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  • Bioprocessos para el aprovechamiento de

    los desechos de cultivos multifunzionale

    Territorial and Production Systems Sustainability Department (SSPT)

    Biotechnology and Agroindustry Division

    Bioproducts and Bioprocesses Laboratory (PROBIO)

    Dr Loretta Bacchetta

  • Presentation outline

    Overview of the aims and activities at ENEA Biotechnologies and

    Agroindustry Division BIOAG– Bioprocess and Bioproducts Laboratory

    BIOAG inputs for cultural heritage conservation

    Examples of on-going research projects:

    Genetic resources characterization: hazelnuts as model of study

    Bioraffinery: Cynara spp. for food and non food applications

    Bio-fungicide: enhancement of buckwheat by-products

    The Mild technologies at PROBIO laboratory

    Efficient use of resources: biobased products from cladodi of Nopal for

    Cultural Heritage applications

    Final considerations

    2

    Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017

  • Biotechnology and Agroindustry Division-BIOAG

    3 Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017

     Focuses on innovation in the agro-industrial system for the development and competitiveness of food production in terms of quality, safety and sustainability, encouraging chain approaches, through agro-industrial and/or territory clusters.

     Promotes the development of products (food and non-food) with high-added value and high technological value derived from biological resources.

     Develops methods and technologies for innovation and traceability of the production, supply, processing and marketing chains of agricultural and food products in a perspective of enhancing consumer confidence in the foodchain.

    BIOTECHNOLOGY

    BIOPRODUCTS AND BIOPROCESSES

    SUSTAINABILITY, QUALITY AND SAFETY OF

    AGRIFOOD PRODUCTIONS

    All activities are performed through innovative infrastructures, equipment, facilities, and technology/service

    platforms, located in three different laboratories:

  • Biotechnology and Agroindustry Division activities

    Innovative biotechnology products and processes and systems for Bioeconomy

    • “Omic” sciences and new breeding techniques in Mediterranean crops

    • Conservation, characterization and evaluation of existing biological collections including microrganismsf or industrial purposes (food and biotechnology)

    • Enhancement of waste and agro-industrial by- products, into high added-value products (innovative food, feed, materials and bio-based products) and focusing on the resources efficient use, use of Key Enabling Technologies (KETs), industrial biotechnology (White Biotechnologies) and Process Technologies (with a special focus on Mild Technologies), for agroindustry and bio- industry

    Systems for sustinable quality safety of Agroindustrial productions

    • Conservative and precision agriculture for

    sustainability and phytosanitary measures for crop

    protection. Agriculture-based models, to improve

    the resilience to climate change and to promote

    reduction of inputs and greenhouse gas emissions

    • Precision indoor farming and agrospace

    • Use of renewable sources and improvement of

    the food system throughout a cascade-type

    approach, planning for maximal biomass

    exploitation and agri-food waste and for reducing

    food losses.

    • Validation and application of diagnostic methods

    for food safety, qualification of the productions

    ,processing, preservation and packaging. Quality

    markers for authenticity and origin of foods

    4 Bioprocesses– Meeting Ambasciata Italiana in Mexico Bilateral Project, November, 2017

  • "Bioproducts and Bioprocesses" Laboratory (PROBIO)

    5 Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017

    Aims to develop innovative products and processes in food and no-food areas

    through the use of Key Enabling Technologies (KETs). Special attention is devoted to

    developing industrial biotechnologies (White Biotechnologies) and Mild Technologies,

    in agroindustry and bio-industry areas.

    The activities are: to detection, at different levels of scale, of

    innovative and sustainable productive solutions, able to

    obtain a wide range of products /processes bio-based, with

    high added value of interest for new markets. The

    approaches allows the valorization of biological resources

    use, vegetables matrices, by-products and waste.

    The Laboratory combines expertise on valorization of vegetables, animal and microbial resources (agronomy,

    microbiology, entomology, chemistry, veterinary, environmental sciences), industrial biotechnology, chemical

    engineering, Nuclear Magnetic Resonance (NMR), biochemistry.

    sspt.enea.it

    roberto.balducchi@enea.it

  • BIOAG inputs for cultural heritage conservation

    6 Bio-processes– Meeting Ambasciata Italiana in Mexico Bilateral Project, November, 2017

    Biomortars

    Biocide additives

    Methodologies of chemical extraction –

    MILD TECNOLOGY

    Recovery biomolecules from waste

    Agro- biodiversity

  • Genetic resources characterization hazelnuts (Corylus avellana) as model of study

    7 Bioprocessi – Meeting Ambasciata Italiana in Mexico November2017

  • Corylus avellana variability

    SAFENUT: Agri Gen Res 068 Variability in polyphenolics

    8 Bioprocesses– Meeting Ambasciata Italiana in Messico, Bilateral Project, Novembre 2017

    215 cultivars e 58 accessions are amintaned in 13

    European collections

    190 are characterized by SSR.

    42 out of 140, ecotypes recovered ‘on farm’ were

    confirmed new genotypes

    18 phenolics identified in 53 cultivars: 10 flavonoidi (quercetin

    pentoside, quercetin 3-O-rhamnoside, catechin, epicatechin,

    myricetin-3-rhamnoside, procyanidin dimer-1, procyanidin dimer-2,

    procyanidin trimer-1, -2 and -3) 7 phenolics acids (gallic,

    protocatechuic, chlorogenic, p-coumaric, ferulic, ellagic and caffeic

    acid); 1 dihidrochalcone (phloridzin).

    Rovira M., et al, Bacchetta L.,2013. Annals and Nutrition metabolism 62 (2), 82 Boccacci P., et al., Bacchetta L., 2013. Tree Genetics & Genomes 9:1465-1480

  • Study of Corylus avellana variability

    Variabiliuty in fatty acids Variability in proteins and minerals

    9

    Bioprocessi – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembre 2017

    Analizzate 75 cultivars

    Bacchetta et al., 2013. Euphytica 191: 57-93 Drogoudi, P.,et al., Bacchetta, L, 2013. J. Food Sci. Nutr.,64, 202–209.

  • Fuctional food: clinical trials

    10 Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November,2017

    Asım Orem, et al. (2013). Hazelnut-enriched diet improves cardiovascular risk biomarkers beyond a lipid-lowering effect in

    hypercholesterolemic subjects. Journal of Clinical Lipidology, 7, 123-131.

    Tey, S.L., et al. (2011). Effects of different forms of hazelnuts on blood lipids and α-tocopherol concentrations in mildly

    hypercholesterolemic individuals. European Journal of Clinical Nutrition 65, 117–124.

    Mercanligil, S.M., et al. (2007). Effects of hazelnut-enriched diet on plasma cholesterol and lipoprotein profiles in

    hypercholesterolemic adult men. European Journal of Clinical Nutrition, 61, 212-220.

    Fraser, G.E., et al. (1992). A possible protective effect of hazelnut consumption on risk of coronary heart disease. The Adventist

    Health Study. Arch Intern Med, 152, 1416–1424

    BIOSERVICE Project

    Lr 13/2008 PROT. FILAS-

    RU-2014-1168 - 2018

    Activities

    CUORESANO

    Supported by

    COOPERNOCCIOLA srl

    (Viterbo)

  • Fuctional food: results of clinical trial

    11 Bioprocesses– Meeting Ambasciata Italiana in Mexico,

    Bilateral Project, November 2017

    + 251,2 Kcal/d

    No variation

    in weight

    reversible

    benefits

  • Improvements on chemical/molecular characterization

    Metabolomics ESOSOMES

    12 Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembre 2017

    Developped of protocol for isolation of esosomes

    ENEA e CNR –Biologia Cellulare e Neurobiologia

  • Genetic resources characterization: research activities in progress

    13 Bioprocesses – Meeting Ambasciata Italiana in Mexico,

    Bilateral Project, Novembre 2017

    RUTINA

    Gluten free

    Fagopyrum spp.

    esculentum tataricum

    Ocimum

    basilicum

    Cynara cardunculus var. scolymus

    Pinus pinea

    Brassica rapa local ecotypes

    sulforaphano (µ g/g ps)

    Bacchetta et al., 2016.

    A manifesto for the

    valorization of wild

    edible plants.

    J.Ethnopharmacology

    191:180-187

    Wild edible plants

    http://www.sciencedirect.com/science/journal/03788741 http: