AMT AND ITS METABOLITES 251 than NT level. In the urine, AMT, NT, 10-OH-AMT, 10-OH-NT are excreted in the range of 0-1, 1-2, 2-6 and 10-50 mg/g creatinine respectively. The ratio of 10-OH-NT/NT in the urine ranged 10-30 for pa- tients with normal liver function. Our observations about correlation between serum and urinary levels of AMT and metabolites and control of depression will be published elsewhere. ACKNOWLEDGEMENTS For financial support to the Medical Research Council, Grant No. MA-5563 and St. Joseph's Hospital Foundation, Grant No. 820-042. For advice and research samples to Albert Manian, N.I.M.H. and Merck Research Institute. For help in the collection and handling of blood sam- pies and collection of patient data to Lois Barry. For secretarial assistance to Anne Robitaille. REFERENCES ]. Oliver, J. S. and Smith, H. (1974). For. Sc. 3:181-187. 2 Hamilton, H. E., Wallace, J. E. and Blum, K. (1975). A~al. Chem. 47:1139-1143. 3. Faber, D. B., Mulder, C. and Man In'T Veld, W. A. (1974). J. Chromatogr. 100:55-61. 4. Braithwaite, R. A. and Widdop, B. (1971). Clim Chim. Acta, 35:461-472. 5. Hucker, H. B. and Stauffer, S. C. (1974). J. PhcIrm. Sc. 63:296-297. 6. Norheim, G. (1974). J. Chromatogr. 88:403-406. 7. Eichholtz, P. C. N. (1975). J. Chromatogr. 111:456- 458. 8. Jorgensen, A. (1975). Acta Pharmacol. et Toxicol. 36:79-90. 9. Reite, S., Salvesen, B. and Skaug, O. (1975). Medd. Norsk. Far~. Selsk. 37:55-63. 10. Hartvig, P., Strandberg, S. and Nausland, B. (1976). J. Chromatogr. 118:65-74. 11. Biggs, J. T., Holland, W. H., Chang, S., Hipps, P. P. and Sherman, W. R. (1976). J. Pharm. So. 65:261-268. 12. Gard, H., Knapp, D., Walle, T. and Gaffney, T. (1973). Clh~. Toxicol. 6:571-584. ]3. Usdin, E. and Efron, D. H. (1972). Psychotropic Drugs and Related Compounds, National Institute of Mental Health, Rockville, Md. 14. Gram, L. F. (1974). Dan. Med. B~dl. 21:218-231. 15. Judd, C. I. and Ursillo, R. C. (1975). In Industrial Pharmacology Vol. 2 (Fielding, S. and Lal, H., ed.), Futura, New York, 231-265. 16. Borga, 0. and Garle, M. (1972). J. Chromatogr. 68: 77-88. 17. Toseland, P. A., Albani, M. and Gauchel, F. D. (1975). Cli~. Chem. 21:(98-103). -k ~ "k LETTER TO THE EDITOR Bilirubin Quantitation with Lipemic Plasma Clin. Biochem. 9, 2, 96-98 (1976) It has been brought to our attention that while the AO bilirubinometer was rather insensitive to Intralipid lipemia in bilirubin estimation in artificially jaundiced human serum albumin, this principle does not necessarily apply to jaundiced plasma. The effects of lipemia on bilirubin estimation by the AO bilirubinometer were therefore determined in four different adult plasma samples (Tri- glyceride El-G) less than 20 mg/dl, Bilirubin less than 0.5 mg/dl). As with Human Serum Albumin, TG levels of 50 mg//dl or less caused no interference in bilirubin estimations. At higher concentrations of Intralipid (100- 750 mg/dl), there is a linear increase of l mg/dl of bilirubin per 100 mg/dl (see figure l) and this effect seems to be independent of the bilirubin concentration used in the experiments (2.5 to 25 mg/dl). Therefore, if the TG concentrations in the samples are known a subtraction of 1 mg/dl bilirubin per 100 mg/dl of lipid will give the actual bilirul~in concentration. George Chan, Kathy Merrills and David Schiff, Department of Pediatrics University of Alberta Edmonton, Alberta.

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Page 1: Bilirubin quantitation with lipemic plasma

AMT AND ITS METABOLITES 251

than NT level. In the urine, AMT, NT, 10-OH-AMT, 10-OH-NT are excreted in the range of 0-1, 1-2, 2-6 and 10-50 m g / g c rea t in ine respectively. The rat io of 1 0 - O H - N T / N T in the ur ine ranged 10-30 for pa- t ients with normal liver funct ion. Our observat ions about correlat ion between serum and u r i n a r y levels of AMT and metaboli tes and control of depression will be published elsewhere.

ACKNOWLEDGEMENTS

For f inanc ia l support to the Medical Research Council, G ran t No. MA-5563 and St. Joseph's Hospital Foundat ion, Gran t No. 820-042.

For advice and research samples to Alber t Manian, N.I.M.H. and Merck Research Ins t i tu te .

For help in the collection and handl ing of blood sam- pies and collection of pa t ient data to Lois Barry.

For secretar ia l assis tance to Anne Robitaille.

REFERENCES

]. Oliver, J. S. and Smith, H. (1974). For. Sc. 3:181-187. 2 Hamilton, H. E., Wallace, J. E. and Blum, K. (1975).

A~al. Chem. 47:1139-1143. 3. Faber, D. B., Mulder, C. and Man In 'T Veld, W. A.

(1974). J. Chromatogr. 100:55-61. 4. Braithwaite, R. A. and Widdop, B. (1971). Clim

Chim. Acta, 35:461-472.

5. Hucker, H. B. and Stauffer, S. C. (1974). J. PhcIrm. Sc. 63:296-297.

6. Norheim, G. (1974). J. Chromatogr. 88:403-406. 7. Eichholtz, P. C. N. (1975). J. Chromatogr. 111:456-

458. 8. Jorgensen, A. (1975). Acta Pharmacol. et Toxicol.

36:79-90. 9. Reite, S., Salvesen, B. and Skaug, O. (1975). Medd.

Norsk. Far~. Selsk. 37:55-63. 10. Hartvig, P., Strandberg, S. and Nausland, B. (1976).

J. Chromatogr. 118:65-74. 11. Biggs, J. T., Holland, W. H., Chang, S., Hipps, P. P.

and Sherman, W. R. (1976). J. Pharm. So. 65:261-268. 12. Gard, H., Knapp, D., Walle, T. and Gaffney, T.

(1973). Clh~. Toxicol. 6:571-584. ]3. Usdin, E. and Efron, D. H. (1972). Psychotropic

Drugs and Related Compounds, National Institute of Mental Health, Rockville, Md.

14. Gram, L. F. (1974). Dan. Med. B~dl. 21:218-231. 15. Judd, C. I. and Ursillo, R. C. (1975). In Industrial

Pharmacology Vol. 2 (Fielding, S. and Lal, H., ed.), Futura, New York, 231-265.

16. Borga, 0. and Garle, M. (1972). J. Chromatogr. 68: 77-88.

17. Toseland, P. A., Albani, M. and Gauchel, F. D. (1975). Cli~. Chem. 21:(98-103).

-k ~ "k

LETTER TO THE EDITOR

Bilirubin Quantitation with Lipemic Plasma

Clin. Biochem. 9, 2, 96-98 (1976)

It has been brought to our attention that while the AO bilirubinometer was rather insensitive to Intralipid lipemia in bilirubin estimation in artificially jaundiced human serum albumin, this principle does not necessarily apply to jaundiced plasma. The effects of lipemia on bilirubin estimation by the AO bilirubinometer were therefore determined in four different adult plasma samples (Tri- glyceride El-G) less than 20 mg/dl, Bilirubin less than 0.5 mg/dl) . As with Human Serum Albumin, TG levels of 50 mg / /d l or less caused no interference in bilirubin estimations. At higher concentrations of Intralipid (100- 750 mg/dl) , there is a linear increase of l mg/dl of bilirubin per 100 mg/dl (see figure l) and this effect seems to be independent of the bilirubin concentration used in the experiments (2.5 to 25 mg/dl) . Therefore, if the TG concentrations in the samples are known a subtraction of 1 mg/dl bilirubin per 100 mg/dl of lipid will give the actual bilirul~in concentration.

George Chan, Kathy Merrills and David Schiff, Department of Pediatrics

University of Alberta Edmonton, Alberta.