196

amino acids, and culturing is continued into the decline phase of the culture to provide the pro- duct(s) of the cells. The process is particularly applicable to genetically modified cells, espe- cially hybridoma cell cultures to produce mono- clonal antibodies. Preferably, the supplemental feed is fed to the culture at a slow rate over a pro- longed period. Very significant enhancement of overall product yield may be obtained.

8700196

P R O T E C T I O N O F P R O T E I N S A N D T H E L I K E

Bruce Joseph ROSER, The Old Vicarage, Church Lane, Balsham, Cambridgeshire, United Kingdom assigned to QUADRANT BIO- RESOURCES LIMITED

Sensitive proteins and other macromolecules, such as enzymes, antibodies, antigens, serum complement, fluorescent proteins, vaccine com- ponents, polysaccharides such as agarose etc, can be preserved by drying at ambient tempera- ture and at atmospheric pressure in the presence of trehalose. A porous matrix impregnated with trehalose is provided as a receiver for a blood or other liquid sample to be dried.

8700201

E P I T H E L I A L C E L L S E X P R E S S I N G F O R E I G N G E N E T I C M A T E R I A L

Jeffrey R MORGAN, Richard C MULLIGAN assigned to WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH

The foreign genetic material can be DNA or RNA which does not occur in epithelial cells; DNA or RNA which occurs in epithelial cells but is not expressed in them at levels which are bio- logically significant; DNA or RNA which occurs in epithelial and has been modified so that it is expressed in epithelial cells; and any DNA or RNA which can be modified to be expressed in epithelial cells, alone or in any combination thereof. In addition, epithelial cells can express genetic material encoding a selectable marker by which cells expressing the foreign genetic material can be expressed.

PATENT ABSTRACTS

8700436

T H E P R O T E C T I O N O F E Q U I N E S A G A I N S T S T R E P T O C O C C U S

E Q U I

John F TIMONEY. John F TIMONEY as- signed to CORNELL RESEARCH FOUNDA- TION INC;

A new bacterial vaccine to protect susceptible equine against S. equi which causes strangles. The vaccine stimulates a nasopharyngeal im- mune response in a susceptible equine through the presence of antibody activity in the nasopharyngeal mucus. The vaccine is a S. equi strain which contains an M protein fragment of 41,000 mw and is adapted for administration to equine either intranasally or orally as a vaccine. There is described a new strain of S. equi (709- 27), a method of making and isolating useful vaccine strain of S. equi bacteria which stimulates an antibody response in the nasopharyngeal mucosa of the susceptible equine.

8700526

I M M U N O C H E M I C A L A S S A Y S F O R H U M A N A M Y L A S E

I S O E N Z Y M E S A N D R E L A T E D M O N O C L O N A L A N T I B O D I E S ,

H Y B R I D O M A C E L L L I N E S A N D P R O D U C T I O N T H E R E O F

David E BRUNS, David C BENJAMIN as- signed to THE UNIVERSITY OF VIRGINIA ALUMNI PATENTS FOUNDATI

Immunoehemical assays for human amylase isoenzymes. These assays utilize monoclonal antibodies which are produced from hybridoma cell lines and are specific for human salivary type amylase but not human pancreatic amylase. The assays involve reacting this monoclonal anti- body with the unknown sample for the purpose of studying quantitatively and qualitatively the amylase isoenzymes present. The results from such assays are useful in the diagnosis of disease or ruling out disease. Antibody producing hybridoma cell lines and their production.

8700531

M O N O C L O N A L A N T I B O D I E S A N D T H E I R U S E

Bruce William WRIGHT. Peter John COX. Alice Margaret NOYES, Danny WIDDOWS, 48 Bush Close, Comberton, Cambridgeshire CB3 7EG. United Kingdom assigned to TECH- NOLOGY LICENCE COMPANY LIMITED