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Plant Cell Lysis
www.piercenet.com/pper38j
The P-PER® Reagent Kit † (#89803) extractsmaximum active protein from stem, root, seed andleaves in 10 minutes without liquid nitrogen.
You can trust the experts at Pierce to know how to get max-imum protein extraction from your sample. We have beenthe protein people for over 50 years, providing the bestsolutions to extract, purify and quantify proteins from manytypes of cell lines and tissue.
Is your plant cell lysis leafing you out in the cold?
Fresh leaf tissue and seed were lysed and extracted according to the P-PER® Kit (Product # 89803)protocol, a competitor’s protocol and a literature-based (home brew) protocol. Samples were normalized(weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with Imperial™ ProteinStain† (Product # 24615). Samples were also quantified using the BCA™ Protein Assay Kit, ReducingAgent Compatible (Product # 23250).
*The Competitor S kit is not recommended for dried seed.
Tel: 815-968-0747 or 800-874-3723 • Fax: 815-968-7316 • Customer Assistance E-mail: [email protected] the United States, visit our web site or call 815-968-0747 to locate your local Perbio Science branch office (below) or distributor
© Pierce Biotechnology, Inc., 2005. Pierce products are supplied for laboratory or manufacturing applications only.
BCA™, P-PER® and Imperial™ are trademarks of Pierce Biotechnology, Inc. † U.S. patents pending on P-PER® Technology, Imperial™ Protein Stain and Reducing Agent-compatible BCA™ Technology.
Belgium & Dist.:Tel +32 53 85 7184
China:Tel +86 10 8049 [email protected]
France:Tel 0800 50 82 15
Germany: Tel 0228 9125650
Hong Kong:Tel 852 2753 0686
The Netherlands: Tel 076 50 31 880
United Kingdom: Tel 0800 252185
Switzerland: Tel 0800 56 31 40
P-PER® Reagent Kit Highlights:
• Convenient – disrupts cells without harsh mechanical methods in 10 minutes
• Compatible – downstream applications include 1-D and 2-D gel electrophoresis,Western blotting, activity assays, and protein affinity purifications
• Quantifiable – use the BCA™ Protein Assay Kit, Reducing Agent Compatible†
• Ready-to-use – extract does not require filtration through cheesecloth or Miracloth
• Provides active proteins – extracted proteins are functional
New! Poppers™ Cell Lysis Solutions HandbookLog on to our web site or call us to requestyour FREE handbook (#1601234). Outsidethe U.S., contact Perbio Science or your local distributor.
200
976643
29
2014
6
3.6
0.02 0.016 0.009
Leaf
MW (kD)
Protein extracted (mg)/ total tissue weight (mg)
Pie
rce
Tobacco
Hom
e B
rew
Com
pet
itor
S
0.02 0.02 0.01
Pie
rce
Hom
e B
rew
Com
pet
itor
S
0.176 0.09
Pie
rce
Hom
e B
rew
0.056 0.046
Pie
rce
Hom
e B
rew
0.09 0.058 0.063
Pie
rce
Hom
e B
rew
Com
pet
itor
S
Corn Leaves Arabidopsis Soybean Corn Kernel
Seed*
G r a s p t h e P r o t e o m e ®
eTOCsThe Plant Cell's FREE eTOC service allows anyonewho registers to be notified via e-mail when newcontent goes online. You may choose to receiveany or all of the following:• Notification that a new issue of The Plant Cell
is online• Planned tables of contents for future issues• Complete table of contents for new issues• Special Announcements from ASPB
SIGN UP NOW!!!http://www.plantcell.org/cgi/alerts/etoc
The Plant Cell (www.plantcell.org) and Plant Physiology(www.plantphysiol.org), ASPB’s premier plant science journals,
now allow you to save any figure as a PowerPoint slide! This
free feature is available for all articles published since 1998.
From the full-text (non-PDF) version of an article, click to
“View larger version” of a figure. Then click the button marked
“PowerPoint Slide for Teaching.”The slide will include the full
bibliographic citation of the article in which the figure was
published.
We hope that you enjoy this new feature and that itenhances the teaching of plant biology in your classroom.
as PowerPoint Slides!
Download Figuresfrom The Plant Cell and Plant Physiology
To purchase the images from Biochemistry & Molecular Biology ofPlants using our secure web site, go to http://www.aspb.org/
publications/biotext/imagelibrary/. Log in as a member for your
member discount. Contact [email protected] for more information.
ASPB announces the new Online Image Library – containing
all images from the best-selling textbook/reference work
Biochemistry & Molecular Biology of Plants, by Buchanan,
Gruissem, and Jones.
The new Online Image Library features images listed by chapter plus the capabili-
ty to search by individual images. And images are easily imported into
PowerPoint for use in presentations.
Access to the site is available for $49.95. ASPB members receive a 20% DISCOUNT,
making the purchase price for members $39.95.
Biochemistry & Molecular Biology of Plants
Online Image Library!ADVERTISING IN THE PLANT CELL
For information about display and classified advertisements in
The Plant Cell, contact:
Kelly Taylor
Leonard Media Group
P.O. Box 220
415 Horsham Road
Horsham, PA 19044 USA
Telephone: 215-675-9133, ext. 226
Fax: 215-675-9376
Email: [email protected]
Or visit our website at:
www.plantcell.org/misc/pcadv.shtml
The Open Access movement in scholarly publishing advocates that research content shouldbe freely available to all immediately upon publication. This approach has prompted publish-ers to examine the feasibility of a shift from traditional subscription-based (“user pays”)financial models to an “author-pays” model, in which some or all of the costs of publicationare typically borne by authors.
What does our author community think about Open Access? To gauge the plant sciencecommunity’s interest in this new approach to publishing and to help ASPB determine theviability of “author-pays” publishing models, the Society is conducting an 18-month OpenAccess “experiment.” Beginning with the December 2005 issues of The Plant Cell and PlantPhysiology, authors of articles accepted by the journals will be given the option to pay a sur-charge to make their online article free from the moment of publication to anyone withInternet access. The surcharge, which is in addition to the usual author charges, will be$1,000 (discounted to $500 if the author’s institution subscribes to the journal).
For more information, go to http://www.aspb.org/publications/openaccess.cfm or contactNancy Winchester, ASPB director of publications, at [email protected].
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Joint Annual Meeting of the American Society of Plant Biologists
and the Canadian Society of Plant Physiologists
Société Canadienne de Physiologie Végétale
Hynes Convention Center
Boston, MassachusettsAugust 5-9, 2006
For more information: American Society of Plant BiologistsTelephone: 301-251-0560 • Fax: 301-279-2996 • E-Mail: [email protected] • Web Site: http://www.aspb.org/meetings/pb-2006
Photo Credit: Greater Boston Convention & Visitors Bureau
SYMPOSIAPlants Mitigating Global Change—Stephen P. Long, University of Illinois
Legumes: Genomes to Biology—Douglas R. Cook, University of California-Davis
Ion Channels and Cellular Signaling—Julian I. Schroeder, University of California-San Diego
Gibbs Medal Symposium: Genome Scale BiologyJoseph R. Ecker, The Salk Institute
President's Symposium: Plant Responses to the EnvironmentMichael F. Thomashow, Michigan State University
Plan
t Bio
logy