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Cellular Respiration
Biology 1406
Objectives: Cellular Respiration
1. Define the following terms as they relate to cellular
respiration:Aerobic respiration- when cells in an environment w/
oxygen are capable of
metabolizing glucose NAD+- electron carrier, func. as an
oxidizing agent during
respiration Lactic acid- created during fermentation, human
muscle cells make when
oxygen is scarce
Anaerobic respiration - processed used to harvest chemical
energy w/out oxygen
NADH- reduced form of NAD+, by electrons released from the
oxidation of glucose, still
has energy in it Alcohol- released by fermentation, pyruvate is
converted into ethanol intwo steps.
Cellular respiration- oxygen is consumed as a reactant to help
make chemical energy(can include both aerobic and anaerobic)
FAD
+-oxidized form of FADH2
Oxidation-loss of electrons
Glycolysis-occurs in the cytosol, breaks glucose down into two
molecules of pyruvate
FADH2-another electron carrier reduced form of FAD+Reduction-
gain of electrons
Fermentation- when electron transport chain and oxygen arent
present in the cell
ATP-main energy molecule produced during cellular
respiration
Krebs cycle- a.k.a citric acid cycle, takes place in the matrix,
oxidizes a derivative of
pyruvate into carbon dioxide Mitochondria- Where cellular
respiration takes placeElectron Transport Chain-collection of
molecules embedded in the inner membrane
of mitochondrian that help transport electrons to ATP synthase
Pyruvic acid-pyruvate is ionized form of this
2. Understand how the process ofalcoholic fermentation used
byyeast and some bacteria converts glucose to alcohol, carbon
dioxide, and ATP.
1. Be able to name factors that affect enzyme activity.2.
Understand how increasing number of respiring cells
increases the rate of respiration.3. Understand the concept of a
control in this experiment.3. Understand how to set up the yeast
fermentation experiment, and
how the data was graphed and interpreted.
4. Understand how the process ofaerobic respiration used by
most
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Cellular Respiration
organisms produce most of the ATP.
5. Understand how glycolysis is linked to the Krebs cycle.
1. Where does glycolysis occur? in the cytosol2. Where does
Krebs cycle occur? inner membrane of
mitochondria
6. Understand your aerobic respiration experiment. Understand
the
significance of the enzyme-catalyzed conversion of succinate
to
fumarate in the Krebs cycle.
7. Understand the following terms as they relate to your
aerobic
respiration experiment.
DCPIP % transmittance
Succinate
Fumarate
8. Understand the role substrate concentration plays with
respect
to the rate of an enzyme catalyzed reaction.
1. Experiment saturated with substrate
2. Experiment not saturated with substrate.
9. Be able to list and understand role of the components of
the
reaction tubes in the aerobic respiration experiment.Buffer
DCPIP
Mitochondrial suspensionSuccinate
11. Construct a graph of your data, and understand:1. Dependent
variable2. Independent variable3. Reason we plotted %
transmittance, and not absorbance for your
aerobic respiration data
12. During you lab preparation and study, work through the
above
objectives, student prelab preparation, lab summary questions
and
critical thinking questions at the end of the exercise.
