Nhm thc hin: Nhm 01GVHD: Th.s Trn Nguyn An Sa

Ni dungTng quan k thut ng chunng dng xc nh hm lng Fe(II) v tng Fe Nguyn tcCc cht gy nhiuHo chtDng c - thit bCch tin hnh

K thut ng chun

u im- Vi mt ng chun cho php phn tch hng lot mu nn nhanh v hiu qu kinh t.2.Nhc imCn c my, my cng chnh xc th kt qu cng tin cy.S hp thu nh sng ca dung dch phi tun theo nh lut Bughe- Lambe- Beer.Khng loi c nh hng ca nn mu.u v nhc im

ng dng xc nh hm lng Fe(II) v tng Fe

St l nguyn t vi lng cn thit cho c th. V th, st vi hmlng 0,3 mg/l l mc cho php i vi nc sinh hot. Vt qu giihn trn, st c th gy ra nhng tr ngi:Mi tanh c trng ca st, lm nc c mu to n tng khng tt cho ngi s dng. Nc c st khng th dng cho mt s nghnh cng nghip nh: giy, si, dt, thc phm, dc phm Kt ta st lng ng thu hp dn tit din hu dng ca ng dn trong mng li phn phi nc.

Do cc l do trn, vic xc nh hm lng st trong nc c nghaquan trng trong i sng cng nh trong sn xut cng nghip.

Fe2+ to phc bn c mu cam vi 1,10 phenanthroline.Phc [Fe(Phen)3]2+ bn c max 508nm, cng mu khng thay i trong khong pH t 2 9.

Tng hm lng St: dng hidroxylamine kh Fe3+ v Fe2+.Hm lng Fe3+ = tng hm lng Fe - hm lng Fe2+

Nguyn tc

Nhng cht oxi ha mnh: CN-, NO2-, PO43- Zinc vi hm lng ln hn st 10 lnCopper ln hn 5ppmNiken ln hn 2ppmBitmut,thy ngn, molybdate, bc kt ta vi phenanthroline (thm mt lng tha phenanthroline)Cht hu c (un si mu nhiu gi vi HCl 1:1trong cc s, khi mu cn, t nh, phn tro cn li ha tan bng axit).Cc cht gy cn nhiu

Ho cht

4. Dung dch Fe3+ 15ppm

5. Dung dch NH2OH 10% pha trong nc.6. m axetat pH = 5.7. Dung dch 1,10 phenanthroline 0.5% pha trong nc.

Ho cht

Bnh nh mc 1L01Pipet vch 10ml 01Cuvet 01My pH k01Cn phn tch01Bp cao su01Beaker 250ml03Beaker 100ml02My khuy t01a thy tinh01Pipet bu 5ml02C t01My so mu01

Dng c - thit bMy quang ph Genesye 10UV scanning

Cch tin hnhQut ph v xy dng ng chun.Hiu sut thu hi ca phng php.Kim tra mu th.Vn hnh thit b.

Qut ph v xy dng ng chun

Bnh s123456VFe 50ppm (ml)01.02.03.04.05.0Hydroxylamin (ml)2.0Dung dch m pH = 5 (ml)10.01,10- phenanthroline2.0nh mc bng nc ct n vch 50ml

Qut ph v xy dng ng chun

Hiu sut thu hi ca phng php

Bnh s1234567V_dd B101010V_ dd C101010Hydroxylamin2.00.02.0 yn 10 phtDung dch m pH = 5 (ml)10.01,10- phenanthroline (ml)2.0nh mc bng nc ct n vch 50ml

Kim tra mu

Bnh s1234567V_ddA (ml)101010101010Hydroxylamin (ml)2.00.02.0 yn 10 phtDung dch m pH = 5 (ml)10.01,10- phenanthroline (ml)2.0nh mc bng nc ct n vch 50ml

Vn hnh my

Trng hp my khng kt ni vi phn mm Visionlite

Nhn cng tc ngun ON/OFF trn my, ch n nh.Nhn phm Test mn hnh hin th:

- Advanced A; %T; C- Kinetics- Standard curve- Scanning- Absorbance Ratio- 3-point Net- Absorbance Difference- Mulatiwavelength- Performance ValidationVn hnh my

Nu qut bc sng, nhn phm hoc chn Scanning; sau Enter. Chn khong bc sng cn qut v chn lnh qut bc sng.- Chy nn bng cch qut vi mu trng.- Chy mu bng cch chn mt mu bt k qut.Nu o mu ring bit nhn phm hoc chn ch o Advanced A ; %T; C.Nu xy dng ng chun ta nhn phm hoc chn Standard curve.Vn hnh my

2. Trng hp my o quang kt ni vi phn mm VisionliteNhn cng tc ngun ON/OFF trn my, ch n nh.Khi ng my vi tnh, m phn mm Visionlite

Nu qut bc sng ta chn ch Scan v thc hin cc bc sau:

Vn hnh my

Phn mm gm 4 module: Scan: Ghi nhn ph. Rate:Fixed: Ghi nhn s hp th ca mu ti mt im ca mt bc sng.Quant: nh lng theo nh lut Lambert Beer.

Scan.mscRate.mraFixed.mfxQuant.mqa

Vn hnh my

Scan

Rate

Fixed

Quant

Vn hnh my Nhp cc thng s ca ch qut

- Qut mu trng bng nc ct hoc bnh Blank, ta cho mutrng vo my quang, nhn Baseline hoc Measure samples v sau check vo mc Measure blank v nhnMeasure qut mu trng.

- Sau khi qut mu trng (my bo 100%), ta t mu tht vov nhn Measure qut mu tht, v lu file vi ui msc.Nu xy dng ng chun, ta chn ch Quant, v thc hin cc bc sau:

Vn hnh my

Vn hnh my - Nhp cc thng s xy dng ng chun

Sau khi nhp cc thng s xy dng ng chun, ta nhn Measure standards v sau check vo mc Measure blank v nhn Measure o mu trng. Sau khi o mu trng (my bo 100%) , ta ln lt t cc chun t thp n cao v nhn Measure o chun, lu file vi ui mqa. o mu, ta m li file ng chun lu, t mu trng v nhn Measure sample v sau check vo mc Measure blank v nhn Measure o mu trng. Sau khi o mu trng (my bo 100%), ta ln lt t mu tht, nhp thng tin mu, nhn Measure o mu tht.Vn hnh my

Kt lunNm r kin thc v k thut ng chun.ng dng xc nh hm lng Fe2+ v Fe tng.Cch kho st bc sng cc i, xy dng ng chun.Vn hnh my o quang ph Genesye 10UV scanning v phn mm Visionlite.

Ti liu tham khoGio trnh thc hnh phn tch ha l.Trn T Hiu(2003), Phn tch trc quang ( Ph hp th UV Vis), NXB i hc quc gia H Ni, H Ni.Http://www.google.com.vn/searchHttp://www. Bioblock.com