YeastYeast 2013; 30: 291.Published online 19 June 2013 in Wiley Online Library(wileyonlinelibrary.com) DOI: 10.1002/yea.2963

Erratum

A new versatile system for rapid control of geneexpression in the fission yeast Schizosaccharomyces pombeNicola Zilio*, Sophie Wehrkamp-Richter and Michael Nicholas BoddyDepartment of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, USA

Yeast 29: 425-434Article first published online: 12 SEP 2012DOI: 10.1002/yea.2920

In the version of this article initially published, the reverse primer sequence for pFA6a-tetO7-TATACYC1(No Tag) was mistakenly reported as 5’-(gene-specific sequence)-CATGATTTAACAAAGCGACTATA-3’. It should instead be 5’-(gene-specific sequence)-CCAGGATGATAAACGGATCC-3’ (see correctedFigure 1).It has also come to our attention that many transcription activation domains, including that of the her-

pes simplex virus protein VP16, are toxic in fission yeast (doi:10.1093/emboj/16.18.5722), which mayexplain why it is not possible to obtain stable integrants of the pUG6SP-(tTA/tTA’) vectors. Unfortu-nately, this issue is probably a generic effect of the highly acidic nature of these domains and it is unlikelybe a solvable in fission yeast. This limitation does not undermine the promoter system we developed andall other conclusions still hold.

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