . . . . . . . . US$46

Dumont #55

Material:. Dumostar,.Biology,.non-magnetic,.non-corrosiveLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm

500235. . . . . . . . . . . . . . . US$51

Dumont #5

Material:. Stainless.steel,.Medical.Biology,.bent.at.45°Length:. 11.cm.(4 .3.in .).Tips:. 0 .05.x.0 .01.mm.

14101 . . . . . . . . . . . . . . . . US$45

Dumont #5

Material:. Stainless.steel,.Medical.BiologyLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm.

14095. . . . . . . . . . . . . . . . . US$41

Dumont #5

Material:. Dumoxel,.self-closingLength:. 11.cm.(4 .3.in .)Tips:. 0 .1.x.0 .06.mm.

501203. . . . . . . . . . . . . . . US$39

Dumont #5

Material:. Inox,.Biology,.self-closingLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm.

501202. . . . . . . . . . . . . . . US$37

Dumont #5

Material:. Medical,.90°.bentLength:. 11.cm.(4 .3.in .)Tips:. 0 .05.x.0 .01.mm.

501201. . . . . . . . . . . . . . . . US$41

Dumont #5

Material:. TitaniumLength:. 11.cm.(4 .3.in .)Tips:. 0 .1.x.0 .06.mm.

14096. . . . . . . . . . . . . . . . US$38

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Protect your valuable instruments with a sterilizer/storage tray

For more information, see page 161



Dumont #7

Material:. Dumoxel,.Biology,.self-closingLength:. 12.cm.(4 .75.in .)Tips:. 0 .08.x.0 .04.mm

501206. . . . . . . . . . . . . . . US$44

Dumont #7

Material:. Inox,.Biology,.self-closing,Length:. 12.cm.(4 .75.in .)Tips:. 0 .07.x.0 .03.mm,.curved

501205. . . . . . . . . . . . . . . US$37

Dumont #7

Material:. stainless.steelLength:. 11.cm.(4 .3.in .),.curved,.serrated.jawsTips:. 0 .17.x.0 .10.mm

501302. . . . . . . . . . . . . . . US$56

Dumont #5B

Material:. Inox,.Biology,.bent.at.45°Length:. 11.cm.(44 .3.in .)Tips:. .0 .05.x.0 .01.mm.

500234 . . . . . . . . . . . . . . US$39

Dumont #7

Material:. Dumoxel,.non-magnetic... stainless.steelLength:. 12.cm.(4 .75.in .)Tips:. 0 .17.x.0 .1.mm

14188 . . . . . . . . . . . . . . . . US$39

Dumont #7

Material:. InoxLength:. 12.cm.(4 .75.in .)Tips:. 0 .17.x.0 .1.mm

14097. . . . . . . . . . . . . . . . . US$32

Dumont #6

Material:. Stainless.Steel,.MedicalLength:. 12.cm.(4 .75.in .)..sharp.pointsTips:. 0 .17.x.0 .1.mm

501204. . . . . . . . . . . . . . . US$38

500252. Sterilizer.tray,.15.x.6.x.2.cm.(6.x.2 .5.x.0 .75.in .),.base,.silicone.mat,.lid. US$35500253. Sterilizer.tray,.19.x.10.x.2.cm.(7 .5.x.4.x.0 .75.in .),.base,.silicone.mat,.lid. US$60500254. Sterilizer.tray,.25 .5.x.15.x.2.cm.(10.x.6.x.0 .75.in .),.base,.silicone.mat,.lid. US$90501728 Sterilizer.Tray,.25 .5.x.15.x.4.cm.(10.x.6.x.1 .5.in .),.base,.insert.tray,.lid,.2.mats. US$207501729 Sterilizer.Tray.for.Knife.15.x.6.x.2.cm.(6.x.2 .5.x.0 .75.in .),.base,.lid,.2.bars. US$35 500255. Silicone.mat,.38.x.25 .5.cm.(15.x.10.in .). US$83

Dumont #7

Material:. TitaniumLength:. 12.cm.(4 .75.in .)Tips:. .0 .17.x.0 .1.mm.

14187 . . . . . . . . . . . . . . . . US$40



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Economy Fine Tweezers Made.of.quality.stainless.steel,.these.tweezers.are.useful.for.general.dissections.where.ultrafine.tips.are.not.necessary ..Economically.priced.per.

package.of.6,.they.are.affordable.enough.for.everyone.to.have.their.own.instruments .

All tweezers on this page are sold in packages of 6

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Economy Tweezer Style 1

Material:. Stainless.SteelLength:. 11.cm.(4 .3.in .)Tips:. 0 .45.x.0 .55.mm.

501974-6. . . . . . . . . . . . . . US$46



501975-6. . . . . . . . . . . . . . US$46



501976-6. . . . . . . . . . . . . . US$46

Economy Tweezer Style 3C


501977-6. . . . . . . . . . . . . . US$46



501978-6. . . . . . . . . . . . . . US$46



501979-6. . . . . . . . . . . . . . US$46



501980-6. . . . . . . . . . . . . . US$46



501981-6. . . . . . . . . . . . . . US$46

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Ceramic-tipped Forceps Zirconia.ceramic.forceps.have.several.unique.properties.that.are.not.present.in.metal.forceps ..● Ceramic.contains.no.metal,.therefore,.the.forceps.will.not.leave.any.trace.metal.residue.on.tissue ..This.is.especially.useful.in.forensic.science..

research .● Ceramic.is.not.electrically.conductive.so.it.can.be.used.for.handling.gels.in.an.electrophoresis.bath .● Ceramic’s.corrosion.resistance.allows.its.use.in.highly.corrosive.solutions ..For.example,.clean.quarz.cover.glass.or.lithographic.produced.chip..

sensors.in.an.acid.solution.without.damaging.the.forceps .● Ceramic’s.low.heat.conductivity.and.high.heat.resistance.allow.it.to.hold.parts.in.a.flame.for.sterilizing.or.fabricating ..Although.the.flame.will.

eventually.reduce.the.strength.of.the.ceramic.tip,.it.will.be.minimal .

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Titanium Forceps Titanium.is.100%.non-corrosive,.non-magnetic.and.has.a.heat.resistance.to.550°C ..More.flexible.than.stainless.steel,.the.tips.are.more.likely.to.bend.under.pressure.than.break ..

* Part numbers ending in “-G” indicate German-made instruments Titanium instruments MRI compatible



Dumont #3C

Material:. DumoxelLength:. 11.cm.(4 .3.in .)Tips:. 0 .4.x.0 .3.mm.Ceramic.

501209. . . . . . . . . . . . . . . . US$62

Dumont #3

Material:. DumoxelLength:. 13 .5.cm.(5 .3.in .)Tips:. 0 .4x.0 .3.mm.Ceramic.

501210. . . . . . . . . . . . . . . . US$60

Dumont #3

Material:. Dumoxel,.self-closingLength:. 12.cm.(4 .75.in .)Tips:. 0 .4.x.0 .3.mm.Ceramic.

501208. . . . . . . . . . . . . . . . US$66

Zirconia.Ceramic.Forceps.with.plastic.handles,.14.cm.(5 .5.in .).long,..9.mm.straight.tips

501207. . . . . . . . . . . . . . . US$45

Tweezer, Titanium,.“#7.Biology”.style,.12.cm.(4 .75.in .).long,.0 .07.x.0 .03.mm.tips

555230F . . . . . . . . . . . . . US$64

Tweezer, Titanium,.“#5.45°.Biology”.style,.11.cm.(4 .3.in .).long,.0 .05.x.0 .01.mm.tips.bent.at.45°

555229F. . . . . . . . . . . . . . US$64

Tweezer,.Titanium,.“#5.Biology”.style,.11.cm.(4 .3.in .).long,.0 .06.x.0 .01.mm.tips

555227F. . . . . . . . . . . . . . US$64



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* Part numbers ending in “-G” indicate German-made instruments

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Titanium

Titanium

Titanium



Graefe Forceps, 1 x 2 teeth

7.cm.(2 .75.in .).long,.1x2.teeth,.curved.0 .7.mm.tips

14141 . . . . . . . . . . . US$21 14141-G*. . . . . . . . . US$75

Graefe Forceps, 1 x 2 teeth

.7.cm.(2 .75.in .).long,.straight.0 .7.mm.tips

14140 . . . . . . . . . . . . US$21 14140-G* . . . . . . . . . US$75

Graefe forceps, serrated

7cm.(2 .75.in .).long,.straight..0 .7.mm.tips14142 . . . . . . . . . . . . . US$21 14142-G* . . . . . . . . . . US$59

7cm.long,.curved..0 .7.mm.tips501215 . . . . . . . . . . . . US$21 501215-G* . . . . . . . . . US$59

Bishop Harmon forceps, serrated,

9.cm..(3 .5.in .).long

501775 . . . . . . . . . . US$23

501775-G* . . . . . . US$174

Iris forceps, serrated

10.cm.(4.in .).long,.curved.0 .8.mm.tips

15915 . . . . . . . . . . . . US$17 15915-G* . . . . . . . . . . US$35

Iris forceps, serrated

10.cm.(4.in .).long,.straight.0 .8.mm.tips

15914 . . . . . . . . . . . . .US$17 15914-G* . . . . . . . . . . US$35

Bishop Harmon forceps, 1 x 2 teeth

9.cm..(3 .5.in .).long

501776 . . . . . . . . . US$23

501776-G* . . . . . US$210

555053FT . . . . . US$299

Iris Forceps, 1 x 2 teeth

10.cm.(4.in .).long,..straight,.0 .8.mm.tips

15916 . . . . . . . . . . . . US$17 15916-G* . . . . . . . . . US$35

555054FT . . . . . . .US$276

Iris Forceps, 1 x 2 teeth

10.cm.(4.in .).long,..curved.0 .8.mm.tips

15917 . . . . . . . . US$17 15917-G* . . . . . .US$37

555056FT . . US$276

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Tungsten Carbide

Tungsten Carbide

Tungsten Carbide



Adson Forceps, serrated12.cm.(4 .75.in .).long,..............................jaws

500222. . . . . . .US$37

500222-G*. . .US$114

Adson Forceps, serrated12.cm.(4 .75.in .).long

14226. . . . . . . . . . US$17

14226-G*. . . . . . .US$33

Adson Forceps, 1 x 2 teeth 12.cm.(4 .75.in .).long.............................jaws.

500178 . . . . . . . . . US$37

500178-G* . . . . .US$104

Adson-Brown Forceps12.cm.(4 .75.in .).long,..jaws.with.7x7.side.grasping.teeth

500177 . . . . . . . . . US$39

500177-G* . . . . . US$114

Tissue Forceps

12 .5.cm.(5.in .).long,.1.x.2.teeth15918 . . . . . . US$17 15918-G* . . . . . . US$29

12 .5.cm.(5.in .).long,.curved,.1.x.2.teeth501216 . . . . . US$18 501216-G* . . . . . US$29

15.cm.(6.in .).long,.straight,.1.x.2.teeth501742-G*. . . . . . . . . . . . . US$29

15.cm.(6.in .).long,.straight,.2.x.3.teeth501741-G* . . . . . . . . . . . . US$29

Micro Adson Dressing Forceps, serratedfine.point,.12.cm.(4 .75.in .).long

501245. . . . . . . . . US$25

501245-G*. . . . . . US$48

Micro Adson Tissue Forceps, 1 x 2 teethfine.point,.12.cm.(4 .75.in .).long,.0 .8.mm.tips

501244. . . . . . . . . US$25

501244-G*. . . . . . US$48

Adson Forceps, 1 x 2 teeth 12.cm.(4 .75.in .).long,.

500092 . . . . . . . . US$18

500092-G*. . . . . . US$37



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Tungsten Carbide

Tungsten Carbide



Gillies Dissecting Forceps, 1 x 2 teeth

15 .5.cm.(6.in .).long

501266. . . . . . .US$22

501266-G*. . . US$114

15 .5.cm.(6.in .).long,...............................jaws.

501265. . . . . . .US$45

501265-G*. . . US$114

McIndoe Dressing Forceps, serrated15 .5.cm.(6.in .).long.

501268. . . . . . .US$22

501268-G*. . . US$114

15 .5.cm.(6.in .).long,..............................jaws.

501267. . . . . . .US$45

501267-G*. . . US$114

Dressing Forceps, serrated

15 .5.cm.(6.in .).long.(pictured)500363 . . . . . . . . . US$18 500363-G* . . .US$35

25 .5.cm.(10.in .).long500364 . . . . . . . . . US$18 500364-G* . . .US$35

30 .5.cm.(12.in .).long500365 . . . . . . . . . US$18 500365-G* . . .US$35

12 .5.cm.(5.in .).long501217 . . . . . . . . . . US$18 501217-G*. . . .US$35

Dressing Forceps, ultra fine, serrated 12 .5.cm.(5.in .),.straight,.sharp.tips

15908. . . . . . . . . . .US$24

Micro Tweezer

15.cm.(6.in .),.serrated,.angled

501727-G* . . . . . . .US$40

Graefe Fixation Forceps

Length:. 11 .5cm.(4 .5.in .)Tips:. 4mm.wide,.5x5.teeth.

501988. . . . . . .US$30

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Special cupped forceps allow atraumatic gripping of organs and other delicate tissue.

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Atraumatic.teeth.grip.tissue,.vessels,.and.organs.without..damage .



Teflon-Coated Cupped Forceps

Length:. 15 .25.cm.(6.in .)Tips:. 3 .5mm.diameter.

501983. . . . . . .US$86

Russian Forceps

Length:. 15 .25cm.(6.in .)Tips:. serrated,.oval,.7mm.diameter.

501987. . . . . . .US$33

DeBakey Forceps

15.cm.(6.in .).long,.delicate,.jaw.width.1 .5.mm

501239. . . . . . .US$69501239-G*. . . US$114

45.degree.angled.tip

501996. . . . . . .US$69

16.cm.(6 .25.in .).long,.angled,.micro,.jaw.width.1 .2.mm

503212. . . . . .US$154

16.cm.(6 .25.in .).long,.micro,.jaw.width.1 .2.mm

503213. . . . . .US$155



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Round-handled Forceps By.hollowing.out.the.handles.of.long,.round-handled.instruments,.the.overall.weight.is.decreased ..This.makes.them.efficient.and.non-fatiguing.when.operating.under.a.microscope ..Made.from.high.quality.stainless.steel,.these.instruments.are.an.excellent.addition.to.a.microsurgical.instrument.kit .

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Titanium instruments ● 100%.non-corrosive.(great.for.seawater.procedures). ● Forcep.tips.coated.with.tungsten.carbide.for.increased.gripping.power● 100%.non-magnetic.(perfect.for.MRI). . . ● Anodized,.non-glare.blue.finish.● 40%.lighter.than.stainless.steel.(reduces.hand.fatigue)



ForcepsLength:. 8 .5.cm.(3 .3.in .).Tips:. straight,.notched.

555081FT . . . US$212

ForcepsLength:. 8 .5.cm.(3 .3.in .)Tips:. straight.with.0 .12.mm.teeth.(1x2)

555041FT . . . US$216

ForcepsLength:. 8 .5.cm.(3 .3.in .)Tips:. straight.with.tying.platforms

555001FT . . .US$207

Round, Hollow Handle ForcepsLength:. 15.cm.(6.in .)Tips:. 0 .15.mm.tips.. bent.at.45°

14209. . . . . . . US$114

Round, Hollow Handle Forceps

Length:. 15.cm.(6.in .)Tips:. straight.0 .15.mm.

14113 . . . . . . . .US$8714113-G* . . . US$263

Round, Hollow Handle Forceps

Length: 15.cm.(6.in .)Tips:. curved.0 .3.mm.tips

14114 . . . . . . . .US$8714114-G* . . . US$263

Dilator ForcepsLength:. 14.cm.(5 .5.in .)Tips:. straight.0 .15.mm.tips

15910 . . . . . . . .US$84

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.555827L . . . . . . . US$2762mm.OD.rings108mm.long

.555828L . . . . . . . US$2761mm.OD.flat.oval.tips108mm.long

Kelman-McPherson ForcepsLength:. 8 .5.cm.(3 .25.in .)Tips:. 45º.angled,.7 .5.mm.long.smooth.platform.

555190FT. .. .. US$244

Colibri Suturing Fine ForcepsLength:. 7 .5.cm.(3.in .)Tips:. 140º.toothed.(1×2.teeth),.0 .1.mm. with.tying.platform.

555060FT . . US$258.

90° Toothed Forceps.

Length:. 11 .5.cm.(4 .5.in .)Tips:. 0 .12mm.1x2.teeth,.straight,.. .. with.tying.platform.

555047FT . . US$258

Notched forceps,.standard.range,

Length:. 8 .5.cm.(3 .25.in .)Tips:. straight,.1×1.teeth,.with... tying.platform.

555080FT . . .US$193

Suturing Forceps,.ultrafine.range,

Length:. .11 .5.cm.(4 .5.in .)Tips:. straight,.140º.toothed.(1x2.teeth),... 0 .12.mm,.with.tying.platform.

555063FT . . US$258

Tying Forceps,.Standard.range,.

Length:. 11 .5.cm.(4 .5.in .)Tips:. straight,.0 .25.x.0 .15.mm.tips,.... with.4.mm.long.tying.platform.

555007FT . . .US$193.

Colibri ForcepsLength:. 8 .5.cm.(3 .3.in .)Tips:. curved.with.0 .12.mm.teeth.(1x2)

555042FT . . .US$221



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This.micro.cannula.is.ideal.for.placement.in.the.carotid.or.femoral.artery.of.mice,.rats,.and.other.small.animal.blood.vessels ..It.can.be.used.with.a.pressure.transducer.(WPI’S.BLPR).for.blood.pressure.measurement,.or.in.conjunc-tion.with.a.micro-syringe.injection.system.(like.WPI’s.UMPII.or.MMP.pumps) ..The.incorporated.standard.female.luer.fitting.makes.connecting.to.existing.experimen-tal.plumbing.quick.and.easy ..The.cannula.is.provided.with.a.contoured-tip.stainless.steel.stylet.(trocar).to.facilitate.placement.using.established.techniques ..A.movable.“shoulder”.ring.provides.a.tie-in.point.to.prevent.accidental.removal ..The.cannula.may.be.left.in.place.for.2.hours.or.more,.and.with.proper.care.and.cleaning,.may.be.re-used.multiple.times ..Instructions.for.use.included .

Great for gel handling

Tip.Profile:1:1

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Tip.Profile:

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See Mouse Vessel Cannulation Kit on page 152

Micro Cannula

l.0.4mm O.D., 0.2mm I.D. tubingl.Autoclavablel.Biocompatible Perfluorocarbon tubing material



Filter Forceps11.cm.(4 .3.in .).long,.straight.flat.jaw

500456 . . . . . .US$22 500456-G* . . . US$35

Microintraocular forcepsLength:. 14.cm.(5 .5.in .)Jaws:. 2 .5.x.0 .3.x.0 .25mm.(lxwxt),.1 .5mm.maximum.opening

500373. . . . . US$299

500373-T. . . US$345

Length:. 14.cm.(5 .5.in .)Jaws:. 3.x.0 .6.x.0 .4mm.(lxwxt),.1 .4mm.maximum.opening

501212 . . . . . US$299

501212-T . . . US$345

Filter ForcepsPolypropylene.finger.grip,.11.cm.(4 .3.in .).long,.straight.flat.jaw

500455 . . . . . .US$22

Vessel Cannulation Forceps.for.0 .5mm.diameter.cannula,.10.cm.(4.in .).long

500377. . . . . . .US$79

Vessel Cannulation forceps.for.1 .0.mm.diameter.cannula,.10.cm.(4.in .).long

500453 . . . . . .US$79

Notched Forceps,.standard.range,

Length:. 11 .5.cm.(4 .5.in .)Tips:. 1×1.teeth,.with.tying... platform,.straight

555084FT . . .US$239

KZ1101. Micro.Cannula,.3". US$92

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Titanium

Titanium

1:1

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Mosquito Hemostatic Forceps15920 12 .5.cm.(5.in .).long,.straight . . . . . US$17 15920-G*. . . . . . . . US$35

15921 12 .5.cm.(5.in .).long,.curved. . . . . . US$17 15921-G*. . . . . . . . US$35

501705 9.cm.(3 .5.in .).long,.straight . . . . . . US$17 501705-G*. . . . . . . US$35

501291 9.cm.(3 .5.in .).long,.curved. . . . . . . US$17 501291-G*. . . . . . . US$35

555222F 9.cm.(3 .5.in .).long,.straight . . . . . US$230

555223F 9.cm.(3 .5.in .).long,.cuved . . . . . . US$230

Micro Hemostatic Forceps

12 .5.cm.(5.in .).long,.straight,.serrated,.0 .4.x.0 .8.mm.tips

500451. . . . . . . US$39 500451-G* . . . . . . . .US$51

12 .5.cm.(5.in .).long,.curved,.serrated,.0 .4.x.0 .8.mm.tips

500452 . . . . . . US$39 500452-G*. . . . . . . .US$52

Filter Forceps11.cm.(4 .3.in .).long,.bent.flat.jaw

500457. . . . . . .US$22

Wafer Handling Forceps11.cm.(4 .3.in .).long,.angled.30°.jaw

501303. . . . . . .US$45

Backhaus Towel Clamp

3.5” 501320. . . . . US$17 501320-G* . . . . . US$35 .

5.25” 501700. . . . . US$18. 501700-G* . . . . . US$37

.



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Crile Hemostatic Forceps501242 Straight,.14.cm.(5 .5.in .).long . . . . US$18 501242-G*. . . . . . .US$37

501730 Curved,.14.cm.(5 .5.in .).long. . . . . US$18 501730-G*. . . . . . .US$37

501712 Straight,.15 .5.cm.(6.in .).long . . . . US$20 501712-G* . . . . . . .US$39

501713 Curved,.15 .5.cm.(6.in .).long. . . . . US$20 501713-G* . . . . . . .US$39

Kelly Hemostatic Forceps501241 Straight,.14.cm.(5 .5.in .).long. . . . .US$18 501241-G* . . . . . . US$37

501288 Curved,.14.cm.(5 .5.in .).long.. . . . .US$18 501288-G* . . . . . . US$37

501714 Straight,.15 .5.cm.(6.in .).long. . . . .US$20 501714-G*. . . . . . . US$39

501715 Curved,.15 .5.cm.(6.in .).long . . . . .US$20 501715-G*. . . . . . . US$40

Rochester-Oschner Hemostatic Forceps501709 Straight,.14.cm.(5 .5.in .).long. . . . . . .US$22 501709-G* . . . . . . . . .US$35

501710 Curved,.14.cm.(5 .5.in .).long . . . . . . .US$22 501710-G* . . . . . . . . .US$35

501326 Straight,.16.cm.(6 .25.in .).long . . . . .US$23 501326-G*. . . . . . . . .US$37

501711 Curved,.16.cm.(6 .25.in .).long . . . . . .US$23 501711-G* . . . . . . . . .US$37

Allis Tissue Forceps6”,.4x5.teeth

501324. . . . . . . . . . US$37

501324-G*. . . . . . . US$74

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8.cm

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Micro Alligator Forceps8.cm.(3.in .).shaft,.0 .5.x.4 .5.mm.serrated.jaw

500446 . . . . . . . . US$242 500446-G*. . . . . . . US$403

Micro Crocodile Forceps7 .5.cm.(3.in .).shaft,.3.mm.serrated.jaw

501211 . . . . . . . . . US$82

Rochester-Carmalt Hemostatic Forceps501243 Curved,.16.cm.(6 .25.in .).long . . . US$23 501243-G*. . . . . . . . US$46

501287 Straight,.16.cm.(6 .25.in .).long. . . US$23 501287-G*. . . . . . . . US$46

501716 Curved,.20.cm.(7 .75.in .).long . . . US$29 501716-G* . . . . . . . . US$52

501717 Straight,.20.cm.(7 .75.in .).long. . . US$29 501717-G* . . . . . . . . US$52

Rochester-Pean Hemostatic Forceps501325 Straight,.16.cm.(6 .25.in .).long . . . . . US$21 501325-G*. . . . . . . US$37

501706 Curved,.16.cm.(6 .25.in .).long. . . . . . US$21 501706-G*. . . . . . . US$37

501707 Straight,.19.cm.(7 .5.in .).long. . . . . . . US$23 501707-G* . . . . . . . US$38

501708 Curved,.19.cm.(7 .5.in .).long . . . . . . . US$23 501708-G*. . . . . . . US$38

Mixter Hemostatic Forceps

.“Baby”,.right.angle,.14.cm.(5 .5.in .).long

501240. . . . . . . . . .US$33

501240-G*. . . . . . .US$69



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Scissors

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McPherson-Vannas Scissors. Length:. 7.cm.(2 .75.in .). Blades:. straight.3.mm..Tips:. 0 .1mm

.Length:. 7.cm.(2 .75.in .)

. Blades:. curved.3.mm.

.Tips:. 0 .1mm.Tip.Profile:

1:1

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Tip.Profile:

Tip.Profile:

Finest Tips in the World!

1:1



Vannas ScissorsLength:. 8.cm.(3.in .)Blades:. 45°.angled.to.side,.5.mm.Tips:. 0 .1.mm.tips

500260 . . . . .US$148. 500260-G*. . . . US$228

Vannas ScissorsLength:. 8 .5.cm.(3 .3.in .).Blades:. straight.7mm.. 500086. . . . . . . . . . US$183Tips:. 0 .025.x.0 .015.mm. .Length:. 8 .5.cm.(3 .3.in .)Blades:. curved.7mm.. 501232 . . . . . . . . . . US$190Tips:. 0 .025.x.0 .015.mm.

.

Vannas ScissorsLength:. 8.cm.(3.in .).Blades:. straight.5.mm.Tips:. 0 .1.mm.tips

14003. . . . . . .US$132 14003-G* . . . . . .US$213

Vannas Scissors Length:. 8.cm.(3.in .)Blades:. curved,.5.mmTips:. 0 .1.mm.tips

14122. . . . . . . US$137 14122-G* . . . . . US$229

14177 . . . . . . .US$146

501233. . . . . .US$153

Vannas Scissors, Super FineLength:.8.cm..Blades:.straight.3.mm. 501778 . . US$194. .Tips:.0 .015.x.0 .015.mm.

Length:.8.cm..Blades:.curved.3.mm. 501839 . . US$209. .Tips:.0 .015.x.0 .015.mm.

Vannas Scissors, angled on flat Length:. 8.cm.(3.in .)Blades:. angled,.5.mmTips:. 0 .1.mm.tips

501790. . . . . . US$137

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McPherson-Vannas Scissors.Length:. 8.cm.(3.in .).Blades:. straight.5.mm..Tips:. 0 .1mm

.Length:. 8.cm.(3.in .)

.Blades:. curved.5.mm.

.Tips:. 0 .1mm.

Tip.Profile:

Tip.Profile:

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14124 . . . . . . .US$132 14124-G*. . . . . . .US$263

501234. . . . . .US$137 501234-G* . . . . US$268

Vannas Scissors,.Titanium

Length:. 8.cm.(3.in .)Blades:. straight.3.mm.

14364. . . . . . US$230


Length:. 8.cm.(3.in .)Blades:. straight.7 .5.mm.

555640S . . . US$230


Length:. 8.cm.(3.in .)Blades:. angled.3.mm

14365. . . . . . US$230

McPherson-Vannas ScissorsLength:. 8.cm.(3.in .)Blades:. sraight,.3.mm.Tips:. 0 .22.mm.rounded.tips

503215. . . . . . US$114.

McPherson-Vannas ScissorsLength:. 8.cm.(3.in .)Blades:. curved,.5.mm.Tips:. 0 .3.mm.rounded.tips

503217 . . . . . . US$114.

McPherson-Vannas ScissorsLength:. 8.cm.(3.in .)Blades:. angled.on.flat,.5.mm.Tips:. 0 .5.mm.rounded.tips

503216. . . . . . US$114.

McPherson-Vannas ScissorsLength:. 8.cm.(3.in .)Blades:. 45.angled.to.side,.5.mm.Tips:. 0 .22.mm.rounded.tips

503214. . . . . . US$114.



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Length:. 8.cm.(3.in .)Blades:. angled.7 .5.mm.

555641S. . . . US$230

Vannas Scissors, TitaniumLength:. 11.cm.(4 .3.in .)Blades:. straight,.sharp.tips,.10 .5.mm.

555580S . . . .US$271

Iris Scissors, TitaniumLength:. 10 .5.cm.(4.in .)Blades:. curved

555561S. . . . . US$216


Length:. 10 .5.cm.(4.in .)Blades:. curved,.sharp.tips,.12 .5.mm.

555583S . . . US$294

Length:. 10 .5.cm.(4.in .)Blades:. curved,.sharp.tips,.10 .5.mm

555582S . . . .US$271

Westcott Stitch Scissor, Titaniums

Length:. 11.cm.(4 .3.in .).

Blades:. very.sharp.pointed.tips

555541S. . . . US$244

Vannas Scissors, Titanium

Length:. 10 .5.cm.(4.in .)Blades:. angled,.sharp.tips,.10 .5.mm

555584S . . . .US$271

Castroviejo Corneal Scissors, Titanium

Length:. 12.cm..(4 .75.in .)Blades:. curved,.blunt.tips,.cuts..6/0.traction... suture,.round.handles.

555526S . . . .US$271

McPherson-Westcott scissors, Titanium

Length:. 11.cm.(4 .3.in .)Blades:. curved.blunt.tips,.cuts.4-0.suture

555500S . . . . US$212

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Spring Scissors.Length:. 10 .5.cm.(4.in .).Blades:. straight.8.mm.

.Length:. 10 .5.cm.(4.in .)

.Blades:. curved.8.mm.

Tip.Profile: 1:1

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Tip.Profile:

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Spring ScissorsLength:. 12.cm.(4 .75.in .)Blades:. straight.12.mm.extra.fine.and.long

14125 . . . . . . .US$148

Length:. 12.cm.(4 .75.in .)Blades:. curved.12.mm.extra.fine.and.long

14126 . . . . . . .US$148

Noyes Scissors, sharp/sharp tips

500228 12.cm.long,.straight.15.mm.blades. . . .US$49 500228-G*. .. .. . US$229501236 12.cm.long,.curved.15.mm.blades . . . .US$49.. 501236-G*. . . . US$207

501237 14.cm.long,.straight.20.mm.blades. . . .US$49 501238 14.cm.long,.curved.20.mm.blades . . . .US$49..

Spring Scissors, round handesLength:. 14.cm.(5 .5.in .)Blades:. straight.6 .5.mm.

14111 . . . . . . . . US$91

14111-G* . . . US$357

Spring Scissors, round handlesLength:. 14.cm.(5 .5.in .)Blades:. curved.7.mm.

14112 . . . . . . . . US$91

14112-G* . . . .US$361

501235. . . . . .US$102

14127 . . . . . . .US$102 14127-G*. . . US$252

WestcottTenotomy Scissors, Titanium

Length:. 11 .5.cm.(4 .5.in .)Blades:. curved.blunt.tips

555620S . . . US$244

Conjunctival Scissors, Titanium

Length:. 12.cm.(4 .75.in .)Blades:. curved,.blunt.tips

555501S. . . . US$258



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Dissecting Scissors

1:1

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1:1

2 .2.cm

2 .2.cm

2 .3.cm



Spring ScissorsLength:. 14.cm.(5 .5.in .)Blades:. straight.8.mm.

15905. . . . . . . .US$60

15905-G*. . . .US$276

Length:. 14.cm.(5 .5.in .)Blades:. curved.8.mm.

15906. . . . . . . .US$60

15906-G*. . . .US$276

14393 10.cm.(4.in .).straight . . . . US$17 14393-G*. . . . . . US$40

14394 10.cm.(4.in .).curved. . . . . US$17 14394-G*. . . . . . US$41

15922 12 .5.cm.(5.in .).straight . . US$17 15922-G*. . . . . . US$35

15923 12 .5.cm.(5.in .).curved . . . US$17 15923-G*. . . . . . US$35

Iris Scissors

Length:. 11.cm.(4 .3.in .)Blades:. .straight..

501758. . . . . . .US$22 501758-G* . . . . .US$45

Length:. 11.cm.(4 .3.in .)Blades:. curved.

501759. . . . . . .US$22 501759-G* . . . . . US$45

Length:. 11.cm.(4 .3.in .)Blades:. curved,.Tungsten.Carbide.

500217 . . . . . . . US$51 500217-G*. . . . . US$102

Iris Scissors

Length:. 11.cm.(4 .3.in .)Blades:. .straight,.Tungsten.Carbide..

500216. . . . . . . US$51. 500216-G* . . . . .US$102

Spring Scissors, SuperCutone edge micro serrated, one edge honed to the sharpness of a knife edge

Length:. 12.cm.(5.in .)Blades:. straight..9mm.

501924. . . . . . US$217

Length:. 12.cm.(5.in .)Blades:. curved..9mm.

501925. . . . . . US$217

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Ribbon Handle Iris ScissorsLarger ring handles for ease of use

1:1

Iris Scissors, SuperCut Tungsten CarbideThese.are.the.finest.scissors.available ..They.combine.the.precise.cutting.action.of.the.razor-sharp.

SuperCut.blades.with.the.durability.of. Tungsten Carbide ..They.will.provide.a.clean,.smooth.cut.

and.retain.their.sharpness.for.an.extended.period.of.time .

2 .3.cm

2 .3.cm

3.cm

2 .5.cm

2 .5.cm

Shown: 10 cm, straight

Shown: 12.5 cm, straight



Utility Eye Scissors, Titanium

Length:. 11.cm.(4 .3.in .).Blades:. straight,.standard.pointed.tips

555646S . . . .US$221

501767.

11.cm.(4 .3.in .).long

Straight 501767.. . . . . . . .US$45

Curved 501768. . . . . . . . .US$45

11.cm.(4 .3.in .).long

Straight. 501263. . . . . . . . . . . . . . . . .US$98

501263-G*. . . . . . . . .US$194

Curved. 501264. . . . . . . . . . . . . . . . .US$98

501264-G*. . . . . . . . .US$197

Iris Scissors, SuperCut one edge micro serrated, one edge honed to the sharpness of a knife edge

12 .5.cm.(5.in .)

Straight. 14225. . . . . . . . . .US$51 14225-G*. . . . . . . . . . .US$75

Curved. 14224. . . . . . . . . .US$51 14224-G*. . . . . . . . . . .US$75

14.cm.(5 .5.in .)

Straight. 503261. . . . . . . . . .$68

Curved. 503262. . . . . . .US$74

Iris Scissors, SuperCut one edge micro serrated, one edge honed to the sharpness of a knife edge

10.cm.(4.in .)

Straight. 14218 . . . . US$51 14218-G*. . . . . .US$75

Curved. 14219 . . . . US$51 14219-G*. . . . . .US$75

11 .5.cm.(4 .5.in .)

Straight. 503259 . . US$56

Curved. 503260 . . US$62.



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3.5 cm

Metzenbaum Scissors

1:1

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Metzenbaum Scissors, Carbide Blades

1:1

Tip Profile:

1:1

1:11.3 cm

1 cm

3.5 cm

3 cm

Shown: 10 cm, straight


Carbide is a harder metal than stainless steel and will retain its sharpness longer

one edge micro serrated, one edge honed to the sharpness of a knife edge


1:1



Tenotomy Scissors, SuperCut one edge micro serrated, one edge honed to the sharpness of a knife edge

10 cm (4 in.) Straight 14395 ......US$51 14395-G* ......US$75

Curved 14396 ......US$51 14396-G* ......US$75

11.5 cm (4.5 in.) Straight 503263 ...US$56

Curved 503264 ...US$62

12.5 cm (5 in.), Straight 14220 ...... US$51 14220-G* ....... US$75

Curved 14221 ....... US$51 14221-G*........ US$75

14 cm (5.5 in.) Straight 503265 ...US$68

Curved 503266 ...US$74

16 cm (6.2 in.) Straight 503267 ...US$78

Curved 503268 ...US$84

11.5 cm (4.5 in.) Straight 501747 ..... US$17 501747-G* ........ US$37

Curved 501748 ..... US$17 501748-G* ....... US$37

14.5 cm (5.75 in.) Straight 501252 ..... US$17 501252-G* ....... US$37

Curved 501253 ..... US$17 501253-G* ....... US$37

17.5 cm (7 in.) Straight 501254 ...... US$18 501254-G* ..........US$40

Curved 501255 ........US$18 501255-G* ..........US$40

11.5 cm (4.5 in.) Straight 501739 ...... US$58 501739-G* . . . US$114 Curved 501740 ...... US$58 501740-G* . . . US$114

14.5 cm (5.75 in.) Straight 501733 ...... US$58 501733-G* . . . US$114

Curved 501734 ...... US$60 501734-G* . . . US$114

18 cm (7 in.) Straight 501744 ...... US$62 501744-G* . . . US$114 Curved 501745 ...... US$62 501745-G* . . . US$114

Metzenbaum Scissors, SuperCut 11.5 cm (4.5 in.), Straight 503269 ........US$56

Curved 503270 ........US$62

12.5 cm (5 in.) Straight 14214 ............ US$51 14214-G* ... US$75

Curved 14215 ............ US$51 14215-G* ... US$75

16 cm (6.2 in.) Straight 503271 .........US$78

Curved 503272 ........US$73

20 cm (7.75 in.) Straight 503273 ........US$90

Curved 503274 ........US$95

25 cm (9.75 in.) Straight 503275 ......US$101

Curved 503276 ......US$121

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Tip.Profile:

Metzenbaum Scissors, SuperCut Tungsten CarbideThese.are.the.finest.scissors.available ..They.combine.the.precise.cutting.action.of.the.razor-sharp.



1:1

1:1

Tip.Profile:

Tip.Profile:

1:1

Mayo Scissors, Tungsten Carbide Blades

Carbide is a harder metal than stainless steel and will retain its sharpness longer

Mayo Scissors

1:1

4.cm

4 .5.cm

4.cm

4 .2.cm



18.cm.long

Straight. 501261 . . . . . . US$98 501261-G*. . . . . . . . US$198

Curved. 501262. . . . . . US$98 501262-G*. . . . . . . . US$199

Mayo Scissors, SuperCut one edge micro serrated, one edge honed to the sharpness of a knife edge

14.cm.(5 .5.in .). Straight. 14216. . . . . . . . US$48 14216-G*. . . . . . . . US$83

. Curved. 14217. . . . . . . . US$48 14217-G*. . . . . . . . US$83

16.cm.(6 .3.in .). Straight. 503277. . . . . . . . . . . US$78

. Curved. 503278. . . . . . . . . . . US$84

20.cm.(7 .8.in .). Straight. 503279. . . . . . . . . . . US$90

. Curved. 503280. . . . . . . . . . US$95

25.cm.(9 .8.in .). Straight. 503281. . . . . . . . . US$101

. Curved. 503282. . . . . . . . US$121

14.cm.(5 .5.in .)

Straight 500218 . . . . . .US$67 500218-G*. . . . . . . . US$97

Curved 500219 . . . . . . US$71 500219-G*. . . . . .US$100

17 .5.cm.(7.in .)

Straight 500220 . . . . . . US$71 500220-G*. . . . . . . . US$97

Curved 500221 . . . . . . US$71 500221-G*. . . . . .US$100

14.cm.(5 .5.in .)

Straight 501749 . . . . . . US$17 501749-G*. . . . . . . . . . . .US$37

Curved 501750 . . . . . . US$17 501750-G*. . . . . . . . . . . .US$37

17.cm.(6 .75.in .)

Straight 501751. . . . . . . US$18 501751-G*. . . . . . . . . . . . .US$39

Curved 501752 . . . . . . US$18 501752-G*. . . . . . . . . . . .US$39



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Tip.Profile:

Tip.Profile:

1:1

1:1

Tip.Profile:

1:1

Tip.Profile:

Mayo Scissors, SuperCut Tungsten CarbideThese.are.the.finest.scissors.available ..They.combine.the.precise.cutting.action.of.the.razor-sharp.



1:1

5 .5.cm

2 .5.cm

2 .5.cm

4.cm



Eye Scissors, Blunt, Probe Points, 10 cm (4 in.)

Straight. 500366. . . . . . .US$37

500366-G* . . . .US$63

Curved. 500367 . . . . . . .US$37

500367-G* . . . US$66

Iris Scissors, SuperCut Angledone edge micro serrated, one edge honed to the sharpness of a knife edge

10.cm.long

30°.angled.to.side

500046 . . . . . .US$59

Angled Scissors14.cm.long30°.angled.to.sidesharp/sharp.tips

500047. . . . . . . US$16

500047-G*. . . .US$54

Straight,.17.cm.(6 .75.in .).long. 501259. . . . . . . . . . US$98

501259-G*. . . . . . US$201

Curved,.17.cm.(6 .75.in .).long. 501260. . . . . . . . . . US$98

501260-G*. . . . . . US$202

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Tip.Profiles:

1:1

Operating Scissors

1:1

Blunt/Blunt

Sharp/Sharp

Sharp/Blunt 14192

15924

4.cm

5.cm



11.5 cm (4.5 in.) Straight

501753 Sharp/Blunt.. .. .. .. .. .. .. .. .. .. .. ..US$17 501753-G* . . . . . US$72

501754 Sharp/Sharp. . . . . . . . . . . . US$17 501754-G* . . . . . US$72

501743 Blunt/Blunt. . . . . . . . . . . . . US$17 501743-G* . . . . . US$72

11.5 cm (4.5 in.) Curved


501756 Sharp/Blunt . . . . . . . . . . . . US$17 501756-G* . . . . . US$78


14 cm (5.5 in.) Curved




14 cm (5.5 in.) Straight

14192 Sharp/Blunt.. .. .. .. .. .. .. .. .. .. .. . US$18 14192-G* . . . . . . US$33



16 cm (6.25 in.) Straight


501224 . Blunt/Blunt. . . . . . . . . . . . . US$18 501224-G* . . . . . US$33


16 cm (6.25 in.) Curved




18 cm (7 in.) Straight

15924. Sharp/Sharp . . . . . . . . . . . US$20. 15924-G* . . . . . . US$44

500378. Sharp/Blunt. . . . . . . . . . . . US$20. 500378-G*. . . . . US$44.

500379. Blunt/Blunt . . . . . . . . . . . . US$20. 500379-G*. . . . . US$44

18 cm (7 in.) Curved

501229. Sharp/Blunt. . . . . . . . . . . . US$20 501229-G* . . . . . US$44

501230. Blunt/Blunt . . . . . . . . . . . . US$20 501230-G* . . . . . US$44

501231. Sharp/Sharp . . . . . . . . . . . US$20 501231-G* . . . . . US$44



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Ceramic Zirconia ScissorsDeveloped.for.applications.where.metal.

components.were.used,.these.scissor.blades.

are.made.of.a.high.tech.ceramic.second.in.

hardness.only.to.diamond ..

The.ultra.sharp.blades.cut.with.precision.and.

accuracy.and.maintain.their.razor.sharp.edge.

for.an.extended.time ..

Perfect.for.use.in.

environments.where.rust.

and.corrosion.is.a.problem ..

Safe.for.light.duty.

electrical.use ..

1:1


1:1

1:1

Titanium

1:1

l Ceramic blades hold their sharp edge up to 100 times longer than conventional stainless steel.l Non-corrosivel No electrical static dischargel Non-magnetic l Can be used in high temperature environmentsl No metal contamination



Utility Scissor.5 .5”

501322. . . . . . . . . . . . . US$17.

501322-G*. . . . . . . . . .US$33

Variety of colored handles available!

Ceramic Scissors5 .5”.long,.2-inch.ceramic.blades,.straight

501256. . . . . . US$47

Lister Bandage scissors500444 15.cm.(6.in .).long,.4cm.long.blades . . US$25. 500444-G*. . . . . . . . . US$44

501701 8 .75.cm.(3 .5.in .).long . . . . . . . . . . . . . . US$16. 501701-G* . . . . . . . . . .US$35

501702 10 .75.cm.(4 .25.in .).long. . . . . . . . . . . . .US$17. 501702-G*. . . . . . . . . .US$37

555656S Bandage.scissors . . . . . . . . . . . . . . . . . US$138

Ligature scissors500380 9.cm.(3 .5.in .).long. . . . . . . . . US$25. 500380-G*. . . . . . . US$48

501703 10 .75.cm.(4 .25.in .).long. . . . US$26. 501703-G*. . . . . . . US$48

501704 13 .25.cm.(5 .25.in .).long. . . . US$28. 501704-G*. . . . . . . US$48

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Tip.Profile:

1:1

Tip.Profile:

1:1

1:1

Tip.Profile: 1:1

Tip.Profile: 1:1

1:1

Tip.Profile:



Scissors, Round handled14.cm.(5 .5.in .)round.Hollow.handlescurved.blades

14208. . . . . . .US$148

Microintraocular scissors14.cm.(5 .5.in .)30°.angled.blades

500372. . . . . US$299

500372-T. . . US$368

Scissors, Round handled14.cm.(5 .5.in .)round.Hollow.handlesstraight.blades

14212 . . . . . . .US$148


500374. . . . . US$299


500376. . . . . US$299

Roger Wirecutting Scissors12.cm.(4 .75.in .).longnotched.blade

501321. . . . . . . US$23

501321-G*. . . . US$44



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Tip.Profile:

7.cm

1:1

Needle Holders

Tip.Profile:

1:1Titanium

1:1Tip.Profile:

Tip.Profile: 1:1

Cuts vessels perpendicularly and without slipping.




Micro Alligator Scissors7.cm.long.shaft45°.angled,.1 .5mm.blades

500445 . . . . . . . . . . US$230

Needle Holder14.cm.(5 .5.in .).longround.handles0 .3.mm.tips,.straight.smooth.jaws

14080. . . . . . . . US$91

14080-G*. . . US$229

14.cm.(5 .5.in .).long,.straight.smooth.jaws

14361 . . . . . . US$229

14.cm.(5 .5.in .).long,.curved.smooth.jaws

501923. . . . . US$229

Biemer scissorsstraight,.13.cm.(5.in .)

500381. . . . . . . US$98

Barraquer Iris Scissors6.cm.(2 .5.in .).long7.mm.blades,.sharp.points

14128 . . . . . . . . US$91

14128-G* . . . US$229

Student Vannas ScissorsLength:. 9cm.Blades:. 8.mm.longTips:. 500.microns

Straight 501777. . . . . . . . . . . . . . . . . . . . . . . . . . . . .US$69Curved 501930. . . . . . . . . . . . . . . . . . . . . . . . . . . .US$69

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1:1

Tip.Profile:

1:1


Tip.Profile:

1:1

1:1

Tip.Profile:

1:1

Titanium

1:1

Titanium

0 .81mm

2 .30mm



Needle Holder12 .5.cm.(5.in .).longround.handles0 .4.mm.tipscurved.smooth.jaws

14132 . . . . . . . . . . US$89

Stevens Needle Holder13.cm.(5.in .).longstraight.1.mm.wide.serrated.tips

14133 . . . . . . . . . . .US$74

14133-G* . . . . . . US$240

Needle Holder14.cm.(5 .5.in .).longround.handles0 .4.mm.tips,.curved.smooth.jaws

14081 . . . . . . . .US$85

14081-G* . . . US$259

Castroviejo Needle Holder14.cm.(5 .5.in .).longserrated.Tungsten.Carbide.jawsstraight.with.lock

500223. . . . . . .US$90

500223-G*. . . US$269

Castroviejo Needle Holder14.cm.(5 .5.in .).longstraight.1 .2-mm.wide.serrated.tipswith.lock

14137 . . . . . . . . US$72

14137-G* . . . . US$224

555419NT. . . US$313

Barraquer Needle Holder

12.cm.(4 .75.in .).longcurved.with.lock .smooth.jaws

555403NT. . . . . US$299



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Tip.Profile:

Tip.Profile:

1:1

1:1

Tip.Profile:

1:1

1:1

Titanium

1:1

Titanium

1:1

Titanium0 .82mm

2 .44mm

0 .85mm

2 .37mm

0 .55mm

2 .26mm



Needle Holder14.cm.(5 .5.in .).longstraight.serrated.jaws

14110 . . . . . . . . US$16

14110-G* . . . . .US$64

Needle Holder12 .5.cm.(5.in .).longstraight.serrated.jawsextra.delicate

14109 . . . . . . . . US$16

14109-G* . . . . .US$64

Needle Holder

Needle.holder,.12.cm.(4 .75.in .).long

curved,.standard.range,.without.lock .

smooth.jaws

555400NT. . US$267

Troutman Needle Holder

12cm.(4 .75.in .).long10.mm.light.curved.jawswith.lock .smooth.jaws

555407NT. . . . . .US$313

Troutman Needle Holder.12.cm.(4 .75.in .).long10.mm.light.curved.jawswithout.locksmooth.jaws .

555406NT. . . . . US$294

Needle Holder14.cm.(5 .5.in .).longstraight.serrated.Tungsten.Carbide.jaws

15926. . . . . . . .US$55

15926-G*. . . . US$101

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1:1

Tip.Profile:

Tip.Profile:

1:1

1:1

Tip.Profile:

Can be usedambidextrously!

1:1

Tip.Profile:

Tip.Profile:

Tip.Profile:

1:1

1:1



Derf Needle Holder12.cm.(5.in .).long

501246. . . . . . . US$18

501246-G*. . . .US$45

Ryder Needle Holder13.cm.(5.in .).longserrated.Tungsten.Carbide.jaws

500226. . . . . . .US$58

500226-G*. . .US$162

Crile-Wood Needle Holder14 .5.cm.(5 .75.in .).longserrated.Tungsten.Carbide.jaws

500224. . . . . . .US$60

500224-G*. . .US$104

Mathieu Fine Needle Holder14.cm.(5 .5.in .).longserrated.Tungsten.Carbide.jaws

500225. . . . . . .US$75

500225-G*. . .US$145

Needle Holder, Round handled14.cm.(5 .5.in .).longround.Hollow.handlesstraight.smooth.jaws

14207 . . . . . . . . . US$154

Needle Holder, Round handled14.cm.(5 .5.in .).longround.Hollow.handlesurved.smooth.jaws

14206. . . . . . . . . US$154



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1:1

1:1


Tip.Profile:

Tip.Profile:

Tip.Profile:



Olsen-Hegar Needle holder with Suture Scissors,serrated.Tungsten.Carbide.jaws

14.cm.(5 .5.in .).long

500227. . . . . . . . . . . . US$66 500227-G*. . . . . . . . US$114

12.cm.(4 .75.in .).long.

501312 . . . . . . . . . . . US$102

16 .5.cm.(6 .5.in .).long

501725 . . . . . . . . . . . . US$89 501725-G* . . . . . . . . US$161

Olsen-Hegar Needle Holder14cm.(5 .5.in .).longcombined.with.Suture.Scissorsserrated.jaws

500023. . . . . . .US$37

500023-G*. . . .US$59

Micro Olsen-Hegar Needle Holder12.cm.(4 .75.in .).longcombined.with.Suture.Scissorsserrated.jawsjaw.length.10.mmtip.width.2.mm

501989. . . . . . .US$60

Castro Olsen-Hegar Needle Holder15.cm.(6.in .).longcombined.with.Suture.Scissorsserrated.jaws

501998. . . . . . US$114

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Needles 00

0

1

2

3

4

5

6

7

8

0

1

2

3

4

5

6

7

8

9

10

00

0

1

2

3

00

0

1

2

3

4

5

6

7

8

0

1

2

3

4

6

5

7

8

9

10

00

0

1

2

3

All.needles.have.a.Spring.Eye.which.allows.the.suture.to.be.pressed.into.the.eye.instead.of.threaded .

Taper.point.needles.pierce.and.spread.

tissue.without.cutting.it ..They.are.ideal.for.suturing.

delicate,.soft.tissue.when.mini-mal.trauma.is.desired .

Cutting.edge.needles.have.sharp.edges.that.penetrate.easily.through.tough.tissue ..They.are.ideal.for.suturing.skin.and.dense.dermal.tissue ..

Needle.measurements.taken.across.needle.from.eye.to.tip:

15mm



Surgical.Needles,.cutting.edge,.3/8.circle

501802. Size.00,.18mm,.pack.of.12. US$23501803. Size.0,.21mm,.pack.of.12. US$23501804. Size.1,.24mm,.pack.of.12. US$23501805. Size.2,.28mm,.pack.of.12. US$23501806. Size.3,.32mm,.pack.of.12. US$23501807. Size.4,.36mm,.pack.of.12. US$23501808. Size.5,.40mm,.pack.of.12. US$23501809. Size.6,.45mm,.pack.of.12. US$23501810. Size.7,.50mm,.pack.of.12. US$23501811. Size.8,.55mm,.pack.of.12. US$23

Surgical.Needles,.cutting.edge,.1/2.circle

501791. Size.0,.15mm,.pack.of.12. US$23501792. Size.1,.18mm,.pack.of.12. US$23501793. Size.2,.21mm,.pack.of.12. US$23501794. Size.3,.24mm,.pack.of.12. US$23501795. Size.4,.28mm,.pack.of.12. US$23501796. Size.5,.32mm,.pack.of.12. US$23501797. Size.6,.36mm,.pack.of.12. US$23501798. Size.7,.40mm,.pack.of.12. US$23501799. Size.8,.45mm,.pack.of.12. US$23501800. Size.9,.50mm,.pack.of.12. US$23501801. Size.10,.55mm,.pack.of.12. US$23

Eye.Needles,.cutting.edge,.5/16.circle(above.left)

501957. Size.00,.11mm,.pack.of.12. US$23501958. Size.0,.13mm,.pack.of.12. US$23501959. Size.1,.15mm,.pack.of.12. US$23501960. Size.2,.18mm,.pack.of.12. US$23501961. Size.3,.21mm,.pack.of.12. US$23

Eye.Needles,.taper.point,.5/16.circle(above.right)

501962. Size.00,.11mm,.pack.of.12. US$23501963. Size.0,.13mm,.pack.of.12. US$23501964. Size.1,.15mm,.pack.of.12. US$23501965. Size.2,.18mm,.pack.of.12. US$23501966. Size.3,.21mm,.pack.of.12. US$23

Surgical.Needles,.taper.point,.3/8.circle

501947. Size.00,.18mm,.pack.of.12. US$23501948. Size.0,.21mm,.pack.of.12. US$23501949. Size.1,.24mm,.pack.of.12. US$23501950. Size.2,.28mm,.pack.of.12. US$23501951. Size.3,.32mm,.pack.of.12. US$23501952. Size.4,.36mm,.pack.of.12. US$23501953. Size.5,.40mm,.pack.of.12. US$23501954. Size.6,.45mm,.pack.of.12. US$23501955. Size.7,.50mm,.pack.of.12. US$23501956. Size.8,.55mm,.pack.of.12. US$23

Surgical.Needles,.taper.point,.1/2.circle

501936. Size.0,.15mm,.pack.of.12. US$23501937. Size.1,.18mm,.pack.of.12. US$23501938. Size.2,.21mm,.pack.of.12. US$23501939. Size.3,.24mm,.pack.of.12. US$23501940. Size.4,.28mm,.pack.of.12. US$23501941. Size.5,.32mm,.pack.of.12. US$23501942. Size.6,.36mm,.pack.of.12. US$23501943. Size.7,.40mm,.pack.of.12. US$23501944. Size.8,.45mm,.pack.of.12. US$23501945. Size.9,.50mm,.pack.of.12. US$23501946. Size.10,.55mm,.pack.of.12. US$23



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How it works:



Reflex Clip Applier11.cm.(4 .3.in .).long

for.7.mm.clips.only

500343 . . . . . US$314

Reflex Clip 7 mmfor.use.with.#500343

100/box,.Stainless.Steel,.non-sterile

500344 . . . . . .US$64

Reflex Clip Applier11.cm.(4 .3.in .).long

for.9.mm.clips.only

500345 . . . . . US$314

Reflex Clip 9 mmfor.use.with.#500345

100/box,.Stainless.Steel,.non-sterile

500346 . . . . . .US$64

Reflex clip removing forceps.12.cm.(4 .75.in .).long,.for.7.mm.and.9.mm.clips500347. . . . . . . . US$153

Disposable Skin StaplerManipler.Disposable.Skin.Staplersterile,.6.staplers.per.box,.35.staples.per.stapler,.7mm

501726. . . . . . US$132

Applying Forceps.for.Silver.Clips.#500087,.18.cm.(7.in .).long

500088 . . . . . . . US$47

500088-G* . . . US$173

Silver Clips,.Micro.Vascular..0 .5.x.0 .8.mm.(100/pkg)

500087. . . . . . US$28

Staple Remover12.cm.(4 .5.in .).long,.for.use.with.disposable.skin.stapler

501830. . . . . . US$102

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Frog Heart Clips

Clips and Clamps

Vessel Clips

Bulldog Clamps

Side and front view illustrations of straight micro vessel clips



Schwartz Vessel Clips

501786-G* straight, 1.7 x 8 mm jaws US$26

501787-G* slightly angled, 1.7 x 8 mm jaws US$26

501788-G* strong angle, 1.7 x 8 mm jaws US$26

Micro Bulldog Clamp, curved 8 x 1.2 mm serrated jaws, 3 cm (1 in.) long

14119 . . . . . . US$31

14119-G* . . . US$53

Small Bulldog Clamp, straight serrated jaws, 6 cm (2.3 in.) long

14118 . . . . . . . .US$25

14118-G* . . . . US$40

Approximator, 20 g pressure, for 0.5 to 1.0 mm vessel

14084 US$36

Johns Hopkins Bulldog Clamp, 6.5 cm (2.5 in.) long, straight serrated jaws

14116 . . . . . . . US$25

14116-G* . . . . US$45

Johns Hopkins Bulldog Clamp, curved serrated jaws, 6.5 cm (2.5 in.)long

14117 . . . . . . US$25

14117-G* . . . US$45

Applying Forceps for Approximator #14084 & Vessel Clips #14120, #15911, and #14121, 10 cm (4 in.) long

14189 US$32

15911 10 g pressure, 5 x 0.8 mm jaws, 5 per pack US$61



501902 ...US$56two flat loops2.5 mm diameter loops24 mm long5 per package

501903 ... US$37two fine loops0.6 mm diameter tips17 mm long5 per package

501904 ...US$56

two flat tips1 x 3 mm tips18 mm long5 per package

Micro Vessel Clips, serrated jaws

501779-G* straight, 0.75 x 4 mm jaws US$68501780-G* straight, 0.8 x 6 mm jaws US$68501781-G* straight, 1 x 8 mm jaws US$68501782-G* straight, 1.5 x 10 mm jaws US$68

501783-G* slightly curved, 1 x 6 mm jaws US$82501784-G* fully curved, 1 x 6 mm jaws US$82501785-G* 45 deg angle, 1 x 6 mm jaws US$82



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Retractors

Picture is smaller than actual size

1:1

1:1

small. medium. large

1:1

Titanium

1:1

1:1



KD Mouse/Rat Eye Speculum. .

501897 Small,.2 .1cm.long,.2 .2mm.blades,.4 .3mm.max ..spread. US$26501898 Medium,.2 .1cm.long,.3 .2mm.blades,.4 .3mm.max ..spread.US$26501899 Large,.2 .1cm.long,.4mm.blades,.3 .2mm.max ..spread. US$26

Wire SpeculumLength:. . 4 .5.cm.(1 .75.in .).Blades:. . 13 .5.mm.blades.Maximum.spread:. 20.mm.

555801L. . . . . .US$32

Barraquer Retractor4.cm.(1 .5.in .).long,

10.x.4.mm.wire.blades

7.mm.depth

maximum.spread.3.cm

500369 . . . . . .US$26500369-G* . . .US$35

Wire Retractors5.cm.(2.in .).long

10.x.5.mm.blades

5.mm.depth

maximum.spread.13.mm

14130 . . . . . . . . US$1614130-G* . . . . .US$24

Wire Retractors5.cm.(2.in .).long,


7.mm.depth


500368 . . . . . .US$26500368-G* . . . US$51

Wire Retractors.

4.cm.(1 .6.in .).long

13..x.5.mm.wire.blades

7.mm.depth


501994. . . . . . .US$28

Wire Retractors4.cm.(1 .6.in .).long


5.mm.depth


501993. . . . . . .US$28

Wire Retractors - non-magnetic4 .5.cm.(1 .8in .).long

0 .8.x.1 .5.mm.wire.blades


503210. . . . . . .US$28

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Maximum.spread.55.mmMaximum.spread.15.mm1:1



Agricola Retractor.

Length:. 4.cm.(1 .6.in .)Blades:. 3.x.3.sharp.prongs.Small,.self-retaining.retractor.perfect.for.small.animal.surgery.and.dissection .

501846. . . . . . . . . . . . . . . US$91.

Goldstein Retractor.Small,.self-retaining.retractor.perfect.for.small.animal.surgery.and.dissection .

Length:. 2.cm.(0 .78.in .)Blades:. 3.x.3.sharp.prongs.Maximum.Spread:. 1 .0cm

501967. . . . . . . . . . . . . . US$132


501968. . . . . . . . . . . . . . US$114


501969. . . . . . . . . . . . . . US$114.

Stevenson Retractor,.8.cm.(3.in .).long,.3x3.prongs,.3.mm.deep

14131 . . . . . . . .US$6614131-G* . . . . .US$97

ALM Self-retaining Retractor,.7.cm.(2 .75.in .).long,.4x4.prongs

14240. . . . . . . . US$3714240-G*. . . . . US$87

Modified ALM Self-retaining Retractor7.cm.(2 .75.in .).long,.2x2.prongs

Smaller,.thinner.prongs.for.use.on.mouse/rat.eyelids

501844. . . . . . . US$37

Knapp Speculum,.15.x.6.mm.blades,.8 .3.cm.(3 .25.in .).long,.maximum.spread.25.mm

14129. . . . . . . . US$5114129-G*. . . . US$277

Self-retaining Retractor.

Length:. 5.cm.(2.in .)Blades:. 2 .5.cmMaximum.spread:....4 .5.cm.Small,.self-retaining.retractor.perfect.for.small.animal.surgery.and.dissection .

503209 . . . . . . . . . . . . . . US$91.



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Jansen retractor4 .5.in ..long,.maximum.spread.3cm

3.x.3.prongs,.blunt

501770 . . . . . . .US$64501770-G* . . US$288

sharp.prongs

501859. . . . . . .US$64

Weitlaner Retractor, self-retaining

501328. . . . . . . . . . . . .US$109 501328-G* . . . US$3574”,.2x3.prongs,.blunt,.maximum.spread.3cm

501314 . . . . . . . . . . . . .US$109 501314-G* . . . US$3574”,.2x3.prongs,.sharp,.maximum.spread.4cm

501722 . . . . . . . . . . . . .US$121 501722-G* . . . US$2885 .5”,.3x4.prongs,.blunt,.maximum.spread.4 .5cm.

501721 . . . . . . . . . . . . .US$121 501721-G* . . . .US$3615 .5”,.3x4.prongs,.sharp,.maximum.spread.4cm

501724 . . . . . . . . . . . . .US$138 501724-G* . . . US$3996 .5”,.3x4.prongs,.blunt,.maximum.spread.6 .5cm

501723 . . . . . . . . . . . . .US$138 501723-G* . . . US$3996 .5”,.3x4.prongs,.sharp,.maximum.spread.7cm

Gelpi Retractor

3 .5” 501329 . . . . . . .US$86

4 .5” 501719. . . . . . . .US$98

5 .5” 501720 . . . . . .US$109 501720-G* . . . . . US$342

Senn Retractor7”

sharp 501327 . . . . . . US$29 501327-G* . . . . . .US$84 blunt 501718 . . . . . . US$29 501718-G*. . . . . . .US$84

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Custom sizes available— call for details

Sapphire Knife Protection Tray

Sapphire Knife

Clear blade increases view of tissue

Chemically inert to lymph, blood, and disinfectant solutions Single.crystal.sapphire.(Al2O3),.while.not.as.hard.as.diamond,.is.still.hundreds.of.times.

harder.than.steel.razor.blades ..The.durability.of.the.blade.guarantees.a.unique.operational.life.that.exceeds.one.hundred.incisions.In.addition,.the.blade.and.handle.can.be.sterilized.using.any.currently.known.procedure ..

The.super.sharp.cutting.edge,.mirror.surface.finish,.and.low.friction.coefficient.combine.to.create.an.accurate,.clean.cut.with.minimal.tissue.trauma ..Scarring.is.thus.reduced.and.incisions.heal.quickly ..

Both.the.stainless.steel.and.titanium.handles.feature.safety.locks. to. protect. the. super. sharp. cutting. edge. from. damage. and.prevent.injuries.to.medical.personnel ...

60°90°30°

Crescent.Bladefront & side view

Phaco.Bladefront & side view

500313 500316 501736

501761 501762

501

76

3.(b

lade

.sol

d.se

para

tely

)

50

031

7.(b

lade

.sol

d.se

para

tely

)



Protect.your.sapphire.knife.with.a.sterilization/protection.tray ..Fully.autoclavable.tray.safely.holds.two.sapphire.knife.handles.for.sterilization.and.storage .

501729. Sterilization/Protection.Tray. US$35

500317 Stainless.Handle.for.Sapphire.Knife,.13.cm.long,.retractable.. US$56501811 Titanium.Handle.for.Sapphire.Knife,.13.cm.long,.retractable. US$138

Single Edge Blades for use with above handles 500317 & 501811501760 Sapphire.Blade,.single.edge,.0 .22mm.thick,.1.mm.wide,.15°.. US$45500313 Sapphire.Blade,.single.edge,.0 .2mm.thick,.1.mm.wide,.30°.. US$45501304 Sapphire.Blade,.single.edge,.0 .23mm.thick,.1.mm.wide,.45°.. US$45501735. Sapphire.Blade,.single.edge,.0 .21mm.thick,.1.mm.wide,.60°. US$45501736. Sapphire.Blade,.single.edge,.0 .3mm.thick,.1.mm.wide,.90°. US$45500315 Sapphire.Blade,.single.edge,.0 .21mm.thick,.2.mm.wide,.30°.. US$75500325 Sapphire.Blade,.single.edge,.0 .3mm.thick,.1 .4.mm.wide,.35°.. US$75

Double Edge Blades for use with above handles 500317 & 501811500314 Sapphire.Blade,.double.edge.lance,.0 .3mm.thick,.1.mm.wide,.35°. US$63500316 Sapphire.Blade,.double.edge.lance,.0 .3mm.thick,.2.mm.wide,.60°.. US$121500326 Sapphire.Blade,.double.edge.lance,.0 .3mm.thick,.1 .7.mm.wide,.60°. US$137

Specialty Handle and Blades501763. Stainless.Angled.Handle.for.Sapphire.Knife,.13.cm.long,.retractable. US$56501812 Titanium.Angled.Handle.for.Sapphire.Knife,.13.cm.long,.retractable. US$138501761. Crescent.Sapphire.Blade,.2 .8.mm.wide,.0 .3mm,..(fits.handles.501763.&.501812). US$190501762. Phaco.Sapphire.Blade,.2 .7/3 .4.mm.wide,.0 .3mm,..(fits.handles.501763.&.501812). US$201



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Sterile stainless steel blades

10

11

12

15

20

21

22

23

24

For Blades #10 ~ #15

All.stainless.steel.scalpel.blades.are.made.by.Feather®,.using.a.precise.beveling.technique.to.create.the.edge’s.micron.sharpness ..They are the finest blades available.



Scalpels, Knives, Blades and Handles

1:1



Scalpel Handle #313.cm.(5.in .).long,.stainless.steel

500236 . . . . . . . . . . US$9

500236-G*. . . . . . .US$25

Scalpel Handle #414.cm.(5 .5.in .).long,.stainless.steel

500237. . . . . . . . . . . US$9

500237-G*. . . . . . .US$25

Scalpel Handle #7

16.cm.(6 .25.in .).long,.stainless.steel500238 . . . . . . . . .US$13500238-G*. . . . . . .US$41

500239. Scalpel.Blades.#10,.stainless.steel,.sterile.(100./.box).. US$41500240. Scalpel.Blades.#11,.stainless.steel,.sterile.(100./.box).. US$41500241. Scalpel.Blades.#12,.stainless.steel,.sterile.(100./.box).. US$41500242. Scalpel.Blades.#15,.stainless.steel,.sterile.(100./.box).. US$41500243. Scalpel.Blades.#20,.stainless.steel,.sterile.(100./.box)..US$44500244. Scalpel.Blades.#21,.stainless.steel,.sterile.(100./.box).. US$44500245. Scalpel.Blades.#22,.stainless.steel,.sterile.(100./.box)..US$44500246. Scalpel.Blades.#23,.stainless.steel,.sterile.(100./.box)..US$44500247. Scalpel.Blades.#24,.stainless.steel,.sterile.(100./.box)..US$44500248. Scalpel.Blades.#25,.stainless.steel,.sterile.(100./.box)..US$44

Round Knurled Blade Handle,.stainless.steel,.10.cm.long

501247. . . . . . . . . US$36501247-G* . . . . . . US$58

Hex Blade Handle, stainless steel,.10.cm.long

501248. . . . . . . . . . US$36501248-G*. . . . . . . US$58

501249 Needle.Blades,.6/pk,.4 .5.cm. . . . . . . . . . . . . . . . . . . .US$104

501250 Miniature.Edged.Blades,.curved.tip,.12/pk,.3 .5.cm . . US$18

501251 Miniature.Edged.Blades,.round.tip,.12/pk,.3 .5.cm. . .US$22

Blade Safe surgical blade removerwill.remove.blades.easily.and.safely.from.any.style.surgical.blade.handle

501335. . . . . . US$25

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Microsurgical KnivesSterile,.disposable

1:1

1:1

Special jaw shape prevents blades from slipping

Tip.Profile:

Tip.Profile:

1:1



500348 Disposable.Scalpel,.No ..10,.sterile,.(10/box). US$12

500349 Disposable.Scalpel,.No ..11,.sterile,.(10/box) US$12


500351 Disposable.Scalpel,.No ..15,.sterile,..(10/box) US$12






500249. Microsurgical.Knife,.15°.5.mm.blade,.13 .5.cm.(5 .25.in .).long.(package.of.6)......US$79

500250. Roundstock.Ophthalmic.Knife,.15°.5 .7.mm.blade,.13 .5.cm.(5 .25.in .).long.(package.of.6). US$97

501731. Microsurgical.Knife,.15°.3.mm.blade,.13 .5.cm.(5 .25.in .).long.(package.of.6). US$79

Disposable Safety Scalpels

Retractable.blade.protects.from.accidental.injury ..Complies.with.the.OSHA.Directive ..

501337. Size.#10,.pack.of.10. . . . . US$41501338. Size.#11,.pack.of.10. . . . . US$41501339. Size.#15,.pack.of.10. . . . . US$41

Razor Blade Holder12 .5.cm.(5.in .)..longround.tips.S-shape.jaws

14139 . . . . . . . . . .US$112

Razor Blade Holder12.cm.(4 .75.in .).longround.tipsconvex/concave.jaws

14134 . . . . . . . .US$89

14134-G* . . . US$290

Micro Dissecting Knife.

13.cm.long,.1.mm.blade,.right.angle

14135 . . . . . US$52



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Rongeurs, Cutters, and Punches

1:1

Tip.Profile:

1:1

1:1

Ear Punches and Ear Tags



Rongeur, Micro Friedman16.cm.(6 .25.in .).long.Jaw.Width

1 .3mm 14292. . . . . . .US$81 14292-G* . . . US$2020 .8.mm. 501332. .. .. .. .. US$851 .0.mm. 501333. .. .. .. .. US$83

Ear Punch 10.cm.(4.in .).long

2.mm.diameter. 500077. .. .. .. ..US$521.mm.diameter. 500076. .. .. .. ..US$52

Ear Punch5.cm.(2.in .).long,.2.mm.diameter

500075. . . . . . .US$22

Ear Tag Applicator

501876 . . . . . . .US$48

501866 Ear.Tags,.pack.of.100,.sequence.000-099. US$35

501867 Ear.Tags,.pack.of.100,.sequence.100-199 US$35










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1:1

1:1

1:1

Tip.Profile:

Tip.Profile:

Tip.Profile:

1:1

The Corneoscleral punch is excellent for removing the skull of a small animal without damaging the brain or spinal cord. The lower jaw of the punch is a flat plate which can be inserted into the skull with much less damage to the brain than a ronguer.

Tip.Profile:

1:1

Cutting.Blade

Cutting.Block



Friedman.Rongeur13.cm.(5.in .).long

jaw.width.2 .5.mm

14089. . . . . . . .US$6914089-G*. . . .US$183

Mini Blumenthal.Rongeur11.cm.(4 .3.in .).long

jaw.width.2.mm

14090. . . . . . . . US$8114090-G*. . . . US$192

500079 Corneoscleral.Punch.,.8.cm.(3.in .).long,.2.mm.wide. US$74500143 Corneoscleral.Punch,.8.cm.(3.in .).long,.1 .5.mm.wide. US$74

Blumenthal.Rongeur15.cm.(6.in .).long

jaw.width.3.mm

14091 . . . . . . . .US$6814091-G* . . . .US$189

Single Blade Bone Cutter14.cm.(5 .5.in .).long

jaw.length.23.mm

blade.tip.width.1.mm

block.tip.width.2.mm

501990. . . . . . .US$83



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1:1

1:1

Tip.Profile:

1:1

Tip.Profile:

Tip.Profile:

Tip.Profile:

Tip.Profile:

Applications

● MRI

● Marine.biology

Never Rusts!



Cuticle nipper11.cm.(4 .3.in .).long,.angle,.ring.handles

500137. . . . . . .US$25500137-G* . . . .US$94

Liston Bone Cutting Forceps19.cm.(7 .5.in .).long,.straight.. 500358. . . . . . US$6014.cm.long,.straight.. 500462. . . . . . US$52

Titanium Wire Cutter15.cm.(6.in .).long,.15.mm.Tungsten.Carbide.blades,..light.weight.(60g)

500450 . . . . . . . . US$276

Nail nippers14.cm.(5 .5.in .).long,.concave.jaws,.double.spring

500362 . . . . . . US$18500362-G*. . . .US$97

Malleus Nipperone.serrated.blade,.11.cm.(4 .3.in .).long,.9.mm.blades

501269. . . . . . .US$90

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Hooks and Probes

500135

500133

500134

Tungsten Dissection Probes●Used.for.separating.cells.

●Made.of.pure.tungsten,.a.hard.and.biocompatible.metal

●Less.than.one.micron.in.diameter

●Much.stronger.and.more.durable.than.glass.probes

●All.probes.are.50.mm.long.(10.pieces.per.package)

1:1

Tip Profile BTip Profile A

Illustration.-.not.shown.actual.size



Tip Profile A500133 Tungsten.Dissecting.Probe,.0 .125mm.diameter.rod.. US$83500134 Tungsten.Dissecting.Probe,.0 .25mm.diameter.rod.. US$83500135 Tungsten.Dissecting.Probe,..0 .5mm.diameter.rod.. US$83

Tip Profile B501316 Tungsten.Dissecting.Probe,.0 .125mm.diameter.rod.. US$83501317 Tungsten.Dissecting.Probe,..0 .25mm.diameter.rod.. US$83501318 Tungsten.Dissecting.Probe,.0 .5mm.diameter.rod.. US$83

Handle for Probe,.stainless.steel.

11.cm.(4 .3.in .).long 500448 . . . . . . US$48

7.cm.(2 .75.in .).long 501301 . . . . . . US$48

Titanium Flexible Fine Probe.50.mm.long,.less.than.5.micron.tip,..0 .4mm.shaft,.pkg.of.2

500449 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .US$83

.Probe,.stainless.steel,.14cm.(5 .5.in .),.1 .0.mm.diameter. .501313 . . . . . .US$9



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1:1



500458 Straight Dissecting Needles,.14.cm.(5 .5.in .).long,.12/box. US$14

500460 Curved Dissecting Needles,.14.cm.(5 .5.in .).long,.12/box. US$14

Meyhoefer Curette14cm.(5 .5.in .),.1 .5mm.diameter

501773 . . . . . . .US$33

501773-G* . . . US$151

Wecker Spatula14cm.(5 .5.in .),.3mm.wide.blade

501772 . . . . . . .US$33501772-G* . . .US$154(German-made.spatula.is.2mm.wide)

Tyrell Hook,.sharp,.13.cm.(5.in .).long

14136 . . . . . . . .US$3014136-G* . . . . . US$51

Castroviejo Caliper75.mm.(3.in .).long

20.mm.scale

501200. . . . . .US$138

Vascular Measuring Ruler503211. . . . . .US$166

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SuperCut Tenotomy Scissors curved (#14396)Rongeur 3mm jaw (#14091)Utility Scissors (#501322)Operating Scissors straight Sharp/Blunt (#14192)Dumont Tweezer #5 (#14098)Flat Jaw Tweezers (#501303)Probe 1.0 mm diameter, blunt (#501313)Order Number: KIT-PHYSIO-I

Vannas Scissors (#14003)SuperCut Iris Scissors straight (#14218)

Rongeur 1 .3mm jaw (#14292)Utility Scissors (#501322)

Operating Scissors straight Sharp/Blunt (#14192)Dumont Tweezer #5 (#14098))

Probe 1.0 mm diameter, blunt (#501313)Stevenson Retractor (#14131)

Iris Forceps curved (#15915)Adson Forceps 1x2.teeth.(#500092)

Olsen-Hegar Needle holder (#500227) Order Number:PHYSIO-II

Physiology Kit I

Physiology Kit II

Add the Stainless Steel Protection Case to any kit (#14213)for an additional $50



US$144US$239 Value

US$293US$506 Value



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Dumont #5.(#14098)Vannas Scissors.(#14003)Iris Forceps,.curved,.serrated.(#15915)Dissecting Scissors,.straight.10cm.(#14393)Wire Retractor.(#14130)Needle Holder.(#14109)Blunt Probe, 1.0 mm diameter.(#501313)Order Number: MOUSEKIT.

Rat Kit

Dumont #5.(#14098)Vannas Scissors.(#14124)Iris Forceps,.serrated.(#15915)SuperCut Iris Scissors,.straight.10cm.(#14218)Alm Retractor.(#14240)Needle Holder.(#14110)Blunt Probe, 1.0 mm diameter.(#501313)Order Number: RATKIT.

Mouse Kit



US$163US$239 Value

US$194US$293 Value

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Mouse Vessel Cannulation KitKit includes:Vessel Cannulation ForcepsVannas ScissorsFine Serrated ForcepsDumont #5 TweezersAlm RetractorMicroFil 28 ga.

Order Number 501895

Vessel Cannulation Forceps0.5mm diameter

This.micro.cannula.is.ideal.for.placement.in.the.carotid.or.femoral.artery.of.mice,.rats,.and.other.small.animal.blood.vessels ..It.can.be.used.with.a.pressure.transducer.(WPI’S.BLPR).for.blood.pressure.measurement,.or.in.conjunc-tion.with.a.micro-syringe.injection.system.(like.WPI’s.UMPII.or.MMP.pumps) ..The.incorporated.standard.female.luer.fitting.makes.connecting.to.existing.experimen-tal.plumbing.quick.and.easy ..The.cannula.is.provided.with.a.contoured-tip.stainless.steel.stylet.(trocar).to.facilitate.placement.using.established.techniques ..A.movable.“shoulder”.ring.provides.a.tie-in.point.to.prevent.accidental.removal ..The.cannula.may.be.left.in.place.for.two.hours.or.more,.and.with.proper.care.and.cleaning,.may.be.re-used.multiple.times ..Instructions.for.use.included .

Micro Cannula

l.0.4mm O.D., 0.2mm I.D. tubingl.Autoclavablel.Biocompatible Perfluorocarbon tubing material



KZ1101. Micro.Cannula,.3". US$92

US$263US$360 value



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TurtleSkin® FullCoverageEntire Hand Protection —Fingertip to Forearm

While no glove is puncture or cut proof, Turtle-Skin FullCoverage gloves give you puncture and cut protection covering the entire hand. Surpris-ingly soft and comfortable, these gloves offer over 35 ounces of needle protection.

TurtleSkin is a registered trademark of Warwick Mills, Inc.

The small animal guillotine has been completely redesigned for ease of use and extra added safety features. The blades are drawn together by magnetic force to ensure a clean and precise cut through very strong bones and skin.

There is a large base for stability, long handle for extra leverage, spring action so the blades can not fall down unexpectedly, hardened stainless blades for endurance, simplified construction for easy maintenance. The fluoropolymer coated surface

on the base makes cleaning easy.

The guillotine is considered one of the most humane methods to dispense with a subject.

● Large, stable base● Hardened blades for

long service● Ambidextrous

configuration

Rodent Guillotine



501888 TurtleSkin® Gloves, small, pair US$91501889 TurtleSkin® Gloves, medium, pair US$91501890 TurtleSkin® Gloves, large, pair US$91501891 TurtleSkin® Gloves, extra large, pair US$91

DCAP Guillotine for Rodents and other small animals (opening 1.5 x 1.5 in.) US$569DCAP-M Guillotine for large rodents and other medium animals (opening 2.5 x 2.5 in.) US$949DCAP-L Guillotine for larger animals (opening 4” x 4”) US$1,374

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1:1



Disposable Biopsy Punches,.package.of.50Individually.packaged.in.color-coded,.transparent,.sturdy.blister.pack.to.protect.cutting.edgeSeamless.stainless.steel.razor.sharp.cutting.edgeRibbed.handle.provides.maximum.control.and.comfort

501816. 1 .5mm.diameter. US$148501817. 2 .0mm.diameter. US$148501818. 3 .0mm.diameter. US$148

Uni-Core PunchesCuts, retrieves, and stores, cored samples in one operation

The.Harris.Uni-Core.punch.consists.of.a.razor.sharp.stainless.steel.cutting.tip.designed.to.cut,.retrieve.and.store.cored.samples.from.source.materials.such.as.paper,.gels,.cloth,.leaves,.paint.chips,.films,.tissue.or.other.thin.or.soft.substrates ..The.tip.is.protected.by.a.removable.cover.cap ..The.body.is.made.from.polypropylene.plastic ..Each.Harris.Uni-Core.is.individually.pouched.and.ethylene.oxide.sterilized ..The.Uni-Core.is.a.limited.reusable,.disposable.sampling.tool ..It.may.be.disposed.of.after.use.or.autoclaved.at.20.minutes.at.250°F.and.15.p .s .i ..for.reuse .

501905 0 .50mm.ID,.1 .0mm.OD US$17501906 0 .75mm.ID,.1 .25mm.OD US$17501907 1 .25mm.ID,.1 .75mm.OD US$17501908 2 .0mm.ID,.2 .5mm.OD US$17501909 5 .0mm.ID,.5 .5mm.OD US$17501910 6 .0mm.ID,.6 .5mm.OD US$17501911 7 .0mm.ID,.7 .5mm.OD US$17501912 8 .0mm.ID,.8 .5mm.OD US$17

Achieve.hemostasis.of.bone.by.applying.a.small.amount.of.sterile.bone.wax ..The.wax.is.a.combination.of.beeswax,.isopropyl.palmitate,.and.mineral.oil.which.stops.bleeding.at.the.site ..

501771. Bone Wax,.sterile,.3g. US$29.

Applicator Spears

Made.of.PVA.these.spears.will.absorb.fluids.and.liquids .

501789. Applicator.Spears,.pkg.of.200. US$68.



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Gain control — see clearly and accurately

Unlimited uses, from surgical procedures to microcircuitry assembly

Priced so that everyone in the lab can have one!

● High.quality.coated.optics

● Adjustable.independent.focus.for.each.eye

● Adjustable.interpupillary.distance

● Mounted.on.spectacle.frames

● Protective.velvet-lined.wood.case.included

Fantastic Magnifying Glasses!

Fantastic Price!

500370.—.2 .5x.Binocular.Loupe

501331.—.4x.Binocular.Loupe

Veterinary surgeons find WPI’s binocular loupes useful for surgery on Koi fish at the International Conference on Exotics wet lab in Key West, Florida. Photo by Marc Kramer, DVM

BINOCULAR LOUPE SPECIFICATIONS

. 2.5x (500370) 4x (501331)

WORKING.DISTANCE. 35-50.cm. 38-48.cm.

FIELD.OF.VIEW. 10.cm.diam .. 6 .5.cm.diam ..



500370. Wide.Field.Binocular.Loupe,.2 .5x,.35~50.cm.working.distance,.. . .. adjustable.pupillary.distance.and.focus,.lightweight.(72.g). US$403

501331 4x.Binocular.Loupe.. US$684

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Surgery Table

SURGERY TABLE SPECIFICATIONSLength. 20.cm. . .

Width. 14 .5.cm

Height. 4.cm.(rear)

. 2.cm.(front)



Constructed.of.clear.acrylic,.this.economical.surgery.table.features.adjust-able.nylon.cords.for.fast.animal.restraint.and.a.slight.incline.for.easy.view-ing ..The.acrylic.construction.is.durable,.yet.easy.to.clean ..The.nylon.cords.can.be.adjusted.at.the.point.of.contact.with.the.animal.and.also.under.the.table.itself ..

The.surgery.table.is.designed.for.use.in.dissecting.small.animals.such.as.frogs,.birds,.mice,.rats,.guinea.pigs,.etc .

501892 Surgery.Table. US$55

Student Dissecting KitIncludes:Dissecting.Scissors,.4 .5”Dissecting.Scissors,.4”Dressing.Forceps,.5 .5”Micro.Dressing.Forceps,.4”#4.Knife.HandleStraight.Teasing.NeedleAngled.Teasing.Needle#22.Surgical.BladeCanvas.Roll-up.

501336. . . . . . . . . . . . . . . US$22

Silicone Dissecting Pad KitMake your own silicone dissecting pads easily and quickly. Mix the 2-part silicone right in the plastic petri dishes and allow to cure 24 hours at room temperature. Kit includes enough silicone to prepare 20 dishes.

Kit Includes:

2-Part.SylGard.silicone.elastomer20.plastic.petri.dishes.with.lids,.65mmPins

501986. . . . . . . . . . . . . . US$132



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Cautery

500394 tip 500393 tip

500389 handle

500394 tip

500396 tip

500396 tip

500395 tip

500390 handle

Tip.Profile:

Tip.Profile:



Disposable Adjustable High Temperature CauteryFine.Tip.(#500394)1500-2100°.F10/box,.sterile

500388. . . . . . . . . . . . . . . .US$164

High Temperature Cautery2.replaceable.batteries.(AA)1.interchangeable.tip.(#500394)2200°.F

500389. . . . . . . . . . . . . . . . US$61

High Temperature Cautery Kitcontains.1.#500389.handle,.2.#500394.tips.2.#500393.tips

500392. . . . . . . . . . . . . . . . US$71

Disposable Adjustable Low Temperature Cautery.Fine.Tip.(#500396)700-1200°.F10/box,.sterile

501090. . . . . . . . . . . . . . . US$148

Low Temperature Cautery1.replaceable.battery.(AA)1.tip.(#500396)

500390. . . . . . . . . . . . . . . . US$52

Low Temperature Cautery Kitcontains.1.#500390.handle2.#500396.tips.2.#500395.tips

500391. . . . . . . . . . . . . . . . US$71

Disposable.High.Temp.Loop.Tips,.2000°.F,.10/box,.sterile,.for.use.in.conjunction.with.#500389.and.#500392

500393. . . . . . .US$64

Disposable.High.Temp.Fine.Tips,.2200°.F,.10/box,.sterile,.for.use.in.conjunction.with.#500389.and.#500392

500394. .. .. .. .. .. .US$64

Disposable.Low.Temp.Elongated.Tips,.1100°.F,.10/box,.sterile,.for.use.in.conjunction.with.#500390.and.#500391

500395. . . . . . .US$64

Disposable.Low.Temp.Fine.Tips,.1300°.F,.10/box,.sterile,.,.for.use.in.conjunction.with.#500390.and.#500391

500396. .. .. .. .. .. .US$64

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Tradional.thermal.cautery.utilizes.heat.in.the.form.of.direct.current.to.perform.coagulation ..This.method.eliminates.the.possibility.of.alternate.site.burns.that.can.occur.with.electrosurgery ..Only.the.heated.wire.tip.comes.in.contact.with.the.tissue.and.current.does.not.enter.the.patient’s.body ..WPI’s.Thermal.Cautery.Unit.provides.all.the.cautery.power.necessary.for.treating.numerous.minor.surgical.conditions.and.is.ideal.for.applications.such.as.dermatological,.ophthalmological,.occupational.health,.ENT,.and.plastic.surgery.cases,.or.any.procedure.that.requires.accurate.hemostasis .

The.Thermal.Cautery.Unit.maintains.the.desired.temperature.during.tissue.contact.and.does.not.rapidly.diminish.like.disposable.battery.powered.cauteries ..The.autoclavable.handpiece.with.six-foot.cord.accepts.an.assortment.of.reusable.thermal.cautery.electrodes,.allowing.the.surgeon.to.have.the.flexibility.to.perform.a.wide.variety.of.procedures ..This.unit.will.outlast.battery-powered.units.and.save.money.on.replacement.batteries ..

Thermal Cautery UnitProvides consistent, accurate hemostasis

CAUTERY UNIT SPECIFICATIONS

LINE.VOLTAGE. 110V/220V,.60Hz/50Hz

OUTPUT.WAVE.SHAPE. Clipped.Sinusoidal

OUTPUT.POWER. 0-30.Watts.±.6.Watts.(0 .2.Ohm.Load)

MAXIMUM.OUTPUT.POWER. 36.Watts.(0 .2.Ohm.Load)

DIMENSIONS. 13cm.x.13cm.x.20cm.(dxwxh)

SHIPPING.WEIGHT. 5.lb.(2 .3kg)

a cb d e

Handpiece and electrodes A, C, and E are included with unit.



501292. Thermal.Cautery.Unit,.110V. US$684501293. Thermal.Cautery.Unit,.220V. US$746501294. Cautery.Handpiece.for.501292.&.501293. US$208501295. Loop.Tip.(shown.a). US$68501296. Fine.Tip-Flat.(shown.b). US$68501297 Loop.Tip-Angled.(shown.c) US$68501298. Ball.Point.(shown.d). US$75501299. Fine.Tip.(shown.e). US$68501300. Wall.Bracket.for.501292.&.501293. US$64



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This high performance electrosurgical generator

features Cut, Coagulation, Fulguration, and Bipolar

modes. The automatic safety features such as

self test circuits, audible tones, discreet outputs,

and isolated circuitry make it ideal for use in

the modern physician’s office, surgi-center, and

research lab. This generator is suitable for all types

of medical procedures and surgeries with safety,

flexibility, reliability, and convenience.

● Digital error detection

● Large illuminated digital displays

● Rotary power-control dial

provides smooth, rapid power

adjustments

Electrosurgery Systems

High Frequency Dessicator

This High Frequency Dessicator produces radio frequency current which

is useful for the removal and destruction of superficial cutaneous and

mucosal lesions. It performs dessication and fulguration procedures.

The digital power control system allows you to change from low to

high power by rotating the power knob or pushing the buttons on the

handpiece.

Check out our website for detailed ordering

information and pricing on all accessory parts and a large range of electrodes.



500421. Electrosurgical.Unit.(110V). US$2,887501213. Electrosurgical.Unit.(220V). US$2,887

500397. Dessicator.Unit.(110V)... US$1,323501214. Dessicator.Unit.(220V).. US$1,323

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Electrosurgery.utilizes.alternating.current.at.radio.frequencies.to.cut.and.coagulate ..Using.this.method,.the.current.enters.the.patient’s.body.and.the.patient.becomes.part.of.the.electrical.circuit ..This.requires.the.use.of.a.return,.or.indifference.plate ..

This.economically.priced.electrosurgical.unit.has.10.levels.of.output.intensity,.three.operational.modes.(cut,.coagulate,.and.cut/coagulate),.and.various.choices.of.electrodes ..

The.unit.comes.complete.and.ready-to-use.with.a.handpiece,.indifference.plate,.footswitch,.and.one.of.each.electrodes ..All.accessories.can.also.be.ordered.separately .

Economy Electrosurgical Unit

ELECTROSURGICAL UNIT SPECIFICATIONS OPERATION.FREQUENCY. 1 .5.MHz

STABLE.&.FINE.POWER.SETTING. 10.Steps

POWER.SUPPLY. 115V.±.10%.-.50/60Hz.1 .8A,.210VA

. . 230V.±.10%.-.50/60Hz.0 .9A,.210VA

OUTPUT.POWER. 70.WATTS.±.5%

WORKING.FRQUENCY. 1 .5-1 .7.MHZ.±.5%

DIMENSIONS. 24cm.x.22cm.x.8 .5cm.(lxwxh)

SHIPPING.WEIGHT. 10.lb.(4 .5kg). .

MICRODRILL SPECIFICATIONS

INPUT. 110V,.50/60.Hz

OUTPUT. 0-32.Vdc

FUSE. 1.amp

OPERATING.SPEED.RANGE. 0-35,000RPM

DIMENSIONS. 178.x.114.x.89mm.(7.x.4 .5.x.3 .5in .)

WEIGHT. 1 .7kg.(3 .75lbs)

Micro Drill SystemThis.line.powered.micro.drill.will.make.easy.work.of.grinding,.finish-ing,.cutting,.and.drilling.bone,.teeth,.and.other.material ..The.high-torque.35,000.rpm.(maximum).motor.is.quiet.and.has.minimal.vibration.which.reduces.wear.on.the.motor.and.provides.greater.comfort.for.the.user ..It.also.features.a.forward.and.reverse.switch,.“E.Type”.handpiece,.and.handpiece.holder ..The.handpiece.has.a.removable.nose.cone.that.can.be.cleaned.and.sterilized ..It.accepts.3/32”.and.2 .33.mm.bur.shanks .. Unlike.battery-powered.drills,.this.unit.will.maintain.consistent.power.for.the.duration.of.use ..The.wide.range.of.speeds.allows.the.user.to.control.the.amount.of.heat.generation .

The.following.accessories.are.included.with.the.Micro.Drill.System:..

Qty. Description4. Abrading.Tip,.Rubber.1. Abrading.Tip,.Stone.1. Accessory.Stand.1. Ball.Mill,.Carbide,.#1,. .031”.Diameter.1. Ball.Mill,.Carbide,.#2,. .039”.Diameter.1. Ball.Mill,.Carbide,.#3,. .047”.Diameter.1. Ball.Mill,.Carbide,.#4,. .055”.Diameter.1. Ball.Mill,.Carbide,.#5,. .063”.Diameter.1. Ball.Mill,.Carbide,.#6,. .071”.Diameter.1. Ball.Mill,.Carbide,.#7,. .083”.Diameter.1. Ball.Mill,.Carbide,.#1/4,. .019”.Diameter.1. Ball.Mill,.Carbide,.#1/2,. .027”.Diameter.4. Cutoff.Disk.1. Mandrel,.Screw.1. Mandrel,.Threaded.

Unit includes everything pictured



501274. Electrosurgical.Unit,.110V. US$851501285. Electrosurgical.Unit,.220V. US$909501273. Handpiece.for.electrodes,.Ø.1 .6mm.shaft. US$79501275.. Indifference.Plate. US$45501277. Footswitch. US$68501278. Diamond.Shape.Loop.Electrode,.Ø 1 .6mm.shaft. US$22501279. Small.Loop.electrode,.Ø 1 .6mm.shaft. US$22501280. Large.Loop.Electrode,.Ø1 .6mm.shaft. US$22501281. Fine.WIre.electrode,.Ø 1 .6mm.shaft. US$22501282. Wire.Electrode,.Ø 1 .6mm.shaft. US$22501283. Small.Oval.Loop.Electrode,.Ø 1 .6mm.shaft. US$22501284. Ball.Electrode,.Ø 1 .6mm.shaft. US$22501286. Set.of.7.Electrodes. US$115

501819 Micro.Drill.System,.110.V US$1,034

REPLACEMENT ACCESSORIES501850. Abrading.Tip,.Rubber,.pk.of.20. US$52501851. Abrading.Tip,.Stone,.pk.of.5. US$35501852. Accessory.Stand. US$12501853. Ball.Mill,.Carbide,.#1,. .031”.Diameter,.pk.of.5. US$45501854. Ball.Mill,.Carbide,.#2,. .039”.Diameter,.pk.of.5. US$45501855. Ball.Mill,.Carbide,.#3,. .047”.Diameter,.pk.of.5. US$45501856. Ball.Mill,.Carbide,.#4,. .055”.Diameter,.pk.of.5. US$45501857. Ball.Mill,.Carbide,.#5,. .063”.Diameter,.pk.of.5. US$45501858. Ball.Mill,.Carbide,.#6,. .071”.Diameter,.pk.of.5. US$45501842. Ball.Mill,.Carbide,.#7,. .083”.Diameter,.pk.of.5. US$45501860. Ball.Mill,.Carbide,.#1/4,. .019”.Diameter,.pk.of.5. US$45501861. Ball.Mill,.Carbide,.#1/2,. .027”.Diameter,.pk.of.5. US$45501862. Cutoff.Disk,.pk.of.20. US$52501863. Mandrel,.Screw,.pk.of.5. US$45501864. Mandrel,.Threaded,.pk.of.5. US$45



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Sterilizing TraysSterilizing Trays

Fine.surgical.instruments.are.too.valuable.not.to.protect ..WPI’s.sterilizing.trays.help.reduce.damage.from.everyday.use ..With.top.quality.structural.Integrity,.these.trays.are.ideal.for.the.handling.and.storage.of.all.standard.microdissection.and.surgical.instruments ..

●.GE’s.ULTEM®.resin.ensures.product.strength,.structural.integrity.and.extended.life.cycle .

● See-through.lids.make.it.easy.to.locate.the.right.instrument .

● Suitable.for.all.methods.of.sterilization.(autoclave,.steam,.dry.heat.and.chemical) .

● Unique.“grid”.design.in.base.makes.it.easy.to.install.the.silicone.finger.mat.required.to.protect.the.delicate.instruments .

Combination Sterilization Tray & Storage Case

501729

New Larger Size!



Stainless.steel.sterilization.case.. .with.silicone.holding.pads.

197.x.107.x.32.mm.. 14213. . . . . . . . . . . . . . US$84230.x.150.x.50.mm. 501934. . . . . . . . US$114

500252. Sterilizer.tray,.15.x.6.x.2.cm.(6.x.2 .5.x.0 .75.in .),.base,.silicone.mat,.lid. US$35500253. Sterilizer.tray,.19.x.10.x.2.cm.(7 .5.x.4.x.0 .75.in .),.base,.silicone.mat,.lid. US$60500254. Sterilizer.tray,.25 .5.x.15.x.2.cm.(10.x.6.x.0 .75.in .),.base,.silicone.mat,.lid. US$90501728 Sterilizer.Tray,.25 .5.x.15.x.4.cm.(10.x.6.x.1 .5.in .),.base,.insert.tray,.lid,.2.mats. US$207501729 Sterilizer.Tray.for.Knife.15.x.6.x.2.cm.(6.x.2 .5.x.0 .75.in .),.base,.lid,.2.bars. US$35 500255. Silicone.mat,.38.x.25 .5.cm.(15.x.10.in .). US$83

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Instrument Tip ProtectorsProtect your valuable instruments during

cleaning, sterilization, and storage Steam and gas sterilizableRadiopaque and latex-free

50 per pack

Instrument Portfolio

Aluminum Sterilizing Trays



501341. Tip.Protectors,.1/16”.x.3/4”.(1 .6.mm.x.19.mm),.size.1,.white. US$33501342. Tip.Protectors,.5/64”.x.3/4”.(2.mm.x.19.mm),.size.2,.blue. US$33501343. Tip.Protectors,.7/64”.x.3/4”.(2 .8.mm.x.19.mm),.size.3,.green. US$33501344 Tip.Protectors,.1/8”.x.1”.(3 .2.mm.x.25 .4.mm),.size.4,.red. US$33501345 Tip.Protectors,.3/16”.x.1”.(4 .8.mm.x.25 .4.mm),.size.5,.yellow. US$33 501346. Tip.Protectors,.13/32”.x.3/4”.(10 .3.mm.x.19.mm),.size.6,.maroon. US$33501347 Tip.Protectors,.1/16”.x.3/8”.x.1”.(1 .6.mm.x.9 .5.mm.x.25 .4.mm.),.size.7,.orange. US$33.501348 Tip.Protectors,.5/64”.x.5/8”.x.1”.(2.mm.x.16.mm.x.25 .4.mm),.size.8,.brown. US$33501349 Tip.Protectors,.1/8”.x.1”.x.1”.(3 .2.mm.x.25 .4.mm.x.25 .4.mm),.size.9,.black,.. US$33501340 Tip.Protectors,.Assorted.Pack.(equal.number.of.above,.plus.5). US$33

Store.your.instruments.in.style ..Elastic.bands.hold.instruments.in.place.against.soft.velveteen.fabric ..Portfolio.folds.in.half.and.zips.closed .

501319 US$52

These.sterilization.containers.are.made.of.hard.anodized.aluminum.and.can.withstand.autoclaving.and.ethylene.oxide.sterilizing ..The.non-perforated.base.has.two.locking.clasps.that.secure.the.lid.in.place ..The.perforated.lid.with.disposable.filter.allows.steam.and.gas.to.move.freely.in.and.out.of.the.container.during.sterilization ..A.silicone.mat.may.be.purchased.separately.and.cut.to.fit.the.base.of.the.tray ..

501913. Small.Aluminum.Tray,.300.x.140.x.40.mm. US$256 501914. Large.Aluminum.Tray,.300.x.140.x.70.mm. US$267501915. Single-use.Filters,.100.per.package. US$55501916. Indication.Labels,.100.per.package. US$68500255. Silicone.Mat,.38.x.25 .5.cm. US$83



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GERMINATOR SPECIFICATIONSO .D .. 17 .1.x.13 .3.x.12 .9.cm. . .. (63/4.x.51/4.x.51/16.in .)

I .D .. 5 .1.x.10 .2.cm.(2.x.4.in .)

WEIGHT. 2 .3.kg.(5.lb .)

Dry SterilizerSterilize.your.microdissecting.and.tissue.culture.instruments,.thor-oughly.and.conveniently,.in.seconds ..No.chemicals ..No.flames ..No.risk.of.burns ..No.disinfectant.fluids ..Glass.beads.heated.to.260°C.kills.all.viruses,.aerobic.and.anaerobic.bacteria,.yeasts.and.spores ..(1 .5.mm.lead-free.glass.beads.included .)

Pulsed Ultrasonic CleanerSeveral.times.more.powerful.than.similar.devic-es,.PUC.dislodges.dirt.left.behind.by.ordinary.ultrasonic.cleaners ..Although.very.compact.(the.well.is.8.cm.diameter.by.3 .5.cm.deep),.PUC.creates.maximum.turbulence.by.combining.a.subsonic.wave.and.a.high.intensity.ultrasonic.wave ..The.well.will.hold.small.microdissection.instruments ..Insert.the.plastic.cup.in.the.well.and.triple.the.volume.available.for.cleaning ..Dual.settings.for.normal.or.high.power ..Shuts.off.automatically.after.5.minutes .

Stainless.steel.tank.volume.is.half.a.liter ..Dura-ble.and.compact,.this.unit’s.robust.all-metal.con-struction.allows.for.continuous.duty ..Includes.lid .

Half-LiterUltrasonic Cleaner

PUC SPECIFICATIONSINPUT.POWER. 110V/220V,.60.Hz/50.Hz,.

70W

PEAK.OUTPUT. 60.kHz

WELL.CAPACITY. 60.mL.(20.oz .)

. . 8 .5.x.12.cm

CUP.CAPACITY. 175.mL

. . 8.x.3 .5.cm

O/D. 13.x.21.x.15.cm.

. . (51/8.x.81/4.x.47/8.in .)

SHIPPING.WEIGHT. 10.lb.(4 .3.kg)UBATH SPECIFICATIONS

INPUT.POWER. 22W

PEAK.OUTPUT. 70W,.55.kHz

TANK.CAPACITY. 0 .53L.(18.oz .)

TANK.I .D .. 12 .1.x.8 .6.x.6 .6.cm.. . (43/4.x.33/8.x.25/8.in .)

TANK.O .D .. 13 .7.x.10 .5.x.12 .1.cm.. . (53/8.x.41/8.x.43/4.in .)

SHIPPING.WEIGHT. 1 .8.kg.(4.lb .)

Ideal for small parts and microdissection

instruments



14404. Dry.Sterilizer.(110.V). US$477500121. Dry.Sterilizer.(220.V). US$59814405. Extra.Glass.Beads. US$16

UBATH-Y. Ultrasonic.Cleaner.(110.V). US$234UBATH-Z. Ultrasonic.Cleaner.(220.V,.Euro.plug).US$283UBATH-B.Ultrasonic.Cleaner.(220.V,.UK.plug). US$283


13740. Ultrasonic.Detergent.(4 lb). US$2814342. Ultrasonic.Detergent.(1 gal). US$45

PUC-Y. Pulsating.Ultrasonic.Cleaner US$11413740. Ultrasonic.Detergent.(4.lb). US$28

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Cidex PlusExcellent biocide for WPI’s nitric oxide sensors and other sensitive instruments which cannot be subjected to alcohol or autoclave

Cidex.Plus.achieves.a.high.level.of.disinfection.in.20.to.45.min-utes,.with.complete.sterilization.in.10.hours ..Custom.test.strip.allows.solution.to.be.tested.to.determine.if.minimum.effective.concentration.of.glutaraldehyde.is.present ..Non-corrosive.to.instruments ..Surfactant.enhanc-es.wetting.and.rinsing.proper-ties ..Mint.scent .

ENZOL Enzymatic DetergentENZOL.Detergent.is.a.mild.pH,.enzyme-based,.presoak-plus-clean-er.for.removing.tough,.dried-on.or.hard-to-reach.organic.matter.from.instruments.before.they.are.disinfect-ed.in.CIDEX.Solution ..The.proteolytic.enzymes.break.down.organic.mat-ter.in.one.easy.step ..Enzol.is.fast.—.results.begin.in.one.minute ..Pro-teolytic.enzymes.penetrate.and.break.down.protein,.reducing.the.need.for.mechanical.cleaning ..Effective.at.room.temperature.(no.need.for.hot.water) ..Safe.for.instruments,.including.delicate.endoscopes;.mild.pH.reduces.likelihood.of.corrosion ..Easy.to.rinse,.so.it.won’t.clog.scopes.or.leave.a.residue.on.instruments ..Low.foaming,.making.it.effective.for.use.in.automat-ed.equipment .

AlconoxPowdered Precision Cleaner

l..leaves.no.interfering.residues.on..............reusable.labware

l..use.with.manual.cleaning.or.ultrasonic

l..free.rinsing

l..corrosion.inhibited

Alconox.can.be.used.as.a.paste,.a.soak,.and.ultrasonically ..It.can.be.used.on.glass,.metal,.stainless.steel,.porcelain,.ceramic,.plastic,.rubber,.and.fiberglass ..It.can.also.be.used.on.soft.metals.such.as.copper.aluminum,.zinc,.and.magnesium.if.rinsed.promptly ..Passes.inhibitory.residue.test.for.water.analysis.and.is.authorized.by.the.USDA.for.use.in.federally.inspected.meat.and.poultry.plants ..

Used to remove: soil,.grit,.grime,.buffing.compound,.slime,.grease,.oils,.blood,.tissue,.salts,.deposits,.particulates,.solvents,.chemicals,.radioisotopes,.radioactive.contaminants,.silicone.oils,.mold.release.agents



7364. Cidex.Plus.(quart). US$20

Surgical Instrument LubricantProtect.your.surgical.and.dissecting.instruments.with.this.specially.formulated.lubricant .

500126. Surgical.Instrument.Lubricant.(8.fl.oz). US$16

Instrument DemagnetizerInstantly.magnetize.or.demagnetize.small.surgical.instruments.and.small.steel.parts.that.are.over.1 .3".in.length.and.1/8".in.diameter ..No.batteries.or.electricity.required ..Box.is.high.quality.molded.ABS.plastic,.impact.resistant,.and.cadmium.free ..Dimensions.are..2".x.2".x1".

501900. Instrument.Magnetizer/Demagnetizer. US$43.

7363. ENZOL.(quart). US$35

13740. Alconox.Cleaner.(4.lbs). US$28

503231. Micro.Cleaning.Brushes,.vial.of.100. US$28



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EpoxiesCarbon Epoxy (2 oz.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167Silver Epoxy (1 oz.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167

AdhesivesScotch-Weld 4886 (2 oz.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166Mini Glue Gun with hot melt glue sticks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167Vetbond Adhesive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168

Silicone AdhesivesNEW Kwik-Gard™ — Sylgard in a mixing applicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166Sylgard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166Kwik-Sil Silicone Adhesive Compound . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167RTV Adhesive Sealant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167RTV Coating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167

SealantsSylgard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166Kwik-Cast Silicon Casting Compound . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167RTV Sealant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167

PrimersRTV Prime Coat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167

“Super” AdhesivesCyanoacrylate adhesive, low viscosity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168Cyanoacrylate adhesive, high viscosity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168Octyl Cyanoacrylate adhesive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168

Specialty WiresCarbon (with or without PVC coating) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Hastelloy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Indium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Micro Coaxial Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Platinum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Platinum/Iridium (with or without Teflon coating) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Silver (with or without Teflon or PVC coating) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Stainless Steel (with or without Teflon coating) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Titanium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Tungsten . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Thermocouple Iron plus Constantan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171Thermocouple Chromel plus Alumel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171

AccessoriesBarbed Tubing Assortment Kit ( polypropylene) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172Cables and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174Chart Recorders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170Clamps & Bases, Non-Magnetic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 Digital Caliper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169Digital Micrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169Lab Coat, Medium Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168Luer-to-Tubing Coupler Assortment Kit (polypropylene) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172Luer-to-Tubing Coupler Assortment Kit (nylon) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172Luer Valve Assortment Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172, 173Magnetic Heating Stirrer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169Rack Mount Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178Screwdriver Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169Wire Cutters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169

Lab Supplies & Accessories



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Adhesives Application GuidePart No. Description Curing time Useful Applications and Characteristics Epoxies Formstrongbonding.Usedinwirebondingapplications. 4898 SilverfilledconductiveEpoxy 12hrs@50C;5min@150C Connectingconductorsthatcan'tbesoldered.ConstructingorconnectingAg/AgClpellets. 7335 CarbonfilledconductiveEpoxy 48hrs@25C;5min@150C Constructingcarbonelectrode. 4886 HighperformanceStructuralEpoxy 12hrs@25C. Formsastrongandslightlyflexiblebondonplastic,metal,andglass.Bondssomelowsurface. energyplasticsuchasPEEK Hot melt (EVA) Easytouseforbonding,needslargegapfilling 13316 MiniGlueGunwithgluesticks Assoonasitcoolsdown Bondswood,glass,metals,andmanyplastics. Silicone Adhesives/Sealants/Primers Goodmoistureresistantandelastic.Lowtoxic. 1571 Roomtemperaturevulcanizing (RTV)adhesive.Acyloxy/moisture curesystem.Aceticacidiscure by-product. 24hrs@25C Hasthebestadhesionpropertyinthissiliconefamily.Willbondtomanymaterials. 7128 RTVsealant.Alkoxy/Moisturecure system.Methanolascureby-product. 72hrs@25C Goodforbondingorsealingelectronicscircuits(metal). SYLG184 Sylgard,Twoparts,vinyl/platinum curesealant.Hydrogenascure by-products.Verylowtoxic 24hrs@25C,15min.@150C CoatingPatchClampelectrodes,Cellculturedish,makingdissectionpads. Kwik-Sil Twopart,adhesive.Vinyl/platinum system,Hydrogenascureby-products. Verylowtoxic. <5min@25C Livetissueandnervestudies.Mediumstrengthadhesion. Kwik-Cast Twopartsealant.Vinyl/platinum curesystem.Hydrogenascureby-products. Verylowtoxic. <5min@25C Sealantforlivetissues.Embeddingperipheralnerveswithelectrodes. 6820 Primerforsilicone N/A Enhancesadhesionofsiliconeadhesivesfordifficulttobondplasticsurfaces Cyanoacrylate Formsaninstantaneousbonding. 7341 EthylCyanoacrylate,lowviscosity 90-120cps <10seconds Mountingrat/mousebrainslices.Idealforrelativelysmallgapsandsmoothsurfaces.Bondsplastic,metalsand rubber.7342 EthylCyanoacrylate,highviscosity 1100-1600cps <30seconds Useonbrainsliceexp.Idealforlargergaps,allowsslightlylongerbondingtime.Bondsplastic,metalsand rubber.Vetbond ButylCyanoacrylate,Lowtoxic <10seconds Bondstissues,alternativetosuture,helpssmallwoundhealing.Antimicrobialeffect.Usedinforensicscience. 500280 OctylCyanoacrylate,Lowtoxic <15seconds Suitableforsurfacewoundbonding,protection,holdingasensororotherdeviceonthetissue.

Scotch-Weld 2216 Structural Epoxy

SylgardA two-part silicone elastomer, ideal for potting and encapsulat-ing applications. Very low dielectric constant sealing compound used in patch clamping and many other lab applications. After cure, will withstand -55° to 200 °C.

Shipping weight: 2 lb. (1 kg)

Kwik-Gard™

Kwik-Gard is specially packaged Sylgard 184 silicone for quicker and easier application, eliminating the messy procedure of preparing the mixture before application. Its special cartridge controls the precise mixing ratio to ensure proper curing. The disposable tip mixes resin and hardener as they are dispensed. Since no air is introduced during mixing, the resin does not need degassing for most applications. The mixed silicone is applied directly to the site, reducing preparation time and material waste.



Probably still the best epoxy for bonding plastic, often used as the benchmark for testing the binding strength of other adhesives. The slightly rubbery texture also makes it less easy to break off. It is the only epoxy known that can bond PEEK. Color: gray. Cures at room temperature.

Shipping weight: 1 lb. (0.5 kg)

4886 Scotch-Weld 2216 (2 oz.) US$56SYLG184 Sylgard (1.1 lb) US$114

Each Kwik-Gard cartridge contains 37 mL of resin and hardener. The dispensing tip has a dead volume of 0.75 mL.

KWIKGARD Kwik-Gard Start-up Kit (incl. dispenser, 1 cartridge, 5 tips) US$137

KWIKGLUE Kwik-Gard Refill (2 cartridges, 10 dispensing tips) US$79

KWIKMIX Dispensing Tips (pkg of 10) US$35KWIKGUN Kwik-Gard Dispenser US$114



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SElectrically Conductive Silver Epoxy

Electrically Conductive Carbon Epoxy

Kwik-Cast™ is a very low viscosity silicone elastomer sealant. It is able to fill small spaces around nerves and creates a continuous mass ensuring long-term recording stability. Kwik-Cast is color-coded so mixing is foolproof. It can be applied and cured underneath mineral oil. The slight longer curing time (approx. 3 minutes) makes it more suitable for stationary preparations and in vitro tissue studies.

Low toxicity 5-minute adhesives for live tissues!• Specially formulated for WPI

• Extremely low toxicity

• Excellent moisture resistance

• Cures at room temperature

The properties of this adhesive make it exceptionally useful for neuroscience applications, peripheral nerve studies and similar biomedical applications. These new silicone elastomers also eliminate the mess and time involved in pre-mixing other commonly used formulations (such as Wacker SilGel® and Sylgard®). Each silicone elastomer is packaged in a double barrel syringe and is automatically mixed when pressed out of the mixer tip provided. It can then be applied directly to the tissue without further mixing. The curing time of these silicones is short, reliable and reproducible thus eliminating potential costly guesswork when using other tissue adhesives. Furthermore, the curing process does not produce heat, which can otherwise cause tissue damage. WPI’s silicone elastomers are much less toxic than dental silicone because they contain no surfactant additives.

WPI’s silicone elastomers are based on recently developed vinyl terminated siloxane and platinum complex catalysts. They exhibit exceptional low toxicity before, during and after curing. The traditional RTV silicone systems produce either acetic acid or alcohol during condensation; these compounds are toxic to living cells. In contrast, the only by-product of condensation from WPI’s elastomers is a small amount of hydrogen gas, which was shown not to cause any effect on nerve activity in highly sensitive peripheral nerves. Both elastomers cure on contact with the tissue. Currently, WPI provides rapid-curing silicone elastomers in two different formulas:

Kwik-Sil™ is a translucent silicone elastomer adhesive with medium viscosity. It has good adhesion and mechanical property (tear strength and elongation). The very short curing time (approx. 1 minute) make it especially useful for moving preparations.

KWIK-CAST & KWIK-SIL SPECIFICATIONSUnmixed Kwik-Sil Kwik-Cast Mix Ratio 1:1 1:1

Working Time, typically 2-10 minutes 2-3 minutes

Viscosity, cps 15,000 10,000

After Curing 24 hours:

Tear Strength 90 ppi 44 ppi

Elongation 650% 60%

Durometer (shore A-2) 30 36

Color translucent green

Volume Resistivity, Ω/cm 1x101x10

Shipping Weight 0.5 lb 0.5 lb

References

Sivitz, et al., “Effect of acute and antecedent hypoglycemia on sympathetic neural activity and catecholamine responsiveness in normal rats.” Diabetes. 50:1119-1125, 2001.10 mixing tips

included

10 mixingtips included



Two-component silver-filled epoxy for electrical connections which cannot be soldered, such as Ag/AgCl pellets. This widely used silver-filled epoxy features low viscosity and smooth flowing charac-ter. Pure silver is dispersed in both resin and hard-ener. Cures in 15 minutes at 120 °C. Mix ratio 1:1. May be premixed and frozen for later use.


4898 Silver Epoxy (1 oz.) US$213

Two-component carbon-epoxy, curable at room and elevated temperatures. Ideal for electrostatic discharge protection and EMI/RFI shielding. 1:1 mix ratio. May be premixed and frozen for later use.


7335 Carbon Epoxy (2 oz.) US$109

Mini Glue GunComes with three sticks of special formula hot melt glue. UL approved. 110V 60Hz only.


13316 Mini Glue Gun US$20

Silicone RTV adhesiveClear silicone sealant provides good bonding to plastic. After cure, will withstand -55 to 200 °C. No mixing required. A handy, general purpose labora-tory sealant. (Releases acetic acid during curing.)


1571 RTV Sealant (4.7 oz.) US$29

Silicone RTV adhesive (non-acidic)Because it is non-corrosive, this material is ideal for use on metal, for encapsulating small circuits on connectors. After cure, will withstand -55° to 200 °C. No mixing required.


7128 RTV Coating (3 oz.) US$56

RTV Prime CoatEnhances adhesion of silicone adhesives to many difficult-to-bond plastic surfaces.


6820 RTVPrimeCoat,400ml(13.5oz.) US$52

KWIK-SIL Silicone Adhesive Compound (two 5 mL syringes) US$98KWIK-CAST Silicone Casting Compound (two 5 mL syringes) US$86600009 Replacement Mixing Tips (pkg of 10) US$33

PRICE BREAK 1-5 pkg 6-9 pkg More than 10KWIK-CAST US$86 US$79 US$69KWIK-SIL US$98 US$91 US$81

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S“Super” Adhesives for Life Science ResearchCyanoacrylate adhesives have been on the market since 1958. Most industrial or household grade cyanoacrylate is made of shorter alkyl chain derivatives such as methyl or ethyl cyanoacrylate (WPI’s #7341 and #7342). They are very useful for temporarily holding tissues such as mounting specimens for microtome sectioning. However, they are not suitable for bonding wounds on live animals. The difficulties of using cyanoacrylate for bonding live animals are: (1) a strong, irritating odor; (2) quick loss of bonding strength due to breakdown of the bonding by hydration; (3) the breakdown products, cyanoacetate and formaldehyde, are toxic and can cause inflammatory reactions; and (4) they have low flexibility and tend to be brittle.

To overcome these problems, several longer alkyl chain cyanoacrylates have been developed especially for veterinary and human use. The first longer alkyl chain product is butyl cyanoacrylate. This product has been used for animal and human applications outside the USA since 1970. It is much less toxic and has a lower odor than the methyl or ethyl cyanoacrylate. The butyl cyanoacrylate offered by WPI is Vetbond™.

A family of adhesives containing octyl cyanoacrylate, a plasticizer and stabilizer, was developed In the 1990’s (one of them approved by FDA for human use). When bonding to tissue, these new adhesives are four times stronger and less toxic than butyl cyanoacrylate. Compared with the traditional suture, the new super adhesive has several advantages. On average, it takes only one-tenth of the time to close an incision. The bonding strength is equal to 5-0 monofilament suture. It also has a mysterious antimicrobial effect that can decrease infection rates in contaminated wounds. Bonding will slough off naturally in 5 to 7 days. Cosmetic appearance of the healed incision is also better.

Octal cyanoacrylate adhesive #500280 forms a strong and flexible film and is thus more suitable for surface wound bonding, protection, and holding a sensor or other device on the tissue. Setting time is about 10 seconds, which gives ample time for application. It can also be used for temporarily holding a live tissue. For example, there is a report of using it to hold nematodes on a glass slide for patch-clamp neurons recording.

All of the products offered by WPI are veterinary grade (not suitable for human application). Though very similar to the grade for human use, they are not sterile and do not have FDA approval.

Lab CoatLight weight, medium length — for the lab professional

Pulsed Ultrasonic CleanerSeveral times more powerful than similar devices, PUC dislodges dirt left behind by ordinary ultrasonic cleaners. Although very compact (the well is 8 cm diameter by 3.5 cm deep), PUC creates maximum turbulence by combining a subsonic wave and a high inten-sity ultrasonic wave. The well will hold small microdissection instruments. Insert the plastic cup in the well and triple the volume available for cleaning. Dual settings for normal or high power. Shuts off automatically after 5 minutes.

PUC SPECIFICATIONSINPUT POWER 110V/220V, 60 Hz/50 Hz, 70W

PEAK OUTPUT 60 kHz

WELL CAPACITY 60 mL (20 oz.) 8.5 x 12 cm

CUP CAPACITY 175 mL 8 x 3.5 cm

O/D 13 x 21 x 15 cm (51/8 x 81/4 x 47/8 in.)

SHIPPING WEIGHT 10 lb (4.3 kg)

Ideal for small parts and microdissection

instruments

Available in five sizes:



500280 Octyl Cyanoacrylate Adhesive, 1.5 mL US$567341 CyanoacrylateAdhesive,lowviscosity—90-120cps(pkgof10) US$407342 CyanoacrylateAdhesive,highviscosity—1100-1600cps(pkgof10) US$40VETBOND 3M Vetbond™ Adhesive (3 mL) US$29

PUC-Y PulsatingUltrasonicCleaner US$9913740 UltrasonicDetergent(4lb) US$28

LABCOAT-XS Lab Coat, Extra Small US$29LABCOAT-S Lab Coat, Small US$29LABCOAT-M Lab Coat, Medium US$29LABCOAT-L Lab Coat, Large US$29LABCOAT-XL Lab Coat, Extra Large US$29

Quantity discount available



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Wire Cutters Screwdriver Set

Digital Micrometer

Digital Caliper

Magnetic Heating Stirrer

Digital Caliper & Micrometer

The Magnetic Heating Stirrer enjoys the advantages of convenience, stable and steady operation as well as indefinite speed regulation. It can carry out stable and precise agitation of solutions in a relatively common temperature range. It is especially suitable for agitation of small volume samples.

Specifications

Speed Range 0-1400 rpm

Tray Diameter 12 cm

Heating Power 100 W

Power Supply AC 110 V / 50 Hz

Dimensions 23 x 16 x 10 cm

Stirring Power 3 - 6 W

Temperature 20°C - 210°C

This production grade precision 5-piece screwdriver set is the highest quality tool you can find on the market. Made by German craftsmen, the chrome-vanadium tips will fit any screw securely without leaving marks. The set contains an ESD safe handle with 8 interchangeable blades. Phillips Sizes: 000, 00, 0, 1. Slotted Sizes: 1.5, 2.0, 3.0, 3.5 mm. The 000 size Philips blade is the smallest you can find any-where — it can fit smallest screw on a 35 mm camera. Weight: 0.24 lb

Notched blade

12cm long

The high quality electronic digital caliper and micrometer are useful tools — no laboratory should be without one because they are more accurate and easier to use than the traditional analog units. Measure in either inches or millimeters at the touch of a button. The floating zero feature allows you to read the increment without calculation. Both of them have the SPC output for interface with external readout devices. The caliper is made from hardened stainless. The micrometer meets the IP54 as defined in the IEC529 standard that is resistance to dust and splashing water. The caliper measures up to 150 mm (6 inch) with 0.01 mm (or 0.0005") resolution. The micrometer measures up to 25 mm (or 1 inch) with 0.001 mm (or 0.00005") resolution.

The micrometer is designed to be water-resistant to IP54 as defined in the IEC529 standard. The IP54 code’s first digit “5” means dust-protected. The second digit “4” means that the caliper is protected against splashing water — it has to withstand a shower from every direction for 10 minutes.

Computer Link — Adapter cable SPG-KIT allows direct communication between micrometer (or caliper) and computer through serial port. Press button on cable to transmit data to computer. The SPG-KIT comes with software which enters the reading into any Windows application as if entered with the keyboard. Data Rate: 4800 bit/s. Data Format: 1 start bit, 8 data bits, 1 stop bit.

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501601 Digital Caliper US$75

503036 Digital Micrometer US$148

502157 Replacement Battery (package of 10) US$35

501610 WPI-02 Magnetic Heating Stirrer US$229

501321 Roger Wirecutting Scissors US$23

501635 Professional Quality Screwdriver Set US$56

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170

The BD11E single-channel and BD12E dual-channel flatbed recorders have proven ideal in research, development and production, in service roles and in education. The slim wedge-shaped chart bed presents just the right angle for adding notes to tracings. Electric pen lifts respond automatically to a 30-second pause in activity, which prevents pens from bleeding.

Home and Grid Functions—The home function lets you reposition the recorder pen at the start of the plot, which aids in replotting, data analysis, and in taking comparative traces. The grid function advances the pen to the next grid line, which aids in repetitive measurements and ensures that subse-quent traces share a common starting point.

CHART RECORDER SPECIFICATIONS Kipp & Zonen

Inout ranges 1 mV...20 V (14 steps) Differential

Inout impediance 1 MΩ DC

10k in series with 1.5 µF for AC

Zero Offset 1.5x Full Scale Commin Mode Rejection 130 dB

Linearity ±0.3% Full Scale

Chart Speeds 0.1 to 20 mm/sec 0.1 to 20 mm/min

Response Time < 0.2 seconds for 5 to 95% FSD

Chart Paper 25 m rolls, according to DIN 16230 4100 hrs at lowest speed 20 minutes at highest speed

Power 95-135 V or 220-240 V, 50/60 Hz

Chart Drive Functions Fast forward & reverse, grid, home, event marker, polarity reversal switches, external control via 15-pin interface

Kipp & Zonen Chart Recorders

Work-enhancing features plus proven performance



BD11E Chart Recorder, single pen w/ pen lift & remote control BD12E Chart Recorder, dual pen w/ pen lift & remote control


OPTIONAL ACCESSORIESBDXR9 Chart Paper (10 rolls) BDXR124 Black Pens, BD11 & BD12, Ch. 1 (pkg of 6) BDXR13 Red Pens, BD12, Ch. 2 (pkg of 6) BDXR13B Black Pens, BD12, Ch. 2 (pkg of 6)



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171

Precious Metal and Specialty Wire

New! Micro coaxial cables (MAXxxxx) are ideal for microelectrode fabrication and construction of similar research tools. The dual shielding eliminates electrical interference caused by radio frequencies (RF), electrostatic and microphonics (e.g., bending and vibration. Available with single or dual (twin) conductors.

Thermocouple wire for temperature monitor-ing. TCJ3050, with an iron/constantan pair of wires, can be used in inert, vacuum, reducing or oxidizing atmospheres (iron conductor may rust in moist or low temperature applications). TCK3050 has a chromel/alumel pair, for use in inert or oxidizing atmosphere at 1500-1800 °F.

Teflon-coated stainless steel (type 304) wire (SSTxxxx) is available in 25-ft and 50-ft lengths. The Teflon coating is 150 micro-in. thick (4 µm).

Carbon wire (C3005) is a single 30-micron fiber of electrochemically activated carbon. This fiber is especially useful in micro-electro-chemical experiments.

Carbon filament wire (CPVCxxxx) is made of 3,000 microscopic carbon strands in a col-ored PVC jacket. 45.8 ohms/foot.

Copper ultra-fine wire (CU7T44G) is made of seven individually insulated 44 AWG (50 micron) conductors twisted together. Total diameter with insulation is 200 microns. Resistance: 2.9 Ω/m for all seven wires together. Dry dielectric strength: 1500V DC.

Platinum/iridium wire — uncoated (PTxxxx) and Teflon-coated (PTTxxxx) — is an alloy of 90% platinum and 10% iridium, giving excellent tensile strength and corrosion resistance. Uncoated pure platinum wire (PTPxxx) is 99.95% pure.

Indium wire (IN1003) is 99.99% pure, with a melting point of 156.4°C.

Annealed silver wire (AGWxxxx), 99.99% pure, is available in five diameters; three of those sizes are also available with a Teflon coating (AGTxxxx), and the 0.010-in. wire can be ordered with a PVC coating (AGPVCxxxx).

Tungsten wire (TGWxxxx), available in three diameters, is 99.95% pure.

Gold wire (AUWxxxx) is 99.99% pure.Titanium wire (TIxxxx) is 98.9% pure,

annealed, in three diameters.Stainless steel wire (SSxxxxx) is type 316.

Micro Coaxial Cables

0.61 mm

!



Catalog No. Metal Coating AWG* Diameter Precut Length PriceAGT0510 Silver Teflon 36 0.005in.(0.125mm)1 10ft(3m) US$45AGT0525 Silver Teflon 36 0.005in.(0.125mm)1 25ft(7.6m) US$102AGT05100 Silver Teflon 36 0.005in.(0.125mm)1 100ft(30m) US$316AGT1010 Silver Teflon 30 0.010in.(0.25mm)1 10ft(3m) US$52AGT1025 Silver Teflon 30 0.010in.(0.25mm)1 25ft(7.6m) US$86AGT10100 Silver Teflon 30 0.010in.(0.25mm)1 100ft(30m) US$167AGT1510 Silver Teflon 26-27 0.015in.(0.38mm)1 10ft(3m) US$86AGT1530 Silver Teflon 26-27 0.015in.(0.38mm)1 30ft(9.1m) US$137AGW0510 Silver — 36 0.005in.(0.125mm) 10ft(3m) US$29AGW0530 Silver — 36 0.005in.(0.125mm) 30ft(9.1m) US$63AGW1010 Silver — 30 0.010in.(0.25mm) 10ft(3m) US$52AGW1030 Silver — 30 0.010in.(0.25mm) 30ft(9.1m) US$79AGW1510 Silver — 26-27 0.015in.(0.38mm) 10ft(3m) US$86AGW1530 Silver — 26-27 0.015in.(0.38mm) 30ft(9.1m) US$127AGW2010 Silver — 24 0.020in.(0.5mm) 10ft(3m) US$79AGW2030 Silver — 24 0.020in.(0.5mm) 30ft(9.1m) US$114AGW4010 Silver — 18 0.040in.(1.0mm) 10ft(3m) US$127AUW0170 Gold — 50 0.001in.(0.025mm) 70ft(17.7m) US$201AUW201 Gold — 24 0.020in.(0.5mm) 1ft(30cm) US$102C3005 Carbon — 49 0.0012in.(30µm) 5ft(1.5m) US$56CPVC4050Y Carbon PVC(yellow) 18 0.040in.(1.0mm) 50ft(15m) US$69CPVC4050G Carbon PVC(green) 18 0.040in.(1.0mm) 50ft(15m) US$69CPVC4050R Carbon PVC(red) 18 0.040in.(1.0mm) 50ft(15m) US$69CPVC4050W Carbon PVC(white) 18 0.040in.(1.0mm) 50ft(15m) US$69CU7T44G CopperAlloyNylon/Polyurethane 30 0.010in.(0.20mm) 16.5ft(5m) US$63IN1003 Indium — 30 0.01in.(0.25mm) 3ft(92cm) US$86PT1002 Platinum/Iridium — 30 0.010in.(0.25mm) 2ft(61cm) US$213PT0402 Platinum/Iridium — 38 0.004in.(0.102mm) 2ft(61cm) US$201PT0203 Platinum/Iridium — 44 0.002in.(0.051mm) 3ft(92cm) US$213PT0303 Platinum/Iridium — 40 0.003in.(0.076mm) 3ft(92cm) US$201PT0110 Platinum/Iridium — 50 0.001in.(0.025mm) 10ft(3m) US$293PTP101 Platinum — 30 0.010in.(0.25mm) 1ft(30cm) US$173PTP201 Platinum — 24 0.020 in. (0.5 mm) 1 ft (30 cm) US$184PTP401 Platinum — 18 0.039in.(1.0mm) 1ft(30cm) US$518PTP406 Platinum — 18 0.039in.(1.0mm) 0.5ft(15cm) US$224PTT0502 Platinum/Iridium Teflon 36 0.005in.(0.125mm)1 2ft(61cm) US$144PTT0402 Platinum/Iridium Teflon 38 0.004in.(0.102mm)1 2ft(61cm) US$293PTT0203 Platinum/Iridium Teflon 44 0.002in.(0.051mm)1 3ft(92cm) US$299PTT0110 Platinum/Iridium Teflon 50 0.001in.(0.025mm)1 10ft(3m) US$328SS31605 StainlessSteel — 36 0.005in.(0.125mm) 50ft(15.2m) US$45SS31614 StainlessSteel — 27 0.014in.(0.36mm) 30ft(9.1m) US$45SST30407-25 StainlessSteel Teflon 33 0.007in.(0.18mm)3 25ft(7.6m) US$79SST30407-50 StainlessSteel Teflon 33 0.007in.(0.18mm)3 50ft(15.2m) US$127TCJ3050 Thermocouple Glassbraid 30 50ft(15.2m) US$75 (Iron+Constantan)TCK3050 Thermocouple Glassbraid 30 50ft(15.2m) US$75 (Chromel+Alumel)TI2051 Titanium — 24 0.020in.(0.5mm) 24ft(7.3m)2 US$316TI4024 Titanium — 38 0.040in.(1.0mm) 24ft(7.3m)2 US$102TGW0325 Tungsten — 40 0.003in.(0.075mm) 25ft(7.6m) US$79TGW0515 Tungsten — 36 0.005in.(0.125mm) 15ft(4.6m) US$79TGW1510 Tungsten — 26-27 0.015in.(0.38mm) 10ft(3m) US$79

Microcoaxial CablesMAX3820 TinnedCuAlloy Coaxial 0.0173in.(0.44mm) 20ft(6m)4 US$102MAX4020 TinnedCuAlloy TwinCoaxial0.0158x0.024in.(0.4x0.61mm) 20ft(6m)5 US$114

*Brown & Sharpe1 Plus 0.002 in. for Teflon coating 2 Furnished in bundles of 3-ft lengths.3 Teflon adds 0.00015 in. (4 µm) to diameter 4 Impedance: 50 ohm; capacitance: 95 pF/m; resistance: 5 ohm/m5 Impedance: 100 ohm; capacitance: 54 pF/m; resistance: 1.9 ohm/m

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Luer Valve Assortment Kit

Luer-to-Tubing Coupler Assortment Kit Assemble quick-disconnect luer fittings for use with flexible tubing with internal diam-eters of 1⁄16", 3⁄32", 1⁄8" and 5⁄32". A variety of quick-disconnect connectors can be quickly made for connecting small diameter flexible tubing; 3-way connections can be made with the use of the 3-way luer tee; luer plugs, tees, connectors, bulk-head mounts, color coding rings, locking nuts, male and female luers—are all included to enhance the versatility of this kit. The kit has over 350 assorted parts and is offered in two different types of materials. Polypropylene fittings are chemically inert and resistant to most organic and inorganic solvents. Nylon fittings are strong and can be bonded with adhesive.

172

A useful kit (above) for building your own liquid flow experiment. It provides the means to start, stop, add, divide and control a flow of liquid or gas. Included in the kit are over 200 assorted parts such as one-way and three-way stopcocks, manifolds, Y-connectors, injection sites, male and female luer caps, check valves, syringe-activated check valves, slide clamps, roller clamps, and pinch clamps. All (except clamps) have a luer fitting for quick and easy connecting and disconnecting. Includes assorted luer fittings for use with flexible tubing.

Includes three tubing sizes: 1/16" ID, 1/8" ID, 1/4" ID



14012 Luer-to-Tubing Coupler Assortment Kit (Polypropylene) US$190500895 Luer-to-Tubing Coupler Assortment Kit (Nylon) US$190

Barb-to-Tubing Coupler Assortment KitBarb-to-Tubing Assortment Kit (at left) includes three different sizes of tubing and two boxes with different fittings, T-connectors, elbow connectors, check valves and plugs.

500890 Barb-to-Tubing Assortment Kit (polypropylene) US$190

14011 Luer Valve Assortment Kit US$305



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Luer Valve Assortment KitPN 14011 has over 350 assorted parts and is offered in two different types of materials

173

Parts now sold individually

Parts in kit may differ slightly in appearance from those pictured.



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174

Cables and Connectors #1358

#2851

#3161 #3294

#3417-10

#3491

#3492

#3508

#3517

#3670

#5371

#5372

#5373

#5375

#5374

#5385

#13388

#13776

#13451

#13620

#13347

#13324

#13854

#15976

#15975 #14254

#500081

#500082

#500128

#500131

#3578

#300102

#300040

#500256

#13685

#15623 #15676

#3142





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#CBL100 #CBL102 #15624



PART # APPLICATION/DESCRIPTION CONNECTOR A CONNECTOR B CABLE PRICE LENGTH1358 Beetrodes BNC(male) 2mmpin 3ft(0.9m) US$452026-10 2mmsocket,unwired(pkgof10)(NotShown) 2mmsocket unwired none US$242851 StandardBNCcable BNC(male) BNC(male) 6ft(1.8m) US$303142 Mini-BananaAdapter ScrewTerminals DualMini-Banana none US$253161 ConnectorforinputtoTBM4MandBP-1 DIN(male) unwired none US$233294 GroundwireforDAM80probe Clip none 3ft(0.9m) US$63417-10 2mmplug,unwired(pkgof10) 2mmpin unwired none US$243491 Extensionforany8-pinDIN DIN(male) DIN(female) 5ft(1.5m) US$1123492 Connector,adaptsWPItransducerstonon-WPIequipment DIN(female) unwired none US$213508 AdaptsBNCpHelectrodetopHmeterwith “U.S.Standard”input BNC(male) USStandard none US$683517 DAM50,DAM60,DAM70,shielded(twocables/pkg) Modularphoneplug,4wire none 3ft(0.9m) US$793578 AdaptercableforAg/AgClpellets 2mmpin none 5ft(1.5m) US$313670 Doublebananaplugwithsolderturretterminals DualBanana(male) DualBanana(female) none US$155371 Low-noisecableformicroelectrodeholders 2mmgoldpin 2mmgoldpin 2ft(0.6m) US$215372 Low-noisecableformicroelectrodeholders 2mmgoldjack 2mmgoldjack 2ft(0.6m) US$225373 Low-noisecableformicroelectrodeholders Combinationpin/jack Combinationpin/jack 2ft(0.6m) US$245374 Low-noisecableformicroelectrodeholders BNC(male) 2mmgoldpin 4ft(1.2m) US$405375 Low-noisecableformicroelectrodeholders BNC(male) 2mmgoldjack 4ft(1.2m) US$405385 Cable,shieldedtransducerstock none none 25ft(7.6m) US$12413324 Derm-Trode,Iso-Millivoltmeter Double-banana(female) BNC(male) none US$2813347 ISO2(chartrecorderadapter) Double-banana(male) BNC(female) none US$2913388 ElectrodeadapterforDAMprobes Miniaturebanana(male) 2mmjack none US$4713451 Adapter:Iso-DAM,Iso-DAM8 BNC(female) two2mmpins 6in.(15cm) US$2413555 SerialCable(notshown) DB9(male) DB9(female) 6ft(1.8m) US$3913620 Low-noisecableformicroelectrodeholders 2mmgoldpin 2mmgoldjack 2ft(0.6m) US$2213685 SPSeriespump-to-pumplinkingcable Modularphoneplug Modularphoneplug 7ft(2.1m) US$1513776 AdaptsreferenceelectrodetoVF4groundjack Banana(male) 2mmjack none US$1513854 BNCT-connector,maleto: BNC(female) BNC(female) none US$1514254 BNCStraightAdapter BNC(female) BNC(female) none US$1515623 Serialcable,SPSeriespump SPPump IBM9-pin“D”connector 5ft(1.5m) US$3915624 Serialcable,SPSeriespump SPPump Macintoshconnector 5ft(1.5m) US$3915676 Serialcable,SPSeriespump SPPump IBM25-pin“D”connector 5ft(1.5m) US$3915975 Adapter 2mmsocket 1mmpin none US$1515976 Adapter 1mmsocket 2mmpin none US$15300040 AdapterExtension 2mmsocket 2mmsocket 4in.(10cm) US$79500081 AdapterIBMPCCommPort DB9(male) DB9(female) none US$21500082 AdapterBNC/RCAMM BNC(female) RCA(male) none US$9300102 AdapterExtension 2mmsocket 0.31socket 4in.(10cm) US$95500128 BNCZeroOhmTerminator BNC(male) none none US$6500131 CableforBiode®SnapElectrodes(pkgof5) 2mmpin snap 5ft(1.5m) US$29500184 StandardBNCCable BNC(male) BNC(male) 10ft(3m) US$32500256 BNCRightAngleAdapter BNC(male) BNC(female) none US$15500257 StandardBNCCable BNC(male) BNC(male) 6in.(15cm) US$22500258 StandardBNCCable BNC(male) BNC(male) 12in.(30cm) US$22500259 StandardBNCCable BNC(male) BNC(male) 18in.(46cm) US$22501670 CoaxialAdapter DualMini-Banana(male) BNC(female) none US$63CBL100 MiniPhonePatchCable 3.5mmMiniPhoneplug 3.5mmMiniPhoneplug 6ft(1.8m) US$16CBL102 DAMSeries,PMSeries 3.5mmMiniPhoneplug BNC(male) 6ft(1.8m) US$32

POWER CORDS3006 US120V,grounded IECConnector 3-pin(M)US 6ft(1.8m) US$213301 European240V,grounded IECConnector 3-pin(M)European 6ft(1.8m) US$263302 British 240 V, grounded IECConnector 3-pin(M)UK 6ft(1.8m) US$3214088 Australian240V,grounded IECConnector 3-pin(M)Australian 6ft(1.8m) US$26

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176

Non-Magnetic Bases,Stainless Steel Rods, & Clamps

These high quality components are made of stainless steel and polymer that resist organic solvents and corrosion. They can be easily assembled to make a stand-alone setup for student labs or to make a complicated frame for research labs.

43 mm



503081-4 Vertical Surface Mount, M8 Threaded .............................. (including three 10-32 mounting screws) (pkg of 4)

503085 Large V-base with M8 Thread Mount

503084 Small V-base with M8 Thread Mount

502190 Heavy Rectangular Base (with M8 thread mount ................... and thumbscrew mount), 23×15.6 cm, 4 lb

503083 Light Rectangular Base (with M8 thread mount ...................... and thumbscrew mount), 23×15.6 cm, 0.5 lb

503088-10Thumbscrew, M5×20 mm (pkg of 10)

503070 Polished Stainless Steel Post, 12mm OD, 25cm long, no thread503071 Polished Stainless Steel Post, 12mm OD, 50cm long, no thread503072 Polished Stainless Steel Post, 12mm OD, 75cm long, no thread503073 Polished Stainless Steel Post, 12mm OD, 25cm long, M8 thread503074 Polished Stainless Steel Post, 12mm OD, 50cm long, M8 thread503075 Polished Stainless Steel Post, 12mm OD, 60cm long, M8 thread503076 Polished Stainless Steel Post, 12mm OD, 75cm long, M8 thread503077 Polished Stainless Steel Post, 12mm OD, 80cm long, M8 thread



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177

38.7 mm



14073-4 Open-sided Frame Clamp (pkg of 4)

503082-4 Board Frame Clamp, opens up to 8.5 mm (pkg of 4)

502193-4 Parallel Frame Clamp (pkg of 4)

503078-4 T-joint Frame Clamp (pkg of 4)

503080-4 Frame Clamp with Parallel Surface Mount (including two 10-32 mounting screws) (pkg of 4)

503079-4 In-line Frame Clamp (pkg of 4)

503041Large Clamp with Rod (157 mm), opens up to 85 mm

503042Medium Clamp with rod (157 mm), opens up to 45 mm

503086 Small Clamp with rod (157 mm), opens up to 16 mm

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Rackmounting HardwareMany instruments may be mounted in standard 19-inch instrument racks with the appropriate rackmount kit, as noted on the page featuring the instrument.

DAM Series amplifiers and Iso-DAM amplifiers may be mounted to the rack panels above by fastening bolts (included) through holes in the panel.

Dual rackmount kits allow many smaller instruments to be joined by bolting the chassis together and mounting the pair into a standard rack. (Pictured: #2932)

Brackets for instruments which are less than 17.5 inches wide have “wings” which extend to the standard rack width. (Pictured: #13024)



2932 Rackmount Kit, 31⁄2-in. high US$114

3468 Dual Rackmount Kit (705, A320, A362, A382, A385) US$114

2933 Rackmount Kit, 51⁄4-in. high US$114

13025 Dual Rackmount Kit (TBM-4 & BP-1) US$114

2935 Rackmount Kit, 8 3⁄4-in. high US$114

3484 Rackmount Kit for DAM Series amplifiers US$114

5381 Rackmount for Iso-DAM US$114

‑ 800283 Rackmount Kit with Handles US$114

13024 Single Rackmount Kit (TBM-4 & BP-1) US$114

3469 Single Rackmount Kit (705, A320, A362, A382, A385) US$114



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Sharp Electrode Glass CapillariesFire-Polished Single-Barrel Standard-wall and Thin-wall glass Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180

WPI’s Fire-polished glass capillaries are perfect for recording electrodes. The fire-polished ends eliminate

possible damage to the chloridized (Ag/AgCl) wire and microelectrode holder, and also facilitate smoother

insertion into a microelectrode holder.

Single-Barrel Standard Wall Capillaries (with or without inner filament) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180WPI’s standard wall capillaries are easier to pull longer and smaller tip micropipettes. These are particularly

suitable for intracellular microelectrodes. The inner filament greatly facilitates pipette filling with electrolyte.

Single-Barrel Thin Wall Capillaries (with or without inner filament) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180Thin wall capillaries produce smaller OD and bigger ID pipettes that are suitable for microinjection

Patch Clamp Glass CapillariesBest for patch clamp studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181

Multi-Barrel Glass CapillariesMulti-Barrel Standard wall tubing (with inner filament) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182

Designed for microiontophoresis

Septum Theta Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182Ideal for making dual-channel micropipettes used for intracellular work in small cells

Piggyback Glass Capillaries (with filament) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182Ideal for making two-barrel electrodes with a significant tip diameter differential

Borosilicate Glass Rod . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182Suitable for making rod pipettes to be used as tools to dissect chromosomes or to probe and move objects

Five- and Seven-Barrel Micropipette Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183 Designed for precise local application of chemicals in extracellular electrophysiological studies

µTip Pre-Pulled Glass CapillariesBorosilicate Glass Micropipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184

Plain, luer and silanized µTips

Microfil, Micropipette Holders and AccessoriesMicroFil Nonmetallic Syringe Needles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185

For filling micropipettes

Micropipette Storage Jar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183For 1.0mm, 1.2mm, 1.5mm and 2.0mm OD glass pipettes

PolyFil Multi-Barrel Micropipettes Coupling Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185For easy and secure coupling of a multi-barrel micropipette to a pressure source

Micropipette Holders & Half-Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 For coupling fluid-filled glass micropipettes to high input impedance amplifiers

Optical Quality Glass Bottom Sterile Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76 For microinjection, fluorescence, high resolution image analysis, cell culture, electrophysiology

Microelectrodes Metal Microelectrodes For extracellular recording . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Dri-Ref™ Reference Electrodes featuring extremely low electrolyte leakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

CALBUF Calcium Calibration Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

BetterSkin™ Electrodes Reusable surface-mounted electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190

Disposable Ag/AgCl Snap Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190

GEL100 Conductive Electrode Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190

Ag/AgCl Half-Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191

Glass, Holders & Electrodes



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Sharp Electrode Glass CapillariesClean, high quality glass for making micropipette electrodes and other research implements Standard,

no FilamentStandard,

with Filament

Thin Wall, no Filament

Thin Wall, with Filament

WPI offers a wide spectrum of high-quality glass capillaries. We take pride in our ability to ship your glass order within 48 hours. If you need a special glass that does not appear in our catalog, please call us. We will make every effort to provide it for you.

Fire-Polished glass capillaries are easier to insert into microelectrode holders without damaging the gasket. More importantly, fire-polished glass won’t scratch the chloridized wire used in a recording electrode. Fire-polishing does not affect the glass’s mechanical or electrical properties.

Borosilicate glass capillaries: Close dimen-sional tolerances assure microelectrode uniformity and reproducibility. Available in one-, two-, three-, five- and seven-barrel configurations; a complete range of single barrel thin-wall sizes; and a variety of special configurations. Capillaries with filaments contain a solid filament fused to the inner wall, which speeds filling of electrodes. Capillaries with or without inner filaments are available for making microelectrodes in a wide range of diameters .

Single Barrel standard wall thickness capil-laries are offered either with or without inner fila-ments for quick filling in a variety of lengths and diameters. Two usable electrodes can be made from one 6-inch length.

Thin Wall single barrel capillaries are offered both with or without inner filaments. The concen-tricity of this material provides excellent strength. Micropipettes made from thin wall capillaries have fine tips with a short taper.

Note: Because electrode tips erode when left filled with saline solutions for long periods, electrodes should be made and filled immediately prior to use.



Single-Barrel Standard 7740 Borosilicate Glass Tubing

LengthOD

(mm)ID

(mm)Filament

Fire-Polished

Quantity Item Price

3 in. (76 mm) 1.0 0.58 4 500 1B100F-3 US$473 in. (76 mm) 1.0 0.58 500 1B100-3 US$383 in. (76 mm) 1.2 0.68 4 350 1B120F-3 US$473 in. (76 mm) 1.2 0.68 350 1B120-3 US$383 in. (76 mm) 1.5 0.84 4 225 1B150F-3 US$53

3 in. (76 mm) 1.5 0.84 4 300 1B150-3 US$53

4 in. (100 mm) 1.0 0.58 4 4 500 1B100F-4 US$53

4 in. (100 mm) 1.0 0.58 4 500 1B100-4 US$44

4 in. (100 mm) 1.2 0.68 4 4 400 1B120F-4 US$534 in. (100 mm) 1.2 0.68 350 1B120-4 US$384 in. (100 mm) 1.5 0.84 4 4 300 1B150F-4 US$59

4 in. (100 mm) 1.5 0.84 4 300 1B150-4 US$53

4 in. (100 mm) 2.0 1.12 4 125 1B200F-4 US$47

4 in. (100 mm) 2.0 1.12 4 200 1B200-4 US$51

6 in. (152 mm) 1.0 0.58 4 500 1B100F-6 US$706 in. (152 mm) 1.0 0.58 500 1B100-6 US$646 in. (152 mm) 1.2 0.68 4 350 1B120F-6 US$646 in. (152 mm) 1.2 0.68 350 1B120-6 US$646 in. (152 mm) 1.5 0.84 4 225 1B150F-6 US$766 in. (152 mm) 1.5 0.84 225 1B150-6 US$706 in. (152 mm) 2.0 1.12 4 125 1B200F-6 US$646 in. (152 mm) 2.0 1.12 125 1B200-6 US$64

Thin-Wall Single-Barrel Standard Borosilicate (Schott Duran) Glass Tubing

OD(mm)

ID(mm)

FILFire-

PolishedLength Quantity Item Price

1.0 0.75 4 3 in. (76 mm) 500 TW100F-3 US$511.0 0.75 3 in. (76 mm) 500 TW100-3 US$471.2 0.90 4 4 3 in. (76 mm) 350 TW120F-3 US$561.2 0.90 3 in. (76 mm) 350 TW120-3 US$471.5 1.12 4 3 in. (76 mm) 225 TW150F-3 US$51

1.5 1.12 4 3 in. (76 mm) 300 TW150-3 US$61

1.0 0.75 4 4 in. (100 mm) 500 TW100F-4 US$51

1.0 0.75 4 4 in. (100 mm) 500 TW100-4 US$53

1.2 0.90 4 4 in. (100 mm) 350 TW120F-4 US$511.2 0.90 4 in. (100 mm) 350 TW120-4 US$471.5 1.12 4 4 in. (100 mm) 300 TW150F-4 US$51

1.5 1.12 4 4 in. (100 mm) 225 TW150-4 US$53

1.0 0.75 4 6 in. (152 mm) 500 TW100F-6 US$76

1.0 0.75 4 6 in. (152 mm) 500 TW100-6 US$76

1.2 0.90 4 4 6 in. (152 mm) 350 TW120F-6 US$821.2 0.90 6 in. (152 mm) 350 TW120-6 US$701.5 1.12 4 6 in. (152 mm) 225 TW150F-6 US$76

1.5 1.12 4 6 in. (152 mm) 225 TW150-6 US$76



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To select the best patch clamp glass for your exper-iments, we have evaluated available glass types in terms of the four properties most crucial to successful patch clamp studies:

The softening temperature determines how easily each glass type can be pulled to the desired shape and the extent to which it can be heat polished. Glass with a high softening temperature is difficult to pull and causes unnecessary wear on the heating element of the puller. This makes it very hard to make electrodes that are reproducible and of consistent quality. Patch clamp glass with a low softening temperature is preferred; however, higher softening temperature glass is stronger.

Electrical properties determine how much noise the glass is likely to produce in recording situa-tions. The lower the product of dielectric constant times the loss factor, the smaller the equivalent noise current the glass will produce (Rae and Levis, Methods in Enzymology, 207, p67, 1992). Patch clamp glass with good electrical properties is critical especially in single-channel recording.

Sealability: It is not clear what factors determine the sealing ability of the patch to the glass. Almost any glass can form a gigohm seal under the right conditions. Different glass types vary, however, in how easily they form a seal. It is important to select a patch clamp glass that seals easily. Good fire polish is critical for seal (see DMF1000).

Leachable components: Substances leached from glass can alter channel behavior. Since different channels are sensitive to different glass compo-nents, it is best to record one type of channel with several different kinds of pipette glass to eliminate any artifact due to the glass.

Patch Clamp Capillary GlassWPI offers capillary tubing made from two glass types widely used in constructing patch clamp electrodes. The significant characteristics of each are as follows:

PG52151-4, PG52152-4 and PG52165-4 are prepared from Schott #8250 glass (equivalent to Corning #7052), one of the most widely used patch clamping glasses. This is a specially formulated borosilicate glass with a softening temperature that is 110°C lower than regular borosilicate glass (Corning 7740, or Pyrex). It has excellent sealing properties for most cells. Electrical properties are also very good.

PG10150-4 and PG10165-4 are composed of Corning #0010 glass, a high lead content (22% PbO) glass. Its thermal and electrical performance is between the Schott #8250 and Corning #8161 glasses described above. It is much more econom-ical than Corning #8161 glass. It has been found that this glass causes much less alteration in channel behavior than Corning #8161 and Schott #8250 glass (Furman and Tanaka, Biophys. J. 53, p287, 1988).

Patch clamp capillaries do not have microfilaments.

Glass Handling ForcepsEver had difficulty picking up a glass capillary? Special tips on these forceps solve the problem, holding glass firmly without risk of breakage. They also keep the glass clean and avoid contamination from skin oils.



PATCH CLAMP CAPILLARY GLASS

Catalog# Glass Type OD/ID (mm)Dielectric Constant

Softening Point °C

Quantity Price

PG52151-4 #8250 1.5/1.0 4.9 720° 100 US$54PG52152-4 #8250 1.5/0.9 4.9 720° 100 US$54PG52165-4 #8250 1.65/1.1 4.9 720° 100 US$54PG10150-4 #0010 1.5/0.75 6.7 625° 100 US$54PG10165-4 #0010 1.65/1.1 6.7 625° 100 US$54

77020 Glass Handling Forceps US$45

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Multi-barrel configurations are designed especially for microiontophoresis. Because the capillaries are fused together during manufacture, you will not need to twist them while pulling to seal the tips together. An inner filament in each barrel makes filling easy and fast.

Also see PolyFil for a novel way to connect multi-barrel pipettes

Two-Barrel Three-Barrel Five-Barrel Seven-Barrel

Special Configuration Borosilicate CapillariesSeptum Theta offers superior cell impalement. The natural bevel resulting from the prominent spear-like projection of the septum gives microelectrodes a sharp, spear-point tip. This style has low resistance for use as a single microelec-trode, and it can be used to make superior double-tipped microelectrodes with low trans-tip coupling. The natural bevel of Septum Theta also significantly increases the effective tip cross-section. As supplied, the width of the septum is approxi-mately 0.2 mm; wall thickness is approximately 0.2 mm.

Piggyback glass consists of a pair of boro-silicate capillaries fused together during manufacture. One barrel is larger than the other, and both have inner filaments for quick filling. Piggyback glass makes it simple to fabricate two-barrel electrodes with a significant tip diameter differential.

Septum Theta Piggyback

Borosilicate glass rod1.0 mm diameter — for making tools, probes, tips

Multi-Barrel Glass Capillaries



Borosilicate Glass Rod

Description OD (mm) Length Quantity Item Price

Glass Rod 1.0 4 in. (102 mm) 500 GR100-4 US$53Glass Rod 1.0 6 in. (152 mm) 500 GR100-6 US$59

Special Configuration Borosilicate Glass Tubing

Description OD/ID (mm) Length Quantity Item Price

Septum Theta 1.5/1.02 6 in. (152 mm) 100 TST150-6 US$86

Piggyback1.51/0.840.75/0.35

4 in. (102 mm) 50 PB150F-4 US$62

Piggyback1.51/0.840.75/0.35

6 in. (152 mm) 50 PB150F-6 US$92

Multi-Barrel Borosilicate Glass Tubing with Filaments

Length Description OD/ID (mm) Filament Quantity Item Price

4 in. (102 mm) Two-Barrel 1.5/0.84 4 100 2B150F-4 US$62

4 in. (102 mm) Three-Barrel 1.2/0.68 4 100 3B120F-4 US$62

4 in. (102 mm) Five-Barrel 1.2/0.68 4 65 5B120F-4 US$62

4 in. (102 mm) Seven-Barrel 1.2/0.58 4 60 7B100F-4 US$86


6 in. (152 mm) Two-Barrel 1.5/0.84 4 100 2B150F-6 US$74

6 in. (152 mm) Three-Barrel 1.2/0.68 4 100 3B120F-6 US$74

6 in. (152 mm) Five-Barrel 1.2/0.68 4 65 5B120F-6 US$74




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Five-and Seven-Barrel Micropipette BlanksHigh quality multi-barrel micropipette blanks for pressure injection

PI-305A Pressure Ejection Kit contains 20 plastic fittings, an insertion tool, five 5-ft lengths of colored tubing, a five-valve manifold coupling, six male luer adapters and one male/female luered line. These Pressure Ejection fittings connect the pressure ejection tubing to the P-5 and P-7 pipette barrels quickly and securely.

P-5 and P-7 pipette blanks exhibit levels of quality and repro-ducibility impossible to match in handmade pipettes. They are compatible with most vertical pipette pullers that accommodate 3.00 mm diameter glass. P-5 and P-7 blanks have a solid ball pulling tip and are fabricated from 3.00 mm Kwik-Fil tubing. All barrels except the large central one have filaments.

See PMP-107 Programmable Multipipette Puller, Page 33.

The P-5 and P-7 micropipettes are designed for precise local application of chemicals in extracellular electrophysiological studies. Drug application may be by one of two methods:

1. microiontophoretic ejection of charged substances, or 2. pressure ejection.

Micropipette Storage Jar

Stores up to 30 micropipettes, filled or unfilled, up to three inches in length. A gen-tle sliding action inserts or removes pipettes

without damage to the delicate tips.

82 mm (31 ⁄4")



E210 Storage Jar for 1.0 mm OD Micropipettes US$45E212 Storage Jar for 1.2 mm OD Micropipettes US$45E215 Storage Jar for 1.5 mm OD Micropipettes US$45E220 Storage Jar for 2.0 mm OD Micropipettes US$45

REPLACEMEnT PARTS1965 Foam Ring for 0.75 - 1.0 mm glass US$171966 Foam Ring for 1.2 - 1.5 mm glass US$171967 Foam Ring for 2.0 mm glass US$17

P-5-50 5-Barrel Micropipette Blank (pkg of 50) US$661P-5-10 5-Barrel Micropipette Blank (pkg of 10) US$173P-7-10 7-Barrel Micropipette Blank (pkg of 10) US$224PI-305A Pressure Ejection Kit (-1 kit-) US$10915978 P-5 & P-7 Adapter for use with PUL-100 puller US$541MBMPH5-7 Holder for P-5 & P-7 Pipettes US$173

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TM

Eliminate the cost and trouble of making your own micropipettes — WPI can quickly supply your need for consistently sized pre-pulled glass micropipettes for injection of dyes or proteins into cells, oocytes and for many other biomedical laboratory applications. Tip diameters (ID) range from 0.1 to 10 micrometers.

● Schott Duran borosilicate glass

● 0.5 micrometer and smaller ID micropipettes include an internal glass fiber for easy filling

● Tip inner diameter tolerance ±20%

● Short taper yields high strength

● Nominal length ≈ 50 mm

● OD:ID = 1.33:1

● Standard capillary outer diameters are 1.0 mm (thin-wall) or 1.14 mm

● Every pipette individually tested and inspected

● Vacuum packed

Borosilicate glass micropipettesPlain Shank or

Luer Fittings

Silanized Tips (Luer Shank)Silanization waterproofs the glass to retard water when inserting into cell. This will not let the out-side fluid run down the pipette and get inside so easily.

* 10 µ (ID), 1.14 mm capillary pipettes are for use in WPI’s Nanoliter 2000.

This micro cannula is ideal for placement in the carotid or femoral artery of mice, rats, and other small animal blood vessels. It can be used with a pressure transducer (WPI’S BLPR) for blood pressure measurement, or in conjunction with a micro-syringe injection system (like WPI’s UMPII or MMP pumps). The incorporated standard female luer fitting makes connecting to existing experimental plumbing quick and easy. The cannula is provided with a contoured-tip stainless steel stylet (trocar) to facilitate placement using established techniques. A movable “shoulder” ring provides a tie-in point to prevent accidental removal. The cannula may be left in place for 2 hours or more, and with proper care and cleaning, may be re-used multiple times. Instructions for use included.

Micro Cannula● 0.4mm O.D., 0.2mm I.D. tubing● Autoclavable● Biocompatible Perfluorocarbon tubing material



µTIP SAMPLER ASSORTMENTS

TIPMIX01-05 Two each, 0.1, 0.2, 0.3, 0.4, 0.5 µm ID, plain shank US$91TIPMIX05-10 Two each, 0.5, 1, 2, 5, 10 µm ID, plain shank US$102TIPMIX01-05-L Two each, 0.1, 0.2, 0.3, 0.4, 0.5 µm ID, Luer US$102TIPMIX05-10-L Two each, 0.5, 1, 2, 5, 10 µm ID, Luer US$114

KZ1101 Micro Cannula, 3"

ShankTip I .D .

ShankLength

Glass O .D . FilamentFire

PolishedCatalog #

Price(pack of 10)

PLAIN 0.1 µm — 1.0 mm Thin-Wall Yes No TIP01TW1F US$610.2 µm — 1.0 mm Thin-Wall Yes No TIP02TW1F US$680.3 µm — 1.0 mm Thin-Wall Yes No TIP03TW1F US$680.4 µm — 1.0 mm Thin-Wall Yes No TIP04TW1F US$680.5 µm — 1.0 mm Thin-Wall Yes No TIP05TW1F US$681 µm — 1.0 mm Thin-Wall No Yes TIP1TW1 US$792 µm — 1.0 mm Thin-Wall No Yes TIP2TW1 US$795 µm — 1.0 mm Thin-Wall No Yes TIP5TW1 US$7910 µm — 1.0 mm Thin-Wall No Yes TIP10TW1 US$7910 µm — 1.14 mm A203XV glass * No Yes TIP10XV119 US$9130 µm — 1.0 mm Thin-Wall No Yes TIP30TW1 US$114

LUER 0.1 µm — 1.0 mm Thin-Wall Yes — TIP01TW1F-L US$790.2 µm — 1.0 mm Thin-Wall Yes — TIP02TW1F-L US$790.3 µm — 1.0 mm Thin-Wall Yes — TIP03TW1F-L US$790.4 µm — 1.0 mm Thin-Wall Yes — TIP04TW1F-L US$790.5 µm — 1.0 mm Thin-Wall Yes — TIP05TW1F-L US$791 µm — 1.0 mm Thin-Wall No — TIP1TW1-L US$912 µm — 1.0 mm Thin-Wall No — TIP2TW1-L US$915 µm — 1.0 mm Thin-Wall No — TIP5TW1-L US$9110 µm — 1.0 mm Thin-Wall No — TIP10TW1-L US$9130 µm — 1.0 mm Thin-Wall No — TIP30TW1-L US$114

LUER/SILANIZED 5 µm 1 inch 1.0 mm Thin-Wall No — TIP5TW1LS01 US$1025 µm 2 inch 1.0 mm Thin-Wall No — TIP5TW1LS02 US$10210 µm 1 inch 1.0 mm Thin-Wall No — TIP10TW1LS01 US$10210 µm 2 inch 1.0 mm Thin-Wall No — TIP10TW1LS02 US$10230 µm 1 inch 1.0 mm Thin-Wall No — TIP30TW1LS01 US$12530 µm 2 inch 1.0 mm Thin-Wall No — TIP30TW1LS02 US$125



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Pipette pulled from multi-barrel glass capillary

A drop of hot glue provides a secure, leak-free seal .

PolyFil™

Multi-barrel micropipette coupling kit

MicroFil™ inserted into each barrel

PolyFil allows easy and secure coupling of a multi-barrel micropipette to a pressure source. Coupling is achieved by bonding temperature-resistant and flexible MicroFil to the capillary tube with hot melt adhesive. The luer end of each MicroFil is connected to PVC tubing (200 PSI rated). Kits also include a five-port manifold that allows use of a single PV800 Series PicoPump to drive up to six micropipette barrels independently by switch-ing on only the barrels to be injected. All connections are locking luers — pressure safe and convenient.

Kit includes: 1 pipette holder/handle, plastic; 7 pieces MF28G MicroFil; 7-pieces tubing with male luer lock fittings; 1 flow-thru manifold with five luer lock ports; 1 hot melt glue gun(110V only); 3 glue sticks.

Nonmetallicsyringe needle for

filling micropipettes

WPI’s MicroFil™ fills micropipettes easily and reli-ably. Its long and fine tip allows you to start the filling very close to the pipette tip, eliminating both air bubble formation and clogging due to the washing down of dust particles. The transpar-ent amber MicroFil needle is constructed from a combination of plastic and fused silica — no metal components are used. The MicroFil needle can be stored for days with the filling solution inside without clogging.

U.S

. PAT

ENT

5,2

94

,32

5The MicroFil’s tip elasticity is sturdy and very flexible though not unbreakable. Since it is more flexible than stainless steel needles, moderate bending will not block or damage the MicroFil needle. The combination of plastic and fused silica in the MicroFil tip is sturdier than plastic tips, allowing easy and repeated insertions into micropipettes. MicroFil’s luer fitting allows easy coupling to syringes and syringe filters.

MicroFil™



1-5 pkgs 6-10 pkgs

MF34G-5 MicroFil, 34 ga., 67 mm long (pkg of 5) US$49 US$39MF28G-5 MicroFil, 28 ga., 97 mm long (pkg of 5) US$49 US$39MF28G67-5 MicroFil, 28 ga., 67 mm long (pkg of 5) US$49 US$ 39

CUSTOM MICROFILAll MicroFil products, including custom orders, can be shipped immediately. Custom orders for special needs can be made using nine sizes of MicroFil tubing in lengths up to 50 cm — except for CMF90UxxL which has a maximum length of 10 cm because of its high resistance to flow. Quantity discounts available. Specify length when ordering by inserting the length (in centimeter increments) into the catalog number in place of the XX’s.

CMF20GxxL MicroFil, 20 Gauge, 700 µm ID, 850 µm OD (pkg of 4) US$79CMF22GxxL MicroFil, 22 Gauge, 530 µm ID, 700 µm OD (pkg of 4) US$79CMF23GxxL MicroFil, 23 Gauge, 530 µm ID, 665 µm OD (pkg of 4) US$79CMF26GxxL MicroFil, 26 Gauge, 320 µm ID, 430 µm OD (pkg of 4) US$79CMF28GxxL MicroFil, 28 Gauge, 250 µm ID, 350 µm OD (pkg of 4) US$79CMF31GxxL MicroFil, 31 Gauge, 100 µm ID, 238 µm OD (pkg of 4) US$79CMF34GxxL MicroFil, 34 Gauge, 100 µm ID, 164 µm OD (pkg of 4) US$79CMF35GxxL MicroFil, 35 Gauge, 75 µm ID, 144 µm OD (pkg of 4) US$79CMF90UxxL MicroFil, approx. 36 Gauge, 20 µm ID, 90 µm OD (pkg of 4) US$79

5440 PolyFil Multi-Barrel Micropipette Coupling Kit US$20113316 Mini Glue Gun and (3) glue sticks US$23

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186

Micropipette Holders & Half-Cells

35.7 mm

2 mm Pinto Ag Wire

9.5 mm DIA.

2 mm Pinto Ag Wire

34.9 mm

O-Ring

9.5 mm DIA.

25.4 mm

25.4 mm9.5 mm DIA.

O-Ring

2 mm Jack to Ag Wire

126 mm

30 mm

Female “Luer” Port

9.5 mm DIA.

for 3 mm OD glass

Optional

Handle 20.7 mm

2 mm Pinto Ag/AgCl Pellet

45°

6.3 mm DIA.

2.500

∅0.250

∅1.5 mm

∅1.2 mm

2.500

∅0.250∅1.5 mm

∅1.2 mm

MALE “LUER” PORT

20.7 mm

4.7 mm DIA.


Gasket

EHB1 EHBF

MEH145

20.7 mm 6.3 mm DIA.

2 mm Jackto Ag/AgCl Pellet

45°

MBMPH5-7

Holder for P-5 and P-7 multibarrel glass (see page 81)

WPI’s microelectrode holder-half-cells couple fluid-filled glass micropipettes to high input impedance amplifiers. A Ag/AgCl pellet (or a silver wire) molded into the holder body provides stable potential. Electrical connection is made via male 2 mm pins or female 2 mm sockets. The pipette may be mounted axially or at right angles to the holder. Pipettes are held with screw-caps or rubber gaskets (without caps). Filling WPI microelectrode holders with electrolytes containing chloride results in stable electrode potential. Suitable electrolytes include KCl, NaCl and CaCl2. Holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. (Call WPI regarding custom designs for other glass diameters.) The holder style you select will depend on your experimental application, space, and instrumentation.

Hints for selecting and ordering micropipette holders

1 . Determine the required electrical connection on the holder: for example, if you wish to connect the holder to a 2 mm pin you should select a holder equipped with a 2 mm jack. Most WPI probes require a holder equipped with a 2 mm jack.

2 . Decide on the required alignment of the electrical connection: either in-line with the glass pipette, or at a right angle to it. Space considerations in your experimental setup and requirements imposed by other pieces of equipment typically determine which alignment is appropriate.

3 . Determine if you want to hold the glass pipette by a rubber gasket (e.g., MEH1S) or a screw-cap (e.g., MEH3S). Rubber gaskets offer easier insertion and removal of glass pipettes whereas screw-caps provide more secure mounts for micropipetters.

4 . Choose a holder with either a silver wire or a silver/silver chloride pellet for the metal/liquid coupling. Silver/silver chloride pellets provide a more stable low-noise baseline which is important for low-noise DC recording. Pellets require the glass pipette and holder to be free of air bubbles to achieve a good connection. Silver wire holders are durable and are easier to use when the holder is equipped with a pressure port because the fluid in the pipette does not have to be filled to the top of the pipette to achieve a good electrical connection

5 . Choose a holder equipped with a pressure port only when you want to pressure inject liquid from the pipette. Two types of ports are available: 2.0 mm O.D. and standard “syringe-style” luer. The luer port is often recommended because it makes assembly and disassembly much easier. Quick-connect luer fittings for four common sizes of tubing (1/16", 3/32", 1/8", 5/32" I.D.) are included with each luer-equipped holder.

6 . Some non-WPI preamplifiers or headstages cannot be mounted on micromanipulators. In such cases, a holder equipped with a rod (e.g., MEH8) permits the holder to be conveniently mounted on a micromanipulator.

7 . Finally, remember to specify the O.D. of the glass you will be using when you place your order.

MEH6RF/SF is designed primarily for use with the Model 900A Micropressure System; EHB1 for use in electrode beveling; and MEH3SW for microtitration of chloride with a silver wire as the electrode and a solution of silver nitrate filling the holder. MPH models do not contain Ag/AgCl half-cells and are used for pressure injection of substances through microelectrodes. PicoNozzle, used for pressure injection with PV800 Series PicoPumps, includes an MPH6S holder — which may also be used to couple a micropipette to a syringe. APEH models are also designed for use in pressurized injection procedures.

FOIMPH FOIMPH-LF

9.5 mm DIA.


35.7 mm 35.7 mm

2 mm Pinto Ag Wire

9.5 mm DIA.APEH1 APEH2

MEH1RMEH1F45





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MICROELECTRODE HOLDERS Electric Connection Pressure Screw Designed for Angle Connector Half-Cell Port Cap WPI Products Price

APEH1 Right Male Pellet No Port 2 Caps US$68APEH2 Right Male Wire No Port 2 Caps US$68EHB1 Straight Male Wire No Port N/A MBS, 48000 US$41EHBF Straight Female Wire No Port N/A MBS, 48000 US$47FOIMPH Straight Fiber Optic None No Port w/Cap MBS, 48000 US$56FOIMPH-LF Straight Fiber Optic None Male Luer w/Cap MBS, 48000 US$69MBMPH5-7 ---- None None Female Luer w/Cap US$114MEH145 45° Male Pellet No Port No Cap US$53MEH1F45 45° Female Pellet No Port No Cap 705, 773, 767, 721, FD223 US$53MEH1R Right Male Pellet No Port No Cap US$41MEH1RF Right Female Pellet No Port No Cap 705, 773, 767, 721, FD223 US$41MEH1S Straight Male Pellet No Port No Cap US$41MEH1SF Straight Female Pellet No Port No Cap 705, 773, 767, 721, FD223 US$41MEH2R Right Male Pellet Male Luer w/Cap US$41MEH2RF Right Female Pellet Male Luer w/Cap 705, 773, 767, 721, FD223 US$41MEH2RFW Right Female Wire Male Luer w/Cap 705, 773, 767, 721, FD223 US$47MEH2RW Right Male Wire Male Luer w/Cap US$47MEH2S Straight Male Pellet Male Luer w/Cap US$41MEH2SF Straight Female Pellet Male Luer w/Cap 705, 773, 767, 721, FD223 US$41MEH2SFW Straight Female Wire Male Luer w/Cap 705, 773, 767, 721, FD223 US$47MEH2SW Straight Male Wire Male Luer w/Cap US$47MEH345 45° Male Pellet No Port w/Cap US$53MEH3F45 45° Female Pellet No Port w/Cap 705, 773, 767, 721, FD223 US$53MEH3FW45 45° Female Wire Port w/Cap US$53MEH3R Right Male Pellet No Port w/Cap US$41MEH3RF Right Female Pellet No Port w/Cap 705, 773, 767, 721, FD223 US$41MEH3RFW Right Female Wire No Port w/Cap 705, 773, 767, 721, FD223 US$41MEH3RW Right Male Wire No Port w/Cap US$41MEH3S Straight Male Pellet No Port w/Cap US$41MEH3SB Straight Banana Pellet No Port w/Cap ISO-80, ISO-DAM8A US$47MEH3SBW Straight Banana Wire No Port w/Cap ISO-80, ISO-DAM8A US$47MEH3SF Straight Female Pellet No Port w/Cap 705, 773, 767, 721, FD223 US$39MEH3SFW Straight Female Wire No Port w/Cap 705, 773, 767, 721, FD223 US$39MEH3SW Straight Male Wire No Port w/Cap US$39MEH3W45 45° Male Wire No Port w/Cap 705, 773, 767, 721, FD223 US$53MEH6RF Right Female Pellet 2.0-mm Port w/Cap 705, 773, 767, 721, FD223 US$47MEH6RFW Right Female Wire 2.0-mm Port w/Cap 705, 773, 767, 721, FD223 US$47MEH6SF Straight Female Pellet 2.0-mm Port w/Cap 705, 773, 767, 721, FD223 US$47MEH6SFW Straight Female Wire 2.0-mm Port w/Cap 705, 773, 767, 721, FD223 US$47MEH7 Right Male Pellet 2.0-mm Port w/Cap US$68MEH7W Right Male Wire 2.0-mm Port w/Cap US$68MEH8 Right Male Pellet No Port w/Cap US$68MEH900R Right Male Pellet 2.0-mm Port w/Cap 900A US$47MEH900S Straight Male Pellet 2.0-mm Port w/Cap 900A US$47MPH1 — None None Female Luer w/Cap US$55MPH3 — None None Male Luer w/Cap US$55MPH4 — None None 2.0-mm Port w/Cap US$55MPH6P Right Male Pellet Female Luer w/Cap Piconozzle Kit (5430-XX) US$41MPH6R Right Male Wire Female Luer w/Cap Piconozzle Kit (5430-XX) US$41MPH6S — None None Female Luer w/Cap Piconozzle Kit (5430-XX) US$41

SPECIFY O.D. OF GLASS (‑1.0, 1.2, 1.5 OR 2.0 mm‑) WHEN ORDERING.

Handles and Accessories (not included)Handle #2505 is for use with WPI manipulators. The smaller diameter handle #5444 is required for use with Narishige and Zeiss manipulators.

2505 1/4-in (6.3 mm) diameter handle US$165444 3/16-in (4.8 mm) diameter handle US$32GO1-100 Replacement gasket 1.0 mm, Package of 100 US$33GO2-100 Replacement gasket 1.2 mm, Package of 100 US$33GO3-100 Replacement gasket 1.5 mm, Package of 100 US$33GO4-100 Replacement gasket 2.0 mm, Package of 100 US$331571 Clear Silicone Rubber Sealant (-4.7 oz-) US$29

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27.2 mm

6.3 mm DIA.

2 mm Jackto Ag Wire

45°

27.2 mm


6.3 mm DIA.

188

6.3 mm DIA.

Male“Luer”

Port

2 mm Pinto Ag Wire

27.2 mm

MEH2RW

6.3 mm DIA.

36.7 mm

Male“Luer”Port

2 mm Pin to Ag Wire

27.2 mm

MEH2SW

MEH3R

27.2 mm


Male“Luer”Port

36.7 mm

6.3 mm DIA.

MEH2S

6.3 mm DIA.

Male“Luer”Port


29 mm

MEH2SF

27.2 mm


6.3 mm DIA. MEH3RF

29 mm


Male “Luer” Port

6.3 mm DIA.

MEH2SFW

27.2 mm

2 mm Jackto Ag WireMale

“Luer”Port

6.3 mm DIA.

MEH2RFW

27.2 mm

2 mm Pinto Ag/AgCl PelletMale

“Luer”Port

6.3 mm DIA.MEH2R

6.3 mm DIA.

Male “Luer” port


27.2 mm

MEH2RF

23.9 mm


4.7 mm DIA. MEH1SF

MEH3FW45

27.2 mm6.3 mm DIA.


45°

MEH3F45

27.2 mm 6.3 mm DIA.


45°

MEH345

6.3 mm DIA.

2 mm Pinto Ag Wire

27.2 mm

MEH3RW6.3 mm DIA.

2 mm Jackto Ag Wire

27.2 mm

MEH3RFW

6.3 mm DIA.

35.2 mm

Mini-Banana Plugto Ag/AgCl Pellet

27.2 mm

MEH3SB

27.2 mm


36.7 mm 6.3 mm DIA.

MEH3S


4.7 mm DIA.

23.9 mm

33.5 mm

Gasket

MEH1S


6.3 mm DIA.

20.7 mm

Gasket

MEH1RF





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42 mm

6.3 mm DIA.

FemaleLocking “Luer”Port

6.3 mm DIA.

2 mm Jackto Ag Wire

29 mm

123.7 mm

27.2 mm


6.3 mm DIA.

2.0 mm O.D.Port

Optional

Handle

29 mm


6.3 mm DIA.

MEH3SF

27.2 mm

Mini-Banana Plugto Ag Wire

35.2 mm

6.3 mm DIA.

MEH3SBW

123.7 mm

27.2 mm

MaleLuer

Port

Optional

Handle

6.3 mm DIA.

126 mm

30 mm

FemaleLuerPort

9.5 mm DIA.

Optional

Handle

MPH1 MPH3

123.7 mm OptionalHandle

4.7mm DIA.

6.3mm DIA.27.2 mm

6.3 mm DIA.

2.0 mm O.D.Port

MPH46.3 mm DIA.

2 mm PINto Ag WIRE

42 mm


MPH6R

6.3 mm DIA.


27.2 mm

123.7 mm Optional

Handle

2.0 mm O.D.Port

6.3 mm DIA.

2 mm Pinto Ag Wire

27.2 mm

123.7 mm Optional

Handle

MEH7W MEH8

29 mm

2.0 mm O.D. Port


6.3 mm DIA.33.6 mm

27.2 mm


2.0 mm O.D. Port

36.7 mm

6.3 mm DIA.

29 mm


6.3 mm DIA.

2.0 mm O.D. Port

2.0 mm O.D. Port

6.3 mm DIA.


29 mm

6.3 mm DIA.

36.7 mm

2 mm Pin to Ag Wire

27.2 mm

27.2 mm

6.3 mm DIA.

2 mm Pinto Ag Wire

45°

29 mm

2.0 mm O.D. Port


6.3 mm DIA.

33.6 mm33.6 mm

6.3 mm DIA.

2 mm Jackto Ag Wire

2.0 mm O.D. Port

29 mm

6.3 mm DIA.

2 mm PINto Ag WIRE

42 mm


189

MEH7

MPH6S

MEH3SW

MEH6RFW

MEH900S

MEH3W45

MEH900R

MEH6SFW

MEH6SF

MEH3SFW

MEH6RF

MPH6P



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WPI's new BetterSkin™electrodes are high quality Ag/AgCl reusable surface-mounted electrodes designed to be used for the acqui-sition of all biopotentials. Each electrode is fabricated using an optimum quality sintered Ag/AgCl pellet encased within a strong epoxy housing designed to have an extended lifetime. The result is a superior electrode that provides accurate and clear transmission of even the smallest surface biopotentials. In comparison to standard “stamped” metal electrodes and Ag/AgCl disposable electrodes, WPI's BetterSkin electrodes provide increased stability, lower noise and lower offset voltage during surface biopotential recording. These features are par-ticularly important during measurement of very small potential signals, such as EMG or EEG recording.

Four BetterSkin electrode sizes are currently offered: 7-mm, 13-mm, 16-mm and 19-mm (OD). The 7-mm electrodes are designed for use where close spacing between biopotential recording sites is required. The medium sized

electrodes (13-16 mm OD) are general purpose electrodes and appropriate for most applica-tions. The 19-mm large size electrodes are designed for optimal surface skin contact for use during long-term biopotential recording.

Three different connector and cable combi-nations are offered: a 2-mm pin with 1 meter unshielded cable; a 2-mm pin with 1 meter

DIN socket

now available!DIN socket

now available!

BetterSkin™ Electrodes● WPI’s unique Ag/AgCl pellet manufacturing process gives

increased mechanical strength for extra durability

● Highly porous surface for low impendence, high current passing capability and stable, low noise measurements

● High flex cable comes in shielded and unshielded form (Carbon fiber cable for NMR environment is available upon request)

● Strong epoxy house increases durability and makes cleaning easy

● Ultra-high purity material for low offset and drift

shielded cable (shielding is connected to an additional 2 mm grounding pin); and 1.5 mm DIN safety socket with 1 meter unshielded cable. All electrodes require adhesive disks (e.g., WPI's ADD200 series) and recording gel (GEL100).

Bulk discount orders are possible and trade-inquiries are also welcome.



Skin Electrodes with 2 mm Gel Capacity and 1 m Lead Discs

OD SensorDiam.

HousingHeight

PinDiam. Shield Ground

Pin Diam.DIN

SocketElectrode

Price

AdhesiveDisc

(pkg of 100)

DiscPrice

EL204 7 mm 4 mm 5 mm 2 mm — — — ADD204EL204S 7 mm 4 mm 5 mm 2 mm Yes 2 mm — ADD204EL204D 7 mm 4 mm 5 mm — — — 1.5 mm ADD204EL208 13 mm 8 mm 6 mm 2 mm — — — ADD208

EL208WS 16 mm 8 mm 6 mm 2 mm Yes 2 mm — ADD208EL208D 13 mm 8 mm 6 mm — — — 1.5 mm ADD208

EL208WD 16 mm 8 mm 6 mm — — — 1.5 mm ADD208EL212 19 mm 12 mm 7 mm 2 mm — — — ADD212

EL212S 19 mm 12 mm 7 mm 2 mm Yes 2 mm — ADD212

Conductive Electrode GelA very high quality non-iritating conductive multipurpose electrode gel for use with skin electrodes that are not pre-gelled. Ideal for use in EEG, ECG, and EMG applications.

Tube contains 8 oz. (250 g).

Shipping weight: 1 lb. (0.5 kg).

GEL100 Conductive Electrode Gel

Disposable Ag/AgCl Snap ElectrodesDisposable Ag-AgCl snap adhesive electrodes. Pre-gelled for simple peel and stick application. An ideal resting ECG electrode. Size: 0.875 x 1.75 inches. (Electrode lead available separately.)

Ag/AgCl PROBES & Ag/AgCl SNAP ELECTRODESEL203 Disposable Snap Electrodes (package of 30) 500131 Snap Cable, 1m, 2mm pin (package of 5) 500330 Snap Cable, 7-lead, DIN (1.5 mm pin)



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191

13 mm4 mm DIA.


15.9 mm3.9 mm DIA.

2 mm DIA. Gold Plated Pinto Ag/AgCl Pellet

RC1 / RC1T

RC2FRC2

EP22 mm dia.4 mm long

Ag Wire 5 cm long

EP88 mm dia.1 mm thick

Ag wire 12 mm long Ag wire 12 mm long



Ag wire 12 mm long

EP11 mm dia.

3 mm long

Ag Wire 70 mm long

EP080.8 mm dia.2 cm long

Ag Wire 60 mm long

EP050.5 mm dia.2 cm long

Ag Wire 60 mm long

RCI has an unterminated lead. RC1T is terminated

with a 2 mm pin.

25.4 mm

8.5 mm

Insulator

4 mm DIA. 2 mm DIA.Ag/AgCl Pellet

#24 AWGinsulated wire,4 ft

50 mm

25 mm

4.5 mm diam.

3.5 mm diam. Ag/AgCl pellet

epoxy body

75 mm

25 mm

4 mm diam.


glass body

53 mm

1.5 m insulated lead

4 mm diam.3 mm


glass body

50 mm

25 mm

1.5 mm diam.

1 mm diam. Ag/AgCl pellet

glass body

RC3 RC4

RC5 RC6



Ag/AgCl Half-CellsNew, improved sintered pellets with lower resistance and high strength. Stable and well balanced in the presence of current, these small and inex-pensive half-cells are easy to work with as bath electrodes.

RC1 Reference Cell with 1.5 m leadRC1T Reference Cell, 1.5 m lead, 2 mm pinRC2 Reference Cell with 2.0 mm pinRC2F Reference Cell with female connectorRC3 Reference Cell with epoxy body, 4.5 mm diam x 50 mmRC4 Reference Cell with glass body, 4.0 mm diam x 75 mmRC5 Reference Cell with glass body, 4.0 mm diam x 50 mmRC6 Reference Cell with glass body, 1.5 mm diam x 50 mmEP05 Ag/AgCl Electrode 0.5 mm diam x 2 cmEP08 Ag/AgCl Electrode 0.8 mm diam x 8 mmEP1 Ag/AgCl Electrode 1.0 mm diam x 2.5 mmEP2 Ag/AgCl Electrode 2.0 mm diam x 4 mmEP4 Ag/AgCl Electrode 4.0 mm diam x 1 mmEP8 Ag/AgCl Electrode 8.0 mm diam x 1 mmEP12 Ag/AgCl Electrode 12.0 mm diam x 1 mm3578 Adapter Cable for Ag/AgCl Pellets

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MicromanipulatorsMotorized Micromanipulators

PiezoPatchTM: Piezo-motorized Micromanipulator suitable for patch clamp and IVF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .193

MPM10: Piezo Controller for DC3001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195

MPM20: Piezo Translator for M3301/DC3001 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195

SM325: Compact Programmable High Precision 3-D Micromanipulator System suitable for patch clamp and IVF . . . . . . .196

HS6-3: Solid Programmable Ultra High Precision 3-D Micromanipulator System suitable for patch clamp and IVF . . . . . .197

MM3-MS: Miniature Programmable 1-D Micromanipulator System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .198

DC3314: 3-D Motorized Micromanipulator System with Manual Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .197

Manual Micromanipulators

MM1 & MM1-3: One & three-axis small miniature micromanipulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .198

MM3 & MM3-3: One & three-axis medium miniature micromanipulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .198

KITE: Economic 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200

M3301: Most Popular 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200

HS6: Solid High Precision 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202

MMJR: Additional joystick controller on regular 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202

MD4R: Dual Tool Holder 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .203

M325: Compact Backlash-Free 3-D micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .203

Stands & Accessories

Vibration Control Systems: Vibration-Free Platform and Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207

Digital Micrometer Head with Non-rotating Spindle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194

UMS: Stable Universal Manipulator Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194

TB-1 & M3: Compact Tilting Bases and Weight for Micromanipulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200

TBS: Solid Tilting Base for Micromanipulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .201

Optional Mounting Devices: Clamps and Microscope Stage Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .201

Magnetic Holding Devices: Easy to mount Micromanipulators at desired heights and angles . . . . . . . . . . . . . . . . . . . 204-206

NEW Non-Magnetic Bases, Rods & Clamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176





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PiezoPatchTM Micromanipulator (PPM5000) is a unique piezo-motor driven micromanipulator with ultra high resolution (<1 nm/step), super low drift (<4 nm/h), and long travel range (~10 mm/axis) . It is ideal for patch-clamp, electrophysiology and other high precision applications within the life sciences . PiezoPatch establishes a new standard in micromanipulator design .

At the heart of the PiezoPatch is WPI’s proprietary SonicWaveTM piezo-motor that is controlled at 1 arc-second (1⁄3600 of a degree) per step . This angular resolution translates to a theoretical linear movement of ~0 .4-nm per step, an ultra high resolution that is beyond normal measurement capability using standard methods . Another key advantage of the PiezoPatch motor is the extreme stability . When de-energized, the SonicWaveTM motor is completely locked with absolutely no rotation . This locking mechanism completely eliminates mechanical drift in the micromanipulator . This design is significantly different compared to all other types of electromagnetic motors or piezo-operated micromanipulators, which still require electric power to hold at the stopped position . Electric power can introduce electrical noise, which can interfere with experimental measurements . Furthermore, instabilities in electrical power supply can cause drift of the micromanipulator . Thermal drift is also caused by heat generated in the stopped motor . These problems are completely eliminated by the PiezoPatch SonicWaveTM motor, since it does not require power to maintain its stopped position during the experiment . One additional feature of the SonicWaveTM motor is its extremely high output torque compared to motors of same size . This enables skip free precise movement of the micromanipulator even under heavy load .

PiezoPatch has three distinct movement modes: Step, Continuous, and Penetration . Continuous mode allows the micromanipulator to move quickly (continuously) from the starting position towards the object . Step mode enables ultra fine movements to be controlled by a single step in increments as small as 1 nm per step . Penetration mode is used when the glass micropipette is positioned close to the cell membrane and the x-axis piezo motor is then activated using the joystick button . This causes the micropipette to penetrate the membrane at high speed while minimizing membrane disturbance .

The ergonomic joystick controller can move each axis or all axes in three coordinate directions simultaneously . The speed of the travel is determined by the deflection on the joystick . Since each movement is too

fine to be visually observed, a specially designed acoustic feedback control is included to keep operators aware of the actuation of the manipulator .

To improve the productivity during use, the micropipette can be exchanged rapidly without remounting the entire micromanipulator and spending hours repositioning the capillary . The Rotary Pivot Base allows the micromanipulator to rotate 360° horizontally . This base has a special mounting pattern allowing it to be mounted directly on to a standard Vibration-Free Platform (VFP), Vibration-Free Workstation (VFW), or a Universal Manipulator Stand (501622 or 501623) .

The universal capillary holder and headstage adaptor can be easily rotated and adjusted to the desired height and angle . The headstage adaptor fits most headstages from Axon and HEKA and can be customized to fit other manufacturers headstages (contact WPI) .

PiezoPatch is designed to be fully ambidextrous, therefore there is no need to purchase both left-handed and right-handed versions to fit everyone in the lab .

Headstage adapter

included

PPM5000 SPECIFICATIONSTRAVEL DISTANCE 10 mm each axis

VELOCITY RANGE

“PENETRATION” MODE 10mm/s - 100mm/s

“CONTINUOUS” MODE 0 .5-500 µm/s

“STEP” MODE 0 .005- .5 µm/s

RESOLUTION

“CONTINUOUS” MODE 0 .1 µm

“STEP” MODE 0 .001 µm per step

DEPTH OF PENETRATION

“STEP SIZE” MODE 0 .5-5 µm per step

LONG TERM STABILITY: <4 nm drifting/hour @ 20° C

OPERATING VOLTAGE: 12V DC

SINGLE AXIS CONSUMPTION, MAX . SPEED: <300 mA

TOTAL CONSUMPTION, MAX . SPEED: <900 mA

DIMENSIONS:

ELECROMECHANICAL MODULE: 265(h) x 120(w) x 130(l) mm

10 .4(h) x 4 .7(w)x 5 .1(l) in .

CONTROLLER: 165(h) x 100(w) x 125(l) mm

3 .9(h) x 4 .9(w) x

WEIGHT

ELECTROMECHANICAL MODULE: 2 .2 kg

CONTROLLER: 0 .65 kg

PiezoPatch™ Micromanipulator

Vibration-free Platform (VFP) not included

M3301EH Electrode Holder included

501622 Universal Micromanipulator Stand available separately



PPM5000 PiezoPatch™ Micromanipulator with Joystick Control

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder, straight, 14cm15873 Angled Electrode Holder, 13 cm long501622 Universal Micromanipulator Stand, 30 cm high501623 Universal Micromanipulator Stand, 45 cm highVFP Vibration-Free Platform VFW Vibration-Free Workstation

Rotary 3-axis Controller — Coming Soon

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Two Universal Micromanipulator Stands enable scientists to mount their manual and motorized micromanipulators at variable angles and heights . A solid aluminum platform with a grooved tower allows the user to attach any micromanipulator of any size or shape to the post for infinite flexibility . Once mounted, the micromanipulator can be set at any height along the entire length (30 cm or 45 cm) . The platform base comes configured with industry standard pre-bored holes (1/4-20 x 1” or M6 x 25 mm), allowing direct mounting to any type vibration-free table (for patch clamp recordnings) or optical bench (for laser and optical measurements) .

By using the Optional Rotation Clamp, two or more micromanipulators can be mounted on the stand simultaneously in a space-saving convenient manner . WPI’s Universal Stand not only allows the user 360° flexibility in manipulations but also promotes independent angular transitions using a single feather-light tensioner/adjustment screw . This affordable stand is currently the preferred choice in micromanipulator stands — head and shoulders above set-ups using multiple magnetic-based stands costing hundreds more when combined with antiquated heavy steel base plates .

UMS SPECIFICATIONSDIMENSIONS

Base plate 10 .0 x 12 .5 x 1 .5 cm (LxWxH)

Stand 4 .0 x 4 .0 x 30 cm (LxWxH) (501622)

4 .0 x 4 .0 x 45 cm (LxWxH) (501623)

Mounting holes English 1/4 20 x 1”

Matrix M6 x 25mm grid

SHIPPING WEIGHT 501622 9 lbs (4 kg)

501623 11 lbs (5 kg)

Vibration-free Platform (VFP) not included

Universal Manipulator Stand

Non-rotating Spindle Digital Micrometer HeadBuild your own precision micro-positioning device

The new non-rotating spindle digital micrometer head allows you to create your own micro-positioning instrument . With micron-level accuracy, it gives higher precision than a normal micromanipulator . Since the spindle does not rotate as it advances, instruments can be directly attached without the need for a complicated decoupling device . The digital display eliminates the need to

502102 SPECIFICATIONSTotal Travel Distance 25 mm

Resolution 0 .001 mm

Accuracy ± 0 .003 mm

Spindle Diameter ∅ 8 mm

Mounting ∅ 12 mm x 10 mm

Total Length 166 mm

Measurement Mode Absolute and incremental

Digital Readout mm or inch

Analog Readout mm

Data Output RS232

Environmental Protection IP54

Shipping Weight 0 .51 kg (1 .12 lb)

squint at the notational scale . Readings can be clearly seen in either inches or millimeters . You can read both absolute position and the increment relative to a previously chosen point . In addition, there is a digital output port . With optional cable and software SPG-KIT-MICRONR, you can send measurements to your computer either continuously or every time a button on the micrometer is pushed .



501622 Universal Micromanipulator Stand (30 cm high) 501623 Universal Micromanipular Stand (45 cm high) 501624 Optional Rotation ClampVFP Vibration-Free Platform VFW Vibration-Free Workstation

502102 Non-Rotating Spindle Micrometer Head



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SHigh speed

penetration and precise control

The piezo-ceramic element in WPI’s MPM10 provides high penetration speed over an extremely short distance (0 .5 to 10 .0 µm) . Because the range of travel of a dedicated piezomanipulator is much too limited for it to be useful independently, it must be mounted on a manipulator . The MPM-10 piezo translator combined with a DC3001 motorized micro-manipulator (available separately) provides a single electronically controlled system .

When the piezo element is activated, the MPM-10 axis carrying the micropipette shoots forward at a rate which is set on the control panel, then immediately returns (at a slower speed) to its starting position . As soon as the piezo element begins its reverse travel, the motorized manipulator starts to travel forward . The complimentary opposition of these two travel sequences results in the micropipette tip remaining in its advanced absolute position .

The three axes of the DC3001 are controlled by six buttons . Pressing a button for less than 0 .3 seconds activates one step, the size of which (0 .5 to 10 .0 micron) is set on the Step Size control . Pressing longer activates the continuous mode, at the rate set on the Motor Speed control . Pressing the button for the X axis forward direction activates the piezo mode . Advancement speed of the piezo element can be separately adjusted from 1 to 100 mm/sec .

Precise construction and special vibration stabilizers ensure the MPM-10’s excellent

puncture characteristics . Lateral deviation from the ideal axis of puncture (measured at the tip of the electrode holder) is ± 5% of the step size .

Includes controller, piezo translator, electrode holder, cables and mounting bracket. Shipping weight: 6 lb (2.7 kg).

MPM10

Piezo-Translator

Adapter MPH8 allows use of WPI electrode holders!

DC3001 micromanipulator and M-3 tilting base not included. See page 195.

Piezo TranslatorFor M3301 and DC3001 micromanipulatorsEspecially recommended for use with the M3301 micromanipulator, the MPM20 is a very efficient tool for intracellular injection . High penetration speed and precise axial advance allows injection pipette to be brought to its target position with tremendous accuracy . Lateral escape of the cell is almost eliminated, and even tough membranes can be penetrated . Independently selectable reverse speed setting can be used for fast withdrawal, preventing adhesion of the injected cell to pipette tip . Mounts directly onto DC3001 and M3301 micromanipulators . Use with DC3001 requires MS314 controller (for the micromanipulator) .

The combination of the MPM20, a micromanipulator, and the PV820 PicoPump (see page 234) constitutes an extremely efficient system for intracellular injection; cell penetration, injection and withdrawal are executed automatically with the press of a button .

Shipping weight: 10 lb ( 4 .5 kg ) .

Adapter MPH8 allows use of WPI electrode holders!

MPM20

Micromanipulator not included.



MPM20 Piezo Translator PM7 Replacement Electrode Holder for MPM2014106 Footswitch for MPM10, MPM20


MPM10 Piezo Controller for DC3001 Motorized Micromanipulator PM5 Remote Controller for MPM-10PM6 Replacement Electrode Holder for MPM1014104 Record/Inject Electrode Holder for MPM10, MPM2014106 Footswitch for MPM10, MPM20MPH8 Electrode Holder Adapter for MPM10 & MPM20


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196

WPI introduces a compact high precision motorized microma-nipulator (SM325) . It features low noise, high stability, a user-friendly software interface and economy that are major concerns in IVF and patch clamp research .

The SM325 is driven in all three axes through high resolu-tion stepping motors, which can achieve 40,000 steps per revolu-tion (25 nm/step) with completely vibration-free motion . In a normal lab environment, it can stay local-ized overnight without drifting . The 25mm long range of travel makes it unnecessary to have an additional manual coarse adjust-ment .

Its compact construction makes mounting onto the stage plate of a microscope practical . The x-axis can be tilted by 90° that allows for a better positioning of the injection tool . An additional tilt-ing fixture makes it possible to tilt the tool holder for fast and easy cleaning and exchange of the injection tool .

The MCL3 controller features a dynamic micro-step function that makes very quick positioning possible with maximum accuracy . Motor control is achieved with a linear output amplifier, which also drastically reduces electronic noise . Users can control the micromanipulators by joystick, keyboard, mouse or computer . The user-friendly software program can be enabled to remember up to 999 position coordinates from previous procedures and can robotically repeat this same positioning sequence .

SM325 SPECIFICATIONSCONTROL METHOD Joystick, software, or both

TRAVEL DISTANCE 25 mm each axis

RESOLUTION 25 nm/step or 40,000 steps/rev

MAXIMUM SPEED 4 mm/second

POWER SUPPLY 120/240V, 50/60Hz

DIMENSIONS

SM325-M 5x7x5 .5-in . (13x18x14 cm) (WxLxH)

MCL3 9 .8x9x3 .7-in . (25x23x9 .5 cm) (WxLxH)

SHIPPING WEIGHT

SM325-M 6 lb . (2 .7 kg)

MCL3 11 lb . (5 kg)

Programmable High Precision

Motorized Micromanipulatorsuitable for patch clamp and IVF

SM325-M

MCL3-SM325

Universal Manipulator Stand not included.




SM325 High Resolution 3-D Motorized Micromanipulator (SM325-M) & Controller (MCL3)

SM325-M High Resolution 3-D Motorized MicromanipulatorMCL3-SM325 Controller with Joystick and software for SM325-M

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder, straight, 14cm15873 Angled Electrode Holder, 13 cm long501622 Universal Micromanipulator Stand, 30 cm high501623 Universal Micromanipulator Stand, 45 cm highVFP Vibration-Free PlatformVFW Vibration-Free Workstation



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Programmable Ultra High PrecisionMotorized Micromanipulators

MS314 Controller

Manual coarse controls use cross roller bearing slides . Vernier scales allow . All controls are closely grouped so adjustments can be made in any plane with minimum

effort . The DC3001 features DC motor drives and fine control micrometers in all three axes .

Left- or right-handed versions of the DC3001 are supplied with a standard 12 mm clamp . Standard accessories provided include one microelectrode holder and a securing bolt and wrench .

The sophisticated MS314 Controller allows control of all three axes . Movements may be continuous through the use of cross switches, or the controller can cause the DC3001 to step in defined increments . Steps as small as 0 .5 µ are possible . A popular joystick controller, STM3, allows control of the X, Y and Z axes .

STM3 Controller (available separately) ➡

The HS6-3 is supplied with manual controls and stepper motor drives in all 3 axes . The extremely solid construction eliminates the vibrations and drifts . With the utmost precision and long travel distance in all three directions, HS6-3 is the

ideal tool for patch-clamp or electrophysiological applications . The tilting device is mounted on the base plate serves as coarse height adjustment as well and the tool holder can be swiveled in all directions .

The MCL3 controller features a dynamic micro-step function that makes very quick positioning possible with maximum accuracy and free of vibration . Motor control is achieved with a linear output amplifier, which also drastically reduces electronic noise . Users can control the micromanipulators by joystick, keyboard, mouse or computer . The user-friendly software program can be enabled to remember up to 999 position

coordinates from previous procedure and can robotically repeat this same positioning sequence .

DC3001 SPECIFICATIONS TRAVEL RANGE RESOLUTION

MANUAL: X-axis 37 mm 0 .1 mm

Y-axis 20 mm 0 .1 mm

Z-axis 20 mm 0 .1 mm

TRAVEL RANGE RESOLUTION MAXIMUM SPEED

MOTORIZED: X-axis 10 mm 0 .5 µm 0 .2 mm/sec Y-axis 10 mm 0 .5 µm 0 .2 mm/sec

Z-axis 10 mm 0 .5 µm 0 .2 mm/sec

SHIPPING WEIGHT:

DC3001: 3 lbs (1 .4 kg)

MS314: 1 .8 lbs (0 .9 kg)

STM3: 2 .8 lbs (1 .3 kg)

HS6-3 SPECIFICATIONSCONTROL METHOD: Joystick, software, or both

TRAVEL (X-Y-Z): 25 mm

RESOLUTION: 10 nm/step

MAXIMUM SPEED: 4 .5 mm/sec .

STABILITY: 1 nm/hour at 24°C

POWER SUPPLY: 120/240 V, 50/60 Hz

DIMENSIONS:

HS6-3: 6 .1x9 .7x9 .9-in (15 .5x24 .6x25 cm) (WxLxH)

MCL3: 9 .8x9x3 .7-in (25x23x9 .5 cm) (WxLxH)

WEIGHT:

HS6-3: 13 .2 lb . (6kg)

MCL3: 7 .7 lb . (3 .5kg)

MCL3-HS6

HS6-3

DC3001


15873 Electrode Holder not included

TBS Tilt Base not included



HS6-3 High Resolution Motorized HS6 Micromanipulator and Controller includes HS6-3M and MCL3HS6-3M High Resolution Motorized HS6 Micromanipulator MCL3-HS6 Controller with Joystick and software for HS6-3M

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder, straight, 14cm15873 Angled Electrode Holder, 13 cm long501622 Universal Micromanipulator Stand, 30 cm high501623 Universal Micromanipulator Stand, 45 cm highVFP Vibration-Free Platform VFW Vibration-Free Workstation

DC3314R Manipulator (right-handed) & MS314 Controller DC3314L Manipulator (left-handed) & MS314 Controller

Specify line voltage.Also see magnetic stands.

System components also available separately:DC3001R Motorized Manipulator, right-handed DC3001L Motorized Manipulator, left-handed MS314 Controller for DC3001 STM3 Joystick Controller for DC3001

OPTIONAL ACCESSORIESTBS Tilt Base with Screw Adjustment PM5 Remote controller for MS314 and MPM-10 5464 5-lb Weight for Tilting Base (shipping weight: 7 lb [3 kg]) M2 Additional 12 mm Clamp M-3 80° Tilting Base (Shipping Weight: 2 lb) M4C Microscope Stage Adapter M5 Additional 10 mm Clamp M6 Additional 1⁄2-in . Clamp M3301EH Replacement Electrode Holder (14 cm long) 15873 Angled Electrode Holder (13 cm long) 501607 Cable for MS314 and DC3001

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MM3-MCPROThe MM3-MCPRO Micropositioning Controller was designed for ease of use and effortless integration into any application. The MM3-MCPRO is a low cost, quick setup solution for those applications requiring precise position control of motorized stage (MM3-M). The MM3-MCPRO functions as seamlessly as a single axis, stand-alone system or as a multi-axis networked system, either with or without a PC. The peer-to-peer network architecture makes it possible for every MM3-MCPRO in a multi-axis system to control every axis in the motion system. No software development or complex wiring is required. The controllers themselves may be programmed to store and recall positions and to sequence through stored positions with looping, pausing and user/program interaction. Automation may also be driven serially from a PC.

MM3-M SPeCIfICATIONSLead Screw 80 TPI

GeAR RATIO 64:1

TRAveL 51 mm

CONTROLLeR ReSOLuTION 1 micron

COuNTS/SCRew RevOLuTION 640

HORIzONTAL LOAd CAPACITy 0.5 Kg

veRTICAL LOAd CAPACITy 0.5 Kg

MAxIMuM SPeed 8 mm/sec

SLIdeR BACKLASH 0.05 mm

RuNOuT (MAxIMuM) 0.003 mm/inch

COuNTS PeR MILLIMeTeR 2016

MM3-C clamp

MM3-3micropositioner

MB2 magnetic

base available

separately(see page

204)

MM1-3micropositioner

and MM1-C clamp

MM1-A adapter

SNOITACIfICePSReNOITISOPORCIM-INIM1MM 3-1MM 3MM 3-3MM

SIxA x z-y-x x z-y-x

eNILTHGIARTSyCARuCCA

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revonorcim1nihtiwlevartmm3

norcim5.1nihtiwlevartmm31revo

norcim5.1nihtiwlevartmm31revo

eRuTRePARAeLC,elohdeppatmm9.3

daerht23-8,elohdeppatmm9.3

daerht23-8,elohdeppatmm7

daerht61-61/5,elohdeppatmm7

daerht61-61/5

yTICAPACdAOL lamroNg552 lamroNg552 lamroNg043 lamroNg043

HSINIF dezidonakcalB dezidonakcalB dezidonakcalB dezidonakcalB

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LevART mm3 mm3 mm31 mm31

Miniature Micropositioners

MM3-A adapter





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Mini Micropositioner (MM1 and MM1-3)Single stage measures only 5 × 11 × 26 mm with 3 mm travel . Provides precise and smooth motion with no backlash, positive spring loaded carriage, straight within 1 micron and less than 1 micron maximum wobble . Features fine 80 TPI screw adjustment . 10 mm square mounting surface has a 3 .9 mm tapped center hole for transmission and/or mounting . Available in single X (MM1), X-Y, and X-Y-Z (MM1-3) axis configurations .

Micropositioner (MM3 and MM3-3)Single stage measures only 7 × 17 × 44 mm with 13 mm travel . Offers precise and smooth motion with no backlash, positive spring-loaded carriage, straight within 1 .5 microns, and less than 1 .5 microns maximum wobble . Features fine 80 TPI screw adjustment . 13 mm square mounting surface has a 7 mm tapped center hole for transmission and/or mounting . Available in single X (MM3), X-Y, X-Y-Z (MM3-3) axis configurations .

MM1-Cclamp

MM1-Aadapter

MM3-3

MM1-3

MM1

MM3

Motorized Micropositioner (MM3-M)Designed for applications where working space is limited, our Motorized Micropositioner offers high precision in a turnkey design . The stages are powered by a 10 mm motor with a 10-position magnetic encoder . The 80 TPI lead screw provides smooth and accurate positioning, while still allowing for quick positioning in the coarse mode . Control unit sold separately (see above) .

Stage body (LxWxH): 13.7x1.9x0.91cm

Weight: 68 g

Miniature Micropositioners



MM1 Mini Micropositioner, one axis, 3 mm travelMM1-3 Mini Micropositioner, three axes, 3 mm travelMM1-A Adapter for MM1 and MM1-3, mounted on magnetic Stand MB2MM1-C Clamp for MM1 and MM1-3MM3 Micropositioner, one axis, 13 mm travelMM3-3 Micropositioner, three axes, 13 mm travel MM3-A Adapter for MM3 and MM3-3, mounted on Magnetic Stand MB2MM3-C Clamp for MM3 and MM3-3MM3-M Single axis Motorized Micropositioner MM3-MCPRO Programmable Controller of MM3-M MM3-MSPRO MM3-M Micropositioner & MM3-MCPRO Controller

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Economy Manual Micromanipulator

Vernier scales allow readings to 0 .1 mm . X-axis fine control allows readings to 10 µm .

Left- or right-handed versions of the KITE micromanipulator are supplied with a standard 12 mm clamp and electrode holder M3301EH .

KITEKITE M3301 Manual Micromanipulator

The world’s most widely used micromanipulator

Weighing just 550 grams and employing a slim space-saving design, this well-built micromanipulator outsells all others worldwide for high precision experiments where magnification is in the range of up to 250× . Its design allows units to stand tightly grouped — since all control knobs project to the rear . And because control knobs are clustered within an 8 cm area in a sin-gle vertical plane, resolution is quick — the hand works blindly while the eye monitors the microscopic image . Vernier scales allow readings to 0 .1 mm; x-axis fine control allows readings to 10 microns .

The instrument employs rack-and-pinion drive, V-shaped guideways, and cross roller bearings, so all movement is sure and repeatable, without drift, sideplay, backlash, or sticking . Contact parts are milled of hardened steel for high performance and long life .

Left- or right-handed versions of the M3301 are supplied with a standard 12 mm clamp (M2) and one microelectrode holder (M3301EH) .

Shown on optional M-3 Tilting Base with optional 5-lb Weight #5464

M3301 SPECIFICATIONS TRAVEL RANGE RESOLUTION

X-axis Fine 10 mm 0 .01 mm

X-axis 37 mm 0 .1 mm



SHIPPING WEIGHT 3 lbs (1 .4 kg)

KITE SPECIFICATIONS TRAVEL RANGE RESOLUTION

X-axis Fine 10 mm 0 .01 mm





15873Electrode Holdernot included

KITE-L



KITE-R Kite Manual Manipulator (right-handed) KITE-L Kite Manual Manipulator (left-handed) TB-1 Tilting Base (Optional) Shipping weight: 2 lb (1 kg) KITE-TB-R Kite (right-handed) + Tilting Base ComboKITE-TB-L Kite (left-handed) + Tilting Base Combo

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder (14 cm long) 15873 Optional Angled Electrode Holder (13 cm long) 5464 5-lb Weight for Tilting Base

Shipping weight: 7 lb (3 kg)500475 Ball Joint, 7 cm long, for ∅ 8 mm Holder500476 Ball Joint, 4 cm long, for ∅ 4 mm HolderM4C Microscope Stage Adapter

Also see magnetic stands.

M3301R Manual Manipulator, right-handedM3301L Manual Manipulator, left-handedM-3 80° Tilting Base (Optional) M3301-M3-R Manual Manipulator (right handed) & Tilting Base M3301-M3-L Manual Manipulator (left handed) & Tilting Base 502105 Axis Adjustment Tool



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201

12 mm diameter support arm

Mount a manipulator on your microscope stage

Quick, secure mounting to any microscope stage

(maximum opening: 20 mm).

M4C Microscope Stage Adapter — clamps onto microscope stage to provide a stable support for any manipulator with a ∅ 12 mm clamp.

80 mm

At last — a tilt base you can operate with one hand!

3.5-pound steel base provides additional stability for your micro-manipulator. Holes also allow permanent mounting to your benchtop.

Tilt angle may be set at up to 35°.

Manipulator mounting bracket

included.

Adjust tilt angle easily by simply turning knob of threaded shaft.

Tighten locking screw to lock platform angle.

Two sets of mounting holes are pre-drilled for WPI manipulators (M3301R

shown) but steel platform may be drilled for mounting other devices.

5” x 6” footprint saves space in your work area.

Manipu

lator

not in

clude

d.High Precision,High Stability

6.5

in.

Here’s

a new angle: Ball-joint holder attachment

M5 ∅ 10 mm clamp

M2 ∅12 mm clamp

M6 ∅ 1⁄2-in. clamp

Interchangeable clamps allow manipulators to be mounted on a variety of supports.

7 cm

7.9 mm diam.



TBS Tilt Base with Screw AdjustmentShipping Weight 7 lb

M4C Microscope Stage Adapter

M2 ∅ 12 mm ClampM5 ∅ 10 mm ClampM6 ∅ 1/2-in . Clamp

500475 Ball Joint, 7 cm long, for O .D . 5-9 mm Electrode Holder (shown) 500476 Ball Joint, 4 cm long, for O .D . 2 .8-4 .5 mm Electrode Holder

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HS6 MicromanipulatorEngineered for stability, and built on a twelve-pound steel plate, this

instrument is chosen worldwide for high resolution micro-recording . such as patch clamping, and other research requiring solid, drift-free performance . A superior tool in its own right, HS6 serves equally well as a base for other precision microdrives . HS6 can be bolted directly to a lab fixture or vibration-free platform . Resolution is extremely high — each graduation on its large micrometer barrels indicates just 5 micron movements . Rack and pinion drive, V-shaped guideways, and cross roller bearings give sure, repeatable move-ments without sideplay, slipping, or sticking . All contact parts are milled of hardened steel . A flexible ball-joint assembly allows the electrode to be positioned at any angle relative to the x, y, or z axis . The entire manipulator tilts forward to 25 degrees allowing rapid coarse adjustment of the electrode, and allowing cell penetration along the axis of any of the micrometers . Simple, precise, and durable, the HS6 will provide years of dependable performance .

HS6 SPECIFICATIONS TRAVEL RANGE RESOLUTION

X-axis 25 mm 5 µm

Y-axis 25 mm 5 µm

Z-axis 25 mm 5 µm

SHIPPING WEIGHT 25 lbs (11 kg)

DIMENSIONS 9 .9 x 6 .6 x 9 .9 in . (H x W x L)

Specially adapted for use with the Nanoliter Injector (page 238) for oocyte injection and similar applications, this joystick-controlled micromanipulator allows an easy “steering” motion that translates normal hand movement into smooth submillimeter shifts . Viewed microscopically, movement of the tool-tip corresponds naturally to hand movement, so accurate resolution is intuitive and quick . All fine adjustment can be controlled by the joystick . Pivoting forward, backward, or laterally gives precise x-y adjustment; twisting the joystick adjusts the tool-tip vertically . For added convenience, a separate coarse control lever is also provided for quick raising and lowering . A stop screw—which is set once resolution is achieved—eliminates refocus-ing and streamlines repetitive work by guiding the tip to its previous focussing plane . The stop screw also prevents the tool-tip from being broken during sudden lower-ing and eliminates downward drift—placement is stable enough for even extended use . Because the probe holder tilts a full 90°, the tool-tip pivots easily for precise positioning . Rack-and-pinion drive, V-shaped guideways, and cross roller bearings eliminate backlash, slipping, and sticking . All contact parts milled from hardened steel for precise performance and long life .

Joystick travel: 0 .35 mm to 3 .5-mm, depending on reduction gear ratio setting (adjustable between 1:15 and 1:150) .

M3301EH electrode holder

included

Rock-solid — superb resolution

0° to 90°

MMJR

(right-handed controls)


Joystick-Controlled Micromanipulator

High Resolution Manual Micromanipulators

MMJ SPECIFICATIONS TRAVEL RANGE RESOLUTION



Z-axis 25 mm 0 .1mm

JOYSTICK (X,Y axis) 0 .35~3 .5 mm



included



.

MMJR Joystick Micromanipulator (Right-Handed) MMJL Joystick Micromanipulator (Left-Handed)

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder (14 cm 5 ∅ 7 .2 mm) 15873 Angled Electrode Holder (13 cm long) M4C Microscope Stage Adapter500475 Ball Joint, 7 cm long, for ∅ 8 mm Holder500476 Ball Joint, 4 cm long, for ∅ 4 mm Holder

HS6 MicromanipulatorM3301EH Replacement Electrode Holder (14 cm x ∅ 7 .2 mm) 15873 Optional Angled Electrode Holder (13 cm long)

VFP Vibration-Free PlatformVFW Vibration-Free Workstation



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High Resolution Manual Micromanipulators

The M325 three-axis manual micromanipulator is built of precision micrometer-actuated linear slides . Each slide is comprised of a large micrometer head and a spring-return linear slide . The micromanipulator has been carefully designed to minimize wear in the moving components to achieve a long operational life without the necessity for frequent main-tenance or adjustment . The micrometer head is graduated in 10 micron steps which enable repeatable positioning to an accuracy of ± 2 microns .

A unique spring return mechanism is used to transmit movement of the micrometer spindle to the slide carriage — eliminating backlash, lost motion and reducing thread wear . Each linear slide utilizes ball bearings which enable the M325 to carry loads of up to 1 kg .

The toolholder can clamp onto tools with shaft diameters of 3 .0 mm to 12 .7 mm and allows rotation around two axes . This provides a wide range of options for incorporating the manipulator into your workstations . The M325 can also be configured very easily in left- or right-handed versions to allow several units to be positioned in close proximity .

A quick-release clamp allows easy mounting onto any rod from 10-mm to 12 .7 mm diameter .

A small and compact micromanipulator for manual manipulation in all three axes (x, y and z), the MD4 is equipped with a mounting bracket for a second tool or electrode holder which can be positioned in the x and y axes independent of the manipulator and may also be tilted and swiveled by two fine-adjust screws . Scales allow readings of coarse adjustment with an accuracy of 100 µm . Additional x-axis fine control is achieved with a micrometer screw with a resolution of 10 µm . Supplied with one M3301EH electrode holder and a 12 mm clamp for mounting on M10 Stand or other 12 mm supports . May also be mounted on optional M-3 Tilting Base .

Travel, standard electrode: x-axis, 37 mm (fine, 10 mm); y-axis, 20 mm; z-axis, 25 mm. Additional electrode: x-axis, 7 mm; y-axis, 10 mm.

M-3 Tilting Base not included

MD4L(left-handedcontrols)

Micrometer Slide Micromanipulator

M325 SPECIFICATIONS TRAVEL RANGE RESOLUTION

X-axis 25 mm 10 µm

Y-axis 10 mm 10 µm

Z-axis 10 mm 10 µm


Toolholder rotates around two axes, can hold tools from ∅3.0 mm to ∅12.7 mm.

The M325 can be configured from left- to right-handed version in 60 seconds!

Dual Tool-Holder Micromanipulator

MD4 SPECIFICATIONS TRAVEL RANGE RESOLUTION

X-axis Fine 10 mm 10 µm

X-axis 37 mm 100 µm

Y-axis 20 mm 100 µm

Z-axis 25 mm 100 µm


Mounts onto hori-zontal or vertical rods — any diam-eter from 10 mm to 12.7 mm.


included

M3301EH electrode holders

included



M325 3-Axis Fine Controlled Manual Micromanipulator

OPTIONS AND ACCESSORIESM3301EH Replacement Electrode Holder (14 cm long) 15873 Optional Angled Electrode Holder (13 cm long) 14444 Optional Differential Micrometer Head (per axis) 500475 Ball Joint, 7 cm long, for ∅ 8 mm Holder500476 Ball Joint, 4 cm long, for ∅ 4 mm Holder


Also see Universal Manipulator Stands.

MD4R Double-Holder Micromanipulator (right) MD4L Double-Holder Micromanipulator (left) MD4-M3-R Double-Holder Micromanipulator (right) + Tilting Base MD4-M3-L Double-Holder Micromanipulator (left) + Tilting Base

OPTIONAL ACCESSORIESM3301EH Replacement Electrode Holder (14 cm long) 15873 Optional Angled Electrode Holder (13 cm long) M2 Additional ∅ 12 mm ClampM-3 80° Tilting BaseM4C Microscope Stage AdapterM5 Additional ∅ 10 mm ClampM6 Additional ∅ 1⁄2-in . Clamp 5464 5-lb Weight for Tilting Base* 500475 Ball Joint, 7 cm long, for ∅ 8 mm Holder500476 Ball Joint, 4 cm long, for ∅ 4 mm Holder

*Shipping weight: 8 lb (3.6 kg)


Also see Universal Manipulator Stands.

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SMagnetic Holding Devices

10 mm ∅

10 mm ∅

10 mm ∅

5-6.5 mm ∅

Powerful Ball Joint Rare Earth MagnetUse in constructing your own holding device for small parts/equipment●Smallbutverypowerful:holds2kilograms(~5pounds)!

●Steelballrotatesfreely360°ona180°axis

●M3mountingscrewonballforattachmenttoequipment

●Magnetbasethreaded(M3)formountingontoabaseorequipment

This novel magnetic ball joint has phenomenal holding power for up to 2 kg of attached weight while permitting the ball a full 360° rotation on a 180° axis . You can freely orient your equipment to an infinite number of positions within this rotation . This is made possible by the combination of a steel ball (10 mm diameter) and a powerful rare earth magnet contained in

the magnet cylinder (φ 10 x 20mm) . Convenient M3 attachment sites are provided on



both the ball (male) and the magnet base (female) .

For use with micromanip-ulators for the positioning and holding of optical instruments including various lighting sources and lasers, pipettes and any small parts that would benefit from the flexibility offered by this new magnetic ball joint .

500871 Magnetic Ball Joint

28mm

10mm

M3x12mm

M3x5mm

M1Aprecisionbaseprovidingstablesup-portforsuchdevicesaselectrodesandmanipulators.Adjustablesecondarmadoptsavarietyofangles.(OptionalM510mmclampshouldbeaddedforusewithM3301,DC3001,MD4andMMJmanipulators.)

Base: 50(w)x58(l)x55(h)mm (2.0x2.3x2.2in.)

Vertical Holding Power: 80kgf(176lbforce)

Main Pole: diameter: 12mm(0.47in.) length: 176mm(6.9in.)

Sub Pole: diameter: 10mm(0.39in.) length: 165mm(6.5in.)

Clamp Hole: diameter: 4.5mmand6.5mm

Weight: 1.8kg(4lb)

M1 Magnetic StandM5 10 mm Clamp

M8Aballjointatthebaseofthemainpostallows360°rotation,offeringconsiderableversatility.Thesecondarmadoptsanglesupto75°.(OptionalM510mmclampshouldbeaddedforusewithM3301,DC3001,MD4andMMJmanipulators.)

Magnetic Base: 50(w)x58(l)x55(h)mm (2.0 x2.3x2.2in.)




Clamp Hole: Adjustablefrom4.5mmto6.5mm

Weight: 1.8kg(4lb)

M8 Magnetic StandM5 10 mm Clamp

M1LSamebaseandsupportarmasM1,butequippedwithalonger(14-inch)verticalpost.(OptionalM510mmclampshouldbeaddedforusewithM3301,DC3001,MD4andMMJmanipulators.)

Base: 50(w)x58(l)x55(h)mm (2.0x2.3x2.2in.)


Main Pole: diameter: 12mm(0.47in.) length: 356mm(14in.)


Clamp Hole: diameter: 4.5mmand6.5mm

Weight: 1.8kg(4lb)

M1L Magnetic StandM5 10 mm Clamp

MB2Mechanicalclampingtypetightensthreejointssimultaneouslyjustbyon-toughoperation.Armisfreelyadjustablewithoutdistortion.Equippedwithfineadjusterandmediumsizemagnetforstabilizingthebase.Suitableforperformingprecisionoperation.

Magnetic Base: 30(w)x30(l)x30(h)mm (1.2x1.2x1.2in.)

Vertical Holding Power: 17kgf(37Ibforce)Arm: L1:46mm(1.8in.) L2:46mm(1.8in.) L3:39mm(1.5in.)

Clamp Hole: Adjustablefrom5to6.5mm

Weight: 0.38kg(0.83Ib)

MB2 CompactMagneticStand



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SThe base of each stand exerts a powerful magnetic force that holds it solidly on ferrous metal surfaces — even vertically or upside-down

81⁄2"x12" Steel Base Plate #5052

12 mm ∅ x 25mm

12 mm ∅

12 mm ∅

6-8 mm ∅

12"x24" Steel Base Plate #5479

BASE PLATES: A magnetic stand requires a steel mounting surface . WPI’s steel base plates have plenty of mass to

give stability to your experimental setup . Beveled edges make them easy to handle;

rubber feet hold them off the benchtop, making them easier to grasp when moving;

and the special black coating provides a durable protective finish .

Useful as a probe holder, the M11 is not suitable for heavier items such as micromanipulators.



M9Mechanical clamp tightens three rotatablejoints simultaneously with one lockingknob.Armadjustswithoutdistortion.Baseexerts a magnetic force of 100 kilos forgreateststability.Equippedwithfineadjust-mentforpreciseoperations.



Arms: L1: 119mm(4.7in.) L2: 106mm(4.2in.) L3: 25mm(0.98in.) Ø12mm(0.472in.)

Clamp Hole: none

Weight: 1.8kg(4lb)

M9 Magnetic Stand

M10LSame as M10 but equipped with a taller(14-inch)verticalmainpole.






Weight: 1.8kg(4lb)

M10L Magnetic Stand

M11Bends freely for maximum flexibility. Theconnecting arm twists and bends like asnake.Lockthearminpositionwithaflickofthecontrollinglever.




Sub Pole: none

Clamp Hole: Adjustablefrom6mmto8mm

Weight: 1.4kg(3lb)

M11 Magnetic Stand

M10Similar toM1butwitha12mmdiametersubpole (fits12mmclamp suppliedwithM3301, DC3001, MD4 and MMJ manip-ulators).






Weight: 1.8kg(4lb)

M10 Magnetic Stand

ACCESSORIES5052 Steel base plate, 81⁄2 x 12 in . (10 lb) 5479 Steel base plate, 12 x 24 x 3⁄8-in . (32 lb)

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SMagnetic Holding Devices



501653Anidealaccessoryforopticaltablesandvibration-freeplatform.Reducesexperimen-talset-uptimebyallowingfreeposition-ingandinstantclampdownofopticalcomponents.SwitchableON/OFFmagneticcircuitpermitsfineadjustmentandprecisepositioning.● EasyON/OFFoperationusinglever● Thinandpowerfulmagneticforce● Generousarrayoftapholes

Holding Power: 20kgf(44Ibforce)Dimension: 65(w)x65(l)x20(h)mm 2.6(w)x2.6(l)x0.8(h)in.

Mounting Hole: 8-M4x0.7,depth6mm M8x1,depth6mm Span25mm


501653 MagneticBase

501651Anidealaccessoryforopticaltablesandvibration-freeplatform.Reducesexperimen-talset-uptimebyallowingfreeposition-ingandinstantclampdownofopticalcomponents.SwitchableON/OFFmagneticcircuitpermitsfineadjustmentandprecisepositioning.● EasyON/OFFoperationusinglever● Thinandpowerfulmagneticforce● Generousarrayoftapholes

Holding Power: 20kgf(44Ibforce)Dimension: 75(OD)x20(h)mm 2.9(OD)x0.8(h)in.

Mounting Hole: 4-M4x0.7,depth6mm M8x1,depth6mm Span35mm


501651 MagneticBase

501652MOBITYTMisanewmagneticclampingsystem.Withitseaseofuse,onlyonehandisneededtooperatetheattractivepower.TheMOBITYTMhasastrong88lbfpull,yetweighsonly1.5lbs.MOBITYTMmeetsvari-ousapplicationswith4tappedholesonthetopsurface.

Holding Power: 40kgf(88Ibforce)Dimension: 55(w)x73(l)x50(h)mm 2.2(w)x2.9(l)x2.0(h)in.

Mounting Hole: 3-M4,depth20mm M8,depth15mmWeight: 0.7kg(1.5Ib)

501652 MOBITY Magnetic Clamping System

M7Asmallholderidealforusewherespaceislimited.Mainpostunscrewsfrombasewhichmaytthenbeusedaloneasaswitchablemagneticholder.

Magnetic Base: 30(w)x35(l)x35(h)mm 1.2(w)x1.4(l)x1.4(h)in.

Vertical Holding Power: 20kgf(44Ibforce)Main Pole: Diameter:7mm Length:52mm Screwthreads

Clamp Hole: Diameter:6mmWeight: 0.36kg(0.8Ib)

M7 CompactMagneticStand



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All buildings vibrate — activities of people, machinery, heating and ventilation systems, and nearby truck or rail traffic cause all types of vibrations . These vibrations, though, acceptable to occupants, cannot be tolerated by equipment used in patch clamping, cell injection, analytical balances, and optical microscopes . The short-term effects of such vibrations include inconsistant and unreliable performance . The long-term effects are excessive wear, maintenance, and fatigue failures . In order to protect sensitive instruments and equipment from faulty operation or failure this vibration must be significantly reduced . This can be efficiently accomplished by using Vibration-Free Platform and Vibration-Free Workstation .

VIBRATION-FREE TABLE SPECIFICATIONS VFP VFW

MINIMUM LOAD @ 20 psi

Vertical Natural Frequency 2 .3 Hz 1 .9 Hz

Isolation Efficiency @ 5 Hz 52% 70%


Horizontal Natural Frequency 2 .9 Hz 2 .2 Hz


Isolation Effieciency @ 10 Hz 92% 90%

MAXIMUM LOAD @ 80 psi

Vertical Natural Frequency 2 .1 Hz 1 .5 Hz

Isolation Frequency @ 5 Hz 70% 85%

Isolation Frequency @ 10 Hz 91% 97%

Horizontal Natural Frequency 1 .8 Hz 1 .2 Hz

Isolation Efficiency 81% 91%

Isolation Efficiency 95% 97%

Note: Typical performance efficiencies are for microdistubances

NET LOAD CAPACITY at 80 psi 216 lb (98 kg) 800 lb (363 kg)

Vibration-Free TablesVibration-Free Workstation● Vertical and horizontal vibration isolation

● High performance Active-Air Suspension

● Automatic leveling

● VibraDamped Steel

● Class 100 Cleanroom compatible

● Leveling feet

Vibration-Free Platform● Unmatched price and performance● Low profile Active-Air designs● VibraDamped construction● Low natural frequency● Excellent horizontal isolation● SPILLPRUF spill management● Class 100 Cleanroom compatible

Additional tabletop sizes and finishes are available, as well

as accessories such as side rails and casters.

Call for more information and prices for the

configuration you require.



VFW Vibration-Free Workstation Call

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Surgical MicroscopesPSMB Binocular SurgioScope, floor stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209

PSMT Trinocular SurgioScope, floor stand, video adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209

StereomicroscopesPZMIV Precision Stereo Zoom Microscope (Model IV), Binocular . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210

PZMTIV Precision Stereo Zoom Microscope (Model IV), Trinocular . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210

PZMTIV-CCTV Precision Stereo Zoom Microscope Video System (Model IV) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210

PZMIII Precision Stereo Zoom Microscope (Model III) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 212

PZMTIII Precision Stereo Zoom Trinocular Microscope (Model III) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 212

Inverted MicroscopesINV Binocular Inverted Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214

INV Trinocular Inverted Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214

Upright MicroscopesMOD Multiscope Modular Microscopes, Binocular & Trinocular . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215

Microscope Stage InsertsHeated Stage Insert & Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219

Cooled Stage Insert & Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219

Transparent Heated Stage Insert & Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219

TS-4 Thermoelectric Stage Insert & Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217

Microscope Cameras and Photo/Video AdaptersDigital Eyepiece Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .220

Digital Microscope Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .220

Color CCD Video Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .221

Monochrome CCD Video Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .221

Illuminators and Optical Fiber Light GuideNOVAFLEX Fiber Optic Illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216

MSI-150 Illuminator with diaphragm, filter holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216

MiscellaneousAir-Therm ATX NEW Heater Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218

FluoroDish™ NEW Glass-Bottom Sterile Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76

Cover Slips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77

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Microscopes and Cameras





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WPI’s improved precision SurgioScope (now with five magnification steps) is a portable high quality surgical microscope offering outstanding image quality and value . Incorporating an agile extension arm and excellent working distance objectives, the SurgioScope provides convenient movement and maneuverability necessary for accurate positioning . These important features, together with a high quality optical system, provide sharp image contrast and enhanced large field of vision . The SurgioScope comes fully equipped with a foot-controlled motorized focusing system, normally only found in more expensive surgical microscopes . A unique dual lamp housing enables safe and rapid changing

Precision SurgioScope

Ideal for small animal surgery

SURGIOSCOPE SPECIFICAtIOnSTOTAL MAGNIFICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 .8× — 40×ADJUSTABLE DIOPTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .± 6 Diopter

ADJUSTABLE INTERPUPILLARY DISTANCE . . . . . . . . . . . . . . . . . . . . . . .min . 50 mm — max . 70 mm

WORKING DISTANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 mm — 344 mm

EYEPIECE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 .5x

LENS CHARACTERISTICS

FINE FOCUS ADJUSTMENT RANGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 mm

WORKING HEIGHT (Arm Movement Range Above Floor) 89 cm Post . . . . . . . . . . . . . . . . . . . . . . . . . . Focus on specimens 34 .5" (88 cm) to 51" (130 cm) above floor * 103 cm Post . . . . . . . . . . . . . . . . . . . . . . . . Focus on specimens 40 .5" (103 cm) to 57" (146 cm) above floor ** Subtract Working Distance for height above specimen, 103 cm post recommended for F350 objective.

RANGE OF MOTION Maximum Stretch Radius of Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 870 mm Vertical Movement Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 700-1100 mm Adjustable Range of Small Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 mm

ILLUMINATION Dual lamp housing with quick-change spare and internal coaxial fiber optic cable .

HALOGEN-TUNGSTEN LAMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12V, 100W, with cold reflection

OPTIONAL CAMERA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1/2” CCD camera (e.g., COLCAM)

POWER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110V, 50-60 Hz, or 220V, 50-60 Hz

SHIPPING WEIGHT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 lb (43 kg)

FEAtURES:

● Motorized focusing system, allows hands-free operation .

● Light weight, compact and easy to maneuver . Weighs only 70 lb .

● Dual bulbs prevent illumination failure during surgery .

● Optional video adapter .

● Improved view field .

● Convenient handles .

● Five magnification steps .

ObjectivesObjective

Focal Distance

Working Distance

Total Magnification Field of View

F100 (Optional) 100 mm 94 mm 6 .4× 10× 16× 26× 40×

33 mm, 20 .5 mm, 13 mm, 8 mm, 5 mm

F200 (Included) 200 mm 195 mm 3 .2× 5× 8× 13× 20×

58 mm, 38 mm, 24 mm, 15 mm, 10 mm

F250 (Optional) 250 mm 246 mm 2 .6× 4× 6 .4× 10 .4× 16×

72 .5 mm, 47 .5 mm, 30 mm, 19 mm, 12 .5 mm

F300 (Optional) 300 mm 294 mm 2× 3 .3× 5 .3× 8 .6× 13 .3×

87 mm, 57 mm, 36 mm, 22 .5 mm, 15 mm

F350 (Optional) 350 mm 344 mm 1 .8× 3× 4 .5× 7 .5× 11 .5×

99 mm, 65 mm, 42 mm, 27 mm, 17 mm

Now with 5

magnification

steps!

of the lamp during an operation, without the need to power down . The optional video port on the “T” version permits operational

procedures to be monitored or recorded simultaneously using a video recorder and a COLCAM video camera .



PSMB5 Binocular SurgioScope, F200 objective (Specify post height .) PSMt5 Trinocular SurgioScope, beam splitter, std video adapter, F200 objective (Specify post height.)

See WPI Website for other configurationsSpecify 89 cm or 103 cm post — Specify line voltage

OPtIOnS AnD ACCESSORIES501636 1/2" CS-mount Adaptor (requires Beam Splitter 501637) 501637 Beam splitter501669 Objective lens F100 501638 Objective lens F250501639 Objective lens F300 501640 Objective lens F350 500162 Replacement lamp, 12V, 100 W

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210210

Precision Stereo ZOOM Microscope

The fourth generation of WPI’s precision stereo zoom microscopes uses modular, two parallel beam path design and high quality optical system . The advanced optical design with planachromatic objectives provides sharp and distortion-free contrast image throughout the entire zoom range and comes with an impressive 5-year warranty .

The PZMIV is available in a binocular or trinocular version . In addition, an extensive list of optional accessories is available that makes the PZMIV suitable for integrated optical and biological research .

The microscope comes with a track stand, standard 10x eyepieces (wide-field, distortion-free and high eye point), 1x planachromatic distortion-free objective . See the Table on next page for all optical options .

Shown: PZMtIV with COLCAM and 0.5X c‑mount adapter

(camera and c-mountnot included)

● Modular, two parallel beam design

● Planachromatic objectives, no optical distortion

● High-contrast imaging, ideal for observing

transparent, low-contrast objects

● Large zoom ratio: 8:1

● Large zoom range: 0.62x to 5.0x

● Long working distance

● Step and continuous zoom

● 5-year warranty

All PZMIV and PZMTIV microscopes come with 10x eyepieces, built-in 1x auxiliary lens, and light ring adapter.

502160Lighted Fan Base

COLCAM Video Camera available separately

#500028 Camera Coupler, available separately

Max. Working Distance: 25 cm



PZMIV Precision Stereo Zoom Binocular Microscope (Model IV), on Track Stand PZMIV‑BS PZMIV Microscope on Boom Stand PZMtIV Precision Stereo Zoom Trinocular Microscope (Model IV), on Track Stand PZMtIV‑BS PZMTIV Microscope on Boom Stand PZMtIV‑BCS PZMTIV Microscope on Boom Clamp Stand PZMtIV‑CCtV PZMTIV Microscope Video System, including PZMTIV, COLCAM Color Video Camera, 0 .5x CCD Camera Coupler, Novaflex Optical Illuminator, Bifurcated Optical Fiber Light Guide, and 13” Color TV Monitor (NTSC format)

Please see web site for complete configurations



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PZMIV

PZMtIV‑BS

PZMIV & PZMtIV Eyepieces and Objectives10x Eyepiece 16x Eyepiece 20x Eyepiece 25x Eyepiece

Objective MagField (mm)

(Video Field)Mag

Field (mm)(Video Field)

MagField (mm)

(Video Field)Mag

Field (mm)(Video Field)

Working Distance

0.32x 1.9x - 16x106 -13.1(49.8 - 6.1)

3.2x 25.6x70.6 - 8.8

(49.8 - 6.1)3.9x - 32x

55.4 - 6.9(49.8 - 6.1)

5x - 40x45.4 - 5.6(49.8 - 6.1)

296 mm

0.5x 3.1x - 25x67.7 - 8.4

(31.8 - 3.95)5x - 40x

45.2 - 5.6(31.8 - 3.95)

6.2x - 50x35.5 - 4.4

(31.8 - 3.95)7.8x - 62.8x

20 - 3.6(31.8 - 3.95)

187 mm

0.63x3.9x

- 31.5x53.8 - 6.7

(25.3 - 3.15)6.2x

- 50.4x35.8 - 4.4

(25.3 - 3.15)7.8x -63x

28.2 - 3.5(25.3 - 3.15)

9.8x - 78.8x23 -2.9

(25.3 - 3.15)149 mm

1.0x (inc) 6.2x - 50x33.9 - 4.2

(15.9 - 1.97)9.9x - 90x

22.6 - 2.8(15.9 - 1.97)

12.4x - 100x

17.7 - 2.2(15.9 - 1.97)

15.5x - 125x

14.5 - 1.8(15.9 - 1.97)

80 mm

The Video Field is based on a 1/2-inch CCD (8 mm diagonal) and a 0.5x camera adapter.

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PZMIV SPECIFICAtIOnSEYEPIECES WFH 10×AUXILIARY LENSES 1×ZOOM RANGE 0 .62× - 5×TOTAL MAGNIFICATION 6 .2× - 50×ZOOM RATIO 8 : 1

FIELD OF VIEW ∅33 .9- ∅4 .2 mm

WORKING DISTANCE 80 mm

BINOCULAR TUBE Inclined 45°

INTERPUPILARY DISTANCE 50 – 75 mm

DIOPTER ADJUSTMENT ± 5 Diopter

MICROSCOPE BODY Rotatable 360°

OPTIONAL ACCESSORIES Eyepieces 16×, 20×, 25× Auxiliary lenses 0 .32×, 0 .5×, 0 .63× Total Magnification 1 .9× - 125× Field of view ∅106 - ∅1 .8 mm Working Distance 80 – 296 mm

SHIPPING WEIGHT 23 lb.

CCD Camera Couplers — C-Mount, available as 0.3x, 0.5x and 0.75x.

#502007Articulated Arm



OPtIOnAL ACCESSORIES502000 PZMIV Binocular Body With 10X Eyepieces, 1X Objective, Eye guards502001 PZMTIV Trinocular Body With 10X Eyepieces, 1X Objective, Eye guards502004 Boom Stand W/O Focus Mount (requires 502009 Focus Mount for PZMIV) 502005 Ball Bearing Boom Stand W/O Focus Mount (requires 502009 Focus Mount for PZMIV) 502006 Boom Clamp Stand (requires 502009 Focus Mount for PZMIV) 502007 Articulated Arm and Table Clamp w/o Focus Mount (requires 502009 Focus Mount for PZMIV) 502009 Universal Focus Mount for 76 mm PZMIV (Required for BS, AAC, BBS, and BCS) (5/8” pin) 502160 Lighted Fan Base, Fluorescent base, Tungsten Halogen beam502010 10X Wide Field Eyepiece for PZMIV (pair) 502011 16X Wide Field Eyepiece for PZMIV (pair) 502012 20X Wide Field Eyepiece for PZMIV (pair) 502013 25X Wide Field Eyepiece for PZMIV (pair) 502015 Ring Light Adapter for PZMIV (For R-8-8-WPI01 Ring Light Guide) 502016 0 .32x, Planachromatic Objective (Distortion-free) (296 mm WD) 502017 0 .50x, Planachromatic Objective (Distortion-free) (187 mm WD) 502018 0 .63x, Planachromatic Objective (Distortion-free) (149 mm WD) 502019 1 .0x, Planachromatic Objective (Distortion-free) (80mm WD) 500261 0 .3x CCD Camera Coupler, C-Mount (Use with Pixera Professional) 500262 0 .5x CCD Camera Coupler, C-Mount (Use with COLCAM) 500263 0 .75x CCD Camera Coupler, C-Mount500028 1 .0x CCD Camera Coupler, C-Mount (Use with Digital Camera Adapter Kit 501384) 501384 Digital Camera Adapter Kit (see page 222 for details) 500828 Stage Micrometer, 1 mm/200 div (recommended for calibrated photography) 502163 Wall Mount Plate for Articulated Arm SystemnOVA NOVAFLEX Fiber Optic Illuminator500186 Bifurcated Light Guide with LensesR‑8‑8‑WPI01 Ring Light GuidenOVA‑186 NOVAFLEX Fiber Optic Illuminator with Bifurcated Light Guide and LensesEJA Replacement Lamp for NOVAFLEX502167 Replacement Fluorescent lamp for Lighted Fan Base (502160) 502168 Replacement Tungsten Halogen lamp for Lighted Fan Base (502160)

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Precision Stereo ZOOM Microscope

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WPI’s third-generation stereomicroscope, PZMIII, is an ideal tool for tissue dissection, cell injection, specimen manipulation, electrode inspection, and many other applications that require a magnified, stereo viewing and ample working distance . It offers the leading brand’s quality and performance at an affordable price . Advanced optics provide the sharpest image that can only be found among the best of this class . It is superior to many stereomicroscopes costing almost twice as much . Zooming is achieved by a spring-loaded nub that is smooth and effortless . The compact size and light weight make it more stable and easily manipulated on the boom stand . A specially designed photo/video module can easily upgrade the binocular PZMIII to a trinocular microscope at any time for photo, video, or digital imaging . In addition, an extensive list of optional accessories is available that can make the PZMIII suitable for almost any bio-research applications requiring a stereomicroscope . See next page for options (if the item you seek is not listed, please call WPI) .

PZMtIII‑CCtV(ntSC format)

Quality and

Precision to ImproveYour Vision

PZMIII

Actual base wider than pictured here. See

example at left.



PZMIII Precision Stereo Zoom Microscope (Model III), on Post Stand PZMIII‑BS PZMIII Microscope on Boom Stand PZMIII‑AAC PZMIII Microscope on Articulated Arm with Table Clamp PZMtIII Precision Stereo Zoom Trinocular Microscope (Model III) PZMtIII‑CCtV PZMTIII Microscope Video System, including PZMTIII, COLCAM Color Video Camera, 0 .5X CCD Camera Coupler, Novaflex Optical Illuminator, Bifurcated Optical Fiber Light Guide, and 13" color TV monitor (NTSC format) PZMtIII‑BS PZMTIII Microscope on Boom Stand PZMtIII‑AAC PZMTIII Microscope on Articulated Arm with Table Clamp PZMTIII-BS-CCTV PZMTIII Microscope, Boom Stand, Video System PZMTIII-AAC-CCTV PZMTIII Microscope, Articulated Arm, Video System

All PZMIII and PZMTIII microscopes come with 10x eyepieces and built-in 1x auxiliary lens.



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PZMIII Eyepieces and Objectives10x Eyepiece 15x Eyepiece 20x Eyepiece 25x Eyepiece

Objective Mag.Field (mm)

(Video Field)*Mag.

Field (mm)(Video Field)*

Mag.Field (mm)

(Video Field)*Mag.

Field (mm)(Video Field)*

Working Distance (mm)

0 .3x 2x - 13 .5x114 - 17 (53.6 - 8)

3x - 20 .3x84 -13

(53.6 - 8)4x -27x

69 - 10 .3(53.6 - 8)

5x - 33 .8x44 .8 - 6 .7(53.6 - 8)

287 mm

0 .4x 2 .7x - 18x86 - 13

(40.4 - 6.1)4x - 27x

63 - 9(40.4 - 6.1)

5 .4x - 36x52 - 7 .7

(40.4 - 6.1)6 .7x - 45x

33 .6 - 5 .0(40.4 - 6.1)

217 mm

0 .5x 3 .4x - 22 .5x69 - 10

(32.4 - 4.7)5x - 33 .8x

51 - 7(32.4 - 4.7)

6 .7x - 45x42 - 6 .2

(32.4 - 4.7)8 .4x - 56 .3x

26 .9 - 4 .0(32.4 - 4.7)

177 mm

0 .75x 5x - 33 .8x45 - 7

(21.1 - 3.3)7 .5x - 50 .6x

34 - 5(21.1 - 3.3)

10x - 67 .5x28 - 4 .2

(21.1 - 3.3)12 .6x - 84 .4x

17 .9 - 2 .7(21.1 - 3.3)

117 mm

1 .0x 6 .7x - 45x34 - 5

(16 - 4.7)10x - 67 .5x

25 - 3 .7(16 - 4.7)

13 .4x - 90x21 - 3 .1(16 - 4.7)

16 .8x - 112 .5x13 .4 - 2 .0(16 - 4.7)

100 mm

1 .5x 10x - 67 .5x23 - 3 .4

(10.8 - 1.6)15x - 101 .3x

17 - 2 .5(10.8 - 1.6)

20 .1x - 135x14 - 2 .1

(10.8 - 1.6)25 .1x - 168 .8x

9 .0 - 1 .3(10.8 - 1.6)

47 mm

2 .0x 13 .4x - 90x12 - 2 .5

(5.6 - 1.17)20 .1x - 135x

13 - 1 .8(5.6 - 1.17)

26 .8x - 180x10 - 1 .5

(5.6 - 1.17)33 .5x - 225x

6 .7 - 1 .0(5.6 - 1.17)

26 mm

* The video field of view is based on a 1/2-inch (8 mm diagonal) CCD camera and a 0.5x camera adapter.

#5013810.5x C-mount

CCD Camera coupler — Use

with cameras which have 0.5-in. CCD.

qwertyuiopas

PZMIII‑BS

PZMIII SPECIFICAtIOnSEYEPIECES WFH 10xZOOM RANGE 0.67x - 4.5xTOTAL MAGNIFICATION 6.77x - 45xFIELD OF VIEW ∅ 34 MM - ∅ 5 MMWORKING DISTANCE 100 mmBINOCULAR TUBE Inclined 45°INTERPUPILLARY DISTANCE Adjustable 47-70 mmDIOPTER ADJUSTMENT ±5 Diopter (both eyepiece tubes)MICROSCOPE BODY Rotatable 360°AUXILIARY LENSES Total magnification 2x - 225x Biggest Field of View ∅ 110 mm Working Distance 26-287 mm SHIPPING WEIGHT 23 lb.

PZMIII

#502163 Wall-Mount Plate — Mount the microscope on the

wall for convenient storage when space is tight.

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#502005 Ball Bearing Boom Stand — Dual Arm Ball Bearing Boom

Stand for increased stability and smoother horizontal movements.

#503051 Manual Stage — Mounts in the circular opening in the PZMiii base. XY travel distance: 75 x 56 mm. Glass size: 116 x 96 mm. Active diameter: 37.6 mm. Dimensions: 180 x 155 x 27 mm. Fits this base only.

#502007Articulated Arm

40 cm



OPtIOnAL ACCESSORIES501352 PZMIII Binocular Body, pair of 10x eyepieces and eyeguards501353 Fan Post Stand with 76mm Focus Mount502009 Universal Focus Mount, 76 mm ID for PZMIII Body502004 Boom Stand without Focus Mount502005 Ball Bearing Boom Stand without Focus Mount502007 Articulated Arm with Wall-Mount Plate, without Focus Mount 502163 Wall-Mount Plate, 6” x 6” (or 15 .24 cm x 15 .24 cm) 501367 Universal Focus Mount, 83mm ID for PZM Body501369 Wide Field 10x Eyepieces (pair) 501370 Wide Field 15x Eyepieces (pair) 501371 Wide Field 20x Eyepieces (pair) 501372 Wide Field 25x Eyepieces (pair) 501373 0 .3X Long Working Distance Objective Lens501375 0 .5x Long Working Distance Objective Lens501376 0 .75x Long Working Distance Objective Lens501377 1 .5x Long Working Distance Objective Lens501378 2 .0x Long Working Distance Objective Lens501379 PZMTIII Trinocular Body, pair of 10x eyepieces and eye guards501381 0 .5x C-Mount CCD Camera Coupler501384 Digital Camera Adapter Kit 13338 Ring Light Adapter for PZMIII Series503051 Manual Stage for PZMiii RtCL‑10 Reticle in 10x Eyepiece, 0-90°, 200 divisions per mmRtCL‑20 Reticle in 20x Eyepiece, 0-90°, 200 divisions per mm

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The most durable, easy-to-use microscope for inverted applications and long working distance optics

The most durable, easy-to-use microscope for inverted applications and long working

distance optics The exclusive design utilizes interchangeable sliders, thus allowing a researcher to use the same

set of objectives for Bright Field, Phase Contrast and Nomarski DIC examination of living cells, cultures, sediments, precipitates

and other specimens . This also eliminates the need for costly second and third sets of

objectives, making this microscope both versatile and economical . The microscopes come loaded with valuable extras that would cost hundreds of dollars from any other

manufacturer . Start using your microscope right from the box!

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• Bright Field

• Phase Contrast (10x or 20x)

• Nomarski DIC (with IQ902DIC, IQ903DIC)

• Long Working Distance Objectives (corrected for 2.5 mm vessels)

• 10x and 15X eyepieces

• 10 micron Micrometer reticle (15X eyepiece)

• Set of six high-quality tinted filters

• Set of polarization filters

• Centering telescope

• Replacement lamp

• Stage inserts and extension plates

• 35mm Film Camera

• Lifetime warranty

OBJECtIVES

type Magnificationnumerical Aperture

Working Distance (mm)

Contrast techniqueBF PH POL DIC

tQA‑Series Plan Achromats

2 .5X 0 .08 20 .0 t t6 .3X 0 .20 19 .0 t t10X 0 .22 15 .0 t t t t20X 0 .45 3 .0 t t t t

Mechanical Tube Length: 160mm; Parfocal Distance: 45mm; Glass Thickness: O-2 .5mm .

Inverted Microscope

For complete Specifications, go online and visit WPI's website —

www.wpiinc.com



WPI InVERtED MICROSCOPEInV‑IQ902 Binocular Inverted Microscope, BF, PH, POLInV‑IQ903 Trinocular Inverted Microscope, BF, PH, POLInV‑IQ902DIC Binocular Inverted Microscope, BF, PH, POL, DICInV‑IQ903DIC Trinocular Inverted Microscope, BF, PH, POL, DIC

OPtIOnAL ACCESSORIES500811 Trinocular Head500812 Video (C-Mount) Adapter for 500811500813 Vertical Eyepiece Tube Adapter (for video adapters 502161 & 502162) 500814 Photo Tube (T-Mount) Adapter for 500811500816 Replacement Bulb, Halogen, 12V / 30W500821 Eyepiece, 15x, wide field, F .N . 11500825 Teaching Pointer Eyepiece with Eyecup, 10x500826 Dual-View Teaching Attachment, includes 500825500827 Micrometer Eyepiece, 10x, 10 mm / 100 div . 500828 Stage Micrometer, Reflected Light, 1 mm / 200 div . 500278 0 .5x C-Mount—C-Mount Reducer502162 0 .5x C-mount (for 1⁄2” 8mm CCD cameras, use with 500813) 502161 0 .3x C-mount (for 1/3” 6mm CCD cameras, use with 500813) 502166 Micrometer Eyepiece, 15x, F .N . 11 (10 mm / 100 div)


Shipping Weight: 94 lb (43 kg) — 40x42x30” (102x107x76 cm)



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SMULtISCOPE™ Modular MicroscopeThe most versatile, durable and affordable Bright Field Microscope on the market

Rugged construction for years of reliable service

backed by a limited five-year manufacturer’s warranty

MODULAR MICROSCOPE FEATURES AND SPECIFICATIONSOPTICAL SYSTEM 160mm Mechanical Tube Length, 33mm Parfocal Distance, 28X–1000X Magnification for observation

HEADBinocular 45° inclined, Trinocular 30° inclined, 360° rotatable . Magnification: 1 .1X, 1 .6X, 2 .5X . IPD adjustment range: 55mm-75mm . DA (left eyepiece) ±5dpt

NOSEPIECE Quadruple, aligned for parfocality and parcentricity

STAGE (BF) Mechanical stage, X/Y controls . Size: 135mm x 120mm . Specimen cross-travel: 80mm x 40mm . Scale graduation: 1mm X 0 .1mm

CONDENSER Brightfield, N .A . 1 .25, Abbe type . Spherically corrected

ILLUMINATION Variable Halogen, 12V/20W Internal heat-absorbing filter . External power supply .

OBJECTIVE (BF) EAB Achromats, 4X 0 .12, 10X 0 .25, 40X 0 .65 (spring loaded), 100X 1 .25 Oil Immersion (spring loaded)

EYEPIECE 10X, F .N . 18 Widefield . High eyepoint ESW Series

FILTERS 33mm Diffusing, 33mm Blue

Also included: Instruction manual, dust cover, spare bulb, immersion oil (Type A, 1/4 oz)

BrightfieldMOD-BMH-BF Brightfield Trinocular

MOD-TMH-BF-V

● Modular design allows you to configure your microscope to suit your needs

● Unique Fine Wheel enables you to quickly bring specimens into sharp focus



WPI MODULAR MICROSCOPEMOD‑BMH‑BF Binocular, M-Stage, Brightfield, Mechanical X-Y stageMOD‑tMH‑BF‑V Trinocular- Video, M-Stage, Brightfield, Mechanical X-Y stage

Shipping info: 20 lbs (9kg); 21x16x16 (53.5x41x41cm)

OPtIOnAL ACCESSORIES500811 Modular Trinocular Head 500812 1X Video (C-Mount) Adapter for 500811500813 Vertical Eyepiece Tube Adaptor (for Video adaptors 502161 & 502162) 500814 Photo Tube (T-Mount) Adapter for 500811500815 Replacement Bulb for MOD microscopes, 6V / 20W500821 Eyepiece, 15x, wide field, F.N. 11

500822 Objective, 20x, 0.40, Achromatic, Brightfield500823 Objective, 60x, 1.0-0.70, Achromatic, Oil, Brightfield500827 Micrometer Eyepiece, 10x, 10 mm/100 div. 500828 Stage Micrometer, Reflected Light, 1 mm/200 div. 502162 0.5X C-mount (for 1⁄2” (8mm) CCD Camera, use with 500813502161 0.3X C-mount (for 1/3” (6mm) CCD Camera, use with 500813

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The NOVAFLEX Fiber Optic Illuminator provides reliable, uninterrupted high-intensity light for microscopes and fiber optic spectrometers . The most widely used halogen light source, NOVAFLEX allows a continuous range of subdued or concentrated lighting controlled by a rotary dimmer on the front panel . NOVAFLEX may be used with a ring light and single or bifurcated flexible fiber bundles, enabling the light beam to be placed exactly where needed . Forced air cooling prolongs lamp life . EJA lamp color temperature is 3350°K . An interlock switch automatically cuts off power when front panel is opened to replace bulb . Rugged aluminum case .

Size: 24 x 13 x 16 cm (9.375 x 4.75 x 6.25 in.)

Weight: 4.8 kg (9 lb)

High intensity source for fiber

optic illumination

This versatile 150 W halogen high-intensity fiber optic illuminator features a quick change lamp module for easy access and fast replacement . A solid state dimmer adjusts voltage, extends lamp life, and changes color temperature . A mechanical diaphragm allows color temperature to remain constant as light output changes . EJA lamp (included) provides optimum light output . Light guides available separately .

MSI‑150 SPECIFICAtIOnSLAMP TYPE EJA (150 W)

INPUT VOLTAGE 115 V or 230 V

INPUT FREQUENCY 50/60 Hz

OUTPUT LAMP VOLTAGE 21 VAC

LAMP COLOR TEMPERATURE 3350K

Fiber Optic Illuminator controls light color and intensity

Flexible Light Guides SI‑40‑8 and SI‑72‑8, with 8 mm diam‑eter fiber and non‑metallic sheath, are suitable for use in a Faraday cage. (Lens L‑32‑32 available separately.)

Ring Light R‑8‑8‑WPI01 can be used with PZM Stereo Microscope for shadow‑free illumination. 18‑in. (46 cm) flexible cable

Filter holder accepts two 20 mm x 2 mm filters (not incuded).

Light guides available separately

Bifurcated Light Guide 500186

available separately

L‑32‑32

ID: 58 mm

21" (53 cm)



nOVA NOVAFLEX Fiber Optic Illuminator (115 V, 60 Hz) nOVA‑Z NOVAFLEX Fiber Optic Illuminator (230 V, 50 Hz) MSI‑150 Illuminator w/ diaphragm, filter holder (115 V, 60 Hz) MSI‑150Z Illuminator w/ diaphragm, filter holder (230 V, 50 Hz) nOVA‑186 NOVAFLEX Illuminator & Bifurcated Light Guide

LIGHt GUIDES AnD ACCESSORIES500186 Bifurcated Light Guide (with lenses) R‑8‑8‑WPI01 Ring Light Guide for PZM and PZMIII Series*

*Requires adapter #13338 for use with PZM, PZMII and PZMIII.

13338* Ring Light Adapter (48 mm ∅) for PZM, PZMII, PZMIII502015* Ring Light Adapter for PZMIVSI‑72‑8 Flexible Light Guide, 72 in . (183 cm) Requires L-32-32 SI‑40‑8 Flexible Light Guide, 40 in . (102 cm) Requires L-32-32 L‑32‑32 Lens for SI-40-8 and SI-72-8St‑HA Infrared Blocking Filter for MSI-1505475 Adapter for SMA-terminated Fiber Optic CablesEJA Replacement Lamp, 150 Watt* A ring light adapter is included with each PZMIII and PZMIV microscope.



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TS-4 is a thermoelectric (Peltier) device and has no moving parts . Only AC current and a trickle of running water are needed for operation . Available in two versions (TS-4, range -20° to +60°C, and TS-4ER, range -20° to +100°C), the device consists of a stage, a controller, and an optional pump unit .

The stage is a thin, nickel-coated copper plate with a thermoelectric module mounted on one side . The plastic base is the same size and thickness as a glass slide and fits onto

thermoelectric Stage Insertthe mechanical stage of the microscope in the same manner as a slide . The thermal stage can therefore be moved relative to the optics by the normal mechanical stage controls . A hole in the metal plate permits the use of transmitted light .

Water is required for removal of excess heat from the stage . The two water tubes have self-sealing connections to prevent spills . Running water from a tap can be used . If tap water is not easily accessible, the optional pump and tank unit is compact and convenient .

tS‑4 SPECIFICAtIOnSSTAGE 11/4 x 11/2 in . (32 x 38 mm)

TEMP . CONTROLLED SURFACE 16 oz . (454 g) including leads

WEIGHT Copper, nickel plated, 5 ft (1 .5 m)

LEAD

CONTROLLER

TEMP . RANGE -22° C to +60° C

RESOLUTION 0 .1°C

POWER CONSUMPTION Approx . 100 Watts

DIMENSIONS 8 x 7 x 17 in . (20 x 18 x 43 cm)

WEIGHT 17 lb (7 .7 kg)

THERMOMETER

TEMP . RANGE -100° C to +200° C

RESOLUTION 0 .1° C

SENSOR Type T thermocouple

● Holds specimen at any temperature, from -20° to 100°C

● Digital display of set-up and operating temperatures

● Control to within 0.1°C

heating and cooling . During set-up the digital display indicates the control temperature being selected . The actual temperature is displayed during operation .

The thermometer circuitry in the TS-4 can also be used as an independent instrument, either with the microprobe supplied or with any type T thermocouple sensor . A switch on the front panel allows easy selection of the external sensor . Range of the thermometer is -100° to +200°C .

TS-4LPD — Designed to accomo-date a 60mm or 100mm petri dish. Range: -5 to 60 °C.

TS-4SPD — Designed to accomodate a 35mm petri dish. Collar may be removed for temperature controlled slide applications. Range: -15 to 60 °C (TS-4SPDER: -15 to 85 °C).

For more configuration options, please see the WPI Web site or contact [email protected]

Sensors mounted on the stage provide temperature monitoring for control purposes and safety . The safety sensors prevent overheating in case of failure of cooling water supply or other malfunction .

The controller produces a maximum of 10 Amps at approximately 5 volts DC for the thermoelectric modules in the stage . Output polarity is automatically reversed when the stage requires a change from



TS-4 Thermal Microscope Stage & ControllerTS-4ER Thermal Microscope Stage & Controller (extended range) TPC-4/PTU Thermal Microscope Stage with Pump & Tank UnitTPC-4ER/PTU Thermal Microscope Stage (extended range) w/ Pump & Tank UnitTPC-4SPD Thermal Microscope Stage, accommodates 35 mm petri dishTS-4SPDER Thermal Microscope Stage (extended range) 35 mm petri dish TPC-4LPD Thermal Microscope Stage, accommodates 100 mm petri dish


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Maintenance of the desired temperature set-point can now be achieved through the use of a precision, shielded temperature probe coupled to a peripheral Air-Therm heating unit .

Precision: Noise is a major concern in any experimental application . WPI's Air-Therm™ is electrically quiet as well as acoustically quiet) . Since the Air-Therm can be placed away from the incubator, any residual electrical and vibrational interference from the heater is virtually eliminated . Accuracy of ±0 .1°C can be routinely maintained at the sample site (using a fully programmable differential algorithm to automatically adjust the temperature on the Air-Therm unit) . Air-Therm maintains a uniform temperature across the microscope stage (allowing for uniform heating of multiwell dishes) . These tight tolerances result in minimal focal drift once sample/incubator equilibrium has been achieved . Furthermore, because of the airflow pattern and temperature uniformity, the incubator temperature rapidly readjusts following the opening of incubator doors (e.g., during sample changing/manipulation or microscope hardware manipulation) .

Glass Bottom Dishes: For high numerical aperture (NA) live-cell imaging, cells must be plated on glass (e.g., WPI’s FluoroDish Glass Bottom Dishes, spotting 0 .17 mm glass thickness) or standard cover slips . Since cover slips inherit other problems associated with sterility and accidental damage to expensive microscope condensers, WPI designed new and improved glass bottom dishes (35 mm x 10 mm or 35 mm X 22 mm) that are not cytotoxic . A special adhesive in conjunction with an advanced dish design makes the FluoroDish the only non-cytotoxic glass dish in the world . See page 76. This means that cells can actually be cultured for 4 to 5 days in the same dish and then carry the complete cell culture with media from the incubator directly into

the (Air-Therm controlled) recording chamber . This is a comfort level that hitherto was unattainable by scientists and clinical researchers since cells will have to be transported from the culture dishes on to cover slips just prior to imagery or attaching patch electrodes .

Chamber: The most problematic aspect of any live cell imaging procedure or patch clamp approach is the thermal drift of high magnification lenses caused by temperature fluctuations in the microscope's environment . It is necessary to constantly readjust the focus during image acquisition due to this thermal-drift, introducing artifacts during quantitative microscopy due to the photo-bleaching that occurs during refocusing . The optimal solution to this problem is to use WPI’s Air-Therm temperature controller connected to a Plexiglas or acrylic chamber that encloses the entire imaging area (or at least the objectives and the stage) . Using such a chamber will result in highly stable temperature control and minimal thermal drift .

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Precision Heater ControllerSmart, electrically quiet air heater for live cell

imaging systems and custom incubators

AIR‑tHERM SPECIFICAtIOnSAIR FLOW RATE 20 to 50 CFM (0 .55 to 1 .4 cubic meter/M)

CONTROL TEMP . RANGE Ambient to 60°C

TEMPERATURE RESOLUTION 0 .1 °C

TEMPERATURE ACCURACY 0 .2 °C

CONTROL MODES (a) Auto (PID set manually, or Self-Tune) (b) Manual tune

ANALOG OUTPUT 0 .5°C resolution FOR CHART RECORDER 0-10V, 0 to 100 C (default, adjustable via controller)

HEATING VOLUME More than 4 cu ft (85 liter), non-circulating

POWER (CE) 300 W, 95-135 V or 220-240 V, 50/60 Hz

DIMENSIONS 61⁄2 x 8 x 71⁄2 in . (15 .5 x 21 x 19 cm)

SHIPPING WEIGHT 10 lb (4 .5 kg)



AIRtHERMY‑AtX Air-Therm Heater 120 V, U .S . AIRtHERMZ‑AtX Air-Therm Heater 240 V Specify line cord2 pieces of 4.5 ft clear, coil-reinforced heater hose included with Air-Therm.

Specify Line (Mains) Voltage

OPtIOnAL ACCESSORIES 15590 Clear hose, 2 .5” diam ., 4 .5 ft300276 Replacement platinum temperature probe3491 5 ft (1 .5m) probe extension cable



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Accurate and reliable specimen temperature control

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Heated stage insert● 24 heating elements arranged in parallel for very high reliability

● High thermal conductivity material for uniform temperature distribution

● Direct contact bewteen heated insert surface and petri dish/slide

● All solid-state electronics for long-life

The heated stage insert maintains the temperature of a petri dish or slide on a microscope from ambient to 50ºC ±0 .1ºC . A 24-heating element array maintains the temperature of the stage .

The cooled inserted stage achieves ±3ºC from ambient down to 10ºC . A Peltier “heat pump” maintains the temperature of the stage . The heat is removed from the cooled insert stage by a water cooled heat exhanger that requires a water supply with an 8-mL/min flow rate .

These two stages will fit most inverted and standard microscopes with circular inserts .

Cooled stage insert● Accurate, reliable temperature control using peltier “heat pump”

● Controller can be tuned to achieve ±3ºC from ambient down to 10ºC

● High thermal conductivity material for uniform temperature distribution

● All solid-state electronics for long-life

COOLED StAGE SPECIFICAtIOnSTEMPERATURE Ambient to 10ºC

ACCURACY ±0 .1ºC

POWER 115/230 VAC, 50/60 Hz

COOLED STAGE INSERT Thickness 3 mm thick OD Dependent on microscope

CONTROLLER DIMENSIONS 75 x 200 x 140 mm H x W x D (2 .3 x 7 .9 x 5 .5 in)

WEIGHT 0 .65 kg (1 .4 lb)

HEAtED StAGE SPECIFICAtIOnSTEMPERATURE Ambient to 50ºC

ACCURACY ±0 .1ºC

POWER 115/230 VAC, 50/60 Hz

HEATED STAGE INSERT Thickness 3 mm thick OD Dependent on microscope

CONTROLLER DIMENSIONS 75 x 200 x 140 mm H x W x D (2 .3 x 7 .9 x 5 .5 in)

WEIGHT 0 .65 kg (1 .4 lb)

Heated Stage Inserts have the following standard options:

Diameter 110 mm for Olympus IX, IMT2 108 mm for Nikon Diaphot, Eclipse 100 mm for WPI Inverted Microscope

Aperture 6 mm, 16 mm

Cooled Stage Inserts have the following standard options:

Diameter 108 mm for Nikon Diaphot, Eclipse Aperture 6 mm, 16 mm

transparent Heated Stage InsertThe transparent heated stage allows better visibility of the stage area . The heating element is incorporated in transparent glass with see-through area of 80 mm in diameter . Temperature control box is included with the stage .

TEMPERATURE RANGE Ambient to ~ 40 deg C

TEMPERATURE ACCURACY Stability 0 .1 deg C

ACCURACY 0 .5 deg C (user adjustable offsets for calibration in situ)

POWER 24V, ~10W

HEATED STAGE THICKNESS 0 .8 mm glass

SEE-THROUGH AREA 80 mm diameter



502032 Transparent Heated Stage insert, 108 mm diam ., for microscope stage mounting, including temperature control box 502033 Transparent Heated Stage insert, 110 mm diam ., for microscope stage mounting, including temperature control box 502034 Transparent Heated Stage insert, 110 mm diam ., for bench mounting, including raised stand* and temperature control box

* Specify required height of stand

When ordering, please specify your choices from the standard options above by replacing “xxx” with the diameter of insert and YY with diameter of aperture hole in millimeters. In the order number, “BE” represents bottom entry wires for microscope mounting, and “SE” represents side entry wires for bench mounting.

14417-F-xxx-yy Heated Stage Insert & Controller 14417-F-xxx-yy-SE Heated Stage Insert & Controller 500820-F-xxx-yy Cooled Stage Insert & Controller


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Digital Eyepiece Cameras provide an easy and economical solution for capturing digital video from the microscope and recording it in your computer . The image is taken from the ocular port, so there is no need for the microscope to have a separate photo port . It can be used on any type of microscope . Since the camera connects directly to the computer’s USB port, no video capture card is necessary . The diameter of the field of view in the primary image plane for the digital camera is up to 18 mm .

ScopePhoto software aids in analysis of the captured images .

● Image archival with data attachment (* .PTL format, other formats are also supported) .

● Image capture through VFW and Twain interface .

● Image cut-&-paste from image to image, image to file .

● Image basic operations like other similar software are included in the Image menu .

● Image layer operation specially designed for the measurement operation .

Digital Eyepiece Camera

DIGItAL CAMERA SPECIFICAtIOnSDEC‑18 USBCAM‑3M

IMAGE SENSOR 1/3" CMOS 350K pixels 1/2" CMOS, 3 .1 M pixelsMAX RESOLUTION (still) 640 x 480 (Still) 2048 x 1536, 1024 x 768

MAX RESOLUTION (motion) 640 x 480, 15 fps; 320 x 240, 30 fps(motion) 2048 x 1536 @ 7 .5 fps; 1024 x 768 @ 20 fps

VIEW FIELD Inscribed rectangle of ∅18 mm Inscribed rectangle of ∅18 mmUSAGE Ocular-tube, photo-tube C-mount onlyDYNAMIC RANGE >52 dBSENSITIVITY 2 .7 v/lux-sec@green

1 v/lux-sec2 .1 v/lux-sec@red2 .0 v/lux-sec@blue

FILE FORMAT BMP, TIFF, JPG, PICT, PTL BMP, TIFF, JPG EXPOSURE Automatic Automatic/ManualSHUTTER CONTROL Automatic Automatic/ManualINTERFACE USB cable of length 2 m USB cable of length 2 mSYSTEM REQUIREMENT Windows XP Windows XP, Service Pack 2

SOFTWAREDriver for USB 1 .0, compatible with USB 2 .0; ScopePhoto software

Driver for USB 2 .0; XLICap software

WEIGHT 4 .8 oz without eyepiece adapter 4 .8 ozCAMERA SIZE Cylindrical, @ 50 mm 86 x 30 x 60 mmCAMERA BODY Anodized aluminum Anodized aluminumACCESSORIES 30 mm, 30 .5 mm adapter C-mount required on microscopeWHITE BALANCE Tungsten, Fluorescent Tungsten, Fluorescent

DEC‑18

USB CAM‑3MColour CMOS QXGA (3.1 Mpixel) Camera

This high performance camera incorporates a half-inch 2048 x 1536 (QXGA) image array and an on-chip 10-bit A/D convertor capable of operating up to 7 .5 frames per second (fps) with full resolution and 20 fps at XGA (1024 x 768) resolution . The camera uses USB 2 .0 connectivity to transfer images to the user’s computer . The simple plug-and-play software allows the resolution to be changed easily .

● Optical black level calibration ● Video or snapshot operations

● Auto Exposure ● Auto Gain Control ● Auto White Balance

● Variable Frame Rate

● On-chip R/G/B channel and luminence average counter

● Power on reset and power down mode ● Integral IR filter

● USB 2 .0 “B” type connector ● Standard adjustable C-mount

Includes XLICap

software — check for

details online

XLICap software has many unique features for still and live image capture● Simple layout with toolbar for common

functions

● Pan and Zoom with selectable magnification

● Selectable image Resolutions

● Mirror / Flip / Rotate Image options

● Save images in a variety of formats

● Record live videos

● Email a screen shot instantly

● On-screen annotation and time/date stamping

● Freeze the image at any time

● Copy to clipboard and paste into other applications

● Automatic one-touch white balance

● Colour or black and white mode

● Save and restore Custom Preference Profiles

Digital Microscope Camera



USBCAM‑3M 3 .1 Megapixel Digital Microscope CameraDEC‑18 Digital Eyepiece Camera



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● Sensitivity of 0 .2 lux @ f1 .2

● Resolution of 470 TV lines

● No burn in, no image lag, no geometric distortion .

● Magnetically shielded .

● Composite video out at 1 Vpp @ 75 ohms .

● Power consumption under <2W @ 12VDC

● S/N ratio 52 dB, typ ., 60 dB max .

● Gamma of .45 .

● Operating temperature range of –20 to 50°C .

● White balance color temperature switchable

● Includes standard tripod mount, power supply, 6-ft (~2m) BNC cable, RCA adaptor .

● Sensitivity of 0 .05 lux @ f1 .2

● Resolution is 600 horizontal TV lines

● No burn in, no image lag, no geometric distortion .

● Magnetically shielded .

● Composite video out at 1 Vpp @ 75 ohms .

● Power consumption 3W @ 12VDC

● Internal configurations for gamma, shutter speed, and AGC on/manual .

● Operating temperature range of –20 to 55°C .

● 50 dB S/N ratio

● External sync 0 .5 Vpp or composite input

● Includes standard tripod mount, power supply and 6-ft (~2m) BNC cable and RCA adaptor .

Color Video Camera for MicroscopyCOLCAMSpecifically chosen for its high quality video imaging and large 1⁄2” CCD (8mm), the Colcam is the clear choice for WPI Trinocular microscopes . This camera was specifically detailed for use with WPI standard C-mount couplers to assure the quality capture of still images and video . COLCAM incorporates the latest CCD image sensor for color signal output to “NTSC” (North American) or “PAL” (European) video systems .

A high signal-to-noise ratio of 50 dB permits clear images with

low light levels . A C-mount is required for use with microscopes . Includes a

standard tripod mount, power supply and 6-ft BNC-terminated cable with an RCA adaptor . (Lens not included.)

VCAMBW monochrome camera is available for either EIA (North American) or CCIR (European) video systems . Resolution is 600 horizontal TV lines . High 50 dB signal-to-noise ratio permits clear images with low light levels . Includes, power supply and 6-ft BNC-terminated cable with an RCA adaptor and has excellent IR response

Monochrome Video Camera for Microscopy 1.0

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0.0400 500 600 700 800 900 1000

Wavelength (nm)

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Microscope Mount: Please specify model of micro-scope when requesting a quote.

If PAL is needed in 120V line systems or NTSC in 240V mains systems, please specify.

Microscope Mount: Please specify model of microscope when requesting a quote.

If PAL is needed in 120V line systems or NTSC in 240V mains systems, please specify.

COLCAM SPECIFICAtIOnS VCAM (b&w) SPECIFICAtIOnSntSC PAL EIA CCIR

Image Device 1/2-inch CCD 1/2-inch CCD 1/2-inch CCD 1/2-inch CCD

Picture Elements 811 x 508 795 x 596 811 x 508 795 x 596

Scanning System 525 lines, 60 fields / sec 625 lines, 50 fields / sec 525 lines, 60 fields / sec 625 lines, 50 fields / sec

Minimum Illumination 0 .2 Lux/F1 .2 (5600°K) 0 .02 Lux -6 dB output acceptable illumination 0 .01 Lux

Resolution 470 TV lines 600 TV lines

Dimensions 35 x 35 x 42 mm (1 .4" x 1 .4" x 1 .7") 42 x 48 x 95 mm (1 .7" x 1 .9" x 3 .7"), excluding connectors

Weight < 300 g 390 g

See COLCAM mounted on PZMIII microscope, page 212.



COLCAMntSC Color CCD Video Camera (120 V, US plug) COLCAMPAL‑B Color CCD Video Camera (240 V, British plug) COLCAMPAL‑Z Color CCD Video Camera (240 V, Euro plug)

VCAMBWntSC B&W CCD Video Camera (120 V, US plug) VCAMBWPAL‑B B&W CCD Video Camera (240 V, British plug) VCAMBWPAL‑Z B&W CCD Video Camera (240 V, Euro plug)

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Peristaltic Pumps

Peri-StarTM Pro Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .224

MityFlex Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .226

Mini-Star™ Miniature DC Meristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .226

Vacuum Pump

LV140 Vacuum Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .227

Laboratory Syringe Pumps

Aladdin Syringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .227

SP Series Syringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 228

Eagle Pipetters

Eagle Pipetters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234

Universal Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235

Remote Micro Syringe Pump / Stereotaxic Injection

UltraMicroPump II Microsyringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232

DMP & MMP Manual Microsyringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239

Oocyte Injection

Nanoliter 2000 Microprocessor Controlled Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 238

Single and Multi-channel Micro Pressure Injection

PV820 Pneumatic PicoPump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236

PV830 Pneumatic PicoPump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236

Supplies & Tools

Syringes for SP Series and Aladdin Series . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230

Syringes, Microvolume, for UltraMicroPump II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233

Pressure Manometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239

NanoFil™ Specialty Microsyringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240

µTip™ Micropipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .242

Micro Cannula . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .242

MicroFil™ Non-metallic Syringe Needles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243

Pumps & Fluid Handling





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WPI carries a wide variety of fluid handling tools utilizing three different technologies: peristaltic pumps, syringe pumps and pneumatic injectors . The range of applications and the criteria by which one selects a particular pump or injector is broad and complex and we

encourage you to phone or email us and speak to a tech support specialist . In the meantime, we have tried to simplify the process by listing all of the pumps and injectors in a chart that sorts them by technology, flow range and special features . Special features tell you about

applications of the pump or technical aspects that are more properly discussed in a technical manual than a catalog entry . We frequently use the terms Infuse/Withdraw (I/W), Push/Pull and RS232, and these terms are defined below . Many of the syringe pumps can be had with an alarm option that alerts the user to end of travel or blocked flow and a “P” option that allows programmable flow and volume profiles without using the RS232 computer interface .

Infuse/Withdraw: Describes a feature of a syringe pump whereby the syringe plunger is held by the pusher so the motor can pull the plunger back, withdrawing fluid as well as pushing the plunger to infuse it .

Push/ Pull: Again, a feature of syringe pumps which use two syringes mounted back to back so movement of the pusher block will simultaneously withdraw fluid into one syringe and infuse an equal amount from the other .

RS232: This is a serial interface . Some pumps in the WPI line can be addressed by computer . Simple programs can be created to control the pump rate, the overall volume . Flow ramps or other profiles and temporal sequences can be written . In addition, pumps can be daisy-chained together to create a pump network . In such a network, each pump has its own address and can be addressed separately by the program .

Which pump is right for you?



Pump Type Fluid RangeNumber of Channels

Special features

Peripro-2H Peristaltic 0 .8 - 300 mL/min 2 Calibrated output Replaceable tubing cartridges

Peripro-4H Peristaltic 0 .8 - 300 mL/min 4 Calibrated output Replaceable tubing cartridges

Peripro-4L Peristaltic 0 .01-80 mL/min 4 Calibrated output Replaceable tubing cartridges

Peripro-8L Peristaltic 0 .01-80 mL/min 8 Calibrated output Replaceable tubing cartridges

MityFlex Peristaltic 1 .7-800 mL/min 1 High Volume

MINISTAR Peristaltic 0 .006- 37 mL/min 1 Compact design, Remote control

AL1000 Syringe 0 .000073-1699 mL/hr 1 Push/pull

AL2000 Syringe 0 .000073-1699 mL/hr 2 Push/pull (2 networked pumps)

SP100i Syringe 0 .0001-519 mL/hr 1 Basic 1 Channel

SP101i Syringe 0 .001 µL/hr – 35mL/min 2 Micro Dialysis application

SP200i Syringe 0 .001µL/hr - 145 mL/min 2 RS232 TTL/Footswitch

SP220i Syringe 0 .001µL/hr - 21 mL/min 10 RS232 Infuse Only

SP250i Syringe 0 .001µL/hr - 21 mL/min 4 RS232 Infuse Only

SP210iw Syringe 0 .001µL/hr - 145 mL/min 2 RS232 Infuse/Withdraw

SP230iw Syringe 0 .001µL/hr - 21 mL/min 10 RS232 Infuse/Withdraw

SP120p Syringe 0 .1 µL/hr – 127 mL/hr 1+1 push pull, single cycle

SP260p Syringe 0 .001µL/hr - 86 mL/min 2+2 RS232 push pull, single cycle

SP210c Syringe 0 .001µL/hr - 86 mL/min 2+2 RS232 push pull, continuous

UMP2 Syringe 0 .03nL/min - 10 µL/sec 1 Ultra Micro Infuse/Withdraw RS232

B203XV Syringe Bolus, 2 .3-69 mL/Injection 1 Oocyte Injector, Infuse only

MMP Syringe Manual 100 µL-1mL syringe 1 Manual

DMP Syringe Manual 100 µL-1mL syringe 1 Digital readout Micrometer

PV820 PneumaticBolus/Continuous Pressure,

Duration and Pipette dependent1 Injection Pressure and Holding Pressure

PV830 PneumaticBolus/Continuous Pressure,

Duration and Pipette dependent1 Injection Pressure and Holding Pressure and Vacuum

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High performance digital peristaltic pump at an affordable price!

Peri-Star™ Pro

Peri-Star™ Pro peristaltic pumps provide accurate and precise pumping with convenience and versatility . Peri-Star Pro can be run in either flow rate mode (mL/min) or rotation speed mode (rpm) . For good laboratory practice, pumps must be calibrated after changing the tubing and solution . Users can easily calibrate Peri-Star Pro to deliver flow as accurate as 0 .5% in a wide flow range from 0 .01 mL/min to 280 mL/min . Under rotation speed mode, the digitally controlled stepping motor provides accurate and reproducible operation with 0 .1% rpm both forward and in reverse .

Large backlit digital LCD display provides readouts of rotation direction, flow rate or rotation speed, tubing ID, drive status and

remote control mode simultaneously . Water resistant membrane keypad allows easy programming while protecting LCD display and controls from fluid entry .

Built-in Human Machine Interface (HMI) with screen instructions in plain English steps users through initial setup, calibration and operating procedures . The user-friendly interface reduces the need to frequently check the printed manual for instruction and reference .

Peri-Star Pro is available in two versions: a

4-roller version for high flow and an 8-roller version for lower volumes while providing high pressure with minimal pulsations .

A unique planetary gear design with eight actively driven rollers (four rollers for higher flow rate model), together with independent tubing compression fine adjustment, greatly increases flow accuracy and prolongs tubing life . Snap-on cartridges allow tubing to be changed quickly without cross contamination of solutions .

l Display either rotation speed (RPM) or flow rate (mL/min)

l Wide flow range: 0.01 - 280 mL/min

l Accuracy of flow rate: 0.5% using self calibration function

l Accuracy of speed: 0.1 rpm

l Large backlit digital LCD display

l Programmable for all tubing sizes between 0.8 mm and 6.4 mm ID

l Easy and fast tubing replacement using snap-on cartridges

l Membrane keypad allows easy programming while protecting controls from fluid entry

l Actively driven rollers by planetary gears for long lasting tubing life.





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PERI-STAR PRO SPECIFICATIONSPeri-Star Pro 2H / 4H

(High Rate)Peri-Star Pro 4L / 8L

(Low Rate)

Number of rollers 4 8

Number of chaNNels 2–4 4–8

rotor speed raNge 1–100 rpm 1–100 rpm

fluid flow raNge0.8–280 ml/min

#17 tubing: 3.5–280 ml/min0.01–80 ml/min

#14 tubing: 0.2–18 ml/min

tubiNg raNge 3.1–6.4 mm id 0.5–2.4 mm id

self-calibratioN Yes Yes

workiNg eNviroNmeNt 0–45°c, humidity < 80% 0–45°c, humidity < 80%

power 110 v or 220 v ac, 50 - 60 hz 110 v or 220 v ac, 50 - 60 hz

dimeNsioNs 190 x 162 x 275 mm 190 x 162 x 275 mm

shippiNg weight 11 lb / 5 kg 11 lb / 5 kg

Available in 2-, 4- and 8-channel versions

Pump head for PERIPRO-2H and PERIPRO-2H-220 (2 channels, 4 rollers)

Pump head for PERIPRO-4L and PERIPRO-4L-220 (4 channels, 8 rollers)

Pump head for PERIPRO-4H and PERIPRO-4H-220 (4 channels, 4 rollers)

Pump head for PERIPRO-8L and PERIPRO-8L-220 (8 channels, 8 rollers)



NEW PERI-STAR PRO PUMPSPERIPRO-2H peri-startm pro, 2-channel, high rate, large tubing PERIPRO-2H-220 peri-startm pro, 2-channel, high rate, large tubing (220v) PERIPRO-4H peri-startm pro, 4-channel, high rate, large tubing PERIPRO-4H-220 peri-startm pro, 4-channel, high rate, large tubing (220v) PERIPRO-4L peri-startm pro, 4-channel, low rate, small tubing PERIPRO-4L-220 peri-startm pro, 4-channel, low rate, small tubing (220v) PERIPRO-8L peri-startm pro, 8-channel, low rate, small tubing PERIPRO-8L-220 peri-startm pro, 8-channel, low rate, small tubing (220v)

OPTIONAL ACCESSORIES503049 replacement tubing cartridge, large503050 replacement tubing cartridge, small503022 replacement silicone tubing, 1m, 1.6 mm i.d., #14, with stops503023 replacement –silicone tubing, 1m, 6.4 mm i.d., #17503120 ttl control module

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MityFlexPeristaltic Pump

The MityFlex is an economical, variable speed, peristaltic pump that can handle a wide variety of viscosities from air to gases to heavy slurries, with consistent displacement .

This pump can be used for fluid transfer or for metering applications . By varying the tubing a wide range of flow rates can be achieved . This is a great value for medium to high flow applications .

MITYFLEX SPECIFICATIONSNUMBER OF ROLLERS 2

FLOW RATE 1 .7 to 800 mL/min . Variable speed-reversable

RPM RANgE 8-228

FLOW RATES Tubing I .D . Flow Rate Masterflex Equiv .

1/16 in . 1 .7-48 mL/min* #14 1/8 in . 7-190 mL/min #16 3/16 in . 16-456 mL/min #25 1/4 in . 28-800 mL/min #17

POWER 115 VAC / 60 Hz or 220 VAC / 50 Hz

DIMENSIONS 22 .6 x 14 .2 x 19 cm (8 .9 x 5 .6 x 7 .5 in .)

SHIPPINg WEIgHT 3 .6 kg (8 lb)

* To use 1/8 -in. or 1/16 -in. tubing, MITY-KIT is required.

Included with the MityFlex are four short lengths of Norprene™ tubing to fit into the roller mechanism — 1/16", 1/8", 3/16", 1/4" — and black rollers for use with 3/16" to 1/4" tub-ing . The optional MITY-KIT includes replacement tubing and a set of red rollers for use with 1/16" and 1/8" tubing . Norprene is a trademark of Saint-Gobain Performance Plastics Corp.

Mini★Star™

Miniature DC Peristaltic PumpThis compact and lightweight peristaltic pump fits just about anywhere . It can be mounted directly on the bench, in a regular rack or to a post . The MiniStar’s speed can be adjusted from 1rpm to 50 rpm . With recommended silicone tubing, the volume can be set from 0 .006 mL/min to 37 mL/min . The MiniStar also features a hand held remote control that allows users to start and stop the pump, purge or adjust its speed and direction .

MINISTAR SPECIFICATIONSCHANNEL 1

SPEED 1-50 .0 rpm, forward/reverse

FLOW RANgE 0 .006~37 mL/min

RESOLUTION 1 rpm (0 .1 rpm computer control)

SPEED CONTROL Remote control

DISPLAY Indicators for status and speed

POWER 12 V DC (110/220 VAC adapter incl .)

WORKINg CONDITION Temperature 0-40°C, humidity < 80%

TUBINg Two-stop Silicone Wall Thickness 0 .8~1 .0 mm Outer Diameter ≤ 4 .8mm

DIMENSION OF DRIVER 135×72×72 mm (L×W×H)

DIMENSION OF REMOTE CONTROL 105×50×16 mm (L×W×H)

WEIgHT OF DRIVER 0 .5 Kg



500282 MityFlex Peristaltic Pump (110 V) 500283 MityFlex Peristaltic Pump (220 V) MITY-KIT Replacement Parts Kit with Rollers

MITY-KIT includes 4 short lengths of Norprene™ tubing to fit into the roller mechanism — 1/16", 1/8", 3/16", 1/4" — and red rollers for use with 1/16" tubing.

MINISTAR Miniature Peristaltic Pump, 1-channel503120 TTL Control Module503121 silicone tubing w stops, 2.4mm id x 0.8mm wall x 1 m (5-pk) 503122 silicone tubing w stops, 1mm id x 1mm wall x 1 m (5-pk)



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The quietest, most compactVacuum Pump

LV140 SPECIFICATIONSvacuum -53.3 kpa (-15.75 in. hg)

rated pressure —

rated airflow 2.5 l/min (0.088 cfm)

maXimum pressure —

life eXpectaNcY 3000 hours or more

Noise level 40 db(a) or less

outlet micro coupler socket

iNlet micro coupler socket

dutY cYcle 60 minutes

dimeNsioNs 145 x 106 x 134 mm

weight 2.3 kg

LV140-Y

These linear-motor-driven free piston air compressors and vacuum pumps are designed with many technological breakthroughs . The compressors and pumps are compact, ultra-quiet and vibration-free . They feature a quick start-off response, small output air pulsation and low power consumption . In addition, these also have a long working life of up to 3000 hours and can be easily maintained . They are perfect as either pressure or vacuum sources for a wide range of applications, including filtration, bubbling, and pneumatic driving . The LV140 vacuum pump is suitable for WPI’s 900A Micropressure System and PV800 series pumps .

Aladdinl Programmable

l Economical

l Versatile

l More Features

l Dual Pumping Action

The Aladdin was developed after years of design experience responding to researchers’ requests on what an Infusion/Withdrawal pump should be .

ALADDIN SPECIFICATIONSSYRINgE SIZES 1-60 mL, or 0 .5-5 µL micro syringes

NUMBER OF SYRINgES 1, 6, or 8, depending on model

MOTOR TYPE Step Motor, 1/8 to 1/2 step modes

STEPS PER REVOLUTIONS 400

STEPPINg (max . min .) 0 .21 µm to 0 .850 µm

MOTOR TO DRIVE SCREW RATIO 15/28

SPEED (max ./min .) 5 .1 cm/min / 0 .0042 cm/hr

PUMPINg RATES 1699 mL/hr with 60 mL syringe, to

0 .73 µL/hr with 1 mL syringe

MAXIMUM FORCE 35 lb at min . speed, 18 lb at max . speed

NUMBER OF PROgRAM PHASES 41

RS-232 PUMP NETWORK 100 pumps maximum

POWER SUPPLY Wall adapter 12V DC @ 850 mA

DIMENSIONS 22 .9 x 14 .6 x 11 .4 cm

(8 .75 x 5 .75 x 4 .5 in .)

WEIgHT 1 .6 kg (3 .6 lb)

Need a pump for two syringes? Two Aladdin pumps when daisy-chained are more efficient and affordable than any competitor’s dual syringe models . Two Aladdins (AL-2000) will perform as a dual infusion/withdrawal pump, a double pump for infusing at different rates, a push/pull pump with one infusing and one withdrawing at the same or different rates, two independent pumps, or a master/slave pump . One Aladdin can even control the second for continuous pumping with optional check valve set .

The Aladdin pump series will accept syringes from Becton Dickinson, Monoject, Terumo, and Air-Tite .

Programmable Syringe Pump



LV140-Y Vacuum Pump (120 V) Includes one fitting for 3/16-in. (4.75 mm) ID tubing.

(220V-240V version no longer available .)

AL-1000 Programmable Syringe Pump AL-2000 Two AL1000 Syringe Pumps

Includes GN-TTL Interconnecting Cable for push/pull or continuous pumping. Valves not included.

Specify line voltageWhen ordering 220V models, specify UK, Euro or Australian line cord.

OPTIONAL ACCESSORIESGN-PC7 PC to pump cable, 7 ftGN-PC25 PC to pump cable, 25 ftGN-NET7 Pump-to-pump network cable, 7 ftGN-NET25 Pump-to-pump network cable, 25 ft GN-TTL Pump-to-pump reciprocating cableADPT2 Footswitch

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Syringe PumpsMicroprocessor-controlled syringe pumps for applications requiring high metering precision at low, pulse-free rates

Sturdy and reliable, extremely simple to set up and use — and surprisingly affordable . Liquid crystal displays (LCDs) prompt you through setup:

1. Select syringe from table stored in the pump’s memory and displayed on the LCD .

2. Enter the volume to be dispensed .

3. Enter the flow rate, press “start .”

It’s fast and simple . Your settings are permanently stored in memory — there’s no need to re-enter them each day . SP pumps feature preset rate and volume control . Just set the volume you want dispensed . Volume is tracked continuously on the LCD display . Then, when the preset volume has been dispensed, the pump shuts off automatically . The easy-to-read digital display provides real-time readings using both parameters and values for clearer, mistake-free readings . The SP200 Series pumps offer TTL and RS-232C interfaces and automatic shutoff under stall conditions .

SP100i SPECIFICATIONS

Mode infusion

Syringe Size 10 µl to 60 ml (one)

Maximum Flow Rate 519 ml/hr

(60 ml syringe)

Minimum Flow Rate 0.1µl/hr

(10 µl syringe)

Linear Force 20 ib (9 kg)

Advance Per Microstep 0.529 micron (1/2-step)

Maximum Step Rate 400 steps/sec

(1/2-step)

Minimum Step Rate 1 step/30 sec

Accuracy < 1% error

Reproducibility ± 0.1%

Dimensions 9 x 6 x 5.5 in.

23 x 15 x 14 cm

Shipping Weight 7.5 ib (3.4 kg)

Available 95-135 V or 220-240 V, 50/60 Hz

Single-Syringe Infusion PumpSP100iThis inexpensive single-syringe infusion pump combines precision and simplicity with outstanding ease of use and durability .

l Holds any size syringe, 10 µL to 50 mL

l Automatic volume control and shutoff

l Simple menu-driven setup: dispense volume, dispense flow rate, syringe diameter (selected from displayed table)

l Last settings stored in permanent memory

l Continuous dispense volume display

SPECIFICATIONS

SP101i

Mode infusion (microdialysis)

Syringe Size 10 µl to 10 ml (two)

Maximum Flow Rate 0.351 ml/min. (10 ml syringe)

Minimum Flow Rate 0.001µl/min (10 µl syringe)


Advance Per Microstep 0.088 micron (1/2-step)

Maximum Step Rate 400 steps/sec (1/2-step)


Accuracy < 1% error


Dimensions 9 x 6 x 5.5 in. 23 x 15 x 14 cm



Developed especially for use in microdialysis experiments, these pumps produce very fine syringe movement . The modified SP100i gearing features a sixfold gear reduction compared to standard models, allowing pumping at much smaller flow rates . (See sensors such as ISO-NO and Micro-C for detection of dialysates.) The SP101i is a dual barrel infusion only pump .

l Holds two syringes, 10 µL to 10 mL

Two-Syringe Microdialysis PumpSP101i



SP100i syringe pump, infusion (single), 95-135 v SP100iZ syringe pump, infusion (single), 220-240 v SP101i syringe pump, microdialysis (double, slow speed), 95-135 v SP101iZ syringe pump, microdialysis (double, slow speed), 220-240 v

All 240-volt pumps are CE-approved.

####-A audible alarm (add “a” to end of pump part number when ordering)



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This feature-laden two-syringe infusion pump combines a broad speed range and holds a wide range of syringe sizes to meet the requirements of virtually any laboratory application .

l Holds two syringes, 10 to 140 mL

l Backlit LCD display

l Knob locks/unlocks drive block for effortless, drag-free adjustment

l Simple menu-driven setup: Syringe diameter (selected from displayed table), Dispense volume, Dispense flow rate


l Preset volume control and automatic shutoff

l Settings can be reviewed or changed during operation

l Optical encoder stall detection

l Choice of unit selection


l Built-in RS-232C interface for computer linking or

Two-Syringe Infusion PumpSP200i

SP200i SPECIFICATIONS SP200i

Mode infusion


Maximum Flow Rate 145 ml/min (140 ml syringe)



Advance Per Microstep 0.165 micron



Accuracy < 1% error



Shipping Weight 12 ib (5.4 kg)



Mode infusion

Syringe Size 10 µl-10 ml (four) (syringes may be different sizes)

Maximum Flow Rate 20.91 ml/min (10 ml syringe)






Accuracy < 1% error


Dimensions 11 x 9 x 6 in. 28 x 23 x 15 cm



Four-Syringe Nanoliter Infusion PumpSP250i

Multi-Syringe Infusion PumpSP220i


Mode infusion

Syringe Size 10 µl to 10 ml (ten) 10 µl to 60 ml (six) 10 µl to 140 ml (four)*


Minimum Flow Rate 0.001µl/hr (10 µl syringe )

Linear Force 40 lb (18 kg)




Accuracy < 1% error





Ideal for applications requiring multiple syringes, this pump has been modified to hold up to hold:

l One to ten syringes up to 10 mL

l One to six syringes, 20 mL to 60 mL

l One to four syringes, 100 mL to 140 mL .

Each syringe can be sized differently and is clamped independently

l Holds four syringes, up to 10 mL each .

l Separate clamping accommodates different sizes .

l Syringes may be positioned independently for sequential dispensing by the pusher block .



SP200i Syringe Pump, Infusion (double), 95-135 V SP200iZ Syringe Pump, Infusion (double), 220-240 V SP220i Syringe Pump, Infusion (multiple), 95-135 V SP220iZ Syringe Pump, Infusion (multiple), 220-240 V SP250i Syringe Pump, Infusion (multiple, mixed volumes), 95-135 V SP250iZ Syringe Pump, Infusion (multiple, mixed volumes), 220-240 V


####-A Audible Alarm (add “A” to end of pump part number when ordering) ####-P programmable ramp option (sp200 series) (add “p” to end of pump part number when ordering)

OPTIONAL CABLES15623 Serial cable, SP Pump-to-IBM 9-pin “D” connector15624 Serial cable, SP Pump-to-Macintosh connector15676 Serial cable, SP Pump-to-IBM 25-pin “D” connector13685 SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft13962 Footswitch for SP200 Series Pumps

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SP210iw SPECIFICATIONSMode infusion/withdrawal

Syringe Size 10 µl to 140 ml (two)*







Accuracy < 1% error


Dimensions 11 x 9 x 5.5 in.

28 x 23 x 14 cm



Two-Syringe Infusion/Withdrawal PumpSP210iwThe SP210iw offers you more advanced features than any other infusion/withdrawal pump in its price range—including five operating modes plus independent rate and volume settings for both infusion and withdrawal .

l All features of SP200i

l Independent rate and volume settings for infusion and withdrawal

l Multiple mode selection: infusion, withdrawal, infusion then withdrawal, withdrawal then infusion, or continuous cycle .

SP230iw SPECIFICATIONS SP230iw

Mode infusion/withdrawal

Syringe Size 10 µl to 10 ml (ten) 10 µl to 60 ml (six) 10 µl to 140 ml (four)*


Minimum Flow Rate 0.001µl/hr (10 µl syringe )





Accuracy < 1% error





Multi-Syringe Infusion/Withdrawal PumpSP230iwIdeal for applications requiring multiple syringes, the SP220i and SP230iw are adaptations of the SP200i and SP210iw, respectively . Each has been modified to hold up to 10 syringes .

l SP220i has all features of SP200i

l SP230iw has all features of SP210iw

l Both have the same multiple syringe holder accommodating 10 syringes up to 10 mL, or six syringes up to 50 mL, or four syringes up to 140 mL .



Syringes with Luer Fitting (no needle) WPI Part # Volume Description O.D. ILS005LT 5 µL ILS 5 µL gas-tight Luer tip 6 .5 mmILS010LT 10 µL ILS 10 µL gas-tight Luer-tip 6 .5 mmILS025TLL 25 µL ILS 25 µL gas-tight Luer tip* 8 .0 mmILS050TLL 50 µL ILS 50 µL gas-tight Teflon Luer Lock* 8 .0 mmILS100TLL 100 µL ILS 100 µL gas-tight Teflon Luer Lock* 8 .0 mmILS250TLL 250 µL ILS 250 µL gas-tight Teflon Luer Lock* 8 .0 mmILS500TLL 500 µL ILS 500 µL gas-tight Teflon Luer Lock* 8 .0 mm300035 6/32 stability button for SP200 Series pumps *Has 6/32 button thread. The 6/32 stability button allows the SP series pumps to drive the syringe plunger in a precise direction to minimize variations in the syringe body/plunger offset.

ILS is a trademark of Innovative Labor Systeme.

SP210iw syringe pump, infusion & withdrawal (double), 95-135 v SP210iwZ syringe pump, infusion & withdrawal (double), 220-240 v SP230iw syringe pump, infusion & withdrawal (multiple), 95-135 v SP230iwZ syringe pump, infusion & withdrawal (multiple), 220-240 v


####-A audible alarm (add “a” to end of pump part number when ordering) ####-P programmable ramp option (sp200 series) (add “p” to end of pump part number when ordering)

OPTIONAL CABLES15623 Serial cable, SP Pump-to-IBM 9-pin “D” connector15624 Serial cable, SP Pump-to-Macintosh connector15676 Serial cable, SP Pump-to-IBM 25-pin “D” connector13685 SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft13962 Footswitch for SP200 Series Pumps



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SP260p & SP210cSPECIFICATIONS

Mode sp260p push-pull (single cycle) sp210c infusion/withdrawal (continuous)

Syringe Size

sp260p 10 µl-60 ml (four) sp210c 10 µl-60 ml (four)

Maximum Flow Rate

sp260p 86 ml/min (60 ml syringe) sp210c 86 ml/min (60 ml syringe)






Accuracy < 1% error





A second syringe mount has been added to the basic SP100i, with both syringes activated by a single pusher block for simultaneous infusion and withdrawal . This pump has all the features of the SP100i, plus:

l Holds two syringes, from 10 µL to 10 mL .

Two-Syringe Push-Pull PumpSP120p

SP120p SPECIFICATIONSMode infusion/withdrawal


Maximum Flow Rate 125 ml/hr (10 ml syringe)

Minimum Flow Rate 0.1 µl/hr (10 µl syringe )





Accuracy < 1% error





Continuous Cycle Syringe PumpSP210c

Four-Syringe Push-Pull PumpSP260p single cycleThe SP260p can hold up to four syringes and can cycle continuously back and forth in a push-pull action . As two syringes are infusing, two other syringes are withdrawing at the same rate . The SP260p is used for single-cycle applications only . With the use of 3-way valves the pump can empty and refill syringes for a continuous dispense .

l Knob locks/unlocks drive block for effortless, drag-free adjustment

l Simple menu-driven setup: Syringe diameter (selected from displayed table), Dispense volume, Dispense flow rate


l Preset volume control and automatic shutoff

l Settings can be reviewed or changed during operation

l Optical encoder stall detection

l Choice of unit selection


l Built-in RS-232C interface for computer linking or “daisy chaining” up to 100 pumps .

l TTL interface for foot switch, timer, relay control; outputs for run indicator, valve control

action . As two syringes are infusing, two other syringes are withdrawing at the same rate . At the end of the set volume the direction is automatically reversed and the next cycle begins . With the use of 3-way valves, the pump can empty and refill syringes for a continuous dispense .

l Holds four syringes, 10 mL to 60 mL each . With larger syringes the full volume may not be usable . [With a 60 mL syring, 40 mL is usable; with a 30 mL syringe, the full volume is usable .]

The SP210c can hold up to four syringes and can cycle continuously back and forth in a push-pull



SP120p Syringe Pump, Infusion-Withdrawal (double), 95-135 V SP120pZ Syringe Pump, Infusion-Withdrawal (double), 220-240 V SP260p Syringe Pump, Infusion-Withdrawal (double) Single Cycle Action, 95-135 V SP260pZ Syringe Pump, Infusion-Withdrawal (double) Single Cycle Action, 220-240 V SP210c Syringe Pump, Infusion & Withdrawl (Continuous Action), 95-135 V SP210cZ Syringe Pump, Infusion & Withdrawl (Continuous Action), 220-240 V


####-A Audible Alarm (add “A” to end of pump part number when ordering) ####-P programmable ramp option (sp200 series) (add “p” to end of pump part number when ordering)

OPTIONAL CABLES15623 Serial cable, SP Pump-to-IBM 9-pin “D” connector15624 Serial cable, SP Pump-to-Macintosh connector15676 Serial cable, SP Pump-to-IBM 25-pin “D” connector13685 SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft 13962 Footswitch for SP200 Series Pumps

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See FlexiFil™ — the recommended

microsyringe for in vivo injection

See FlexiFil™ — the recommended

microsyringe for in vivo injection

Enclosed lead screw

More stable tip during infusion

Greater plunger

travelSyringe holder

holds wider variety of

syringes (5.5 to 9 mm)

Perfect for a wide range of applications including intracellular injection, micro delivery of biochemical agents or dyes, cell separation, and in vitro fertilization.

Micro syringes are easily installed — just snap the barrel into the clamps. UMP2 accepts a range of syringes from 0.5 µL to 1 mL.

See “Reproducible and Efficient Murine CNS Gene Delivery Using a Microprocessor Controlled Injector,” A.I. Brooks et al., Journal of Neuroscience Methods, 80 (1998) 137-147.

UMP2 SPECIFICATIONS(based on 10 µL syringe)

TOTAL # OF STEPS 20,000 (63 mm travel)

MINIMUM DISPENSINg VOLUME 0 .58 nL / step

LINEAR MOTION PER STEP 3 .175 microns

WEIgHT 325 g (11 .5 oz)

MOUNTINg ROD DIAMETERS 7 .9 mm (0 .31 in .)CONTROLLER POWER REQUIREMENTS 2 amps, 12 V DC

DIMENSIONS ø 32 mm x 190 mm (ø 1 .3 in . x 7 .5 in .)

Man

ipul

ator

not

incl

uded

.This versatile injector uses microsyringes to deliver picoliter volumes

Enclosed lead screw

More stable tip during infusion

Greater plunger

travelSyringe holder

holds wider variety of

syringes (5.5 to 9 mm)

UltraMicroPump II





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UltraMicroPump II

Microvolume Syringes

* The plunger extends to the tip of the needle, displacing the full sample during injection - which gives the syringe zero dead volume . The barrel length of this syringe is 17 cm long vs . the usual 8-9 cm .SgE and ILS are respective trademarks of Scientific glass Engineering and Innovative Labor Systeme .

Smart ControllerAn Integral component in the UMP2 system is a microprocessor-

based controller, Micro4, which provides an “intelligent” and easy-to-use interface to up to four syringe pumps . Operating parameters are set with the membrane keypad and LCD display . From the keypad the user can select the following functions: set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, rate of delivery, and syringe type as well as synchronize the starting and stopping of any combination of syringe pumps .

User parameters can be stored in the device’s “non-volatile” memory for instant recall when the unit is powered on .

An optional footswitch can be plugged into a connector on the rear of the controller for “hands free” start-/-stop operation .

Computer Control—An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs .

UMPII shown mounted to stereotaxic frame (not included).

With its digital controller, UltraMicroPump II can dispense as little as 600 picoliters per incremental advance of the syringe piston (using a 5-µL syringe) . Syringes may be filled externally and then inserted into the pump or filled while mounted in the pump . Fluids injected or withdrawn are held entirely within the micro syringe to maintain a low fluid dead volume .

For positioning, the UltraMicroPump II may be attached to any of several WPI micro-positioners such as the M3301 (manual), DC3001 (motorized), or any manual stereotaxic manipulator .

The world’s leading ultramicropump!



The recommended microsyringe for use with UMPII is WPI’s FlexiFil™

Syringes with Luer Fitting (no needle)WPI P/N Volume Description O.D. UMP2 UMP1ILS005LT 5 µL ILS 5 µL gas-tight Luer tip 6 .5 mm Y N ILS010LT 10 µL ILS 10 µL gas-tight Luer tip 6 .5 mm Y N ILS025LT 25 µL ILS 25 µL gas-tight Luer tip 8 .0 mm Y N SGE050TLL 50 µL SgE 50 µL gas-tight Teflon Luer Lock 8 .0 mm Y Y SGE100TLL 100 µL SgE 100 µL gas-tight Teflon Luer Lock 8 .0 mm Y Y SGE250TLL 250 µL SgE 250 µL gas-tight Teflon Luer Lock 8 .0 mm N N

Syringes with Replaceable Beveled NeedlesWPI P/N Volume Description O.D. UMP2 UMP1SGE0005RN* 0 .5 µL SgE 0 .5 µL 23 ga (0 .63 mm) 70 mm long 8 .0 mm Y Y SGE001RN* 1 .0 µL SgE 1 .0 µL 26 ga (0 .47 mm) 70 mm long 8 .0 mm Y Y SGE005RN 5 µL SgE 5 µL 23 ga (0 .63 mm) 50 mm long 8 .0 mm Y Y SGE010RNS 10 µL SgE 10 µL 26 ga (0 .47 mm) 50 mm long 8 .0 mm Y Y SGE025RN 25 µL SgE 25 µL 25 ga (0 .50 mm) 50 mm long 8 .0 mm Y Y SGE050RN 50 µL SgE 50 µL 25 ga (0 .50 mm) 50 mm long 8 .0 mm Y Y SGE100RN 100 µL SgE 100 µL 25 ga (0 .50 mm) 50 mm long 8 .0 mm Y Y

Replacement NeedlesRN0005 For syringe SgE0005RN, 23 ga (0 .63 mm) 70 mm longRN001 For syringe SgE001RN, 26 ga (0 .47 mm) 70 mm longRN005 For syringe SgE005RN, 23 ga (0 .63 mm) 50 mm longRN010 For syringe SgE010RN(S), 26 ga (0 .47 mm) 50 mm long, 5-packRN025 For syringes SgE025RN, SgE050RN, SgE0100RN, 26 ga (0 .47 mm) 50 mm long, 5-pack

UMPII ACCEPTS: glass syringes with barrel diameters from 5.5 to 9 mm.

UMP2-1 UltraMicroPump II (one) and Micro4 Controller UMP2-2 UltraMicroPump II (two) and Micro4 Controller UMP2-3 UltraMicroPump II (three) and Micro4 Controller UMP2-4 UltraMicroPump II (four) and Micro4 Controller UMP2 UltraMicroPump II (without controller) MICRO4 Micro4 Controller, Four-Channel

OPTIONS AND ACCESSORIES15867 Footswitch for Micro440500 RS-232 Cable, 9-pin “D” connector502201 V-clamp for Stereotaxic Frame

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Lifetime Warranty

Lasts 20 times Longer

Comfortable ergonomic design

Eagle™ Adjustable Pipetters

Replacement parts

Replacement parts for the Eagle Pipetters are available from our website at www.wpiinc.com or call us for details .

Built with ultra high molecular weight polyethylene (UHMWPE), the piston seal (patent pending) lasts at least 20 times longer than those made with PTFE. The pipetter delivers with the same accuracy and precision even after more than 51 million non-stop press-release strokes.

l Superior WPI seals give reliable and accurate experimental results.

l Calibration interval can be significantly increased.

l Priced so affordably that everyone in the lab can have a set.

l Customize your set by selecting only the pipetters you need.

l Lifetime warranty on the piston seal.



Model Volume Range Price

A E2 0 .2 - 2 .0 µL US$99

B E10 0 .5 - 10 µL US$99

C E20 2 - 2 .0 µL US$99

D E100 20 - 100 µL US$99

E E200 50 - 200 µL US$99

F E1000 100 - 1000 µL US$99

G E5000 1000 - 5000 µL US$99

STORAGE500876 6 pipetter rack stand US$35500877 pette-clamp, 3/pkg US$3514239 original pipetter stand US$79

Buy any set of 5 or 6and get one free!

sets es5, es6, and es7 include your selection of pipetters, along with a stand (#14239) for convenient storage. simply specify the pipetters you want by including the letter designation from the chart on the right. for example, es5-aabcd would include two e2s and one each e10, e20 and e100.

ES5 pipetters (set of any 5) and stand US$495ES6 pipetters (set of any 6) and stand US$575ES7 pipetters (set of any 7) and stand US$650500801 filters for e5000 (5-pack) US$15



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Universal Pipette TipsUltra-clear and certified RNase/DNase-free

ABCABCabcABCABCABCABCABCABCABCABCABC

WPI’s Universal Pipette Tips are for use with Eagle and most other pipetters, including Gilson, Oxford Benchmate, Socorex, and SealPette.

* Tips 500197 and 500198 fit Eagle, Eppendorf, and BioHit pipetters.

500191, 500192 500193, 500194 500195, 500196

500199 500200

500201

500197500198

500202

500203

500204

500205500206

500207

Same as leading brands

— at about half the price!



Universal TipsTip Volume For Pipetter Bulk Part No. Price Rack Part No. Price

0 .1 - 10 µLEagle E2Eagle E10Eagle E20

Bag of 1000 500191 US$15 960 (10 racks of 96) 500192 US$30

5 - 200 µLEagle E20Eagle E100Eagle E200

Bag of 1000 500193 US$15 960 (10 racks of 96) 500194 US$30

100 - 1000 µL Eagle E1000 Bag of 1000 500195 US$17 1000 (10 racks of 100) 500196 US$35

500 - 5000 µL Eagle E5000 Bag of 250 500197 * US$15 500 (10 racks of 50) 500198 * US$55

Universal Filter Tips (sterile)Tip Volume For Pipetter Bulk Part No. Price Rack Part No. Price

0 .1 - 10 µLEagle E2Eagle E10Eagle E20

960 (10 racks of 96) 500199 US$60

10 - 200 µLEagle E20Eagle E100Eagle E200

960 (10 racks of 96) 500200 US$65

100 - 1000 µL Eagle E1000 1000 (10 racks of 100) 500201 US$65

Universal Gel Loader Tips (flexible)Tip Volume For Pipetter Bulk Part No. Price Rack Part No. Price

0 .1 - 10 µL (Flat)0 .17 mm OD

Eagle E2Eagle E10Eagle E20

200 500202 US$40

0 .1 - 10 µL (Flat)0 .37 mm OD


200 500203 US$40

0 .1 - 10 µL (Round)

0 .57 mm OD


200 500204 US$20

0 .5 - 200 µL (Round)

0 .57 mm OD


Bag of 1000 500205 US$30 200 500206 US$20

0 .5 - 200 µL (Flat)

0 .37 mm OD


200 500207 US$40

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Pneumatic PicoPumpsRepeatable microinjection in volumes ranging from picoliters to nanoliters

and Hold pressure by a precision timing circuit that controls a solenoid valve . Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection . Vacuum is regulated the same way, by a 20-turn knob on the front panel . Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel . Vacuum can also be routed to the eject port .

New PicoNozzle Kit 5430-ALL (included) allows micropipettes to be securely mounted in micropositioners for stable axial air delivery. Because air enters the pipette axially, lateral whipping during injection is eliminated.

PV830 — Eject pressure, Hold pressure, and Vacuum are all available, controlled by separate regulators on the front panel . Eject pressure supplies a high-pressure pulse for injecting fluid . Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive . Pressure in the injection pipette is automatically switched between Eject

are adjusted independently by control knobs and indicator gauges on the front panel . Injection pressure is controlled by a 20-turn regulator on the front panel . A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port . Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting . The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse . A 5-volt monitor output provides a logic-level pulse for your computer or other monitoring device .

Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use carefully regulated air pres-sures for securing cells and injecting them with fluid . Injected volumes range from picoliters to nanoliters . Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suc-tion for supporting the cell or for filling the pipette from the tip . A second pressure port maintains a low positive “holding” pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion . Timing, ejection pressure, holding pressure, and suction

For a complete list of pre-pulled micropipettes, see µTips™, or call us with your special requirements.

The most recognized picopump in the world!

See NanoFil™ kits — page 240 .



Each PicoPump is supplied with two PicoNozzle Kits (5430-ALL) plus tubing to connect the holders to the pressure and vacuum ports.

PV820 PicoPump w/ hold pressure PV830 PicoPump w/ hold pressure and vacuum

Specify line voltage All PicoPumps require external vacuum source — see below.

OPTIONAL ACCESSORIES3260 Foot Switch2932 Rack Mount Kit, 31⁄2-in . high (PV820) 2933 Rack Mount Kit, 51⁄4-in . high (PV830) 5430-10 PicoNozzle Kit (MPH6S for 1 .0 mm pipette & 5-ft tubing assembly) 5430-12 PicoNozzle Kit (MPH6S for 1 .2 mm pipette & 5-ft tubing assembly) 5430-15 PicoNozzle Kit (MPH6S for 1 .5 mm pipette & 5-ft tubing assembly) 5430-20 PicoNozzle Kit (MPH6S for 2 .0 mm pipette & 5-ft tubing assembly)

5430-ALL picoNozzle kit (for 1.0, 1.2, 1.5, and 1.65 mm pipettes & 5-ft tubing assembly)

MPH6S Micropipette Holder (specify 1 .0, 1 .2, 1 .5 or 2 .0 mm) MPH6R Micropipette Holder (specify 1 .0, 1 .2, 1 .5 or 2 .0 mm) 3316 Replacement Input KitLV140-Y Vacuum Pump (120V)



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PV820 adds to the basic unit a regulated Hold pressure, used to maintain a low pressure in the pipette between injections to prevent unwanted fluid uptake by capillary action or diffusion . A precision timing circuit switches from Eject pressure to Hold pressure automatically, once

PICOPUMP SPECIFICATIONS PV820 PV830PRESSURE PRESSURE INPUT 0 to 150 psi 0 to 150 psi PRESSURE OUTPUT 0 .3 to 90 psi * 0 .3 to 90 psi LOWEST REgULATED PRESSURE 12 in . water * 12 in . water REgULATOR ACCURACY 0 .1% (20-turn dial) * 0 .1% (20-turn dial) * REgULATOR REPEATABILITY 0 .05 psi * 0 .05 psi * gAUgE ACCURACY 3% at full scale * 3% at full scale * INPUT CONNECTOR Quick Connect (1⁄4 in . OD Tubing) Quick Connect (1⁄4 in . OD Tubing) OUTPUT CONNECTOR Barbed (1⁄16 in . ID Tubing) Barbed (1/16 in . ID Tubing) CONTROL Solenoid Solenoid * Both Hold and Eject Pressures * Both Hold and Eject Pressures

VACUUM VACUUM INPUT 0 to 30 .0 in . Hg 0 to 30 .0 in . Hg VACUUM OUTPUT Unregulated 0 .2 to 29 .9 in . Hg LOWEST REgULATED VACUUM Unregulated 3 in . water REgULATOR ACCURACY Unregulated 0 .1% (20-turn dial) REgULATOR REPEATABILITY Unregulated 0 .03 in . Hg gAUgE ACCURACY None 3% at full scale INPUT CONNECTOR Quick Connect (1⁄4 in . OD Tubing) Quick Connect (1⁄4 in . OD Tubing) OUTPUT CONNECTOR Barbed (1⁄16 in . ID Tubing) Barbed (1⁄16 in . ID Tubing) CONTROL Manual Manual VENT Atmosphere Atmosphere

CONNECTIONS INCLUDED INPUT KIT 10 ft nylon tubing (0 .25” OD, 1000 psi), one 1⁄2” female NPT adapter OUTPUT KIT Two PicoNozzle assemblies, each consisting of one MPH6S pipette holder, 60-in . of PVC tubing (200 psi), and a luer-fitted aluminum handle .

PHYSICAL SPECIFICATIONS POWER 95-135 V or 220-240 V, 50/60 Hz 95-135 V or 220-240 V, 50/60 Hz DIMENSIONS 17 x 3 .5 x 9 .5 in . (43 x 9 x 24 cm) 17 x 5 .25 x 9 .5 in . (43 x 13 x 24 cm) SHIPPINg WEIgHT 11 lb (5 kg) 14 lb (6 .3 kg)

Application Example:Using PV830 for holding and injection of cells

Luer-to-Tubing Coupler Assortment Kits

—see page 172

Pressure Manometers—see page 239 .

timing has been set . Although regulated vacuum is not provided in this model, suction can be provided by connecting a vacuum source to the vacuum port on the rear panel . Suction is then available through the pressure ports .



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WPI’s microprocessor-controlled Nanoliter 2000 uses direct piston displacement . By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected . The process is quiet and vibration free . Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec) . Maximum fluid ejection is 5 µL . Each unit comes with sufficient glass to pull at least 300 tips . glass is 1 .14 mm O .D . (nominal) and 0 .5-mm I .D .

By setting the DIP switch, the injection volume can be changed

Nanoliter InjectorFor oocyte injection and applications in the 2 to 70 nanoliter range

NANOLITER 2000 SPECIFICATIONS

INJECTION VOLUME VariableREMOTE CONTROL YesgLASS OD 1 .14 mmgLASS ID 0 .5 mmSTEP 12 .7 µ/stepINJECTION SPEED Slow 23 nL/sec Fast 46 nL/secFILL SPEED Slow 23 nL/sec Fast 46 nL/secEMPTY SPEED 92 nL/secVARIABLE VOLUME RANgE 2 .3 - 69 .0 nLSMALLEST VOLUME 2 .3 nLTO CHANgE VOLUME Set switchINJECTIONS PER FILLINg, MAX . 100 injectionsSHIPPINg WEIgHT 3 lb . (1 .1 kg)

from 2 .3 to 69 .0 nL in 16 steps . Up to 100 injections may be triggered per filling . Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment .

Included: 1 vial 3 .5 in . capillaries (100); 1 vial 7 in . capil-laries (100); replacement “O” rings; Allen wrench; MicroFilTM MF34g backfilling needle; and two sample µTip™ pre-pulled micropi-pettes .

NANOLITER 2000NANOLITER 2000

Smart Controller: Micro4, an optional microprocessor-based controller, can provide an “intelligent” and easy-to-use interface to up to four Nanoliter Injectors . Operating parameters are set with the membrane keypad and LCD display . From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors . User parameters can be stored in the device’s “non-volatile” memory for instant recall when the unit is powered on . An optional footswitch can be plugged into a connector on the rear of the controller for “hands free” start/stop operation . An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs .

Micromanipulator not included.

Adapter #500778 (included free) extends the “reach” of a

manipulator-mounted injector.Optional smart controller

for Nanoliter 2000 (order as B203MC4)



WPI recommends the following equipment for a complete system,: PZMIII stereo microscope • MMJ joystick micromanipulator • M10 magnetic base • PUL-100 micropipette puller or µTip™ micropipettes .

B203XVY Nanoliter 2000 (120 V, U .S . plug) B203XVZ Nanoliter 2000 (240 V, Continental plug) B203XVB Nanoliter 2000 (240 V, British plug) B203MC4 Nanoliter Injector & Micro4 Controller (small controller not included)

OPTIONAL ACCESSORIES13142 Footswitch for Nanoliter 200015867 Footswitch for Micro4 Controller4878 Replacement 3 .5-in . glass capillaries (300) 4879 Replacement 7-in . glass capillaries (300) TIP10XV119 Micropipettes for Nanoliter Injector (10) MICRO4 Micro4 Controller, Four-Channel300033 Adapter for Micro45340 Spare Parts Kit (includes MicroFilTM MF34G, displacement piston, five O-ring sets) 500778 Replacement Nanoliter Injector Universal Adapter502859 Flared O-Ring Kit500299 Pistons, 5-pack503066 Flared glass, 100 pieces



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Manual Microsyringe Pump

The MMP and DMP are convenient tools for precise manual injection of fluid using glass pipettes or similar injection devices . The design allows visual feedback of flow at the pipette tip . They can also be used as a manual micro syringe pump for perfusion or withdrawal of liquids . The resolution of the injection volume can be continuously varied from 10 nanoliters to the microliter range, depending on the syringe used . Either oil or air can be used as the transfer media to assist the injection of fluid . The DMP comes with an exclusive digital micrometer that will allow the reading of piston advancement easily with a 0 .001-millimeter resolution . The optional software and cable kit can transmit advancement data directly into computer . Model MMP has the traditional mechanical micrometer head with a resolution of 10 microns per division and advances 500 micrometers per revolution . The entire frame body of the injector is constructed with polished

DMP & MMP SPECIFICATIONSTravel Distance 25 mm

Advances Resolution 0 .001 mm for DMP and 0 .01 mm for MMP

Syringe Size 10 µL to 1 mL gas tight luer tip syringe

Tubing 1 .5 m of PTFE tubing with 0 .5 mm ID

Pipette Holder 0 .24" x 5 .2"

Pipette Holder Fits 1 .0 to 1 .5 mm OD pipette

stainless steel for excellent stability and durability . The piston of the micrometer can be slid across the rail to the syringe's plunger position . Small diameter PTFE tubing is used to improve the accuracy and solution compatibility . The unique design of the pipette holder can securely hold any pipette with an outer diameter of between 1 .0 mm and 1 .5 mm . All necessary accessories for removing air and filling the syringe and tubing with liquid are included . The system comes complete with a 100 µL gas tight syringe and other syringe sizes can be purchased .

solid stainless steel frame

Hand-held and battery operated, PM Series pressure manometers monitor vacuum and pressure in nonaqueous

fluids . An integral transducer and digital display allow easy and accurate pressure readings . Three versions

measure pressures in the range of ±1 psi, ±15

psi or ±100 psi . A range

switch allows measurement in units of psi

or kPa for the 100 psi version, and psi or mm

Hg for the 15 psi version . Pressure can be read on the built-in LCD display or relayed to a chart recorder, oscilloscope, or computer .

PM Series pressure manometers come with 4 feet of 1⁄8-inch ID soft vinyl tubing . A mini-phone-to-BNC cable for the recorder output is also available (Part #CBL102) . Standard versions are equipped with a nine-volt alkaline battery .

PRESSURE MANOMETER SPECIFICATIONS PM01 PM015 PM100PRESSURE RANgES ±1 psi (±52 mm Hg) ±15 psi (±775 mm Hg) ±100 psi (±690 kPa)MAX . PRESSURE 20 psi (1035 mm Hg) 30 psi (1550 mm Hg) 150 psi (1035 kPa)RESOLUTION 0 .001 psi (0 .1 mm Hg) 0 .01 psi (1 mm Hg) 0 .1 psi (1 kPa)OUTPUT 1 V/psi 100 mV/psi 10 mV/psi OUTPUT RANgE ±1 .0 V ±1 .5 V ±1 .0 VLINEARITY 0 .5% full-scaleTEMPERATURE EFFECT 1 .0% full-scale (0-70°C)ZERO Screwdriver-adjustRESPONSE TIME 30 msPOWER Nine-volt batteryBATTERY LIFE Alkaline, 200 hours; rechargeable, 25 hoursRECORDER OUTPUT Mini-phone jack, 0 .141 inch (3 .5 mm)OUTPUT IMPEDANCE 1 kΩPNEUMATIC CONNECTORS Barbed, for 1/8-inch or 3/16-inch ID soft tubingDIMENSIONS 3 x 6 x 1 inches (8 x 15 x 4 cm)SHIPPINg WEIgHT 3 lb (1 .4 kg)

Pressure Manometer For measuring hydrostatic pressures



MMP Manual Microsyringe PumpDMP Manual Microsyringe Pump with Digital Display

ACCESSORIESSPG-KIT-MICRONR Software and Serial Cable for Transmitting Data MMP-KIT Injection Assembly Parts Kit

Not including valve—see #14057-10, page 173)

PM01D Pressure Manometer (1 psi) PM01R Pressure Manometer (1 psi), Rechargeable* PM015D Pressure Manometer (15 psi) PM015R Pressure Manometer (15 psi), Rechargeable* PM100D Pressure Manometer (100 psi) PM100R Pressure Manometer (100 psi), Rechargeable* CBL102 Mini-Phone-to-BNC Cable


*Rechargeable versions come with nickel/cadmium battery and charger

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NanoFil™ Sub-microliter injection system for small animal research

Application KitsThese kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and intraocular (IO) in addition to brain injection in mice . They need to be used with a NanoFil syringe and UMP2 to achieve accurate, repetitive, and oil free injection in the submicroliter range . Each kit includes two pieces of Silflex tubing (one for a spare), a holder assembly, spare gaskets, and an assortment of four tips - blunt for the RPE kit and beveled tips for the IO kit . Each kit comes with one each of 33, 34, 35 and 36 gauge tips so that first time users can find the best size for their application .

The Silflex tubing is the most critical component of the kit . This 35 cm long, flexible tubing has a very precise outer diameter for airtight fitting with the syringe . It also has a small inner diameter for minimum dead volume, and is very durable when handled correctly . The SilFlex is coupled to the injection tip with a mechanism similar to that of the NanoFil . The dead volume of the entire kit (including the tubing) is less than 3 microliters . All of the components in the kit are constructed of inert, solvent resistant, and autoclaveable materials for easy cleaning after viral injection .

l The world's smallest dead volume injection syringel Comes with various needle sizes from 26 ga. to 36 ga.l Versatile research applications — RPE and IO Kitsl Custom needle shapes available — blunt, sharp, beveledl Compatible with WPI's UMP2 and PV800 series microinjection systems

NanoFil is a specially designed 10 microliter syringe developed in response to customer requests for improved microinjection in mice and other small animals . It makes quantitative nanoliter injection much easier and more accurate than any other method currently in use .

NanoFil's low dead volume eliminates the need for oil backfilling, a messy process which risks contamination of the injected sample .Injection is now simpler, and less messy, and there is no possibility of oil contamination in critical applications such as ophthalmology research (see the Retinal Pigment Epithelial (RPE) and Intra Ocular (IO) injection kits listed below) .

When the inner tip diameter of a conventional syringe is reduced to less than 100 micron, it is very difficult to backfill the solution at a reasonable speed . NanoFil solves this problem by using a tip coupling mechanism that makes it possible to change the syringe tip during

the experiment . Simply load the sample using a larger tip, such as the 26 gauge needle provided with the syringe, and then replace it with a micro tip for sample injection . On a conventional 10 microliter syringe, a solid ring or bushing is permanently bonded to the tubing . Replacing the tip in middle of the experiment is not practical . With the NanoFil, tips can be exchanged by a simple twist of the brass lock, gently pulling out the tip, and replacing with the desired new tip . To secure the tip, NanoFil uses an olive shaped silicon gasket that is similar to, but much sturdier than, some of the microelectrode holders used for electro physiology recording . The silicone gasket makes it possible to hold not only metal tips

but also glass and quartz tubing . Many types of tubing can be easily connected to the syringe as long as the outer diameter (OD) is close to, but not more than, the inner diameter (ID) of the inside barrel . Flexible quartz capillaries used in gas Chromotography (gC) and Capillary Electrophoresis (CE) can also be easily coupled to the syringe .

Specially designed tips as small as 36 gauge (110 micron OD) are offered in both blunt and beveled styles . Our studies have shown that these tips will cause less trauma to the tissue than any other form of micro syringe currently in use . NanoFil has a unique coupling mechanism that allows many different forms of small tubing and tips to be coupled with the syringe barrel .

RPE-KIT



NANOFIL NanoFil Syringe, 10 microliterNanoFil syringe does not contain any injection tips, those must be purchased separately. It

does include a 26 gauge beveled needle for backfilling.

RECOMMENDED ACCESSORIESRPE-KIT Retinal Pigment Epithelium (RPE) injection kit (SilFlex tubing, gasket, holder, and blunt tipmix) IO-KIT Intraocular (IO) injection kit (SilFlex tubing, holder, gasket, and beveled tipmix)



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“D”

“C”

“E”

“A”

“B”

“F”A

DETAIL A

The replaceable needles used with the NanoFil are available with either blunt or beveled tips . The blunt tip is used for injection into soft tissue and when a uniform solution distribution is needed . The beveled style is used for applications that involve the penetration of a tough tissue .

One of the main factors that limit the resolution and accuracy of conventional micro syringes to the upper tens of nanoliters range is diffusion in the large tip ID . When the tip ID is equal or larger than 100 micron, the error caused by tip diffusion is in the nanoliter range level ( (100 micron)^3 = 1 nanoliter ) . With a 36 gauge needle installed on the NanoFil, the error caused by diffusion will be reduced to the sub nanoliter level, making accurate injection of a nanoliter possible .

All of WPI’s beveled tips have a unique 25 degree tri-surface bevel that is optimized for microinjection . A 10 degree single-surface beveled tip penetrates better than one with a 25 degree angle, however

the distance between the upper opening to the tip (see section “F” in Fig 2) is longer . As a result, it requires a deeper penetration of the tip to achieve the same level of liquid delivery . Deeper penetration means more tissue damage . WPI’s unique 25 degree beveled tip solves this problem with two extra beveled surfaces . The tip of a single surface beveled tip is actually a blade instead of a point . It dulls very quickly . In contrast, the tri-surfaced tip has a real point . It not only penetrates much better but is also much more durable . Our tests show that our 33 gauge, 25 degree beveled tip penetrates easier and lasts longer than other manufacturer’s 33 gauge, 10 degree single beveled tips . With a 35 gauge tri-surface beveled tip, the resistance to the penetration becomes even less . Each of our tips undergo a penetration test before leaving the factory to guarantee the best results for our customers .

Selecting the correct tip for your application

Available Tips33 gauge: This tip is similar to Hamilton’s 7762 and 7803 series removable needles in both tip length and outer diameter . However, our beveled tip version is shorter, more durable, and penetrates better due to the special tri-surface grinding technique . In the past, 33 gauge tips were the smallest size sold by other manufacturers and were frequently cited in literature . However, our new 35 gauge tip is much better for injections involving small animals, especially mice . Compared with Hamilton’ 33 gauge, 10 degree beveled tip, our 35 gauge 25 degree beveled tip can reduce the depth of penetration by almost 80% . The distance between the tip and the upper rim of the opening (section F on Figure 2) is 1024 microns for the 33 gauge tip . The distance for our 35 gauge tip is only 230 microns . In addition, the smaller tip size significantly reduces the required penetration force . In nearly all applications, a 33 gauge tip can be replaced with our 35 gauge tip and produce better results .

34 gauge: This is a transitional size between the 33 gauge and 35 gauge . If the 35 gauge is too weak and the 33 gauge is too large, this makes a good alternative .

35 gauge: This was the most popular and preferred tip of most scientists during our field trial . The combination of its strength, length, durability, and clogging resistance creates a balance with very little compromising of the individual properties . It is much smaller than the 33 gauge tip offered by other manufacturers . It is only slightly larger than the 36 gauge tip but is much stronger and less likely to be clogged . Samples can be directly loaded with this tip . Its 5 mm length is sufficient enough for almost all injection applications in mice .

36 gauge: This is the smallest tip that is commercially available . The tip is so small that it can be inserted into the opening of the 33 gauge needle tip . Because this is pushing the limits of what current technology can produce, there are some limitations to consider before using . Its thin diameter makes it necessary to limit its length to 2 .5 to 3 .0 mm and still maintain a usable strength . Since the tip ID is in the 25 to 50 micron range, it is very easily clogged . Therefore, only well filtered solutions can be used . Depending on the viscosity of the sample, the user might also need to pre-load the syringe with a regular tip before switching to this tip for injection . We recommend using the 35 gauge tip instead of the 36 gauge unless it is absolutely necessary .

Flexifil: The Flexifil tip is made of a titanium alloy . The advantage of this tubing is its durability . This “semi-flexible” tip can be bent up to 90 degrees without damage . It is also much more corrosion resistant than the stainless steel tip . Saline solutions left in the tip will be less likely to clog it . Although this tip is specified as a 33 gauge tip, its outer diameter is slightly smaller than our 33 gauge stainless steel tip .

Flexible Quartz Tubing : The flexible quartz tubing tip is made of 160 micron OD polyimide coated quartz tubing with a special adapter sleeve mounted at the end . It is designed for filling glass capillary electrodes or pipettes, just like WPI’s traditional MF34g Microfil . However, unlike the traditional MicroFil, which has about 50 microliters of dead volume in its luer hub, the dead volume of this tip is less than 0 .6 microliters . It is useful for loading electrodes with solutions that have a limited volume or are too expensive to waste .

Tip Order Number

Tip O.D. “A”

Tip I.D. “B”

Tip Length

“C”

Total Length

“D”

Shank O.D. “E”

Tip Material

NF33BV-2 210 µM 115 µM 10 .0 mm 40 .0 mm 460 µM Stainless Steel




NF33FBV-2 200 µM 100 µM 5 .0 mm 35 .0 mm 460 µM Titanium Alloy

NFQ34-5 160 µM 100 µM 55 .0 mm 75 .0 mm 460 µM Quartz

Above dimensions apply to blunt tips also.



OPTIONAL ACCESSORIESNF33BL-2 33 g blunt NanoFil needle (pkg of 2) NF34BL-2 34 g blunt NanoFil needle (pkg of 2) NF35BL-2 35 g blunt NanoFil needle (pkg of 2) NF36BL2 36 g blunt NanoFil needle (pkg of 2) NF33BV-2 33 g beveled NanoFil needle (pkg of 2) NF34BV-2 34 g beveled NanoFil needle (pkg of 2) NF35BV-2 35 g beveled NanoFil needle (pkg of 2) NF36BV-2 36 g beveled NanoFil needle (pkg of 2) NF33FBL-2 33 g Flexifil blunt NanoFil needle (pkg of 2) NF33FBV-2 33 g Flexifil beveled NanoFil needle (pkg of 2) NF33-36BL Assortment of 4 blunt NanoFil needles NF33-36BV Assortment of 4 beveled NanoFil needles NFQ34-5 34 gauge Flexible Quartz Tubing for filling (pkg 5)

Replacement PartsNFINHLD NanoFil Injection Holder SILFLEX-2 SilFlex tubing (pkg of 2) NFGSK-5 Spare Silicone gasket for NanoFil & Holder (pkg of 5)

Fiber Optic Spectrophotometers and Spectrometer Systems TIDAS I—spectrometerforlow-noiseUV/VISabsorbanceapplications............... 244

UltraPath™—exceptionallyhighdynamicrangeUV/VISspectrometersystem. . . . . . . . 248

SpectraUSB4—miniaturefiberopticspectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . .250

Sample Cells

MicroliterSampling LWCCLiquidWaveguideCapillaryCell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252

SPTSpectroPipetter™forUV/VIS/NIRapplications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254

DipTip™—UV/VIS/NIR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255

MilliliterSampling StandardCuvetteHolders[1cm&10cm]. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256

4-WayCuvetteHolder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256

Cuvettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257

TransmissionProbes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .258

Fiber Optic Light Sources FO-6000HighPower,LowDrift(VIS)TungstenLightSource. . . . . . . . . . . . . . . . . . . . . .260

D2H™DeuteriumHalogenLightSource(UV/VIS/NIR). . . . . . . . . . . . . . . . . . . . . . . . . . . .260

D2Lite™Miniature(UV/VIS)DeuteriumLightSource. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 261

LED-Lite™plusELSmodulesforsinglewavelengthapplications . . . . . . . . . . . . . . . . . . 261

Accessories FiberOpticCablesandAssemblies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259

In-LineFilterHolderAssembly—SMAorSTconnections. . . . . . . . . . . . . . . . . . . . . . . . . 262

FiberOpticCollimator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 262

LinearVariableFilters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .256

Fluorometry SpectraUSB4-FLGPreconfiguredModuleforFluorescenceApplications . . . . . . . . . . .250

Fiber Optic Sensors FOMSBloodPressureMeasurementSystemforSmallandLargeAnimals . . . . . . . . . . . 43

OxyMicroandOxyMiniOxygenSensorswithLuminescenceLifetimeDetection. . . . . 95

pHOpticapHSensorSystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

Reagent Kits PEROXYLUMINOLHydrogenPeroxideDeterminationKit. . . . . . . . . . . . . . . . . . . 90,262

GSHAssayKitforQuantitativeDeterminationofGlutathione. . . . . . . . . . . . . . . . . .91,263

GriessReactionNitriteKit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92,264

NITRALYZER-II,NitratetoNitriteReductionKit. . . . . . . . . . . . . . . . . . . . . . . . . . . . 92,264

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Spectroscopy





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WPI offers complete pre-configured systems for a variety of applications from protein analysis to flow-through colorimetric detection in the UV-VIS-NIR spectral region. It is to the customer’s advantage to consider purchasing a complete system. Ordering a pre-configured system eliminates the guesswork of ordering parts and offers the best pricing. In addition, each system has many hours of testing in our research labs by WPI’s scientists.

If you are not sure that a complete system is what you need, we recommend calling and speaking with one of our technical product specialists.

We can then configure a customized system especially for you with our complete line of spectroscopy products and accessories. Select a detector based on your own individual application. WPI offers a complete range of CCD (charge coupled device) and PDA (photodiode array) modules.

Choosing the right detector will depend upon the

accessories you need, budget, and computer interface. WPI offers an array of innovative fiber optic sampling cells. Many are designed and protected by patents issued to WPI. As the table above shows, customers may choose a sensor cell based upon sample volume and detection limits.

Choosing a Preconfigured SystemSystem Detector Detection Sample PrincipleApplication(s) Page

Range(nm) Volume

TIDAS1-LWCC PDA 250-730 2.5µL/cm Nutrientanalysis,tracemetaldetection,waterpurity 247

TIDAS-QUAD PDA 230-730 Varies 4-channeldetectionsystem 247

TIDAS1-SPT PDA 230-730 2µL Protein(DNA/RNA) 247

TIDAS1-TIP PDA 230-730 10-30µL Dissolutionstudies,multipurposetesting 247

UPVIS (UltraPath) PDA 380-730 10mL Openocean(CDOM)detection,broaddynamicrange 248

UPUV (UltraPath) PDA 250-730 10mL Openocean(CDOM)detection,broaddynamicrange 248

SPECTRAUSB4-LWCC CCD 350-730 2.5µL/cm Colorimetricdetection.VISsystem 251

SPECTRAUSB4-LWCC-UV CCD 250-730 250µL FlowthruanalysiswithUV-VIScapability 251

SPECTRAUSB4-SPT CCD 230-800 2µL Lowestvolumesampling,protein,UV-VISmultipurpose 251

SPECTRAUSB4-FLG CCD 380-1050 1-3.5mL Fluorescence 251

SPECTRAUSB4-CUV CCD 250-800 0.2-3.5mL Cuvette-basedsampling,multipurpose 251

• Preconfigured systems for specific applications *

• User-configured systems

• Fiber optic accessories — light sources, cuvettes, multi-mode and bifurcated patch assemblies, and more

• Biology — Chlorophyll, CDOM, color changes and UV absorption of organic species. See UltraPath on page 248.

• Biochemistry—Fluorescence, DNA/RNA, Rhodopsin studies.

• Chemistry—Determination of phosphates, nitrates, nitrites, metal complexes in aqueous media. See LWCC, page 252.

• Environmental—Water quality (chlorine/nitrate) with intrinsic deep UV and/or VIS colorimetric methods. See LWCC and UltraPath, pages 248, 252.

• Pharmaceutical—Drug development and discovery, process control. TIDAS-QUAD — call for details.

*

Spectroscopy

PricesshownareinU.S.dollars.Actualchargeswillvarybecauseofimportduty,freight,andcurrencyfluctuations.Toobtainanexactquotation,contactyourWPIoffice.


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More light through fitted optical constructionTIDAS I is a high precision detection system based on fiber optic design. All components are made to match to one another and are “calculated” rather than “tried out”. The detectors are monolithic diode array modules, made by Zeiss. Only in the TIDAS can be found a multi-layer Zeiss light filter with maximum light-gain for second order suppression. Detection tolerance is improved in comparison to conventional diode array spectrometers. Most cuvette-based standard spectrometers lose more than 90% of light through expensive prism decoupling. The TIDAS I is designed for fiber optic sampling cells. The TIDAS I spectrometer module is a high performance and low noise detector for modular sampling systems.

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Tidas I

Tidas I is the first high end spectrometer module designed for fiber optic based sampling techniques. Using suitable light sources and sample cells, spectral detection in the wavelength range of 195 to 730 nm can be performed at noise levels as low as 0.08 mAU. Tidas I outperforms conventional bench-based spectrophotometers and CCD-based spectrometer modules, when it comes to high precision fiber optic sampling. Using fiber-optic based sample cells and light sources, high-end detection systems with

TIDAS I SPECIFICATIONSOPTICAL BASICS Monolithic Spectrometer Module, Single Beam System.

Fiber optic cross section converter for increased light throughput instead of entrance slit.Zeiss multilayer filter.Hamamatsu photodiode array detector, 16-bit.

WAVELENGTH RANGE 195 - 730 nmNUMBER OF DIODES 256RESOLUTION 2.2 nm/pixelSPECTRAL BANDWIDTH (FWHM) 5 nmWAVELENGTH ACCURACY ± 0.2 nmWAVELENGTH REPRODUCIBILITY ± 0.07 nmNOISE AT 550 NM ± 0.8 x 10-5 AU (using FO-6000)BASELINE DRIFT Light source dependentCOMPUTER INTERFACE Serial RS-232 interfaceSYSTEM REQUIREMENTS Windows 2000/XPDIGITAL INPUTS/OUTPUTS 5/5DIMENSIONS 6.3 x 10.6 x 12.8 in.

16 x 27 x 32.5 cmELECTRICAL REQUIREMENTS 90-240 V, 50/60 HzWEIGHT 18 lb (8 kg)

exceptional sensitivity and noise performance can be easily assembled. Using our liquid waveguide capillary cells (LWCC), absorbance based detection system with effective pathlengths as long as 500 cm and noise levels as low as 0.1 mAU can be achieved at reasonable cost. Microliter sampling systems for UV/VIS applications can be assembled, using our SpectroPipetter (SPT-2), or DipTip™dipping probe. See next page for software details; see page 247 for complete detection systems.

● Photodiode array spectrometer● Low noise detection ● Wavelength range 195 nm to 730 nm● Fiber optic design

PricesshownareinU.S.dollars.Actualchargeswillvarybecauseofimportduty,freight,andcurrencyfluctuations.Toobtainanexactquotation,contactyourWPIoffice.


TIDASI Tidas I Spectrometer Module with TidasDAQ/SpectraView





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Highperformancefiberopticspectrometersystem

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SoftwareWPIoffersaninstrumentdriver,TidasDAQ,whichcollectsdatafromTidasIandimportsintoGrams/AIorSpectraView.Botharecomprehensivedataprocessinganddatamanagementsoftwarepackages.TidasQuadisaninstrumentdriver,whichcanmanageuptofourchannelssimultaneouslyusingasingleTIDASIspectrometermodule.TidasDAQandTIDAS-QUADcollectdatawhichcanbeimportedandprocessedinGrams/AIorSpectraView.

TidasDAQ, a unique software package for TIDAS-IWithTidasDAQ,highprecisionabsorbanceortransmittancespectracanbeobtainedinlessthanaquarterofasecond.Onlyafewparametersneedtobeadjustedtoobtainandanalyzespectraldata.TheTidasDAQ

softwarepackageeasilyacquiresspectra.SpectraViewincludestoolsforsamplequalification,quantification,validationandqualitycontrol.Datacanbeimportedfromandexportedtodifferentspectrophotometerformats.Theproject-basedapproachoftakingsampleswithinanexperimentallowseasydocumentation.SpectraViewanditsoptionalanalysispackage(Grams/AI)offerfurtherconcentrationmeasurements,coloranalysis,enzymeactivityandmanyothers.Extensivecalculationfeaturesallowtoprocessyourdata(derivatives,smoothing,log,normalized,baselineandpeakanalysis).Averyusefulfeatureofthesoftwareisitsextensivekineticsfunctions.

Above: SpectraView 3D view. Spectra can be displayed and analyzed in 2D or 3D format (as shown). This allows the user to interpret “time-aquisiton” data typically done with a

TiDAS-1-LWCC flow system.

At left: SpectraView 2D overlapping function allows one to view and analyze a set of data. in this case, a series of calibration samples for a nitrite calibration set measured at 540 nm.

TidasDAQ acquisition window, showing an absorbance spectra of phenol red dye acquired using the TiDAS-1-LWCC system setup with a 10-cm path flow cell.



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Thekeytosuccessfullyanalyzingyoursampleisamatchedsamplecell.ThefiberopticbasedTidasIspectrometermoduleoffersmanypossibilities,fromusingstandardcuvettesamplingforconventionalUV/VISworktousingalongflowpathcell(LWCC)upto500-cminlengthforimprovedsamplingsensitivityforallcolorimetrystudies.Severaltypicalandwell-provensystemsaretheirapplicationsareshownbelowandonthefollowingpage.

Selectionofanappropriatesamplecelliskeytothesuccessfulanalysisofyoursample.WPIpridesitselfoninnovativefiberopticsamplecellsoptimizedforaparticularapplication.Nutrientanalysis,microvolumeproteindetection,tracemetalanalysis,flowcolorimetryanddissolutiontestingareonlysomeoftheareasWPI’scellshavebeensuccessfully

used.Sampleproperties,volume,analyteconcentration,wavelengthrangeandpathlengthdetermine,whichofWPI’suniquesamplecellyoucanuseinyourresearch.

ThetablebelowprovidesanoverviewofWPI’swiderangeofopticalsamplecellsandtheirpotentialapplications.

Selecting a Sample Cell

TIDAS I & SpectraUSB4 Systems

Cell Type Sample Volume Pathlength [cm] Range* [nm] Application Page

LWCC Flowcell 2.5µL/cm 2to500 200-1000 Nutrientanalysis,tracemetaldetection,colorimetrics... 252

UPATH Multiplepathflowcell 10mL 2,10,50,200 230-730 Naturalwater,CDOM,tankcleaning... 248

SPT Microliterdippingprobe 2µL 1 230-1000 Protein,DNA/RNA,rhodopsin... 254

DipTip Miniaturedippingprobe 30-50µL 0.2,0.5,1 230-1000 Protein,DNA/RNA,rhodopsin,dissolution 255

CUV-UVCUV-UV-FLCUV-UV-10

CUV-ALL

Cuvetteholder,2-&4-way 0.2-3.5mL 0.5,1,10 200-1000 Absorbanceandfluorescencewithstandardandflowcuvettes 256

TR600 Highefficiencytransmissionprobe 5mL 0.2,0.5,1,2 200-1000 Colorimetrics,standardlabuse,onlineanalysis 258

TR-UV Dippingprobe 5mL 0.2,0.5,1 230-1000 Colorimetrics,standardlabuse,onlineanalysis 258

REF-UV Reflectanceprobe NA NA Scattering,reflectanceanalysis 258

*Range depends cell version [UV, VIS, NIR], light source and detectors



TIDAS SYSTEMS UV VIS NIR D2H FO-6000Fiber Optic Interface

Start-up Kit

Vol Req’dDynamic

Range (AU)

TIDAS-I-LWCC ● ● 400µSMA 1 250µL 0.005-1.2

TIDAS-I-LWCC5 ● ● 400µ 1 1250µL “

TIDAS-2-LWCC ● ● 400µ 1 250µL 0.002-2.0

TIDAS-2-LWCC5 ● 400µ 1 1250µL “

TIDAS-I-SPT ● ● ● incl 2 2µL 0.005-1.2

TIDAS-2-SPT ● ● incl 2 2µL 0.002-2.0

TIDAS-I-TIP ● ● ● incl 2 20µL 0.005-1.2

TIDAS-1-TIPUV ● ● ● incl 2 20µL 0.005-1.2

TIDAS-QUAD see website ● ● ● 300µbundle KitOpt 5-500µL 0.005-1.2

Start-up Kit #1KITLWCC includes:500291(Peri-StarPump),501609(cleaningkit),58006(sampleinjectorassembly),FO-400-SMA1M (2)(fiberopticcables)

Start-up Kit #2 UCKincludes:PUC-Y(ultrasonicbath),specialstand,and15807(ultrasoniccleaningsolutionconcentrate)

SPECTRAUSB SYSTEMS UV VIS NIR D2LITE FO-6000Fiber Optic Interface

Start-up Kit

Vol Req’dDynamic

Range (AU)

SPECTRAUSB-LWCC ● ● 400µcore 1 250µL 0.010-1.5

SPECTRAUSB-SPT ● ● ● NA 2 2µL “

SPECTRAUSBFL ● ● 600µcore NA 3mL “

SPECTRAUSB-CUV ● ● ● 400µcore NA 3mL “



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TIDAS-QUADisafour-channelUV/VISdetectionsystemforflowanalysiswithWPI’sliquidwaveguidecapillarycells(LWCC).Itcanbeconvenientlyusedfornutrientanalysis,microvolumeproteindetection,tracemetalanalysis,

flowcolorimetry,dissolutiontestingortraceanalysisofpharmaceuticalcompounds.Someadvantages,comparedtoothercommerciallyavailablesystems,aresensitivity,ultralownoise,fullautomationandgreatversatility.ThebasiccomponentsofthesystemareaTIDASIphotodiodearrayspectrometer(range:230-730nm;resolution:5nmFWHM;noise:5µAU/cm),fourlightsources(D2Lite),fourLWCCs(100cm),ancillaryfibersandcablesandaninstrumentdriver,interfacingthespectrometertoadataanalysispackage(Grams/AI).TheTidasIconnectstoacomputerviaRS232interface.Thissystemisdesignedforfiberopticuse;mostofWPI’ssamplescellscanbeused.

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TIDAS-I-LWCCHighsensitivitydetectionsystemsforabsorptionmeasurementswithopticalpathlengthsrangingfrom2to500cmcanbeconfiguredusingaTIDASI.

TIDAS-I-SPT TIDAS-I-TIP

Thesedetectionsystemscanbeusedinavarietyofapplications,suchasstoppedflowdetection,fluidinjectionanalysis,drinkingwateranalysis,aswellasenvironmentalandoceanographicanalysis.TheTIDASIoffersfivedigitalinputsandoutputs.

TIDAS-I-LWCCsystemcontains:TIDAS Ispectrometermodule,aLiquidWaveguideCapillaryCell(LWCC-2100),aFO-6000visiblelightsource,twoopticalfibers(FO-400-SMA1M)andaPeriStarProperistalticpump(500291).SimilarUVsystemsareavailableonrequest.

WPI’ssmallestandeasiesttousemicroliterspectroscopicsamplecellistheSpectroPipetter™.Only2µLofsamplearedrawnintothecelltomeasureasamplespectra.MicrolitersamplingofDNAandproteinsamplesorcolorimetricsintheUVorVIScanbeconvenientlyperformedwithWPI’sSpectroPipetter.

TIDAS-I-SPTsystemcontains:TIDAS Ispectrometermodule,D2HUV/VISlightsource,SpectroPipetter™(SPT-2),ultrasoniccleaningkit(UCK).

WPI’sminiatureUV/VISdippingprobe,DipTip™,withatipdiameterof1.5mm,fitsdirectlyintoallmicrocentrifugevialsonthemarket.MicrolitersamplingofDNAandproteinsamplesorcolorimetricsintheUVorVIScanbeconvenientlyperformedwithaDipTip.AtypicalsamplesystemforVISapplicationsconsistsofTIDAS Ispectrometermodule,FO-6000,DipTip™andanultrasoniccleaningkit(UCK).ForUV,aD2Hdeuterium/tungstenlightsourceisusedinsteadoftheFO-6000.

TIDAS I & SpectraUSB4 Systems

TIDAS-QUAD High performance UV/VIS 4-channel flow detection

● Process Control Drug Discovery Trace Analysis

● Low Noise: 5 µAU/cm @ 550nm

● Easy to Use, Fully Automated

● Meets Pharmaceutical Requirements (21 CFR 11)



TIDAS-I-SPT TIDASI-SPTSystem

TIDAS-QUAD TIDAS-QUADSystem

Seewebsiteforfulldetails.

TIDAS-I-TIP TIDASI-DipTipSystemTIDAS-I-TIPUV TIDASI-DipTipSystem

TIDAS-I-LWCC TIDASI-LWCCSystem(100cmpathlength) TIDAS-I-LWCC5 TIDASI-LWCCSystem(500cmpathlength)

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UltraPathisauniquehigh-performancespectrophotometerofferinguser-selectableopticalpathlengthsof2,10,50and200cm.Designedforthedetectionoflowabsorbingspeciesinaqueoussolutions,UltraPathisanidealtoolforanystudyrequiringpreciseandhighlysensitivespectroscopicdeterminationofanalytes,eitherinthelaborinthefield.Theinstrumentoperatesinthewavelengthrangeof380to730nm(UPVIS)or250to730-nm(UPUV)andhasanexceptionaldynamicrangeallowingreliableabsorbancemeasurementsofbetween5µAU/cmto1AU/cmtoberoutinelymade.

BackgroundUltraPathwasdevelopedbyWPIunderacollaborativeagreementwithNASA(StennisSpaceCenter)forthespectroscopicdeterminationofcoloreddissolvedorganicmatter(CDOM)inseawaterandfreshwaterenvironments,andwasdesignedforuseinthelaboratoryandinthefield(i.e.,atsea).CDOMconcentrationsvarysignificantlybetweenopenoceansampleswithlowCDOM(e.g.,0.007m-1at380nm),andhighCDOMfreshwaterenvironments(e.g.,10-20m-1at380 nm).ToaddresstheseproblemsthedesignrequirementsofUltraPathmandatedthedevelopmentofaruggedportablesystemcapableofhighsensitivitymeasurementsacrossawidedynamicrange.

TheUltraPathsystemmeetsthesestringentdesigncriteriaandenablesreliablemeasurementofCDOMintherangeof0.002m-1to200m-1(380to730nm).Fig1.illustratestwotypicalabsorptionspectrameasuredusingUltraPath.Thesamplelabeled“High”isahighlyconcentratedCDOMsamplewithlowsalinitycollectedfromamarshdrainageareaofanurbanlake(LakePontchartrain,Louisiana).TheCDOMsamplelabeled“Low”isfromoligotrophic,lowproductivewaterswithhighsalinitycollectedofftheWestcoastofPuertoRicointheMayagüezBay.SpecialattentionshouldbedrawntotheinsertofFig.1,

• Rugged system for onboard measuring • Reduces cost and expense • Highly sensitive and stable • Portable •

Easy to use • Provides user selected optical path lengths • Proven to work in divergent environments

UltraPath™

Auniquemultiplepathlengthsamplecellforabsorbancespectroscopywithextendeddynamicrange

Fig. 1— “HIgH” CDOM is from a low salinity marsh environment (Lake Pontchartrain, Louisiana). “LOw” CDOM was collected from the high salinity oligotrophic waters of Mayagüez Bay on the west coast of Puerto Rico. Data courtesy of NASA Stennis Space Center.

400 500 600 7000

1

2

3

4

LOW

HIGH

Abs

orp

tion

[m-1

]

Wavelength [nm]

400 500 600 7000.00

0.02

0.04

0.06

0.08

LOW

HIGH

Abs

orpt

ion

[m-1]

Wavelength [nm]

whichrevealstheexceptionalsensitivityofUltraPathenablingdetectionofCDOMabsorptionbelow0.03m-1.Basedoncustomerrequest,WPIhasdesignedanUltraPathsystemforUVandVISapplications,theUPUV.





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400 500 600 700

0.000

0.005

0.010

0.015

0

25

50

75

2 nM

Ab

sorb

ance

[µA

U/c

m]

Spectral Detection of Ponceau SM

easu

red

Ab

sorb

ance

[AU

]

Wavelength [nm]

ApplicationsThedesignofUltraPathissuchthatitwillhaveanimportantuseinanyapplicationsrequiringpreciseandhighlysensitivespectroscopicdeterminationofanalytesacrossawidedynamicabsorptionrange(i.e.,5µAU/cmto1-AU/cm),eitherinthelaborinthefield.TofurtherillustratetheuniqueperformanceofUltraPath,samplescontainingultra-lowconcentrationsofPhenolRedweremeasured.Fig.2illustratesclearlythedetectableabsorptionspectraobtainedbetween0.9nMand4.6nMPhenolRed.ThekeydesignfeatureofUltraPathisthattheopticalpathlengthcanbeeasilychangedtocoveradynamicrangenotpossibleusingastandardsinglepathlengthsamplingsystem.

DesignUltraPathhasfouropticalpathlengthscontainedwithinasinglesamplecell(i.e.,2cm,10cm,50cmand200cm).Thepathlengthsareuser-selectable,offeringaveryhighsensitivityandanextendeddynamicrangeforVISabsorbancemeasurements.Auser-controlledswitchchangestheopticalpathlengthofthesamplecellallowingabsorbancemeasurementsoverabroaddynamicrange.Thefluidpathofthesamplecellisoptimizedtoproducealaminarflowthatisvirtuallyfreeofinterferencefromtrappedairbubblesandadherenceofdissolvedsubstancestothecellwall.Inparticular,thedesigngreatlyminimizestheproblemscommonlyfoundwithflowcellsoflongopticalpathlengths:theriskoftrappingdustparticles,fibersorparticulatematterinsidethecell.

TheUltraPathsystemincludesaphotodiodearray-basedspectrometermodule(TIDASI)tomeasuretheabsorbanceattheselectedpathlength.Absorptionoflightismeasuredbetween370nmand730nmwavelength(UPVIS)and250nmto730nm(UPUIV)witharesolutionof5nm(FWHM)andanoiselevelbelow0.2mAU.Afiberopticlightsourcedeliverslightviaopticalfiberstothemultiplepathlengthsamplecell.Aperistalticpump

Fig. 2 – Ponceau S absorption measured with UltraPath (200 cm cell). Ponceau S was dissolved in Millipore water. A single absorbance measurement was made. The signal was neither smoothed nor averaged. Absorbencies are shown on the left ordinate in measured absorbencies [AU] and on the right ordinate in normalized absorbencies [AU/cm].

ULTRAPATH SPECIFICATIONS UPVIS UPUV

DYNAMICRANGE 5µAU/cmto1AU/cm) 5µAU/cmto1AU/cm) 0.002m-1to200m-1 0.002m-1to200m-1

WAVELENGTHRANGE 380–730nm 250–730nm

WAVELENGTHRESOLUTION 5nm(FWHM) 5nm(FWHM)

NOISE(PEAKTOPEAK) <0.2mAU <0.2mAU

DRIFT <0.5mAU/h <0.5mAU/h

OPTICALPATHLENGTHS Selectable:2cm,10cm,50cm Selectable:2cm,10cm,50cm and200cm and200cm

INNERDIAMETER ≈2mm ≈2mm

CELLVOLUME(FILLING) ≈10mL(at200cmpathlength) ≈10mL(at200cmpathlength)

SAMPLEINLET/OUTLET 1/8”tubing 1/8”tubing

FIBERINPUT 600µmcorediameter,SMA 600µmcorediameter,SMA

FIBEROUTPUT 400µmcorediameter,SMA 400µmcorediameter,SMA

SOLVENTRESISTANCE Mostorganic&inorganicsolvents Mostorganic&inorganicsolvents

SYSTEMSHIPPINGWEIGHT 33lb(15kg) 44lb(20kg)

(PeriStar)isutilizedtoevenlydrawsampleupintothesampleinjectorandthroughtheUltraPathsamplecell.

AstandardPCorlaptop(notincluded)isconnectedtothedetectionsystemviaRS232interface.AnRS232-to-USB2.0adapterisnowincludedforconnectionofTidasIspectrometertoanyUSBport.TheTidasDAQinstrumentdriverdisplaysdatawhichmaythenbeimportedintotheSpectraViewdataanalysissoftware(included).

MobilityThesystemisdesignedformobility.ThecomponentsoftheUltraPathsystemaredesignedtofunctionoverabroadrangeoflaboratoryandfieldenvironments.Theelectricalcomponentsaredesignedforlowpowerconsumption.AlaptoprunningWindows98,Windows2000orWindowsNT4.0canbeusedtorunthespectrometeraswellasanalyzedataoutput.

ReferenceRichardL.Miller,MathiasBelz,CarlosDelCastillo,RickTrzaska,“DeterminingCDOMAbsorptionSpectrainDiverseCoastalEnvironmentsUsingaMultiplePathlength,LiquidCoreWaveguideSystem”,Continental Shelf Research,July2002,22:9,p1301-1310.

RichardL.Miller,MathiasBelzandSuYiLiu,“MeasuringtheabsorptionofCDOMinthefieldusingamultiplepathlengthliquidwaveguidesystem”,OceanOpticsXV,paper1308,Monaco,October2000.

“SystemAnalyzesWaterSamplesatSea”,NASA Aerospace Technology Innovation,2001,9(5).http://nctn.hq.nasa.gov/innovation/innovation95/3-techtrans2.html

R.L.Miller,C.Hall,C.DelCastillo,B.McKeeandM.Dagg.“Bio-opticalPropertiesoftheMississippiRiverPlumeandAdjacentShelf.”ASLOAquaticSciences,Albuquerque,NM,2001.

R.L.Miller,C.E.DelCastillo,R.T.Powell,E.D’SaandB.Spiering.“MappingCDOMconcentrationinwatersinfluencedbytheMississippiRiverplume”,ProceedingsofOceans2002,Biloxi,MS.

R.L.MillerandE.D’Sa.“EvaluatingtheinfluenceofCDOMontheremotesensingsignalintheMississippiRiverBight”.InEosTransactionsAGUOceanSciences,2002.Honolulu,HI,p.171.

D’Sa,E.,R.L.MillerandR.Trzaska.“AparentOpticalPropertiesinWatersInfluencedbytheMississippiRiver”,ProceedingsoftheSeventhThematicConference,RemoteSensingforMarineandCoastalEnvironments,2002,6pg,Miami,FL.



UPVIS UltrapathSystem,VisibleLightUPUV UltrapathSystem,Ultraviolet&VisibleLight

The UltraPath system includes: Multiple pathlength cell, Tidas I spectrometer module with TidasDAQ/SpectraView software, FO-6000 light source, 400 µm and 600 µm optical fibers,

8-roller peristaltic pump, silicone tubing, sample injector and waveguide Cleaning Kit.

Specifylinevoltage

501609 WaveguideCleaningKit

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thestandardmirrorsoftheopticalbenchwerereplacebyAg-coatedmirrors.Thisimprovedthesensitivityofthedetectorbyover20%.

TheSPECTRAUSB4-M2isabasicspectrometer,equippedwithagratingoryourchoice.Detectorupgrades(Slit,L4,UV4and/orOFLV4)canbeselectedwhenorderingtheinstrument.Ifanapplicationrequiresaspecificwavelengthrange,resolutionorsensitivity.ContactWPIforcustomization.

High Resolution SpectrometersWPIalsooffersahigh-resolutionversion,theSPECTRA4-HR,oftheSPECTRAUSB4miniaturespectrometerseries.Itskeyadvantageistheexcellentopticalresolutionof0.035nm(FWHM).TheSPECTRA4-HRisespeciallysuitedforapplicationssuchaswavelengthcharacterizationoflasersandLEDs,monitoringofgasesandmonochromaticlightsources,anddeterminationofelementalatomicemissionlines.TheSPECTRA4-HRcanbeequippedwithallupgradesavailable

fortheSPECTRAUSB4-M2.ContactWPIforcustomization.Forwidewavelengthrangeapplications,WPIalsooffersthehighresolutionspectrometer(SPECTRA4-HR-CG)withaninstalledproprietarycompositegrating,a5µmslit,UV4upgradeandorder-sortingfiltertoprovidea200-1100nmwavelengthrangewith1.0nmopticalresolutioninonespectrometer.

a200-850nmwavelengthrangeandcomeswithaUV4-enhancedUVdetectorupgrade,amulti-bandpassorder-sortingdetectorcoating,alongwitha25µmentranceslitinstalledforanopticalresolutionto1.5nm(FWHM).TheSPECTRAUSB4-NIRispresettoa350-900nmrangeandisequippedwitha25-µmslit.Inaddition,weoffersnap-on,directattachaccessoriesspecificallydesignedforthesespectrometers.USBVISandUSBUVarelightsource/cuvetteholdercombosforabsorbancemeasurementsinthe390-950nmforVIS/NIRrangeand200-950nmUV/VIS/NIRrangerespectively.

SPECTRAUSB4-FLGispre-setforfluorescenceapplications.ItisequippedwithanL4lensanda200µmslit.Integratedintothisdetectorisatime-gatedmodethatdelaysthestartofspectraldataacquisitionby5-500microsecondsaftertheexcitationsourceisturnedon.Usingapulsedlightsource,thedetectorseesinthismodethesamplewhenthelightisswitchedoff.However,inordertousethegatedphosphorescencemode,thefluorophoremusthavealongfluorescencelifetime.Thoseworkingwithlanthanidesandphosphorescentmaterialswillfindthegatedphosphorescencemodeparticularlyuseful.Tofurtherimprovetheefficiencyofthedetector,

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Miniature SpectrometersWPI’sSPECTRAUSB4miniaturespectrometersareoptimizedforabsorbance,fluorescenceandNIRapplications.TheyplugdirectlyintotheUSBportofanydesktopornotebookPC.WiththeSPECTRAUSB4,gettingstartedisaseasyasinstallingtheSpectraSuitesoftwarepackage

andconnectingthespectrometertotheUSBportofthecomputer.

WPIoffersdifferentgratingsforthespectrometer,thesedefineitswavelength/detectionrange.Theresolutionofthespectrometerisdeterminedbyitsslit-width(SLIT-xxx).Thesespectrometerscanalsobeequippedwithanumberofdetectorupgrades:acollectionlens,L4,canbeplacedinfrontofthedetectorarraytoincreasethespectrometerslightcollectionefficiencyandreducestraylight.Thisisespeciallyusefulwhenusinglargecorediameterfibersinlowlight-levelapplications.ACCDarraywith3096pixelsisusedasthisdetector.TheUV4isadetectorupgrad,whichisrequiredformeasuringatwavelengthsbelow350nm.Multi-bandpassordersortingfiltersareavailabletoavoidsecondordereffectsinthespectrometermodules(OFLV4).

TheSPECTRAUSB4spectrometerispre-setto

Fiber Optic Spectrometers

SPECTRAUSB4-SPT

SPECTRA4-HR

SPECTRAUSB4+USBVIS



SINGLE-CHANNEL DETECTORSSPECTRAUSB4 200-850nm,25µmslit,1.5nmresolutionSPECTRAUSB4-FLG 380-1050nm,200µmslit,~10nmresolutionSPECTRAUSB4-NIR 350-1000nm,25µmslit,~1.5nmresolutionSPECTRAUSB4-M2 PlugandPlayUSBModule(Call for modifications)SPECTRA4-HR HighResolutionSpectrometerSPECTRA4-HR-CG HighResolutionCompositeGratingSpectrometer200-1100nm,5µmslit,1.0nm(FWHM)resolutionvalue

DETECTOR UPGRADESL4 LowLightUpgrade:DetectorCollectionLensforUSB4000(perchannel)UV4 UVDetectorUpgradeforUSB4000Series,requiredformeasuringatwavelengthslessthan350nm(perchannel)OFLV4-200-850 Multi-bandpassorder-sortingdetectorcoating(perchannel)— UV4 is includedOFLV4-350-1000 Multi-bandpassorder-sortingdetectorcoating(perchannel)SLIT-xxx 5,10,25,50,100,200µm(perchannel)

SeeWPIwebsiteforgrating,slitandresolutionoptions.

ACCESSORIESUSBVIS AttachableLightSource[VIS:390-900nm]+CuvetteHolderUSBUV AttachableLightSource[UV:200-1100nm]+CuvetteHolderUSBCON AttachableLightSourceAdapterforSPECTRAUSB



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DIMENSIONS 89x64x34mm(3.5x2.5x1.31in.)

WEIGHT 200gm(0.45lb)

DETECTOR ToshibaLinearCCDArray

DETECTIONRANGE 200-1100nm(userspecified)

PIXELS 3648

SIGNAL-TO-NOISE 300:1

A/DRESOLUTION 16-bit

SENSITIVITY 130ph/countat400nm; 60ph/countat600nm

ENTRANCESLITS 5to200um;noslitalsoavailable

GRATINGS UVtoShortwaveNIRofferings

FIBEROPTIC SMA905for0.2NAfiberoptics

RESOLUTION 0.3to10.0nm(FWHM)dependentonslitwidth

STRAYLIGHT Lessthan0.05%at600nm;0.10%@435nm

POWERCONSUMPTION 250mA@5VDC

OPERATINGSYSTEM Windows98thruXP,MacOSX,Linuxwith USB,orany32-bitWindowsOS

OPERATINGSOFTWARE SpectraSuite

INTERFACE USB2.0(1.1compatible);RS-232offered

DATATRANSFERRATE Fullscansintomemoryevery13ms. Acquisitionapproximatelyevery25ms.

New Spectroscopy Software WPInowoffersanewacquisitionsoftwaretointerfacewithallSPECTRAUSBspectrometerstoanyPC.SpectraSuitewilloperateinMac,Linux,orWindowsenvironments.WritteninJavaScript,thenewsoftwareisspectrometerindependent—thesamecodewillworkforallUSB-basedspectrometers.CurrentusersofSpectraWaremayupgradetoSpectraSuitebutmustprovidetheserialnumberoftheUSBspectrometertheypurchased.

Software Features●ChemicalReactionKinetics●Zoominginoroutforviewingparticulardatasegments.●AutomaticWindowresizing●Spreadsheetcompatiblesavingformat

(ASCII,GRAMS/SPC,JCAMP)●21CFRPart11Compliant●Internationalconversionmodulesareavailable.

Hardware RequirementsPCwithUSBconnectioninthefollowingoperatingenvironments:Windows98,2000,ME,XP;LinuxRedHat9orlater,Fedora(anyversion),Debian3.1(Sarge),SUSE9.0orlater;AppleMacIntoshOSXversion10.0orlater.30MBofdiskstoragespace;USBport(s)required(mayrunmultiplespectrometersatonce).

SPECTRAUSB4 SPECIFICATIONS

SpectraSuite strip chart display of phenol red absorbance at 559 nm wavelength as a function of time, recorded with LWCC in a flow system.

SpectraSuite phenol red absorbance display as a function of wave-length, recorded with an LWCC flow system and FO-6000 light source.



SPECTRASUITE SpectroscopySoftware SPECTRASUITE-I Spectroscopy Software, Upgrade from SpectraWare™

Spectrometer serial number required.

SPECTRASUITE-U Spectroscopy Software, online updates (1 year)

PRECONFIGURED SYSTEMS

NUTRIENT ANALYSIS (COLORIMETRIC ASSAYS)

SPECTRAUSB4-LWCC Visible/UVDetectionSystem—Includes *SPECTRAUSB4-M2 detector module, MOD-3 – 350-730nm range, 25-micron slit (1.5nm resolution), OFLV4-350-1000, FO-6000, LwCC-2100, plus KITLwCC (Kit includes fibers, pump, cleaning kit) See page 269.

PROTEIN — DNA/RNA SPECTRAUSB4-SPT MicroliterSamplingSystem1--Idealforsmallvolumerequirements—Includes *SPECTRAUSB4 detector module w/ L4 installed, SPT-2, UCK, D2LITE

FLUORESCENCE SPECTRAUSB4-FL FluorescenceDetection(increasedsensitivity)—Includes *SPECTRAUSB4-FLg detector module, FO-6000, CUV-UV-FL, CUV-MSP (2), FO-600-SMA1M (2), CUV2051-1 (or CUV2052-1), 502111, 502112 (See page 273 for more details)

GENERAL PURPOSE CUVETTE SAMPLING

SPECTRAUSB4-CUV UV/VISCuvetteSampling—Includes *SPECTRAUSB4 detector module w/ L4 installed, D2LITE, CUV-UV, FO-400-SMA1M (2), CUV2012-1 (2)

*All systems include wPI’s SpectraSuite software and USB cable

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252252

LWCC SPECIFICATIONS Type II

WAVEGUIDEMATERIAL Fusedsilicatubingcoatedwith alowrefractiveindexpolymer

OPTICALPATHLENGTH 2-500cm

INNERDIAMETER 550µm

INTERNALVOLUME ≈5-1250µL

SAMPLEINLET/OUTLET COMPRESSIONFITTING 1/16”,1/32”

FIBERINPUT ID=400µm

MAXIMUMTEMPERATURE 160ºC

MINIMUMPRESSURE* 1.5-3PSI

SOLVENTRESISTANCE Mostorganic&inorganicsolvents

SHIPPINGWEIGHT 1.4kg(3lb)

*A one-meter Type II waveguide of 550 µm ID requires about 1.5 PSI for water flow of 1 mL/min.

Liquid Waveguide Capillary Cell

UltrasensitiveMicroliterUV/VisSpectroscopy

LiquidWaveguideCapillaryCells(LWCC)areopticalsamplecellsthatcombineanincreasedopticalpathlength(2–500cm)withsmallsamplevolumes(12.5–500mL).Theycanbeconnectedviaopticalfiberstoaspectrophotometerwithfiberopticcapabilities.Ultra-sensitiveabsorbancemeasurementscanbeperformedintheultraviolet(UV),visible(VIS)andnear-infrared(NIR)todetectlowsampleconcentrationsinalaboratoryorprocesscontrolenvironment.AccordingtoBeer'sLawtheabsorbancesignalisproportionaltochemicalconcentrationandlightpathlength.Comparedwithastandard1-cmcell,a1mAUsignalisenhancedfifty-foldwitha50cmcellto50mAU,usingWPI’spatentedaqueouswaveguide

Shown here is a complete system: TIDAS-I-LWCC

Seepage249

Efficiency Curves for LWCC-2

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200 400 600 800 1000

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2-cm5-cm10-cm50-cm100-cm200-cm500-cm

LWCC Key FeaturesPathlength, internal volume, and wavelength range (measured

with ultrapure water and a Tidas II spectrophotometer

Pathlength [cm] Internal Volume [µL]Wavelength Range [nm]

measured with Tidas II

LWCC-2002 2 5 200-1000

LWCC-2005 5 12.5 200-1000

LWCC-2010 10 25 200-900

LWCC-2050 50 125 230-800

LWCC-2100 100 250 230-730

LWCC-2200 200 500 250-730

LWCC-2500 500 1250 280-730

These spectra show the optimal detection limits for LwCCs of varying pathlength.





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253253

with2to500cmopticalpathlength!

technology*.TheLWCCcanbeconnecteddirectlytoapump,achromatographycolumn,orcanevenbefilledusingasyringe.Basedonfiberoptics,theLWCCisdesignedforusewithWPI'sTidas I,Tidas II,orSpectraUSBspectrophotometersystems.Further,modularsamplesystemscanbeassembledusingWPI’sPDA-based(Tidas IorII)orCCD-based(SpectraUSB)spectrometermodulesandWPI’srangeofUV/VISlightsourcesD2H,D2Lite™,andFO-6000 (see pages 254-255).

ApplicationsLWCCshavebeenusedinavarietyofapplications,suchasliquidchromatography,stopped-flowandcolormetricdetection,drinkingwateranalysis,aswellasenvironmentalandoceanographicmonitoringsystems(seeReferencesonWPIwebsite).

WPI’sLiquidWaveguideCapillaryCellsaremadeoffusedsilicatubingwithanoutercoatingofalowrefractiveindexpolymer.Thisresultsinhighsignalstabilityandeasyremovalofairbubblestrappedinthesensorcellduetothehydrophiliccharacterofthecellwall.

Theseuniqueinstrumentswereinvented,patentedandoptimizedbyWPIduringthelastsevenyearsofresearchinthisfield*.Theyfeatureanumberofadvantagesoverconventionalabsorbancecellsandflowcellarrangements.Designedforusewithfiberoptics,theyrequireonlysmallsamplevolumesandhaveahighopticalthroughput.

* Related PatentsMicroChemicalAnalysisEmployingFlowThroughDetectors,1995,U.S.PatentNo.5,444,807.

AqueousFluidCoreWaveguide,1996,U.S.PatentNo.5,507,447.

LongCapillaryWaveguideRamanCell,1997,U.S.PatentNo.5,604,587.

ChemicalSensingTechniquesEmployingLiquid-CoreOpticalFibers,U.S.PatentNo.6,016,372

Mote Marine Laboratories in Sarasota, Florida has partnered with wPI, using the Company’s waveguide technology. wPI customized the LwCC installed in the instrument package inside these AUV’s (Autonomous Underwater Vehicles). The LwCCs are used to monitor the color of the seawater in the gulf of Mexico. One of the primary interest areas of this research is red tide algae blooms.

Waveguide Cleaning Kit (#501609), above, includes the most commonly needed cleaning solutions for the LwCC waveguides. The LWCC Start-up Kit (#KiTLWCC), at right, includes two fiber optic cables (#FO-400-SMA1M), Sample Injector Assembly (#58006), Peri-Star™ Peristaltic Pump (#500291), and waveguide Cleaning Kit (#501609).



LWCC-2002 LiquidWaveguideCapillaryCell,pathlength=2cmLWCC-2005 LiquidWaveguideCapillaryCell,pathlength=5cmLWCC-2010 LiquidWaveguideCapillaryCell,pathlength=10cmLWCC-2050 LiquidWaveguideCapillaryCell,pathlength=50cmLWCC-2100 LiquidWaveguideCapillaryCell,pathlength=100cmLWCC-2200 LiquidWaveguideCapillaryCell,pathlength=200cmLWCC-2500 LiquidWaveguideCapillaryCell,pathlength=500cm

ACCESSORIESAsampleinjectorassemblycanbeusedtoconvenientlyfillanLWCCwithsamplesolutionusingaperistalticpump.PleasenotethattheLWCCrequirestwoopticalfiberstoconnecttospectrophotometersystem.Choosebetweenanti-solarized400microncoreorUV-enhancedcables(maybeorderedin1or3meterlengths).

58006 SampleInjectorAssembly500291 Peri-Star™PeristalticPump,6rollerforsmoothliquidinjectionFO-400-SMA1M FiberOpticcable,1m,SMA,400mmcore,UV-enhanced501609 WaveguideCleaningKitKITLWCC LWCCStart-upKit*58450 Kit,AdapterSyringe,LWCC

*includes FO-400-SMA1M (two), 58006, 500291, 501609

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Samplevolumesassmallas2microliters

SpectroPipetter™

SpectroPipetter™is the smallest and easiest to use microliter spectroscopic sample cell on the market. Withitsespeciallylowsamplevolumeofonly2microliters,theSpectroPipetterisideallysuitedforproteinandDNAanalysis.Initsoutwarddesign,theSpectroPipetterresemblesastandardlaboratorypipette.Fluidistakenupandexpelledbymeansofamanualplunger,justasinastandardpipette.Theplungerismadeofanopticalfiberbundle,protectedbystainlesssteeltubing.

Inadditiontomovingthesampleinandoutofthesamplechamber,itdeliversandcollectsthelightusedforabsorbancemeasurements.Thisdesignallowseasy,quickandaccuratespectroscopicmeasurementsofsmallsamplevolumes.Twofiberopticcablesareusedtoconnectthe

SpectroPipettertoaspectrophotometer.Thelaunchcableisusedtocouplelightfromalightsourcetothesamplechamber,whichisdesignedusingwaveguideprinciples.Thereturnguidesthelightfromthesamplechambertoaspectrophotometer.TheSpectroPipetterisideallysuitedformeasurementswithWPI'sTIDAS IIspectrophotometersystem.Further,modularsamplesystemscanbeassembledusingWPI’sPDA(TIDAS I)orCCDbasedspectrometermodulesandWPI’srangeofUV/VISlightsources(D2H,D2Lite™,andFO-6000).WerecommendtheSPT-2forTIDASII-andTIDASI-basedsystemsandtheSPTforS2000-orSpectraUSB-basedsystems.

Inadditiontothestandardaccessories,theoptionalUltrasonic Cleaning Kitishighlyrecommendedforkeepingthesamplechambercleanandforremovinganyresidualsamplefluidordyefromthesamplecell.Thekitcontainsanultrasoniccleaningbathforthetipmodule,aspecialstandforplacingtheSpectroPipetterintheultrasonicbathandabottleofconcentratedcleaningsolution.Abuilt-intimerprovidesa20-secondcleaningcyclewithahighintensityultrasonicwaveeachtimeitisactivated.

ReferencesS.X.Liu,J.P.Fabisiak,V.A.Tyurin,G.G.Borisenko,B.R.Pitt,J.S.Lazo,V.E.Kagan,“ReconstitutionofApo-SuperoxideDismutasebyNitricOxide-InducedCopperTransferfromMetallothioneins”,Chemical Research in Toxicology,2000.

S.Aleman,et al.,“Long-termeffectsofantiretroviralcombinationtherapyonHIVtype1DNAlevels”(1999)Aids Research And Human Retroviruses15:14.

T.M.Redmond,S.Yu,E.Lee,D.Bok,D.Hamasaki,N.Chen,P.Goletz,J.X.Ma,R.K.Crouch,K.Pfeifer,“Rpe65isnecessaryforproductionof11-cis-vitaminAintheretinalvisualcycle”,Nature genetics,vol.20,(1998),344-351.

U.S

.Pat

ents

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SPECTROPIPETTER SPECIFICATIONS SPT SPT-2WAVELENGTHRANGE(withTIDAS-2) 230-850nm 230-850nmSAMPLEVOLUME 2microliter 2microliterLIGHTPATHLENGTHOFCELL 1.0cm 1.0cmTEMPERATURERANGE 4to99°C 4to99°COPTICALFIBERLENGTH 1.3m 1.3mCOREDIAMETEROFLAUNCHFIBER(S) 400µm 200µmbundle(3)COREDIAMETEROFRETURNFIBER 100µm 200µm

Microliter Sampling

Pistonispartoffiberopticpathway

Quartzguide

Protectivemetalsheath

Cellopening

MirrorAfterpassingthroughsample,reflectedlight

returnstothespectrometer

SpectroPipetterIIandUltrasonicCleaningKit

Sample



SPT SpectroPipetterSPT-2 SpectroPipetter-II—OptimalUVEfficiencySPT-UCK SpectroPipetter&UltrasonicCleaningKitSPT-2-UCK SpectroPipetter-II&UltrasonicCleaningKit

OPTIONAL ACCESSORIES15807 CleaningSolutionConcentrate(125mL)UCK UltrasonicCleaningKit



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Microliter Sampling

DipTip™isaminiaturetransmissionprobeformicroliterspectroscopicsampling.DipTip’stipdiameterisonly1.5mm—thesizeofa17-gaugeneedle.ItwillfitintoallmicrocentrifugetubesonthemarketandisaveryusefultoolformeasuringproteinandDNAsamples.Thediminutivedippingprobecanalsobeusedforadissolutionsystem.ThankstoWPI'sproprietaryopticaldesign,DipTipisnotonlyfourtofivetimessmallerindiameterthananyotherproductonthemarket,butalsocosts50%less.Togetherwithourfiberoptic-basedspectrophotometers(Tidas II)orspectrometermodules(Tidas I)orCCD(SpectraUSB)andlightsources(D2H,D2Lite™,andFO-6000),microlitersamplescanbeanalyzedverycosteffectively.

Idealformulti-channelapplications.

World’s Smallest Fiber Optic

Dipping Probe

forUV/Vis

Spectroscopy

forUV/Vis

Spectroscopy

DIPTIP SPECIFICATIONS DIP-UV DIP-NIR DIP-UV-SR

TipDiameter 1.5mm 1.5mm 1.5mm

LightPathlength 2,5,10mm 2,5,10mm 2,5,10mm

WavelengthRange(nm) 230-1000 350-1000 200-1000

SampleVolumeRequired 20-50µL 20-50µL 20-50µL

DistanceFromTiptoUpperEdgeofSampleWindow 7mm 7mm 7mm

TotalLength(includinghandle) 18cm 18cm 18cm

FiberLength 1.5m 1.5m 1.5m

FiberOpticConnection SMA905 SMA905 SMA905

LaunchFibers(2)NA=0.22 400µm 400µm 400µm

ReturnFiber(1)NA=0.22 400µm 400µm 400µm

10mmlightpathlength

5mmlightpathlength

2mmlightpathlength



DIP-UVx DipTip™forUV/VISSpectroscopy(2,5,and10mmpath)DIP-NIRx DipTip™forVIS/NIRSpectroscopy(2,5,and10mmpath)DIP-UV-SRx DipTip™forUV/VISwithsolarresistantfibers(2,5,and10mmpath)UCK UltrasonicCleaningKit

Specifypathlengthwhenordering:X=2,5or10

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CuvetteHolderfor10cmCuvettesTheCUV-UV-10isacuvetteholderfor10cmcuvettesandcouplestoalightsourceandaspectrometerviaopticalfibers.Thecuvetteholdercanbeusedforabsorbanceandtransmissionmeasurementsofaqueoussolutions.Eachcuvetteholderassemblycomeswithablackcover,whichcanbeusedtoeliminateambientlight.WPI’sCUV2071-1,a10cmcuvettewithasamplevolume

of28mLcanbeusedtoperformmeasurementswitha10cmpathlength.

WPI’scuvetteholderscanbeconnectedviaopticalfiberstoourlightsourcesanddetectorstobuildmodularabsorbance/transmissionorfluorescencedetectionsystem.TheyareoptimizedforUV/VIS/NIRapplicationsinthe200-nmto2µmrange.Quartz,glassorplasticcuvettesofvarioussizescanbeusedwiththemtoanalyzeyoursamplesolutions.Somekeydesignfeaturesare:●Apairofadjustable5-mmdiameterf/2quartzfiberopticcollimators●Springloadedballplungersforprecisecuvettepositioning●Built-in1/4-inchfilterslot●Temperaturestabilizationwithawaterbathviainternalliquidchannelsinthecuvetteholderbase●SMA905terminationsforfiberopticcoupling

CuvetteHolderfor1cmCuvettesTheCUV-UVisacuvetteholderfor1cmcuvettesandcouplestoalightsourceandspectrometerviaopticalfibers.WPI’squartzandglasscuvettescanbeconvenientlyusedtomeasureabsorbencieswithsamplevolumesrangingfrom0.2-mL(CUV2034-1)to3.5-mL(CUV2012-1).Further,flowthroughsystems

canbeassembledwhenusingWPI’scuvetteflowcellsCUV2061-1orCUV2062-1.Ablackcuvettecoverisavailabletoeliminateambientlightandtoblockthelightpath,whentakingdarkcurrentmeasurements.

4-wayCuvetteHolderfor1cmCuvettesTheCUV-ALL4-wayCuvetteHolderfor1-cmpathlengthcuvettesisequippedwithfiberopticcouplings,whichcoupletoopticalfiberstoeitherreadorilluminatethesample.Whenusedwithspectrometersandlightsources,CUV-ALLCuvetteHolderscanmeasureabsorbance,fluorescence,scatteringoranycombinationoftheseopticalphenomena.Thecuvetteholdercanbeusedfor“straight-through”measurements,

aswellasforscatteringorfluorescencemeasurements.TheCUV-ALLCuvetteHolderacceptsallWPI’s1cmcuvettes;adjustablespring-loadedpistonsholdthecuvetteagainsttworeferencesurfaces.Theassemblyalsohasabuilt-in1/4-inchfilterslot,especiallyconvenientfortailoring(withbandpassfilters)excitationwavelengthsinfluorescencemeasurements.

CuvetteHolderforFluorescenceApplicationsTheCUV-UV-FLisafluorescenceoptionoftheCUV-ALL,availablewithtwo,insteadoffourcollimators,toallowperpendicularsampleexcitationanddetection.Toincreasetheefficiency,mirroredscrewplugs(CUV-MSP)areavailabletoreflectexcitationandemissionlightbackintothesamplecell.

Cuvette Holders & Accessories

● Continuouslyvariablewavelengthinonefilter● Substantialeconomytousingfixedwavelengthfilters● Rugged,durable,andeasilycleanable● UVandVISversionsavailable● Especiallyusefulforfluorescenceapplications

WPI’suniquehighpassandlowpasslinearvariablefilterslidesvarytheirtransitionwavelengthalongtheirlengths.Aspecificwavelengthcanbeselectedbysimplyadjustingthefiltertotheappropriatelinearposition.

Asinglelinearvariablehighpassfilter(#502126)blocks98.8%oflightbelowandtransmits90%lightaboveitsselectabletransitionwavelength.Asinglelinearvariablelowpassfilter(#502127)passeslightat88%uptoitstransitionwavelengthandthenblocks98.8%.Whenaligningtwoidenticalhighpassorlowpassfilters,theopticaldensityoftheblockedwavelengthincreasesto99.96%,butthetransmissionisreducedtoapproximately80%.

Abandpassfilter(#502111)withadjustablecenterwavelengthandbandpasscanbeassembledbycombiningalowpassandahighpasslinearvariablefilterslide.Withthiscombination,centerwavelengthandtransmissionbandwidthcanbeuserselected.

AccessoriesAllfiltersarehousedinaslidecarrierandcanbeeasilyinsertedineitherainlinefilterholder(#502114)orcuvetteholder(#CUV-ALL).Anopticaladapter(#502112)isavailablefortheCUV-ALLcuvetteholdertoclamptheslidecarrierintoplaceandsetthefilterposition.AcuvetteshapedPTFEblock(#502113)canbeusedtoredirectexcitationlight90degreesfromtheexcitationportintotheemissionportofafluorescencecuvetteholder(CUV-ALLorCUV-UV-FL)tomonitorandadjustfilterperformanceduringtheexperimentalsetup.

Linear Variable Filters



CUV-UV CuvetteholderforUV/VIS/NIR,1cm501603 BlackCuvetteCover(optional)

CUV-ALL 4waycuvetteholder501603 BlackCuvetteCover(optional)

CUV-UV-FL FluorescenceCuvetteHolder501603 BlackCuvetteCover(optional)CUV-MSP Mirroredscrewplugs

Thefilterslistedbelowhavea300-750nmwavelengthrange.WealsoofferaUV(230-500-nm)versionofeachfilter.Callforassistance.

502126 Single-filter Slide for High-pass Applications502127 Single-filter Slide for Low-pass Applications502128 Double-filter Slide for High-pass Applications502129 Double-filter Slide for Low-pass Applications502111 Adjustable Bandpass Linear Variable Filter502112 Adapter for Cuvette Holder502113 Cuvette-shaped PTFE Block for Redirecting Excitation Energy502114 In-line Filter Holder for Linear Variable Filters502115 Kit consisting of 502111, 502112, 502113 and 502114

CUV-UV-10 CuvetteholderforUV/VIS/NIR,10cm $



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cence and flow) are shipped individually pack-aged, glass cuvettes are shipped in packages of 10 cuvettes. These economic Quartz and Glass cuvettes are ideal for precision measurements, because of their high quality materials used and their low manufacturing tolerances. Typical

transmission curves of glass and synthetic quartz cuvettes are shown in Fig. 1 (cuvettes were empty, thickness 1.25 mm x 2, including surface reflections, measured with a TIDAS II against air as reference).

A complete transmission spectrum from 190 nm to 4 mm is shown in Fig. 2 (cuvettes were empty, thickness 1.25 mm x 2, including surface reflections).

Quartz and optical glass cuvettes for spectrophotometry and fluorometry● High Quality Glass Cuvettes for $3/cuvette● High Quality Synthetic Quartz Cuvettes for $36/cuvette● Optical Glass: 350 – 2500 nm ● Synthetic Quartz: 185 – 2500 nm

WPI’s glass and synthetic quartz cuvettes are ideal for UV/VIS/NIR absorbance or fluorescence experiments. The cuvettes are manufactured by heat fusion into a single unit. This adhesive-free technology eliminates any solvent interference. WPI offers two window materials, fused quartz and glass. Synthetic quartz can be used in deep UV applications and is recommended for fluorescent applications, as it does not exhibit background fluorescence. Quartz cuvettes (absorbance, fluores-

200 400 600 800 1000

0

20

40

60

80

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Synthetic Quartz GlassT

ran

smis

sion

[%]

Wavelength [nm]

TEChniCaL ChaRaCTERiSTiCS

Cuvette Material Spectral Range Transmission Difference (>80%) Between Different CuvettesOptical Glass 350 – 2500 nm Less than 1%Synthetic Quartz 200 – 2500 nm Less than 1%

Fig. 1—Transmission curves of Glass and Synthetic Quartz Cuvettes

Fig. 2—Complete transmission curves of Glass and Synthetic Quartz CuvettesStyle a Style B Style C

Style D Style E * Style F * Style G

Useful accessories for cuvettes are the ultrasonic cleaner (PUC) on page

163, and the Cleaning Solution Concentrate (15807) on page 254.


World Precision instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: [email protected] • Internet: www.wpiinc.com

CaTaLOG # STYLE MaTERiaL WinDOWS PaTh DiMEnSiOnS VOLUME BEaM WiDTh [mm] [mm] [mL] [mm] aBSORBanCE CUV2011-1 B Quartz 2 5 7.4x12.4x45 1.7 10CUV2012-1 C Quartz 2 10 12.4x12.4x45 3.5 10CUV1022-10 C Optical Glass 2 10 12.4x12.4x45 3.5 10CUV2031-1 D Quartz 2 10 12.4x12.4x45 1 4CUV2032-1 D Quartz 2 10 12.4x12.4x45 0.75 3CUV2033-1 D Quartz 2 10 12.4x12.4x45 0.5 2CUV2034-1 D Quartz 2 10 12.4x12.4x45 0.2 0.5-1

FLUORESCEnCE CUV2051-1 A Quartz 4 10 12.4x12.4x45 3.8 10CUV2052-1 A Quartz 4 10 12.4x12.4x45 1 4

FLOW CELLS CUV2061-1 E Quartz 2 10 12.4x12.4x45 0.07 3CUV2062-1 F Quartz 2 10 12.4x12.4x45 0.48 4x12CUV2071-1 G Quartz 2 100 102.5 x d22 28 19

* Distance between base of cell and center of sample chamber: Style E, z = 16 mm; Style F, z = 15 mm.

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reflecting materials. Light is guided via six fibers with a core diameter of 200 µm from a light source to the sample. Reflected light is guided via a seventh fiber with a core diameter of 200‑µm to a light detector. The REF‑UV can be used with WPI’s fiber optic‑based spectrophotometer (TIDAS II) or spectrometer modules (TIDAS I, S2000 series or SpectraUSB) and light sources (D2H, D2Lite™, and FO-6000).

The TR-600 is a high efficiency transmission probe for dipping and online UV/VIS absorbance measurements in liquids. The probe end is dipped into a sample solution to perform a measurement. Launch and return fibers have a 600 mm core diameter, as well as the probe has a remarkable transmission (20% at 260 nm and over 30 % at 700 nm). This makes the probe ideal as a dipping probe for WPI’s TIDAS I and TIDAS-II as well as the S2000 and SpectraUSB series systems. Additionally, the probe can be conveniently used with spectrophotometers that required fiber optic adapters (e.g., Varian or HP). The probe works in the range of 200 nm to 1000 nm range. The optical pathlength can be selected between 2, 5 and 10 mm when purchased. Probe diam: 6.35 mm. Shaft length: 152 mm.

REF-UVREF-UV

TR-600TR-600

13395

13370

CC-3-UV

Reflectance Probes The REF-UV is a standard reflection probe to observe spectral information of diffuse or direct

The TR-UV is a transmission probe, which can be used for online absorbance measurements in liquids. Six fibers with a core diameter of 200‑µm are used to transport light from a light source to the sample cell, one 200‑µm fiber is used to couple light from the sample cell back into the spectrometer. The probe works in the

range of 200 nm to 1000 nm range. The optical pathlength can be selected between 2, 5 and 10 mm when purchased. The TR‑UV can be used efficiently with WPI’s TIDAS I high performance

spectrometer module and a D2H or FO-6000 light source or WPI’s CCD‑based spectrometer modules (SpectraUSB and S2000) and a D2H, D2Lite™ or FO-6000 light source. TR-UVTR-UV

Transmission Probes


UK: Tel: 01438‑880025 • Fax: 01438‑880026 • wpiuk@wpi‑europe.com Germany: Tel: 030‑6188845 • Fax: 030‑6188670 • wpide@wpi‑europe.com

TR-UV Transmission probe with 1 tip (2, 5, 10) TR-TIP-xx Replacement Tip (specify 2, 5 or 10 mm)

OPTIONAL ACCESSORIES13395 SMA Bulkhead Feedthru connector/coupler, D‑hole 13370 SMA half‑length Bulkhead coupler/connector CC-3-UV Cosine Corrector

TR600-xx Transmission probe with 1 tip (2, 5, 10 or 20 mm) TR600TIP-xx Replacement Tip (specify 2, 5, 10 or 20 mm)

REF-UV Reflectance Probe



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UV/VIS

Features●BroadUV/Visspectralrange●Laserdam-ageresistant●Highcoretocladratios●Broadtemperaturerange●Bio-compatiblematerials●Radiationresistant●SterilizablebyETOandgammaradiation●HighertransmissionthanPCSbetween180-nmand300nm

Properties●Multimode•Puresilicacore•Numericalaper-

ture:0.22±0.02(standard)●Standardprooftest:70kpsi•Minimumbend

radius:100xcladradius(momentary),600xcladradius(longterm)

Anti-SolarizationThetransmissionofconventionalUV-enhancedsilica/silicafiberdecreasesrapidlyatwavelengthsbelow240nmwhenexposedtohighintensitiesofadeuteriumlamp.Thiseffectiscalled“UV-solarization”andresultsfromthegenerationofcolorcentersinthefibermaterial.Thelifetime

UV-enhanced fiber optic cables

Transmission/m 99%

99.9%

AttenuationdB/km

1000—

100—

10—

1—

200 300 400 500 600 700 800 900 1000 1100Wavelength, nm

ofsuchafiber,definedbythe1/ereductionintransmissionat240nm,isnormallylessthan200minutes.ThiseffectrendersthemunsuitableforUVspectroscopybelow240nm.

Anti-solarizationfiberssuitablefordeepUVspectroscopysolvethisproblem.Thesefibersstabilizetolessthan5%degradationoveraperiodof20,000hoursafteraninitialtransmission“burn-in”lossoflessthan25%.Additionally,thisanti-solarizationcharacteristicwillnotdegradeovertime.

Over20,000hourswithlessthan5%

degradation!

Bifurcated Fiber Optic AssembliesUsetocombinesimilarintensitylightfromdifferingsourcesortosplitalightsourceintotwofibers.Forusewithadualspectrometerasareference.

UV/VIS

BIFURCATED ASSEMBLY SPECIFICATIONS

Length 1meter

Connectors SMA

Wavelengthranges 200-1100nm

CableType UV-enhanced

WPI can build custom fiber optic assemblies for many UV/VIS/NIR applications. Call for more information.



BIF22 Splitorcombinesimilarintensities(200/200)BIF44 Splitorcombinesimilarintensities(400/400)BIF41 CombineUV(400)+VIS(100) BIF62 CombineUV(600)+VIS(200) BIF66 SplitorCombineSimilarIntensities(600/600)

UV-ENHANCED FIBER OPTIC CABLES, 230 – 1000 NMFO-50-SMA1M FiberOpticCable,1m,SMA,50µmCore,UV-Enhanced FO-50-SMA FiberOpticCable,3m,SMA,50µmCore,UV-Enhanced FO-100-SMA1M FiberOpticCable,1m,SMA,100µmCore,UV-Enhanced FO-100-SMA FiberOpticCable,3m,SMA,100µmCore,UV-Enhanced FO-200-SMA1M FiberOpticCable,1m,SMA,200µmCore,UV-Enhanced FO-200-SMA FiberOpticCable,3m,SMA,200µmCore,UV-Enhanced FO-400-SMA1M FiberOpticCable,1m,SMA,400µmCore,UV-Enhanced FO-400-SMA FiberOpticCable,3m,SMA,400µmCore,UV-Enhanced FO-400SMA/ST FiberOpticcable,1m,SMA/STconnector,400µmcore,UV-Enhanced FO-600-SMA1M FiberOpticCable,1m,SMA,600µmCore,UV-Enhanced FO-600-SMA FiberOpticCable,3m,SMA,600µmCore,UV-Enhanced FO-1000-SMA1M FiberOpticCable,1m,SMA,1000µmCore,UV-Enhanced FO-1000-SMA FiberOpticCable,3m,SMA,1000µmCore,UV-Enhanced

ANTI SOLARIZATION FIBER OPTIC CABLES, 190 – 1000 NMFO-200AS-SMA FiberOpticCable,1m,SMA,200µmCore,Anti-Solarization FO-400AS-SMA FiberOpticCable,1m,SMA,400µmCore,Anti-Solarization FO-600AS-SMA FiberOpticCable,1m,SMA,600µmCore,Anti-Solarization

PLASTIC FIBER OPTIC CABLES (NON UV), 400 TO 1000 NMFOP1-SMA PlasticFiberOpticCable,SMAconnectors,1mmx2m FOP1-SMA/ST PlasticFiberOpticCable,ST/SMAconnectors,1mmx2m FOP1-ST PlasticFiberOpticCable,STconnectors,1mmx2m

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D2HDeuteriumhalogenlightsourcewithintegratedTTLshutterTheD2HisacombineddeuteriumandhalogenlightsourceforUV/VISandNIRapplications.ThislightsourceisideallysuitedtoworkwithWPI’sspectrometermodulesandsamplecells.ItsuppliesacontinuousspectrumintheUV,VISandNIRrangefrom215nmto1700nm.TheD2Hisequippedwithanintegratedelectricalshutter,whichcanbecontrolledbyaswitchoraTTLsignal.FordeepUVapplications(190-nm),theD2H-2shouldbeused.

Thisnewminiaturetungstenlightsourcehasbeendevelopedforhighprecisionportableandlow-powerspectroscopyapplications.Itsadvantageliesinitshighlightpoweroutput,itseffectivecolortemperatureof6000Kanditsexceptionallylowdriftbelow0.5mAU/h.TheFO-6000isalightsourcefortheextendedvisiblepartofthelightspectrum(380nm–1700nm).IthasaSMAtypeoutputconnector.ShutterandlampcanbeoperatedviaTTLexternaltriggering.Thislightsourceoffersawideassortmentoffieldapplicationsinanalyticalchemistryaswellasenvironmentalandlifescience.

Asignificantproblemwithtungstenlightbulbsistheirinherentlowlightoutputatwavelengthsbelow430nm.TheFO-6000wasdevelopedtoovercomethislimitation.Thelightintensityofatungstenlightbulb(2760K)dropsbelow10%at420nmwavelength.However,usingFO-6000,thelightintensitydropsbelow10%at370nm,wheretheintensityoftheconventionaltungstenlightbulbisatapproximately2%relativelightoutput.TheinherentlownoiseoftheFO-6000makesitparticularlysuitableforlow-noisedetectionsystems.

FO-6000FILTTheFO-6000FILTinlinefilterholderdirectlyattachestotheFO-6000lightsource.Thisallowsavirtuallightlossfreeinsertionofopticalfilterswithouterdiametersfrom8to25.4mmandthicknessrangingfrom2to10mmintothelightpathoftheFO-6000.Withthisfilterholderandanopticalfilter,ahighlystablemonochromaticlightsourcecanbeassembled.

FO-6000™

Highcolortemperaturetungstenlightsource

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UV Safety

GogglesGogglesfitoverregularglasses



FO-6000 FiberOpticLightSourceFO-6000FILT InlineFilterHolderAdapterforFO-6000800120 ReplacementLampforFO-6000

D2H DeuteriumHalogenLightSource(215nm–1700nm)D2H-2 DeuteriumHalogenLightSource(190nm–1700nm)D2H-HB HalogenreplacementlampD2H-DB Deuteriumreplacementlamp(>215nm)D2H-HBER Deuteriumreplacementlamp(>190nm)

13410 UVSafetyGoggles



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LED-LiteModularLEDLightsourcewithexchangeableLEDsTheLED-liteisapowersupplyforWPI’sELSLEDmodulesformonochromaticlightexcitation.EachELSmodulehasanSMAbulkheadfittingandallowsdirectattachmentofSMAterminatedfibers.

Deuteriumlightsourcesolutionforhand-held

spectroscopy

Thisnewminiaturedeuteriumlightsourcehasbeendevelopedforportableandlow-powerspectroscopyapplications.Itsadvantageliesinfastandconvenientsinglemeasurementcyclesanditsshortwarminguptime.D2LiteisacompleteUV-VISlightsource(200nm–1100nm)withshine-througharrangementofadeuteriumandatungstenlamp,shutter,opticalsystemwithSMAconnectorandpowersupplyforthetwolamps.BothlampsandshuttercanbeoperatedviaTTLexternaltriggering.Thislightsourceoffersawideassortmentoffieldapplicationsinenvironmentalandlifesciences.

ToorderELS,usewavelengthassuffixtopartnumber(e.g.ELS-430).

ELS Spectral Distributions Spectral Line Estimated Color λmax Half Width Output

UV 370nm 12nm 85µW Blue 430nm 65nm 15µW Blue 450nm 70nm 119µW Blue 470nm 20nm 140µW Blue-Green 495nm 35nm 227µW Green 525nm 40nm 80µW Yellow 590nm 13nm 60µW Orange 623nm 15nm 114µW Red 660nm 35nm 275µW

Estimated output is after light has passed through a 1 mm fiber.

ELSmodule

LIGHT SOURCE SPECIFICATIONS D2LITE D2H / D2H-2 FO-6000APPLICATION UV/VIS/NIR UV/VIS/NIR VIS/NIR

SPECTRALRANGE 200—1700nm 215/190—1700nm 380—1700nm

DEUTERIUMLAMPLIFE 1000hr 1000hr NA

TUNGSTEN/HALOGENLAMPLIFE 2000hr 1000hr 3000*hr

STABILITY <1mAU/h <0.5mAU/h <0.5mAU/h

POWERCONSUMPTION 6W 100W 6W

POWERREQUIREMENTS 12Vdc/0.5A 110/240V,50-60Hz,1A 12VDC/1A

SHUTTER/TTLTRIGGER Yes Yes Yes

MAX.FIBEROUTPUT 1000mm 1000mm 1000mm

CONNECTIONS SMA SMA SMA

SHIPPINGWEIGHT 1.0lb(0.4kg) 13.2lb(6kg) 1.3lb(0.6kg)

DIMENSIONS(W/H/L) 2.7x2.6x6.7 5.3x5.9x12.6 4.8x2.8x7.5 (7x6.5x17) (13.5x15x32) (12x7x19)

*Lamp life is dependent upon internal power settings.

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D2LITE-Y D2LiteDeuteriumLightSource(120V,USplug)D2LITE-B D2LiteDeuteriumLightSource(240V,UKplug)D2LITE-Z D2LiteDeuteriumLightSource(240V,Europlug)D2LITE-R ReplacementLamp

LED-LITE ELSPowerSupply(requires ELS module) Includes transformer and AC adapter.

Specifylinevoltage

ELS-xxx ExternalLightSourceModule (specify wavelength)ELS-370 ELSModule (370 nm)300051 FiberOpticCollimator(SMA)300052 FiberOpticCollimator(ST)

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In-Line Fiber Optic Filter HolderThisveryusefulinstrumentallowstheinsertionofopticalfilterswithinafiberopticpathway.Theconnectorsofthefilterholderassemblyarecom-patiblewithWPI’srangeoffiberopticjumpercablesandcanbecoupledusingSMAorSTconnectors.

Filterswithouterdiametersfrom8to25.4mmandthick-nessesfrom2to10mmcanbeaccomodated.Thedesignlimitslateralandaxialmovementofthefilterwhensecuredintheholder.

Twofiberopticcollimatorsareinternallymountedintheholdersoastopasscollimatedlightthroughthefilterandthenrefocusthefilteredlightintotheapertureoftheoutputfiber.Spectralrangewillbelargelylimitedbythebandpassoftheopticalfibers(fromUVtonearIRusingWPIUV-enhancedcables).

WPI’sFiberOpticCollimatorcanbeusedforbothcollimatingalightbeamemittedbyanopticalfiberorcouplinglightfromacollimatedlightbeamintoanopticalfiber.Thenumericalapertureofthecollimatorisoptimizedformaximumcouplingefficiencyintotypicalfusedsilicafibers.Thecollimatorcan,forexample,beusedtoguideaparallellightbeamthroughasamplecuvetteoranopticalfilterwithvirtuallynoopticallosses.Inthisapplication,onecollimatorcollimatesthelightintoaparallelbeam5mmindiameter,enablingittopassalongdistancewithoutlosingtheenergy.Afterthelightpassesthesamplemedia,asecondcollimatorcanbeusedtocollectthebeamintothereceivingfiber.Auniquedesignfeatureofthiscollimatoristhatthedistancebetweenthelensandtheopticalfibercanbeeasilyadjusted.Thispermitsittobeusedasafocusingdeviceorforfine-tuningthecolorbalancewhencouplinglightfromalightsourceintoafiber.

Fiber Optic Collimator

COLLIMATOR SPECIFICATIONSLENSDIAMETER 5mm

LENSFOCALDISTANCE 10mm

LENSMATERIAL Ultravioletgradesyntheticfusedsilica(KU-1)

WAVELENGTHRANGE 170nm-2µm

MOUNTINGTHREADS 3/8-24UNF

DIVERGENCE <0.1radfor1mmcorefiber

FIBERCONNECTORINTERFACE SMAorST

PeroxiLuminol HydrogenPeroxideDeterminationKitBasedonPeroxidase-CatalyzedLuminolChemiluminescence

HydrogenPeroxideisproducedinbiologicalsystemsbycontrolledpathwaysatlowconcentrationsthatimpactoncellsignaling.Athigherconcentrationsinflammatorycellsproducelocalintenseamountsofthisoxidanttokillpathogens.Inthedevelopmentofhumandiseaseuncontrolledformationofhydrogenperoxidefromthemitochondrialrespiratorychainandenzymessuchasxanthineoxidasecanoccur(Prof.VictorDarley-Usmar,UniversityofAlabama,personalcommunication).HydrogenperoxideconcentrationsatµMlevelelicitabroadspectrumofcellularresponsesrangingfrommitogenicgrowthstimulationtoapoptosisandnecrosis.PeroxiLuminolisahydrogenperoxidechemiluminescencebioassaykitdevelopedbyWPItoprovideanaccurateanalyticaltoolbywhichtomeasurehydrogenperoxideformationinthelivingsystem.ThekitisdesignedtocomplimentWPI’snewrangeofhydrogenperoxideelectrochemicalsensors.Whencombinedtogetherthesedetectionmethodswillgreatlyaidhydrogenperoxideresearch.

Assay Principle: Horseradishperoxidase(HRP)reactswithhydrogenperoxidetoformreactiveCompoundIandCompoundII,whichinturnoxidizeluminolwithconcomitantproductionofchemiluminescence(seereactionschemebelow).ThePeroxiLuminolKithasbeenoptimizedsothathydrogenperoxideconcentrationislinearlyproportionaltoluminescenceintensity.Ourformulationassuresthatluminescenceintensitydoesn’tdecaytooquicklysothatmeasurementcanbemadeaccuratelyandprecisely.Theslowdecayratealsoallowsluminescencemeasurementwithsimpleluminometersthatmaynotincludeinjection/mixingdevices.ThefigureaboveshowshydrogenperoxidestandardsasassayedbytheHRP-catalyzedluminescencemethod.Benefits

•Determinehydrogenperoxideconcentrationinminutes.•Simpleandextensivelytestedmethodwithanincreasedsensitivity.•Morestablesignals.•Inexpensivemeasurementinstrument.

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56200 In-LineFiberOpticFilterHolder(SMA)56300 In-LineFiberOpticFilterHolder(ST)

300051 FiberOpticCollimator(SMA)300052 FiberOpticCollimator(ST)

Note:This kit is designed to assay hydrogen peroxide in cell cultures. Samples with high hemoglobin content and/or metal content may not be suitable for assay. Due to continued development, the kit format and performance parameters may be changed without a notice. Contact wPI for further information.

PEROXYLUMINOL Hydrogen Peroxide Determination Kit

Thiskitprovidesmaterialfor100assaysincuvettesortesttubes(assayvolume:1mL/assay).



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Glutathione(GSH),atripeptideantioxidant,playsmultiplebiologicalfunctions.Itisinvolvedindetoxificationofharmfulmolecules,suchasreactiveoxygenspecies(i.e.,hydrogenperoxideandhydroperoxides)throughglutathioneperoxidaes,andxenobioticsthroughglutathionetransferase.Itisalsoinvolvedinsignaltransduction,geneexpression,apoptosisandinteractionwithnitricoxide.Glutathioneantioxidantstatusisfrequentlymeasuredinphysiologyandpathophysiology.

Assayprincipal:GSHreactswithEllman’sreagent(5,5’-dithiobis(nitrobenzoicacid)orDTNB)toproduceachromophoreTNBwithmaximalabsorbanceat412nmandoxidizedglutathioneGSSG.Tietzeformulatedanenzymaticmethod

toregenerateGSHusingGlutathioneReducatseandNADPH,toprovidespecificityandimprovedsensitivityfortheassay.Thereactionmechanismisdepictedschematicallyinthefollowingfigure.Sinceglutathionereductaseisused,theamountofglutathionemeasuredrepresentsthesumofreducedandoxidizedglutathioneinthesample([GSH]t=[GSH]+2x[GSSG]).Therateofabsorbancechange(∆A412nm/min)ismadetobelinearfortheconvenienceandconsistenceofmeasurement,andislinearlyproportionaltothetotalconcentrationofGSH.Theconcentrationofanunknown(sample)isdeterminedbycalculatingfromthelinearequationgeneratedfromseveralstandardsofglutathione.

GSH ASSAY KIT QuantitativeDeterminationofGlutathioneUsingTietze’sEnzymaticRecyclingMethod

Other Benefits● Formulatedforconvenientandfastassayforbiologicalsamples—

3minutes/assayatroomtemperature.

● Usesimplespectrophotometersormicrotiterplatereaders.

● Nointerferencefromcysteineandmetalions.

● Wideassayrangefrom0.1µMto30µMinsample(dilutionisrequiredforsampleswithhigherGSHconcentration.)

● Kitincludesallrequiredreagentsandstandards.

Selective References1. SiesH.,“Glutathioneanditsroleincellularfunction”,Free Rad.

Biol. Med.(1999),27,916-921.

2. Griffith,O.W.,“Biologicalandpharmacologicregulationofmammalianglutathionesynthesis”,Free Rad. Biol. Med.(1999),27,922-935.

3. TietzeF.,“Enzymicmethodforquantitativedeterminationofnanogramamountsoftotalandoxidizedglutathione:applicationstomammalianbloodandothertissues”,Anal. Biochem.(1969),27,502-522.

NADPH GR

TNB GSTNB TNB(λ412nm) (λ412nm)

GSHt ↔ ∆TNB/min ↔ ∆A412nm/min

GRNADPH

GSHGSSG DTNBGSHt

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12 µM GSH

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24 µM GSH

30 µM GSHy = 0.0175x + 0.0096

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GSH-KITTotalGlutathioneDeterminationKit

50 cuvette assays/200 microplate well assays

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NITRALYZER™-II NitratetoNitriteReductionKitThiskitusesdisposablecopper-coatedcadmiumgranulestoconvertnitrateintonitriteinminutes,withconsistent~100%efficiency.ItiscompatiblewithbothWPI’sGRIESS REACTION NITRITE KITandISO-NOPelectrochemicalnitriteassaymethod.

Nitrateandnitriteareendproductsofshort-livednitricoxidein-vivo.Nitriteconcentrationcanbemeasuredbyanumberofwell-establishedmethods.Nitrateassayrequiresconversionintonitritebeforethemeasurement.Copper-coatedcadmiumisthemostpopularchemicalreduceramongresearchers.Alternativemethodsbasedonenzymaticreductionofnitraterequiremuchlongerreactiontimesandtheirconversionefficiencydecreasesathighnitrateconcentrations.Advantagesofcadmiumbasedmethodincludestabilityuponstorageandinvariabilitybetweendifferentlots.

Benefits● ~100%conversionefficiencyinminutes(upto100µMnitrate).

● Disposable–usedonlyoncetoyieldconsistentresults.

● Conversiontakesplaceatroomtemperature.

● CompatiblewithbothGRIESS REACTION NITRITE KITandISO-NOPelectrochemicalnitriteassaytechnique.

● Scalable–smallervolumesforhard-to-obtainbiologicalsamples(12µLminimumsamplevolume).

● Kitincludesallrequiredreagents,includingdeproteinizationreagents.

● ExtensivelytestedinWPI’slaboratory.

GRIESS REACTION NITRITE KITQuantitativeDeterminationofNitriteThiskitusesthestandardcolorimetricGriessReactiontomeasurenitriteconcentrationinbiologicalsamples.Whennitrateortotalnitrateplusnitrite(Nox)ismeasured,samplesmustfirstbetreatedbyNITRALYZER-IIKittoconvertallnitrateintonitrite.

SpectrophotometricquantificationofnitriteusingGriessreagentsissimple,reliableandcost-effective.Itiswidelyusedinnumerouslaboratoriesaroundtheworldandremainsthemethodofchoiceforsomeapplications.NoxassayrequiresreductiontonitritebeforeGriessReaction.NitrateconcentrationcanthenbedeterminedbycalculatingthedifferencebetweenNoxconcentrationandnitriteconcentration.

Benefits● Simple(fast)andreliable,suitableforlargenumberofsamples.

● Lowcost.

● 0.3µM–100µMassayrange(assumingthatnodeproteinizationisrequired).

● Kitincludesallrequiredreagents,includingdeproteinizationreagents.

● Assayatroomtemperature.

● Extensivelytestedinourlaboratory.

Spectrophotometer and semi-micro cuvettes are required for the assay (see Spectroscopy Section). Alternatively, a microtiter plate reader can be used for absorbance measurement.

Copperized Cadium Nitrate Reducer

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RNitrite Standards

Nitrate Standards

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GRNK GriessReactionNitriteKit,100-500Nitritetests

GRNK-II GRNK+NITRALYZER-II,100-500Noxtests

NITRALYZER II NitratetoNitriteReductionKit

Kit contains material for 100–500 conversions



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Product Index100042................................................................. 84121..........................................................................101300M................................................................... 3213024.................................................................. 17813025.................................................................. 17813142.................................................. 29,.30,.23813156-100......................................................... 17313157-100.......................................................... 17313158-100......................................................... 17313159-100......................................................... 17313160-100......................................................... 17313161-100.......................................................... 17313162-100.......................................................... 17313163-100.......................................................... 17313316........................................................ 167,.18513324.................................................................. 17513338.........................................................213,.21613347.................................................................. 17513370................................................................. 25813388................................................................. 17513395................................................................. 25813410................................................................. 26013451.................................................................. 1751350M................................................................... 3213555.................................................................. 1751358.................................................................... 17513620.................................................................. 17513685.............................................175,.229,.23113740........................................................163,.16813776.................................................................. 17513822-10........................................................... 17313854.................................................................. 17513962.......................................................229,.23114003.&.14003-G........................................... 11914011.................................................................. 17214012.................................................................. 17214034-40........................................................... 17314035-10........................................................... 17314036-15........................................................... 17314038-10........................................................... 17314039-10........................................................... 17314040-50........................................................... 17314041-60........................................................... 17314042-100......................................................... 17314043-20........................................................... 17314044-5.............................................................. 17314045-20........................................................... 17314047-10............................................................ 17314048-20........................................................... 17314051-100......................................................... 17314054-10........................................................... 17314055-2.............................................................. 17314057-10............................................................ 17314058-10........................................................... 17314059-2.............................................................. 17314061-60........................................................... 17314073-4.............................................................. 17714080.&.14080-G........................................... 13114081.&.14081-G............................................13214084.................................................................13814088................................................................. 17514089.&.14089-G...........................................14614090.&.14090-G...........................................14614091.&.14091-G............................................14614095.................................................................10514096.................................................................10514097..................................................................10614098.................................................................10414099.................................................................10514101...................................................................10514104..................................................................19514106..................................................................19514109.&.14109-G.............................................13314110.&.14110-G.............................................13314111.&.14111-G.............................................12214112.&.14112-G.............................................12214113.&.14113-G............................................. 11314114.&.14114-G............................................. 11314116.&.14116-G.............................................13814117.&.14117-G..............................................13814118.&.14118-G.............................................13814119.&.14119-G.............................................13814120..................................................................13814121...................................................................13814122.&.14122-G............................................. 11914124.&.14124-G.............................................12014125..................................................................12214126..................................................................12214127.&.14127-G.............................................122

14128.&.14128-G............................................. 13114129.&.14129-G.............................................14014130.&.14130-G.............................................13914131.&.14131-G.............................................14014132..................................................................13214133.&.14133-G.............................................13214134.&.14134-G.............................................14414135..................................................................14414136.&.14136-G.............................................14914137.&.14137-G.............................................13214139..................................................................14414140.&.14140-G.............................................10914141.&.14141-G.............................................10914142.&.14142-G.............................................10914177.&.14177-G............................................. 11914187..................................................................10614188..................................................................10614189..................................................................13814192.&.14192-G.............................................12814206..................................................................13414207..................................................................13414208..................................................................13014209.................................................................. 11314211.................................................................. 11114212..................................................................13014213.................................................................. 16114214.&.14214-G.............................................12514215.&.14215-G.............................................12514216.&.14216-G.............................................12614217.&.14217-G.............................................12614218.&.14218-G.............................................12414219.&.14219-G.............................................12414220.&.14220-G...........................................12514221.&.14221-G............................................12514224.&.14224-G...........................................12414225.&.14225-G...........................................12414226.&.14226-G........................................... 11014239..................................................................23414240.&.14240-G...........................................14014253.................................................................... 2914254.................................................................. 17514292.&.14292-G...........................................14514342..................................................................16314361.................................................................. 13114364..................................................................12014365..................................................................12014393.&.14393-G...........................................12314394.&.14394-G...........................................12314395.&.14395-G...........................................12514396.&.14396-G...........................................12514404..................................................................16314405..................................................................16314417-F............................................................... 21914444..................................................................20315590................................................................. 21815623..............................................175,.229,.23115624..............................................175,.229,.23115657.................................................................... 4615676..............................................175,.229,.2311571........................................................... 167,.18715807..................................................................25415810..................................................... 82,.83,.8415867...................................................... 233,.23815873................193,.196,.197,.200,.202,.20315905.&.15905-G...........................................12315906.&.15906-G...........................................12315908................................................................. 11115910.................................................................. 11315911..................................................................13815914.&.15914-G.............................................10915915.&.15915-G.............................................10915916.&.15916-G.............................................10915917.&.15917-G.............................................10915918.&.15918-G............................................. 11015920.&.15920-G........................................... 11615921.&.15921-G............................................ 11615922.&.15922-G...........................................12315923.&.15923-G...........................................12315924.&.15924-G...........................................12815926.&.15926-G...........................................13315934.....................................................................3115975.................................................................. 17515976.................................................................. 17515978..................................................................18318-Dec.................................................................2201965....................................................................1831966....................................................................1831967....................................................................1831B100-3..............................................................180

1B100-4..............................................................1801B100-6..............................................................1801B100F-3............................................................1801B100F-4............................................................1801B100F-6............................................................1801B120..................................................................1801B120-4..............................................................1801B120-6..............................................................1801B120F-3............................................................1801B120F-4............................................................1801B120F-6............................................................1801B150-3..............................................................1801B150-4..............................................................1801B150-6..............................................................1801B150F-3............................................................1801B150F-4............................................................1801B150F-6............................................................1801B200-4..............................................................1801B200F-4............................................................1801B200F-6............................................................1802026-10.............................................................. 1752478...................................................................... 322479...................................................................... 322505....................................................................1872541.........................................................................32547.........................................................................5260.........................................................................112851.................................................................... 1752932.................................................................... 1782933.................................................................... 1782935.................................................................... 1782B150F-4............................................................1822B150F-6............................................................182300033.............................................................. 238300035.............................................................. 230300040.............................................................. 175300051...............................................................262300052...............................................................262300102........................................................24,.175300276............................................................... 218300305................................................................ 853006.................................................................... 1753161..................................................................... 1753259.......................................................................153260................................................................... 2363294.................................................................... 1753301.................................................................... 1753302.................................................................... 1753316.................................................................... 2363414..........................................................................73417-10............................................................... 1753468.................................................................... 1783469.................................................................... 1783484.................................................................... 1783485.........................................................................73491.................................................................... 1753492.................................................................... 1753508.................................................................... 1753517..................................................................... 1753531...................................................................... 323551...................................................................... 323578...........................................................175,.1913579-20............................................................... 573669...................................................................... 573670.................................................................... 1753742-20.............................................................. 1733955...................................................................... 573960...................................................................... 573993...................................................................... 593B120F-4............................................................1823B120F-6............................................................18240500.................................................................233472-4AFD............................................................. 49472-4SV................................................................ 4947262................................................................... 4947263................................................................... 4947264................................................................... 4947265................................................................... 4947266................................................................... 4947700.................................................................... 4948000....................................................................3148014.....................................................................3148015.....................................................................3148025....................................................................3148200....................................................................3148300....................................................................314878................................................................... 2384879................................................................... 2384886....................................................................166

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119500087...............................................................137500088.&.500088-G.....................................137500092.&.500092-G..................................... 110500121................................................................163500126...............................................................164500128............................................................... 175500131...................................................... 175,.190500133...............................................................148500134...............................................................148500135...............................................................148500137.&.500137-G.......................................147500143...............................................................146500162.............................................................. 209500168................................................................. 59500177.&.500177-G....................................... 110500178.&.500178-G....................................... 110500184............................................................... 175500186............................................................... 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110500223.&.500223-G.....................................132500224.&.500224-G.....................................134500225.&.500225-G.....................................134500226.&.500226-G.....................................134500227.&.500227-G.....................................135500228.&.500228-G.....................................122500229...............................................................103500230..............................................................103500231...............................................................104500232...............................................................105500233...............................................................104500234...............................................................106500235...............................................................105500236.&.500236-G.....................................143500237.&.500237-G.....................................143500238.&.500238-G.....................................143500239-500248.............................................143500240..............................................................143500241...............................................................143500242...............................................................143500243...............................................................143500244...............................................................143500245...............................................................143500246..............................................................143500247...............................................................143500248..............................................................143500249...............................................................144500250..............................................................144500252............................................................... 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214500280..............................................................168500282..............................................................226500283..............................................................226500291...............................................................253500292..........................................................29,.30500299............................................................. 238500313...............................................................142500314...............................................................142500315...............................................................142500316...............................................................142500317...............................................................142500325...............................................................142500326...............................................................142500329..........................................................29,.30500330..............................................................190500335...............................................................103500336..............................................................103500337...............................................................103500338..............................................................103500339..............................................................104500340..............................................................104500341...............................................................104500342...............................................................104500343...............................................................137500344...............................................................137500345...............................................................137500346..............................................................137500347...............................................................137500348..............................................................145500348-500356.............................................144500349...............................................................144500350..............................................................144500351...............................................................144500352...............................................................144500353...............................................................144500354...............................................................144500355...............................................................144500356..............................................................144500358..............................................................147500362.&.500362-G.....................................147500363.&.500363-G..................................... 111500364.&.500364-G..................................... 111500365.&.500365-G..................................... 111500366.&.500366-G.....................................127500367.&.500367-G.....................................127500368.&.500368-G.....................................139500369.&.500369-G.....................................139500370...............................................................155500372...............................................................130500373............................................................... 115500374...............................................................130500376...............................................................130500377............................................................... 115500378.&.500378-G.....................................128500379.&.500379-G.....................................128500380.&.500380-G.....................................129500381............................................................... 131500388..............................................................157500389..............................................................157500390..............................................................157500391...............................................................157500392..............................................................157500393..............................................................157500394..............................................................157500395..............................................................157500396..............................................................157500397...............................................................159500421...............................................................159500444.&.500444-G.....................................129500445............................................................... 131500446.&.500446-G..................................... 118500448..............................................................148500449...............................................................148500450..............................................................147500451.&.500451-G...................................... 116500452.&.500452-G..................................... 116500453............................................................... 115500455............................................................... 115500456.&.500456-G..................................... 115500457............................................................... 116500458..............................................................149500460..............................................................149500462..............................................................147500475.....................................................200,.201

500476.....................................................200,.201500778.............................................................. 238500787................................................................. 53500788................................................................. 53500789................................................................. 53500811......................................................214,.215500812......................................................214,.215500813............................................................... 215500814......................................................214,.215500815............................................................... 215500816............................................................... 214500820-F........................................................... 219500821......................................................214,.215500822.............................................................. 215500823.............................................................. 215500825.............................................................. 214500826.............................................................. 214500827......................................................214,.215500828............................................211,.214,.215500871...............................................................204500876...............................................................234500877...............................................................234500890.............................................................. 172500895.............................................................. 172501090...............................................................157501200...............................................................149501201................................................................105501202...............................................................105501203...............................................................105501204...............................................................106501205...............................................................106501206...............................................................106501207...............................................................108501208...............................................................108501209...............................................................108501210................................................................108501211................................................................ 118501212................................................................ 115501213................................................................159501214................................................................159501215.&.501215-G........................................109501216.&.501216-G........................................ 110501217.&.501217-G......................................... 111501218.&.501218-G........................................128501219.&.501219-G........................................128501220.&.501220-G.......................................128501221.&.501221-G.......................................128501222.&.501222-G.......................................128501223.&.501223-G.......................................128501224.&.501224-G.......................................128501225.&.501225-G.......................................128501226.&.501226-G.......................................128501227.&.501227-G.......................................128501228.&.501228-G......................................128501229.&.501229-G.......................................128501230.&.501230-G......................................128501231.&.501231-G.......................................128501232............................................................... 119501233............................................................... 119501234.&.501234-G.......................................120501235...............................................................122501236.&.501236-G......................................122501237...............................................................122501238...............................................................122501239.&.501239-G...................................... 112501240.&.501240-G...................................... 118501241.&.501241-G....................................... 117501242.&.501242-G....................................... 117501243.&.501243-G....................................... 118501244.&.501244-G....................................... 110501245.&.501245-G....................................... 110501246.&.501246-G......................................134501247.&.501247-G.......................................143501248.&.501248-G......................................143501249...............................................................143501250...............................................................143501251................................................................143501252.&.501252-G.......................................125501253.&.501253-G.......................................125501254.&.501254-G.......................................125501255.&.501255-G.......................................125501256...............................................................129501259.&.501259-G......................................127501260.&.501260-G......................................127501261.&.501261-G.......................................126501262.&.501262-G.......................................126501263.&.501263-G.......................................124501264.&.501264-G.......................................124501265.&.501265-G....................................... 111501266.&.501266-G...................................... 111501267.&.501267-G....................................... 111501268.&.501268-G...................................... 111501269...............................................................147501273-501286...............................................160

501274................................................................160501275...............................................................160501277...............................................................160501278...............................................................160501279...............................................................160501280...............................................................160501281................................................................160501282...............................................................160501283...............................................................160501284...............................................................160501285...............................................................160501286...............................................................160501287.&.501287-G....................................... 118501288.&.501288-G...................................... 117501291.&.501291-G....................................... 116501292-501300...............................................158501293...............................................................158501294...............................................................158501295...............................................................158501296...............................................................158501297...............................................................158501298...............................................................158501299...............................................................158501300...............................................................158501301................................................................148501302...............................................................106501303............................................................... 116501304...............................................................142501312................................................................135501313................................................................148501314.&.501314-G........................................ 141501316................................................................148501317................................................................148501318................................................................148501319................................................................162501320.&.501321-G....................................... 116501321.&.501321-G.............................130,.169501322.&.501322-G.......................................129501324.&.501324-G....................................... 117501325.&.501325-G....................................... 118501326.&.501326-G....................................... 117501327.&.501327-G....................................... 141501328.&.501328-G...................................... 141501329............................................................... 141501331................................................................155501332...............................................................145501333...............................................................145501335...............................................................143501336...............................................................156501337...............................................................144501338...............................................................144501339...............................................................144501340-501349...............................................162501341................................................................162501342...............................................................162501343...............................................................162501344...............................................................162501345...............................................................162501346...............................................................162501347...............................................................162501348...............................................................162501349...............................................................162501352............................................................... 213501353............................................................... 213501367............................................................... 213501369............................................................... 213501370............................................................... 213501371................................................................ 213501372............................................................... 213501373............................................................... 213501375............................................................... 213501376............................................................... 213501377............................................................... 213501378............................................................... 213501379............................................................... 213501381................................................................ 213501601................................................................169501603.............................................................. 256501608................................................................. 64501609.................................................... 249,.253501610................................................................169501622........................................... 193,.194,.196501623.....................................................194,.196501624...............................................................194501629................................................................. 69501630................................................................. 69501631.................................................................. 69501632................................................................. 69501634................................................................. 69501635...............................................................169501636.............................................................. 209501637.............................................................. 209501638.............................................................. 209501639.............................................................. 209

501640.............................................................. 209501641.................................................................. 95501642................................................................. 95501643................................................................. 95501644..........................................................95,.97501651............................................................... 206501652.............................................................. 206501653.............................................................. 206501656................................................................. 95501657................................................................. 95501658................................................................. 95501669.............................................................. 209501670............................................................... 175501700.&.501700-G....................................... 116501701.&.501701-G........................................129501702.&.501702-G.......................................129501703.&.501703-G.......................................129501704.&.501704-G.......................................129501705.&.501705-G....................................... 116501706.&.501706-G....................................... 118501707.&.501707-G....................................... 118501708.&.501708-G....................................... 118501709.&.501709-G....................................... 117501710.&.501710-G........................................ 117501711.&.501711-G........................................ 117501712.&.501712-G........................................ 117501713.&.501713-G........................................ 117501714.&.501714-G........................................ 117501715.&.501715-G........................................ 117501716.&.501716-G........................................ 118501717.&.501717-G......................................... 118501718.&.501718-G........................................ 141501719................................................................ 141501720.&.501720-G....................................... 141501721.&.501721-G........................................ 141501722.&.501722-G....................................... 141501723.&.501723-G....................................... 141501724.&.501724-G....................................... 141501725.&.501725-G.......................................135501726.&.501726-G.......................................137501727-G............................................................ 111501728............................................................... 161501729..................................................... 142,.161501730.&.501730-G....................................... 117501731................................................................144501733.&.501733-G.......................................125501734.&.501734-G.......................................125501735...............................................................142501736...............................................................142501739.&.501739-G.......................................125501740.&.501740-G.......................................125501741-G............................................................ 110501742-G............................................................ 110501743.&.501743-G.......................................128501744.&.501744-G.......................................125501745.&.501745-G.......................................125501747.&.501747-G........................................125501748.&.501748-G.......................................125501749.&.501749-G.......................................126501750.&.501750-G.......................................126501751.&.501751-G........................................126501752.&.501752-G.......................................126501753.&.501753-G.......................................128501754.&.501754-G.......................................128501755.&.501755-G.......................................128501756.&.501756-G.......................................128501757.&.501757-G.......................................128501758.&.501758-G.......................................123501759.&.501759-G.......................................123501760...............................................................142501761................................................................142501762................................................................142501763................................................................142501764................................................................103501767................................................................124501768...............................................................124501770.&.501770-G....................................... 141501771................................................................154501772.&.501772-G.......................................149501773.&.501773-G.......................................149501775.&.501775-G.......................................109501776.&.501776-G.......................................109501777................................................................ 131501778................................................................ 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5377...................................................................... 845378...............................................................84,.965381.................................................................... 1785399...............................................................82,.965430................................................................... 2365435...................................................................... 825436...................................................................... 825440....................................................................1855444....................................................................1875447.........................................................................65450...................................................................... 685451...................................................................... 685464................................................197,.200,.2035468....................................................... 24,.27,.325469...................................................................... 245470...................................................................... 245475.................................................................... 2165479....................................................................2055482...................................................................... 245483...................................................................... 245489.........................................................................6555001FT.......................................................... 113555007FT.......................................................... 114555041FT.......................................................... 113555042FT.......................................................... 114555047FT.......................................................... 114555053FT..........................................................109555054FT..........................................................109555056FT..........................................................109555060FT.......................................................... 114555063FT.......................................................... 114555080FT.......................................................... 114555081FT.......................................................... 113555084FT.......................................................... 115555086FT.......................................................... 114555190FT........................................................... 114555222F............................................................. 116555223F............................................................. 116555227F.............................................................108555229F.............................................................108555230F.............................................................108555400NT.........................................................133555403NT.........................................................132555406NT.........................................................133555407NT.........................................................133555419NT..........................................................132555500S............................................................ 121555501S.............................................................122555526S............................................................ 121555541S............................................................. 121555561S............................................................. 121555580S............................................................ 121555582S............................................................ 121555583S............................................................ 121555584S............................................................ 121555620S............................................................122555640S............................................................120555641S............................................................. 121555646S............................................................124555656S............................................................129555801L.............................................................139555827L............................................................. 114555828L............................................................ 11456200.................................................................26256300.................................................................26258006.................................................................25358450.................................................................2535B120F-4............................................................1825B120F-6............................................................182600009..............................................................167600011................................................................. 84600012................................................................. 846820....................................................................167705............................................................................3711P................................................................27,.327128.....................................................................167712P..........................................................................4715P..........................................................................47325...................................................................... 827326...............................................................84,.967335....................................................................1677341....................................................................1687342....................................................................1687363....................................................................1647364....................................................................16474020................................................................... 4574030................................................................... 4574040................................................................... 4574050................................................................... 4575070................................................................... 307521...................................................................... 827600.............................................................. 68,.697600S............................................................ 68,.69765-HTP................................................................ 69



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77020................................................................. 181773............................................................................47B100F-4............................................................1827B120F-4............................................................182800100................................................................. 88800120.............................................................. 260800283.............................................................. 178800292................................................................. 2983016.....................................................................148-Channel.Digital.Stimulator............................12900A...................................................................... 78900AP................................................................... 78900APP................................................................. 78

AA300.Pulsemaster...............................................16A310.Accupulser..................................................15A320D....................................................................18A320R....................................................................18A320RC..................................................................18A360LA..................................................................19A362............................................................... 18,.21A365D....................................................................19A365R....................................................................19A365RC..................................................................19A382...................................................................... 20A385...................................................................... 20A385R................................................................... 20A385RC................................................................. 20A395.......................................................................21A395D....................................................................21A395R....................................................................21A395RC..................................................................21ABM....................................................................... 77ABSORBANCE...................................................257Accupulser™.Signal.Generator.........................15action.potentials..................................................10Adenosine.Sensor.............................................. 85Adhesives.................................................166,.167Adjustable.Pipetters.........................................234ADPT2.Footswitch............................................227Adson.Forceps................................................... 110Adson-Brown.Forceps...................................... 110aeration.manifold............................................... 49Ag/AgCl.Half-Cells............................................ 191Agricola.Retractor.............................................140AGTxxxx............................................................. 171AGWxxxx............................................................ 171Air-Therm............................................................ 218AL1000...............................................................227AL2000...............................................................227Aladdin.Syringe.Pumps...................................227Alconox...............................................................164Alligator.Forceps............................................... 118Alligator.scissors............................................... 131Allis.Tissue.Forceps.......................................... 117Alumina.Abrasive............................................... 32Amplifier.Selection.Guide...................................2amplitude.discriminator.....................................10Angled.Electrode.Holder................................200Animal.handling.gloves..................................153Animal.Temperature.Controller....................... 66APEH1.................................................................186APEH2.................................................................186Apollo.1000......................................................... 86Apollo.4000........................................................ 87Applicator.Spears..............................................154Applying.Forceps..............................................138Articulated.Arm........................................ 211,.213AS.Holder............................................................. 50ATC......................................................................... 66ATC1000............................................................... 66ATP.Sensor....................................................80,.85Audible.Baseline.Monitor................................. 77Automated.TEER.Measuring.System............. 62AUWxxxx............................................................ 171

BB.Holder................................................................ 50B203MC4.......................................................... 238B203XV............................................................. 238Ball.Bearing.Boom.Stand...................... 211,.213Ball.Joint,.Magnetic..........................................204Ball-joint.holder.attachment..........................201band.pass.filter................................................. 256Barb-to-Tubing.Coupler................................... 172Barraquer.Iris.Scissors..................................... 131Barraquer.needle.holder................................132base.plate...........................................................205Base,.Magnetic..................................................204BAT-10R/LOP........................................................ 64BAT-12R................................................................. 64

Bath.Chamber...................................................... 68Battery.Charger............................................18,.20Battery,.NiMH.........................................................7BD11E.................................................................. 170BD12E.................................................................. 170BDXR124............................................................ 170BDXR13.............................................................. 170BDXR13B............................................................ 170BDXR9................................................................. 170BEECAL.................................................................. 98Beetrode................................................................ 98BetterSkin™.Electrodes....................................190Beveler,.Micropipette......................................... 32Biemer.scissors.................................................. 131BIF22.................................................................. 259BIF41................................................................... 259BIF44.................................................................. 259BIF62.................................................................. 259BIF66.................................................................. 259Bifurcated.Fiber.Optic.Assemblies............... 259Bifurcated.Light.Guide..................................... 216Binocular.Microscope........................................ 29BINOCULARLOUPE..........................................155Bio-amplifier.......................................................6,.9Bio-amplifier.System.......................................... 44biocide.................................................................164Biopsy.Punch.....................................................154Biosensing............................................................ 79bipolar.stimulation............................................. 24Bishop.Harmon.forceps...................................109blade.remover...................................................143BLADES..........................................................27,.68Blades,.single.edge............................................ 68Blades,.Stainless.Steel....................................... 69blood.pressure..................................................... 42Blood.Pressure.Monitor..................................... 37BLOOD.PRESSURE.TRANSDUCER................. 37BLPR2.................................................................... 37Blumenthal.Rongeur........................................146Bone.Wax...........................................................154Boom.Stand.............................................. 211,.213Borosilicate..................................... 180,.182,.184BP1......................................................................... 37BPCABLE2............................................................. 37Brackets............................................................... 178Brain.Matrices...................................................... 27Bridge.Amplifier.................................................. 46Bridge8.................................................................. 45Brightfield........................................................... 215BT-1........................................................................ 65Buffer.Solutions.................................................100Bulldog.Clamps.................................................138

CC3005................................................................. 171Cables.and.Connectors................................... 174CAL900A.............................................................. 78CALBUF-1...........................................................100CALBUF-2...........................................................100Calcium.Calibration.Solutions.......................100Calcium.Electrode............................................. 101CALICELL-HTSF.................................................... 63CaliCell™............................................................... 60Caliper.................................................................149Caliper,.Digital...................................................169Camera................................................................221Cannula,.Micro..................................................184Cannulation.forceps......................................... 115capillary.glass....................................................180Carbon.Epoxy....................................................167Carbon.fiber.microelectrodes.......................... 94Carbon.Wire....................................................... 171Cardiac.Hemodynamics.................................... 38cartridge.electrodes........................................... 54Castroviejo.Needle.Holder.............................132Cautery................................................................157CBL100............................................................... 175CBL102............................................................... 175CC-3-UV............................................................. 258CCD.Video.Camera..........................................221CDOM................................................................. 248Cell.culture.cups................................................. 60Cell.culture.dishes.............................................. 76CELS....................................................................... 40Ceramic.Blades.................................................... 69Ceramic.Scissors...............................................129Ceramic-tipped.Forceps...................................108CF10-xxx............................................................... 94CF30-xxx.............................................................. 94CFN10-xxx............................................................ 94CFN30-xxx........................................................... 94Chamber,.Measurement................................... 88Chart.Recorders................................................. 170

CHM1.................................................................... 57CHM2.................................................................... 57CHM3.................................................................... 57CHM4.................................................................... 57CHM5.................................................................... 57CHM5M12............................................................ 57CHM6.................................................................... 57CHM7.................................................................... 57CHM8.................................................................... 57Chopstick.electrode........................................... 59Cidex.Plus...........................................................164Circulating.Bath................................................... 53Circulation.Reservoirs........................................ 57clamp...................................................................201Clamps................................................................. 176Cleaners..............................................................164Cleaning.Brushes..............................................164Clear.hose........................................................... 218Clip.Applier.........................................................137clip.removing.forceps......................................137CMF20GxxL.......................................................185CMF22GxxL.......................................................185CMF23GxxL.......................................................185CMF26GxxL.......................................................185CMF28GxxL.......................................................185CMF31GxxL.......................................................185CMF34GxxL.......................................................185CMF35GxxL.......................................................185CMF90UxxL.......................................................185C-Mount.................................. 211,.213,.214,.215coaxial.cables,.micro....................................... 171COLCAM..............................................................221COLLIMATOR.....................................................262Color.Video.Camera........................................221Combination.pH.electrode............................... 98Compact.Magnetic.Stand.............................. 206Compound.Microscope..................................... 29Concentric.Bipolar.Electrodes.......................... 24Concentric.Electrodes........................................ 25Conductive.Electrode.Gel...............................190Cooled.stage.insert.................................217,.219Copper.Alloy.Wire............................................ 171Corneoscleral.Punch........................................146Cover.Slips............................................................ 77CPVCxxxxx......................................................... 171Crile.Hemostatic.Forceps................................ 117Crile-Wood.Needle.Holder.............................134Crocodile.Forceps............................................. 118CU7T44G............................................................ 171Culture.dishes...................................................... 76Current.Clamp..............................................54,.58Current.Electrode................................................ 54Current.Generator................................................11Current.Sources....................................................19Cuticle.nipper....................................................147Cutters.................................................................145CUV1022-10......................................................257CUV2011-1........................................................257CUV2012-1........................................................257CUV2031-1........................................................257CUV2032-1........................................................257CUV2033-1........................................................257CUV2034-1........................................................257CUV2051-1........................................................257CUV2052-1........................................................257CUV2061-1........................................................257CUV2062-1.......................................................257CUV2071-1........................................................257CUV-ALL............................................................. 256Cuvette.Holders................................................ 256cuvettes...............................................................257CUV-MSP........................................................... 256CUV-UV.............................................................. 256CUV-UV-10........................................................ 256CUV-UV-FL......................................................... 256cyanoacrylate....................................................168

DD2H..................................................................... 260D2H-2................................................................. 260D2H-DB.............................................................. 260D2H-HB.............................................................. 260D2H-HBER......................................................... 260D2Lite..................................................................261DAM.Series.Bioamplifiers....................................6Dam50.....................................................................6Dam80.....................................................................7DAM80P..................................................................7data.acquisition..............................38,.70,.71,.74data.analysis........................................................ 74Data.Recorder.......................................................71Data-Trax.........................................38,.70,.72,.74DBA8000.............................................................. 46

DC.amplifier.system.......................................... 44DC3001L............................................................197DC3001R............................................................197DC3314L.............................................................197DC3314R.............................................................197DCAP....................................................................153DCAP-L................................................................153DCAP-M...............................................................153DeBakey.Forceps.............................................. 112Demagnetizer....................................................164Derf.Needle.Holder..........................................134DETECTOR.UPGRADEs................................... 250Deuterium.halogen.light.source.................. 260Deuterium.light.source...................................261Digital..Linear.Stimulus.Isolator......................14Digital.Bridge.Amplifier.................................... 46Digital.Caliper....................................................169Digital.Data.Recorder.........................................71Digital.Eyepiece.Camera................................220Digital.Micrometer...........................................169Digital.Micrometer.Head................................194Digital.Microscope.Camera............................220Digital.Stimulator................................................12Dilator.Forceps.................................................. 113DIP-NIRx.............................................................255Dipping.probe......................................... 255,.258DIP-UV-SRx.........................................................255DIP-UVx...............................................................255Displacement.Transducer................................. 40Disposable.Ag/AgCl.Snap.Electrodes..........190Disposable.Scalpel...........................................144Dissecting.Kit.....................................................156Dissecting.Knife................................................144Dissecting.Needles...........................................149Dissecting.Pad.Kit.............................................156Dissecting.Scissors...........................................123Dissection.Probes.............................................148Dissolved.oxygen.meter................................... 96DLS100..................................................................14DMF1000.Microforge........................................ 28DMF1000-H5....................................................... 29DMP.................................................................... 239drain.manifold..................................................... 49Dressing.Forceps............................................... 111DRIREF-2.............................................................100DRIREF-2SH.......................................................100DRIREF-450.......................................................100DRIREF-5.............................................................100DRIREF-5SH.......................................................100DRIREF-L.............................................................100Dri-Ref™...............................................................100Driven.guard.shield.........................................3,.5DRL..........................................................................19Dry.Sterilizer......................................................163DS8000......................................................... 12,.13DSPL...................................................................... 40Dual.Microiontophoresis.Current.Generator.11Dual.Microprobe.System.....................................4Dual.Range.Force.Transducer...........................41Dual.Tool-Holder.Micromanipulator............203Dual-View.Attachment.................................... 214Dummy.Load.Resistor.Kit..................................19Dummy.Membrane............................................ 58Dumont...............................................................103Duo.773..................................................................4DVC1000.............................................................. 58DVC2...................................................................... 58DVC3...................................................................... 58

EE210.....................................................................183E212.....................................................................183E215.....................................................................183E220....................................................................183Eagle.Pipetters...................................................234Ear.Bars................................................................. 23Ear.Punch............................................................145Ear.Tags...............................................................145Economy.Manual.Micromanipulator...........200EHB1....................................................................186EHBF....................................................................186EJA........................................................................ 216EK1..........................................................54,.57,.58EKC..........................................................54,.57,.58EKV..........................................................54,.57,.58EL203..................................................................190EL204..................................................................190EL204D...............................................................190EL204S................................................................190EL208..................................................................190EL208D...............................................................190EL208WD...........................................................190






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EL208WS............................................................190EL212...................................................................190EL212S.................................................................190Electro.705..............................................................3Electrolyte,.ISO2................................................. 96Electrolyte,.ISO-NO............................................ 82Electrometer............................................................3Electrosurgery.Systems...................................159Electrosurgical.Unit..........................................160Elgiloy.Microelectrodes..................................... 24ELS-370...............................................................261ELS-xxx................................................................261ENDOHM-12.........................................................61ENDOHM-24SNAP..............................................61ENDOHM-6...........................................................61Endohm™...............................................................61ENZOL.................................................................164Enzymatic.Detergent........................................164Enzymatic.Recycling.................................91,.263EP05..................................................................... 191EP08.................................................................... 191EP1....................................................................... 191EP12..................................................................... 191EP2....................................................................... 191EP4....................................................................... 191EP8....................................................................... 191Epithelial.Voltohmmeter................................... 59Epoxy.........................................................166,.167ES5.......................................................................234ES6.......................................................................234ES7.......................................................................234EVC3...................................................................... 54EVC4000.............................................................. 54EVOM™.................................................................. 59EVOMX.................................................................. 60EXT-6..................................................................... 64Extracellular.recording.........................................6Eye.Scissors........................................................127Eyepieces................................ 211,.213,.214,.215

FFD35-100............................................................. 76FD3510-100......................................................... 76FD3510B-100...................................................... 76FD35B-100........................................................... 76FD35COL-100..................................................... 76FD35PDL-100...................................................... 76FD5040-100........................................................ 76FD5040B-100..................................................... 76Fiber.Optic.Blood.Pressure.System................ 42fiber.optic.cables............................................. 259Fiber.Optic.Collimator.....................................262Fiber.Optic.Filter.Holder..................................262Fiber.Optic.Illuminator.................................... 216Fiber.optic.measurement.................................. 42Fiber.Optic.Spectrometers............................. 250Filter.Forceps...................................................... 115Filter.Holder,.Inline.......................................... 260Fire-Polished.......................................................180Five-barrel.glass................................................182Fixation.Forceps................................................ 111Flexible.Light.Guides....................................... 216Flexible.NO.Sensor............................................. 83FLEXREF..............................................................100Flow.cells............................................................257Fluorescence......................................................257Fluorescent.lamp.............................................. 211FluoroDish............................................................ 76FO-1000-SMA................................................... 259FO-1000-SMA1M............................................ 259FO-100-SMA..................................................... 259FO-100-SMA1M............................................... 259FO-200AS-SMA................................................ 259FO-200-SMA..................................................... 259FO-200-SMA1M............................................... 259FO-400AS-SMA................................................ 259FO-400-SMA..................................................... 259FO-400SMA/ST................................................ 259FO-400-SMA1M............................................... 259FO-50-SMA....................................................... 259FO-50-SMA1M................................................. 259FO-6000............................................................ 260FO-6000FILT..................................................... 260FO-600AS-SMA................................................ 259FO-600-SMA..................................................... 259FO-600-SMA1M............................................... 259FOBPS-13.............................................................. 43FOBPS-18.............................................................. 43FOBPS-28............................................................. 43FOIMPH...............................................................186FOIMPH-LF.........................................................186FOMS..................................................................... 43FOMS-13............................................................... 43

FOP1-SMA......................................................... 259FOP1-SMA/ST................................................... 259FOP1-ST............................................................. 259force.transducers.................................................41Forceps................................................................103FORT.transducers................................................41FORT100................................................................41FORT1000.............................................................41FORT10-100..........................................................41FORT25..................................................................41FORT250...............................................................41FORT5000.............................................................41FrameWorks....................................................... 176Free.Radical.Analyzer.................................86,.87Friedman.Rongeur............................................146Frog.Heart.Clips.................................................138

GGEL100...............................................................190Gelpi.Retractor................................................... 141Gillies.Dissecting.Forceps............................... 111Glass.bottom.dished.......................................... 76Glass.Capillaries................................................180Glass.Cuvettes...................................................257Glass.Handling.Forceps................................... 181Glass.Hook........................................................... 50glass.micropipettes..........................................184glass.rod..............................................................182Glass,.Holders.&..Electrodes.......................... 179Glucose.sensor.................................................... 83Glue.Gun...................................................167,.185Glutathione.Determination.....................91,.263GN-NET25..........................................................227GN-NET7.............................................................227GN-PC25.............................................................227GN-PC7................................................................227GN-TTL.................................................................227GO1-100.............................................................187GO2-100.............................................................187GO3-100.............................................................187GO4-100.............................................................187Gold.Wire............................................................ 171Gold-plated.pins,.sockets................................. 24Goldstein.Retractor..........................................140GR100-4.............................................................182GR100-6.............................................................182Graefe.Forceps...................................................109Grass.Transducer.Cable..................................... 46Griess.Reaction.Nitrite.Kit.......................92,.264GRNK...........................................................92,.264GRNK-II.......................................................92,.264GSH.Assay.Kit............................................91,.263GSH-KIT........................................................91,.263GSNO..................................................................... 89GSNO-100............................................................ 89GSNO-50.............................................................. 89GSNO-500........................................................... 89Guillotine............................................................153

HH602...................................................................... 29Haake.Circulating.Bath..................................... 53Half-Cells.............................................................186Heated.stage.insert.......................................... 219Heater.Controller.............................................. 218Heating.Circulator............................................... 53Heating.Coil......................................................... 34Heating.Filament.........................................29,.30Heating.Plate....................................................... 66high.pass.filter.................................................. 256high.throughput.screening............................... 62Hooks...................................................................148HS6......................................................................202HS6-3...................................................................197HS6-3M...............................................................197HT-1........................................................................ 65HT-2........................................................................ 65Hydrogen.Electrode......................................... 101Hydrogen.Peroxide.Determination......90,.262Hydrogen.Peroxide.Sensors......................80,.84Hydrogen.Sulfide.Sensor.................................. 80Hydrostatic.pressure....................................... 239

IIE010.................................................................... 101IE190................................................................... 101IE200................................................................... 101ILS.gas-tight.syringes............................ 230,.233implantable.probe.............................................. 95IMPLANTABLE.PROBES..................................... 65in.vitro.fertilization...........................................232IN1003................................................................ 171

Indium.Wire....................................................... 171Infrared.Blocking.Filter.................................... 216Infusion.Pump........................................ 228,.229Infusion/Withdrawal.Pump................. 227,.230injector................................................................232inline.filter.holder............................................ 260Inosine.Sensor..................................................... 85INST-APOLLO................................................86,.87Instrument.Portfolio.........................................162Instrument.Tip.Protectors...............................162IntegraSlice........................................................... 69Intracellular.Amplifier......................................3,.4intracellular.injection.......................................232Inverted.Microscope........................................ 214INV-IQ902.......................................................... 214INV-IQ902DIC................................................... 214INV-IQ903.......................................................... 214INV-IQ903DIC................................................... 214IO-KIT.................................................................. 240ion.selective.electrodes.................................. 101iontophoresis........................................................11Iridium.Microelectrodes.................................... 24Iris.Forceps..........................................................109Iris.Scissors.........................................................123IRM23Exxxx........................................................ 26ISDB.System........................................................ 44ISO2....................................................................... 96ISO2.Filling.Solution.......................................... 96ISO-80......................................................................8ISO-80P....................................................................8ISO-DAM..................................................................9Iso-DAM8A........................................................... 44ISO-DAMX...............................................................9ISO-H2S-2............................................................. 80ISO-HPO-100................................................80,.84ISO-HPO-100-H................................................... 80ISO-HPO-100-L.............................................80,.84ISO-HPO-2.....................................................80,.84Isolated.Bioamplifier.............................................8Isolated.Differential.Amplifier............................8ISO-NOP.........................................................80,.82ISO-NOP.Rejuvenator........................................ 89ISO-NOP007.................................................80,.83ISO-NOP30...................................................80,.83ISO-NOP30L........................................................ 84ISO-NOP70L.................................................80,.84ISO-NOPF.......................................................80,.83ISO-NOPFH........................................................... 80ISO-NOPNM..................................................80,.82ISO-OXY-100................................................80,.84ISO-OXY-100-H................................................... 80ISO-OXY-100-L.............................................80,.84ISO-OXY-2.....................................................80,.84Isostim....................................................................18ISO-TEMP-2.......................................................... 84IT-14....................................................................... 65IT-18....................................................................... 65IT-18EXLONG....................................................... 65IT-1E....................................................................... 65IT-21....................................................................... 65IT-23....................................................................... 65

JJansen.retractor................................................. 141JE671P................................................................... 99Joystick.control..............................196,.197,.202JUV......................................................................... 89

KKelly.Hemostatic.Forceps............................... 117KITE-L...................................................................200KITE-R..................................................................200KITE-TB-L.............................................................200KITE-TB-R.............................................................200KITLWCC.............................................................253KWIK-2................................................................ 101KWIKCAL-2......................................................... 101Kwik-Cast™.........................................................167Kwik-Gard™........................................................166KWIKGLUE..........................................................166KWIKGUN...........................................................166KWIKH-2............................................................. 101KWIKMIX............................................................166KWIKPOT-2........................................................ 101Kwik-Sil™.............................................................167Kwik-Tip™............................................................ 101KWIKTPP-2......................................................... 101KZ1101..................................... 37,.115,.152,.184

LL-32-32............................................................... 216L4......................................................................... 250

Lab.Coat..............................................................168Lab.Supply.Selection.Guide...........................165Lab-Trax..........................................................38,.70Lapping.Film,.Alumina.......................................31Lapping.Film,.Diamond......................................31leadscrew.positioner.......................................... 49LED.light.source................................................261LED-Lite...............................................................261Ligature.scissors................................................129Light.guides........................................................ 216Lighted.Fan.Base............................................... 210Linear.Stimulus.Isolator.....................................21Linear.Variable.Filters..................................... 256Liquid.Ion.Exchangers..................................... 101Liquid.Waveguide.Capillary.Cell...................252Lister.Bandage.scissors...................................129Live.cell.imaging............................................... 218Low.Noise.Preamplifier........................................9low.pass.filter................................................... 256Low-Pass.Filter..................................................... 77LPF30..................................................................... 77L-Shaped.Free.Radical.Sensor..........................51L-shaped.sensors................................................ 84Luer.Valve.Kit..................................................... 172Luer-to-Tubing.Coupler.................................... 172LUME..................................................................... 40LV140..................................................................227LWCC....................................................................252

MM1........................................................................204M10......................................................................205M10L....................................................................205M11......................................................................205M1L......................................................................204M2.................................................... 197,.201,.203M-3...................................................197,.200,.203M325...................................................................203M3301.................................................................200M3301EH.........193,.196,.197,.200,.202,.203M3301L..............................................................200M3301-M3-L......................................................200M3301-M3-R.....................................................200M3301R..............................................................200M4C..............................197,.200,.201,.202,.203M5.......................................... 197,.201,.203,.204M6.................................................... 197,.201,.203M7....................................................................... 206M8........................................................................204M9........................................................................205Macro.sensors..................................................... 80Magnetic.Ball.Joint...........................................204Magnetic.Base.................................................. 206Magnetic.Heating.Stirrer.................................169Magnetic.Holding.Devices.................. 204,.206Magnifying.Glasses..........................................155Manometer........................................................ 239Manual.Micromanipulator..............................200Manual.Microsyringe.Pump.......................... 239Mathieu.Fine.Needle.Holder.........................134Mayo.Scissors....................................................126MB2......................................................................204MBMPH5-7..............................................183,.186MBS.........................................................................31McIndoe.Dressing.Forceps............................. 111MCL3-HS6..........................................................197MCL3-SM325....................................................196McPherson-Vannas.Scissors........................... 119MD4L...................................................................203MD4-M3-L..........................................................203MD4-M3-R..........................................................203MD4R...................................................................203megohm.meter................................................... 27MEH145..............................................................186MEH1F45...........................................................186MEH1R................................................................186MEH1RF..............................................................188MEH1S................................................................188MEH1SF..............................................................188MEH2R................................................................188MEH2RF..............................................................188MEH2RFW..........................................................188MEH2RW............................................................188MEH2S................................................................188MEH2SF..............................................................188MEH2SFW..........................................................188MEH2SW............................................................188MEH345.............................................................188MEH3F45...........................................................188MEH3FW45.......................................................188MEH3R................................................................188MEH3RF..............................................................188MEH3RFW..........................................................188




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MEH3RW............................................................188MEH3S................................................................188MEH3SB..............................................................188MEH3SBW..........................................................189MEH3SF..............................................................189MEH3SFW..........................................................189MEH3SW............................................................189MEH3W45.........................................................189MEH6RF..............................................................189MEH6RFW..........................................................189MEH6SF..............................................................189MEH6SFW..........................................................189MEH7...................................................................189MEH7W...............................................................189MEH8...................................................................189MEH900R...........................................................189MEH900S...........................................................189MES........................................................................ 32Metal.Microelectrodes....................................... 24Metzenbaum.Scissors......................................125Meyhoefer.Curette...........................................149MF200.Microforge............................................. 30MF200-H2............................................................ 30MF200-H3............................................................ 30MF200-H4.....................................................29,.30MF28G-5............................................................185MF28G67-5.......................................................185MF34G-5.............................................................185Micro.Burr...........................................................160Micro.Cannula........................ 37,.115,.152,.184Micro.Coaxial.Cables....................................... 171Micro.Hemostatic.Forceps.............................. 116Micro.pH.Electrodes........................................... 98Micro.Sensors.for.NO........................................ 80Micro.Tissue.Holder............................................51Micro.Tweezer................................................... 111Micro4.Controller................................... 233,.238MicroBeveler.........................................................31MicroC................................................................... 93MICROCP.............................................................. 93Microdialysis.Pumps....................................... 228MICRODRILL......................................................160Microelectrode.Beveler..................................... 32Microelectrodes................................................... 24MicroFil................................................................185MicroFlow............................................................. 95Microforge.................................................... 28,.30MicroImplant........................................................ 95Microintraocular.forceps................................. 115Microintraocular.scissors................................130Microiontophoresis..............................................11Micromanipulator...............193,.196,.197,.200Micrometer.Eyepiece....................................... 215Micrometer.Head..............................................194Micrometer.Slide.Micromanipulator............203Micrometer.Transducer.Positioner................. 49Micrometer,.Digital..........................................169Micropipette.Beveler.......................................... 32Micropipette.Blanks.........................................183Micropipette.Calibration................................... 28Micropipette.Holders.......................................186Micropipette.puller......................................33,.34Micropipette.Storage.......................................183Micropositioner.................................................198Micropressure.System....................................... 78Microprobe.Thermometers.............................. 64Micro-Reference.Electrode.............................100microscope.stage..............................................201Microscope.Stage.Adapter.............................201Microscopes.and.Cameras............................ 208Microsurgical.Knife..........................................144microsyringe......................................................233MicroTip................................................................ 95Microtome............................................................ 69Microvolume.Syringes.....................................233Millivolt.&.megohm.meter............................... 27Mini.Sensors........................................................ 80Miniature.Micropositioners............................199Miniature.Peristaltic.Pump.............................226MiniFlow................................................................ 95MiniFoil.................................................................. 95MINISTAR...........................................................226MiniTip.................................................................. 95MityFlex.Peristaltic.Pump...............................226MITY-KIT..............................................................226Mixter.Hemostatic.Forceps............................. 118MM1....................................................................199MM3....................................................................199MM3-MCPRO.....................................................198MM3-MSPRO.....................................................199MMJL...................................................................202MMJR...................................................................202MMP.................................................................... 239MMP-KIT............................................................ 239

MOD-BMH-BF.................................................... 215MOD-TMH-BF-V................................................. 215modular.DC.amplifier........................................ 44Modular.Microscopes...................................... 215Monochrome.Video.Camera.........................221Mosquito.Hemostatic.Forceps....................... 116Motorized.Micromanipulator...............196,.197Motorized.Micropositioner.............................199Mouse.Adaptor.................................................... 23Mouse.Kit............................................................ 151Mouse.Vessel.Cannulation.Kit......................152Mouse/Neonatal.Rat.Adaptor......................... 23Mouse/Rat.Eye.Speculum..............................139MPH1...................................................................189MPH3...................................................................189MPH4...................................................................189MPH6P................................................................189MPH6R................................................................189MPH6S................................................................189MPH8...................................................................195MPM10................................................................195MPM20...............................................................195MPS-2.................................................................... 67MS314.................................................................197MSI-150.............................................................. 216MT-23/3................................................................ 65MT-23/5................................................................ 65MT-23/8................................................................ 65MT-26/2................................................................ 65MT-26/4................................................................. 65MT-26/6................................................................ 65MT-29/1................................................................. 65MT-29/1B.............................................................. 65MT-29/2................................................................ 65MT-29/3................................................................ 65MT-29/5................................................................ 65MT-4....................................................................... 65MT-D....................................................................... 65Multi-96.................................................................61Multi-Barrel.Glass..............................................182Multi-barrel.micropipette.coupling...............185Multichannel.Perfusion.System...................... 67Multi-Channel.Stimulator...........................16,.17Multi-Channel.Tissue.Bath............................... 48Multipipette.Puller.............................................. 33multi-port.measurement.chamber................. 88MULTISCOPE...................................................... 215multistage.puller................................................. 34Myobath.II............................................................ 48

NNail.nippers........................................................147NANOFIL............................................................ 240Nanoliter.2000................................................ 238Nanoliter.Injector............................................. 238NanoSensor.......................................................... 82Needle.Holder...................................................132NEEDLE.MICROPROBES................................... 65Needles...............................................................136Neonatal.Rat.Adaptor........................................ 23Neurotransmitter.Detection............................. 93NF33-36BL.........................................................241NF33-36BV........................................................241NF33BL-2............................................................241NF33BV-2...........................................................241NF33FBL-2.........................................................241NF33FBV-2.........................................................241NF34BL-2............................................................241NF34BV-2...........................................................241NF35BL-2............................................................241NF35BV-2...........................................................241NF36BL-2...........................................................241NF36BV-2...........................................................241NFGSK-5.............................................................241NFINHLD.............................................................241NFQ34-5.............................................................241Nipper..................................................................147NITRALYZER.II...........................................92,.264Nitrate.to.Nitrite.Reduction....................92,.264Nitric.Oxide.Sensor.Guide................................ 82Nitric.Oxide.Sensors.......................................... 80NMPH1.................................................................. 98NMPH2.................................................................. 98NMPH2B............................................................... 98NMPH2-LF............................................................ 98NMPH3.................................................................. 98NMPH3L............................................................... 98NMPH5.................................................................. 98NOCHM-4............................................................. 88NOCHM-P............................................................. 88Non-magnetic.bases........................................ 176Non-Rotating.Micrometer...............................194NOVA-186.......................................................... 216NOVAFLEX.......................................................... 216

Noyes.Scissors...................................................122NSA-3.................................................................... 89NVSL...................................................................... 68NVSLM1................................................................ 68

OO.Ring.................................................................... 32Octyl.cyanoacrylate.........................................168OFLV-200-850................................................. 250OFLV-350-1000............................................... 250Olsen-Hegar.Needle.holder...........................135Omega-Tip-Z........................................................ 27OMEGA-Z.............................................................. 27Oocyte.injection.............................................. 238Open.Circuit.Potential....................................... 54Operating.Scissors............................................128Ophthalmic.Knife..............................................144OR320................................................................... 99OR720................................................................... 99Organ.bath........................................................... 48Original.Pipetter.Stand....................................234OXELP.................................................................... 96oxygen.dipping.probe....................................... 95oxygen.electrode................................................ 96Oxygen.Sensors...........................................80,.84OxyMicro.............................................................. 95OxyMini................................................................. 95

PP-5-10..................................................................183P-5-50..................................................................183P-7-10..................................................................183Patch.Clamp....................................................... 181PB150F-4............................................................182PB150F-6............................................................182Peltier.microscope.stage................................. 217perfusion.chambers........................................... 56Perfusion.System................................................ 67Peristaltic.pump..................................... 224,.226Peri-Star.Pro.......................................................224PeroxiLuminol............................................90,.262Pette-Clamp........................................................234PG10150-4......................................................... 181PG10165-4......................................................... 181PG52150-4......................................................... 181PG52151-4......................................................... 181PG52165-4......................................................... 181pH.electrode................................................ 98,.99pH.Meter........................................................97,.99pH.Meter,.Fiber.Optic........................................ 97pH.mini.sensors.................................................. 97pH/ISE.Meter....................................................... 99Phaco.Sapphire.Blade......................................142PH-OPTICA-MICRO............................................. 97PH-OPTICA-MINI................................................. 97pHOptica™............................................................ 97Photo.Tube......................................................... 214Photocell............................................................... 40pH-Silver................................................................ 98Physiology............................................................ 35Physiology.Kit.I.................................................150Physiology.Kit.II................................................150PI-305A...............................................................183PicoNozzle.Kit................................................... 236PiezoPatch..........................................................193PiezoPatch™.Micromanipulator.....................193Piezo-Translator.................................................195Piggyback...........................................................182Pipette.Tips.........................................................235Pipetter.Rack.Stand..........................................234Pipetters..............................................................234Planachromatic.Objective............................... 211Platinum./.Iridium.Teflon.Wire...................... 171Platinum./.Iridium.Wire................................... 171Platinum.Wire.................................................... 171Platinum-iridium.Microelectrodes.................. 24PM.Series........................................................... 239PM01................................................................... 239PM015................................................................ 239PM100................................................................ 239PM5............................................................ 195,.197PM6......................................................................195PM7......................................................................195PMP-107............................................................... 33PNEU.transducers............................................... 40PNEU01................................................................. 40PNEU05................................................................ 40PNEU15................................................................. 40Pneumatic.PicoPump...................................... 236Pointer.Eyepiece............................................... 214Polishing.Patch.Pipettes.................................... 29PolyFil..................................................................185Post.Stand........................................................... 213

Potassium.Electrode......................................... 101Potentiostat.......................................................... 93POWERCORDS.................................................. 175PPM5000...........................................................193Preamplifier...................................................54,.58Precision.Current.Sources..................................19Precision.SurgioScope.................................... 209Pre-polarizer......................................................... 89Pressure.Ejection.Kit........................................183Pressure.Manometer....................................... 239Pressure.Pod........................................................ 78Pressure.Sensor................................................... 40Probes..................................................................148Programmable.Syringe.Pump.......................227PSMB5................................................................ 209PSMT5................................................................ 209PTM123Bxxxx..................................................... 26PTM23xxxx.......................................................... 25PTPxxx................................................................. 171PTTxxxx.............................................................. 171PTxxxx................................................................. 171PUC.......................................................................168PUC-Y...................................................................163PUL-2..................................................................... 34Puller,.Programmable........................................ 33pulse.generator............................................ 15,.16Pulse.Train.............................................................16Pulsed.Ultrasonic.Cleaner....................163,.168Pulsemaster™Multi-Channel.Stimulator.........16Pump.Selection.Guide.....................................223Pumps.&.Fluid.Handling.................................222Push-Pull.Pump.................................................231PV820.................................................................237PV830................................................................ 236PZMIII.................................................................. 212PZMIII-AAC......................................................... 212PZMIII-BS............................................................ 212PZMIV.................................................................. 210PZMIV-BS............................................................ 210PZMTIII................................................................ 212PZMTIII-AAC....................................................... 212PZMTIII-AAC-CCTV........................................... 212PZMTIII-BS.......................................................... 212PZMTIII-BS-CCTV.............................................. 212PZMTIII-CCTV.................................................... 212PZMTIV................................................................ 210PZMTIV-BCS....................................................... 210PZMTIV-BS.......................................................... 210PZMTIV-CCTV.................................................... 210

QQuartz.Cuvettes.................................................257

RRackmounting.Hardware................................ 178Rat.Kit.................................................................. 151Razor.Blade.Holder..........................................144RBMA-xxxx.......................................................... 27RBMS-xxxx........................................................... 27RC1....................................................................... 191RC1T.................................................................... 191RC2....................................................................... 191RC2F..................................................................... 191RC3....................................................................... 191RC4....................................................................... 191RC5....................................................................... 191RC6....................................................................... 191RECTAL.PROBES................................................. 65Reference.Electrodes.......................................100Reflectance.Probes.......................................... 258Reflex.Clip..........................................................137Reflex.Clip.Applier............................................137REF-UV............................................................... 258REMS..................................................................... 63REMS.AutoSampler............................................ 62REMS-24............................................................... 63REMS-96............................................................... 63REMS-KIT.............................................................. 63REMS-KIT-M......................................................... 63Replacement.Electrode.Holder.....................200Replacement.Needles......................................233Replacement.Tubing........................................225Resistance.measurement...................................61RET-2...................................................................... 65RET-3...................................................................... 65Retractors............................................................139Ribbon.Handle.Iris.Scissors............................124Ring.Light............................................................ 216Ring.Light.Adapter............................................ 216Ring.Light.Guide............................................... 216Ringstand.Mounting.Kit.......................................7Rochester-Carmalt.Hemostatic.Forceps...... 118Rochester-Oschner.Hemostatic.Forceps..... 117






PR

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UC

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X

271

Rochester-Pean.Hemostatic.Forceps............ 118Rodent.Brain.Matrices....................................... 27Rods,.stainless.steel......................................... 176Roger.Wirecutting.Scissors.............................169Rongeur...............................................................145RPE-KIT............................................................... 240RTV.Coating.......................................................167RTV.Prime.Coat.................................................167RTV.Sealant........................................................167Russian.Forceps................................................. 112Ryder.Needle.Holder.......................................134

Ssample.cell.............................................. 248,.254Sapphire.Knife...................................................142SBT-5...................................................................... 64Scalpel.Blades...................................................143Scalpel.Handle..................................................143Scissors................................................................ 119Scotch-Weld.......................................................166Screwdriver.Set.................................................169SDR2....................................................................100Self-retaining.Retractor...................................140Senn.Retractor................................................... 141Sensor.Guide........................................................81Septum.Theta....................................................182Seven-barrel.glass............................................182SGE.syringes......................................................233SI-40-8................................................................ 216SI-72-8................................................................ 216Signal.Generator..................................................15SILFLEX-2...........................................................241Silicone.RTV.......................................................167Silicone.Tubing....................................... 225,.226Silver.Clips..........................................................137Silver.Epoxy.......................................................167Silver.Wire.......................................................... 171Single.Barrel.......................................................180Skin.Stapler........................................................137SLIT-xxx.............................................................. 250SM325................................................................196SM325-M............................................................196SNAP...................................................................... 89SNAP100.............................................................. 89S-nitrosoglutathione.......................................... 89S-Nitroso-N-acetyl-D,L-penicillamine............. 89SP100i................................................................ 228SP101i................................................................ 228SP120p................................................................231SP200i.................................................................229SP210c.................................................................231SP210iw............................................................. 230SP220i.................................................................229SP230iw............................................................ 230SP250i.................................................................229SP260p...............................................................231Specimen.Holder................................................ 68SPECTRA4-HR................................................... 250SPECTRA4-HR-CG............................................ 250SpectraSuite.......................................................251SPECTRAUSB4.................................................. 250SPECTRAUSB4-CUV.........................................251SPECTRAUSB4-FL.............................................251SPECTRAUSB4-FLG......................................... 250SPECTRAUSB4-LWCC......................................251SPECTRAUSB4-M2.......................................... 250SPECTRAUSB4-NIR......................................... 250SPECTRAUSB4-SPT..........................................251SpectraView.......................................................245spectrometer........................................... 244,.250Spectrometer,.Miniature................................ 250SpectroPipetter..................................................254SPECTROSCOPY................................................242Speculum............................................................140Spring.Scissors..................................................122SPT.......................................................................254SPT-2....................................................................254SPT-2-UCK..........................................................254SPT-UCK..............................................................254SS316xx.............................................................. 171SSM123Bxxxx..................................................... 26SSM33xxxx.......................................................... 26SSTxxxxx............................................................ 171stability.button,.SP200.Series...................... 230stage.inserts....................................................... 219Stage.Micrometer...........................211,.214,.215Stainless.Steel.Rods......................................... 176Stainless.Steel.Wire.......................................... 171Staple.Remover.................................................137Steel.Base.Plate.................................................205Stereo.Zoom.Microscope.......................210,.212Stereotaxic............................................................ 22Sterilizer.tray............................................ 106,.161

Stevens.Needle.Holder...................................132Stevenson.Retractor.........................................140stimulator....................................................... 15,.16Stimulator,.Digital...............................................12Stimulator/Isolator...............................................18Stimulus.Isolator............................14,.19,.20,.21Stirrer...................................................................169STM3....................................................................197Stopcock............................................................. 173Student.Vannas.Scissors................................. 131Student.tweezers..............................................107STX100................................................................. 60STX100C............................................................... 60STX100C96......................................................... 60STX100F............................................................... 60STX100F96.......................................................... 60STX100M.............................................................. 60STX2...................................................................... 59STX3...................................................................... 59Sub-microliter.injection.................................. 240Super.Adhesive.................................................168SUPER-Dri-Ref....................................................100SuperLuminol...................................................... 90Superoxide.Determination............................... 90Surgery.Table.....................................................156Surgical.&Microdissection.Tools...................102Surgical.Instrument.Lubricant.......................164Surgical.Needles...............................................136SurgioScope...................................................... 209Suturing.forceps................................................ 114SVC108................................................................. 49Sylgard................................................................166Syringe.Pumps....................................... 227,.228Syringes.with.Luers............................... 230,.233Syringes.with.Needles.....................................233

TTAXIC-600............................................................ 23TAXIC-603............................................................ 23TAXIC-650............................................................ 23TAXIC-653............................................................ 23TB-1......................................................................200TBM4M.................................................................. 36TBS............................................................. 197,.201TCJ3050............................................................. 171TCK3050............................................................ 171TEER.measurement......................59,.60,.61,.62Teflon-Coated.Cupped.Forceps..................... 112Temperature.Controller..................................... 66Temperature.Meter............................................. 99temperature.probe........................................... 218Temperature.Probes........................................... 64Temperature.Sensor........................................... 84Temperature.Transducer................................... 40Tenotomy.Scissors............................................125TGWxxxx............................................................ 171Thermal.Cautery...............................................158Thermal.Microscope.Stage............................ 217Thermocouple.Wire......................................... 171Thin.Wall.............................................................180Three-barrel.glass.............................................182TidasDAQ............................................................245TIDASI..................................................................244TIDAS-I-LWCC....................................................247TIDAS-I-LWCC5..................................................247TIDAS-I-SPT........................................................247TIDAS-I-TIP..........................................................247TIDAS-I-TIPUV....................................................247TIDAS-QUAD......................................................247Tilt.Base..................................................... 197,.201Tilt.Base.Assembly..............................................31TIP01TW1F.........................................................184TIP01TW1F-L.....................................................184TIP02TW1F........................................................184TIP02TW1F-L.....................................................184TIP03TW1F........................................................184TIP03TW1F-L.....................................................184TIP04TW1F........................................................184TIP04TW1F-L.....................................................184TIP05TW1F........................................................184TIP05TW1F-L.....................................................184TIP10TW1...........................................................184TIP10TW1-L.......................................................184TIP10TW1LS01.................................................184TIP10TW1LS02.................................................184TIP10XV119.............................................184,.238TIP1TW1.............................................................184TIP1TW1-L.........................................................184TIP2TW1.............................................................184TIP2TW1-L.........................................................184TIP30TW1..........................................................184TIP30TW1-L.......................................................184TIP30TW1LS01.................................................184

TIP30TW1LS02................................................184TIP5TW1.............................................................184TIP5TW1-L.........................................................184TIP5TW1LS01....................................................184TIP5TW1LS02...................................................184TIPCA................................................................... 101TIPH...................................................................... 101TIPK...................................................................... 101TIPMIX01-05.....................................................184TIPMIX01-05-L..................................................184TIPMIX05-10.....................................................184TIPMIX05-10-L..................................................184TIPTPP.................................................................. 101Tissue.Bath........................................................... 49Tissue.Bath.Cooler............................................. 69Tissue.Forceps................................................... 110Tissue.Holders......................................................51Titanium.Forceps..............................................108Titanium.wire..................................................... 171TIxxxx.................................................................. 171TM123Axxxx....................................................... 26TM31xxxx............................................................ 25TM33xxxx.....................................................25,.26TM53xxxx.....................................................25,.26Towel.Clamp...................................................... 116TPC-4/PTU.......................................................... 217TPC-4ER/PTU..................................................... 217TPC-4LPD............................................................ 217TPC-4SPD........................................................... 217TPP.Electrode..................................................... 101TR600TIP........................................................... 258TR600-xx........................................................... 258Trace.Analysis....................................................247Trans.Epithelial.Electric.Resistance................ 59Transbridge.......................................................... 36Transducer.Amplifier...................................36,.45Transducers.......................................................... 40Transmission.probes............................. 255,.258Transparent.Heated.Stage.Insert.................. 219Trinocular.Head................................................ 214Troutman.needle.holder.................................133TR-TIP-xx............................................................ 258TR-UV.................................................................. 258TS-4...................................................................... 217TS-4ER................................................................. 217TS-4SPDER......................................................... 217TST150-6............................................................182TST33xxxx....................................................25,.26TST53xxxx....................................................25,.26TTL.Control.Module.............................. 225,.226Tubing.Cartridge...............................................225Tungsten.Halogen.lamp.................................. 211tungsten.light.source...................................... 260Tungsten.Microelectrodes................................ 24Tungsten.Wire................................................... 171TurtleSkin.Gloves..............................................153TW100-3.............................................................180TW100-4.............................................................180TW100-6.............................................................180TW100F-3...........................................................180TW100F-4...........................................................180TW100F-6...........................................................180TW120-3.............................................................180TW120-4.............................................................180TW120-6.............................................................180TW120F-3...........................................................180TW120F-4...........................................................180TW120F-6...........................................................180TW150-3............................................................180TW150-4............................................................180TW150F-3..........................................................180TW150F-4..........................................................180TW150F-6..........................................................180Tweezers.............................................................103Two-barrel.glass................................................182Two-stage.puller................................................. 34Tying.Forceps..................................................... 114Tyrell.Hook.........................................................149

UUBATH.................................................................163UCK........................................................... 254,.255ultra-fine.wire.................................................... 171UltraMicroPump.II............................................232UltraPath............................................................ 248Ultrasonic.Cleaner..................................163,.168UMP2...................................................................232UMS.....................................................................194Uni-Core.Punches.............................................154Universal.Focus.Mount................................... 213Universal.Manipulator.Stand.........................194Universal.Pipette.Tips......................................235UPUV...................................................................249

UPVIS...................................................................249USB.CAM-3M.....................................................220USBCON............................................................. 250USBUV................................................................ 250USBVIS............................................................... 250USS1L.................................................................... 57USS1S.................................................................... 57USS2L.................................................................... 57USS2S.................................................................... 57USS3L.................................................................... 57USS3S.................................................................... 57USS4L.................................................................... 57USS4S.................................................................... 57USS5L.................................................................... 57USS5M12L............................................................ 57USS5M12S........................................................... 57USS5S.................................................................... 57USS6L.................................................................... 57USS6S.................................................................... 57USS7L.................................................................... 57USS7S.................................................................... 57USS8L.................................................................... 57USS8S.................................................................... 57Ussing.System..................................................... 56Ussing.Tubing.Kit................................................ 57µTip......................................................................184Utility.eye.scissors............................................124Utility.Scissor.....................................................129UV.Safety.Goggles.......................................... 260UV/VIS.................................................................247UV4..................................................................... 250

VV.Holder................................................................ 50Vacuum.Pump...................................................227Vannas.Scissors................................................. 119variable.filter..................................................... 256Vascular.Measuring.Ruler...............................149VCAMBW............................................................221Vessel.Cannulation.Forceps........................... 115Vessel.Cannulation.Kit....................................152Vessel.Clips.........................................................138vessel.holders.......................................................51Vetbond...............................................................168Vibration-Free.Tables.......................................207Vibroslice.............................................................. 68Video.Cameras..................................................221Voltage.Clamp..............................................54,.58Voltage.Electrode............................................... 54Voltage/Current.clamp...............................54,.58VS.Holder.............................................................. 50VSLM1C................................................................ 68VSLM1H................................................................ 68

WWafer.Handling.Forceps.................................. 116Wall.Mount.Plate..................................... 211,.213Water.Bath............................................................ 57Waveguide.Cleaning.Kit...................... 249,.253Wecker.Spatula.................................................149Weitlaner.Retractor.......................................... 141Westcott.Stitch.Scissor..................................... 121Window.Discriminator.......................................10Wire...................................................................... 171Wire.Cutter...............................................147,.169Wire.Retractors..................................................139Wirecutting.Scissors.........................................130WPI-118..................................................................71WSA1001.............................................................. 85WSA1002............................................................. 85WSA1003............................................................. 85WSA1004............................................................. 85WSA2001............................................................. 85WSA2003............................................................. 85

XxxxxD.................................................................... 57

ZZBEECAL............................................................... 98Zoom.Microscope....................................210,.212




272

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Ordering InformationOrders.from.North Americashould.be.directed.to.WPI’s.main.office:.World.Precision.Instruments,.Inc.,.175.Sarasota.Center.Boulevard,.Sarasota,.Florida.34240-9258..Telephone:.941-371-1003.(collect.calls.not.accepted)..Facsimile.copies.of.purchase.orders,.inquiries.or.other.correspondence.may.be.transmitted.to.WPI’s.Fax.number:.941-377-5428..When.sending.written.purchase.orders.to.confirm.a.telephone.order,.please.clearly.mark.“CONFIRMING”.on.the.order.to.avoid.it.being.shipped.a.second.time..Our.Sales.Department’s.e–mail.address.is:[email protected]

North.American.customers.may.also.order.many.products.on-line.through.WPI’s.Web.site.—.www.wpiinc.com..All.customers.may.use.the.on-line.Quote.Request.Form.and.on-line.Purchase.Order.Form.

Orders.from.Belgium,.Denmark,.Eire,.Finland,.France,.Luxembourg,.Norway,.Portugal,.Spain,.Sweden,.Switzerland.(FR).and.the.United.Kingdom.should.be.directed.to.WPI’s.UK.office:.Astonbury.Farm.Business.Centre,.Aston,.Stevenage,.Herts.SG2.7EG.England..Tel:.+44.(0)1438-880025..Fax:.+44.(0)1438-880026..E-mail:[email protected]

Orders.from.Austria,.Germany,.Greece,.Italy,.Netherlands,.Poland,.Russia,.Switzerland.(DE),.Turkey,.and.Eastern.Europe.should.be.directed.to.WPI’s.German.office:.Liegnitzer.Str..15,.D-10999.Berlin,.Germany..Tel:.+49.(0)30-6188845..Fax:.+49.(0)30-6188670..E-mail:[email protected]

Orders.from.the.countries.listed.below.should.be.directed.to.the.appropriate.WPI.distributors.

All.other.international.orders.should.be.directed.to.WPI’s.main.office.and.factory.in.Sarasota,.Florida.

To.expedite.shipment.of.orders.outside.North.America.(other.than.to.areas.served.by.the.international.offices.above),.payment.must.be.made.in.advance.via.wire.transfer,.check.payable.in.U.S..dollars,.or.charged.to.a.credit.card..Letter.of.Credit.will.also.be.accepted.for.any.order.over.$10,000.($600.processing.fee.will.apply)..Request.instructions.for.issuing.an.acceptable.Letter.of.Credit.before.opening..Proforma.Invoices.will.gladly.be.furnished.upon.request..Please.specify.proper.electrical.current.for.your.order.





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Terms & ConditionsWPI values its customers and will make every effort to maintain the highest professional and ethical standards. However, WPI regards as invalid any special terms and conditions set forth by any other party, including purchasing, quotations, warranties or other statements. WPI’s response to a quotation, purchase order or inquiry shall not be construed as acceptance of any conditions stated therein.

Credit TermsNet 30 days from date of invoice to customers with satisfactory credit references.

Standard Equipment & AccessoriesTo insure against misunderstandings or delays, always include model number, name, and significant specifications of systems and accessories when ordering. Also specify any options desired. WPI retains the right to change specifications or discontinue products without prior notice and without responsibility for incorporating such changes into equipment previously sold. Please state the desired operating line voltage and frequency.

WarrantyWPI strives to maintain the highest quality standards in all products. We warranty these products against defects in workmanship or materials, and all WPI products retain a warranty that is stated in the product’s manual. (Typically, one year for parts and labor except for consumable items such as electrodes, glass, etc., for which warranty varies from 30 days to six months.) However, WPI hereby specifies that no use or application of any product may be suitable or successful for a specific application and therefore waives all liability in that regard. It is the sole responsibility of the buyer/user to apply the product in a manner consistent with its intended use.

WPI products are not approved for human use unless specifically stated in writing with accompanying FDA (or applicable) documents. WPI assumes no liability or legal, moral, ethical, or fiduciary responsibility for a use of any product in the WPI catalog in treating or studying humans.

PricesWPI maintains competitive world wide pricing. Prices listed for standard items as described are net and subject to change without notice. Prices (in U.S. dollars) do not include shipping charges. Formal quotations are valid for a period of 30 days unless otherwise specified. Purchases may be charged to your VISA, MasterCard, or American Express account.

TaxesWe reserve the right to add any taxes required by local law or ordinance. Institutions operating under taxfree conditions must state applicable license or contract number and be prepared to furnish necessary exemption certificate.

Packaging and ShippingEquipment is shipped in adequate commercial packing. All packages should be checked thoroughly before being discarded. Shipping and handling expenses, including adequate insurance coverage, are paid by the customer.

Claims and ReturnsInspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing. Concealed loss or damage should be reported at once to the carrier and an inspection requested. All claims for shortage or damage must be made within ten (10) days after receipt of shipment. Claims for lost shipments must be made within thirty (30) days of receipt of invoice or other notification of shipment. Please save damaged or pilfered cartons until claim is settled. In some instances, photographic documentation may be required. Some items are timesensitive; WPI assumes no extended warranty or any liability for use beyond the date specified on the container

Do not return any goods to us without obtaining prior approval and instructions from our Returns Department. Goods returned unauthorized or by collect freight may be refused. Goods accepted for restocking will be exchanged or credited to your WPI account and are subject to a restock fee of up to 25%. Goods returned which were ordered by customers in error are subject to a 25% restock fee. Equipment which was built as a special order cannot be returned.

RepairsContact our Returns Department for assistance in the repair of apparatus. Do not return goods until instructions have been received. Returned items must be securely packed to prevent further damage in transit. The Customer is responsible for paying shipping expenses, including adequate insurance on all items returned for repairs. Identification of the item(s) by model number, name, serial number and proof of purchase (packing slip number or invoice number) as well as complete description of the difficulties experienced should be written on the RMA request form and a copy of the form must be included with the item.

Keep fingerprints off your capillaries with these Glass

Handling ForcepsSee page 181

Bases, Rods & ClampsSee page 176

PiezoPatch™ Micromanipulator

See page 193

Implantable

Glucose Sensor

See page 83

Animal Temperature Controller

See page 66

Physiology—Cardiovascular

—page 35

Neuroscience—page 2

Digital Peristaltic PumpSee page 224

Multi-channel Tissue Bath

See page 48

Copyright © 2007, World Precision Instruments, Inc. All rights reserved. Printed in U.S.A.

Micro-manipulators

—page 192

Microdissection,Microsurgery

—page 102

Microscopes,Cameras

—page 208

Spectroscopy, Fluorometry

—page 242

Index—page 265

Pumps, Fluid Handling

—page 222

Laboratory Supplies

—page 165

Glass, Holders,Electrodes

—page 179

Biosensing —page 79

Physiology—Epithelial

—page 54

Physiology—Miscellaneous

—page 64

Documents

World Precision Instruments