Screening SetsInitial Screening Reagent I-VISystematically Controlled Crystallization Screen Set 101Sparse Matrix Screen Set I & II

器材AxyGem Crystallography PlateAxyPearl LidCrystalPlate-24, -48UltraClear Sealing Film

結晶化試薬バッファー

沈殿剤

塩

添加剤

界面活性剤

Crystallography Screening Sets＆ Plateタンパク質結晶化スクリーニングセット ＆プレート

スクリーニングセット選択ガイド

タンパク結晶化試薬条件最適化/フォローアップ

カスタムメード試薬

UniquePEG

Salt

Alcohol PresipitantsScreens(CP-CUSTOM-Ⅱ)

High ProbabilityPresipitants Screens

(CP-CUSTOM-Ⅰ)

Balanced PEG Screens

(CP-CUSTOM-Ⅲ)

SystemticallyControlled

Crystallization Screen Set

(CP-CUSTOM-101)

Highest Probability Salt

Screens(CP-CUSTOM-Ⅵ)

Balanced Salt Screens

(CP-CUSTOM-Ⅴ)

Highest Probability PEG

Screens(CP-CUSTOM-Ⅳ)

結晶化するタンパク質の性質が不明な場合は、まず、 High Probability Presipitants Screens (CP-

CUSTOM-Ⅰ)を、続いてAlcohl Presipitants Screens (CP-CUSTOM-Ⅱ)をお使い頂くことをお薦め致します。

これら2ｷｯﾄは相補的な組成となっております。結晶が認められない場合、 Balanced PEG Screens (CP-CUSTOM-Ⅲ)、 Highest Probability PEG Screens (CP-CUSTOM-Ⅳ)により検討頂くことをご提案致します。

更なる検討のために、スクリーニングセット（1ml x 96 Deep Well）のカスタムメードサービスも承っておりますので、お問い合わせ下さい。

1

Screening Sets

■ Initial Screening Sets （メーカーコードCP-CUSTOM-Ⅰ～Ⅵ）

ローレンスリバモア国立研究所により、開発された高効率タンパク質結晶化条件スクリーン用アルゴリズムCrysTool*)により規定されたコンポーネントで構成されています。CrysToolにより、Ｓａｌｔ濃度やpH値のタンパク質溶解性への影響などを最適化しデザインされています。本スクリーニングセットは一般的に見られるSparseマトリックス

スクリーニングと異なり、重複がなく、広範囲な条件で無駄なくスクリーニング頂くことができます。

*) AxyGeｎ Bioscience Inc.はCrysTool の世界的独占使用契約を結んで、使用しております。

CrysToolとは過去に多くの成功例を作り出してきたscreening by random combination (inspired by Charles Carter’s incomplete factorial and Jankarick and Kim’s sparse matrix)、footprint screening (inspired by Erico Stura’s footprint screen)やgrid screening (inspired by Alex McPherson’s grid screening)等の中でどれが

もっとも優れているかを評価する過程で開発された理論と手法です。

■ Systematically Contr olled Crystallization Screen Set 101 (メーカーコードCP-CUSTOM-101）

•High Probability Precipitants Screens (CP-CUSTOM-Ⅰ)•Alcohol Precipitating Screens (CP-CUSTOM-Ⅱ)•Balanced PEG Screens （CP-CUSTOM-Ⅲ)•Highest Probability PEG Screens （CP-CUSTOM-Ⅳ)•Balanced Salt Screens （CP-CUSTOM-Ⅴ）

•Highest Probability Salt Screens （CP-CUSTOM-Ⅵ)

Tranらによって検討1) された、Aramondo VillasenorとSeth Harris（Roshe)によってデザインされたスクリーンセットです。最も効率的な３種類の沈殿剤（硫酸アンモニウム、マロン酸塩、PEG)2)に重点をおいて、構成されています。それらの沈殿剤単独及び、第2選択的な沈殿剤（MPD, NaCl, PEG400, PEG8K, 2-プロパノール)との共存、

両方の組成から構成されています。

【特長】

統計的評価に有効な体系的な構成

A01-12ウェル：コントロールとして単一沈殿剤から構成

独自にデザインされたユニークな組成

Tranらによる24組成を含んでいます。

【参考文献】1) Tran et al: Acta Cryst., D60,1562 (2004).2) Peat et al: Acta Cryst., D61,1662 (2005).

パッケージサイズ ：2種類 （1ml x 96 Deep Well, 10ml ｘ 96 Tube）

パッケージサイズ ：2種類（ 1ml x 96 Deep Well、10ml ｘ 96 Tube）

■ Sparse Matr ix Screen Sets Ⅰ & Ⅱ （メーカーコード CP-CUSTOM-102 &-103)

スパースマトリックス法 1) 2) に基づいてデザインされたスクリーニングキットです。

1) Jancarik, J. and Kim, S.H., J. Appl. Cryst., 24, 409-411(1991). 2) Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y., and McPherson, A., Acta Cryst. D50, 414-423 (1994).

2

【 Screening Sets 価格表】

Initial Screening Sets

Systematically Controlled Crystallization Screen Set 101

Sparse Matrix Screen Sets Ⅰ & Ⅱ

★ ウェルの各組成の個別販売は行っておりません。一部の組成のみをご希望の際は、カスタムメードサービスをご利用下さい。

１Plate（96 well deep plate）中に、ご希望の組成をご希望のウェルにデザイン頂くことができます。

コードNo. メーカーコード 品名 容量希望納入価格

（円)

- - Custom Made Service, 1ml x 96 wells (deepwell plate) 1 Plate 50,000

メーカー指定の組成指定シート（エクセルファイル）にご記入頂きます。詳しくは、弊社販売店までお問い合わせ下さい。

コードNo. メーカーコード 品名 容量希望納入価格

（円)

516-53381 CP-CUSTOM-I-1ml High Probability Precipitating Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-I-10ml High Probability Precipitating Screens, 10ml x 96 tubes 1 Kit 105,000

513-53391 CP-CUSTOM-II-1ml Alcohol Precipitating Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-II-10ml Alcohol Precipitating Screens, 10ml x 96 tubes 1 Kit 105,000

516-53401 CP-CUSTOM-III-1ml Balanced PEG Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-III-10ml Balanced PEG Screens, 10ml x 96 tubes 1 Kit 105,000

513-53411 CP-CUSTOM-IV-1ml High Probability PEG Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-IV-10ml High Probability PEG Screens, 10ml x 96 tubes 1 Kit 105,000

510-53421 CP-CUSTOM-V-1ml Balanced Salt Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-V-10ml Balanced Salt Screens, 10ml x 96 tubes 1 Kit 105,000

517-53431 CP-CUSTOM-VI-1ml Highest Probability Salt Screens, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-VI-10ml Highest Probability Salt Screens, 10ml x 96 tubes 1 Kit 105,000

コードNo. メーカーコード 品名 容量希望納入価格

（円)

515-53351 CP-CUSTOM-101-1ml Crystallization Screen Set 101, 1ml x 96 wells (deepwell plate) 1 Plate 30,000

- CP-CUSTOM-101-10ml Crystallization Screen Set 101, 10ml x 96 tubes 1 Kit 105,000

コードNo. メーカーコード 品名 容量希望納入価格

（円)

512-53361 CP-CUSTOM-102 Sparse Matrix Screen Set I 10ml x50tubes 44,500

519-53371 CP-CUSTOM-103 Sparse Matrix Screen Set Ⅱ 10ml x48tubes 44,500

3

Screening Sets 組成表

High Probability Precipitating Screens （ CP-CUSTOM-I -1ml, -10ml)Well Precipitant pH Buffer pH Salt/Additive Additive 2 Detergent

A1 PEG 6k, 19.59% TRIS-Maleate, 0.10M 5.5 BOG, 0.35%A2 PEG-MME 5k, 25.85%A3 PEG 2k, 6.47%A4 PEG 2k, 14.26% BisTriPro, 0.10M 7.5A5 Na-Acetate, 0.63M Mg-Acetate, 0.13MA6 Mg-Formate, 0.38M Ethanol, 4.54%, MG8, 0.45%A7 Na-Malonate, 1.55M 6 TRIS-Maleate, 0.10M 5.5 Mg-Sulfate, 0.17M MG7, 0.48%A8 Na-Formate, 1.03M Na-Acetate, 0.10M 5.5A9 PEG-MME 2k, 6.65%A10 Na-K-Tartrate, 0.25M Imidazole-Maleate, 0.10M 6.5 Ethanol, 7.92%A11 Am-Sulfate, 1.20M Na-Succinate, 0.10M 7.5 LDAO, 0.96%A12 PEG-MME 5k, 27.49% Imidazole-Maleate, 0.10M 7.5B1 PEG-MME 2k, 8.51% TRIS-Maleate, 0.10M 6.5B2 PEG 4k, 16.63% Na-K-Phosphate, 0.10M 5.5 Hexanediol, 4.64%B3 PEG-MME 5k, 11.34% Glycerol, 4.33%B4 PEG 2k, 12.82%B5 Na-Chloride, 2.80M Na-Succinate, 0.10M 6.5 K-Chloride, 0.13MB6 Na-Malonate 1.06M 6 TRIS-HCl, 0.10M 7.5 Na-K-Phosphate, 0.13MB7 PEG-MME 5k, 5.20%, Na-Citrate, 0.10M 6.5 Am-Phosphate, 0.19M MG8, 0.79%B8 PEG 6k, 8.85% Isopropanol, 1.60%B9 Na-Malonate, 0.46M 7 HEPES, 0.10M 6.5 Na-K-Phosphate, 0.09M Glycerol, 3.93%B10 Am-Sulfate, 1.77M BisTriPro, 0.10M 5.5 MG8, 0.67%B11 Na-Chloride, 1.08M Mg-Acetate, 0.14MB12 PEG-MME 2k, 19.43%, EDTA, 0.01M BOG, 0.68%C1 PEG-MME 2k, 9.71% Am-Phosphate, 0.20MC2 PEG 4k, 20.52% HEPES, 0.10M 6.5C3 Na-K-Tartrate, 0.74M K-Chloride, 0.15M BOG, 0.96%C4 Na-Acetate, 2.03M BisTriPro, 0.10M 5.5C5 Na-Acetate, 1.66M HEPES, 0.10M 8.5 Glycerol, 7.74%C6 PEG 2k, 14.48% Glycerol, 6.45%C7 PEG 4k, 8.28% TRIS-Maleate, 0.10M, 6.5 MG8, 0.56%C8 Na-Malonate , 1.53M 6 Na-K-Phosphate, 0.10M 7.5 MG8, 0.23%C9 PEG-MME 2k, 25.81% Na-Succinate, 0.10M 6.5 Ethanol, 8.12%C10 PEG 2k, 7.78%C11 Na-Malonate, 1.47M 7 HEPES, 0.10M, 7.5 7.5 Glycerol, 1.18%C12 Na-K-Tartrate, 0.21M Na-K-Phosphate, 0.10M 8.5 LDAO, 0.84%D1 PEG 6k, 26.08%, HEPES, 0.10M 6.5 Am-Formate, 0.25M Glycerol, 5.34%D2 Na-Malonate, 0.51M 6 Glycerol, 6.04%D3 Mg-Formate, 0.56M Methanol, 3.12% LDAO, 0.64%D4 Na-Formate, 1.85MD5 PEG 6k, 15.02% Na-Citrate, 0.10M 6.5 MG8, 0.65%D6 Na-Formate, 1.27MD7 Na-Chloride, 2.98M Glycerol, 3.23%D8 Am-Sulfate, 0.79M Na-Acetate, 0.10M 5.5 Mg-Chloride, 0.16M MG8, 0.28%D9 PEG 4k, 27.61% TRIS-Maleate, 0.10M 4.5D10 Na-Malonate, 1.78M 7 TRIS-Maleate, 0.10M 5.5 Na-Citrate, 0.07M LDAO, 0.27%D11 Na-K-Tartrate, 1.11M LDAO, 0.41%D12 PEG 6k, 10.60% Na-Acetate, 0.10M 5.5 Hexanediol, 3.42% Glycerol, 2.96%E1 PEG-MME 5k, 28.41%E2 Na-K-Tartrate, 0.72M Imidazole-Maleate, 0.10M 6.5 Glycerol, 8.96% E3 Na-Malonate, 1.34M 7 Imidazole-Maleate, 0.10M 6.5E4 Na-Acetate, 0.96M Am-Phosphate, 0.17M MG8, 0.59%E5 PEG-MME 2k, 22.07% Glycerol, 5.50%E6 PEG-MME 2k, 11.05% HEPES, 0.10M 8.5 Ethanol, 1.51% Glycerol, 5.43%E7 PEG 2k, 19.03% Na-Acetate, 0.10M 5.5 Zn-Acetate, 0.01M Glycerol, 2.76%E8 Na-Acetate, 1.74M Ethanol, 4.85% MG7, 0.71%E9 Mg-Formate, 0.37M TRIS-Maleate, 0.10M 4.5 MG8, 0.27%E10 PEG 2k, 35.09% Ethanol, 1.21%E11 PEG 4k, 23.68% MG8, 0.23%E12 Na-Acetate, 1.34M Na-K-Phosphate, 0.10M 5.5 EDTA, 0.02M LDAO, 0.26%F1 PEG-MME 5k, 26.46% BisTriPro, 0.10M 7.5 Li-Sulfate, 0.03MF2 PEG 6k, 28.23% Na-Citrate, 0.03M Glycerol, 5.29%F3 Na-Malonate, 0.46M 7 Na-Citrate, 0.10M 5.5F4 Am-Sulfate, 0.70MF5 PEG 6k, 9.28% MG8, 0.55%F6 Na-Malonate, 1.93M 6 Na-Acetate, 0.10M 6.5 Glycerol, 7.84%F7 PEG 4k, 24.58% Na-Succinate, 0.10M 6.5 Isopropanol, 0.73%F8 Na-K-Tartrate, 0.62M TRIS-HCl, 0.10M 8.5F9 PEG-MME 5k, 22.02% TRIS-Maleate, 0.10M 5.5 2-Butanol, 4.59%F10 Na-Formate, 1.59M TRIS-HCl, 0.10M 8.5 Mg-Chloride, 0.18MF11 PEG-MME 2k, 24.44% MG8, 0.16%F12 PEG-MME 5k, 8.80% Hexanediol, 0.90% LDAO, 0.79%G1 Na-K-Tartrate, 0.81M Na-K-Phosphate, 0.10M, 6.5 Glycerol, 2.80%G2 PEG-MME 2k, 7.66% Glycerol, 9.75%G3 Na-Acetate, 1.90M TRIS-HCl, 0.10M 6.5 Methanol, 0.93%G4 PEG-MME 2k, 14.91% Na-K-Phosphate, 0.10M 8.5 Ethanol, 1.03%G5 Am-Sulfate, 0.86M LDAO, 0.92%G6 PEG 2k, 12.98% BisTriPro, 0.10M 7.5 Isopropanol, 9.11%G7 PEG-MME 5k, 28.74% BisTriPro, 0.10M 6.5G8 Na-Malonate, 0.46M 7 Na-Citrate, 0.10M 4.5 DMSO, 9.95% BOG, 0.23%G9 Na-K-Tartrate, 0.88M TRIS-Maleate, 0.10M 6.5 Glycerol, 4.24%G10 Na-Malonate, 0.40M 6 Na-Citrate, 0.10M 5.5 Hexanediol, 0.73%G11 PEG 2k, 27.21% TRIS-Maleate, 0.10M 6.5 Mg-Formate, 0.05MG12 PEG-MME 5k, 24.19% Imidazole-Maleate, 0.10M 8.5H1 PEG-MME 2k, 17.06% HEPES, 0.10M 7.5 Glycerol, 1.10%H2 PEG 2k, 27.31% MG7, 0.33%H3 PEG 4k, 16.66% Na-Acetate, 0.10M 6.5 BOG, 0.74%H4 Na-Formate, 0.44M TRIS-HCl, 0.10M 6.5 Na-K-Phosphate, 0.14MH5 Am-Sulfate, 1.33M Na-K-Phosphate, 0.10M 7.5 Isopropanol, 0.59% LDAO, 0.10%H6 Na-K-Tartrate, 0.65M Na-Succinate, 0.10 6.5 MG8, 0.74%H7 PEG-MME 2k, 12.58% TRIS-HCl, 0.10M 8.5H8 PEG 4k, 28.59% Na-K-Phosphate, 0.10M 7.5H9 PEG-MME 5k, 10.11%H10 Na-Formate, 0.65M DMSO, 8.45%H11 PEG-MME 2k, 12.49% Na-K-Phosphate, 0.10M 8.5 Glycerol, 8.08%H12 Mg-Formate, 0.17M TRIS-Maleate, 0.10M 5.5 Na-Acetate, 0.32M MG7, 0.91%

4

Alcohol Precipitating Screens (CP-CUSTOM-Ⅱ -1ml, -10ml)Well Precipitant pH Buffer pH Salt/Additive Additive 2 Detergent

A1 Hexanediol, 29.60% (%w/v) TRIS-Maleate, 0.10M 6.5 LDAO, 0.39% (%v/v)A2 MPD, 25.11% (%v/v) TRIS-HCl, 0.10M 7.5 Hexanediol, 0.78% (%w/v)A3 MPD, 44.62% (%v/v) Na-Citrate, 0.10M 6.5A4 Isopropanol, 46.99% (%v/v) Na-Succinate, 0.10M 4.5A5 MPD, 35.61% (%v/v) Na-Acetate, 0.10M 5.5 Ethanol, 5.84% (%v/v)A6 Isopropanol, 30.79% (%v/v)A7 Hexanediol, 33.23% (%w/v)A8 Hexanediol, 36.03% (%w/v)A9 Isopropanol, 40.04% (%v/v) Mg-Chloride, 0.07MA10 Hexanediol, 19.13% (%w/v) Na-K-Phosphate, 0.10M 5.5 Methanol, 1.31% (%v/v) Glycerol, 7.51% (%v/v)A11 MPD, 52.49% (%v/v) HEPES, 0.10M 7.5 Ethanol, 2.96% (%v/v) Glycerol, 9.46% (%v/v)A12 Hexanediol, 34.04% (%w/v)B1 MPD, 20.24% (%v/v) Na-Succinate, 0.10M 7.5 2-Butanol, 7.64% (%v/v) Glycerol, 4.67% (%v/v)B2 Ethanol, 21.83% (%v/v) BisTriPro, 0.10M 6.5B3 Ethanol, 48.44% (%v/v) Na-Acetate, 0.10M 5.5 Isopropanol, 8.09% (%v/v)B4 Ethanol, 40.65% (%v/v) TRIS-Maleate, 0.10M 5.5 K-Chloride, 0.29MB5 Isopropanol, 18.96% (%v/v) TRIS-Maleate, 0.10M 5.5 Li-Sulfate, 0.09MB6 Isopropanol, 42.29% (%v/v) Na-Citrate, 0.10M 4.5 Ethanol, 4.21% (%v/v) MG8, 0.91% (%w/v)B7 Isopropanol, 39.12% (%v/v) Na-Citrate, 0.10M 6.5B8 Ethanol, 24.12% (%v/v) Mg-Formate, 0.06MB9 Hexanediol, 6.08% (%w/v) Mg-Formate, 0.01M MG8, 0.60% (%w/v)B10 Isopropanol, 42.14% (%v/v) Hexanediol, 5.12% (%w/v) Glycerol, 1.72% (%v/v)B11 Hexanediol, 32.37% (%w/v) Na-Citrate, 0.10M 5.5 MG8, 0.92% (%w/v)B12 Ethanol, 57.11% (%v/v)C1 Isopropanol, 45.92% (%v/v) Na-Succinate, 0.10M 4.5C2 Ethanol, 14.89% (%v/v) Na-Citrate, 0.10M 5.5 Glycerol, 3.94% (%v/v)C3 Isopropanol, 19.98% (%v/v) TRIS-Maleate, 0.10M 6.5 LDAO, 0.38% (%v/v)C4 Isopropanol, 58.88% (%v/v) TRIS-Maleate, 0.10M 5.5 BOG, 0.14% (%w/v)C5 Hexanediol, 27.48% (%w/v)C6 Hexanediol, 26.57% (%w/v) Isopropanol, 1.90% (%v/v) MG7, 0.58% (%w/v)C7 MPD, 15.31% (%v/v) Methanol, 4.56% (%v/v) LDAO, 0.72% (%v/v)C8 MPD, 35.61% (%v/v) HEPES, 0.10M 8.5 Ca-Acetate, 0.08M MG8, 0.98% (%w/v)C9 Hexanediol, 8.39% (%w/v)C10 MPD, 22.84% (%v/v) Mg-Chloride, 0.38M MG7, 0.50% (%w/v)C11 Hexanediol, 24.21% (%w/v) Na-Citrate, 0.10M 7.5 Na-K-Phosphate, 0.22MC12 Ethanol, 21.20% (%v/v) Na-Succinate, 0.10M 5.5 BOG, 0.99% (%w/v)D1 Hexanediol, 10.02% (%w/v)D2 Isopropanol, 50.97% (%v/v) Imidazole-Maleate, 0.10M 7.5D3 MPD, 23.75% (%v/v) BisTriPro, 0.10M 6.5D4 Hexanediol, 11.19% (%w/v) TRIS-Maleate, 0.10M 6.5 MG8, 0.36% (%w/v)D5 Hexanediol, 36.75% (%w/v) TRIS-HCl, 0.10M 8.5D6 MPD, 32.19% (%v/v) Na-Citrate, 0.10M 4.5 LDAO, 0.85% (%v/v)D7 Isopropanol, 52.35% (%v/v) HEPES, 0.10M 7.5D8 Isopropanol, 51.57% (%v/v) Na-Citrate, 0.10M 5.5 LDAO, 0.55% (%v/v)D9 MPD, 20.73% (%v/v) MG7, 0.52% (%w/v)D10 Isopropanol, 49.06% (%v/v) Glycerol, 9.25% (%v/v)D11 Isopropanol, 38.99% (%v/v) Glycerol, 8.69% (%v/v)D12 Hexanediol, 21.08% (%w/v) TRIS-HCl, 0.10M 8.5 Na-K-Phosphate, 0.16M BOG, 0.61% (%w/v)E1 Ethanol, 29.98% (%v/v) Na-Acetate, 0.10M 6.5 Glycerol, 8.17% (%v/v)E2 Isopropanol, 28.66% (%v/v) Na-K-Phosphate, 0.10M 5.5 Glycerol, 1.34% (%v/v)E3 Isopropanol, 49.20% (%v/v) Mg-Chloride, 0.03M Glycerol, 8.66% (%v/v)E4 2-Butanol, 16.56% (%v/v) Na-Succinate, 0.10M 5.5E5 MPD, 52.67% (%v/v) Ca-Acetate, 0.09M LDAO, 0.88% (%v/v)E6 2-Butanol, 11.05% (%v/v) Na-Succinate, 0.10M 5.5 DMSO, 9.69% (%v/v)E7 Isopropanol, 10.36% (%v/v) TRIS-Maleate, 0.10M 6.5 Ca-Chloride, 0.17ME8 Isopropanol, 51.47% (%v/v) Imidazole-Maleate, 0.10M 7.5 Am-Formate, 0.13M Glycerol, 2.60% (%v/v)E9 Isopropanol, 25.67% (%v/v) TRIS-HCl, 0.10M 7.5 Am-Formate, 0.30M LDAO, 0.77% (%v/v)E10 MPD, 23.31% (%v/v) 2-Butanol, 2.81% (%v/v) LDAO, 0.29% (%v/v)E11 Ethanol, 52.38% (%v/v) Methanol, 7.65% (%v/v) LDAO, 0.44% (%v/v)E12 Isopropanol, 59.55% (%v/v) TRIS-HCl, 0.10M 6.5F1 MPD, 50.20% (%v/v) Na-Citrate, 0.10M 6.5F2 Ethanol, 27.67% (%v/v) Glycerol, 3.06% (%v/v)F3 Ethanol, 38.87% (%v/v) TRIS-HCl, 0.10M 8.5F4 Isopropanol, 53.97% (%v/v) BisTriPro, 0.10M 5.5 Mg-Chloride, 0.06M LDAO, 0.19% (%v/v)F5 Isopropanol, 30.47% (%v/v) TRIS-Maleate, 0.10M 4.5F6 Ethanol, 55.22% (%v/v) Na-K-Phosphate, 0.04M Glycerol, 7.92% (%v/v)F7 Hexanediol, 29.72% (%w/v) midazole-Maleate, 0.10M 5.5F8 Hexanediol, 10.80% (%w/v) Isopropanol, 4.76% (%v/v) Glycerol, 4.73% (%v/v)F9 Isopropanol, 59.72% (%v/v) DMSO, 9.07% (%v/v)F10 Isopropanol, 57.64% (%v/v) TRIS-Maleate, 0.10M 5.5F11 MPD, 36.39% (%v/v) Am-Formate, 0.09M MG8, 0.74% (%w/v)F12 Ethanol, 26.88% (%v/v) BisTriPro, 0.10M 6.5 LDAO, 0.95% (%v/v)G1 Hexanediol, 29.33% (%w/v) Imidazole-Maleate, 0.10M 6.5G2 Isopropanol, 52.18% (%v/v) LDAO, 0.73% (%v/v)G3 Isopropanol, 57% (%v/v) TRIS-Maleate, 0.10M 5.5 Am-Acetate, 0.26M LDAO, 0.20% (%v/v)G4 MPD, 38.56% (%v/v) MG8, 0.33% (%w/v)G5 MPD, 57.91% (%v/v) LDAO, 0.71% (%v/v)G6 Isopropanol, 14.24% (%v/v) HEPES, 0.10M 6.5 2-Butanol, 1.99% (%v/v)G7 Hexanediol, 30.28% (%w/v) Ethanol, 8.30% (%v/v)G8 Hexanediol, 32.75% (%w/v)G9 MPD, 31.70% (%v/v) Na-Citrate, 0.10M 5.5 LDAO, 0.65% (%v/v)G10 Ethanol, 46.16% (%v/v) Na-Acetate, 0.10M 4.5 Isopropanol, 0.66% (%v/v)G11 Isopropanol, 30.32% (%v/v) Na-Citrate, 0.10M 4.5 MG8, 0.16% (%w/v)G12 Isopropanol, 10.45% (%v/v) BisTriPro, 0.10M 7.5 DMSO, 7.24% (%v/v) Glycerol, 4.22% (%v/v)H1 Isopropanol, 41.18% (%v/v) Glycerol, 9.96% (%v/v)H2 Ethanol, 20.59% (%v/v) Na-Citrate, 0.10M 5.5 Glycerol, 5.64% (%v/v)H3 Hexanediol, 23.38% (%w/v) TRIS-Maleate, 0.10M 5.5H4 Hexanediol, 39.22% (%w/v) TRIS-HCl, 0.10M 7.5 Na-Acetate, 0.08M BOG, 0.78% (%w/v)H5 MPD, 46.34% (%v/v)H6 Isopropanol, 24.27% (%v/v)H7 Isopropanol, 20.65% (%v/v) Na-Acetate, 0.10M 6.5 Am-Formate, 0.42M Glycerol, 2.56% (%v/v)H8 MPD, 26.46% (%v/v) Imidazole-Maleate, 0.10M 5.5 DMSO, 6.84% (%v/v)H9 MPD, 30.41% (%v/v) TRIS-HCl, 0.10M 8.5H10 Hexanediol, 32.32% (%w/v) Na-Acetate, 0.10M 5.5 Am-Formate, 0.06M BOG, 0.80% (%w/v)H11 Isopropanol, 50.01% (%v/v) HEPES, 0.10M 7.5 DMSO, 1.41% (%v/v) Glycerol, 9.63% (%v/v)H12 Hexanediol, 31.68% (%w/v) Na-K-Phosphate, 0.10M 5.5

5

Balanced PEG Screens （CP-CUSTOM-Ⅲ-1ml, -10ml)Well Precipitant pH Buffer pH Salt/Additive Additive 2 Detergent

A1 PEG 10k, 17.14% LDAO, 0.95%A2 PEG 400, 38.12% Glycerol, 9.60%A3 PEG 400, 25.37% BisTriPro, 0.10M 8.5A4 PEG 10k, 10.35% Imidazole-Maleate, 0.10M 4.5A5 PEG-MME 750, 25.37% Na-Citrate, 0.10M 4.5A6 PEG 10k, 13.43% Am-Acetate, 0.35M SBOG, 0.29%A7 PEG-MME 2k, 19.01% EDTA, 0.01MA8 PEG 2k, 12.86% HEPES, 0.10M 7.5 Am-Acetate, 0.13M LDAO, 0.24%A9 PEG 400, 50.74% Na-K-Phosphate, 0.10M 8.5 MG8, 0.12%A10 PEG-MME 750, 21.92% TRIS-HCl, 0.10M 7.5 Am-Phosphate, 0.15MA11 PEG 2k, 12.81% LDAO, 0.51%A12 PEG-MME 750, 18.89% TRIS-Maleate, 0.10M 5.5 Na-Citrate, 0.10MB1 PEG 4k, 27.23% Imidazole-Maleate, 0.10M 7.5B2 PEG 400, 58.77% TRIS-HCl, 0.10M 7.5B3 PEG 400, 24.29% Na-Acetate, 0.10M 6.5B4 PEG 6k, 18.42% Glycerol, 2.92%B5 PEG 10k, 15.37% Na-Acetate, 0.10M 6.5B6 PEG-MME 2k, 18.51% LDAO, 0.34%B7 PEG-MME 750, 13.92%B8 PEG-MME 5k, 25.07%B9 PEG 10k, 13.93%B10 PEG 2k, 28.64% TRIS-HCl, 0.10M 8.5B11 PEG 4k, 12.68% Na-Acetate, 0.10M 4.5 Am-Acetate, 0.17MB12 PEG 6k, 14.26% TRIS-Maleate, 0.10M 5.5 Am-Phosphate, 0.16MC1 PEG 10k, 11.40% TRIS-Maleate, 0.10M 5.5 Glycerol, 1.22%C2 PEG 10k, 16.80% Na-Succinate, 0.10M 4.5C3 PEG 10k, 13.53% TRIS-Maleate, 0.10M 5.5C4 PEG 10k, 15.99% Na-K-Phosphate, 0.10M 8.5C5 PEG-MME 2k, 13.87%C6 PEG-MME 750, 16.79% BisTriPro, 0.10M 7.5C7 PEG 400, 34.25% TRIS-HCl, 0.10M 6.5 SBOG, 0.72%C8 PEG 10k, 10.22% Na-K-Phosphate, 0.10M 6.5C9 PEG-MME 750, 24.06% Glycerol, 8.22%C10 PEG 10k, 10.98% Na-Acetate, 0.10M 4.5C11 PEG 10k, 13.35% Na-Citrate, 0.10M 7.5 Glycerol, 8.08%C12 PEG 10k, 17.57% LDAO, 0.32%D1 PEG-MME 750, 15.73% Na-Acetate, 0.10M 4.5D2 PEG-MME 750, 21.17% BisTriPro, 0.10M 7.5 Glycerol, 6.36%D3 PEG 10k, 11.30% Na-Acetate, 0.10M 6.5D4 PEG 400, 20.78% TRIS-HCl, 0.10M 6.5 LDAO, 0.59%D5 PEG 400, 33.39% TRIS-Maleate, 0.10M 5.5 Glycerol, 1.02%D6 PEG 10k, 11.02% TRIS-HCl, 0.10M 8.5 MG7, 0.75%D7 PEG 400, 43.51% Na-K-Phosphate, 0.10M 7.5D8 PEG 10k, 10.45%D9 PEG 10k, 10.22% HEPES, 0.10M 6.5D10 PEG 10k, 13.81% Na-Acetate, 0.10M 4.5D11 PEG-MME 750, 25.99%D12 PEG 10k, 17.59% BisTriPro, 0.10M 8.5 Na-Acetate, 0.29ME1 PEG-MME 5k, 18.33% Na-K-Phosphate, 0.10M 6.5 Glycerol, 8.25%E2 PEG 400, 22.16% Na-K-Phosphate, 0.10M 6.5 Na-K-Tartrate, 0.17M LDAO, 0.17%E3 PEG 10k, 11.89% Imidazole-Maleate, 0.10M 8.5E4 PEG-MME 2k, 20.63%E5 PEG-MME 5k, 20.62% Imidazole-Maleate, 0.10M 4.5 Na-Acetate, 0.05ME6 PEG 10k, 11.81% TRIS-HCl, 0.10M 6.5 Am-Sulfate, 0.12ME7 PEG-MME 750, 24.12% Na-Citrate, 0.10M 5.5 Glycerol, 2.14%E8 PEG 400, 33.41% BisTriPro, 0.10M 5.5E9 PEG-MME 750, 14.45% Glycerol, 8.66%E10 PEG 400, 56.11% Na-K-Phosphate, 0.10M 6.5 EDTA, 0.02ME11 PEG 10k, 12.44% Na-Succinate, 0.10M 6.5 K-Chloride, 0.16ME12 PEG 10k, 13.17% Na-K-Tartrate, 0.15M MG8, 0.89%F1 PEG 10k, 17.26%F2 PEG 6k, 14.45% Na-Acetate, 0.26M LDAO, 0.60%F3 PEG-MME 5k, 12.17% Na-Citrate, 0.10M 6.5F4 PEG-MME 2k, 25.17% HEPES, 0.10M 7.5F5 PEG-MME 750, 18.10%F6 PEG-MME 750, 28.88% Na-Citrate, 0.10M 4.5F7 PEG 4k, 10.34%F8 PEG 400, 59.20% Imidazole-Maleate, 0.10M 6.5 EDTA, 0.01MF9 PEG 10k, 10.30% Na-Acetate, 0.10M 5.5F10 PEG 400, 37.36% Na-K-Phosphate, 0.10M 6.5F11 PEG 10k, 15.45% TRIS-Maleate, 0.10M 6.5 Am-Sulfate, 0.06MF12 PEG 10k, 13.20% Li-Sulfate, 0.18MG1 PEG 400, 57.15% Li-Sulfate, 0.06M Glycerol, 7.22%G2 PEG 6k, 27.84% Na-Acetate, 0.10M 6.5 MG7, 0.94%G3 PEG 2k, 21.93% Imidazole-Maleate, 0.10M 8.5G4 PEG 400, 31.87% BisTriPro, 0.10M 8.5G5 PEG 10k, 13.55% Na-Citrate, 0.10M 6.5 Glycerol, 4.36%G6 PEG 4k, 29.46% Na-Citrate, 0.10M 4.5G7 PEG 6k, 27.29% TRIS-HCl, 0.10M 7.5G8 PEG-MME 750, 33.30% Imidazole-Maleate, 0.10M 7.5 Mg-Formate, 0.06M BOG, 0.95%G9 PEG 400, 32.79%G10 PEG 400, 34.53% Mg-Formate, 0.03MG11 PEG-MME 750, 25.10% HEPES, 0.10M 6.5 MG7, 0.95%G12 PEG 400, 25.61% Na-Citrate, 0.10M 4.5H1 PEG-MME 5k, 26.46% BisTriPro, 0.10M 5.5H2 PEG-MME 750, 26.37% TRIS-Maleate, 0.10M 6.5 Glycerol, 6.37%H3 PEG-MME 2k, 30.46% Na-Citrate, 0.10M 5.5 K-Chloride, 0.32M MG8, 0.16%H4 PEG-MME 750, 19.64% Imidazole-Maleate, 0.10M 4.5 Zn-Acetate, 0.06MH5 PEG 6k, 15.86% Na-Citrate, 0.10M 4.5H6 PEG-MME 2k, 27.23% Na-Acetate, 0.10M 6.5 MG7, 0.50%H7 PEG 10k, 10.84% TRIS-Maleate, 0.10M 6.5 Mg-Chloride, 0.08MH8 PEG 10k, 13.44% TRIS-Maleate, 0.10M 6.5H9 PEG 4k, 14.48% Na-Acetate, 0.10M 5.5H10 PEG 10k, 16.67% Na-Citrate, 0.10M Glycerol, 3.69%H11 PEG-MME 750, 35.13% Na-K-Tartrate, 0.11MH12 PEG-MME 5k, 15.78% Na-K-Phosphate, 0.10M 8.5 EDTA, 0.01M

6

Highest Probability PEG Screens （CP-CUSTOM-Ⅳ-1ml, -10ml)

Well Precipitant pH Buffer pH Salt/Additive Additive 2 DetergentA1 PEG-MME 2k, 12.30%A2 PEG-MME 2k, 17.44% Na-Succinate, 0.1M 7.5A3 PEG 4k, 12.36% Na-Succinate, 0.10M 5.5A4 PEG 2k, 20.91% BOG, 0.80%A5 PEG-MME 2k, 15.58% Imidazole-Maleate, 0.10M 8.5 Glycerol, 9.98%A6 PEG 2k, 16.27%A7 PEG-MME 5k, 15.53%A8 PEG 4k, 10.57% Am-Formate, 0.43MA9 PEG-MME 2k, 32.08%A10 PEG 6k, 28.43% Na-Citrate, 0.10M 7.5A11 PEG-MME 2k, 23.97% TRIS-HCl, 0.10M 7.5A12 PEG-MME 2k, 27.85% Na-Acetate, 0.10M 4.5 Mg-Chloride, 0.03MB1 PEG-MME 5k, 26.78%B2 PEG 4k, 17.72% TRIS-Maleate, 0.10M 5.5 Mg-Sulfate, 0.08MB3 PEG-MME 2k, 14.61% Na-Acetate, 0.10M 6.5B4 PEG-MME 2k, 33.68% Na-K-Phosphate, 0.10M 5.5 Am-Phosphate, 0.16M Glycerol, 5.52%B5 PEG-MME 2k, 20.87% Na-K-Phosphate, 0.10M 5.5B6 PEG 6k, 19.72% TRIS-HCl, 0.10M 7.5B7 PEG-MME 5k, 17.49% Imidazole-Maleate, 0.10M 4.5B8 PEG-MME 2k, 21.51% Na-K-Phosphate, 0.10M 8.5 Na-Citrate, 0.04MB9 PEG-MME 2k, 24.93% Na-Succinate, 0.10M 6.5 Na-Citrate, 0.05MB10 PEG-MME 2k, 16.18% Imidazole-Maleate, 0.10M 7.5 Glycerol, 8.90%B11 PEG 6k, 24.34% Zn-Acetate, 0.08MB12 PEG-MME 5k, 13.00% Na-Acetate, 0.10M 5.5 Na-K-Phosphate, 0.06MC1 PEG 6k, 18.22% BisTriPro, 0.10M 7.5 Mg-Acetate, 0.03MC2 PEG-MME 2k, 27.50% MG7, 0.69%C3 PEG 2k, 29.81% Imidazole-Maleate, 0.10M 6.5 Am-Sulfate, 0.18MC4 PEG 4k, 17.01% TRIS-HCl, 0.10M 7.5C5 PEG 4k, 12.31% HEPES, 0.10M 7.5 Na-Acetate, 0.13MC6 PEG 6k, 27.80% TRIS-HCl, 0.10M 6.5C7 PEG-MME 2k, 18.41% TRIS-Maleate, 0.10M 6.5 Mg-Chloride, 0.09MC8 PEG-MME 2k, 22.35% TRIS-HCl, 0.10M 7.5C9 PEG-MME 2k, 15.66% Na-Citrate, 0.10M 4.5C10 PEG 6k, 29.04% Na-K-Phosphate, 0.10M 5.5 MG8, 0.15%C11 PEG 2k, 26.44% TRIS-HCl, 0.10M 6.5 MG8, 0.92%C12 PEG-MME 2k, 23.37% EDTA, 0.02MD1 PEG 2k, 14.91% Mg-Chloride, 0.09MD2 PEG-MME 5k, 22.67% TRIS-Maleate, 0.10M 6.5 Am-Phosphate, 0.19MD3 PEG 4k, 10.46% Na-Acetate, 0.10M 6.5 EDTA, 0.01MD4 PEG 4k, 17.24% BisTriPro, 0.10M 5.5 Ca-Acetate, 0.09MD5 PEG 2k, 35.66% Na-K-Phosphate, 0.08M Glycerol, 2.16%D6 PEG-MME 2k, 12.03% TRIS-HCl, 0.10M 6.5 Glycerol, 3.49%D7 PEG 2k, 21.51% Na-Succinate, 0.10M 7.5D8 PEG 2k, 33.00% TRIS-HCl, 0.10M 6.5D9 PEG 6k, 26.43% BisTriPro, 0.10M 5.5D10 PEG 2k, 19.24% HEPES, 0.10M 6.5 BOG, 0.88%D11 PEG 2k, 16.42%D12 PEG 6k, 26.85% TRIS-Maleate, 0.10M 6.5 Glycerol, 9.26%E1 PEG 6k, 12.09% Imidazole-Maleate, 0.10M 5.5E2 PEG-MME 2k, 16.30%E3 PEG-MME 2k, 35.50% TRIS-Maleate, 0.10M 4.5 Am-Formate, 0.29ME4 PEG-MME 2k, 20.79% TRIS-HCl, 0.10M 8.5E5 PEG 4k, 20.93% Imidazole-Maleate, 0.10M 8.5 Na-Citrate, 0.08M MG8, 0.99%E6 PEG-MME 5k, 27.23%E7 PEG-MME 2k, 14.98% Na-Succinate, 0.10M 6.5E8 PEG 2k, 15.64% Na-Succinate, 0.10M 5.5 Am-Sulfate, 0.32ME9 PEG 2k, 26.64% Glycerol, 5.98%E10 PEG 4k, 15.53% BisTriPro, 0.10M 5.5 Li-Sulfate, 0.10ME11 PEG-MME 2k, 21.27% Na-Acetate, 0.10M 4.5E12 PEG 6k, 23.28% Na-Citrate, 0.10M 6.5F1 PEG-MME 5k, 13.86% Na-Acetate, 0.10M 6.5F2 PEG 2k, 31.18% Na-Citrate, 0.08MF3 PEG 2k, 32.43% Na-Acetate, 0.10M 6.5 MG8, 0.24%F4 PEG 4k, 28.11% Mg-Chloride, 0.17MF5 PEG-MME 2k, 18.60% BisTriPro, 0.10M 6.5 Glycerol, 9.23%F6 PEG 6k, 23.35% TRIS-HCl, 0.10M 6.5F7 PEG 2k, 12.76% Li-Sulfate, 0.03MF8 PEG 6k, 24.18% MG8, 0.71%F9 PEG 6k, 12.61% Na-Acetate, 0.10M 5.5F10 PEG-MME 2k, 18.57% Na-Acetate, 0.10M 5.5F11 PEG 2k, 22.11% Imidazole-Maleate, 0.10M 6.5F12 PEG-MME 5k, 17.30% TRIS-Maleate, 0.10M 6.5 Am-Formate, 0.10MG1 PEG-MME 2k, 18.44% Na-Succinate, 0.10M 6.5 Glycerol, 6.32%G2 PEG-MME 5k, 12.58% Na-K-Phosphate, 0.10M 6.5 Glycerol, 9.25%G3 PEG 2k, 26.75% TRIS-HCl, 0.10M 8.5G4 PEG-MME 2k, 25.90% BisTriPro, 0.10M 8.5G5 PEG-MME 5k, 27.18% BisTriPro, 0.10M 6.5 Na-Citrate, 0.03MG6 PEG 2k, 21.70% Na-K-Phosphate, 0.10M 5.5G7 PEG-MME 5k, 11.79%G8 PEG-MME 2k, 24.27% TRIS-Maleate, 0.10M 5.5G9 PEG-MME 2k, 25.89% Na-Chloride, 0.30MG10 PEG 6k, 10.22% BisTriPro, 0.10M 5.5G11 PEG 2k, 24.56% Na-Succinate, 0.10M 6.5 MG7, 0.97%G12 PEG 2k, 26.97% TRIS-HCl, 0.10M 8.5 Glycerol, 4.46%H1 PEG 4k, 10.67% Na-K-Phosphate, 0.10M 7.5 Am-Formate, 0.49MH2 PEG-MME 5k, 11.09% BisTriPro, 0.10M 5.5H3 PEG-MME 5k, 14.37% Na-Citrate, 0.10M 5.5 Na-Chloride, 0.22MH4 PEG-MME 2k, 34.81% Glycerol, 3.44%H5 PEG-MME 2k, 28.57% Na-Succinate, 0.10M 4.5 Glycerol, 2.04%H6 PEG 6k, 19.17% Na-Citrate, 0.10M 6.5H7 PEG 6k, 14.79% Imidazole-Maleate, 0.10M 6.5H8 PEG-MME 2k, 20.01% Am-Sulfate, 0.13MH9 PEG-MME 2k, 28.61% Glycerol, 3.03%H10 PEG-MME 2k, 24.75% HEPES, 0.10M 7.5H11 PEG-MME 2k, 29.82% HEPES, 0.10M 6.5 Glycerol, 2.34%H12 PEG 4k, 28.84% HEPES, 0.10M 7.5

7

Balanced Salt Screens （CP-CUSTOM-Ⅴ-1ml, -10ml ）Well Precipitant pH Buffer pH Salt/Additive Additive 2 Detergent

A1 Mg-Acetate, 1.23M Na-Acetate, 0.10M 6.5A2 Mg-Sulfate, 0.56M HEPES, 0.10M 8.5A3 Na-Acetate, 1.04M Na-K-Phosphate, 0.10M 6.5 MG7, 0.12%A4 Na-K-Tartrate, 0.34M HEPES, 0.10M 7.5A5 Na-Citrate, 0.38M Imidazole-Maleate, 0.10M 5.5A6 Na-Malonate, 0.72M 6 Imidazole-Maleate, 0.10M 5.5 Am-Phosphate, 0.18M BOG, 0.14%A7 Na-K-Tartrate, 1.03M Glycerol, 1.25%A8 Mg-Acetate, 0.84M BisTriPro, 0.10M 8.5 Mg-Chloride, 0.02MA9 Na-Acetate, 2.08MA10 Mg-Chloride, 2.29M Na-Acetate, 0.10M 5.5 Glycerol, 9.92%A11 Na-Citrate, 1.16MA12 Mg-Acetate, 1.18M Mg-Sulfate, 0.17MB1 Mg-Formate, 0.41MB2 Na-Malonate 0.37M 6 Na-Acetate, 0.10M 6.5 Glycerol, 6.70%B3 Am-Phosphate, 1.29M Imidazole-Maleate, 0.10M 6.5 Na-K-Phosphate, 0.21M LDAO, 0.12%B4 Na-Acetate, 1.23MB5 Na-Formate, 2.30M Na-K-Phosphate, 0.10M 8.5 MG8, 0.34%B6 Na-K-Tartrate, 1.07MB7 Na-K-Phosphate, 2.35M TRIS-Maleate, 0.10M 6.5 Na-Citrate, 0.01MB8 Na-Acetate, 1.56M HEPES, 0.10M 8.5 LDAO, 0.79%B9 Na-Citrate, 0.82M TRIS-Maleate, 0.10M 5.5 Na-Acetate, 0.30MB10 Na-Citrate, 0.64M Na-K-Phosphate, 0.10M 7.5 Glycerol, 4.92%B11 Na-Formate, 1.89MB12 Mg-Sulfate, 1.25M HEPES, 0.10M 6.5C1 Na-K-Tartrate, 0.49M Imidazole-Maleate, 0.10M 6.5 Glycerol, 9.80%C2 Na-Acetate, 2.63M Na-Succinate, 0.10M 7.5 Mg-Sulfate, 0.02MC3 Na-Malonate, 1.04M 6 TRIS-HCl, 0.10M 8.5C4 K-Chloride, 1.71M Na-Citrate, 0.10M 7.5 MG8, 1.00%C5 Mg-Formate, 0.29M Ca-Chloride, 0.22M Glycerol, 2.25%C6 Li-Sulfate, 0.76M Imidazole-Maleate, 0.10M 5.5C7 Na-Malonate, 1.38M 7 HEPES, 0.10M 8.5 LDAO, 0.40%C8 Na-Formate, 2.43M Na-Acetate, 0.10M 4.5 LDAO, 0.16%C9 Na-Chloride, 3.49M Na-K-Phosphate, 0.10M 5.5C10 Na-Citrate, 0.98M TRIS-Maleate, 0.10M 6.5 Am-Phosphate, 0.15MC11 Na-Formate, 0.82M BisTriPro, 0.10M 6.5 K-Chloride, 0.10M LDAO, 0.21%C12 Mg-Acetate, 0.58M BisTriPro, 0.10M 6.5 Am-Sulfate, 0.07MD1 Mg-Acetate, 0.34M Na-Acetate, 0.10M 5.5 Mg-Sulfate, 0.06M MG8, 0.72%D2 Am-Sulfate, 1.34M Glycerol, 4.52%D3 Na-Malonate, 0.69M 6 Na-Citrate, 0.10M 5.5D4 Na-Acetate, 0.86M K-Chloride, 0.13M LDAO, 0.39%D5 Na-Citrate, 0.31M HEPES, 0.10M 8.5 Am-Formate, 0.09MD6 Na-Malonate, 0.51M 7 Na-Succinate, 0.10M 5.5D7 Na-Citrate, 1.19M BOG, 0.15%D8 Na-Formate, 0.98M Na-K-Phosphate, 0.22M MG7, 0.13%D9 Na-Citrate, 0.31M Na-K-Phosphate, 0.10M 5.5D10 Na-K-Tartrate, 0.49MD11 Na-Malonate, 0.83M 7 HEPES, 0.10M 8.5 Mg-Sulfate, 0.12MD12 Na-K-Tartrate, 0.93ME1 Na-K-Tartrate, 1.36M TRIS-Maleate, 0.10M 6.5E2 Mg-Acetate, 0.92M BisTriPro, 0.10M 5.5E3 Am-Phosphate, 0.51M, LDAO, 0.25%E4 Mg-Formate, 0.61M Glycerol, 4.47%E5 Am-Sulfate, 0.37M Na-Succinate, 0.10M 7.5 Glycerol, 1.83%E6 Na-Chloride, 3.07M Mg-Chloride, 0.28M Glycerol, 7.79%E7 Na-K-Tartrate, 0.95M Imidazole-Maleate, 0.10M 8.5E8 Na-K-Tartrate, 0.34M Na-Acetate, 0.10M 6.5 Am-Acetate, 0.28M Glycerol, 5.91%E9 Na-K-Tartrate, 1.31M MG7, 0.57%E10 Na-Citrate, 0.97M Imidazole-Maleate, 0.10M 4.5 EDTA, 0.01ME11 Na-Malonate, 0.74M 7 TRIS-HCl, 0.10M 7.5 MG8, 0.56%E12 Na-Malonate, 2.03M 6 Na-Citrate, 0.10M 4.5F1 Mg-Chloride, 0.42M Imidazole-Maleate, 0.10M 7.5 LDAO, 0.16%F2 Na-Formate, 2.25M Na-Citrate, 0.10M 4.5 LDAO, 0.17%F3 Mg-Chloride, 2.05M LDAO, 0.88%F4 Mg-Acetate, 1.19MF5 Am-Phosphate, 1.22MF6 Na-K-Phosphate, 1.89M HEPES, 0.10M 6.5F7 Na-K-Tartrate, 1.39MF8 Mg-Sulfate, 0.80M BisTriPro, 0.10M 8.5 Na-Citrate, 0.02M MG8, 0.72%F9 Na-Citrate, 0.42M Na-K-Phosphate, 0.10M 6.5F10 Am-Phosphate, 0.93M TRIS-Maleate, 0.10M 6.5 MG8, 0.14%F11 Na-Citrate, 1.08M Na-Succinate, 0.10M 6.5 Glycerol, 4.98%F12 Na-K-Tartrate, 0.86MG1 Na-Chloride, 1.17M Imidazole-Maleate, 0.10M 7.5 LDAO, 0.65%G2 Na-K-Phosphate, 2.93MG3 K-Chloride, 1.82MG4 K-Chloride, 0.73M Na-Citrate, 0.10M 6.5 LDAO, 1.00%G5 Na-K-Tartrate, 0.73M Imidazole-Maleate, 0.10M 6.5G6 Na-K-Tartrate, 0.36M Na-Citrate, 0.10M 6.5 LDAO, 0.99%G7 Na-K-Phosphate, 2.49M Na-Citrate, 0.10M 4.5 Glycerol, 3.24%G8 Mg-Formate, 0.49M BisTriPro, 0.10M 8.5 Glycerol, 3.60%G9 Na-K-Tartrate, 1.08M BisTriPro, 0.10M 8.5G10 Na-Malonate, 2.13M 7 LDAO, 0.43%G11 Na-K-Tartrate, 0.61M Na-K-Phosphate, 0.10M 7.5 Am-Phosphate, 0.04M LDAO, 0.12%G12 Li-Sulfate, 0.31MH1 Li-Sulfate, 0.70M LDAO, 0.76%H2 Na-Malonate, 1.45M 7 TRIS-Maleate, 0.10M 4.5H3 Mg-Formate, 0.49M Ca-Chloride, 0.03MH4 Am-Sulfate, 0.40M TRIS-HCl, 0.10M 8.5H5 Na-K-Tartrate, 1.22M Imidazole-Maleate, 0.10M 6.5H6 Na-K-Tartrate, 1.02M HEPES, 0.10M 6.5 LDAO, 0.23%H7 Mg-Chloride, 0.83M Glycerol, 3.45%H8 K-Chloride, 2.08M TRIS-HCl, 0.10M 8.5H9 Li-Sulfate, 0.27M BisTriPro, 0.10M 5.5H10 Na-Malonate, 0.85M 7 Imidazole-Maleate, 0.10M 7.5 Na-K-Tartrate, 0.08M MG8, 0.67%H11 Na-Formate, 1.04MH12 Mg-Chloride, 1.40M BOG, 0.81%

8

Highest Probability Salt Screens （CP-CUSTOM-Ⅵ-1ml, -10ml)Well Precipitant pH Buffer pH Salt/Additive Additive 2 Detergent

A1 Na-Malonate, 0.72M 7 Na-K-Phosphate, 0.10M 6.5 MG7, 0.84%A2 Na-K-Tartrate, 1.24M BisTriPro, 0.10M 6.5A3 Mg-Chloride, 1.93MA4 Am-Sulfate, 1.66M HEPES, 0.10M 8.5A5 Na-Malonate, 1.97M 6 Mg-Sulfate, 0.07M LDAO, 0.66%A6 Na-Formate, 0.67M Mg-Chloride, 0.08MA7 Na-K-Phosphate, 1.43M HEPES, 0.10M 8.5 Glycerol, 9.34%A8 Na-Citrate, 0.71M TRIS-Maleate, 0.10M 5.5 Glycerol, 1.45%A9 Mg-Formate, 0.28M TRIS-HCl, 0.10M 7.5A10 Na-K-Tartrate, 1.34M TRIS-HCl, 0.10M 6.5A11 Na-Malonate , 1.76M 7 MG7, 0.54%A12 Na-Formate, 0.57M HEPES, 0.10M 8.5 Glycerol, 3.29%B1 Na-K-Phosphate, 2.35M HEPES, 0.10M 6.5B2 Na-Malonate, 0.53M 6 TRIS-HCl, 0.10M 8.5B3 Am-Sulfate, 2.32M LDAO, 0.80%B4 Na-Malonate, 0.87M 6B5 Na-Formate, 2.43M HEPES, 0.10M 6.5 Mg-Chloride, 0.23M LDAO, 0.63%B6 Na-Citrate, 0.58M Na-Acetate, 0.10M 5.5 Am-Phosphate, 0.20M BOG, 0.13%B7 Am-Sulfate, 0.54M LDAO, 0.67%B8 Na-Chloride, 2.29M TRIS-Maleate, 0.10M 6.5B9 Na-Citrate, 1.17M Na-Succinate, 0.10M 7.5 MG8, 0.66%B10 Na-Malonate, 0.91M 7 Glycerol, 9.56%B11 Na-Malonate, 1.33M 7B12 Am-Sulfate, 1.21M Na-Citrate, 0.10M 7.5C1 Na-K-Tartrate, 0.65M Na-Succinate, 0.10M 6.5 MG8, 0.35%C2 Na-Acetate, 2.52M Mg-Formate, 0.04M BOG, 0.59%C3 Na-Acetate, 1.14M TRIS-Maleate, 0.10M 6.5 Na-Citrate, 0.08MC4 Mg-Formate, 0.13M TRIS-Maleate, 0.10M 4.5C5 Na-Formate, 0.60M Na-Citrate, 0.10M 6.5C6 Mg-Sulfate, 0.48M Na-K-Phosphate, 0.10M 6.5C7 Na-Malonate, 2.20M 6 MG7, 0.51%C8 Na-Chloride, 3.07M Mg-Formate, 0.09M Glycerol, 4.61%C9 Na-Malonate, 1.47M 6 HEPES, 0.10M 6.5 MG8, 0.92%C10 Na-Chloride, 3.10M Na-Acetate, 0.10M 6.5C11 Na-Chloride, 3.04M TRIS-HCl, 0.10M 8.5 K-Chloride, 0.04MC12 Na-Malonate, 0.75M 7 Imidazole-Maleate, 0.10M 8.5 LDAO, 0.14%D1 Na-Malonate, 1.45M 7D2 Na-Citrate, 0.24M BisTriPro, 0.10M 8.5 LDAO, 0.73%D3 Na-Malonate, 1.87M 6 TRIS-Maleate, 0.10M 5.5 EDTA, 0.02MD4 Na-Formate, 2.78M TRIS-HCl, 0.10M 8.5 Mg-Chloride, 0.10MD5 Am-Sulfate, 1.46M BisTriPro, 0.10M 5.5 Na-Chloride, 0.36MD6 Am-Phosphate, 0.90M Imidazole-Maleate, 0.10M 6.5D7 Na-Malonate, 0.86M 7 TRIS-HCl, 0.10M 7.5 Na-Acetate, 0.05MD8 Na-K-Phosphate, 1.59M Imidazole-Maleate, 0.10M 7.5D9 Mg-Acetate, 0.33M Imidazole-Maleate, 0.10M 5.5 MG8, 0.26%D10 Na-Malonate, 1.56M 7D11 Na-K-Tartrate, 1.34M Na-Succinate, 0.10M 6.5D12 Na-Malonate, 0.91M 7 Na-Chloride, 0.41M Glycerol, 2.79%E1 Na-Citrate, 1.18M Na-Acetate, 0.10M 4.5E2 Na-Citrate, 0.63M Imidazole-Maleate, 0.10M 6.5 Am-Phosphate, 0.09M LDAO, 0.87%E3 Mg-Formate, 0.27M HEPES, 0.10M 7.5E4 Na-Citrate, 1.01M Na-Succinate, 0.10M 4.5 LDAO, 0.57%E5 Mg-Formate, 0.25M BisTriPro, 0.10M 5.5 K-Chloride, 0.14ME6 Na-Formate, 2.28M Na-Acetate, 0.10M 4.5E7 Na-Formate, 2.43M Imidazole-Maleate, 0.10M 4.5 Mg-Sulfate, 0.10ME8 Na-Malonate, 0.70M 6 Na-K-Phosphate, 0.10M 6.5E9 Na-Acetate, 1.88M Glycerol, 5.39%E10 Am-Sulfate, 0.74M Na-Chloride, 0.21M Glycerol, 3.09%E11 Na-K-Tartrate, 0.95M TRIS-Maleate, 0.10M 6.5 MG8, 0.52%E12 Am-Sulfate, 0.40MF1 Mg-Formate, 0.62M Imidazole-Maleate, 0.10M 5.5 LDAO, 0.86%F2 Na-Malonate, 1.18M 6 BisTriPro, 0.10M 8.5 Ca-Acetate, 0.04MF3 Na-Chloride, 2.93M Am-Phosphate, 0.14MF4 Am-Phosphate, 0.62M Na-K-Phosphate, 0.17MF5 Na-Formate, 2.47M Imidazole-Maleate, 0.10M 5.5 EDTA, 0.01M BOG, 0.41%F6 Na-Chloride, 0.76M EDTA, 0.02M LDAO, 0.14%F7 Na-Formate, 2.12M Na-Citrate, 0.10M 6.5F8 Am-Phosphate, 1.26M Glycerol, 5.91%F9 Na-Chloride, 2.56M Na-K-Phosphate, 0.10M 8.5F10 Na-Malonate, 1.62M 6 MG8, 0.94%F11 Na-Malonate, 0.51M 7 Na-Citrate, 0.10M 5.5 Glycerol, 6.91%F12 Na-Malonate, 2.02M 6G1 Na-Chloride, 0.77M Na-K-Phosphate, 0.10M 8.5 LDAO, 0.69%G2 Na-Chloride, 2.59MG3 Na-Chloride, 1.43M Na-Succinate, 0.10M 5.5 Am-Phosphate, 0.13M Glycerol, 9.93%G4 Na-Malonate, 0.89M 7 Na-K-Phosphate, 0.10M 5.5 EDTA, 0.02M BOG, 0.84%G5 Na-Malonate, 1.28M 7 LDAO, 0.11%G6 Na-Citrate, 1.03M BisTriPro, 0.10M 5.5 Am-Formate, 0.35MG7 Mg-Formate, 0.22MG8 Na-K-Tartrate, 1.26M BisTriPro, 0.10M 6.5 Am-Sulfate, 0.24MG9 Na-Formate, 1.60M Na-Succinate, 0.10M 6.5 Am-Acetate, 0.16M MG7, 0.86%G10 Na-Chloride, 2.77M BisTriPro, 0.10M 7.5 Mg-Chloride, 0.21MG11 Na-K-Tartrate, 1.01M HEPES, 0.10M 7.5G12 Na-Chloride, 1.03M Na-K-Phosphate, 0.10M 7.5 Am-Formate, 0.48MH1 Mg-Formate, 0.68M Na-Acetate, 0.10M 6.5 Ca-Acetate, 0.02MH2 Na-Chloride, 0.81M HEPES, 0.10M 7.5 LDAO, 0.29%H3 Na-Chloride, 1.99M HEPES, 0.10M 7.5 Glycerol, 9.11%H4 Na-Malonate, 1.19M 6 Na-Succinate, 0.10M 6.5H5 Na-Formate, 2.12M Na-Acetate, 0.10M 6.5 LDAO, 0.43%H6 Na-Acetate, 2.54M TRIS-HCl, 0.10M 6.5H7 Mg-Sulfate, 0.66M Na-Citrate, 0.10M 6.5H8 Na-Citrate, 1.12M TRIS-HCl, 0.10M 6.5 Am-Acetate, 0.08MH9 Mg-Sulfate, 0.40M BOG, 0.65%H10 Mg-Acetate, 1.38M Na-Acetate, 0.10M 5.5H11 Am-Sulfate, 0.46M Na-Citrate, 0.10M 4.5H12 Na-Malonate, 0.35M 6 BisTriPro, 0.10M 5.5 MG7, 0.96%

9

Systematical ly Control led Crystal l ization Screen Set 101（CP-CUSTOM-101）Well Salt Buffer pH PrecipitantA01 0.05M Acetate 5.0 1M MalonateA02 0.05M HEPES 7.6 1M MalonateA03 0.05M Acetate 5.0 1M Ammonium SulfateA04 0.05M HEPES 7.6 1M Ammonium SulfateA05 0.05M Acetate 5.0 15% PEG 3350A06 0.05M HEPES 7.6 15% PEG 3350A07 0.05M Acetate 5.0 2M MalonateA08 0.05M HEPES 7.6 2M MalonateA09 0.05M Acetate 5.0 2M Ammonium SulfateA10 0.05M HEPES 7.6 2M Ammonium SulfateA11 0.05M Acetate 5.0 30% PEG 3350A12 0.05M HEPES 7.6 30% PEG 3350B01 0.05M Acetate 5.0 1M Malonate, 20% v/v PEG 400B02 0.05M HEPES 7.6 1M Malonate, 20% v/v PEG 400B03 0.05M Acetate 5.0 1M Ammonium Sulfate, 20% v/v PEG 400B04 0.05M HEPES 7.6 1M Ammonium Sulfate, 20% v/v PEG 400B05 0.05M Acetate 5.0 30% PEG 3350, 20% v/v PEG 400B06 0.05M HEPES 7.6 30% PEG 3350, 20% v/v PEG 400B07 0.01M Zinc Acetate, 0.1M Malonate 0.05M Acetate 5.0 30% v/v PEG 400, 5% DMSOB08 0.01M Magnesium Chloride, 0.1M NaCl 0.05M HEPES 7.6 30% v/v PEG 400, 5% DMSOB09 0.1M Phosphate 0.05M Tris-HCl 8.0 30% v/v PEG 400B10 0.05M Acetate 5.0 0.7M Malonate, 5% w/v PEG 8000B11 0.05M HEPES 7.6 0.7M Malonate, 5% w/v PEG 8000B12 0.05M Acetate 5.0 0.7M Ammonium Sulfate, 5% w/v PEG 8000C01 0.05M HEPES 7.6 0.7M Ammonium Sulfate, 5% w/v PEG 8000C02 0.05M Acetate 5.0 15% PEG 3350, 5% w/v PEG 8000C03 0.05M HEPES 7.6 15% PEG 3350, 5% w/v PEG 8000C04 0.05M Acetate 5.0 0.5M Malonate, 10% w/v PEG 8000C05 0.05M HEPES 7.6 0.5M Malonate, 10% w/v PEG 8000C06 0.05M Acetate 5.0 0.7M Ammonium Sulfate, 10% w/v PEG 8000C07 0.05M HEPES 7.6 0.7M Ammonium Sulfate, 10% w/v PEG 8000C08 0.05M Acetate 5.0 30% PEG 3350, 10% w/v PEG 8000C09 0.05M HEPES 7.6 30% PEG 3350, 10% w/v PEG 8000C10 0.01M Cadmium Acetate, 0.1M Malonate 0.05M Acetate 5.0 20% w/v PEG 8000C11 0.1M Phosphate 0.05M MES 5.5 20% w/v PEG 8000C12 0.1M Ammonium Sulfate 0.05M HEPES 7.6 20% w/v PEG 8000, 5% DMSOD01 0.05M Acetate 5.0 1M Malonate, 0.4M PhosphateD02 0.05M HEPES 7.6 1M Malonate, 0.4M PhosphateD03 0.05M Acetate 5.0 1M Ammonium Sulfate, 0.4M PhosphateD04 0.05M HEPES 7.6 1M Ammonium Sulfate, 0.4M PhosphateD05 0.05M Acetate 5.0 15% PEG 3350, 0.4M PhosphateD06 0.05M HEPES 7.6 15% PEG 3350, 0.4M PhosphateD07 0.05M HEPES 7.6 0.9M Phosphate, 2% 2-propanolD08 0.05M Tris-HCl 8.0 0.9M Phosphate, 2% PEG 400, 5% DMSOD09 0.05M Acetate 5.0 1M Malonate, 5% v/v MPDD10 0.05M HEPES 7.6 1M Malonate, 5% v/v MPDD11 0.05M Acetate 5.0 1M Ammonium Sulfate, 5% v/v MPDD12 0.05M HEPES 7.6 1M Ammonium Sulfate, 5% v/v MPDE01 0.05M Acetate 5.0 15% PEG 3350, 5% v/v MPDE02 0.05M HEPES 7.6 15% PEG 3350, 5% v/v MPDE03 0.05M Acetate 5.0 1M Malonate, 10% v/v MPDE04 0.05M HEPES 7.6 1M Malonate, 10% v/v MPDE05 0.05M Acetate 5.0 1M Ammonium Sulfate, 10% v/v MPDE06 0.05M HEPES 7.6 1M Ammonium Sulfate, 10% v/v MPDE07 0.05M Acetate 5.0 30% PEG 3350, 10% v/v MPDE08 0.05M HEPES 7.6 30% PEG 3350, 10% v/v MPDE09 0.01M Calcium Chloride, 0.1M NaCl 0.05M Acetate 5.0 30% v/v MPDE10 0.05M Acetate 5.0 30% v/v MPD, 5% DMSOE11 0.01M Zinc Acetate 0.05M MES 5.5 30% v/v MPDE12 0.1M Ammonium Sulfate, 0.01M Cadmium Acetate 0.05M MES 5.5 30% v/v MPD, 5% DMSOF01 0.05M Acetate 5.0 1M Malonate, 15% v/v 2-propanolF02 0.05M HEPES 7.6 1M Malonate, 15% v/v 2-propanolF03 0.05M Acetate 5.0 1M Ammonium Sulfate, 15% v/v 2-propanolF04 0.05M HEPES 7.6 1M Ammonium Sulfate, 15% v/v 2-propanolF05 0.05M Acetate 5.0 15% PEG 3350, 15% v/v 2-propanolF06 0.05M HEPES 7.6 15% PEG 3350, 15% v/v 2-propanolF07 0.05M Acetate 5.0 2M Malonate, 15% v/v 2-propanolF08 0.05M HEPES 7.6 2M Malonate, 15% v/v 2-propanolF09 0.05M Acetate 5.0 2M Ammonium Sulfate, 15% v/v 2-propanolF10 0.05M HEPES 7.6 2M Ammonium Sulfate, 15% v/v 2-propanolF11 0.05M Acetate 5.0 30% PEG 3350, 15% v/v 2-propanolF12 0.05M HEPES 7.6 30% PEG 3350, 15% v/v 2-propanolG01 0.1M Malonate 0.05M MES 5.5 20% v/v 2-propanolG02 0.01M Calcium Chloride 0.05M Tris-HCl 8.0 20% v/v 2-propanolG03 0.05M Acetate 5.0 1M Malonate, 1M NaCIG04 0.05M HEPES 7.6 1M Malonate, 1M NaCIG05 0.05M Acetate 5.0 1M Ammonium Sulfate, 1M NaCIG06 0.05M HEPES 7.6 1M Ammonium Sulfate, 1M NaCIG07 0.05M Acetate 5.0 15% PEG 3350, 1M NaCIG08 0.05M HEPES 7.6 15% PEG 3350, 1M NaCIG09 0.05M Acetate 5.0 2M Malonate, 1M NaCIG10 0.05M HEPES 7.6 2M Malonate, 1M NaCIG11 0.05M Acetate 5.0 2M Ammonium Sulfate, 1M NaCIG12 0.05M HEPES 7.6 2M Ammonium Sulfate, 1M NaCIH01 0.05M Acetate 5.0 30% PEG 3350, 1M NaCIH02 0.05M HEPES 7.6 30% PEG 3350, 1M NaCIH03 0.05M Acetate 5.0 2M NaClH04 0.01M Manganese Chloride 0.05M MES 5.5 2M NaClH05 0.01M Magnesium Chloride 0.05M Tris-HCl 8.0 2M NaCl, 2% MPD, 5% DMSOH06 0.01M Magnesium Chloride 0.05M Acetate 5.0 1.8M Ammonium Sulfate, 2% 2-propanol, 5% DMSOH07 0.01M Manganese Chloride 0.05M MES 5.5 1.8M Ammonium Sulfate, 2% 2-propanol, 5% DMSOH08 0.01M Magnesium Chloride 0.05M HEPES 7.6 1.8M Ammonium Sulfate, 5% DMSOH09 0.01M Magnesium Chloride 0.05M MES 5.5 1M malonate, 2% PEG 400H10 0.01M Manganese Chloride 0.05M MES 5.5 1M malonate, 2% MPD, 5% DMSOH11 0.01M Calcium Chloride 0.05M HEPES 7.6 1M malonate, 2% MPDH12 0.01M Cadmium Acetate 0.05M HEPES 7.6 1M malonate, 2% PEG 400, 5% DMSO

10

Sparse Matrix Screen set I （CP-CUSTOM-102）Well Salt Buffer pH Precipitant 1 Precipitant 2

1 Ca-Chloride, 0.02M Na-Acetate, 0.1M 4.6 MPD, 30% (%v/v)2 Na-K-Tartrate, 0.4M3 Am-Phosphate, 0.4M4 Tris-HCl, 0.1M 8.5 Am-Sulfate, 2M5 Na-Citrate, 0.2M HEPES-Na, 0.1M 7.5 MPD, 40% (%v/v)6 Mg-Chloride, 0.2M Tris-HCl, 0.1M 8.5 PEG 4,000, 30% (%w/v)7 Na-Cacodylate, 0.1M 6.5 Na-Acetate, 1.4M 8 Na-Citrate, 0.2M Na-Cacodylate, 0.1M 6.5 Isopropanol, 30% (%v/v)9 Am-Acetate, 0.2M Na-Citrate, 0.1M 5.6 PEG 4,000, 30% (%w/v)10 Am-Acetate, 0.2M Na-Acetate, 0.1M 4.6 PEG 4,000, 30% (%w/v)11 Na-Citrate, 0.1M 5.6 Am-Phosphate, 1M12 Mg-Chloride, 0.2M HEPES-Na, 0.1M 7.5 Isopropanol, 30% (%v/v)13 Na-Citrate, 0.2M Tris-HCl, 0.1M 8.5 PEG 400, 30% (%v/v)14 Ca-Chloride, 0.2M HEPES-Na, 0.1M 7.5 PEG 400, 28% (%v/v)15 Am-Sulfate, 0.2M Na-Cacodylate, 0.1M 6.5 PEG 8,000, 30% (%w/v)16 HEPES-Na, 0.1M 7.5 Li-Sulfate, 1.5M17 Li-Sulfate, 0.2M Tris-HCl, 0.1M 8.5 PEG 4,000, 30% (%w/v)18 Mg-Acetate, 0.2M Na-Cacodylate, 0.1M 6.5 PEG 8,000, 20% (%w/v)19 Am-Acetate, 0.2M Tris-HCl, 0.1M 8.5 Isopropanol, 30% (%v/v)20 Am-Sulfate, 0.2M Na-Acetate, 0.1M 4.6 PEG 4,000, 25% (%w/v)21 Mg-Acetate, 0.2M Na-Cacodylate, 0.1M 6.5 MPD, 30% (%v/v)22 Na-Citrate, 0.2M Tris-HCl, 0.1M 8.5 PEG 4,000, 30% (%w/v)23 Mg-Chloride, 0.2M HEPES-Na, 0.1M 7.5 PEG 400, 30% (%v/v)24 Ca-Chloride, 0.2M Na-Acetate, 0.1M 4.6 Isopropanol, 20% (%v/v)25 Imidazole 6.5 Na-Acetate, 1.0M26 Am-Acetate, 0.2M Na-Citrate, 0.1M 5.6 MPD, 30% (%v/v)27 Na-Citrate, 0.2M HEPES-Na, 0.1M 7.5 Isopropanol, 20% (%v/v)28 Na-Acetate, 0.2M Na-Cacodylate, 0.1M 6.5 PEG 8,000, 30% (%w/v)29 HEPES-Na, 0.1M 7.5 Na-K-Tartrate, 0.8M30 Am-Sulfate, 0.2M PEG 8,000, 30% (%w/v)31 Am-Sulfate, 0.2M PEG 4,000, 30% (%w/v)32 Am-Sulfate, 2M33 Na-Formate, 4M34 Na-Acetate, 0.1M 4.6 Na-Formate, 2M35 HEPES-Na, 0.1M 7.5 Na-K-Phosphate, 1.6M 36 Tris-HCl, 0.1M 8.5 PEG 8,000, 8% (%w/v)37 Na-Acetate, 0.1M 4.6 PEG 4,000, 8% (%w/v)38 HEPES-Na, 0.1M 7.5 Na-Citrate, 1.4M39 HEPES-Na, 0.1M 7.5 PEG 400, 2% (%v/v) Am-Sulfate, 2.0M40 Na-Citrate, 0.1M 5.6 Isopropanol, 20% (%v/v) PEG 4,000, 20% (%w/v)41 HEPES-Na, 0.1M 7.5 Isopropanol, 10% (%v/v) PEG 4,000, 20% (%w/v)42 K-Phosphate, 0.05M PEG 8,000, 20% (%w/v)43 PEG 1500, 30% (%w/v)44 Mg-Formate, 0.2M 45 Zn-Acetate, 0.2M Na-Cacodylate, 0.1M 6.5 PEG 8,000, 18% (%w/v)46 Ca-Acetate, 0.2M Na-Cacodylate, 0.1M 6.5 PEG 8,000, 18% (%w/v)47 Na-Acetate, 0.1M 4.6 Am-Sulfate, 2.0M48 Tris-HCl, 0.1M 8.5 Am-Sulfate, 2.0M 49 Li-Sulfate, 1.0M PEG 8,000, 2% (%w/v)50 Li-Sulfate, 1.0M PEG 8,000, 15% (%w/v)

Sparse Matrix Screen set Ⅱ （CP-CUSTOM-103）Well Salt Buffer pH Precipitant 1 Precipitant 2

1 Na-Chloride, 2.0M PEG 6,000, 10% (%w/v)2 Na-Chloride, 0.5M, Mg-Chloride, 0.01M CTAB, 0.01M3 Ethylene glycol, 25% (%v/v)4 Dioxane, 35% (%v/v) 5 Am-Sulfate, 2.0M Isopropanol, 5% (%v/v) 6 7.0 Imidazole, 1M7 PEG 1,000, 10% (%w/v) PEG 8,000, 10% (%w/v)8 Na-Chloride, 1.5M Ethanol, 10% (%v/v)9 Na-Acetate, 0.1M 4.6 Na-Chloride, 2M10 Na-Chloride, 0.2M Na-Acetate, 0.1M 4.6 MPD, 30% (%v/v)11 Co-Chloride, 0.01M Na-Acetate, 0.1M 4.6 Hexanediol, 1M12 Cd-Chloride, 0.1M Na-Acetate, 0.1M 4.6 PEG 400, 30% (%v/v)13 Am-Sulfate, 0.2M Na-Acetate, 0.1M 4.6 PEG-MME 2,000, 30% (%w/v)14 Na-K-Tartrate, 0.2M Na-Citrate, 0.1M 5.6 Am-Sulfate, 2M 15 Am-Sulfate, 0.5M Na-Citrate, 0.1M 5.6 Li-Sulfate, 1M 16 Na-Chloride, 0.5M Na-Citrate, 0.1M 5.6 Polyethylene imine, 2% (%v/v) 17 Na-Citrate, 0.1M 5.6 2-Butanol, 35% (%v/v) 18 Fe-Chloride, 0.01M Na-Citrate, 0.1M 5.6 Jeffamine M-600, 10% (%v/v) 19 Na-Citrate, 0.1M 5.6 Hexanediol, 2.5M20 MES, 0.1M 6.5 Mg-Sulfate, 1.6M 21 Na-K-Phosphate, 0.2M MES, 0.1M 6.5 Na-Chloride, 2.0M22 MES, 0.1M 6.5 PEG 20,000, 12% (%v/v) 23 Am-Sulfate, 1.6M MES, 0.1M 6.5 Dioxane, 10% (%v/v) 24 Cs-chloride, 0.05M MES, 0.1M 6.5 Jeffamine M-600, 30% (%v/v) 25 Co-Chloride, 0.01M MES, 0.1M 6.5 Am-Sulfate, 1.8M 26 Am-Sulfate, 0.2M MES, 0.1M 6.5 PEG-MME 5,000, 30% (%w/v) 27 Zn-Sulfate, 0.01M MES, 0.1M 6.5 PEG-MME 550, 25% (%v/v) 28 Na-Citrate, 1.6M 29 Am-Sulfate, 0.5M HEPES, 0.1M 7.5 MPD, 30% (%v/v)30 HEPES, 0.1M 7.5 PEG 6,000, 10% (%w/v) MPD, 5% (%v/v)31 HEPES, 0.1M 7.5 Jeffamine M-600, 20% (%v/v) 32 Na-Chloride, 0.1M HEPES, 0.1M 7.5 Am-Sulfate, 1.6M 33 HEPES, 0.1M 7.5 Am-Formate, 2.0M34 Cd-Sulfate, 0.05M HEPES, 0.1M 7.5 Na-Acetate, 1.0M 35 HEPES, 0.1M 7.5 MPD, 70% (%v/v) 36 HEPES, 0.1M 7.5 Na-Chloride, 4.3M37 HEPES, 0.1M 7.5 PEG 8,000, 10% (%w/v) Ethylene glycol, 8% (%v/v)38 HEPES, 0.1M 7.5 PEG 10,000, 20% (%w/v) 39 Mg-Chloride, 0.2M Tris-HCl, 0.1M 8.5 Hexanediol, 3.4M40 Tris-HCl, 0.1M 8.5 2-Butanol, 25% (%v/v) 41 Ni-Chloride, 0.01M Tris-HCl, 0.1M 8.5 Li-Sulfate, 1.0M 42 Am-sulfate, 1.5M Tris-HCl, 0.1M 8.5 Glycerol, 12% (%v/v) 43 Am-Phosphate, 0.2M Tris-HCl, 0.1M 8.5 MPD, 50% (%v/v) 44 Tris-HCl, 0.1M 8.5 Ethanol, 20% (%v/v)45 Ni-Chloride, 0.01M Tris-HCl, 0.1M 8.5 PEG-MME 2,000, 20% (%w/v)46 Na-Chloride, 0.1M Bicine, 0.1M 9.0 PEG-MME 550, 20% (%v/v) 47 Bicine, 0.1M 9.0 Mg-Chloride, 2.0M 48 Bicine, 0.1M 9.0 Dioxane, 2% (%v/v) PEG 20,000, 10% (%w/v)

11

器材AxyGem Crystallography Plate (CP-AxyGem-96-50)

◆ AxyPearl Lid （CP-AxyPearl-192-100)

ご注意：本製品は上部のリッド（蓋）のみです。写真上の下側のプレートは含まれませんのでご注意下さい。

高純度ポリスチレン： 偏光を抑制した高光透過性の高純度ポリスチレン製帯電防止プラスチック包装： 静電気の影響を防止D型試薬ウェル形状： 結晶の光学検査を妨げない丸底のサブウェル： 蛋白試料と試薬が均一に混ざりやすく、且つ、結晶を壊さずに掻き出し易い洗浄スペース：プレート上の結晶洗浄スペースにより、結晶を壊す可能性を低減サブウェル容量： 4.3 uL / Bufferウェル容量： 250 uLです。SBS規格準拠有機溶媒耐性スタッキング可能バーコードラベル：オプションで添付可能

192点のハンギングドロップ可能AxyGem Crystallography Plate （CP-AXYGEM-96）と組み合わせ、192のハンギングドロップと96のシッティングドロップを同時に実験可能

高純度ポリスチレン： 偏光を抑制した高光透過性の高純度ポリスチレン製エンボス状のガイド格子： 視覚的に結晶を見つけ易い

A1-H12表示によりウェル位置の確認が容易

•帯電防止プラスチック包装： 静電気の影響を防止•SBS規格準拠

• 有機溶媒耐性• バーコードラベル：オプションで添付可能

結晶洗浄スペース

シッティングドロップ用タンパク質結晶化プレートです。

ハンギングドロップ用リッドです。

コードNo. メーカーコード 品名 容量希望納入価格

（円)

511-51991 CP-AxyGem-96-50 AxyGem Crystallography Plate 50plates 75,000

コードNo. メーカーコード 品名 容量希望納入価格

（円)

518-52001 CP-AxyPearl-192-100 AxyPearl Lid for High-throughput Hanging Drop 100lids 81,300

12

ご注意： カバーガラスやシーラントは含まれまれておりません。

UltraClear Pressure Sensitive Sealing Film

CrystalPlate-24, -48 (CP-CrystalPlate-24, 48)

高純度ポリスチレン製

底面：フラット

蓋付き

24穴には18ミリ、48穴には12ミリの円形あるいは角型のカバースライドが対応

スタッキング可能

有機溶媒耐性

CrystalPlate-24•24ウェルプレート•プレート寸法: 12.5 cm x 8.3 cm x 2.0 cm •ウェル寸法: 1.7 cm x 1.7 cm for the 24-well •ウェル許容量: 3.5 ml•ウェル使用推奨容量: 100µl

CrystalPlate-48•48ウェルプレート•プレート寸法: 12.5 cm x 8.3 cm x 2.0 cm •ウェル寸法: 1.1 cm x 1.1 cm •ウェル許容量: 1.5 ml •ウェル使用推奨容量: 100-300 µl

ハンギングドロップやシッティングドロップ蒸気拡散法によるタンパク質結晶化に最適な24ウェルと48ウェルの

プレートです。

非常に優れた光透過性材質：ポリエステルポリカーボネート、ポリプロピレン及びポリスチレンプレートに対応サンプルの蒸発を抑制加圧により接着（粘着式ではありません。）プレカットタイプ： 96穴や384穴のプレートサイズ手動、あるいは自動シーラーで容易にシール可能取扱が簡単： シールの一端は容易に台紙から剥離できる構造であり、結晶観測面への指紋の付着を防止使用可能温度範囲：-80℃～110℃有機溶媒耐性

光透過性に非常優れた感圧接着プレートシールです。結晶プレートの観察に最適です。また、PCRプレートに

もご利用頂くことができます。

UltraClear™シール 一般的な透明シール

コードNo. メーカーコード 品名 容量希望納入価格

（円)

515-52011 CP-CrystalPlate-24 24 well Crystallography Plates with lid 50plates 21,900

512-52021 CP-CrystalPlate-48 48 well Crystallography Plates with Lid 50plates 21,900

コードNo. メーカーコード 品名 容量希望納入価格

（円)

519-52031 UC-500 Ultra Clear Pressure Sensitive Sealing Film 500Sheets 154,500

13

結晶化試薬

◆ バッファー

◆ 界面活性剤

コードNo. メーカーコード 品名 容量希望納入価格

(円）- CP-Buffer-1 1.0 M BisTriPro, pH 5.5, 100ml in a bottle 100ml 24,000- CP-Buffer-2 1.0 M BisTriPro, pH 6.5, 100ml in a bottle 100ml 24,000- CP-Buffer-3 1.0 M BisTriPro, pH 7.5, 100ml in a bottle 100ml 24,000- CP-Buffer-4 1.0 M BisTriPro, pH 8.5, 100ml in a bottle 100ml 24,000- CP-Buffer-5 3.40 M Na-Malonate pH 4, 200ml in a bottle 200ml 17,500- CP-Buffer-6 3.40 M Na-Malonate pH 5, 200ml in a bottle 200ml 17,500- CP-Buffer-7 3.40 M Na-Malonate pH 6, 200ml in a bottle 200ml 17,500- CP-Buffer-8 3.40 M Na-Malonate pH 7, 200ml in a bottle 200ml 17,500- CP-Buffer-9 1.0 M HEPES, pH 6.5, 100ml in a bottle 100ml 20,000- CP-Buffer-10 1.0 M HEPES, pH 7.5, 100ml in a bottle 100ml 20,000- CP-Buffer-11 1.0 M HEPES, pH 8.5, 100ml in a bottle 100ml 20,000- CP-Buffer-12 1.0 M Na-Succinate, pH 4.5, 200ml in a bottle 200ml 20,000- CP-Buffer-13 1.0 M Na-Succinate, pH 5.5, 200ml in a bottle 200ml 20,000- CP-Buffer-14 1.0 M Na-Succinate, pH 6.5, 200ml in a bottle 200ml 20,000- CP-Buffer-15 1.0 M Na-Succinate, pH 7.5, 200ml in a bottle 200ml 20,000- CP-Buffer-16 1.0 M Na-Citrate, pH 4.5, 100ml in a bottle 100ml 5,000- CP-Buffer-17 1.0 M Na-Citrate, pH 5.5, 100ml in a bottle 100ml 5,000- CP-Buffer-18 1.0 M Na-Citrate, pH 6.5, 100ml in a bottle 100ml 5,000- CP-Buffer-19 1.0 M Na-Citrate, pH 7.5, 100ml in a bottle 100ml 5,000- CP-Buffer-20 1.0 M MES, pH 5.5, 100ml in a bottle 100ml 25,000- CP-Buffer-21 1.0 M MES, pH 6.5, 100ml in a bottle 100ml 25,000- CP-Buffer-22 1.0 M MOPS, pH 6.5, 200ml in a bottle 200ml 30,000- CP-Buffer-23 1.0 M MOPS, pH 7.5, 200ml in a bottle 200ml 30,000- CP-Buffer-24 1.0 M TRIS-Maleate, pH 4.5, 200ml in a bottle 200ml 8,000- CP-Buffer-25 1.0 M TRIS-Maleate, pH 5.5, 200ml in a bottle 200ml 8,000- CP-Buffer-26 1.0 M TRIS-Maleate, pH 6.5, 200ml in a bottle 200ml 8,000- CP-Buffer-27 1.0 M TRIS-HCl, pH 6.5, 100ml in a bottle 100ml 7,000- CP-Buffer-28 1.0 M TRIS-HCl, pH 7.5, 100ml in a bottle 100ml 7,000- CP-Buffer-29 1.0 M TRIS-HCl, pH 8.0, 100ml in a bottle 100ml 7,000- CP-Buffer-30 1.0 M TRIS-HCl, pH 8.5, 100ml in a bottle 100ml 7,000- CP-Buffer-31 1.0 M Na-Acetate, pH 4.5, 100ml in a bottle 100ml 4,000- CP-Buffer-32 1.0 M Na-Acetate, pH 5.0, 100ml in a bottle 100ml 4,500- CP-Buffer-33 1.0 M Na-Acetate, pH 5.5, 100ml in a bottle 100ml 4,500- CP-Buffer-34 1.0 M Na-Acetate, pH 6.5, 100ml in a bottle 100ml 4,500- CP-Buffer-35 1.0 M Imidazole-Maleate, pH 4.5, 100ml in a bottle 100ml 13,000- CP-Buffer-36 1.0 M Imidazole-Maleate, pH 5.5, 100ml in a bottle 100ml 13,000- CP-Buffer-37 1.0 M Imidazole-Maleate, pH 6.5, 100ml in a bottle 100ml 13,000- CP-Buffer-38 1.0 M Imidazole-Maleate, pH 7.5, 100ml in a bottle 100ml 13,000- CP-Buffer-39 1.0 M Imidazole-Maleate, pH 8.5, 100ml in a bottle 100ml 13,000- CP-Buffer-40 1.0 M Na-K-Phosphate, pH 5.5, 200ml in a bottle 200ml 12,000- CP-Buffer-41 1.0 M Na-K-Phosphate, pH 6.5, 200ml in a bottle 200ml 12,000- CP-Buffer-42 1.0 M Na-K-Phosphate, pH 7.5, 200ml in a bottle 200ml 12,000- CP-Buffer-43 1.0 M Na-K-Phosphate, pH 8.5, 200ml in a bottle 200ml 12,000

コードNo. メーカーコード 品名 容量希望納入価格

(円）

- CP-Det-A 10% MG7, 1 ml in a tube 1 ml 11,000

- CP-Det-B 10% MG8, 1 ml in a tube 1 ml 12,400

- CP-Det-C 10% LDAO, 10 ml in a tube 10 ml 5,000

- CP-Det-D 10% BOG, 1 ml in a tube 1 ml 16,000

- CP-Det-E 10% SBOG, 1 ml in a tube 1 ml 18,000

14

0806

希望納入価格は「本体価格のみ」で消費税等は含まれておりません。

掲載商品は主として試験・研究用の目的にのみに使用される「試薬」です。「試薬」は「医薬品」、「食品」、「家庭用品」などとしては使用できません。

◆ 沈殿剤、塩、添加剤

コードNo. メーカーコード 品名 容量希望納入価格

(円）

- CP-Pre/Adi-55 1M Ca-Acetate, 200ml in a bottle 200ml 13,000

- CP-Pre/Adi-56 3.6M Ammonium-Sulfate, 200ml in a bottle 200ml 12,000

- CP-Pre/Adi-57 2.0M Ammonium-Phosphate, 200ml in a bottle 200ml 12,000

- CP-Pre/Adi-58 4.0M Ammonium-Acetate, 200ml in a bottle 200ml 8,000

- CP-Pre/Adi-59 10M Ammonium-Formate, 200ml in a bottle 200ml 25,000

- CP-Pre/Adi-60 2.0M Na-K-Tartrate, 200ml in a bottle 200ml 13,000

- CP-Pre/Adi-61 4.0M Na-K-Phosphate, 200ml in a bottle 200ml 17,000

- CP-Pre/Adi-62 4.0M Na-Acetate, 200ml in a bottle 200ml 12,000

- CP-Pre/Adi-63 4.0M Na-Formate, 200ml in a bottle 200ml 13,000

- CP-Pre/Adi-64 1.6M Na-Citrate, 200ml in a bottle 200ml 13,000

- CP-Pre/Adi-65 5.0 M Nacl, 200ml in a bottle 200ml 5,000

- CP-Pre/Adi-66 3.5M K-Chloride, 200ml in a bottle 200ml 17,000

- CP-Pre/Adi-67 1.0M Magnesium-Formate, 200ml in a bottle 200ml 17,000

- CP-Pre/Adi-68 2.0M Magnesium Sulfate, 200ml in a bottle 200ml 15,000

- CP-Pre/Adi-69 2.0M Mg-Acetate, 100ml in a bottle 100ml 13,000

- CP-Pre/Adi-70 0.5M Mn-Chloride, 100ml in a bottle 100ml 10,000

- CP-Pre/Adi-71 2.0M Lithium-Sulfate, 200ml in a bottle 200ml 19,000

- CP-Pre/Adi-72 80% Glycerol, 100ml in a bottle 100ml 7,000

- CP-Pre/Adi-74 0.5M EDTA, 100ml in a bottle 100ml 10,000

- CP-Pre/Adi-75 1M Zinc-Acetate, 100ml in a bottle 100ml 12,000

- CP-Pre/Adi-76 4.0M Magnesium-Chloride, 200ml in a bottle 200ml 15,000

- CP-Pre/Adi-77 2.5M Ca-Chloride, 100ml in a bottle 100ml 13,000

- CP-Pre/Adi-78 1.0M Cd-Acetate, 100ml in a bottle 100ml 15,000

- CP-Pre/Adi-80 60% 1,6-Hexanediol, 200ml in a bottle 200ml 10,000