2-Varian 2005 Sample Prep Web

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Quality and Commitment for Over 25 Years

For more than 25 years, Varian has been solving sample preparation challenges with pioneering techniques and dedicated customer support. Varian introduced the original straight-walled Bond Elut SPE cartridge in 1979 that has now become the standard format for solid phase extraction (SPE). This innovation has continued with the development of novel chemistries and formats that make it simpler, easier, and faster to perform sample preparation. The most recent development is FOCUS, a new SPE technology that provides cleaner extracts and enhanced polar recovery.

Today, Varian has an unparalleled offering of products for sample preparation. Whether you need packed bed or disc; polymer or silica; cartridge, 96-well plate or pipette tip, Varian has the solution for you.

A Continuing History of Innovation

• Bond Elut: the original packed-bed SPE. Over 40 bonded phases are available in a wide range of cartridge formats to meet your sample requirements.

• Chem Elut: the first extraction cartridge to dramatically simplify liquid/liquid extraction. Now available in 96-well format.

• VersaPlate: brings flexibility to 96-well SPE. A modular design with removable tubes eliminates waste and streamlines the method development process.

• Captiva: unique filtration products in 96-well, 3 mL and 10 mL formats fulfill your bioanalysis sample preparation needs, such as filtration of precipitated plasma proteins.

• MetaFlash: high quality Flash Chromatography products provide superior performance and are stocked for fast delivery.

• SPEC: trouble-free SPE. Unique monolithic disc with enhanced flow characteristics is ideal for trouble-free, high-throughput sample processing.

• FOCUS: Polar-Enhanced SPE. Unique new chemistry enhances recoveries of polar analytes and provides cleaner extracts.

• TOP: a simple, cost-effective, high-throughput solution for Trityl-on Oligonucleotide Purification.

Varian Sample Preparation Products

Contact your Varian, Inc. sales representative for a free copy, or use Scanview online at www.varianinc.com, under Consumables, click Applications Online.

GC—LC—MS—AAS—ICP—UV-Vis-NIR—FT-IR—Fluorescence—Dissolution—NMR—Consumable Products

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Sample Preparation Product Selection Guide


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Sorbent Phase Category Bonded Functional Group/ Base Material Endcapped Format

Typical Carbon Loading

Surface aream2/g

Particle Size and Shape

Mean Pore Size

ÅpKa

MeanCapacitymeq/g

AccuCAT Mixed ModeSulfonic acid (SCX) and Quaternary

amine (SAX)/ Silica basedNo

Packed bed

7.0% 50040 and 120 µm, irregular

60

Alumina (AL-A) Polar Aluminum Oxide - acidic N/APacked

bed0% N/A 25

Alumina (AL-B) Polar Aluminum Oxide - basic N/APacked

bed0% N/A 25

Alumina (AL-N) Polar Aluminum Oxide - neutral N/APacked

bed0% N/A 25

Aminopropyl (NH2)

Polar/Anion Exchanger

Aminopropyl/Silica based NoPacked

bed6.7% 500

40 and 120 µm, irregular

60 9.8 1.1

SPEC Aminopropyl (NH2)

Polar/Anion Exchanger

Aminopropyl/Silica based No SPEC Disc 220 N/A 70

AtrazineApplication

SpecificTrifunctional Octadecyl/

Silica basedYes

Packed bed


60

BioBond Elut NonpolarTrifunctional Octadecyl/

Silica basedYes

Packed bed


500

C1 Nonpolar Methyl/Silica based YesPacked

bed4.1% 500

40, irregular

60

C2 Nonpolar Ethyl/Silica based YesPacked

bed5.6% 500


60

SPEC C2 Nonpolar Dimethyl/Silica based No SPEC Disc 2.7% 220 N/A 70

C8 Nonpolar Octyl/Silica based YesPacked

bed12.2% 500


60

SPEC C8 Nonpolar Octyl/Silica based Yes SPEC Disc 5.0% 220

C18 NonpolarTrifunctional Octadecyl/

Silica basedYes

Packed bed


60

SPEC C18 NonpolarMonofunctional Octadecyl/Silica

basedNo SPEC Disc 8.0% 220 N/A 70

SPEC C18 AR NonpolarTrifunctional Octadecyl/

Silica basedYes SPEC Disc 9.0% 220 N/A 70

C18 EWP NonpolarTrifunctional Octadecyl/

Silica basedYes

Packed bed

6.0% 8040 µm,

irregular500

C18 INT NonpolarTrifunctional Octadecyl/

Silica basedYes

Packed bed

13.0% 50040 µm,

irregular60

C18 LO NonpolarTrifunctional Octadecyl/

Silica basedYes

Packed bed

11.8% 50040 µm,

irregular60

C18 OH NonpolarMonofunctional Octadecyl/Silica

basedNo

Packed bed


150

CBACation

ExchangerCarboxylic acid/Silica based Yes

Packed bed


60 4.8 0.4

Certify Mixed ModeOctyl and benzenesulfonic acid (SCX)/

Silica basedNo

Packed bed


60

Certify II Mixed ModeOctyl and quaternary amine (SAX)/

Silica basedNo

Packed bed


60

CH Nonpolar Cyclohexyl/Silica based YesPacked

bed9.6% 500


60

Cyano (CN-E) Nonpolar Cyanopropyl/Silica based YesPacked

bed8.1% 500


60

Cyano (CN-U) Polar Cyanopropyl/Silica based NoPacked

bed7.8% 500


60

SPEC Cyano Polar Cyanopropyl/Silica based No SPEC Disc 220 N/A 70

Sorbent Specifications


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Sorbent Specifications

Sorbent Phase Category Bonded Functional Group/ Base

Material Endcapped FormatTypical Carbon Loading

Surface aream2/g

Particle Size and Shape

MeanPore Size

ÅpKa

MeanCapacitymeq/g

SPEC DAUApplication

specificSilica based SPEC Disc N/A 220 N/A 70

DEA Anion ExchangerDiethylaminopropyl/

Silica basedNo

Packed bed


60 10.7 1.0

Diol (2OH) Polar Diol/Silica based NoPacked

bed6.8% 500

40 µm, irregular

60

ENV Nonpolar Styrene DivinylbenzenePacked

bedN/A N/A

125, spherical

450

EnvirElut 1664

Application Specific

Trifunctional Octadecyl/Silica based

NoPacked

bed18.0% 500


60

FL Polar FlorisilPacked

bedN/A N/A 200 --

FOCUSPolar-enhanced Polar functionalized

styrene divinylbenzenePacked

bedN/A 640

44 µm,spherical

120

LMS Nonpolar Styrene DivinylbenzenePacked

bedN/A N/A

75 µm, spherical

300

SPEC MP1 Mixed ModeNonpolar and benzenesulfonic acid

(SCX)/Silica basedSPEC Disc 6.0 220 N/A 70

SPEC MP3 Mixed ModeSlightly polar and benzenesulfonic

acid (SCX)/Silica basedSPEC Disc 220 N/A 70

NEXUS Mixed mode Mixed Mode CopolymerPacked

bedN/A 575

70 µm, spherical

100/450 Bimodal

PBA Covalent Phenylboronic acid NoPacked

bed7.9% 500

40 µm, irregular

60

PCBApplication

SpecificLayered Phase N/A

Packed bed

N/A 500 N/A N/A

PH Nonpolar Phenyl/Silica based YesPacked

bed10.7% 500


60

SPEC PH Nonpolar Phenyl/Silica based Yes SPEC Disc 220 N/A 70

PPL Nonpolar Functionalized Styrene Divinylbenzene N/APacked

bedN/A 600

125 µm, spherical

150

PRS Cation ExchangePropylsulfonic acid/

Silica basedNo

Packed bed

1.7% 50040 µm,

irregular60 <1.0 0.18

PSA Anion Exchanger Ethylenediamine-N-propyl/Silica based NoPacked

bed7.5% 500


6010.1&

10.91.40

SPEC PSA Anion Exchanger Ethylenediamine-N-propyl/Silica based No SPEC Disc 220 N/A 70

SAX Anion ExchangerTrimethylaminopropyl/

Silica basedNo

Packed bed


60Quater-

nary 0.85

SPEC SAX Anion ExchangerTrimethylaminopropyl/

Silica basedNo SPEC Disc 220 N/A 70

SCX Cation ExchangerBenzenesulfonic acid/

Silica basedNo

Packed bed


60 <1.0 0.79

SPEC SCX Cation ExchangerBenzenesulfonic acid/

Silica basedNo SPEC Disc 220 N/A 70

SI Polar Silica NoPacked

bedN/A 600


60

SPEC SI Polar Silica No SPEC Disc 220 N/A 70

TCAApplication

SpecificEthyl/Silica based Yes

Packed bed


60

TOPApplication

SpecificFunctionalized Styrene Divinylbenzene

Packed bed

N/A 600125 µm, spherical

150


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Sorbent Selection Guide

AccuCATBond Elut AccuCAT cartridges are mixed-mode SPE columns consisting of a strong cation exchange and a strong anion exchange sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic, and neutral compounds from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from biofluids.

Alumina-A (AL-A)• Acidic Alumina (Al2O3) • pH: 4.5• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion exchange

Alumina is a classic Lewis acid, lacking two electrons in the aluminum center. The Lewis acid properties are enhanced in Alumina-A, making the sorbent more retentive towards electron-rich compounds. This sorbent has a slightly cationic nature through pretreatment with acidic solutions. The result is a surface that favors neutral and anionic species (e.g. neutral acid or acid anion), while discouraging retention of cationic species. Weak ion exchange is also possible with anionic compounds.

Alumina-B (AL-B)• Basic Alumina (Al2O3)• pH: 10.0• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion-exchange

Alumina-B has a surface that favors retention of cationic or hydrogen-bonding species. This sorbent has an anionic nature through the pretreatment of the alumina with basic solutions. The result is a surface that favors cation exchange, though the surface also exhibits some Lewis base properties and therefore can also retain electron-donors like neutral amines. However, this capacity is much lower than for the neutral and acidic aluminas. Strong hydrogen bonding is possible on Alumina-B, making it particularly effective for polar cations.

Alumina-N (AL-N)• Neutral Alumina (Al2O3)• pH: 7.5• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion exchange

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The smaller particle size ensures high extraction efficiency and small (50 mg) sorbent beds are therefore commonly used. This sorbent has an electrically neutral surface that favors the retention of electron-rich compounds like aromatic species and aliphatic amines. It also favors retention of compounds containing electronegative groups (for example, functional groups with oxygen, phosphorus and sulfur atoms). It may be used in extracting either nonpolar or polar species from aqueous or nonaqueous samples respectively.

Aminopropyl (NH2)• Ideal for structural isomer fractionation• Anion exchange and polar retention mechanisms• Weak nonpolar retention

Aminopropyl (NH2), a polar sorbent, utilizes both hydrogen bonding and anion exchange. With a pKa of 9.8, NH2 is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged) and is therefore a better sorbent choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on SAX. Because a propyl tether supports the NH2 functionality, it can be used for nonpolar isolations from polar samples, but its strong polarity is its primary characteristic. NH2 is excellent for separation of structural isomers.

AtrazineBond Elut Atrazine is specially designed for the extraction of Atrazine and its desalkylated metabolites -- des-ethylatrazine and desisopropylatrazine -- from water samples. The sorbent is based on Bondesil C18-LO, with carefully controlled carbon content to ensure reproducibly high recoveries of Atrazine and its polar metabolites. The bigger particle size of the sorbent and the diameter of the tube allow fast flow for large volume water samples. And, the large bed mass of the Bond Elut Atrazine phase has been optimized to provide enough capacity to ensure good recovery of Atrazine and its polar metabolites. With the use of the Bond Elut Atrazine extraction cartridge and optimized method (M2638) in combination with a Varian HPLC diode array detection system, quantitative recoveries of Atrazine and its desalkylated metabolites in surface water have been achieved, even at extremely low concentrations (0.02 ppb). These concentration levels are far below the tolerance level specified by the EPA (3 ppb) and by those set by the most stringent European agencies (0.1 mg/L and 0.5 mg/L). The extraction and analysis methods are fast, reproducible, and require minimal organic solvent consumption.

BioBond ElutChoose BioBond Elut for fast, simple desalting of synthetic oligonucleotides from final deprotection reaction mixtures. With BioBond Elut there are no minimum molecular weight limits, so oligos less than 5,000 Daltons can be desalted with >98% recoveries. And, while typical desalting media can leave as much as 2% of the original salt in your sample, BioBond Elut provides 100% salt-free samples.


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C1• Bonded functional group: methyl• Primary retention mechanism: very weakly nonpolar• Typical sample types: plasma, urine, and aqueous samples

Due to the single carbon functional group, Bond Elut C1 is the least retentive of all alkyl-bonded phases for nonpolar compounds. However, because the sorbent is endcapped, silanol activity is generally masked. The result is easier retention and elution of polar and multifunctional compounds.

C2 • Bonded functional group: ethyl• Primary retention mechanism: weakly nonpolar• Typical sample types: plasma, urine, and aqueous samples

Bond Elut C2 is generally considered more polar than longer-chain alkyl-bonded sorbents. The short C2 alkyl group provides a smaller area for nonpolar interactions to occur. C2 is often used as a replacement for C18 and C8 when molecules are retained too strongly by the latter. C2’s polarity is slightly lower than CN for polar interactions. It is popular for the extraction of drugs from plasma and serum because of the very flat chromatographic baselines that can be achieved with appropriate wash and elution conditions.SPEC C2 is a monolithic disk featuring an unendcapped dimethyl bonded functional group. SPEC C2, therefore, offers a different selectivity than Bond Elut C2.

C8• Bonded functional group: octyl• Primary retention mechanism: moderately nonpolar• Typical sample types: water, aqueous biological samples

Bond Elut C8 is very similar in properties to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain. C8 is a good replacement for C18 when analytes are too strongly retained on C18 for effective elution. The potential of C8 for polar interactions with analytes is somewhat higher than for C18 because the shorter hydrocarbon chain does not mask the silica surface as effectively, but polar interactions are still not a significant property of C8. The C8 sorbent has been successfully utilized in the simultaneous extraction of fat- and water-soluble vitamins from human serum.

SPEC C8 offers similar selectivity to Bond Elut C8.

C18• Bonded functional group: trifunctional octadecyl• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids

Bond Elut C18 is the most hydrophobic silica-based sorbent available. It is the most popular SPE sorbent because of its extreme retentive nature for non-polar compounds. C18 is generally regarded as the least selective silica based sorbent, because it retains most organic analytes from aqueous matrices — often a benefit when the analytes vary widely in structure. The potential for polar interactions with analytes is less significant with C18 than with any other sorbent because of the predominant effect of the long hydrocarbon chain. When extracting small- to intermediate-sized molecules, C18 can also be utilized for desalting prior to ion exchange because salts pass through it unretained.SPEC C18 is a nonendcapped, monofunctional version of the octadecyl bonded phases and offers similar selectivity to Bond Elut C18-OH.

C18 AR• Bonded functional group: trifunctional octadecyl• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids

SPEC C18AR (acid resistant) is an endcapped, trifunctional version of the octadecyl phases and offers similar selectivity to Bond Elut C18. C18AR is exclusively available in SPEC disc format.

C18 EWP• Bonded functional group: trifunctional octadecyl• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids

Bond Elut C18 EWP (extra wide pore) sorbent has 500 Å pores to allow more efficient extraction of large molecules (> 15,000 MW). Macromolecules of this size are typically excluded from standard porosity phases, but with C18 EWP successful separation of complex mixtures of proteins, peptides or nucleotides is achieved. C18 EWP is a good sorbent for desalting large molecular weight compounds prior to LC/MS or LC/MS/MS, capillary electrophoresis, or any other analytical technique that is adversely affected by the presence of salts in the sample.



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C18 INT• Bonded functional group: trifunctional octadecyl• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids

Bond Elut C18 INT (intermediate carbon loading) is similar in properties to the standard Bond Elut C18 sorbent, but has a slightly lower carbon loading. This intermediate carbon load C18 is thoroughly endcapped, ensuring rapid release of basic drugs. The carbon loading has been optimized to differentiate between metabolites of classes of compounds such as prostaglandins. In addition, the narrow pore size helps exclude large molecules such as proteins.

C18 LO• Low carbon loading gives unique C18 selectivity• Strongly nonpolar retention mechanism• Ideal for polar analyte extraction from water samples and

biological fluids

C18 LO, like C18 and C18 INT, is a highly retentive nonpolar silica-based sorbent due to the long hydrocarbon chain. However, the low carbon loading of C18 LO gives unique selectivities over other octadecyl sorbents. The carbon loading has been optimized for polar analyte extraction from environmental matrices. Quantitative recoveries of Atrazine metabolites have been successfully achieved with C18 LO sorbents. (Note: see Bond Elut Atrazine section for optimized bed mass and application information.)

C18 OH• Bonded functional group: monofunctional octadecyl• Primary retention mechanism: moderately nonpolar, plus significant

polar secondary interactions• Typical sample types: biological matrices, aqueous samples

Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded phases that enable the silanols on the silica surface to be more active. This low-load C18 has controlled silanol activity under tight QC specifications for superior batch-to-batch reproducibility. The silanol activity permits fractionation of metabolites and enhances retention of basic compounds compared with endcapped C18. In addition, the 150Å intermediate pore size is ideal for intermediate molecular weight compounds that might not be retained on a standard 60Å material.SPEC C18 offers similar selectivity to Bond Elut C18 OH.

Carboxylic Acid (CBA)• Bonded functional group: carboxylic acid• Primary retention mechanism: weak cation exchange• Best choice for extraction of strong cations• Typical sample types: water, biological fluids

Bond Elut CBA is a medium polarity sorbent based on carboxy-propyl-functionalized silica, and exhibits either polar or nonpolar interactions depending on the matrix or solvent environment. Its primary characteristic is its weak cation exchange ability. Because of its pKa of 4.8, CBA does not require the high basic conditions for elution that are required by strong cation exchange sorbents. Simply, lowering the pH to 2.8 effectively neutralizes CBA, permitting elution of the retained analytes. For this reason, CBA is often the best choice for extraction of very strong cations.

Certify• Bonded functional group: mixed-mode sorbent bed, octyl (C8) and

benzenesulfonic acid (SCX)• Primary retention mechanism: nonpolar and strong cation exchange• Ideal for forensic and drugs of abuse analyses• Typical sample types: urine, biological fluids

Bond Elut Certify is the original mixed-mode sorbent, designed for the extraction of basic (cationic) drugs from urine, plasma, serum, whole blood and other biological matrices. Examples of drugs and drug classes that can be extracted with Certify include:• Basic and nonpolar pharmaceutical compounds• HC metabolite, 11-nor-delta-9-tetrahyrdocannabinol-9- carboxylic acid• Cocaine and benzoylecognine• Amphetamine and methamphetamine• Phencyclidine• Opiate and 6-MAM• Barbiturates• Benzodiazepines• Acid/base/neutral screens

Analytes are strongly retained on Certify by a combination of ion exchange and nonpolar mechanisms. This allows the use of aggressive aqueous and organic washes to rinse away nonpolar, polar and ionic matrix contami-nants. The result is outstanding sample clean up and concentration of samples prior to screening or confirmation by GC, GC/MS, LC/MS or TLC.

SPEC MP-1 offers similar selectivity to Bond Elut Certify.



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Certify II• Bonded functional group: mixed-mode sorbent bed; octyl and

quaternary amine (SAX)• Primary retention mechanism: nonpolar and strong anion exchange• Typical sample types: urine, biological fluids

Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and strong anion exchange (SAX) sorbent. It has been optimized for extraction of acidic and nonpolar drugs such as THC metabolite and nonsteroidal anti-inflammatory drugs.

Cyclohexyl (CH)• Bonded functional group: cyclohexyl• Primary retention mechanism: moderately nonpolar• Typical sample types: water, aqueous samples

Bond Elut CH is a medium polarity sorbent that exhibits unique selectivity in nonpolar extractions. When employed as a nonpolar sorbent, CH has the approximate polarity of a C2 sorbent. The polar subsurface is an important factor among CH properties, as indicated by the ability of CH to retain polar analytes such as phenol from aqueous matrices. Because of its unique selectivity, CH is often a good choice when other nonpolar sorbents do not provide the desired selectivity.

Cyanopropyl (CN-E)• Bonded functional group: endcapped cyanopropyl• Primary retention mechanism: moderately nonpolar (aqueous matrix)

or polar (nonpolar organic matrix)• Typical sample types: aqueous (nonpolar), organic (polar)

Bond Elut CN-E is a medium polarity sorbent ideal for extraction of extremely nonpolar compounds that irreversibly retain on more nonpolar sorbents such as C18 or C8. The endcapped version of the cyano sorbent is best utilized when extracting analytes from an aqueous matrix.

Cyanopropyl (CN-U)• Bonded functional group: unendcapped cyanopropyl• Primary retention mechanism: weakly nonpolar (aqueous matrix) or

polar (nonpolar organic matrix)• Typical sample types: nonpolar solvents, organic extracts of aqueous

sample

Unendcapped Bond Elut CN-U is a medium polarity sorbent. It is less retentive than Si or 2OH for very polar analytes that might be retained irreversibly on these more polar sorbents. The unendcapped cyano phase is ideally suited for the extraction of polar compounds from a nonpolar matrix such as hexane or oils.

SPEC CN offers similar selectivity to Bond Elut CN-U.

DAUSPEC DAU is specifically designed for the analysis of Drugs of Abuse in urine. Based on the monolithic silica extraction disc, SPEC DAU is ideally suited for high-throughput automation. Its unique sorbent chemistry results in outstanding sample cleanup and concentration of samples prior to screening or confirmation by GC, GC/MS, LC/MS or TLC.

Diethylaminopropyl (DEA)• Bonded functional group: diethylaminopropyl• Primary retention mechanism: weak anion exchange aqueous matrix)

or polar (nonpolar organic matrix)• Typical sample types: water, biological fluids, nonpolar extracts

Bond Elut DEA bears some resemblance to PSA and NH2 in its properties. It has slightly lower capacity as an anion exchange sorbent (1.0 meq/g), and a more nonpolar character due to a longer alkyl tether. DEA is somewhat more polar than C8, but less polar than C2 or CN.

Diol (2OH)

• Polar sorbent with better reproducibility than silica• Ideal for prostaglandin separation and other fractionations• Typical sample types: nonpolar organic extracts, oils, and lipids

Diol (20H) is typically used for polar extractions from nonpolar solvents. Diol resembles unbonded silica in its tendency for strong hydrogen bonding. It also shares silica’s important ability to discriminate between similar compounds such as structural isomers. Prostaglandins, for example, can be separated into classes using Diol by slightly varying the solvent polarity. Diol is a more predictable sorbent than silica for extraction methods requiring a polar sorbent conditioned with a polar solvent. Diol can also be used for nonpolar retention by virtue of its hydrocarbon spacer.



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ENV• Sorbent description: polymeric wide-pore SDVB• Typical applications: environmental, explosives, pharmaceuticals

Bond Elut ENV is a high purity styrene-divinylbenzene (SDVB) polymer optimized for the extraction of polar organic residues, such as herbicide metabolites and explosives, from large volume water samples. Bond Elut ENV features a 125 µm highly cross-linked spherical polymer that can process 1 L water samples in a fraction of the time required by other sample preparation methods. The absence of secondary interactions on the polymer surface makes it easier to elute a wide range of nonpolar compounds with just one elution solvent. Although primarily used for environmental applications, Bond Elut ENV can also be used for clinical applications, including the extraction of highly polar metabolites, such as glucuronide-bound molecules from biological fluids.

EnvirElut 1664• Specifically designed for EPA Method 1664 - Oil and Grease extraction

from water• High-flow - 1 L of sample in less than 10 minutes• Manifold simplifies sample loading

EnvirElut 1664 offers a simple, rugged and cost effective solution for the processing of oil and grease samples. EnvirElut cartridges consistently pass 1 liter of dirty water in less than 10 minutes, without loss or analyte, and deliver greater than 90% recovery with standards from 5 to 1,000 mg/L. The procedure is totally Freon - free. When combined with Varian’s AquaSep cartridges for moisture removal, EnvirElut 1664 offers a solid phase- extraction procedure with results equivalent to EPA 1664/ASTM method 413.1 (gravimetry).

Florisil• Sorbent description: MgO3Si• Primary retention mechanism: polar• Typical sample types: environmental, organic extracts

Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for the isolation of polar compounds from nonpolar matrices. The larger particle size – 200 µm – of the Florisil material enables fast flow of large-volume samples and thus is a potential alternative to silica when extracting viscous samples. Florisil is ideal for the separation of chlorinated pesticides, and is used in numerous AOAC- and EPA- regulated methods. Florisil has also been used as a replacement for Alumina in cases where the Lewis-acidity of alumina interferes with the extraction.

FOCUS• Sorbent description: polar-enhanced SDVB polymer• Primary retention mechanism: polar and nonpolar• Power Rinse™ delivers cleaner extracts/reduced ion suppression• Typical sample types: pharmaceutical bioanalysis

FOCUS features a polar-enhanced sorbent technology that delivers outstanding recovery for polar as well as nonpolar analytes. Compared to other polymeric sorbents, FOCUS exhibits a unique hydrogen bond donor capability that significantly increases the retention of basic analytes. This increased retention results in cleaner extracts and higher recoveries across a broad spectrum of drugs. The extraction method is simple, universal, and eliminates the complex pH adjustments necessary with ion exchange and mixed mode methods.

LMS• Sorbent description: highly cross-linked SDVB polymer• High, controlled surface area for consistent extraction efficiency• Typical sample types: biological samples such as urine, plasma, or serum

Bond Elut LMS allows elution without organic modifiers, buffers, or acids. The absence of secondary silanol interactions allows elution of analytes with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics ensure compatibility with LC/MS and other analytical techniques. Featuring an optimized particle size range and a large surface area for extraction efficiency, Bond Elut LMS provides excellent recovery and reproducibility. LMS ensures worry-free use and can help reduce costly instrument downtime.

MP1• Bonded functional group: mixed-mode SPEC disc: nonpolar/

benzenesulfonic acid (SCX)• Primary retention mechanisms: nonpolar and strong cation exchange• Typical sample types: urine, biological fluids

SPEC MP-1 is a mixed-mode, monolithic disc format sorbent that delivers superior flow and reproducible recoveries. SPEC MP-1 is ideal for analyzing drugs with polar functional groups from plasma using LC/MS/MS. The dual retention mechanism results in cleaner extracts. During the extraction, polar basic drugs are strongly held by the benzenesulfonyl group while rigorous wash steps can be applied to remove the matrix components that cause ion suppression.

Bond Elut Certify offers similar selectivity to SPEC MP-1.

MP3• Bonded functional group: mixed-mode SPEC disc: slightly polar/

benzenesulfonic acid (SCX)• Primary retention mechanism: slightly polar and strong cation

exchange• Typical sample types: urine, biological fluids

SPEC MP-3 is slightly more polar than MP-1, making MP-3 ideal for hydrophobic analytes that would bind too strongly to MP-1. MP-3 is especially suited for the extraction of opiate alkaloids.



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NEXUS• Unique, ultraclean copolymeric material• No conditioning needed for hydrophobic analytes• High flow for viscous samples• Ideal for veterinary sciences and environmental applications

Nexus provides simple, clean SPE on a unique polymeric sorbent. It offers a nonpolar retention mechanism with no conditioning required, enabling increased throughput or improved ease of field sampling in environmental applications. Nexus delivers reproducibly high recoveries. This performance is achieved from the use of a proprietary, highly cross-linked, spherical polymeric sorbent that can bind both polar and nonpolar analytes. The large particle size makes Nexus ideal for extracting highly viscous samples such as horse urine.

PCBBond Elut PCB is tailor-made to extract a wide range of polychlorinated biphenyls (PCBs) from transformer oil and other organic matrices. Bond Elut PCB is a multiphase cartridge that consists of a charged polar surface packed in an ultraclean 3 mL polypropylene tube. Using Bond Elut PCB and optimized method (M2647), quantitative recoveries of a wide range of PCB compounds have been obtained using a simple one-solvent procedure. When Bond Elut PCB is used in combination with the CP-Select 28/31 GC capillary column (Part Number CP7479), a wide range of PCB compounds, including PCB 28 and 31, can be isolated and resolved in half the time of other SPE and GC procedures

Phenylboronic Acid (PBA)• Strong covalent retention mechanism• Multiple secondary interactions• Ideal for cis-diol extraction

PBA (Phenylboronic Acid) is a unique sorbent consisting of a phenylboronic acid bonded to a silica gel surface. The PBA sorbent covalently binds cis-diol, cis-amino alcohols, and other related functional groups. Because covalent retention involves an interaction of 10-100 times greater energy than other extraction mechanisms, compounds are more tightly bound to the sorbent surface, resulting in superior clean-up. The boronate group has a high specificity for cis-diol containing compounds such as catechols, nucleic acids, low molecular weight proteins and carbohydrates. PBA is especially effective in the isolation of catecholamines from biological fluids and for the separation of RNA from DNA. It may also be applied to cis-amino alco-hols such as propranolol. Retention is usually strongest when the analytes’ functional groups are coplanar.

Phenyl (PH)• Bonded functional group: phenyl• Primary retention mechanism: moderately nonpolar• Typical sample types: water, biological fluids

Bond Elut PH is most commonly employed for nonpolar extractions, with a similar polarity to a C8 sorbent. Like cyclohexyl, PH exhibits a slightly different selectivity from other nonpolar sorbents. This added selectivity results from the electron density of the aromatic ring. Retention of planar, conjugated organic molecules is enhanced compared to the aliphatic bonded phases.

SPEC PH offers similar selectivity to Bond Elut PH.

PPL• Sorbent description: functionalized SDVB polymer• High surface area for excellent retention of polar analytes• Typical sample types: phenols from waste water

Bond Elut PPL, a functionalized styrene-divinylbenzene polymer, has been optimized for the extraction of highly polar species from large-volume water samples. Featuring a proprietary high-purity spherical polymer with an extremely nonpolar surface, Bond Elut PPL retains even the most polar compounds, such as phenols, and achieves high recoveries and fast extraction speeds.and fast extraction speeds.

Propylsulfonic Acid (PRS)• Bonded functional group: propylsulfonic acid• Primary retention mechanism: strong cation exchange• Typical sample types: water, biological samples

Bond Elut PRS is a strong cation exchange sorbent that is also very polar. As such, PRS exhibits weak nonpolar interactions. The pKa of PRS is low, and cationic analytes must be eluted either by high ionic strength, or by neutralizing the charge on the cationic analyte. Therefore, PRS is most effective for weaker cations such as pyridinium compounds. Also, PRS is recommended where nonpolar secondary actions of SCX interfere with the extraction.



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PSA• Bonded functional group: ethylenediamine-N-propyl (primary and

secondary amines)• Primary retention mechanism: weak anion exchange (aqueous matrix)

or polar (nonpolar organic matrix), chelation• Typical sample types: water, biological fluids, and organic extracts

Bond Elut PSA is an anion exchange sorbent that is similar to NH2. PSA has two amine groups that offer slightly greater ionic capacity (1.4 meq/g) than NH2 (1.1 meq/g), and the pKas of its primary and secondary amine groups are higher (10.1 and 10.9, respectively). PSA is therefore a stronger anion exchanger than NH2. The PSA functional group is also a very good bidentate ligand, making PSA an excellent sorbent for chelation applications (useful for metal ion extractions). Its higher carbon loading makes it a less polar sorbent than NH2, and thus a better choice for very polar compounds that retain too strongly on NH2 sorbent.SPEC PSA offers similar selectivity to Bond Elut PSA.

SAX• Strongest anion exchanger available• Quaternary ammonium chloride for intermediate selectivity

SAX (Strong Anion eXchange) is the strongest anion exchange sorbent available. Because its functional group is a quaternary amine, the sorbent is always charged. SAX offers minimal nonpolar interactions because any effect of the carbon atoms in the functional group is masked by the amine. Because SAX is such a strong anion exchanger, it is a good sorbent for retention of weaker anions such as carboxylic acids that may not retain strongly enough on weaker anion exchange phases. SAX contains a chloride counterion. The user can modify the selectivity of SAX by conditioning with appropriate buffers, exchanging chloride for ions of greater or lesser selectivity.

SCX• Bonded functional group: benzenesulfonic acid• Primary retention mechanism: strong cation exchange• Typical sample types: aqueous, biological fluids and organic reaction

mixtures

Bond Elut SCX is a strong cation exchanger with a very low pKa, and is similar to PRS as an ion exchange sorbent. The SCX sorbent has a higher potential for nonpolar interactions than PRS due to the presence of the benzene ring. This potential for nonpolar interactions should be considered when the sorbent is used for ion exchange from aqueous samples. This dual nature is useful for extracting compounds that exhibit both cationic and nonpolar character. The overall result of the dual character is superior clean up using only a single sorbent.SPEC SCX offers similar selectivity to Bond Elut SCX.

Silica (SI)• Sorbent description: native silica, unfunctionalized• Primary retention mechanism: strongly polar• Typical sample types: nonpolar organic solvents, oils, and lipids

Silica is generally regarded as the most polar sorbent available. Unbonded, activated silica is also fairly acidic. SI is one of the best sorbents available for selectively separating compounds of very similar structure. Applying the analytes in a nonpolar solvent, then gradually increasing the solvent polarity in successive wash steps by adding increasing concentrations of polar modifiers, such as THF or ethyl acetate, can accomplish this separation.

Bond Elut SI and SPEC SI offer similar selectivity.

TCA• Optimized for tricyclic antidepressant (TCA) extraction• Broad concentration range (20-2000 ng/mL)

TCA is a nonpolar sorbent optimized for extraction of tricyclic antidepressants (TCAs). Each lot of Bond Elut TCA is specifically quality tested for recovery of TCAs, ensuring reproducibly good performance.

TOPTOP (Trityl-on Oligo Purification) was created with a very high selectivity for trityl-on oligonucleotides. It is based on a unique functionalized styrene divinylbenzene material that allows direct loading of the basic deprotection solution. This ensures that all the full-length oligonucleotide product will bind the first time it is passed through the TOP cartridge. The sample - sorbent interaction is further maximized through the controlled particle size distribution of the TOP resin. Also, the large particle size resin permits gravity flow operation, eliminating the cost of vacuum manifolds. The result is high, reproducible yields and a simplified, more robot-friendly process.


OMIX Pipette Tips


12

OMIX® Pipette Tips• Extract drugs, peptides and proteins• Purify 10-100 uL samples• Elute with 0.5-100 uL• Directly shoot into the LC/MS/MS or spot onto a MALDI plate

Solutions for Microvolume SPE

Monolithic sorbent tip technology for dependable purification and superior results in Drug Discovery and Proteomix research.

OMIX pipette tips (patent pending) for microextractions reliably purify and enrich femtomole to micromole levels of small molecule drugs, peptides and proteins prior to MALDI-TOF or LC/MS. The unique monolithic sorbent technology used in OMIX consistently outperforms other microvolume techniques by delivering uniform flow and strong analyte-to-surface interactions for consistent, high recoveries.

OMIX tips in Proteomix research provide:• Improved Precision: Achieve up to a 8-fold improvement in RSDs with OMIX monolithic technology• Increased Recoveries: Isolate up to 50% more peptide with OMIX’s superior binding capacity• Better MASCOT Results: Obtain higher MASCOT scores, percent sequence coverage, and number of peptides matched for more reliable protein identification• Higher Peak Intensities: Assure correct protein identification with significant signal improvements• Greater Confidence: Experience dependable tip performance and prevent precious sample loss

OMIX tips in Pharmacokinetics studies provide:• Freedom to work with limited amounts of sample: 5 to 200 µL rat or mouse plasma• Greater sensitivity through low elution volumes: less than 50 µL• Faster sample prep with direct injection into an LC/MS/MS

OMIX tips contain a C18 or a C4 reversed phase sorbent packed into in 10 or 100 µL pipette tips. The C18 phase is recommended for small molecules and peptides, while the C4 phase is recommended for proteins. The 100 µL volume tips are ideal for small molecule applications and larger proteolytic digests (greater than 5µL) where very high capacity is required (up to 80 µg) and elution volumes greater than 5 µL are acceptable. For proteolytic digests less than 10 µL in volume, use either the standard OMIX C18 tips for intermediate capacity and elution volumes, or use OMIX C18-MB (minibed) tips for ultra-low elution volumes.

OMIX Pipette TipsSorbent Phase

Elution Volume 8 /Pk 8 /Pk 96 /Pk 96 /Pk 576 /Pk 576 /Pk

C18

0.5-2 µL A57003MBSPL A57003MB A57003MBK 2-10 µL A5700310SPL A5700310 A5700310K 5-100 µL A57003100SPL A57003100 A57003100K

C4

0.5-2 µL A57009MBSPL A57009MB A57009MBK 2-10 µL A5700910SPL A5700910 A5700910K 5-100 µL A57009100SPL A57009100 A57009100K

TOP Trityl-on Oligon Purifi cation


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TOP™ (Trityl-on Oligon Purification)• High throughput, gravity flow oligonucleotide purification• Purify 96 oligos in 45 minutes• Robot compatible for automated, unattended operation• Generate reproducible yields with gravity flow• Eliminate multiple loading steps• Directly load the ammonia or AMA onto the resin• Save up to 12 hours of evaporation time

TOP meets the demand for simple, cost effective, high throughput purification of trityl-on oligonucleotides. TOP incorporates a unique 96-well plate with removable tubes, streamlined gravity flow protocol, polymeric resin and optimized binding solution. Varian’s cutting edge BioSolutions technology delivers superior yields and purity for standard oligos up to 1 umol in scale and up to 50 mer in length.

96-well plates: Up to 200 nmol scale. TOP will purify oligonucleotides up to 200 nmol in scale using the 96-well plate format and either the 25 or 50 mg bed mass. These tubes are available in the following packaging options:

Starter Kits: Simplify the ordering process with two prepackaged solutions. The kit includes a Preassembled 96-well Plate, TOP Buffer Pak, Collection Plate, Disposable Waste Tray, Well Plate Tube Removal Tool and TOP User Guide. Each kit will purify up to 96 oligos and is recommended for first-time users.

Preassembled Plates: Complete 96-well plates minimize assembly time and labor.

Well Plate Tubes: Order either 96 or 24 packs of well plate tubes with the reusable base plate to reduce the overall cost per oligo while maintaining the 96-well footprint. Two additional bed dimensions are available in the preassembled Plate and the Well Plate Tube options - a 100 mg bed for applications that require higher capacity and a 150 mg bed for those customers that prefer vacuum.

Cartridges: Up to 1 umol scale. A standard 6 mL cartridge with 300 or 500 mg of TOP material is available for synthesis scales up to 1 umol. This configuration is the preferred choice for users that do not require high throughput.

TOP buffers: Perfect for reducing solution preparation time and ensuring improved reproducibility. Each case of the quality controlled TOP BufferPak will purify over 1200 oligos and contains both the Binding Buffer and the TEAA Solution. Individual cases of the each buffer type are also available.

For additional information, visit www.varianinc.com/biosolutions/

Starter KitsThe kit includes a Preassembled 96-well Plate, TOP Buffer Pak, Collection Plate, Disposable Waste Tray, Well Plate Tube Removal Tool and TOP User Guide.

Item Name 25 mg 50 mg Volume(mL) Quantity

TOP Starter Kit for 50 nmol Scale 76009025 1.8 1 Kit

TOP Starter Kit for 200 nmol Scale 76009050 1.8 1 Kit

Preassembled 96-well PlatesMax. Synthesis

Scale(nmol)

25 mg 50 mg 100 mg 150 mg Volume(mL) Quantity

50 75619025 1.8 1

200 75619050 7561901C 7561915C 1.8 1

TOP Trityl-on Oligon Purifi cation


14TOP Well Plate Tubes

Max. Synthesis Scale(nmol)

Quantity 25 mg 50 mg 100 mg 150 mg Volume(mL)

5024 /Pk 75819025 1.8

96 /Pk 75719025 1.8

20024 /Pk 75819050 7581901C 7581915C 1.8

96 /Pk 75719050 7571901C 7571915C 1.8

Preassembled 96-well PlatesMax. Synthesis

Scale(nmol)

25 mg 50 mg 100 mg 150 mg Volume(mL) Quantity

50 75619025 1.8 1

200 75619050 7561901C 7561915C 1.8 1

CartridgesMax. Synthesis

Scale(nmol)

300 mg 500 mg Volume(mL) Quantity

1000 12102300 12102301 6.0 30

TOP Prepackaged SolutionsItem Name Part No. Quantity

TOP BufferPak, 950 ml 1x Binding Solution, 3x950 ml 0.1M TEAA Solution 79100000 1 Kit

TOP 1x Binding Solution, 4x 950 ml 79100001 4 /Pk

TOP 2x Binding Solution, 950 ml 79100003 1 Each

TOP 2x Binding Solution, 4x950 ml 79100004 4 /Pk

TOP 0.1M TEAA Solution, 4x950 ml 79100002 4 /Pk

TOP 2M TEAA Solution, 4x950 ml 79100005 4 /Pk

AccessoriesItem Name Part No. Quantity

Disposable Waste Tray 5133001 25 /Pk

96-well Plate Sealing Mat 5133005 50 /Pk

Well Plate Tube Removal Tool 12236107 1 Each

TOP Reusable Baseplate 75400001 1 Each

TOP User Guide 391491900 1 Each

96-Well Collection Plate, 2 ml Well Volume WA77015200 25 /Pk

96-Well Collection Plate, 750 ul Well Volume WA77015750 25 /Pk

FOCUS Polar-Enhanced SPE


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FOCUS™ Polar-Enhanced SPE• Unique polar-enhanced SPE phase• Power Rinse™ delivers cleaner extracts• Reduced ion suppression• Simple, universal method

FOCUS features a unique polar-enhanced sorbent technology that delivers outstanding recovery for polar as well as non-polar analytes. Unlike other polymeric sorbents, FOCUS exhibits hydrogen bond donor capability that significantly increases the retention of basic analytes. This increased retention results in cleaner extracts and higher recoveries across a broad spectrum of drugs. The extraction method is simple and eliminates the complex pH adjustments necessary with ion exchange and mixed mode methods. FOCUS is available in 96-well plate and cartridge formats.

Compare FOCUS to today’s most popular SPE technology

An independent pharmaceutical lab performed a rigorous study to evaluate the performance of FOCUS. The results were then compared to two popular polymeric SPE products. In the end, FOCUS clearly outperformed the competition with cleaner extracts and better recovery over a broad spectrum of drugs (below).

FOCUS Polar-Enhanced SPE


16Cleaner extracts reduce ion suppression

FOCUS’ improved retention for polar analytes allows use of a strong Power Rinse without causing drug loss. Rigorous washes using 10-20% acetonitrile and methanol can be used to aggressively remove endogenous matrix interferences. For very polar analytes that are held by strictly polar interactions, e.g. atenolol, washes of up to 100% can be used. FOCUS produces substantially cleaner extracts compared to alternatives, leading to reduced ion suppression.

FOCUS 96-well SPESorbent Mass

(mg) Part No. Quantity

10 A59660 1 Each

FOCUS CartridgesSorbent Mass

(mg) 1 mL 3 mL 6 mL Quantity

10 A5106010 100 /Pk

20 A5306021 100 /Pk

50 A5606050 100 /Pk

SPEC Disc SPE


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SPEC® Disc SPE• Trouble-free automation• Highly reproducible flow and recoveries• Faster sample processing

Using an advanced disc design, SPEC delivers superior flow characteristics and trouble-free automation.

Low elution volumes mean that evaporation and reconstitution steps may be eliminated, resulting in faster sample processing. By combining low bed mass, ultraclean materials, and optimized selectivity, SPEC delivers higher recoveries free of the matrix interferences that cause ion suppression. SPEC is available in 96-well plate, cartridge, and pipette tip formats. For information on available chemistries, refer to the Sorbent Selection Guide on pages 5-11.

SPEC is available in both cartridge and 96-well formats.

SPEC improves flow and eliminates plugging

When combined with an integral anticlogging device, SPEC’s unique monolithic disc technology achieves highly uniform and reproducible flow even when processing highly particle-laden samples. This results in highly reproducible recoveries and fast, trouble-free automation.

SPEC 96-Well Plates offer outstanding flow characteristics. When used with an automated platform, flow across all 96 wells is uniform and highly reproducible.

Lower elution volumes mean higher productivity

SPEC delivers high recoveries at low elution volumes - as low as 100 µL. This is due to the high surface area of the SPEC monolithic silica disc that results in a very high extraction efficiency. By reducing elution volumes, samples can be directly injected on LC/MS without evaporation and reconstitution while still achieving required LLOQs.

SPEC 96-well Plates• Monolithic silica extraction disc • Trouble-free automation• Highly reproducible flow and recoveries• Faster sample processing

Using an advanced disc design, SPEC delivers superior flow characteristics and trouble-free automation

For a description of these sorbents, see the Sorbent Selection Guide on pages 5-11.

Method Development KitThe SPEC Method Development kit provides a range of sorbents to help you optimize your sample preparation method.

Sorbent Phase 15 mg QuantityC2, C8, C18, C18AR, CN, MP1, MP3, PH A59630 1

Silica-based SorbentsSorbent Phase 15 mg 30 mg Quantity

C18 A59603 A5960330 1

C18AR A59619 1

C2 A59601 1

C8 A59602 1

CN A59606 1

DAS A596DAS 1

DAU A596DAU 1

NH2 A59607 1

Phenyl A59610 1

SI A59608 1

Ion Exchange SorbentsSorbent Phase 15 mg Quantity

SAX A59605 1

SCX A59604 1

Mixed Mode SorbentsSorbent Phase 15 mg Quantity

MP1 A59611 1

MP3 A59620 1

PSA A59616 1

SCX/C8 A59623 1

SPEC Disc SPE


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SPEC DiscsFor a description of thse sorbents, see the Sorbent Selection Guide on pages 5-11.

SPEC DiscsSorbent Phase 47 mm 90 mm Quantity

C8 A74702 24 /Pk

C18ARA74819 20 /Pk A79019 12 /Pk

SPEC Disc Accessories

AccessoriesItem Name Part No. Quantity

SPEC Environmental Disc Manifold A712 1 Each

SPEC Environmental Disc Holder 47 mm A713 1 Each

SPEC 1 Liter Flask 40/35 mm A714 1 Each

SPEC Test Tube 25 mm x 250 mm A715 1 Each

SPEC® Well Plate AdaptersThe SPEC adapter allows the SPEC 96-well plate to fit properly into the collection plates used with Whatman, Waters, Porvair and similar vacuum manifolds. The adapter raises the SPEC 96-well plate 1.5 cm above the surface of the vacuum manifold to provide the clearance these systems require.

Item Name Part No. QuantitySPEC 96-well

Adapter A596AD 1

SPEC 96-well Adapter Gasket A596ADG 1

SPEC SPE CartridgesFor a description of these sorbents, see the Sorbent Selection Guide on pages 5-11.

SPEC 3 ml SPE Cartridges100/pack

Sorbent Phase 15 mg 30 mgC18 A5320320 A5320330

C18AR A5321920 A5321930 C18AR/MP1 A5322630 C18AR/MP3 A5322530

C2 A5320120 A5320130 C8 A5320220 A5320230 CN A5320620 A5320630 DAS A532DAS DAU A532DAU MP1 A5321120 A5321130 MP3 A5322020 A5322030 NH2 A5320720 A5320730 PSA A5321620 A5321630

Phenyl A5321020 A5321030 SAX A5320520 A5320530 SCX A5320420 A5320430

SCX/C8 A5322330 SI A5320820 A5320830

SPEC 10 ml SPE Cartridges100/pack

Sorbent Phase 35 mg 70 mgC18 A5020335

C18AR A5021935 C18AR/MP3 A5022570

C2 A5020135 C8 A5020235 CN A5020635

MP1 A5021135 A5021170 MP3 A5022035 NH2 A5020735 A5020770 PSA A5021635

Phenyl A5021035 SAX A5020535 SCX A5020435 SI A5020835

Empore Disk SPE


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Empore® Disk Cartridges

C18 HDHigh Density (12 µm particle size)

Disk Diameter(mm) 1 mL 3 mL 6 mL Quantity

(/Box)4 12144064 1007 12144065 5010 12144066 30

C18 SDStandard Density (55 µm particle size)

Disk Diameter(mm) 1 mL 3 mL 6 mL 40 mL Quantity

(/Box)4 12144001 1007 12144002 5010 12144003 3020 12144062 20



(/Box)4 12144007 1007 12144008 5010 12144009 30



(/Box)4 12144070 1007 12144071 5010 12144072 30



(/Box)4 12144004 1007 12144005 5010 12144006 30



(/Box)4 12144069 1007 12144068 5010 12144067 30

Empore Disk SPE


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MPC-Mixed Phase Cation HDHigh Density (12 µm particle size)

Disk Diameter(mm) 1 mL 3 mL 6 m Quantity

(/Box)4 12144040 1007 12144041 5010 12144042 30

MPC-Mixed Phase Cation SDStandard Density (55 µm particle size)


(/Box)4 12144073 1007 12144074 5010 12144075 30

SDB-RPSDisk Diameter

(mm) 1 mL 3 mL 6 mL Quantity(/Box)

4 12144055 1007 12144056 5010 12144057 30

SDB-XCDisk Diameter

(mm) 1 mL 3 mL 6 mL Quantity(/Box)

4 12144059 1007 12144060 5010 12144061 30

Empore Extraction Disks

Anion Exchange Extraction DisksDisk Diameter

(mm) Part No. Quantity(/Box)

47 12145012 2090 12145013 10

Chelating Extraction DisksDisk Diameter


47 12145029 2090 12145030 10

Oil & Grease Extraction DisksDisk Diameter


47 12145038 2090 12145039 10

SDB-RPS Extraction DisksDisk Diameter


47 12145026 2090 12145027 10

SDB-XC Extraction DisksDisk Diameter


47 12145010 2090 12145011 10

C8 Extraction DisksDisk Diameter


47 12145002 2090 12145034 10

C18 Extraction DisksDisk Diameter


47 12145004 2090 12145007 10

CarbonDisk Diameter

(mm) Part No. Quantity

47 12145040 20 /Box

Empore Disk SPE


20 Empore Disk SPE


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Empore Disk Plates

Deep Well Disk Plates2.5 mL capacity 96-well plates. Standard Density, 55 um particle size.

Sorbent Phase Part No. Quantity(Plate)

C18 SD 12143013 1

C2 SD 12143015 1

C8 SD 12143014 1

MPC SD 12143016 1

Standard Well Disk Plates1.2 mL capacity 96-well plates. Standard Density, 55 um particle size.

Sorbent Phase Part No. Quantity(Plate)

C18 SD 12143003 1

C2 SD 12143009 1

C8 SD 12143006 1

MPC SD 12143012 1

Empore Disk Plate Manifold & AccessoriesThe standard and deep well plates are compatible with the most commonly used automated liquid handling systems.

Empore Disk Plate Manifold

Item Name Part No. Quantity(Each)

Empore Disk 96-Well Plate Vacuum Manifold, Complete 12143100 1

Empore Disk Plate AccessoriesItem Name Part No. Quantity

96 Polypropylene Insert Collection Tube Rack 12143104 10 /Box

Deep Well, 96-Well Collection Plate 12143101 24 /Box

Empore Sealing Tape Pad #660 12143105 10 /PkReplacement Gasket for Empore Disk Plate Manifold 12143106 5 /Pk

U-Bottom 96-Well Collection Plate 12143103 100 /Box

V-Bottom 96-Well Collection Plate 12143102 60 /Box

Empore Filter Aid 400 - Glass BeadsEmpore Filter Aid 400 consists of 40 micron, nonporous, inert glass beads which act as a depth filtration material when placed on top of Empore Extraction Disks.

Empore Filter Aid 400 - Glass Beads


Filter Aid - Empore Glass Beads - 16 oz. 12146005 1

Empore® Disk ManifoldsThe one-, three- or six-station extraction vacuum manifold offers an efficient extraction apparatus. These manifolds allow multiple sample processing and are more stable and compact than a series of single glass filtration systems. Using the manifold, up to six extractions can be completed simultaneously, and a single operator can manage multiple manifolds. The powerful combination of the Empore disk and the six station manifold allows for up to 12 one-liter drinking water samples to be extracted per hour, a tremendous improvement over manual liquid/liquid methods.

The Empore Extraction Manifold accommodates a set of six 47 mm or three 90 mm glassware. Replacement parts may be ordered separately. A vacuum pump or supply is required.

Empore Disk Manifolds


Single Station 47 mm, Disk Vacuum Manifold, complete system 12146023 1

Single Station 90 mm, Disk Vacuum Manifold, complete system 12146022 1

Three Station 47 mm, Disk Vacuum Manifold, complete system 12146021 1

Six Station 90 mm, Disk Vacuum Manifold, complete system 12146002 1

Six Station 47 mm, Disk Vacuum Manifold, complete system 12146001 1

Empore Disk Manifold Replacement Components

Empore Disk Manifold Replacement ComponentsItem Name Part No. Quantity

300 mL Reservoir for Empore 47 mm Disk Manifold 12146015 1 Each



Clamp for 47 mm, Empore Disk Manifold 12146011 1 Each

Clamp for 90 mm, Empore Disk Manifold 12146012 1 Each

Empore Manifold Valve Assembly 12146007 1 Each

Empore Manifold Valve Stopcocks 12146006 6 /Box

Empore PTFE Sleeve 12146018 6 /BoxKEL-F Support Screen for Empore 47 mm Disk Manifold 12146009 6 /Box

KEL-F Support Screen for Empore 90 mm Disk Manifold 12146010 3 /Box

Replacement glass for Empore 47 mm Disk Manifold 12146003 1 Each

Replacement glass for Empore 90 mm Disk Manifold 12146004 1 Each

Support Base for Empore 47 mm Disk Manifold 12146017 1 Each

Support Base for Empore 90 mm Disk Manifold 12146014 1 Each

Teflon Coated Stainless Steel Support Screen for Empore 47 mm Disk Manifold 12146008 6 /Box

Bond Elut Cartridge SPE


22 Bond Elut Cartridge SPE


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Varian takes Bond Elut quality control to the next level. Optical scanners installed throughout our automated assembly process inspect each Bond Elut tube at multiple points. When defects or imperfections in the product are spotted, the tube is removed from the assembly line. The entire process is in compliance with the newest ISO 9001:2000 Quality System guidelines. The result is consistently reliable Bond Elut cartridges time and time again. With the attention we give to maintaining the highest possible quality standards, it’s no wonder Bond Elut has been the world’s leading SPE brand for over 20 years.

BOND ELUT®

• The original market-leading packed bed SPE cartridge• Unparalleled quality, service and support• 40 different bonded phases

Bond Elut is the original and still the most trusted name in solid phase extraction. Years of use by demanding scientists at top companies worldwide have thoroughly documented its many applications and proven its properties. To this day, you will find more literature references for Bond Elut than any other product in the industry.

Over 40 bonded phases are available in a variety of cartridge formats including straight wall, Laboratory Robot Compatible (LRC) and Bond Elut Junior. For information on available chemistries, refer to the Sorbent Selection Guide on pages 5-11.

The Varian Bond Elut Difference

• Ongoing Research and Development. Varian continually invests in research and technology to improve the production and quality of Bond Elut and other SPE products.

• World Class Manufacturing. Continual upgrades in materials and equipment help assure that the quality of Bond Elut remains ahead of the competition.

• Stringent Quality Control. Exacting ISO 9001: 2000 compliant manufacturing standards and inspections guarantee the consistent quality of Bond Elut

• Instant Availability. A large inventory of Bond Elut is maintained in locations worldwide to provide rapid response to customer orders.

• Technical Support. A team of expert scientists is available to consult on customer applications and help solve technical issues.

Bond Elut® AccuCATBond Elut AccuCAT cartridges are mixed-mode SPE columns consisting of a strong cation exchange and a strong anion exchange sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic, and neutral compounds from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from biofluids.

For assistance selecting the best sorbent for your application, please see the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and use our Applications Online Scanview database.

Bond Elut AccuCAT LRC CartridgesSorbent

Mass(mg)

Volume(mL) 40 µm Quantity

(/Box)

200 10 12282005 60600 10 12282001 60

Bond Elut AccuCAT Straight Barrel CartridgesSorbent

Mass(mg)


(/Box)

2003 12282003 606 12282004 30

400 6 12282006 30600 3 12282002 60





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Bond Elut® Alumina-A• Sorbent: alumina, Al2O3, acidic• pH: 4.5• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion exchange


Bond Elut Jr.

Sorbent Mass 25 µm Quantity(/Box)

500 mg 12162047B 100

1 g 12166043B 100

Bond Elut LRC CartridgesSorbent

Mass(mg)


(/Box)

100 10 12113020 50

500 10 12113046 50

Bond Elut Straight Barrel CartridgesSorbent

MassVolume

(mL) 25 µm Quantity(/Box)

50 mg 1 12102069 100

100 mg 1 12102021 100

500 mg 3 12102047 50

1 g 6 12256043 30

3 g 6 12256062 30

Bond Elut® Alumina-B• Sorbent: alumina, Al2O3, basic• pH: 10.0• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion exchange


Bond Elut Jr.


500 mg 12162048B 100

1 g 12166044B 100


Mass(mg)


(/Box)

100 10 12113021 50

500 10 12113047 50


MassVolume


50 mg 1 12102070 100

100 mg 1 12102022 100

500 mg 3 12102048 50

1 g 6 12256044 30





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Bond Elut® Alumina-N• Sorbent: alumina, Al2O3, neutral• pH: 7.5• Particle size: 25 µm• Primary retention mechanism: Lewis acid/base, polar, and ion exchange

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The smaller particle size ensures high extraction efficiency and small (50 mg) sorbent beds are therefore commonly used.

This sorbent is prepared with an electrically neutral surface. This favors the retention of electron-rich compounds like aromatic species and aliphatic amines. It also favors retention of compounds containing electronegative groups (for example, functional groups with oxygen, phosphorus and sulfur atoms). It maybe used in extracting either non-polar or polar species from aqueous or non-aqueous samples respectively.


Bond Elut Jr.


500 mg 12162049B 100

1 g 12166045B 100


Mass(mg)


(/Box)

100 10 12113022 50

500 10 12113048 50


MassVolume


50 mg 1 12102071 100

100 mg 1 12102023 100

500 mg 3 12102049 50

10 g 60 12256050 16

20 g 60 12256059 16

Bond Elut Cartridges with Stainless Steel FritsSorbent

Mass(mg)


(/Box)

100 1 12124023 100

500 3 12124046 50

Bond Elut Atrazine

Atrazine is a widely used herbicide in the United States that has been implicated in serious health problems, including tumor formation and endocrine problems. The biodegradation of atrazine and the harmful affects on the environment by some of its dealkylated metabolites, de-ethylatrazine and deisopropylatrazine, have placed these herbicide degradation products in the “watch list” of the National Pollutants Survey (NPA).

The Varian Bond Elut Atrazine cartridge is a specially bonded low-load, high-flow C18 phase. The controlled carbon content of the sorbent was designed to retain atrazine as well as its polar metabolites by a non-polar/polar retention mechanism. The bigger particle size of the sorbent and the diameter of the tube allow fast flow for large volume water samples. And, the large bed mass of the Bond Elut Atrazine phase has been optimized to provide enough capacity to ensure good recovery of atrazine and its polar metabolites.

With the use of the Bond Elut Atrazine extraction cartridge and optimized method for surface water (M2638) in combination with a Varian HPLC diode array-detection system, quantitative recoveries of atrazine and its dealkylated metabolites have been achieved, even at extremely low concentrations of 0.02 ppb. These concentration levels are more than two orders of magnitude below the tolerance level specified by the EPA (3 ppb) and those set by the most stringent European agencies (0.1 mg/L and 0.5 mg/L). The extraction and analysis methods are fast, reproducible and require minimal organic solvent consumption.


Mass(g)


(/Box)

3 20 12256111 20

BioBond Elut• Desalt synthetic oligonucleotides• No minimum molecular weight limit• Recoveries >98%

Choose BioBond Elut for fast, simple desalting of synthetic oligonucleotides from final deprotection reaction mixtures. With BioBond Elut there are no minimum molecular weight limits, so oligos less than 5,000 Daltons can be desalted with >98% recoveries. And, while typical desalting media can leave as much as 2% of the original salt in your sample, BioBond Elut provides 100% salt-free samples.


BioBond Elut Straight Barrel CartridgesSorbent

Mass(mg)


(/Box)

200 6 12102140 30500 6 12102141 30





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Bond Elut® C1• Base silica: 40 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: methyl• Endcapped: yes• Typical carbon loading: 4.1%• Primary retention mechanism:very weakly nonpolar• Typical sample types: plasma, urine, and aqueous samples

Bond Elut C1 sorbent

Due to the single carbon functional group, C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, because the sorbent is endcapped, masking the polar silanol activity, easier retention and elution of polar and multifunctional compounds can be achieved.



Mass(mg)


(/Box)

100 10 12113004 50300 10 12113053 50500 10 12113030 50


Mass(mg)


(/Box)

50 1 12102061 100

1001 12102004 1003 12102090 50

500 3 12102031 50


Mass(mg)


(/Box)

100 1 12124004 100500 3 12124028 50

Bond Elut® C2• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: ethyl• Endcapped: yes• Typical carbon loading: 5.6%• Primary retention mechanism: weakly nonpolar• Typical sample types: plasma, urine, and aqueous samples


C2 is a fairly polar sorbent because of the short chain length of the functional group, which exposes the silica surface. C2 is often used as a replacement for C18 and C8 when molecules are retained too strongly by the latter. C2’s polarity is slightly lower than a CN for polar interactions. It is popular for the extraction of drugs from plasma and serum since, if correct wash and elution conditions are selected, flat baselines can be achieved.



Mass(mg)

Volume(mL) 40 µm 120 µm Quantity

(/Box)

100 10 12113003 14113003 50200 10 12113026 14113026 50500 10 12113029 14113029 50


MassVolume

(mL) 40 µm 120 µm Quantity(/Box)

50 mg1 12102060 14102060 1003 12105029 50

100 mg1 12102003 14102003 1003 12102117 50

200 mg 3 12102027 14102027 50

500 mg3 12102030 506 12102115 30

1 g 6 12256003 14256003 302 g 12 12256056 205 g 20 12256141 2010 g 60 12256057 16


Mass(mg)


(/Box)

100 1 12124003 14124003 100500 3 12124027 14124027 50





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Bond Elut® C8• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: octyl• Endcapped: yes• Typical carbon loading: 12.2%• Primary retention mechanism: moderately nonpolar• Typical sample types: water, aqueous biological samples


C8 is very similar in property to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain. C8 is a good replacement for C18 when analytes are too strongly retained on C18 for effective elution. The potential of C8 for polar interactions with analytes is somewhat higher than for C18 because the shorter hydrocarbon chain does not mask the silica surface as effectively, but polar interactions are still not a significant property of C8. The C8 sorbent has been successfully utilized in the simultaneous extraction of fat- and water-soluble vitamins from human serum.


Bond Elut Jr.Sorbent Mass

(mg) 40 µm Quantity(/Box)

500 12162029B 100


Mass(mg)


(/Box)

100 10 12113002 14113002 50200 10 12113025 14113025 50500 10 12113028 14113028 50


MassVolume


50 mg1 12102059 14102059 1003 12105028 50

100 mg1 12102002 14102002 1003 12102100 50

200 mg 3 12102026 14102026 50

500 mg3 12102029 14102029 506 12102053 14102053 30

1 g 6 12256002 14256002 302 g 12 14256016 205 g 20 12256024 14256024 2010 g 60 12256032 14256032 16


Mass(mg)


(/Box)

100 1 12124002 14124002 100

5003 12124026 14124026 506 124002 30

Bond Elut® C18• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60

Å mean pore size• Bonded functional group: trifunctional octadecyl• Endcapped: yes• Typical carbon loading: 17.4%• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids


C18 is the most hydrophobic silica-based sorbent available. It is the most popular SPE sorbent because of its extreme retentive nature for nonpolar compounds. C18 is generally regarded as the least selective silica based sorbent, since it retains most organic analytes from aqueous matrices, often a benefit when the compounds of interest vary widely in structure. The potential for polar interactions between analytes and sorbent is less significant with C18 than with any other sorbent because of the predominant effect of the long hydrocarbon chain. When analyzing small to intermediate molecules, C18 can also be utilized for desalting aqueous matrices prior to ion exchange because salts pass through it unretained.


Bond Elut Jr.


500 mg 12162028B 1001 g 12166001B 100


Mass(mg)


(/Box)

100 10 12113001 14113001 50200 10 12113024 14113024 50500 10 12113027 14113027 50


MassVolume


50 mg1 12102058 14102058 1003 12105027 50

100 mg1 12102001 14102001 1003 12102099 50

200 mg1 12102096 1003 12102025 14102025 50

500 mg3 12102028 14102028 506 12102052 14102052 30

1 g3 12102118 506 12256001 14256001 3060 12256060 16

1.5 g 3 12102149 502 g 12 14256015 205 g 20 14256023 2010 g 60 14256031 16

(continued)





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Bond Elut C18 Cartridges with Stainless Steel FritsSorbent

MassVolume


100 mg 1 12124001 100

500 mg3 12124025 14124025 506 124004 30

1 g 6 12124110 30

Bond Elut® C18 EWP• Base silica: 40 µm irregularly-shaped, acid-washed silica, 500 Å mean pore size• Bonded functional group: trifunctional octadecyl• Endcapped: yes• Typical carbon loading: 12.2%• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids


The C18 EWP sorbent is based upon standard particle size silica but with 500 Å pores to allow more efficient extraction of macromolecules typical of most bimolecular separations. The larger pore size of the sorbent enables large molecules (> 15,000 MW), which are typically excluded from standard porosity phases, to permeate into the pores and interact with the bonded silica surface. With C18 EWP, successful separation of complex mixtures of proteins, peptides or nucleotides can be achieved. Also, C18 EWP is a good sorbent choice for desalting matrices with large molecular weight com-pounds of interest prior to LC/MS or LC/MS/MS, capillary electrophoresis or any other analytical technique that is adversely affected by the presence of salts in the sample. (Note: see BioBond Elut section on optimized bed mass and application for desalting oligonucleotides.)



Mass(mg)


(/Box)

50 10 12113068 50

100 10 12113069 50

200 10 12113070 50

500 10 12113071 50


MassVolume


50 mg 1 12102136 100

100 mg 1 12102137 100

500 mg 3 12102139 50

200 mg 3 12102138 50

1 g 6 12256130 30

Bond Elut® C18 INT• Base silica: 40 µm irregularly-shaped, acid-washed silica, 500 Å mean pore size• Bonded functional group: trifunctional octadecyl• Endcapped: yes• Typical carbon loading: 12.2%• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids


C18 INT is very similar in properties to the standard Varian C18 sorbent but with a slightly lower carbon loading. This intermediate load bonded C18 is also thoroughly end capped ensuring rapid release of basic drugs. The carbon content has been optimized to differentiate between metabolites of species like prostaglandins. In addition, the narrow pore size helps exclude large molecules such as proteins.



Mass(mg)


(/Box)

50 10 12113120 50

100 10 12113121 50

200 10 12113122 50

500 10 12113123 50


MassVolume


50 mg 1 12102120 100

100 mg 1 12102121 100

200 mg 3 12102122 50

500 mg 3 12102123 50

1 g 6 12256120 30





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Bond Elut® C18 LO• Base silica: 40 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: trifunctional octadecyl• Endcapped: yes• Typical carbon loading: 4.7%• Primary retention mechanism: strongly nonpolar• Typical sample types: water, aqueous biological fluids


C18 LO, like C18 and C18 INT, is a highly retentive nonpolar silica-based sorbent due to the long hydrocarbon chain. However, the low carbon loading of C18 LO gives unique selectivities over other octadecyl sorbents. The percent carbon loading has been optimized for polar analyte extraction from environmental matrices. Quantitative recoveries of Atrazine metabolites have been successfully achieved with C18 LO sorbents. (Note: see Bond Elut Atrazine section for optimized bed mass and application information.)



Mass(mg)


(/Box)

50 10 12113110 50

100 10 12113111 50

200 10 12113112 50

500 10 12113113 50


MassVolume


50 mg 1 12102110 100

100 mg 1 12102111 100

200 mg 3 12102112 50

500 mg 3 12102113 50

1 g 6 12256110 30

Bond Elut® C18 OH• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica,

150 Å mean pore size• Bonded functional group: octadecyl• Endcapped: no• Typical carbon loading: 14.9%• Primary retention mechanism: moderately nonpolar, plus significant

polar secondary interactions• Typical sample types: biological matrices, aqueous samples


C18 OH is a nonendcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has controlled silanol activity under tight QC tolerances for best batch-to-batch reproducibility. The silanol activity permits fractionation of metabolites and enhances retention of basic compounds compared with endcapped C18. In addition, the 150 Å intermediate pore size is good for intermediate molecular weight compounds that may not be retained on a standard 60 Å material.


Bond Elut Jr.Sorbent

Mass(mg)

40 µm Quantity(/Box)

500 12162046B 100


Mass(mg)


(/Box)

100 10 12113019 14113019 50

500 10 12113045 14113045 50


MassVolume


50 mg 1 12102065 14102065 100

100 mg 1 12102020 14102020 100

500 mg 3 12102046 14102046 50

1 g 6 12256040 14256040 30


Mass(mg)


(/Box)

100 1 12124020 14124020 100

500 3 12124043 14124043 50





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Bond Elut® CBA• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: carboxylic acid• Sorbent pKa: 4.8• Mean capacity: 0.35 meq/g• Endcapped: yes• Typical percent carbon loading: 7.4%• Primary retention mechanism: weak cation exchange• Typical sample types: water, biological fluids

Bond Elut CBA carboxylic acid sorbent

CBA is a medium polarity sorbent based on carboxy propyl functionalized silica, exhibiting either polar or nonpolar interactions depending on the matrix or solvent environment. Its prime characteristic is its weak cation exchange properties. Because of its pKa value of 4.8, CBA does not require extreme basic conditions for elution that are required by other cation exchange sorbents. Above a pH of 4.8, the majority of functional groups carry a negative charge that can be used for retaining cationic compounds. Dropping the pH to 2.8 effectively neutralizes CBA, permitting the retained analytes to elute from the sorbent. For this reason, CBA is often the best choice for cation exchange, especially when dealing with very strong cations (i.e. high pKa cations). In addition, CBA shows a wider range of selectivity towards counter-ions than other cation exchange phases, enabling more options for elution.



Mass(mg)


(/Box)

100 10 12113011 14113011 50

500 10 12113037 14113037 50


MassVolume


50 mg 1 12102073 100

100 mg1 12102011 14102011 100

3 12102097 50

200 mg 3 12102124 50

500 mg 3 12102038 14102038 50

1 g 6 12256009 14256009 30

2 g 12 12256058 20


Mass(mg)


(/Box)

100 1 12124011 14124011 100

500 3 12124035 14124035 50

Bond Elut® Cellulose

The Varian Bond Elut Cellulose column utilizes a pure microgranular cellulose powder that is packed between two 20 µm polyethylene frits in an ultraclean 3 mL polypropylene extraction column. The unique rod-like shape and controlled particle size of this variety of Cellulose ensure that it is free-flowing when dry. In addition, the material is manufactured from high purity cotton with a very high a-cellulose content that ensures optimum column packing properties. Thus, high density, more uniform, smooth-packed column beds are achieved. In addition, the cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and Cu content less than 5 ppm). The combination of surface area and polymeric structure results in a sorbent with excellent capacity.

The cellulose media contains numerous hydroxyl groups. Because of its polar nature, it is able to accept high loading of many polar substances from aqueous and organic phases. The free hydroxyl groups’ binding properties are complemented by the presence of very small quantities of residual aldehydes, ketones and carboxyl groups. These weak groups add reducing and ion-exchange properties.

This microgranular cellulose support has been utilized for large scale bioprocessing as well as a prefilter for suspended matter (i.e. cell debris and suspended fats), as a prefractionation step prior to reverse phase HPLC, for separation of biomolecules, and as an affinity chromatography matrix.



Mass(mg)

Volume(mL) Part No. Quantity

(/Box)

300 3 12102095 500





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Bond Elut Certify®

• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: Mixed-mode sorbent bed: octyl (C8) and

benzenesulfonic acid (SCX)• Endcapped: No• Typical percent carbon loading: 9.0%• Primary retention mechanism: Bimodal, nonpolar and strong cation

exchange• Typical sample types: Urine, biological fluids


Mass(mg)


(/Box)

130 10 12113050 14113050 50

200 10 12113054 14113054 50

300 10 12113052 14113052 50


MassVolume


50 mg 3 12105030 50

130 mg3 12102051 14102051 50

6 12256146 30

200 mg3 12102145 50

6 12256145 30

300 mg3 12102081 50

6 12102082 30

500 mg 6 12102093 14102093 30

1 g 6 12102085 14102085 30

Bond Elut Certify® II• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60

Å mean pore size• Bonded functional group: mixed-mode sorbent bed: octyl and

quaternary amine (SAX)• Endcapped: no• Typical percent carbon loading: 8.6%• Primary retention mechanism: bimodal, nonpolar and strong anion

exchange• Typical sample types: urine, biological fluids

Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and strong anion exchange (SAX) sorbent. It has been optimized for extraction of acidic drugs such as 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid, salicylic acid, ibuprofen, acetaminophen, and other compounds possessing both nonpolar and anionic character.



Mass(mg)


(/Box)

100 10 12113063 50

200 10 12113051 14113051 50


MassVolume


50 mg 3 12105031 50

200 mg 3 12102080 14102080 50

500 mg6 12102084 30

6 14102084 30

1 g 6 12102088 14102088 30





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Bond Elut® CH (cyclohexyl)• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: cyclohexyl• Endcapped: yes• Typical carbon loading: 9.6%• Primary retention mechanism: moderately nonpolar• Typical sample types: water, aqueous samples

Bond Elut CH cyclohexyl sorbent

Cyclohexyl is a medium polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a nonpolar sorbent, CH has the approximate polarity of a C2 sorbent. The polar subsurface is an important factor among CH properties, as indicated by the ability of CH to retain polar analytes such as phenol from aqueous matrices. Because of its unique selectivity, CH is often a good choice when other nonpolar sorbents such as C18, C8, C2, and PH do not provide the desired selectivity.



Mass(mg)


(/Box)

100 1 12113006 14113006 50

500 3 12113032 14113032 50


MassVolume


50 mg 1 12102063 14102063 100

100 mg 1 12102006 14102006 100

500 mg 3 12102033 14102033 50

1 g 6 12256005 14256005 30

2 g 12 12256039 14256039 20


Mass(mg)


(/Box)

100 1 12124006 14124006 100

500 3 12124030 14124030 50

Bond Elut® CN-E• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: cyanopropyl• Endcapped: yes• Typical carbon loading: 8.1%• Primary retention mechanism: moderately nonpolar (aqueous matrix)

or polar (nonpolar organic matrix)• Typical sample types: aqueous (nonpolar), organic (polar)

Bond Elut CN-E cyanopropyl sorbent

A medium polarity sorbent with many uses, CN-E is ideal for applications in which extremely nonpolar compounds would irreversibly retain on more nonpolar sorbents, such as C18 or C8. This endcapped version of the cyano sorbent is best utilized when extracting analytes from an aqueous matrix.



Mass(mg)


(/Box)

100 10 12113007 14113007 50

500 10 12113033 14113033 50


MassVolume


50 mg 1 12102064 14102064 100

100 mg 1 12102007 14102007 100

500 mg 3 12102034 14102034 50

5 g 20 12256025 20


Mass(mg)


(/Box)

100 1 12124007 14124007 100

500 3 12124031 14124031 50





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Bond Elut® CN-U• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: cyanopropyl• Endcapped: no• Typical percent carbon loading: 7.8%• Primary retention mechanism: polar (nonpolar organic matrix) or weak

nonpolar (aqueous matrix)• Typical sample types: nonpolar solvents, organic extracts of aqueous

sample

Bond Elut CN-U cyanopropyl sorbent

Unendcapped CN-U is a medium polarity sorbent. It is less retentive than Si or 2OH for very polar analytes that might be retained irreversibly on these more polar sorbents. This Unendcapped cyano phase is ideally suited for the extraction of polar compounds from a nonpolar matrix such as hexane or oils.



Mass(mg)


(/Box)

100 10 12113008 14113008 50

500 10 12113034 14113034 50


MassVolume


50 mg 1 12102066 14102066 100

100 mg 1 12102008 14102008 100

200 mg 3 12102135 50

500 mg 3 12102035 14102035 50

1 g 6 12256006 14256006 30

2 g 12 12256017 14256017 20

5 g 20 14256025 20

10 g 60 12256033 14256033 16


Mass(mg)


(/Box)

100 1 12124008 14124008 100

500 3 12124032 14124032 50

Bond Elut® DEA• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: diethylaminopropyl• Sorbent pKa: 10.7• Mean capacity: 1.0 meq/g• Endcapped: no• Typical percent carbon loading: 8.5%• Primary retention mechanism: weak anion exchange (aqueous matrix)

or polar (nonpolar organic matrix)• Typical sample types: water, biological fluids, nonpolar extracts

Bond Elut DEA diethylaminopropyl sorbent

Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties, as does Bond Elut PSA. DEA has slightly lower capacity as an anion exchange sorbent (1.0 meq/g) than NH2, and a more nonpolar character due to the additional carbon chain on the functional group. The carbon chains make DEA a medium polarity sorbent, even with the presence of the main functionality. DEA is somewhat more polar than C8, but less polar than C2 or CN.



Mass(mg)


(/Box)

100 10 12113016 14113016 50

500 10 12113042 14113042 50


Mass(mg)


(/Box)

50 1 12102078 14102078 100

100 1 12102016 14102016 100

500 3 12102043 14102043 50


Mass(mg)


(/Box)

100 1 12124016 14124016 100

500 3 12124040 14124040 50





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Bond Elut® ENV• Sorbent: polymeric wide-pore styrene-divinylbenzene (SDVB) polymer• Applications for explosive residues• Excellent for polar biomolecules• Used for sulfated or glucuronide-bound steroids• High flow-through: 1 liter in less than 20 minutes• Efficient elutions

Bond Elut ENV, a high purity styrene-divinylbenzene (SDVB) polymer, has been optimized for the extraction of polar organic residues, such as herbicide metabolites and explosives, from large volume water samples. Bond Elut ENV features a 125 µm highly cross-linked spherical polymer that can process one liter water samples in a fraction of the time required by other sample preparation methods. The absence of secondary interactions on the polymer surface makes it easier to elute a wide range of molecular species with just one elution solvent. Although primarily used for environmental applications, Bond Elut ENV can also be used for clinical applications including the extraction of highly polar metabolites, such as glucuronide-bound molecules from human fluids.



MassVolume


25 mg 1 12105011 100

50 mg 1 12105012 100

60 mg 3 12105017 50

100 mg1 12105013 100

3 12105014 50

200 mg3 12105015 50

6 12255014 30

500 mg3 12105016 50

6 12255011 30

1 g 6 12255012 30

Bond Elut® Diol (2OH)• Base silica: 40 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: diol• Endcapped: no• Typical percent carbon loading: 6.8%• Primary retention mechanism: polar• Typical sample types: nonpolar organic extracts, oils, and lipids

Bond Elut diol sorbent


Bond Elut 2OH LRC CartridgesSorbent

Mass(mg)


(/Box)

100 10 12113009 50

500 10 12113035 50

Bond Elut 2OH Straight Barrel CartridgesSorbent

MassVolume


50 mg 1 12102067 100

100 mg 1 12102009 100

500 mg 3 12102036 50

1 g 6 12256007 30

EnvirElut® Pesticide, Herbicide & PAH CartridgesThe extreme purity and high capacity of the EnvirElut sorbents make them especially suitable for the extraction of a wide range of compounds from aqueous matrices. Every lot of EnvirElut is rigorously tested for retention of environmental pollutants from water samples before it is approved for sale. The EnvirElut sorbent is then packed into the straight barrel Bond Elut cartridge configuration most suitable for each specific environmental analysis. EnvirElut Herbicides, PAH, Pesticides, and Phenols use conventional SPE cartridges that are processed using conventional Vac Elut vacuum manifolds, and they are not compatible with the EnvirElut 1664 manifold system.

Bond Elut Straight Barrel Cartridges

Sorbent Phase Volume(mL)

Sorbent Mass Part No. Quantity

(/Box)HERBICIDE 3 500 mg 12272006 50

PAH 6 1 g 12272005 30

PESTICIDE 6 500 mg 12272004 30

PHENOLS 20 5 g 12272002 20





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Bond Elut® Florisil• Sorbent: Florisil - MgO3Si• Particle size: 200 µm• Primary retention mechanism: polar• Typical sample types: environmental, organic extracts

Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for the isolation of polar compounds from nonpolar matrices. The larger particle size of the Florisil material enables fast flow of large volume samples and thus is a potential alternative to silica when using viscous samples. Florisil is utilized for the separation of chlorinated pesticides and is used in numerous AOAC- and EPA-regulated methods.



Mass 200 µm Quantity(/Box)

500 mg 12162050B 100

1 g 12166014B 100


Mass(mg)


(/Box)

100 10 12113023 50

500 10 12113049 50


MassVolume


50 mg 1 12102072 100

100 mg 1 12102024 100

500 mg 3 12102050 50

1 g

3 12102109 50

6 12256014 30

20 12256047 20

2 g12 12256022 20

20 12256046 20

5 g 20 12256030 20

10 g 60 12256038 16


MassVolume


100 mg 1 12124024 100

500 mg 3 12124047 50

1 g3 12126013 50

6 124007 30

Bond Elut® LMS• Sorbent: ultraclean, highly cross-linked styrene-divinylbenzene (SDVB)

polymer• Optimized 75 µm particle size for easy throughput of biological

samples such as urine, plasma, or serum• High, controlled surface area for consistent extraction efficiency• High capacity allows larger sample loads or a smaller sorbent bed,

resulting in better analyte concentration• Removes proteins efficiently• Worry-free use for SPE and GC/MS and LC/MS applications

Varian’s Bond Elut LMS polymeric sorbent lets you elute samples without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase compatible solvent mixtures of low ionic strength. These characteristics are crucial in ensuring compatibility with LC/MS or other sensitive analytical techniques. Featuring optimized particle size and a large surface area for extraction efficiency, Bond Elut LMS provides interference-free extractions. Producing excellent recovery and reproducibility, the LMS sorbent will ensure worry-free use and reduce costly downtime.



Mass(mg)


(/Box)

50 10 12115022 50

100 10 12115023 50


MassVolume


25 mg 1 12105021 100

50 mg 1 12105022 100

100 mg1 12105023 100

3 12105024 50

200 mg 3 12105025 50

500 mg3 12105026 50

6 12255021 30

1 g 6 12255022 30





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Bond Elut® NH2• Base silica: 40 µm or 120 µm irregularly shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: aminopropyl• Sorbent pKa: 9.8• Mean capacity: 1.10 meq/g• Endcapped: no• Typical carbon loading: 6.7%• Primary retention mechanism: weak anion exchange (aqueous matrix)

or polar (nonpolar organic matrix)

Bond Elut NH2 aminopropyl sorbent

NH2, a very polar sorbent, can utilize both hydrogen bonding and anion exchange. Since the pKa of the NH2 sorbent is 9.8, at any pH below 9.8, the majority of the functional groups are positively charged. NH2 is a weaker anion exchanger than sorbents like SAX (a quaternary amine sorbent that is always charged) and is therefore a better sorbent choice for retention of very strong anions, such as sulfonic acids, which may be retained irrevers-ibly on SAX. Because an ethyl group supports the NH2 functionality, it can be used for nonpolar isolations from polar samples, but its strong polarity is its primary characteristic. Like 2OH and SI, NH2 is excellent for separa-tion of structural isomers.




500 mg 12162041B 1001 g 12166012B 100


Mass(mg)


(/Box)

100 10 12113014 14113014 50200 10 12113067 50500 10 12113040 14113040 50


MassVolume


50 mg 1 12102076 14102076 100100 mg 1 12102014 14102014 100

200 mg3 12102089 506 12102106 30

300 mg 3 12102108 50

500 mg3 12102041 14102041 506 12256045 30

1 g3 12102107 506 12256012 14256012 30

2 g 12 14256020 205 g 20 14256028 2010 g 60 14256036 16


Mass(mg)


(/Box)

100 1 12124014 14124014 100500 3 12124038 14124038 50

Bond Elut® PBA• Base silica: 40 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: phenylboronic acid• Endcapped: no• Typical percent carbon loading: 7.9%• Primary retention mechanism: covalent bond with analyte possessing

vicinal diol groups• Typical sample types: aqueous, biological fluids

Bond Elut PBA phenylboronic acid sorbent

PBA is a unique sorbent consisting of a phenylboronic acid covalently linked to a silica gel surface. Because covalent retention involves an interaction with 10-100 times greater energy than other extraction mechanisms, compounds are more tightly bound to the sorbent surface resulting in a more complete cleanup. The boronate group has a high specificity for cis-diol-containing compounds like catechols, nucleic acids, low molecular weight proteins and carbohydrates. PBA has proven to be especially effective in the isolation of catecholamines from biological

fluids, and for the separation of RNA from DNA. Also, it may be applied to cis-amino alcohols such as propanol. Retention is usually strongest when the analytes’ functional groups are coplanar.



Mass(mg)


(/Box)

100 10 12113018 50


Mass(mg)


(/Box)

1001 12102018 201 12102019 100

500 6 12102105 30





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Bond Elut® PCB

Using the Bond Elut PCB cartridge with an optimized method (M2647), quantitative recoveries of a wide range of PCB compounds have been obtained using a simple procedure that uses only one solvent. When the Bond Elut PCB SPE column is used in combination with the CP-Select 28/31 capillary column (Part Number CP7479), a wide range of PCB compounds, including PCB 28 and 31, can be isolated and resolved in half the time of other SPE and GC procedures.



Mass(g)


(/Box)

1 3 12105032 50

Bond Elut® PH• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: phenyl• Endcapped: yes• Typical carbon loading: 10.7%• Primary retention mechanism: moderately nonpolar• Typical sample types: water, biological fluids

Bond Elut PH phenyl sorbent

Our Bond Elut PH phenyl sorbent is most commonly employed for nonpolar extractions, with a similar polarity to a C8 sorbent. Like cyclohexyl, PH exhibits a slightly different selectivity from other nonpolar sorbents. This added selectivity results from the electron density of the aromatic ring. Retention of planar, conjugated organic molecules is enhanced compared to the aliphatic bonded phases.


Bond Elut PH LRC CartridgesSorbent

Mass(mg)


(/Box)

100 10 12113005 14113005 50

500 10 12113031 14113031 50

Bond Elut PH Straight Barrel CartridgesSorbent

MassVolume


50 mg 1 12102062 14102062 100

100 mg 1 12102005 14102005 100

500 mg 3 12102032 14102032 50

1 g 6 12256004 14256004 30

Bond Elut PH Cartridges with Stainless Steel FritsSorbent

Mass(mg)


(/Box)

100 1 12124005 14124005 100

500 3 12124029 14124029 50

Bond Elut® PPL• Sorbent: unique functionalized styrene-divinylbenzene (SDVB) polymer• Perfect for phenols in waste water• High surface area for extreme retention of polar analytes• High flow properties, even for dirty water

Bond Elut PPL, a styrene-divinylbenzene (SDVB) polymer, has been optimized for the extraction of highly polar species from large volume water samples. Featuring a high purity spherical polymer which has a proprietary, derivitized, nonpolar surface, Bond Elut PPL retains even the most polar classes of compounds, such as phenol, and achieves clean extracts, high recovery levels, and fast extraction speeds. Its extreme hydrophobicity and surface area of 600 m2/g ensures reproducibility, quantitative retention, and the highest possible recoveries.



MassVolume


25 mg 1 12105001 10050 mg 1 12105002 100

100 mg1 12105003 1003 12105004 50

200 mg 3 12105005 50

500 mg3 12105006 506 12255001 30

1 g3 12102148 506 12255002 30





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Bond Elut® PRS• Base silica: 40 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: propylsulfonic acid• Sorbent pKa: <1.0• Mean capacity: 0.18 meq/g• Endcapped: no• Typical percent carbon loading: 1.7%• Primary retention mechanism: strong cation exchange• Typical sample types: water, biological samples

Bond Elut PRS propylsulfonic acid sorbent

A strong cation exchange sorbent that is also very polar, PRS does not exhibit any appreciable degree of nonpolar interactions. In nonpolar solvents, PRS is capable of polar and hydrogen bonding interactions. As the pKa of PRS is low, cationic compounds must usually be eluted either by a high ionic strength eluant, or by neutralizing the charge on the cationic isolate. Therefore, PRS is most effective for weaker cations such as pyridinium compounds. The rela-tive lack of nonpolar secondary interactions, combined with its low capacity, gives PRS a unique set of selectivity properties.



Mass(mg)


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100 10 12113012 50

500 10 12113038 50


MassVolume


50 mg 1 12102074 100

100 mg 1 12102012 100

200 mg 3 12102094 50

500 mg 3 12102039 50

1 g 6 12256010 30


Mass(mg)


(/Box)

100 1 12124012 100

500 3 12124036 50

Bond Elut® PSA• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: ethylenediamine-N-propyl• Sorbent pKa: 10.1 and 10.9• Mean capacity: 1.40 meq/g• Endcapped: no• Typical percent carbon loading: 7.5%• Primary retention mechanism: weak anion exchange (aqueous matrix),

or polar (nonpolar organic matrix), or chelation• Typical sample types: water, biological fluids, and organic extracts

Bond Elut PSA ethylenediamine-N-propyl sorbent

PSA is similar to NH2. An anion exchange sorbent, PSA has two amine groups that offer higher ionic capacity than NH2 (1.4 meq/g vs. NH2’s 1.1 meq/g), and the pKas of its primary and secondary amine groups are higher (10.1 and 10.9, respectively). PSA is therefore a stronger anion exchanger than NH2. The PSA functional group is a very good bidentate ligand, making PSA an excellent sorbent for chelation. Its higher carbon content makes it a more nonpolar sorbent than NH2 and thus a better choice for very polar compounds that retain too strongly on NH2 sorbent.




500 mg 12162042B 100

1 g 12166050B 100


Mass(mg)


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100 10 12113015 14113015 50

500 10 12113041 14113041 50


MassVolume


50 mg 1 12102077 14102077 100

100 mg 1 12102015 14102015 100

500 mg 3 12102042 14102042 50

1 g 6 12256140 30

2 g 12 12256055 20

3 g 6 12105044 30

10 g 60 12256041 16


Mass(mg)


(/Box)

100 1 12124015 14124015 100500 3 12124039 14124039 50





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Bond Elut® SAX• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: trimethylaminopropyl• Sorbent pKa: quaternary amine, always charged• Mean capacity: 0.85 meq/g• Endcapped: no• Typical percent carbon loading: 7.5%• Primary retention mechanism: strong anion exchange• Typical sample types: aqueous, biological samples

Bond Elut SAX trimethylaminopropyl sorbent

SAX is the strongest anion exchange sorbent available. Because its functional group is a quaternary amine, the sorbent is always charged. SAX offers minimal nonpolar interactions because any effect of the carbon atoms in the functional group is masked by the amine. Because SAX is such a strong anion exchanger, it is a good sorbent choice for retention of weaker anions such as carboxylic acids that may not retain strongly enough on weaker anion exchange phases. It is supplied in the chloride counter-ion form. The user may modify its selectivity by conditioning it with appropriate buffers, as described in The Handbook of Sorbent Extraction Technology (P/N 12245005).


Bond Elut Jr.


500 mg 12162044B 1001 g 12166013B 100


Mass(mg)


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100 10 12113017 14113017 50500 10 12113043 14113043 50


MassVolume


50 mg 1 12102079 14102079 100

100 mg1 12102017 14102017 1003 12102125 50

500 mg3 12102044 14102044 506 12102144 30

1 g3 12102087 506 12256013 14256013 30

1.5 g 3 12102103 50

2 g6 12256051 3012 12256021 14256021 20

3 g 6 12256052 305 g 20 12256029 14256029 2010 g 60 12256037 14256037 16


Mass(mg)


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100 1 12124017 14124017 100500 3 12124041 14124041 50

Bond Elut® SCX• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60 Å mean pore size• Bonded functional group: benzenesulfonic acid• Sorbent pKa: <1.0• Mean capacity: 0.79 meq/g• Endcapped: no• Typical percent carbon loading: 10.9%• Primary retention mechanism: strong cation exchange• Typical sample types: aqueous, biological fluids

Bond Elut SCX benzenesulfonic acid sorbent

SCX is a strong cation exchanger with a very low pKa, similar to PRS. However the SCX sorbent has a higher potential for nonpolar interactions than PRS, due to the presence of the benzene ring on its surface. This nonpolar char-acter of SCX should be considered when the sorbent is used for ion exchange from aqueous solvent systems. This dual nature is useful with compounds that exhibit both cationic and nonpolar character. The overall result of the dual character is superior clean up using only a single sorbent.


Bond Elut Jr.


500 mg 12162040B 1001 g 12166011B 100


Mass(mg)


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100 10 12113013 14113013 50500 10 12113039 14113039 50


MassVolume


50 mg 1 12102075 14102075 100

100 mg1 12102013 14102013 1003 12102098 14102040 50

500 mg 3 12102040 501 g 6 12256011 14256011 30

1.5 g 3 12102104 50

2 g6 12256053 3012 14256019 20

3 g 6 12256054 305 g 20 14256027 2010 g 60 14256035 16


Mass(mg)


(/Box)

100 1 12124013 14124013 100500 3 12124037 14124037 50





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Bond Elut® SI• Base silica: 40 µm or 120 µm irregularly-shaped silica, 60 Å mean pore size• Bonded functional group: native silica, unfunctionalized• Endcapped: no• Typical percent carbon loading: 0%• Primary retention mechanism: strongly polar• Typical sample types: nonpolar organic solvents, oils, and lipids

Bond Elut SI silica sorbent

Silica is generally regarded as the most polar sorbent available. Unbonded, activated silica is also fairly acidic. SI is one of the best sorbents available for selectively separating compounds of very similar structure. Applying the analytes in a nonpolar solvent, then increasing the solvent polarity by adding increasing concentrations of polar modifiers, such as THF or ethyl-acetate, at successive steps can accomplish this separation.




500 mg 12162037B 100

1 g 12166008B 100


Mass(mg)


(/Box)

100 10 12113010 14113010 50

500 10 12113036 14113036 50


MassVolume


50 mg 1 12102068 14102068 100

100 mg 1 12102010 14102010 100

200 mg 20 12102147 20

500 mg 3 12102037 14102037 50

1 g 6 12256008 14256008 30

1.5 g 3 12102119 50

2 g 12 14256018 20

5 g 20 14256026 20

10 g 60 14256034 16


Mass(mg)


(/Box)

100 1 12124010 14124010 100

500 3 14124034 50

Bond Elut® TCA• Fast, low-cost tricyclic antidepressants analysis• Pretested to give average recoveries >90%

Each lot of Bond Elut TCA material is manufactured to exacting standards and tested specifically for drug recovery. Both a straight barrel and a LRC Bond Elut configuration are available, packed with 100 mg of the Bond Elut TCA sorbent.



Mass(mg)


(/Box)

100 10 12113062 50


Mass(mg)


(/Box)

100 3 12102146 50

Bond Elut® Layered Phase CartridgesThree PSA and SAX layered columns are available which can be utilized in the USFDA Luke method for multiresidue pesticide analysis. Two straight-walled Bond Elut versions are available containing 250 mg of each phase and 500 mg of each phase. In addition, the newly redesigned Bond Elut Jr. cartridge enables the PSA/ SAX combination, 500 mg per phase, to be packed into a single cartridge.


Bond Elut Straight Barrel Cartridges

Item Name Part No. Quantity(/Box)

AL-N III/SI-HF, 1g of each phase in a 6 mL Bond Elut cartridge 12256063 30

FL/C18, 2g of each phase in a 20 mL Bond Elut cartridge 12256142 20

SAX/PSA, 250 mg of each phase in a 3 mL Bond Elut cartridge 12102116 30

SAX/PSA, 500 mg of each phase in a 6 mL Bond Elut cartridge 12256061 30

Si/CN-E, 500 mg of each phase in a 6 mL Bond Elut cartridge 12256066 30

Bond Elut Jr.

Item Name Part No. Quantity(/Pk)

SAX/PSA, 500 mg of each phase in a Bond Elut Jr. cartridge 12166052B 100





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ASPEC Capped SPE Bond Elut® Cartridges• ASPEC-compatible Bond Elut cartridges• Eleven popular Bond Elut sorbents• Three cartridge sizes• Convenient and cost-effective

Varian’s standard 1, 3 and 6 mL Bond Elut cartridges are fitted with special caps that produce a positive pressure seal with the needle in the Gilson ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems. The addition of the ASPEC-compatible caps allows the positive pressure applied by the Gilson instruments to aspirate the sample and solvents through the Bond Elut SPE cartridges. Varian supplies the cartridges precapped from our factory, and in large package quantities to provide a cost-effective ready-made solution. Also, the caps and standard Bond Elut columns may be purchased separately. See the Bond Elut Accessories section.


ASPEC Capped SPE Bond Elut Cartridges

Sorbent Phase

Sorbent Mass(mg)

1 mL 3 mL 6 mL Quantity(/Pk)

C18

50 22102058 500100 22102001 500200 22102025 500500 22102028 22102052 500

C2

50 22102060 500100 22102003 500200 22102027 500500 22102030 500

C8

50 22102059 500100 22102002 500200 22102026 500500 22102029 22102053 500

CBA50 22102073 500100 22102011 500500 22102038 500

CN-E50 22102066 500100 22102008 500500 22102035 500

Certify130 22102051 500300 22102081 22102082 500

Certify II 200 22102080 500

NH250 22102076 500100 22102014 500500 22102041 500

SAX50 22102079 500100 22102017 500500 22102044 500

SCX50 22102075 500100 22102013 500500 22102040 500

Silica50 22102068 500100 22102010 500500 22102037 500

ASPEC Caps• Convert Bond Elut 1, 3 and 6 ml cartridges for use in Gilson SPE systems.

These special caps produce a positive pressure seal with the needle in the Gilson ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems, and allow the positive pressure applied by the Gilson instruments to aspirate the sample and solvents through Bond Elut SPE cartridges.

Also, we sell a variety of Bond Elut cartridges with these caps preinstalled, ready for use. See the Bond Elut Cartridge SPE section.

ASPEC Caps for SPE Cartridges


Gilson Adapter 1 mL, Yellow 12131034 1000Gilson Adapter 3 mL, Blue 12131035 1000Gilson Adapter 6 mL, Red 12131036 1000





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NEXUS• Unique, ultra-clean copolymeric material• No conditioning needed for hydrophobic analytes• High flow for viscous samples• Ideal for veterinary sciences and environmental applications

Nexus provides simple, clean SPE on a unique polymeric sorbent. It offers a nonpolar retention mechanism with no conditioning required, enabling increased throughput or improved ease of field sampling in environmental applications.

Nexus delivers reproducibly high recoveries. This performance is achieved from the use of a proprietary, highly cross-linked, spherical polymeric sorbent that can bind both polar and non-polar analytes. The large particle size makes Nexus ideal for extracting highly viscous samples such as horse urine.


NEXUS LRC CartridgesSorbent

Mass(mg)


(/Box)

30 10 12113100 50

60 10 12113101 50

NEXUS Straight Barrel CartridgesSorbent

Mass(mg)


(/Box)

30 1 12103100 100

60 3 12103101 100

2006 12103102 30

12 12253101 20

50012 12253102 20

20 12253103 20

Nonpolar Application Development Kits For more convenient and economical method de-velopment, Varian offers Bond Elut Application De-velopment kits for sorbent screening. Three types of Application Development kits are available: nonpolar, polar/ion exchange, and

polymer/silica C18. An instruction manual outlining fundamental extraction considerations is included with each kit.

The nonpolar Application Development Kit contains these Bond Elut silica-based sorbents: C18, C8, C2, CH, PH, and CN-U. The 100 mg kit includes 10 cartridges of each sorbent. The 500 mg kit includes 5 cartridges of each sorbent.


Nonpolar Application Development KitsC18, C8, C2, CH, PH, and CN-U Bond Elut cartridges, 10 each

Sorbent Mass(mg)


(/Pk)

100 1 12102054 60500 3 12102055 30

Polar/ Ion Exchange Application Development Kits

The Polar/Ion Exchange Application Development Kit contains these Bond Elut silica-based sorbents: CBA, NH2, 2OH, SAX, SCX, and SI. The 100 mg kit includes 10 cartridges of each sorbent. The 500 mg kit includes 5 cartridges of

each sorbent. A useful instruction manual outlining fundamental extrac-tion considerations is included with each kit.


Ion Exchange Application Development KitSI, NH2, 2OH, SCX, SAX, and CBA Bond Elut cartridges, 5 or 10 each

Sorbent Mass(mg)


(/Pk)

100 1 12102056 60500 3 12102057 30



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Polymer and C18 Varieties Application Development KitIn response to the popularity of high-throughput screening, Varian of-fers a polymer and C18 method development kit that contains our wide range of C18 silicas and nonpolar resins. In addition, both the standard (40 µm) particle size and a high flow (120 µm) C18 phase have been included. This kit will allow labs seeking to validate screening methods on nonpolar sorbents to find the best sorbent option. Each phase is packed into 1 mL straight barrel Bond Elut cartridges, which are compatible with robotic systems. Standard 20 µm, polyethylene frits are used. The 100 mg bed masses are optimized for smaller samples and elution volumes. The sorbents included in this kit are:

C18 - Our most widely used nonpolar sorbent: New applications are continually being developed on Bond Elut C18.

C18 HF -This 120 µm high flow sorbent is effective for viscous or particulate-laden samples, but also offers the added selectivity of silanols on the silica substrate.

C18 EWP - Extra wide pore size (500 Å) allows for better retention of macromolecules, proteins, and other large molecules typically excluded from standard 60 Å pores.

C18 OH - Monofunctional bonding provides slightly lower carbon loading, enhanced polar characteristics, and greater access to silanols, resulting in a unique selectivity for nonpolar analytes.

PPL - A functionalized polystyrene-divinylbenzene polymer (PSDVB), PPL is our most nonpolar sorbent. It is effective in extracting the most polar organic compounds from aqueous matrices.

ENV - The larger particle size of this pure PSDVB resin makes ENV useful for particulate-laden samples. In addition, it is useful for applica-tions requiring extremes of pH.


Polymer and C18 Varieties Application Development KitC18, C18 EWP, C18 OH, C18 HF, PPL and ENV Bond Elut cartridges, 10 each

Sorbent Mass(mg)


(/Pk)

100 1 12102154 60

Sodium Sulfate Drying CartridgesSimplify your sodium sulfate drying step by using cartridges prepacked with ACS grade, granular anhydrous sodium sulfate. Both the Bond Elut LRC and the Bond Elut Jr. configurations are available. Bond Elut Jr. cartridges have Luer fittings top and bottom for easy parallel processing in conjunction with standard SPE cartridges. Bond Elut LRC cartridges have a large reservoir above the drying bed and are suitable for use on any standard SPE vacuum manifold.

Sodium Sulfate Drying CartridgesSorbent

Mass(g)

Column Format


(/Pk)

1 LRC 10 12131033 100

1.4 Bond Elut Jr. N/A 12162052B 100

2.2 Bond Elut Jr. N/A 12162054B 100

3 Bond Elut Jr. N/A 12162051B 100

15 Straight Barrel 60 12132004 16

Bond Elut Accessories


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Empty SPE Cartridges• Available in sizes from 1 to 60 ml• Made with clean polypropylene for clean extracts• Uniform batch-to-batch size for consistent performance• Economical

A variety of empty reservoirs are available to increase the sample capacity of Bond Elut extraction cartridges. Also, empty reservoirs may also be purchased with frits and used to pack custom SPE cartridges with bulk Bondesil sorbents or other customer-specified packing.

Empty SPE CartridgesOrder frits separately, or order our cartridges with preinstalled frits.

Volume 1 mL 3 mL 6 mL 12 mL 20 mL 60 mLPart No. Price 12131007 12131008 12131009 12131010 12131011 12131012

Quantity 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk

Empty SPE Cartridges with 2 Frits• Available in sizes from 1 to 60 ml• 2 polypropylene frits preinstalled• Made with clean polypropylene for clean extracts

These extra-clean polypropylene reservoirs contain 2 polyethylene frits preinserted into the tubes. This is an ideal configuration for simple filtration. For custom sorbent packing, additional frits can be purchased separately.

Empty SPE Cartridges with 2 FritsVolume 1 mL 3 mL 6 mL 12 mL 20 mL 60 mL

Part No. Price 12131013 12131014 12131015 12131016 12131017 12131018 Quantity 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk

Frits for SPE Cartridges• 20 µm polyethylene frits for SPE cartridges• Made with clean polyethylene for clean extracts

Clean 20 µm polyethylene frits are precut to fit into Bond Elut reservoirs for either filtration or custom packing needs.

Frits for SPE CartridgesColumn Format 1 ml 3 ml 6 ml 12 ml 20 ml 60 ml

Part No. Price 12131019 12131020 12131021 12131022 12131023 12131024 Quantity 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /PkDiameter 1/4 in. 3/8 in. 1/2 in. 5/8 in. 13/16 in. 1-1/16 in.

Bond Elut Accessories


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Luer Stopcocks• Luer stopcocks for SPE cartridges• Control flow rates of individual cartridges

Luer stopcocks are used to provide independent flow control of each individual Bond Elut cartridge when used with vacuum manifolds. They are made of solvent-resistant polypropylene, are reusable, and are readily cleaned.

Luer Stopcocks for SPE Cartridges

Description Part No. Quantity(/Pk)

Luer Stopcocks 12131005 15

ASPEC Caps• Convert Bond Elut 1, 3 and 6 ml cartridges for use in Gilson SPE systems.

These special caps produce a positive pressure seal with the needle in the Gilson ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems, and allow the positive pressure applied by the Gilson instruments to aspirate the sample and solvents through Bond Elut SPE cartridges.

Also, we sell a variety of Bond Elut cartridges with these caps preinstalled, ready for use. See the Bond Elut Cartridge SPE section.

ASPEC Caps for SPE Cartridges


Gilson Adapter 1 mL, Yellow 12131034 1000

Gilson Adapter 3 mL, Blue 12131035 1000

Gilson Adapter 6 mL, Red 12131036 1000

Bond Elut® Adapters• Connect SPE cartridges in series• Expand cartridge volume• Transfer large-volume samples to any SPE cartridge• Add versatility to your SPE processing

When a sample volume exceeds the capacity of a Bond Elut cartridge reservoir, you can increase the volume capacity by attaching a larger reservoir to the top of the smaller cartridge using a Bond Elut adapter. Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge. This allows you to:

• Stack cartridges for custom multi-sorbent methods (#1 in the photo). • Increase any cartridge’s volume capacity by stacking an empty reservoir

on top of the cartridge (#2, 3). • Use a standard Luer-tipped syringe to apply positive pressure for

processing samples (#4). Luer-tipped syringes fit into any Bond Elut adapter. Gentle pressure is all that is needed to apply conditioning solvents, samples, rinse solutions, and elution solvents.

• Pull large-volume samples through any SPE cartridge (#5). Simply insert one end of a 1/8” OD tube through the opening in the Bond Elut adapter (forming a seal), and insert the other end of the tube into the sample container. Then apply a vacuum to the SPE cartridge to pull the entire sample through.

To select the appropriate adapter, choose the adapter specified for the size of the lower Bond Elut cartridge. The adapter fits snugly into the top of the cartridge reservoir. All Bond Elut cartridges and empty SPE cartridges have a standard Luer tip that is usable with these adapters.

Bond Elut Cartridge Adapters

Description Part No. Quantity(/Pk)

Adapter caps for 1, 3 and 6 mL Bond Elut cartridges 12131001 15

Adapter caps for 12 mL & 20 mL Bond Elut cartridges, and LRC cartridges 12131003 10

Adapter caps for 60 mL Bond Elut cartridges 12131004 10

Envir Elut 1664


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EnvirElut® 1664• The best available system for Oil and Grease analysis• Developed for EPA Method 1664• Each lot pretested using EPA protocol• Extra low level blanks• High-flow configuration• One-of-a-kind, totally enclosed extraction system

Varian’s EnvirElut1664 Oil & Grease solid phase extraction processing system is ideal for monitoring waste water, aqueous effluent, and surface/ground water. It is faster, more efficient, and uses less solvent than sample preparation performed with liquid/liquid extraction. EnvirElut 1664 cartridges consistently pass 1 liter dirty water samples in less than 10 minutes without loss of analyte, delivering greater than 90% recovery with oil and grease standards from 5 mg/L to 1 g/L. Our cartridge design has been optimized for maximum flow of large-volume samples, and the use of depth filters ensures that even particulate-laden samples are processed without clogging. The procedure is entirely Freon-free, and offers a solid phase extraction alternative with results equivalent to EPA 1664/ASTM Method 413.1. EnvirElut 1664 cartridges and the EnvirElut 1664 manifold were specifically designed to work together, and the 1664 cartridges are not compatible with Vac Elut or other manifolds.

EnvirElut 1664 Cartridges

Item Name Part No. Quantity(/Box)

EPA 1664 Oil & Grease Cartridges with AquaSep Cartridges 12272020 20

EnvirElut® 1664 Manifolds

A one- and a six-station manifold system are available to accommodate the number of samples you need to process. Each manifold system comes complete with necessary tubing, vials, and sampling container. In addition to the replacement components, Varian offers an easy pour, open top, 1 L glass sample reservoir that can be used in place of the standard glass sample bottle and 89 mm cap. This reservoir ensures that a full 1 L sample can easily be loaded and processed, and is compatible with both systems.

EnvirElut 1664 Manifolds


Single Station EnvirElut Manifold, Complete System 12235101 1

Six Station EnvirElut Manifold, Complete System 12235102 1

EnvirElut Manifold Replacement Parts


1 Liter Glass Sample Reservoir 12235157 1

50 mL Collection Tube 12235154 1

89 mm, Adapter Cap for Sample Bottle 12235151 1

89 mm, Glass Sample Bottle 12235153 1

EnvirElut Manifold Viton O-ring 12235155 1

Glass Collection Chamber 12235152 1

Two Place Elution Station Replacement 12235156 1

Bond Elut 96


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Bond Elut® 96• Available with many of our most popular Bond Elut sorbents• Fast revalidation of cartridge to 96-well SPE methods• Low-profile, automation-friendly 96-well design

The same trusted Varian silica-based sorbents used in our standard Bond Elut cartridge products are now available in the streamlined new Bond Elut 96 Well Plates. Choose Bond Elut 96 for fast revalidation of existing cartridge-based methods to a 96-well format. Bond Elut 96 components are specially formulated to offer superior cleanliness, flow reproducibility, and reliability. And because we manufacture in-house, fast delivery times are standard. Varian, Inc. also offers complementary 96-well SPE products, SPEC and Focus. SPEC 96-well SPE plates provide the low elution volumes and superior extraction efficiency characteristic of monolithic disc-based SPE. Focus 96-well SPE plates offer a unique polar-enhanced SPE phase ideal for preparing biofluid samples for LC/MS analysis with minimal ion suppression and overall cleaner extracts.

Bond Elut 96 Plates with Silica-based Sorbents

Sorbent Phase 25 mg 50 mg 100 mgParticle

Size(µm)

Quantity(Each)

C18 A4960150 A496011C 40 1

C18/OH A496291C 40 1

C2 A4961125 A4961150 A496111C 40 1

C8 A4960325 A4960350 A496031C 40 1

CBA A4960625 A4960650 A496061C 40 1

CH A4962225 A4962250 A496221C 40 1

CN-E A4960425 A4960450 A496041C 40 1

CN-U A4961425 A4961450 A496141C 40 1

Certify A4960925 A4960950 A496091C 40 1

NH2 A4960525 A4960550 A496051C 40 1

PBA A496121C 40 1

PH A496151C 40 1

PSA A4962825 A4962850 A496281C 40 1

SCX A4960725 A4960750 A496071C 40 1

Bond Elut 96 Plates with Polymeric Sorbents

Sorbent Phase 10 mg 30 mgParticle

Size(µm)

Quantity(Each)

LMS A4961010 75 1

Nexus A4962030 70 1

Did you know you can convert your straight-barrel Bond Elut application to 96-well format with Bond Elut 96?

VersaPlate 96-well SPE


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VersaPlate® Well Plate Tubes• Available in a variety of sorbents, bed masses, and particle sizes• Customize your own 96-well plate• Eliminate needless and costly waste• Streamline your SPE method development process

Varian can take you to the next generation of increased lab productivity, targeted at improving method development and optimization in a 96-well plate configuration. Method development in single block well plates can be costly because screening a variety of sorbents or optimizing bed mass can be accomplished only with expensive, custom-ordered plates, or by wasteful use of standard plates.

Surveys show that on average 30% of the wells of an SPE plate are unused during the method development process.

Varian has solved this problem with its innovative VersaPlate system. VersaPlate allows you to:- customize your own 96-well plate- eliminate needless and costly waste- streamline your SPE method development process

Individual cartridges snap into a 96-well configuration, allowing you to create a multi-sorbent plate quickly and easily. Multiple sorbents can then be rapidly screened for effectiveness in an assay. Or, different bed masses of the same sorbent can be evaluated for maximum capacity and minimum elution volume.

For even greater flexibility, the individual VersaPlate tubes are designed to fit our standard Vac Elut SPE vacuum manifold. This allows you to perform manual extractions with the confidence that you can scale up to high-throughput automation with no additional method modification. And when your method is optimized, you can purchase preassembled plates for maximum convenience.

For technical information on the available chemistries, refer to the Sorbent Selection Guide on pages 5-11.

Order the VersaPlate base plate separately.

VersaPlate Tubes - Silica Sorbents96/pack

Sorbent PhaseParticle

Size(µm)

25 mg 50 mg 100 mg

C1840 75501025 75501050 7550101C 120 75502050

C18HF 120 75502025 7550201C C2 40 75511025 75511050 7551101C C8 40 75503025 75503050 7550301C

CBA 40 75506025 75506050 7550601C CN-E 40 75504025 75504050 7550401C CN-U 40 75514025 75514050 7551401C Certify 40 75509025 75509050 7550901C

Certify II 40 75518025 75518050 7551801C DEA 40 75517025 75517050 7551701C NH2 40 75505025 75505050 7550501C PBA 40 7551201C PH 40 75515025 75515050 7551501C SAX 40 75508025 75508050 7550801C SCX 40 75507025 75507050 7550701C SI 40 75513025 75513050 7551301C

VersaPlate Tubes - Polymeric Sorbents96/pack

Sorbent Phase 10 mg 25 mg 30 mg 50 mg 60 mgLMS 75510010 75510025 75510050

NEXUS 75520010 75520030 75520060

VersaPlate Tubes - HydromatrixPurify organic reaction mixtures, or perform bioanalytical liquid/liquid extractions using Varian’s Hydromatrix brand diatomaceous earth, now available in the VersaPlate format. The 96-well format with 260 mg of Hydromatrix per well allows for extraction of up to 500 uL of biofluid samples, or purification of 96 parallel reactions of up to 2 mL in volume. 96/pack

Sorbent Phase 260 mgHydromatrix 75530260

VersaPlate 96-well SPE


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VersaPlate® Base Plate• Customize your own 96-well plate• Eliminate needless and costly waste• Streamline your SPE method development process

The VersaPlate 96-well base plate accepts VersaPlate cartridges in any configuration you need, allowing you to create a multi-sorbent plate quickly and easily. Multiple sorbents can then be rapidly screened for effectiveness in an assay. Or, different bed masses of the same sorbent can be evaluated for maximum capacity and minimum elution volume.

Order VersaPlate tubes separately.

VersaPlate Base Plate

Description Part No. Quantity(Each)

VersaPlate Base Plate only 75400000 1

VersaPlate® Preassembled Plates• Modular 96-well plate design• Reduces operating costs• Streamlines the SPE method development process

Individual VersaPlate cartridges snap in to a special 96-well base plate that gives you the power to customize your 96-well plate SPE. Develop new methods faster by building your own multisorbent plate to rapidly screen different sorbents and bed masses. Reduce needless and costly waste when processing small sample volumes by configuring a plate with the exact number of cartridges you need, or process large numbers of samples effortlessly by purchasing preassembled plates.

For technical information on the available chemistries, refer to the Sorbent Selection Guide on pages 5-11.

VersaPlate Preassembled Plates with Hydromatrix and-Polymer Sorbents


Size(µm)

Sorbent Mass(mg)

Part No.

Hydromatrix 260 75430260

LMS

75 10 75410010 75 25 75410025 75 50 75410050

NEXUS

70 10 75420010 70 30 75420030 70 60 75420060

VersaPlate® Manifolds & AccessoriesKeep a supply of replacement parts on hand so your VersaPlate system is always ready to use.

Description Part No. QuantityVersaPlate manifold, complete, acrylic/acetal 12236101 1 Each

VersaPlate manifold top, clear acrylic 12236102 1 EachVersaPlate manifold bottom, white acetal 12236103 1 Each

VersaPlate manifold complete shim set 12236104 1 Each

VersaPlate manifold bottom gasket 12236105 1 Each

VersaPlate manifold top gasket 12236106 1 Each

VersaPlate 8-well sealing strip 12236108 240 /Pk

VersaPlate Preassembled Plates with Silica-based Sorbents


Size(µm)

25 mg 50 mg 100 mg

C18 40 75401025 75401050 7540101C C18HF 120 75402025 75402050 7540201C

C2 40 75411025 75411050 7541101C C8 40 75403025 75403050 7540301C

CBA 40 75406025 75406050 7540601C CN-E 40 75404025 75404050 7540401C CN-U 40 75414025 75414050 7541401C Certify 40 75409025 75409050 7540901C

Certify II 40 75418025 75418050 7541801C DEA 40 75417025 75417050 7541701C NH2 40 75405025 75405050 7540501C PBA 40 7541201C PH 40 75415025 75415050 7541501C SAX 40 75408025 75408050 7540801C SCX 40 75407025 75407050 7540701C SI 40 75413025 75413050 7541301C

Bond Elut Matrix 96-Well SPE


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Bond Elut® Matrix 96-Well Plates• One-piece design• Square wells


Sold 1 plate each.

Bond Elut Matrix 96-Well Plates - Silica Sorbents

Sorbent Phase Particle Size(µm) 10 mg 15 mg 25 mg 50 mg 100 mg

C1840 5801000 5801001 5801002 5801003 5801004

120 5802000 5802001 5802002 5802003

C18 OH 40 5801040 5801041 5801042 5801043 5801044

C2 40 5801020 5801021 5801022 5801023 5801024

C840 5801010 5801011 5801012 5801013 5801014

120 5802010 5802011 5802012 5802013

CBA 40 5801080 5801081 5801082 5801083 5801084

CN-E 40 5801030 5801031 5801032 5801033 5801034

CN-U 40 5801050 5801051 5801052 5801053 5801054

Certify40 5801110 5801111 5801112 5801113 5801114

120 5802110 5802111 5802112 5802113

Certify II40 5801120 5801121 5801122 5801123 5801124

120 5802120 5802121 5802122 5802123

NH2 40 5801060 5801061 5801062 5801063 5801064

Phenyl 40 65401144 65401145 65401146 65401147 65401148

SAX 40 5801100 5801101 5801102 5801103 5801104

SCX 40 5801090 5801091 5801092 5801093 5801094

Silica 40 5801070 5801071 5801072 5801073 5801074

Bond Elut Matrix 96-Well Plates - Polymeric Sorbents

Sorbent Phase Particle Size(µm) 10 mg 15 mg 25 mg 30 mg 50 mg 60 mg

LMS 75 5801130 5801131 5801132 5801133

NEXUS 70 65401150 65401151 65401152 65401153


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Bond Elut® Matrix Collection PlatesThe Bond Elut Matrix manifold system also has a range of collection plates and manifold accessories. Collection plates in 0.35, 1 and 2 mL for the square well configuration as well as 1 and 2 mL round well collections plates are available and compatible with both manifold types. Varian can provide sealing caps for the square and round well collection plates.

Empore Sealing Tape is also compatible with the 96 well plates and manifold system (see Empore Disk Plate section).

Bond Elut Matrix Collection PlatesShape Volume Part No. Quantity Item Name

Round1 mL 5132101 50 /Box

Round 96- Well Collection Plate, 1 mL - 50/box

2 mL 5801505 1 EachEmpty 96-Well Plate with Frits

Square

350 µL 5133007 50 /BoxSquare 96- Well Collection Plate, 350 µL - 50/box

1 mL 5133008 50 /BoxSquare 96- Well Collection Plate, 1 mL - 50/box

2 mL 5133009 50 /BoxSquare 96- Well Collection Plate, 2 mL - 50/box

Sealing Caps for Round Well Plates

Shape Part No. Quantity(/Box) Item Name

Round 5132200 5096-Well Plate Round Well

Sealing Caps - 50/box

Bond Elut Matrix Protein Precipitation Plate

Protein Precipitation

Part No. Quantity(Plate)

65932003 1

Bond Elut® Matrix Manifolds & AccessoriesThe Bond Elut Matrix system’s lightweight acrylic vacuum manifold features a transparent top for easy viewing of sample and solvent application steps. The one-piece integrated design offers leak-free flow and virtually eliminates cross-contamination. A deep-well anodized aluminum manifold is also available which offers a more durable, rugged and solvent resistant format.

96-Well Plate Manifold Replacement ComponentsItem Name Part No. Quantity

Drain Cap - 25/box 5133006 25 /Box

Profile Gasket for Acrylic Manifold 5133101 1 Each

Flat Gasket for Acrylic Manifold 5133102 1 Each

Gasket for Aluminum Manifold 5231101 1 Each

96-Well Plate Manifolds


96 Well Acrylic Manifold 5133000 1Aluminum Manifold with the Deep Well Collection Plate 5231002 1


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Prospekt Cartridges

Prospekt cartridges are available from Varian packed with the complete range of Bondesil bonded silica and polymer based sorbents, known worldwide for their superior performance and highly reproducible chemistries. Varian offers the 2 mm Prospekt cartridges for all of the standard phases, and the new 1 mm format for a select number of sorbents. The 2 mm cartridges offer the benefits of higher capacity and higher flow rates. The 1 mm configuration offers sharper band resolution due to less dead volume and lower elution volumes, making them ideal for direct MS applications.


Prospekt Cartridges

Sorbent Phase 1 mm 2 mm Quantity(/Box)

2OH 12221010 100C18 12221024 12221001 100

C18 OH 12221031 12221008 100C2 12221026 100C8 12221025 12221002 100

CBA 12221039 12221016 100CH 12221029 12221006 100

CN-E 12221030 12221007 100CN-U 12221032 12221009 100

Certify II 12221102 100DEA 12221021 100

EnvirElut 12221103 100Focus 12221301 96NEXUS 12221302 96NH2 12221042 12221019 100PH 12221028 12221005 100

PLRP-S 12221201 100PRS 12221017 100PSA 12221020 100SAX 12221022 100SCX 12221018 100

Prospekt Method Development Kits

Item Name Sorbent Phase 2 mm Quantity(/Box)

Prospekt Anion Exchange Application Development Kit #1 Certify II, SAX, PSA, DEA, NH2, 20 each, 20/phase 12222005 100

Prospekt Anion Exchange Application Development Kit #2 SAX, DEA, PRS, CBA, 25 each, 25/phase 12222007 100

Prospekt Cation Exchange Application Development Kit SCX, PRS, CBA, Certify, 25 each, 25/phase 12222004 100

Prospekt Nonpolar Application Development Kit #1 C18, C8, C18-OH, EnvirElut, 25 each, 25/phase 12222001 100

Prospekt Nonpolar Application Development Kit #2 PH, CH, C2, CN-E, 25 each, 25/phase 12222002 100

Prospekt Nonpolar Application Development Kit #3 C18, C8, C2, CN-E, 25 each, 25/phase 12222006 100

Prospekt HardwareA Prospekt Rapide filling tool is the newest hardware addition. This unit allows the user to snap cartridges into the Prospekt cartridge strips fast and easily. Simply drop the cartridges into the hollow handle, clip the handle plate on, insert the cartridge strip and twist the handle.

Prospekt Hardware


Prospekt Rapide Filling Tool 12223001 1

Captiva Filtration Products


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Captiva® 96-well Filter Kits• Faster than centrifugation • Easily automated • No plugging or interference• No loss of analytes or nonspecific binding • Excellent for sample storage • Avoid fibrinogen clogging forever• Everything you need to start or replenish your Captiva filtration systems

Captiva’s unique dual depth filtra-tion media provides both complete removal of precipitated proteins and outstanding resistance to sample clogging. All Captiva components are ultraclean, and rigorously tested to ensure against nonspecific binding. With Captiva, your plasma samples are processed quickly and reliably. Starter kits contain everything you need. Replacement kits include

everything you need to replenish your Captiva system, but do not include the CaptiVac vacuum collar.

Captiva 96-well Starter Filter KitsEach kit contains:1 CaptiVac Vacuum Collar5 Captiva Filter Plates10 Duo Seal 96 96-well plate seals5 Captiva 96-Deep Well 1 mL Collection Plates5 Captiva collection plate pierceable covers

Pore Size(µm) Filter Material Part No. Quantity

(Kit)0.2 Polypropylene A5960002SK 1

0.45PVDF & Polypropylene A5967045SK 1

Polypropylene A5960045SK 1

0.6 Polypropylene A5960006SK 1

10 Glass Fiber A596401000SK 1

20 Polypropylene A596002000SK 1

PVDF = polyvinyldifluoride

Captiva Replacement KitsEach kit contains:5 Captiva Filter Plates10 Duo Seal 96 96-well plate seals5 Captiva 96-Deep Well 1 mL Collection Plates5 Captiva collection plate pierceable covers

Pore Size(µm) Filter Material Part No. Quantity

0.2 Polypropylene A5960002K 1 Kit

0.45PVDF & Polypropylene A5967045K 1 Kit

Polypropylene A5960045K 1 Kit

10 Glass Fiber A596401000K 1 Kit

20 Polypropylene A596002000K 1 Kit

Captiva® 96-well Filter Plates• 10 µm and 20 µm filtration plates for plasma and other particle-laden

samples• 0.2 µm and 0.45 µm filtration plates for precipitated proteins and other

samples• Clean and clear filtrates• High analyte recovery• No nonspecific adsorption• Perfect for automated systems• For use with Duo Seal 96 96-well seals

Captiva 96-well filter plates are offered in several versions.

The Captiva 0.2 µm and 0.45 µm depth filter plates are ideal to prepare precipitated protein samples for LC/MS analysis. Filtration is simple, versatile, and necessary to prevent clogging of expensive HPLC columns.

The Captiva 10 µm and 20 µm glass fiber filter plates are designed for clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample transfer problems due to pipette tip clogging.

Captiva 96-well Filter PlatesPore Size

(µm) Filter Material Part No. Quantity

0.2 Polypropylene A5960002 5 /Pk

0.45PVDF & Polypropylene A5967045 5 /PkPolypropylene A5960045 5 /Pk

10 Glass Fiber A596401000 5 /Pk

20Polypropylene A596002000 5 /PkPolypropylene A596002000B 100 /Pk


Captiva Filtration Products


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Captiva® Filter CartridgesCaptiva filter cartridges bring all of the benefits of Captiva filtration to the standard SPE cartridge format. The 0.2 µm and 0.45 µm depth filter cartridges are ideal to prepare precipitated protein samples for LC/MS analysis. The Captiva 10 µm glass fiber filter cartridge is designed for clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample transfer problems due to pipette tip clogging.

Captiva Filter CartridgesPore Size

(µm) Filter Material Volume(mL) Part No. Quantity

0.2 PVDF & Polypropylene 3 A5300002 100 /Pk

0.45 PVDF & Polypropylene 3 A5307045 100 /Pk

10 Glass Fiber 10 A500401000 100 /Pk


CaptiVac™ Vacuum Collars• For use with Captiva Filtration and 96-Deep Well collection plates, and

SPEC SPE 96-well plates.• Prevents cross-contamination• Simple to use• Cost effective

This patented vacuum collar is a completely transparent device that joins Captiva filtration or SPEC SPE 96-well plates directly to our collection plate. The unique design of the CaptiVac collar forms a preset, prealigned vacuum seal between the SPE or filtration device and the collection plate, which positions the outlet tips a specified distance into the collection plate to prevent cross-contamination of samples. The device is so compact, simple, and cost effective that a lab can be outfitted with many of these collars for the cost of a single standard manifold. CaptiVac makes 96-well SPE and filtration simple, practical, and affordable for any size laboratory.

CaptiVac Vacuum CollarsDescription Part No. Quantity

CaptiVac Vacuum Collar A796 1CaptiVac Vacuum Collar for Hamilton MICROLAB ATplus 2 A796H 1

CaptiVac Vacuum Collar AccessoriesDescription Part No. Quantity

CaptiVac Gasket Kit A796G 5

Captiva® 96-well Collection Plates & Cover• 1 mL 96-deep well collection plates for Captiva filtration and SPEC SPE

applications• Pierceable 96-well silicone collection plate covers

Captiva 96-deep well collection plates are specially designed for use with Captiva filtration plates and SPEC SPE solid phase extraction 96-well plates. The 1 mL capacity provides the volume needed to collect all of your filtrate or eluate.

Captiva Pierceable 96-well silicone covers are easily applied to completely seal our collection plates. This ensures no sample loss due to spillage or evaporation, and no sample contamination. The pierceable silicone is specially designed for 96-well auto injectors providing easy piercing and removal with no “coring”.

Captiva 96-well Collection Plates & CoverDescription Part No. Quantity

Captiva 96-Deep well Collection Plate A696001000 10Captiva 96-Deep well Collection Plate for Hamilton MICROLAB ATplus 2 A696001000H 10

Captiva Pierceable 96-well Collection Plate Cover A8961007 10

Varian Syringe Filters


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Varian Syringe Filters• Available in 4, 17 and 30 mm diameters• Pore sizes from 0.2 to 1.2 µm• Nylon, PTFT, polypropylene, regenerated cellulose and PVDF filter materials• Available with glass fiber prefilters• Hydrophobic and hydrophilic membranes• Low and high protein binding membranes

A wide variety of disposable syringe-tip filters are available for applications requiring the highest quality membrane-based devices. Use the following selection guide for selecting the appropriate syringe filter materials:

Hydrophilic Nylon — naturally hydrophilic, high protein binding, high mechanical stability, and high dirt loading capacity. Often used for samples directly injected into HPLC sample loops.

Polypropylene (PP) — hydrophilic, low protein binding, and designed for aqueous and organic solvent based samples. This filter has low extractables and provides exceptionally low detection levels.

Regenerated Cellulose (RC) — hydrophilic, solvent-resistant membrane. It is a very low protein binder and is ideal for filtration of proteins, peptides and other biomolecules.

PVDF (polyvinylidene difluoride) — hydrophilic membrane suitable for most general-purpose filtration applications. It is a low protein binder and has a broad chemical compatibility.

Hydrophobic PTFE (Teflon®) — hydrophobic, low protein binding, recommended for HPLC solvent or gas chromatography filtration, aggressive solutions, and small venting applications. Requires prewetting for aqueous samples

Glass fiber prefilters are recommended when samples have high particulate matter to reduce filter bed clogging.

Popular sizes are available in 500 filter packs.

Varian Syringe FiltersDiameter

(mm) Filter Material Prefilter Quantity(/Pk) 0.2 µm 0.45 µm 1 µm 1.2 µm

4

Nylon No 100 A4101 A410 PTFE No 100 A4103 A4102 PVDF No 100 A4136 RC No 100 A4140 A4139

17

Nylon No 200 A4131 A4130

PTFE No200 A4135 A4133 500 A4170

PVDF No 200 A4137 RC No 200 A4142 A4143

30

Nylon

Glass Fiber 100 CP201939 CP201940

No100 A4105 A4104 A4109 500 A4132

PP No 100 CP201944 CP201945

PTFE

Glass Fiber 100 CP201950 CP201951

No100 A4107 A4106 A4129 500 A4134

PVDFGlass Fiber 100 CP201955

No 100 A4138 RC No 100 A4145 A4144

Varian Syringe Filters


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Combilute Combilute is a 96-well SPE plate filled with Hydromatrix-brand diatomaceous earth. Each well is filled with approximately 250 mg Hydromatrix and sealed with a piercable sealing mat.

The product was specifically designed for drug discovery and pharmacokinetics laboratories performing:• Purification of combinatorial libraries• Screening for pharmaceuticals from biological fluids

Combinatorial Chemistry:Combilute is an ideal purification tool for combinatorial libraries. Remove polar reaction byproducts, reagents, and impurities. No vacuum manifold is required and the use of any standard robotic liquid handler can be used.

Pharmaceutical Drug Screening:Drug screening of plasma or other biological fluid samples can be efficiently performed with Combilute. In studies where a large number of patient samples must be quickly analyzed, the 96-well format of Combilute is indispensible. Robotic liquid handling systems, already used for many other functions in pharmaceutical labs, can be easily adapted to dispense 96 samples quickly into a single Combilute plate, positioned on top of a deep well collection plate. No vacuum manifold is required. And the entire procedure, from sample application to elution, can be easily and fully automated.

Sorbent Mass(mg) Sorbent Phase Part No. Quantity

(Plate)200 Hydromatrix 65401507 1

Chem Elut™

• Great for purifying organic synthesis mixtures• Simple method for bioanalytical sample preparation• No emulsions• Gravity flow• Buffered and nonbuffered versions available• Automatable

Chem Elut cartridges are ideal for purification or sample preparation.

For sample preparation of biological samples, select the cartridge which fits the total volume of the sample (including any buffers or additives). Simply load the sample; wait 3-5 minutes; then elute with organic solvent. This general method is ideal for LC/MS sample preparation.

For organic reaction mixture purification, simply load the cartridge with aqueous acid to scrub amines (or add base to scrub acids). Select cartridge size based on the total volume of aqueous buffer to be used. Then pass the crude synthesis mixture through the cartridge. Gravity flow is all that’s needed – no vacuum manifolds or pressure required. It’s the fast, easy, reproducible way to scrub organic synthesis mixtures of excess reagents and reaction byproducts!

Buffered pH Volume(mL) Part No. Quantity

(/Pk)

4.53 12198004 100

100 12198018 25

9.0 3 12198005 100

Unbuffered

0.3 12198001 100

1 12198002 100

3 12198003 100

5 12198006 100

10 12198007 100

20 12198008 100

50 12198009 50

100 12198010 25

300 12198011 15

Hydromatrix™

• High purity diatomaceous earth• Available in straight-barrel SPE cartridges• Available in 96-well plates• Available in VersaPlate format• Available in bulk Hydromatrix is a high-purity, inert diatomaceous earth available in a variety of convenient configurations with a multitude of timesaving uses.

Chem Elut products contain Hydromatrix in convenient, ready-to-use cartridges. Chem Elut is ideal for rapid, general sample preparation of bio-analytical samples (plasma, serum, whole blood, urine). Chem Elut can also be used for purifying organic synthesis mixtures.

For 96-well sample prep or reaction work-up, Combilute contains Hydromatrix in a standard 96-well plate. Hydromatrix is also available in bulk quantities for extra large-scale purification, or for SFE or ASE applications.

Item Name Quantity Part No.

Hydromatrix1 kg 198003 4 kg 198004

Combilute 96-well Plate, Hydromatrix 1 Plate 65401507 Versaplate 96-well-Hydromatrix, 260 mg 1 Plate 75430260 VersaPlate Well Plate Tubes, HYDROMATRIX, 260 mg, 96/pk

96 /Pk 75530260

Did you know Chem Elut Cartridges are packed with Hydromatrix brand diatomaceous earth?

Flash Chromatography


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MetaFlash® CartridgesGuaranteed trouble-free purificationYour samples and time are valuable. You can save both by purifying your synthesis mixtures on a MetaFlash cartridge. These rugged and dependable cartridges are fully compatible with your Biotage® Flash instruments and provide the added advantage of a guaranteed leak-free design that preserves every drop of your samples.

Your single source for a full range of sorbents and cartridge sizesIn addition to the cartridges listed here, we supply SCX, NH2, Diol, and many other bonding chemistries. Please contact us for details and ordering infor-mation.

Because we maintain a large inventory of MetaFlash cartridges, you don’t have to. We can ship our full line of cartridges in most popular sizes, including 12, 25, 40, 65, 75, and 150 mm cartridges, within 24 hours. For details on how much a just-in-time (JIT) program and single-sourcing can reduce your costs, please contact your local Varian office.

The highest quality sorbents for the highest level of purificationClean, reproducible purification requires superior sorbent. Ultra-clean Varian sorbents provide the lowest residue levels to deliver the highest level of purification. Fast, reproducible flow is achieved with precisely controlled particle sizing. An extensive, well-documented quality program (ISO 9001:2000 certified) ensures lot-to-lot and within-lot reproducibility.

MetaFlash Cartridges - CylindricalSorbent Phase

Diameter(mm)

Sorbent Mass

Length(cm)

Quantity(/Box) Part No.

C18

124 g 7.5 2 A850212S2 8 g 15 2 A850212M2

4045 g 7 1 A850240SE 100 g 15 1 A850240ME

75

200 g 9 1 A850275SE 400 g 15 1 A850275ME 800 g 30 1 A850275LE

1502.5 kg 30 1 A8502150ME 5 kg 60 1 A8502150LE

C8 75 5 kg 60 2 A850375LE

Silica

12

4 g 7.520 A850012S 100 A850012SJ

8 g 1520 A850012M 100 A850012MJ

25

22 g 7.520 A850025S 100 A850025SJ

41 g 1520 A850025M 100 A850025MJ

40

45 g 712 A850040SX 144 A850040SXJ

100 g 1512 A850040MX 144 A850040MXJ

75

200 g 92 A850075S 10 A850075SJ

400 g 152 A850075M 10 A850075MJ

800 g 302 A850075L 10 A850075LJ

150

2.5 kg 302 A8500150M 10 A8500150MJ

5 kg 602 A8500150L 10 A8500150LJ

MetaFlash Cartridges - Nozzle Tip

Sorbent Phase

Diameter(mm)

Sorbent Mass(g)

Length(cm) Quantity Part No.

Silica40

45 712 /Box A850040S

144 /Box A850040SJ

100 1512 /Box A850040M

144 /Box A850040MJ

130 2012 /Box A850040L

144 /Box A850040LJ 65 350 20 6 /Box A850065I

Flash Chromatography


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Mega Bond Elut® Flash• Convenient: disposable Bond Elut cartridges eliminate the need for packing glass columns• Flexible: “open” tube design for both liquid and solid samples• Reliable: consistent, high resolution performance backed by Varian’s ISO 9001 quality systems

Varian’s line of Mega Bond Elut Flash cartridges offers superior levels of performance and productivity for the flash purification of organic compounds from both aqueous and nonaqueous solutions. Prepacked, disposable cartridges offer greater convenience than glass columns that have to be washed, dried and repacked after every run.

Compatibility with parallel automation systems ensures high throughput in cases where maximum productivity is required.

High-yield Separations - milligrams to gramsWith the Varian Mega Bond Elut Flash cartridges, high-yield separations of milligram to gram samples are quickly and easily achieved. Packing materials are based on the Bond Elut 40 µm particles. These cartridges exhibit excellent efficiency due partly to the narrow pore size range of the sorbents.

Varian Mega Bond Elut Flash cartridges are available in a wide range of chemistries. To optimize the purification and economy of your separation, we provide a range of cartridge sizes and sorbent types: Cartridge sizes: 12, 20, 60 and 150 mL Chemistries: C18, NH2, SCX and SI Bed masses: 2g to 70g

Compatibility with Flash Systems - Mega Bond Elut Flash cartridges are compatible with several purification systems including: - Anachem FCM-Single and FCM-10 - Argonaut Flash Master and Flash Pack

Mega Bond Elut Flash Cartridges

Sorbent PhaseSorbent

Mass(g)

Volume(mL) Quantity 40 µm

C18

1 60 16 /Box 12256060 2 12 8 /Box 12256015 5 20 8 /Box 12256023 10 60 8 /Box 12256031 20 60 16 /Box 12256078 25 150 8 /Box 12256079 50 150 8 /Box 12256080 70 150 8 /Box 12256081

NH2

2 12 8 /Box 12256020 5 20 16 /Box 12256028 10 60 16 /Box 12256036 20 60 16 /Box 12256074 25 150 8 /Box 12256075 50 150 8 /Box 12256076 70 150 8 /Box 12256077

SCX

20 60 16 /Box 12256065 25 150 8 /Box 12256070 50 150 8 /Box 12256072 70 150 8 /Box 12256073

SI

2 12 8 /Box 12256018 5 20 8 /Box 12256026 10 60 8 /Box 12256034 20 150 8 /Box 12256042 15 60 16 /Box 12256068 25 150 8 /Box 12256069 50 150 8 /Box 12256067 70 150 8 /Box 12256071

Bulk SPE Sorbents


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Bondesil® Bulk SorbentsThe same Bondesil sorbent packed into the industry-leading Bond Elut SPE cartridges is available in bulk for large-scale purification or packing custom cartridges. The Bondesil family of bonded silica sorbents is specifically designed for ultra-selective chemical separation such as:

• Custom packing of SPE columns• Flash chromatography• Large-scale preparative separations• HPLC column switching systems• Matrix Solid Phase Dispersion• Low pressure chromatographic systems• Batch extraction from large-volume samples

Bondesil Bulk Sorbents


Size(µm)

Quantity Part No.

2OH 40

10 g 12213029 100 g 12213030

1 kg 12213031 Alumina-N 25 1 kg 12213073

C1 40

10 g 12213002 100 g 12213003

1 kg 12213004

C18

40

10 g 12213011 100 g 12213012

1 kg 12213013

120100 g 14213012

1 kg 14213013 C18 OH 40 100 g 12213049

C2 40

10 g 12213005 100 g 12213006

1 kg 12213007

C8 40

10 g 12213008 100 g 12213009

1 kg 12213010

CBA 40

10 g 12213032 100 g 12213033

1 kg 12213034

CH 40

10 g 12213017 100 g 12213018

1 kg 12213019 CN-E 40 100 g 12213061

CN-U 40

10 g 12213026 100 g 12213027

1 kg 12213028

Certify40 100 g 12213070 120 100 g 14213070

Certify II40 100 g 12213071 120 100 g 14213071

DEA 40

10 g 12213046 100 g 12213047

1 kg 12213048 ENV 125 100 g 12216061

EnvirElut 40

2 g 12212000 100 g 12214016

1 kg 12214019

Bondesil Bulk Sorbents (continued)


Size(µm)

Quantity Part No.

FL 200100 g 12214013

1 kg 12214015 LMS 75 100 g 12216060

NH240

10 g 12213020 100 g 12213021

1 kg 12213022 120 100 g 14213021

PBA 405 g 12213045

10 g 12213044

PH 40

10 g 12213014 100 g 12213015

1 kg 12213016 PPL 125 100 g 12216062

PRS 40

10 g 12213035 100 g 12213036

1 kg 12213037

PSA 40

10 g 12213023 100 g 12213024

1 kg 12213025

SAX 40

10 g 12213041 100 g 12213042

1 kg 12213043

SCX40

10 g 12213038 100 g 12213039

1 kg 12213040 120 100 g 14213039

SI 40 500 g 12213001

SPE Cartridge Processing Stations & Accessories


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CaptiVac™ Vacuum Collars• For use with Captiva Filtration and 96-Deep Well collection plates, and

SPEC SPE 96-well plates.• Prevents cross-contamination• Simple to use• Cost effective

This patent-pending vacuum collar is a completely transparent device that joins Captiva filtration or SPEC SPE 96-well plates directly to our collection plate. The unique design of the CaptiVac collar forms a preset, prealigned vacuum seal between the SPE or filtration device and the collection plate, which positions the outlet tips a specified distance into the collection plate to prevent cross-contamination of samples. The device is so compact, simple, and cost effective that a lab can be outfitted with many of these collars for the cost of a single standard manifold. CaptiVac makes 96-well SPE and filtration simple, practical, and affordable for any size laboratory.iii

CaptiVac Vacuum CollarsDescription Part No. Quantity

CaptiVac Vacuum Collar A796 1CaptiVac Vacuum Collar for Hamilton MICROLAB ATplus 2 A796H 1

CaptiVac Vacuum Collar AccessoriesDescription Part No. Quantity

CaptiVac Gasket Kit A796G 5

Positive Pressure ManifoldPositive pressure is often preferable to vacuum for SPE sample processing. This is because:• Positive pressure provides more than two times greater total force for

moving viscous samples than vacuum• Positive pressure will not dry out SPE cartridges when viscous or other

difficult samples are being processed• Use of nitrogen or other inert gas for positive pressure applications

can improve recovery and extraction reproducibility or easily -oxidized analytes

The Varian Positive Pressure Mani-fold ensures fast and reproducible parallel processing of up to 24 Bond Elut cartridges (1, 3, 6, or 10 mL sizes). Pressure at each cartridge (up to 50 psig) is automatically controlled with individual valves that shut off flow when the cartridge is drained. This prevents faster flowing cartridges from drying out, so there’s no need for any manual intervention, such as shutting off stopcocks, which is typically done with vacuum manifolds. The Varian

Positive Pressure Manifold is constructed of rugged stainless steel and solvent resistant components that provide many years of trouble-free use.iiii

Positive Pressure Manifold


Positive Pressure Manifold with 10 x 75 mm, collection rack, complete system 12234201 1




Both sets of samples generated by precipitating bovine plasma with acetonitrile (1:4, v/v)Centrifugation performed at 3000 rpm for 5 minutes.HPLC gradient: 10% acetonitrile (0.5 min), ramp to 60% acetonitrile (0.5 min), hold for 2 min, ramp to 10% acetonitrile (0.5 min)HPLC column: Polaris C18-A, 3 um, 2 x 50 mm.Injection - 100 uL



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Positive Pressure Manifold Replacement Components and Accessories

iiiiiPositive Pressure Manifold Replacement Components and Accessories

Item # Description Part No. Quantity(Each)

1 Pressure Regulator 12234204 1

2

Collection Rack for 10 x 75 mm, test tubes 12234211 1

Collection Rack for the 12 x 75 mm, test tubes 12234210 1



3 Waste Collection Tub 12234231 1

4 Silicone Rubber Gasket 12234230 1

5

Adapter Plate for 1 mL Bond Elut Cartridge 12234220 1



Adapter Plate for LRC, 10 mL Bond Elut Cartridge 12234223 1

Vac Elut® 20 Manifold

Engineered to increase laboratory productivity, the corrosion-resistant Vac Elut 20 allows simultaneous processing of up to 20 Bond Elut cartridges. The manifold’s clear glass base allows careful monitoring of the entire sample collection process. Its compact, linear design requires very little benchtop space.

• Clear glass base• Disposable needles eliminate cross contamination• Increased productivity/sample throughput• Rugged, reliable construction• Simple vacuum adjustment• Multiple rack options

Rugged constructionOn the Vac Elut 20, the vacuum-control valve, vacuum gauge, and quick-release valve are mounted on the lid, away from the corrosive solvent stream and within convenient reach. The solvent-resistant polypropylene rack is available in a variety of sizes to accommodate the collection tubes commonly used in sample preparation.

Freedom from sample contaminationTo minimize the risk of sample carryover, low-cost, disposable, medical-grade polypropylene delivery needles can be easily replaced. Polypropylene extender tips are also available as a replacement for the standard needle valves, ensuring a direct path into the collection tube. Correct sample identification is also ensured by an interlocking fit between the lid and internal test tube rack.iiii

Vac Elut 20 Manifold


Vac Elut 20 Manifold with Collection Rack for 10 x 75 mm, test tubes 12234105 1







60 SPE Cartridge Processing Stations & Accessories


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Vac Elut® 20 Manifold - Tall Glass Basin

Varian offers a Vac Elut 20 with a large glass basin and collection rack that accommodates the larger 16 x 150 mm test tubes (on the left of the photo). The same high quality material and features on the standard Vac Elut system are incorporated on this special unit. These larger collection vessels can be utilized in combinatorial chemistry applications using large boiling tubes for collection of purified synthesis mixtures, or for any SPE extraction in which an elution volume greater than 10 mL is required.iiii


Vac Elut 20 Manifold with the Tall Glass Basin and Collection Rack for 16 x 150 mm, test tubes, complete system

12234104 1

Collection rack for 10x75 mm Test Tubes 12234517 1

Vac Elut® 20 Manifold Racks and Glass Basinsiiiii

Vac Elut 20 Manifold Racks and Glass Basins

Description Part No. Quantity(Each) Image

Standard Glass Basin 12234505 1

Tall Glass Basin 12234512 1Collection rack for 13 x 75 mm, test tubes

12234507 1

Collection Rack for 13 x 100 mm, test tubes

12234508 1


12234509 1


12234510 1

Vac Elut® 20 Manifold Replacement Components and Accessoriesiiiii

Vac Elut 20 Manifold Replacement Components and Accessories

Description Part No. Quantity ImagePolypropylene Delivery Needles 12234511 25 /Pk

Replacement Exit Valve for Glass Basin

12234506 1 Each

Replacement Lid Gasket 12234502 1 Each

Vac Elut 20 Lid Cover 12234501 1 Each

Vacuum Gauge Assembly 12234504 1 Each

Vac Elut® SPS 24 ManifoldThe Vac Elut SPS 24 allows you to process up to 24 SPE cartridges simultaneously. Like all Vac Elut manifolds, the SPS 24 is made from durable, solvent-resistant materials and engineered to last. The glass sides allow easy viewing of the entire sample collection process.

The highlighting feature of the SPS 24 system is its waste diversion funnel that enables all steps of the SPE procedure to be completed without removing the lid. The collection rack can be placed inside the unit before the extraction begins. This unique characteristic of the SPS 24 eliminates splash-back, prevents cross contamination, and minimizes hazardous waste, and biohazard exposure.

Complete with replacement stainless steel delivery tips for maximum extract purity, the Vac Elut SPS 24 system also includes a vacuum controller/release, collection rack, and port sealing plugs. Racks for several different collection tube configurations are available.iiii

Vac Elut SPS 24 Manifold


Vac Elut SPS 24 Manifold with Collection Rack for 10 x 75 mm, test tubes 12234003 1






62

Vac Elut® SPS 24 Replacement Componentsiiiii

Vac Elut SPS 24 Replacement ComponentsDescription Part No. Quantity

Collection Rack and Funnel Set for 10 x 75 mm, test tubes 12234026 1 Each

Collection Rack and Funnel Set for 12 or 15 mL Conical Tubes 12234027 1 Each




Elastic Lid Fasteners - 6/pk 12234034 6 /Pk

Lower Lid Seals - 2/pk 12234050 2 /Pk

SPS 24 Glass Basin 12234024 1 Each

SPS 24 Lid Cover 12234025 1 Each

SPS 24 Spare Parts Kit 12234033 1 EachSPS 24 Waste Tower Repair Kit, Base Exit Tube, Hose Connector, Washer, Center Tube, 90º Connector Elbow

12234005 1 Each

Solvent Resistant Vacuum Gauge 12234023 1 Each

Stainless Steel Delivery Needles - 25/pk 12234038 25 /Pk

Upper Lid Seals - 2/pk 12234051 2 /Pk

Vac Elut SPS 24 Lid Screw Kit - 12/pk 12234021 12 /Pk

Waste Funnel for 10 x 75 mm, test tubes 12234035 5 /PkWaste Funnel for 12 x 75 or 13 x 100 mm, test tubes - 5/pk 12234032 5 /Pk

Waste Funnel for 16 x 100 mm, test tubes 12234029 5 /Pk

Welch Vacuum Chemical Duty Vacuum Pumps• PTFE chemical duty pumps• Free air displacement from 22 to 45 L/min• Exceed performance of water aspirator• Recommend 19/22 L/min for VacElut 20, SPS 24 • Recommend 38/45 L/min for VersaPlate, EnvirElut

Welch Vacuum Chemical Duty Vacuum PumpsVoltage Description Part No. Quantity

115V/60Hz

22L/min at 26.7”Hg: 115V/60Hz WL2012B01 1

45L/min at 26.7”Hg: 115V/60Hz WL2015B01 1

230V/50Hz

19L/min at 26.7”Hg: 230V/50Hz WL2012C02 1

38L/min at 26.7”Hg: 230V/50Hz WL2015C02 1

Solid Phase Extraction Publications


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Handbook of Sorbent Extraction Technology

Varian offers the internationally acclaimed Handbook of Sorbent Extraction Technology. This handbook, now in its third edition, is the most comprehensive guide to method development available, containing easy-to-follow information on:• logical, efficient method development• troubleshooting tips for improving analyte recovery and method

reproducibility• technical discussions on the nature of bonded silica sorbents• practical examples of SPE applications using all the commonly

encountered mechanisms: non-polar, polar, ion exchange, covalent, and mixed-mode

• effective clean up of difficult samples such as foodstuff, soil or tissues• Indices of functional group pKas, counter-ion selectivities, and much

more.

The Handbook of Sorbent Extraction Technology has proven to be an invaluable reference source for chemists worldwide. Over 15,000 copies have been sold globally.

Item Name Item Code Quantity(Each)

Handbook of Sorbent Extraction Technology 12245005 1

Solid Phase Extraction: Principles, Techniques, and Applications

If you require a complete reference resource of Solid Phase Extraction technology, then you will want to get the new textbook published by Marcel Dekker Inc. and offered by Varian. It is the only comprehensive textbook that covers the broad field of SPE. No one person is expert in all aspects of the application of SPE. Therefore this book contains submissions from a variety of independent experts in each field. Packed with over 500 pages of expertise, it contains a complete index, over a thousand references and several hundred diagrams and chromatograms.

Description Part No. QuantitySolid-Phase Extraction, Principles, Techniques, and Applications PUBSPE001 1

Solid Phase Microextraction

This comprehensive reference contains extensive descriptions of proven sampling methods for chemical analyses. The book focuses on applications of solid phase microextraction (SPME) for laboratories analyzing dirty sample matrices and requiring high sample throughput, quick sample turnaround time, and low detection levels. Provides a sound theoretical basis for SPME, plus highly detailed descriptions of pharmaceutical, environmental, food science, forensic, and toxicological applications.

Description Part No. QuantitySolid Phase Microextraction PUB0824770587 1



64 Solid Phase Microextraction


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SPME Fibers

SPME fiber assemblies can be reused for up to 100 analyses or more, depending on the application and the care they are given. For reuse, simply condition with solvent or heat before and after every analysis. Each assembly has a color-coded or notched hub indicating the type of coating on the fiber. Choose the assembly that is appropriate for the holder: manual, or Varian AutoSampler (also used for HPLC applications). First time SPME users must order both a holder and a fiber assembly.

Recommendations:For gases and low molecular weight compounds (MW from 30 to 225): - Carboxen/PDMS fibers

For volatiles (MW from 60 to 275): - 60 µm PDMS fibers

For semivolatiles (MW from 80 to 300): - polyacrylate fibers

For nonpolar high molecular weight compounds (MW from 125 to 600): - 7µ PDMS fibers

For nonpolar semivolatiles (MW from 80 to 500): - 30 µm PDMS fibers

For alcohols and polar compounds (MW from 40 to 275): - Carbowax/DVB fibers

For flavors (MW from 40 to 275): - Carboxen/DVB/PDMS on a StableFlex fiber

For trace levels (MW from 40 to 275): - Carboxen/DVB/PDMS on a 2 cm StableFlex fiber

SPME Kits offer a convenient assortment of fibers for method development and first-time sample analysis.

SPME Fibers3 per pack

Inlet Usage Coating Length(cm)

Fiber OD(µm) Fused Silica StableFlex Gauge

Septum

A/S

Carbowax/DVB 165 391896312 2470 SU57337U 24

Carbowax/PDMS 1 85 SU57335U 24Carboxen/DVB/PDMS 1 50/30 SU57329U 24

Carboxen/PDMS 1 75 391896316 24

PDMS 17 391896303 2430 391896310 24100 391896302 24

PDMS/DVB 1 65 391896314 SU57327U 24Polyacrylate 1 85 391896306 24

Manual

Carbowax/DVB 1 65 391896311 24

Carboxen/DVB/PDMS1 50/30 SU57328U 242 80 SU57348U 24

Carboxen/PDMS 1 75 391896315 24

PDMS 17 391896304 2430 391896309 24100 391896301 24

PDMS/DVB 1 65 391896313 SU57326U 24Polyacrylate 1 85 391896305 24

Merlin Microseal

A/S

Carbowax/DVB 1 70 SU57339U 23Carboxen/PDMS 1 75 SU57343U 23

PDMS 1 100 SU57341U 23PDMS/DVB 1 65 SU57345U 23

Manual

Carbowax/DVB 1 70 SU57338U 23Carboxen/PDMS 1 75 SU57344U 23

PDMS 1 100 SU57342U 23PDMS/DVB 1 65 SU57346U 23

DVB = divinylbenzene. PDMS = polydimethylsiloxane



64 Solid Phase Microextraction


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SPME Kits

Inlet Usage Item Name Part No. Quantity(/Pk)

Fiber OD(µm)

Length(cm) Gauge

Septum

A/S

Kit 1, PDMS, 85 µm, 100 µm, 7 µm, Automated Sampling 391896308 3 7, 85, 100 1 24

Kit 2, Carbowax/DVB, PDMS/DVB, Automated Sampling SU57321U 3 65, 75 1 24

Kit 3 for HPLC, Carbowax/TPR, PDMS/DVB, Automated Sampling SU57323U 3 50, 60, 100 1 24

Kit 4 PDMS, PDMS/DVB, PDMS/ Carboxen, Automated Sampling SU57325U 3 50, 65, 75 1 24

StableFlex Fiber Assortment Kit, Automated Sampling SU57551U 4 50/30, 65,70, 85 1 & 2 24

Manual

Kit 1, PDMS, 85 µm, 100 µm, 7 µm, Manual Sampling 391896307 3 7, 85, 100 1 24

Kit 2, Carbowax/DVB, PDMS/DVB, Manual Sampling SU57320U 3 65, 75 1 24

Kit 4, PDMS, PDMS/DVB, PDMS/Carboxen, Manual Sampling SU57324U 3 50, 65, 75 1 24

StableFlex Fiber Assortment Kit, Manual Sampling SU57550U 4 50/30, 65,70, 85 1 & 2 24

SPME Accessories


Merlin Microseal Replacement for SPME, 23GA 392609902 1

Merlin Microseal SPME Kit for 1079 Injector, 23 GA 392609901 1 Kit

Merlin Microseal SPME Kit for 1177 Injector, 23 GA 392609903 1 Kit

SPME 15 mL Stand SU57357U 1

SPME Fiber Holder for CTC Autosampler SU57347U 1

SPME Fiber Holder for Manual Sampling 391896401 1

SPME Fiber Holder for Varian Autosampler, or HPLC analysis SU57331 1

SPME Inlet Guide for Manual Injection. Fits most Varian and Agilent injectors. SU57356U 1

SPME Link Septa, 11 mm 392548402 25 /Pk

SPME Sensitivity Test Sample 391896700 5 /Pk


This comprehensive reference contains extensive descriptions of proven sampling methods for chemical analyses. The book focuses on applications of solid phase microextraction (SPME) for laboratories analyzing dirty sample matrices and requiring high sample throughput, quick sample turnaround time, and low detection levels. Provides a sound theoretical basis for SPME, plus highly detailed descriptions of pharmaceutical, environmental, food science, forensic, and toxicological applications.

Description Part No. QuantitySolid Phase Microextraction PUB0824770587 1

Documents

2-Varian 2005 Sample Prep Web