Compendium - Department of Biotechnology · This compendium has been distributed to different parts of the country among the researchers and was found to be useful to the scientists

Compiled by

Dr. A. S. Ninawe, Adviser, Department of Biotechnology

& Dr.A.S.Sahul Hameed,

OIE Expert, C.Abdul Hakeem College, Melvisharam, Vellore Dt. TN

Compendium

Outcome of DBT – Sponsored Projects in Aquaculture and Marine Biotechnology Taskforce

(2010 – 2014)

Compiled by

Dr.A.S.Ninawe, Adviser&

Dr.S.U.Ahmed, Scientist ‘D’Department of Biotechnology

andDr.A.S.Sahul Hameed, OIE Expert,

C.Abdul Hakeem College,Melvisharam, Vellore Dt, TN

Department of Biotechnology Ministry of Science and Technology, Government of India

2015

F O R e W O R D

It has been a good experience to go through the first Compendium on the outcome of R & D Projects in Aquaculture and Marine Biotechnology supported by DBT through the Task Force Mechanism during the period from 1998 to 2010. Taking the journey forward since 2010 onwards, I am delighted to see the progress in Marine Biology and Biotechnology sector which is in a large measure due to the efforts of the Committee Members. The priorities of research have been well conceptualized and good proposals formulated addressing the country’s need for sustainable and judicious utilization of its long coast line for production of high value products like nutraceuticals, marine drugs and chemicals, novel enzymes and proteins. Efforts have been made to utilize the research leads for commercialization of technologies and product and process development. The DBT has extended support to about 300 scientists for various research projects, the outcome of which could be about 205 publications in nationally and internationally reputed journals. Half-a-dozen patents have been filed and a few more are in the process of being filed. More than 30 PhDs have been produced. Even the staffs engaged in various projects have been employed in Aquaculture and Marine Biology sectors to work on various aspects through R & D.

The outcome of DBT funded projects has been brought out as a compendium with publications, technologies developed and commercialized, and innovations patented. It is worth mentioning that some of these technologies have already been launched in the market and generating good income.

I am sure that this Compendium would be very useful for the scientists, researchers and entrepreneurs working in the sector. I personally compliment the programme division for bringing out the same.

Prof. K. VijayRaghavan secretary Department of biotechnology June, 2015

CONTeNTs

1. Introduction 1

2. Publications 3

3. Patents 26

4. Research Leads Obtained 27

5. Ph. D. Degree Awarded 32

6. Placements 36

7. Products 38

Outcome of DBT sponsored Projects in Aquaculture and Marine Biotechnology Taskforce (2010-2014) 1

INTRODUCTION

Aquaculture and Marine Biotechnology have been the subject of great

importance not only because of the sustainable utilization of their resources to feed the billion people of the world but also for the future challenges for discovery of new products and process development of economic importance through its treasure recognition and diversification. Apart from contributing to high quality and healthy food (aquaculture), nutraceuticals and medicinal products (anti-cancer and antimicrobials), this sector is expected to contribute to sustainable alternative source of energy (biofuel from microalgae) and environmental health. The potential for the contribution of Marine Biotechnology is, therefore, even more relevant now than it was ten years ago and a sound strategy for its development in India is urgently needed to allow this potential to be realized. The Department of Biotechnology (DBT) has been instrumental in promoting marine science and aquaculture under the programme area of Aquaculture and Marine Biotechnology, for more than two decades now. A Taskforce was established for this purpose to fund projects in the priority areas identified in R & D and till today more than 300 projects have been supported to carry out the research in multi-deciplanry areas on detection and prevention of aquatic animal diseases, development of fish and shellfish cell lines, immunostimulants, bioactive molecules (including antiplasmodial molecule), fish feed and nutrition, bioadhesive, biosurfactants, biofuel from marine resources, probiotics, human therapeutic compounds from marine resource etc. To publicise the achievements in this sector, the department brought out the first compendium in 2011 covering research achievements from 1998 till 2010, the first of its kind for the benefit of marine scientists and researchers working in this area and to promote DBT activities through research funding opportunities. The remarkable outcomes are due to the dedicated and strenuous efforts of the Principal Investigators and Co-Investigators with the mentorship of scientists and the coordinators working in an advisory capacity. The outcome of DBT funded projects is the availability of sensitive diagnostic tools such as PCR kit for shrimp viruses including WSSV and RT-PCR for fish nodavirus. Some of these kits are in good demand in aquaculture industry. Vaccines have been developed against fish viral (fish nodavirus) and bacterial pathogens (Aeromonas hydrophila, Edwardsiella tarda and Vibrio anguillarum) under Indo-Norwegian programme during the period from 2010 to 2014. Viral, bacterial and herbal immunostimulants have been formulated to control WSSV and V. harveyii in the culture systems. The recent RNAi technology has been adopted to control WSSV infection in shrimp. Field trials are now being carried out to validate the efficacy of this technique under SIBRI scheme in collaboration with GPS Biotech, Hyderabad.

Our first compendium on the research outcome of DBT sponsored projects sanctioned during the period from 1998 to 2010 covering publications, patents and technology transfer etc was compiled and published in 2011. Out of seven

A haul of farmed major carp

Women in shrimp culture

Researchers of Norway visiting a shrimp hatchery

Outcome of DBT sponsored Projects in Aquaculture and Marine Biotechnology Taskforce (2010-2014)2

patents filed for product/process development two have been granted. Seven technologies have been successfully transferred to industry for commercialization. This compendium has been distributed to different parts of the country among the researchers and was found to be useful to the scientists to identify the grey areas to strengthen the research in the field of aquaculture and marine biotechnology.

Attempt has been made to develop fish cell lines from a variety of fish. Cell line program was initiated with single fish species and subsequently it led to the formation of a network programme involving many research organizations to develop more number of cell lines from a variety of fish during the period from 2010 to 2014. As a result, more than fifty cell lines from a variety of fish have been developed and made available to the scientific community through DBT funding. A Fish Cell Line Repository Facility has been established in NBFGR, Lucknow to hold all these cell lines for scientific research.

A bioreactor has been designed for Re-circulating Aquaculture Systems (RAS) to help to remove organic matter and ammonia by the nitrifying bacterial consortia immobilized on the cartridges in culture systems. Bioremediation technique developed to adopt zero water exchange in shrimp culture system, has been commercialized for multiplying the device for large scale field trials. Molecular signal intervention approach has been developed to induce the maturation in shrimp to avoid the existing technique of eye stalk ablation.

Biocomposite of chitosan and fucoidan isolated from shrimp shell waste and marine algae was prepared in varying compositions and the wound healing ability of the biocomposite film/bandage was evaluated. A shrimp waste based biocomposite gel/ointment was found to be more promising than standard ointments for burn wound healing in experimental animals. This was prepared in the form of a collagen-chitosan film from the extracted collagen and shrimp shell chitosan along with drugs for wound healing property incorporated in the film. The trials have shown promising result and steps are being initiated for commercialization. Gelatin based edible film was developed from queen fish and grouper skin gelatin. The film prepared is transparent, strong and had good barrier and digestibility properties.

Network programmes have been sanctioned during this period to explore bioactive molecules from marine actinobacteria, complete genome analysis of the important freshwater aquaculture fish Labeo rohita (Rohu, a carp) and Clarias batrachus (walking catfish). New programme on National Training Programme in molecular biology and biotechnology for fisheries professionals and a programme on fish reproduction addressing sex manipulation aspects in common carp through endocrinological approaches have been additions to the basket of marine biotechnology programme.

In continuation of our efforts, attempt has been made to compile the outcome of the DBT-funded projects sanctioned during the period from 2011 to 2014 and present the details in the form of publications, patents, research leads, Ph.D. awarded, placements and products in the market. The details of research leads, Ph.D. awarded, products in the market and placements have been included to add more value to this compendium.


PUblICATIONs

One of the important outcomes of DBT funded R & D projects is publications in well reputed journals. The total number of publications contributed by 86 principal investigators (PI) under Aquaculture and Marine Biotechnology Taskforce is 205 during the period from 2011 to 2014. Among the 86 PIs, 28 PIs have not published any research articles. The number of publications during the period from 2011 to 2014 increased to 205 from 180 during the period from 1998 to 2010. Among 205 publications, 166 are International and the remaining are National publications. The number of publications in 2011 was 37 and it gradually increased to 49 in 2012, 46 in 2013 and 73 in 2014 (Fig. 1). The Impact Factor for these publications ranged from 0.313 (Indian Journal of Marine Sciences) to 8.057 (PLOS Pathogens). Dr.S.Mazumder has published one research article in the journal PLOS pathogens which has the highest impact factor among 205 publications (Banerjee et al., 2014). The number of publications with impact factor of less than 1, 2, 3, 4, 5 and above 5 is 27, 53, 39, 28, 07 and 09, respectively (Fig. 2). The number of publications published in the journals without impact factor is 42. The number of PIs with publications of less than 5, 10, and more than 10 is 48, 8 and 2, respectively (Fig. 3). The number of PIs of projects with no publications is 28. Dr.A.S.Sahul Hameed has published 20 papers followed by Dr.Indrani Karunasagar (19) during the period from 2011 to 2014 from DBT funded R & D projects. Among 374 Indian authors who contributed publications in the Aquaculture journal during the period from 1972 to 2011, Dr.A. S. Sahul Hameed scored first rank with 27 publications, followed by Indrani Karunasagar who scored second rank with 20 publications (Ramasamy et al., 2014).

1. Total of No. of Publications : 205

2. No. of National Publications : 039

3. No. of International Publications : 166

4. Average No. of Publications per PI : 2.38

Fig. 1 Year-wise distribution of publications


Fig. 2 Publications with Impact Factor

Principal Investigators with Number of Publications

No. of Publications No. of PI

0 - 28

1- 5 - 48

6 - 10 - 08

11 - 15 - Nil

16 - 20 - 02

Fig. 3 Investigators with number of publications

Publication with Impact Factor

Impact factor No. of Publications

Journals without impact factor - 42

< 1 - 27

1 – 2 - 53

2 - 3 - 39

3 - 4 - 28

4 - 5 - 07

> 5 - 09


Publications from DbT-sponsored Projects under Aquaculture and Marine biotechnology Taskforce

(2011-2014)

PUblICATIONs (2011)

bhaskar, N.

1. Rai, A.K., Jini, R., Swapna, H.C., Baskaran,V., Sachindra, N.M. and bhaskar, N. (2011). Application of native lactic acid bacteria for fermentative recovery of lipids and proteins from fish processing waste: Bioactivities of fermentation products. Journal of Aquatic Food Product Technology 20, 32 – 44.

2. Jinni, R., Swapna, H.C, Rai, A.K., Vrinda, R., Halami, P.M., Sachindra, N.M. and bhaskar, N. (2011). Isolation and characterization of potential lactic acid bacteria (LAB) from freshwater fish processing wastes for application in fermentative utilization of fish processing waste. Brazilian Journal of Microbiology 42, 1516–1525.

Jeyasekaran, G.

3. Jeyasekaran, G., Thirumalai Raj, K., Jeya Shakila, R., Jemila Thangarani, A. and Sukumar, D. (2011a). Multiplex polymerase chain reaction-based assay for the specific detection of toxin producing Vibrio cholerae in fish and fishery products. Applied Microbiology and Biotechnology 90:1111-1118.

4. Jeyasekaran, G., Thirumalai Raj, K., Jeya Shakila, R., Jemila Thangaranai, A. and Sukumar, D. (2011b). Detection of Salmonella enterica serovars in shrimps in eight hours by Multiplex PCR assay. Annals of Microbiology. DOI: 10.1007/s13213-011-0250-5.

5. Thirumalai Raj, K., Jeyasekaran, G., Jeya Shakila, R., Jemila Thangarani, A. and Sukumar, D. (2011). Multiplex polymerase chain reaction assay for the detection of Salmonella enterica serovars in shrimps in 4 h. Journal of Bacteriology Research 3:56-62

Jeya shakila, R.

6. Muralidharan, N., Jeya shakila, R., Sukumar, D. and Jeyasekaran, G. (2011). “Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization”. Journal of Food Science and Technology. DOI: 10.1007/s13197-011-0440-y.

Jithendran, K.P.

7. Jithendran, K.P., Shekhar, M.S., Kannappan, S. and Azad, I.S. (2011). Nodavirus infection in freshwater ornamental fishes in India-diagnostic histopathology and nested RT-PCR. Asian Fisheries Science, 24: 12-19.

Karunasagar, I.

8. Neema, M., Karunasagar, I. and Karunasagar, I. (2011). Antigenic epitopes and MHC binders in Omp A of fish pathogen Edwardsiella tarda: A bioinformatics study. Biotechnology, Bioinformatics and Bioengineering 1, 39 – 45.

9. Neema, M., Karunasagar, I. and Karunasagar, I. (2011). Structural and functional characterization of outer membrane protein N in Edwardsiella ictaluri: A bioinformatic approach. International Journal of Pharmaceutical Sciences Review and Research 2, 13 – 26.


10. Maiti, B., Shetty, M., Shekar, M., Karunasagar, I. and Karunasagar, I. (2011). Recombinant outer membrane protein A (OmpA) of Edwardsiella tarda, a potential vaccine candidate for fish, common carp. Microbiological Research 167, 1–7.

11. Kumar, B.K., Raghunath, P., Devegowda, D., Deekshit, V.K., Venugopal, M.N., Karunasagar, I. and Karunasagar, I. (2011). Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood. International Journal of Food Microbiology 145, 244–249.

12. Rai, P., Safeena, M.P., Karunasagar, I. and Karunasagar. I. (2011). Complete nucleic acid sequence of Penaeus stylirostris densovirus (PstDNV) from India. Virus research 158, 37–45 (Impact Factor: 2.827).

13. Adiga, R., Karunasagar, I. and Karunasagar, I. (2011). Outer membrane protein secretin of type III secretion system of Vibrio vulnificus: structure prediction and orientation Bioinformatics 3, 61–66.

14. Adiga, R., Karunasagar, I. and Karunasagar, I. (2011). Molecular Docking Studies of Type III Secretion System Effector Sopb Homolog in Vibrio vulnificus. Journal of Computer Science and Systems Biology 4, 16–20.

15. Bhowmick, P.P., Devegowda, D., Ruwandeepika, H.A., Karunasagar, I. and Karunasagar, I. (2011). Presence of Salmonella pathogenicity island 2 genes in seafood-associated Salmonella serovars and the role of the sseC gene in survival of Salmonella enterica serovar Weltevreden in epithelial cells. Microbiology 157, 160–168.

Khare, s.K.

16. Ram, K., Kumar, R., Singh, M., Kapoor, S. and Khare, s.K. (2011). Gene identification and molecular characterization of solvent stable protease from a moderately haloalkaliphilic bacterium Geomicrobium sp. EMB2. Journal of Microbiology and Biotechnology 21, 129-135.

17. Ram, K. and Khare, s. K. (2011). Stability of haloalkaliphilic Geomicrobium sp. protease modulated by salt. biochemistry (Moscow) 76(6):686-693.

Mathivanan, N.

18. Krishnaraj, M. and Mathivanan, N. (2011). Diversity of marine actinomycetes in the Bay of Bengal and their antibacterial activity against human pathogens. Journal of Marine Biological Association of India 53: 135-138.

Mukherjee, J.

19. Sarkar, S., Roy, D. and Mukherjee, J. (2011). Enhanced protease production in a polymethylmethacrylate conico-cylindrical flask by two biofilm-forming bacteria. Bioresource Technology 102, 1849 – 1855.

20. Mitra, S., Sarkar, S., Gachhui, R. and Mukherjee, J. (2011a). A novel conico-cylindrical flask aids easy identification of critical process parameters for cultivation of marine bacteria. Applied Microbiology and Biotechnology 90, 321–330.

21. Mitra, S., Banerjee, P., Gachhui, R. and Mukherjee, J. (2011b). Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask. Bioprocess and Biosystems Engineering 34, 1087–1101.


Muraleedhara K. G.

22. Mohsin, S. and Muraleedhara, K.G. (2011). Mechanism underlying the anti-inflammatory effect of sulphated polysaccharide from Padina tetrastromatica against carrageenan induced paw edema in rats. Biomedicine and Preventive Nutrition 1, 294-301.

23. Mohsin, S., Arun, A.R. and Muraleedhara K. G. (2011). Anti inflammatory and antioxidant effect of sulphated polysaccharide isolated from marine algae Padina tetrastromatica from Kerala coast. Journal of Pharmacy Research 4, 784-787.

Nagaraju, T.

24. Mamata, M., Sridhar, G., Ravi Kumar Reddy, K., Nagaraju, T. and Padma, T. (2011). Is the variant c.422+90G A in intron 4 of Indoleamine 2, 3 -dioxygenase (IDO) gene related to age related cataracts? Molecular Vision, 17, 1203-1208.

Pandian, s. K.

25. Nithyanand, P., Manju, S. and Pandian, S. K. (2011). Phylogenetic characterization of culturable actinomycetes associated with the mucus of the coral Acropora digitifera from Gulf of Mannar. FEMS Microbiology Letters 314,112-118

26. Nithyanand, P., Indhumathi, T., Ravi, A.V. and Pandian, s.K. (2011). Culture independent characterization of bacteria associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar. World Journal of Microbiology and Biotechnology 27, 1399 – 1406.

Roy, R.

27. Manju, M. and Roy, R. (2011). Fish lipid prevents alcohol induced damages in liver and kidney tissues of mice (Mus musculus). International Journal of Integrative Biology 11, 14 – 20 .

sachindra, N.M.

28. Meenata, K., Sowmya, R., Rathina, R. K. and sachindra, N. M. (2011). Antioxidant activity of protein isolate obtained by pH shift technique from hydrolysed and unhydrolysed shrimp processing discards. Journal of Aquatic Food Product Technology 20, 209–221.

29. Sowmya, R., Rathinaraj, K. and sachindra, N.M. (2011). An autolytic process for recovery of antioxidant activity rich carotenoprotein from shrimp heads. Marine Biotechnology 13, 918–927 (Impact Factor: 3.152).

sahul Hameed, A.s.

30. Babu, V.S., Nambi, K.S., Chandra, V., Ishaq Ahmed, V.P., Bhonde, R. and sahul Hameed, A.s. (2011). Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.). Journal of Fish Diseases 34, 355–364.

sambasiva Rao., K.R.s.

31. Suresh Kumar, P., Ghosh, M., Pulicherla, K.K. and sambasiva Rao, K.R.s. (2011). Cold Active Enzymes from the Marine Psychrophiles: Biotechnological Perspective. Advanced Biotechnology, 10, 16-20.

32. Suresh Kumar, P., Pulicherla, K.K., Ghosh, M., Kumar, A. and sambasiva Rao, K.R.s. (2011). Structural prediction and comparative docking studies of psychrophilic β- Galactosidase with lactose, ONPG and PNPG against to its counter parts of mesophilic and thermophilic enzymes. Bioinformation, 6, 311-314.


shekher, M.s.

33. shekhar, M.s., Sahoo P.K., Dillikumar, M. and Abhilipsa Das (2011). Cloning, expression and sequence analysis of Macrobrachium rosenbergii nodavirus genes: Indian isolate. Aquaculture Research, 42, 1778-1788.

suryanarayanan, T.s.

34. Thirunavukkarasu, N., suryanarayanan, T. s., Girivasan, K. P., Venkatachalam, A., Geetha, V., Ravishankar, J.P. and Doble, M. (2011a). Fungal symbionts of marine sponges from Rameswaram, southern India: species composition and bioactive metabolites. Fungal Divers. DOI 10.1007/s13225-011-0137-6.

35. Thirunavukkarasu, N., suryanarayanan, T.s., Murali, T.S., Ravishankar, J.P. and Gummadi, S.N. (2011b). L-asparaginase from marine derived fungal endophytes of seaweeds. Mycosphere 2, 147–155.

Vaseeharan, b.

36. Vaseeharan, b. and Valli, S. (2011). In silico Homology Modeling of Prophenoloxidase activating factor Serine Proteinase Gene from the Haemocytes of Fenneropenaeus indicus. Journal of Proteomics and Bioinformatics 4, 53–57 .

37. Vaseeharan, b., Shanthi, S. and Prabhu, N.M. (2011). A novel clip domain serine proteinase (SPs) gene from the haemocytes of Indian white shrimp Fenneropenaeus indicus: molecular cloning, characterization and expression analysis. Fish and Shellfish Immunology 30, 980–985.


PUblICATIONs (2012)

Aparna, D.

1. Chhabra, G., Upadhyaya, T. and Dixit, A. (2012). Molecular cloning, sequence analysis and structure modeling of OmpR, the response regulator of Aeromonas hydrophila. Molecular Biology Reports, 39, 41 - 50.

balaram, P.

2. Gupta, K., Kumar, M., Chandrashekara, K., Krishnan, and K.S., balaram, P. (2012). Combined electron transfer dissociation-collision-induced dissociation fragmentation in the mass spectrometric distinction of leucine, isoleucine, and hydroxyproline residues in Peptide natural products. Journal of Proteome Research 11, 515 – 522.

3. Khoo, K.K., Gupta, K., Green, B.R., Zhang, M.M., Watkins, M., Olivera, B.M., balaram, P., Yoshikami, D., Bulaj, G. and Norton, R.S. (2012). Distinct disulfide isomers of μ-conotoxins KIIIA and KIIIB block voltage-gated sodium channels. Biochemistry, 51, 9826 – 9835.

barat, A.

4. Chandra, S., barat, A., Singh, M., Singh, B. K. and Matura, R. (2012). DNA Bar-Coding of Indian Coldwater Fishes of Genus Schizothorax (Family: Cyprinidae) from Western Himalaya. World Journal of Fish and Marine Sciences, 4, 430 – 435.

5. A’Hara, S.W., Amouroux, P., Argo, E.E., Avand-Faghih, A,, and barat, A., et al. (2012). Permanent genetic resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011. Molecular Ecology Resources, 12, 185-189.

basavaraja, N.

6. basavaraja, N., Chandrashekhara, B.H. and Rather, M.A. (2012). Production of an all-male population of guppy, Poecilia reticulata (Schneider). Current Science, 103, 1151-1152.

bhaskar, N.

7. Rai, A.K., Swapna, H.C., bhaskar, N. and Baskaran V. (2012). Potential of seafood industry byproducts as sources of recoverable lipids: Fatty acid composition of meat and non meat component of selected Indian marine fishes Journal of Food Biochemistry, 36, 441-448.

8. Hemavathi, D., Swapna, H.C. and bhaskar, N. (2012). Lipid class and fatty acid composition of meat and non-meat components of selected sea foods. Indian Journal of Fisheries, 59, 133-139.

9. Vrinda, R., Bijinu, B., Rai, A.K., Bhaskar, N. and Prakash, M.H. (2012). Concomitant production of lipase, protease and enterocin by Enterococcus faecium NCIM5363 and Enterococcus durans NCIM5427 isolated from fish processing waste. International Aquatic Research, 4, 14.

biju Kumar, A.

10. Sreeja, V., biju Kumar, A. and Norman, M. (2012). First report of Amphioctopus neglectus (Nateewathana & Norman, 1999) and A. rex (Nateewathana & Norman, 1999) (Mollusca: Cephalopoda) from the Indian coast. Molluscan Research, 32(1): 43–49.


Chakrabarti, R.

11. Srivastava, P. K. and Chakrabarti, R. (2012). Effect of dietary supplementation with Achyranthes aspera seed on larval rohu Labeo rohita challenged with Aeromonas hydrophila. Journal of Aquatic Animal Health, 24, 213-218.

12. Srivastava, P.K. and Chakrabarti, R. (2012). Effect of Dietary Supplementation of Achyranthes aspera Seed on the Immune System of Labeo rohita Fry. The Israeli Journal of Aquaculture, 64,786-792.

13. Chakrabarti, R. and Rao, V.Y. (2012). Achyranthes aspera enhances immunity and antigen clearance in common carp, Cyprinus carpio L. Journal of Fish Diseases, 35, 389-392.

14. Chakrabarti, R., Srivastava, P. K., Kundu, K., Khare, R.S. and Banerjee, S. (2012). Evaluation of immunostimulatory and growth promoting effect of seed fractions of Achyranthes aspera in common carp Cyprinus carpio and identification of active constituents. Fish and Shellfish Immunology, 32,839-843.

Das, P.

15. Sahu, B.P., Patel, A., Sahoo, L., Das, P., Meher, P.K. and Jayasankar, P. (2012). Rapid and cost effective development of SSR markers using next generation sequencing in Indian major carp, Labeo rohita (Hamilton, 1822). Indian Journal of Fisheries, 59, 21-24.

De, D.

16. De, D., Ghoshal, T.K. and Ananda Raja, R. (2012). Characterization of enzyme-producing bacteria isolated from the gut of Asian seabass, Lates calcarifer and milkfish, Chanos chanos and their application for nutrient enrichment of feed ingredients. Aquaculture Research, doi: 10.1111/are.12099.

Goswami M

17. Goswami, M., Sharma, B.S., Tripathi, A.K., Yadav, K., Bahuguna, S.N., Nagpure, N.S., Lakra, W.S. and Jena, J.K. (2012). Development and characterization of cell culture systems from Puntius (Tor) chelynoides (McClelland). Gene, 500, 140-147.

Idris, M.M.

18. Saxena, S., Singh, S.K., Meena Lakshmi, M.G., Meghah, V., Bhatti, B., Brahmendra Swamy, C.V., Sundaram, C.S. and Idris, M.M. (2012). Proteomics analysis of zebrafish caudal fin regeneration. Molecular & Cellular Proteomics, 11(6): M111.014118. doi: 10.1074/mcp.M111.014118.

Immanuel, G.

19. Sivagnanavelmurugan, M., Maruthupandi, T., Palavesam, A. and Immanuel, G. (2012). Antiviral effect of fucoidan extracted from the brown seaweed, Sargassum wightii, on shrimp Penaeus monodon postlarvae against white spot syndrome virus. Journal of the World Aquaculture Society, 43, 697 – 706.

20. Immanuel G., Sivagnanavelmurugan M., Balasubramanian, V. and Palavesam, A. (2012). Sodium alginate from Sargassum wightii retards mortalities in Penaeus monodon postlarvae challenged with white spot syndrome virus. Diseases of Aquatic organisms, 99, 187-196.

Jeya shakila, R.

21. Jeya shakila, R., Jeevithan, E., Varatharajakumar, A., Jeyasekaran, G. and Sukumar, D. (2012). Functional characterization of gelatin extracted from bones of red snapperand grouper in comparison with mammalian gelatin. LWT - Food Science and Technology, 48: 30-36.


Karunasagar, I.

22. Khushiramani, R.M., Maiti, B., Shekar, M., Girisha, S.K., Akash, N., Deepanjali,A., Karunasagar, I. and Karunasagar, I. (2012). Recombinant Aeromonas hydrophila outer membrane protein 48 (Omp48) induces a protective immune response against Aeromonas hydrophila and Edwardsiella tarda. Research in Microbiology, 163, 286-291.

23. Maiti, B., Shetty, M., Shekar, M., Karunasagar, I. and Karunasagar, I. (2012). Evaluation of two outer membrane proteins, Aha1 and OmpW of Aeromonas hydrophila as vaccine candidate for common carp. Veterinary Immunology and Immunopathology, 149, 298-301.

24. Hamod, M.A., Nithin, M.S., Shukur, Y.N., Karunasagar, I. and Karunasagar I. (2012). Outer membrane protein K as a subunit vaccine against V. anguillarum. Aquaculture, 354-355, 107-110.

25. Pradeep, B, Rai, P., Mohan, S.A., Shekhar, M.S. and Karunasagar, I. (2012). Biology, host range, pathogenesis and diagnosis of white spot syndrome virus. Indian Journal of Virology, 23, 161-174.

26. Rai, P., Safeena, M.P., Krabsetsve, K., La Fauce, K., Owens, L. and Karunasagar, I. (2012). Genomics, molecular epidemiology and diagnostics of infectious hypodermal and hematopoietic necrosis virus. Indian Journal of Virology, 23: 203-214.

27. Safeena, M.P., Rai, P. and Karunasagar, I. (2012). Molecular biology and epidemiology of hepatopancreatic parvovirus of penaeid shrimp. Indian Journal of Virology, 23, 191-202.

Maitra, s.K.

28. Moniruzzaman, M. and Maitra, s. K. (2012). Influence of altered photoperiods on serum melatonin and its receptors (MT1 and MT2) in the brain, retina, and ovary in carp Catla catla. Chronobiology International, 29, 175–188.

29. Pal, P., Hasan, K. N. and Maitra, s. K. (2012). Functional pleiotropy of melatonin in the regulation of reproduction: An overview. Journal of Endocrinology & Reproduction, 16, 33-43.

Mazumder, s.

30. Banerjee, C., Goswami, R., Verma, G., Datta, M. and Mazumder, s. (2012). Aeromonas hydrophilainduced head kidney macrophage apoptosis in Clarias batrachus involves the activation of calpain and is caspase-3 mediated. Developmental and Comparative Immunology, 37, 323-333.

Mukherjee, J.

31. Mitra, S., Thawrani, D., Banerjee, P., Gachhui, R. and Mukherjee, J. (2012). Induced biofilm cultivation enhances riboflavin production by an intertidally derived Candida famata. Applied Biochemistry and Biotechnology, 166, 1991-2006.

Riji John, K.

32. Riji John, K. and Rosalind George, M. (2012). Viruses associated with epizootic ulcerative syndrome (EUS) – an update. Indian Journal of Virology, 23, 106–113.

Roy, R.

33. Pujari, P. and Roy, R. (2012). Dietary polyunsaturated fatty acids alleviate D-galcatosamine induced hepatitis by regulating cytokine production. International Journal of Integrative Biology 13, 24-29.


sachindra N.M.,

34. Sowmya, R., and sachindra, N.M. (2012). Evaluation of antioxidant activity of carotenoid extract from shrimp processing byproducts by in-vitro assays and in membrane model system. Food Chemistry, 134, 308-314.

35. Sowmya, R., Ravikumar, T.M., Vivek, R., Rathinaraj, K. and sachindra, N.M. (2012). Optimization of enzymatic hydrolysis of shrimp waste for recovery of antioxidant activity rich protein isolate. Journal of Food Science and Technology, DOI 10.1007/s13197-012-0815-8.

sahoo, P.K.

36. Robinson, N., sahoo, P.K., Baranski, M., Das Mahapatra, K., Saha, J.N., Das, S., Mishra, Y., Das, P., Barman, H.K. and Eknath, A.E. (2012). Expressed sequences and polymorphisms in rohu carp (Labeo rohita, Hamilton) revealed by mRNA-seq. Marine Biotechnology, 14, 620-633 (Impact Factor: 3.152).

37. sahoo, P.K., Shekhar, M.S., Abhilipsa Das., Dilli Kumar, M., Bindu R Pillai. and Sahul Hameed, A.S. (2012). Immunomodulatory effect of recombinant RNA-dependent RNA polymerase protein of Macrobrachium rosenbergii nodavirus in giant freshwater prawn M. rosenbergii. Aquaculture Research, 43 (8), 1096–1106 .

sahul Hameed, A.s.

38. Babu, V.S., Chandra, V., Nambi, K.S.N., Majeed, S.A., Taju, G., Patole, M.S. and sahul Hameed, A.s. (2012). Development and characterization of novel cell lines from Etroplus suratensis and their applications in virology, toxicology and gene expression. Journal of Fish Biology, 80, 312-334.

39. Taju, G., Abdul Majeed, S., Nambi, K.S. N., Sarath Babu, V., Vimal, S., Kamatchiammal, S., and sahul Hameed, A.s. (2012). Comparison of in vitro and in vivo acute toxicity assays in Etroplus suratensis (Bloch, 1790) and its three cell lines in relation to tannery effluent. Chemosphere, 87 55-61.

40. Sundar Raj, N., Nambi, K.S.N., Abdul Majeed, S., Taju, G., Vimal, S., Farook, M.A., and sahul Hameed, A.s. (2012). High efficacy of WSSV replication in tissues of freshwater rice-field crab, Paratelphusa hydrodomus (Herbst). Journal of Fish Disease, 35, 917- 925.

41. Nathiga Nambi, K.S., Abdul Majeed, S., Sundar Raj, N., Taju, G., Madan, N., Vimal, S. and sahul Hameed, A.s. (2012). In vitro white spot syndrome virus (WSSV) replication in explants of heart of freshwater crab, Paratelphusa hydrodomous. Journal of Virological Methods, 183, 186-195.

sambasiva Rao, K.R.s.

42. Ghosh, M., Pulicherla, K. K. Rekha, V.P.B. and sambasiva Rao, K.R.s. (2012). Cold active β-galactosidase from Thalassospira sp. 3SC-21 to use in milk lactose hydrolysis: A novel source from deep waters of Bay-of-Bengal. World Journal of Microbiology and Biotechnology, 28, 2859 – 2869.

santhanam, P.

43. Jeyaraj, N. and santhanam, P. (2012). Influence of algal diet on population density, egg production and hatching succession of the calanoid copepod, Paracalanus parvus (Claus, 1863). Journal of Algal Biomass Utilization, 4, 1-8.

sarita G. bhat

44. Sapna, K., Manzur Ali, P., Abraham Mathew, Rekha mol, K.R., sarita G. bhat, Chandrasekaran, M. and Elyas, K.K. (2012). Marine Pseudomonas mendocina BTMW 301 as a potential source for extracellular proteinaceous protease inhibitor. Advanced Biotech, 11, 16-19.


senthilkumaran, b.

45. Chakrabarty, S., Rajakumar, A., Raghuveer, K., Sridevi, P., Mohanachary, A., Prathibha, Y., Bashyam, L., Dutta-Gupta, A. and senthilkumaran, b. (2012). Endosulfan and flutamide, alone and in combination, target ovarian growth in the juvenile catfish, Clarias batrachus. Comparative biochemistry and physiology. Toxicology & pharmacology, 155, 491-497.

46. Rajakumar, A., Singh, R., Chakrabarty, S., Murugananthkumar, R., Laldinsangi, C., Prathibha, Y., Sudhakumari, C.C., Dutta-Gupta, A. and senthilkumaran, b. (2012). Endosulfan and flutamide impair testicular development in the juvenile Asian catfish, Clarias batrachus, Aquatic Toxicology, 110-111, 123-132.

Vaseeharan, b.

47. Vaseeharan, b., Shanthi, S., Chen, J.C. and Montserrat, E. (2012). Molecular cloning, sequence analysis and expression of Fein-Penaeidin from the haemocytes of Indian white shrimp Fenneropenaeus indicus. Results in Immunology, 2, 35–43.

48. Shanthi, S. and Vaseeharan, b. (2012). cDNA cloning, characterization and expression analysis of a novel antimicrobial peptide gene penaeidin-3 (Fi-Pen3) from the haemocytes of Indian white shrimp Fenneropenaeus indicus. Microbiological Research, 167, 127-134.

49. Pallela, R., Venkateswara Rao, J., Janapala, V.R. and Kim, S-K. (2012). Biophysicochemical evaluation of chitosan-hydroxyapatite-marine sponge collagen composite for bone tissue engineering. Journal of Biomedical Materials Research Part A, 100A (2), 486–495.


PUblICATIONs (2013)

Alavandi, s. V.

1. Satheesh Kumar, S., Ananda Bharathi, R., Rajan, J.J.S., Alavandi , s.V., Poornima, M., Balasubramanian C.P. and Ponniah, A.G. (2013). Viability of White spot syndrome virus (WSSV) in sediment during sun-drying (drainable pond) and under non-drainable pond conditions indicated by infectivity to shrimp. Aquaculture, 402-403,119–126.

Arumugam, M.

2. Radha, M., Mullainadhan, P. and Arumugam, M. (2013). Detection of two distinct types of hemolymphatic prophenoloxidase and their differential responses in black tiger shrimp, Penaeus monodon, upon infection by white spot syndrome virus. Aquaculture, 376-379, 76 -84.

Arumugam, M.

3. Giji, S., Arumugam, M., Abirami, P. and Balasubramanian, T. (2013). Isolation and characterization of hyaluronic acid from the liver of marine stingray Aetobatus narinari. International Journal of Biological Macromolecules, 54, 84 – 89.

4. Kanchana, S., Arumugam, M., Giji, S. and Balasubramanian, T., 2013. Isolation, characterization and antioxidant activity of hyaluronic acid from marine bivalve mollusc Amussium pleuronectus (Linnaeus, 1758). Bioactive Carbohydrates and Dietary Fibre, 2, 1-7.

balaram, P.

5. Bhattacharyy, M., Gupta, K., Gowd, K.H. and balaram, P. (2013). Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins. Molecular BioSystems, 9, 1340 – 1350.

6. Sonti, R., Gowd, K. H, Rao, K.N., Ragothama, S., Rodriguez, A., Perez, J. J. and balaram, P. (2013). Conformational diversity in contryphans from Conus venom: cis-trans isomerisation and aromatic/proline interactions in the 23-membered ring of a 7-residue peptide disulfide loop. Chemistry - A European Journal, 19, 15175 – 15189.

7. Kuang, Z., Zhang, M.M., Gupta, K., Gajewiak, J., Gulyas, J., balaram, P., Rivier, J.E. Olivera, B.M., Yoshikami, D., Bulaj, G. and Norton, R.S. (2013). Mammalian neuronal sodium channel blocker μ-conotoxinBuIIIB has a structured N terminus that influences potency. ACS Chemical Biology 8, 1344 -1351.

biju Kumar, A.

8. Sreeja, V. and bijukumar, A. (2013). Ethological studies of the Veined Octopus Amphioctopus marginatus (Taki) (Cephalopoda: Octopodidae) in captivity, Kerala, India. Journal of Threatened Taxa 5, 4492–4497.

9. Sneha Chandran, B.K., Ravinesh, R. and biju Kumar, A. (2013). Record of the dendrodorid slug, Dendrodoris coronata (Nudibranchia: Dendrodorididae) from Western Indian Ocean. Marine Biodiversity Records, Marine Biological Association of the United Kingdom, 6, e116.

10. Sreeja, V., Anushma, K. and biju Kumar, A. (2013). Embryonic development of Amphioctopus marginatus (Taki) (Mollusca: Octopodidae) in captivity. Journal of Aquatic Biology & Fisheries, 1 (1&2) 165-173.


Chakrabarti, R.

11. Singh, K.M., Sharma, J.G. and Chakrabarti, R., (2013a). Impact of UV-B Radiation on the physiology of freshwater carp Labeo rohita larvae and evaluation of UV-B protective properties of seeds of Achyranthes aspera and vitamin C. Agricultural Research, 2, 166-171.

12. Singh, K. M., Sharma, J. G. and Chakrabarti, R. (2013b). Effect of UV-B radiation on the defense system of Labeo rohita (Cyprinidae) larvae and its modulation by seed of Achyranthes aspera. Acta Ichthyologica et Piscatoria (Scientific Society of Szczecin), 43, 119-126.

Das, s.K.

13. Badhai, J., Kumari, P., Krishnan, P., Ramamurthy, T. and Das, s.K. (2013). Presence of SXT integrating conjugative element in marine bacteria isolated from the mucus of the coral Fungia echinata from Andaman Sea. FEMS Microbiology Letters, 338, 118-123.

De, D.

14. De, D., Ghoshal, T.K., Ananda Raja, R. and Kumar, S. (2013). Growth performance, nutrient digestibility and digestive enzyme activity in Asian seabass, Lates calcarifer juveniles fed diets supplemented with cellulolytic and amylolytic gut bacteria isolated from brackishwater fish. Aquaculture Research, doi:10.1111/are.12325.

Goswami, M.

15. Goswami, M., Sharma, B.S., Bahuguna, S.N., Nagpure, N.S. and Lakra W.S. (2013). A SRCF cell line from snow trout, Schizothorax richardsonii: development and characterization. Tissue and Cell, 45, 219-226.

16. Goswami M, Yadav, K., Dubey, A., Sharma, B.S., Konwar, R., Kumar, R., Nagpure, N.S. and Lakra, W.S. (2013). In vitro cytotoxicity assessment of two heavy metal salts in a fish cell line (RF). Drug and Chemical Toxicology. 37(1):48-54.

Idris, M.M.

17. Saxena, S., Dupont, S., Meghah, V., Meena Lakshmi, M.G., Singh, S.K., Swamy, C.V.B. and Idris,M.M. (2013). Proteome map of the neural complex of the Tunicate Ciona intestinalis, the closest living relative to vertebrates. Proteomics, 13(5): 860-65.

Jithendran, K.P.

18. Binesh, C.P. and Jithendran, K.P. (2013). Genetic characterization of betanodavirus isolates from Asian seabass Lates calcarifer (Bloch) in India. Archives of Virology, 158: 1543-1546.

19. Binesh, C.P., Greeshma, C. and Jithendran, K.P. (2013). Genomic classification of betanodavirus by molecular phylogenetic analysis of the coat protein gene. Archives of Virology, 158: 1589-1595.

Joydeep, M.

20. Mitra, S., Arnab, P., Srijoni, B., Saubhik, H., Ratan, G. and Joydeep, M. (2013). Enhanced biotransformation of fluoranthene by intertidally derived Cunninghamella elegans under biofilm-based and niche-mimicking conditions. Applied and Environmental Microbiology, 79, 7922-7930.

Jyothi, s.

21. Sucharita, V. and Jyothi, s. (2013). An identification of penaeid prawn species based on histogram values. International Journal of Advanced Research in Computer Application and Software Engineering, 3, 807-811.


22. Sucharita, V., Jyothi, s. and Mamtha, D.M. (2013). A Comparative study on various edge detection techniques used for the identification of penaeid prawn species. International Journal of Computer Application, 78, 1-5.

Karunasagar, I.

23. Naveen Kumar, S., Shekar, M., Karunasagar, I. and Karunasagar, I. (2013a). Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India. Virus Research, 173, 377-385.

24. Naveen Kumar, S., Karunasagar, I. and Karunasagar, I. (2013b). Protection of Macrobrachium rosenbergii against white tail disease by oral administration of bacterial expressed and encapsulated double-stranded RNA. Fish and Shellfish Immunology, 35, 833-839.

Kannappan, s.

25. Kannappan, S, Sivakumar, K. and Patil, P.K. (2013a). Effect of garlic extract on the luciferase, bio-luminescence, virulence factors produced by Vibrio harveyi with a challenge during Penaeus monodon larviculture. African Journal of Microbiology Research, 7, 1766 -1779.

26. Kannappan, s., Sivakumar, K., Patil, P.K. and Richard Kennedy, R.N. (2013b). Extraction and detection of N-acyl homoserine lactones from shrimp pathogen Vibrio harveyi and antagonistic effect of terrestrial plants against its growth. African Journal of Microbiology Research, 7, 3275- 3284.

27. Sivakumar, K. and Kannappan, s. (2013). Inhibitory effect of marine algae collected from the East and West coast of India against luciferase and luminescence producing Vibrio harveyi. African Journal of Biotechnology, 12, 3493- 3502.

Maitra, s.K.

28. Maitra, s. K., Chattoraj, A., Mukherjee, S. and Moniruzzaman M. (2013). Melatonin: A potent candidate in the regulation of fish oocyte growth and maturation. General and Comparative Endocrinology, 181, 215–222.

Mukherjee, J.

29. Mitra, S., Pramanik, A., Banerjee, S., Haldar, S., Gachhui, R. and Mukherjee, J. (2013). Enhanced biotransformation of fluoranthene by intertidally derived Cunninghamella elegans under biofilm-based and niche-mimicking conditions. Applied and Environmental Microbiology 79, 7922-7930.

Muraleedhara, K. G.

30. Mohsin, S., Kurup, G.M. and Mahadevan, R. (2013). Effect of ascophyllan from brown algae Padina tetrastromatica on inflammation and oxidative stress in carrageenan-induced rats. Inflammation, 36, 1268-1278.

Nandi, s.

31. Sahu, D.K., Panda, S.P. Panda, S., Das, P., Meher, P.K., Hazra, R.K., Peatman, E., Liu, Z.J., Eknath, A.E. and Nandi, S. (2013). Identification of reproduction-related genes and SSR-markers through expressed sequence tags analysis of a monsoon breeding carp rohu, Labeo rohita (Hamilton) Gene, 524, 1-14.


Rosamma, P.

32. Deepthi, A., Jimly, J.C., Ramya, K.D. and Rosamma Philip. (2013). Actinobacteria from sediment samples of Arabian sea and Bay of Bengal: Biochemical and physiological characterization. International Journal of Research in Marine Sciences, 2, 56-63.

sahoo, P.K.

33. sahoo, P.K., Das, S.K, Mahapatra, K.D., Saha, J.N., Baranski, M., Ødegård, J. and Robinson, N. (2013). Characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to Aeromonas hydrophila resistance in rohu, Labeo rohita. Fish and Shellfish Immunology, 34, 1325-1334.

sahul Hameed, A.s.

34. Abdul Majeed, S., Nambi, K.S.N., Taju, G. and sahul Hameed, A.s. (2013a). Development, characterization and application of a new fibroblastic-like cell line from kidney of a freshwater air breathing fish Channa striatus (Bloch, 1793). Acta Tropica, 127, 25-32.

35. Abdul Majeed, S., Nambi, K. S. N., Taju, G., Sundar Raj, N., Madan, N. and sahul Hameed, A. s. (2013b). Establishment and characterization of permanent cell line from gill tissue of Labeo rohita (Hamilton) and its application in gene expression and toxicology. Cell Biology and Toxicology, 29, 59-73.

36. Farook, M.A., Madan, N., Taju, G., Abdul Majeed, S., Nambi, K.S.N., Sundar Raj, N., Vimal, S. and sahul Hameed, A.s. (2013). Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r-MCP43) of giant freshwater prawn, Macrobrachium rosenbergii (de Man) for immunological diagnostic methods. Journal of Fish Diseases, 37, 703-710.

37. Madan, N., Nambi, K.S.N., Abdul Majeed, S., Taju, G., Sundar Raj, N., Farook, M.A., Vimal, S. and sahul Hameed. A.s. (2013a). In Vitro Propagation of Hepatopancreatic Parvo-like Virus (HPV) of Shrimp in C6/36 (Aedes albopictus) cell line. Journal of Invertebrate Pathology, 112, 229–235.

38. Madan, N., Sundar Raj, N., Farook, M.A., Vimal, S., Venkatesan, C., Abdul Majeed, S., Nambi, K.S.N. and sahul Hameed, A.s. (2013b). Partial cloning and production of polyclonal antiserum against recombinant capsid protein of Hepatopancreatic Parvovirus (HPV) and its application for diagnostics in penaeid shrimp. Process Biochemistry, 48, 1893-1898.

39. Sarath Babu, V., Abdul Majeed, S., Nambi, K.S.N., Taju, G., Madan, N., Sundar Raj, N., sahul Hameed, A.s. (2013). Comparison on betanodavirus replication efficiency in ten Indian fish cell lines. Archives of Virology, 158, 1367-1375.

40. Taju, G., Abdul Majeed, S., Nambi, K.S.N. and sahul Hameed, A.s. (2013). Development and characterization of cell line from the gill tissue of Catla catla (Hamilton, 1822) for toxicological studies. Chemosphere, 90, 2172–2180.

santhanam, P.,

41. santhanam, P., Jeyaraj, N. and Jothiraj, K. (2013). Effect of temperature and algal food on egg production and hatching of copepod, Paracalanus parvus (Claus). Journal of Environmental Biology, 34, 243-246 (No Impact Factor).


sarkar, U.K.,

42. Raizada, S., Lal, K.K., sarkar, U.K., Varshney, P.K., Sahu, V., Yadav, K.C., Agnihotri, P., Awasthi, A. and Jena J.K. (2013). Captive breeding and embryonic development of Butter Catfish (Ompok bimaculatus, Bloch 1794), a threatened fish of Indian subcontinent in Northern India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 83, 333-339 (Impact Factor: 0.396).

43. Mishra, S.K., sarkar, U.K., Trivedi, S.P., Mir J.I. and Pal, A. (2013). Biological parameters of a silurid catfish Ompok bimaculatus (Bloch, 1794) from River Ghaghara, India. Journal of Environmental Biology, 34, 1013-1017 339.

Veera Ravi, A.

44. Packiavathy, I.A., Sasikumar, P., Pandian, S.K. and Veera Ravi, A. (2013). Prevention of quorum-sensing-mediated biofilm development and virulence factors production in Vibrio spp. by curcumin. Applied Microbiology and Biotechnology, 97, 10177-10187.

Venkateswara Rao, J.,

45. Koigoora, S., Ahmad, I., Pallela, R. and Venkateswara Rao, J. (2013). Spatial variation of potentially toxic elements in different grain size fractions of marine sediments from Gulf of Mannar, India. Environmental Monitoring and Assessment DOI 10.1007/s10661-013-3120-8.

46. Srikanth, K., Pallela, R., Nelakurti, C. R., Madhavendra, S.S. and Venkateswara Rao, J. (2013). Morphometric, elemental and biochemical characterization of three Haliclona species (Demospongiae) from the Gulf of Mannar, India. Chemical speciation & Bioavailability, 25, 15-23.

A haul of freshwater prawn


PUblICATIONs (2014)

Acharya, U.R.

1. Acharya, U.R., Behera, S.K., Swain, S.N., Panda, M.K., Mistri, A.R. and Sahoo, B.B. (2014). Sequence and structural analysis of β- actin protein of fishes, using bioinformatics tools and techniques. International Journal of Biosciences, 4, 249-256.

Aparna Dixit

2. Karan, S., Kaushik, H., Saini, N., Sahoo, P.K., Dixit, A. and Garg, L.C. (2014). Genomic cloning and sequence analysis of Interleukin-10 from Labeo rohita. Bioinformation, 10, 623–629.

3. Dash, P., Sahoo, P.K., Gupta, P.K., Garg, L.C. and Dixit, A. (2014). Immune responses and protective efficacy of recombinant outer membrane protein R (rOmpR)-based vaccine of Aeromonas hydrophila with a modified adjuvant formulation in rohu (Labeo rohita). Fish and Shellfish Immunology, 39, 512-523.

4. Yadav, S.K., Sahoo, P.K. and Dixit, A. (2014). Characterization of Immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model. Molecular Biology Reports, 41, 1837-1148.

balaram, P.

5. Sonti, R., Shashanka Rao, K. N., Chidanand, S., Gowd, K.H., Raghothama, S. and balaram, P. (2014) Conformational analysis of a 20-membered cyclic peptide disulfide from conusvirgo with a wpw segment: evidence for an aromatic–proline sandwich. Chemistry - A European Journal, 20, 5075 – 5086.

basavaraja, N.

6. basavaraja, N., Chandrashekhara, B.H. and Ahamad, R.M. (2014). Norethindrone-induced masculinization and progeny testing in guppy, Poecilia reticulata (Peters 1859). Indian Journal of Experimental Biology, 52, 232-236.

baskaran, V.

7. Ravi, H. and baskaran, V., (2014). Biodegradable chitosan-glycolipid hybrid nanogels: A novel approach to encapsulate fucoxanthin for improved stability and bioavailability. Food Hydrocolloids, 43, 717 – 725.

bhaskar, N.

8. Ruthu, Murthy, P.S., Rai, A.K. and bhaskar, N., 2014. Fermentative recovery of lipids and proteins from freshwater fish head waste with reference to antimicrobial and antioxidant properties of protein hydrolysate. Journal of Food Science and Technology, 59, 1884-1892.

9. Muhammed, M.A., Domendra, D., Muthukumar, S.P., Sakhare, P.Z. and bhaskar, N. (2014). Effect of fermentatively recovered fish waste lipids on the growth and composition of broiler meat. British Poultry Science 1-9.

bijoy Nandan, s.

10. Radhika, R., bijoy Nandan, s., Rithin Raj, M. and Sanu, V.F. (2014). Species assemblage and community patterns of cyclopoid copepods in Kavarathi atoll along the Indian Coast, International Journal of Current Research, 6, 8648-8657.


11. Sanu V.F., bijoy Nandan, s., Rithin Raj, M., Radhika, R. (2014). Mesozooplankton distribution in Kavaratti Atoll, Lakshadweep Archipelago, South West Coast of India with special reference to calanoid copepods, IOSR Journal of Environmental Science, Toxicology and Food Technology, 8, 69-78.

biju Kumar, A.

12. Preetha, K., Ravinesh, R., biju Kumar, A., Dhaneesh, K.V. and George, S. (2014). First record of Granulifusus poppei (Mollusca: Fasciolariidae) from Indian coast. Marine Biodiversity Records, 7, e95.

Chakrabarti, R.

13. Chakrabarti, R., Srivastava, P., Verma N. and Sharma, J. (2014). Effect of seeds of Achyranthes aspera on the immune responses and expression of some immune-related genes in carp Catla catla. Fish and Shellfish Immunology, 41, 64-69.

Chiplunkar, s.V.

14. Babita, B., Chiplunkar., s.V. and Madhavi, M.I. (2014). Methanol Extract of Euchelus asper prevents bone resorption in ovariectomised mice model. Journal of Osteoporosis, 348189.

15. Balakrishnan, B., Indap, M.M., Singh, S.P., Krishna, C. M. and Chiplunkar, s.V., (2014). Turbo methanol extract inhibits bone resorption through regulation of T cell function. Bone, 58, 114-125.

Das, P.

16. Sahoo, L., Sahu, B. P., Sofia P. Das, Subrat K. Swain, Bej, D., Patel, A., Jayasankar, P. and Das, P. (2014). Limited genetic differentiation in Labeo rohita (Hamilton 1822) populations as revealed by microsatellite markers. Biochemical Systematics and Ecology, 57, 427-431.

Das, s K.

17. Kamal, D., Poddar, A. and Das, s.K. (2014). Photobacterium panuliri sp. nov., an alkali tolerant marine bacterium isolated from eggs of spiny lobster, Panulirus penicillatus from Andaman Sea. Current Microbiology, 69, 660-668.

18. Kumari, P., Poddar, A., Schumann, P. and Das, s.K. (2014). Vibrio panuliri sp. nov., a marine bacterium isolated from spiny lobster, Panulirus penicillatus and transfer of Vibrio ponticus from Scophthalmi clade to the newly proposed Ponticus clade. Research in Microbiology, 165, 826-835.

19. Poddar, A., Lepcha, R.T., Mukherjee, D., Bhattacharyya, D. and Das s.K. (2014). Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei sp. nov., a novel marine bacterium isolated from the Andaman Sea. Research in Microbiology, 165, 501-507.

20. Prabla, K., Poddar, A. and Das, s.K. (2014). Marinomonas fungiae sp. nov., isolated from the coral Fungia echinata from the Andaman Sea. International Journal of Systematic and Evolutionary Microbiology, 64, 487-494.

Divya, P. R.

21. Mohitha C., Joy L., Divya P. R., Gopalakrishnan A., Basheer V. S., Koya M. and Jena J. K. (2014). Characterization of microsatellite markers in silver pomfret, Pampus argenteus (Perciformes: Stromateidae) through cross-species amplification and population genetic applications. Journal of Genetics. 93, e89-e93.

22. Divya, P. R., Gopalakrishnan, A., Basheer, V.S., Swaminathan, R., Mohitha, C., Joy, L., Raj Kumar, R., Manoj, P. and Jena J.K. (2014). Population genetic structure of silver pomfret Pampus argenteus along the Indian waters inferred from mitochondrial ATPase 6/8 genes. Mitochondrial DNA 10.3109/19401736.2013.879655.


Gopikrishna, G.

23. Baranski, A.M., Gopikrishna, G., Robinson, N.A., Katneni, V.K., Shekhar, M.S., Shanmugakarthik, J., Jothivel, S., Gopal, C., Ravichandran, P., Kent, M., Arnyasi, M. And Ponniah, A.G. (2014). The Development of a High Density Linkage Map for Black Tiger Shrimp (Penaeus monodon) Based on cSNPs, PlOs One 9(1): e85413. doi:10.1371/ journal.pone.0085413.

24. Robinson, N.A., Gopikrishna, G., Baranski, M., Katneni, V.K., Shekhar, M.S.. Shanmugakarthik, J., Jothivel, S., Gopal, C., Ravichandran, P., Gitterle, T. and Ponniah, A. G. (2014). QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon), BMC Genomics, 15,731 doi:10.1186/1471-2164-15-731.

Goswami, M.

25. Dubey A, Goswami, M, Yadav, K. and Sharma, B.S. (2014). Development and characterization of a cell line WAF from freshwater shark Wallago attu. Molecular Biology Reports 41 (2):915-24.

26. Goswami, M., Sharma, B.S., Yadav, K., Bahuguna, S .N. and Lakra, W.S., 2014a. Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model. Tissue and Cell, 46, 206-212.

27. Goswami, M., Nagpure, N.S. and Jena, J.K. (2014b). Fish Cell Line Repository: an enduring effort for conservation. Current Science 739-739.

Immanuel, G.

28. Sivagnanavelmurugan, M., Karthik Ramnath, G., Jude Thaddaeus, B., Palavesam, A. and Immanuel, G. (2014a). Effect of Sargassum wightii fucoidan on growth and disease resistance to Vibrio parahaemolyticus in Penaeus monodon post-larvae. Aquaculture Nutrition doi: 10.1111/anu.12217.

29. Sivagnanavelmurugan, M., Thaddaeus, B.J., Palavesam, A. and Immanuel, G. (2014b). Dietary effect of Sargassum wightii fucoidan to enhance growth, prophenoloxidase gene expression of Penaeus monodon and immune resistance to Vibrio parahaemolyticus. Fish and Shellfish Immunology, 39, 439 – 449.

30. Navinchandran, M., Iyapparaj, P., Moovendhan, S., Ramasubburayan, R., Prakash, S., Immanuel, G. and Palavesam, A. (2014). Influence of probiotic bacterium Bacillus cereus isolated from the gut of wild shrimp Penaeus monodon in turn as a potent growth promoter and immune enhancer in P. monodon. Fish and Shellfish Immunology, 36, 38 – 45.

Jadhao, A. G.

31. Jadhao, A.G. and Deshpande, K.V. (2014). Sexually dimorphic distribution of calcium- binding protein, calretinin in the preoptic area of the freshwater catfish, Clarias batrachus (Linn.). Neuroscience letters, 579: 86-91.

32. Deshpande, K.V. and Jadhao A.G. (2014). Calcium binding protein calretinin (29 kD) localization in the forebrain of the cichlid fish: An immunohistochemical study. General and Comparative Endocrinology, DOI: 10.1016/j.ygcen.2014.05.027.

33. Biswas, S., Jadhao, A.G., Pinelli, C., Palande, N.V. and Tsutsui, K. (2014). GnIH and GnRH expressions in the central nervous system and pituitary of Indian major carp, Labeo rohita during ontogeny: An immunocytochemical study. General and Comparative Endocrinology, DOI: 10.1016/j.ygcen.2014.06.005.


34. Pinelli, C., Rastogi, R.K., Scandurra, A., Jadhao, A.G., Aria, M., D’Aniello, B. (2014). A comparative cluster analysis of adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry in the brains of amphibians. Journal of Comparative Neurology, 522, 2887–3139.

35. Biswas, S. P., Jadhao, A.G. and Palande, N.V. (2014). Role of catecholamines and nitric oxide on pigment displacement of the chromatophores of freshwater snakehead teleost fish, Channa punctatus. Fish Physiology and Biochemistry, 40, 457-467.

36. Pinelli, C., Jadhao, A.G., Biswas, S.P., Tsutsui, K. and D’Aniello, B. (2014). Neuroanatomical Organization of the Brain Gonadotropin-Inhibitory Hormone and Gonadotropin-Releasing Hormone Systems in the Frog Pelophylax esculentus. Brain, Behavior and Evolution, PMID:25471728.

Kannappan, s.

37. Sivakumar, K., Kannappan, s., Dinesh kumar, M. and Patil, P.K. (2014). Antagonism of marine macro alga Kappaphycus alvarezii extract against luminescence disease causing Vibrio harveyi during Penaeus monodon larviculture. African Journal of Microbiology Research, 8, 458- 469.

38. Sivakumar, K., Kannappan, s., Dinesh kumar, M. and Patil, P.K. (2014). Evaluation of marine macro alga, Ulva fasciata against bio-luminescent causing Vibrio harveyi during Penaeus monodon larviculture. African Journal of Microbiology Research 8, 803 – 813.

Karunasagar, I.

39. Shetty, M., Maiti, B., Venugopal, M.N., Karunasagar, I. and Karunasagar, I. (2014). First isolation and characterization of Edwardsiella tarda from diseased striped catfish, Pangasianodon hypophthalmus (Sauvage). Journal of Fish Disease 37, 265–271.

40. Hamod, M.A., Bhowmick, P.P., Shukur, Y.N., Karunasagar, I. and Karunasagar, I. (2014). Iron shortage and bile salts play a major role in the expression of ompK gene in Vibrio anguillarum. Polish Journal of Microbiology, 63, 27-31.

41. Akhila, D.S, Madhu, M.K, Praveen Rai, Kelly C, Owens, L. and Karunasagar I. (2014). Protection of Litopenaeus vannamei against White Spot Syndrome Virus using bacterially expressed recombinant envelope proteins VP39 and VP28. The Israeli Journal of Aquaculture Bamidgeh, (In Press).

Khedkar, G.D.

42. Khedkar, G.D., Tiknaik, A., Kalyankar, A.D., Chandra Sekhar, A., Khedkar, C.D., Ron, T.B. and Haymer, D. (2014a). Genetic structure of populations and conservation issues relating to an endangered catfish, Clarias batrachus, in India. Mitochondrial DNA, 1-7.

43. Khedkar, G.D., Tiknaik, A.D., Shinde, R.N., Kalyankar, A.D., Ron, T.B. and Haymer, D. (2014b). High rates of substitution of the native catfish Clarias batrachus by Clarias gariepinus in India. Mitochondrial DNA, DOI: 10.3109/19401736.2014.905863.

Mazumder, s.

44. Banerjee, C., Khatri, P., Raman, R., Bhatia, H., Datta, M. and Mazumder, s. (2014). Role of calmodulin-calmodulin kinase II, cAMP/protein kinase A and ERK 1/2 on Aeromonas hydrophila-induced apoptosis of head kidney macrophages. PLoS Pathogens, 10(4), e1004018.

45. Banerjee, C., Singh, A., Das, T.K., Rajagopal, R., Srivastava, A. and Mazumder, s. (2014). Ameliorating ER-stress attenuates Aeromonas hydrophila-induced mitochondrial dysfunctioning and caspase mediated HKM apoptosis in Clarias batrachus. Scientific Reports, 4: 5820.


46. Goswami, R., Mandal, S., Mandal, S., Padhy, P.K., Ray, S. and Mazumder, s., (2014). Effect of temperature and arsenic on Aeromonas hydrophila growth, a modelling approach. Biologia, 69, 825-833.

Mukherjee, J.

47. Mitra, S., Sana, B. and Mukherjee, J. (2014). Ecological roles and biotechnological applications of marine and intertidal microbial biofilms. Advances in Biochemical Engineering and Biotechnology, 146, 163-205.

Muraleedhara, K. G.

48. Mohsin, S., Mahadevan, R. and Muraleedhara, K.G. (2014). Free radical scavenging activity and antioxidant effect of ascophyllan from marine brown algae Padina tetrastromatica. Biomedicine and Preventive Nutrition 4, 75-79.

Riji John, K.

49. Riji John, K., Rosalind George, M., Jeyatha, B., Saravanakumar, R., Sundar, P., Jithendran, K.P. and Olaf Koppang, E., (2014). Isolation and characterization of Indian betanodavirus strain from infected farm-reared Asian seabass Lates calcarifer (Bloch, 1790) juveniles. Aquaculture Research 45, 1481–1488.

50. George, M.R., Riji John, K., Mansoor, M.M., Saravanakumar, R., Sundar, P. and Pradeep, V. (2014). Isolation and characterization of a ranavirus from koi, Cyprinus carpio L., experiencing mass mortalities in India. Journal of Fish Diseases, doi:10.1111/jfd.12246.

sachindra, N. M.

51. Sowmya, R. and sachindra, N.M. (2014). Enhancement of non-specific immune responses in common carp, Cyprinus carpio, by dietary carotenoids obtained from shrimp exoskeleton. Aquaculture Research, doi:10.1111/are.12310.

52. Sowmya, R. and sachindra, N.M. (2014). Protective effect of shrimp carotenoids against ammonia stress in common carp, Cyprinus carpio. Ecotoxicology and environmental safety 107, 207-213.

sahoo, P. K.

53. Das, S., Chhottaray, C., Das Mahapatra, K., Saha, J.N., Baranski, M., Robinson, N. and sahoo, P.K. (2014). Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection. Molecular Biology Reports, 41, 7361-7371.

54. Robinson, N., Baranski, M., Mahapatra, K.D., Saha, J.N., Das, S., Mishra, J., Das, P., Kent, M., Arnyasi, M. and sahoo, P.K. (2014). A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila. BMC Genomics, 15,541.

sahul Hameed, A.s.

55. Abdul Majeed, S., Nambi, K.S.N., Taju, G., Sarath Babu, V., Farook, M.A. and sahul Hameed, A.s. (2014a). Development and characterization of a new gill cell line from air breathing fish Channa striatus (Bloch 1793) and its application in toxicology: Direct comparison to the acute fish toxicity. Chemosphere, 96, 89-98.

56. Abdul Majeed, S., Nambi, K.S.N., Taju, G., Vimal, S., Venkatesan, C. and sahul Hameed, A.s. (2014b). Cytotoxicity, genotoxicity and oxidative stress of malachite green on kidney and gill cell lines of freshwater air breathing fish Channa striata. Environmental Science and Pollution Research, 21, 13539 – 13550.


57. Farook, M.A., Sundar Raj, N., Madan, N., Vimal, S., Abdul Majeed, S., Taju, G., Rajkumar, T., Santhoshkumar, S., Sivakumar, S. and sahul Hameed, A.s., (2014). Immunomodulatory effect of recombinant Macrobrachium rosenbergii nodavirus capsid protein (r-MCP) against white tail disease of giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Aquaculture 433, 395-403.

58. Madan, N., Rajkumar, T., Sundar Raj, N., Farook, M.A., Nambi, K.S.N., Abdul Majeed, S. and sahul Hameed, A.s. (2014). Tissue distribution of Hepatopancreatic Parvo-like Virus (HPV) of shrimp in freshwater rice-field crab, Paratelphusa hydrodomous (Herbst) Journal of Fish Diseases, 37, 969 – 980.

59. Taju, G., Abdul Majeed, S., Nambi, K.S.N. and sahul Hameed, A.s. (2014a). In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita. Comparative Biochemistry and Physiology, Part C, 161, 41-52.

60. Taju, G., Abdul Majeed, S., Nambi, K.S.N., Farook, M.A., Vimal, S. and sahul Hameed, A.s. (2014). In vitro cytotoxic, genotoxic and oxidative stress of Cypermethrin on five fish cell lines. Pesticide Biochemistry and Physiology 113, 15-24.

61. Vimal, S., Madan, N., Farook, M.A., Nambi, K.S.N., Abdul Majeed, S., Rajkumar, T., Venu, S., Thirunavakarasu, A.R. and sahul Hameed, A.s. (2014a). Production of recombinant vaccine using capsid gene of nodavirus to protect Asian sea bass, Lates calcarifier (Bloch, 1790). Aquaculture, 418-419, 148-154.

62. Vimal, S., Abdul Majeed, S., Nambi, K.S.N., Madan, N., Farook, M.A., Venkatesan, C., Taju, G., Venu, S., Subburaj, R., Thirunavakarasu, A.R. and sahul Hameed, A.s. (2014b). Delivery of DNA vaccine using Chitosan-Tripolyphosphate (CS/TPP) nanoparticles in Asian sea bass, Lates calcarifer (Bloch, 1790) for protection against nodavirus infection. Aquaculture, 420-421, 240-246.

santhanam, P

63. santhanam, P., Jothiraj, K., Jeyaraj, N., Jeyanthi, S., Devi, A.S. and Ananth, S. (2014). Effect of monoalgal diet on the growth, survival and egg production in Nannocalanus minor (Copepoda: Calanoida). Indian Journal of Marine Sciences, (In Press).

sarkar U.K

64. Agnihotri, P., sarkar U.K., Nagpure, N. S., Mishra, R.M., Kumar, R., Pal, A., Awasthi, A., Pandey, B.K. and Kumar, N. (2014). Evaluation of reproductive strategies of Silurid fish, Ompok bimaculatus (Bloch, 1794) in six tropical rivers of the Ganges basin. Icthyological Research, (In Press).

65. sarkar U.K., Agnihotri, P., Kumar, R., Awasthi, A., Pandey, B.K., Pal, A., Mishra, R.M., Rawat, Kumar, N. and Singh, S.P. (2014). Comparative assessment of reproductive pattern in butter catfish, Ompok bimaculatus (Bloch, 1794) from 13 different riverine populations in India. Journal of Applied Icthyology, (In Press).

seghal, G.K.

66. Seghal, G.K., A. N. Lipton, S. Priyadharshini, A. Kumar, L. Elizabeth, M. V. Arasu, K. C. Choi, J. Selvin N.A. and Al-Dhabi (2014). Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios, Microbial Cell Factories, 13, 114.

selvin, J.

67. Asha, D., Kiran, G.S. and selvin J. (2014). Biosurfactant production from marine bacteria associated with sponge Callyspongia diffusa. 3Biotech, DOI 10.1007/s13205-014-0242-9.


68. Kiran, G.S., Nishanth, L.A., Priyadharshini, S., Anitha, K. and selvin, J. (2014a). Effect of Fe nanoparticle on growth and glycolipid biosurfactant production under solid state culture by marine Nocardiopsis sp. MSA13A. BMC Biotechnology, 14, 48.

69. Kiran, G.S., Dhasayan, A., Lipton, A.N., selvin, J., Arasu, M.V. and Al-Dhabi, N.A., (2014b). Melanin- templated rapid synthesis of silver nanostructures. Journal of Nanobiotechnology, 12, 18.

70. Kiran, G.S., Sabarathnam, B., Thajuddin, N. and selvin, J. (2014c). Production of Glycolipid Biosurfactant from Sponge-Associated Marine Actinobacterium Brachybacterium paraconglomeratum MSA21. Journal of Surfactants and Detergents, 17, 531-542.

shekhar, M.s.

71. shekhar, M.s. and Ponniah, A.G. (2014). Recent insights into host pathogen interaction in white spot syndrome virus infected penaeid shrimp. Journal of Fish Diseases. DOI:10.1111/jfd.12279.

Vaseeharan, b

72. Shanthi, S. and Vaseeharan, b. (2014). Alpha 2 macroglobulin gene and their expression in response to GFP tagged Vibrio parahaemolyticus and WSSV pathogens in Indian white shrimp Fennoropenaeus indicus. Aquaculture, 418-419, 48-54.

73. Sivakamavalli, J., Selvaraj, C., Singh, S.K. and Vaseeharan, b. (2014). Exploration of protein-protein interacrtion effects on a-2 macroglobulin in an inhibition of serine protease through gene expression and molecular simulations. Journal of Biomolerular Structure and Dynamics, 32, 1841-1854.

All male guppy produced through letrozole treatment


PATeNTs

During the period from 2011 to 2014, five applications have been filed for patents using the research leads obtained from R & D projects sanctioned under Aquaculture and Marine Biotechnology Taskforce. Among the five applications, two were granted while the others are under processing. Dr. Indrani from Mangalore Fisheries College has filed a patent for a consortium of bacteriophages which was granted on 31.03.2014. Dr.Mukherjee from Jadavpur University filed a patent for conico cylinder flask for biofilm cultivation which was granted on 16.10.2014. During the period from 1998 to 2010, two patents have been granted out of seven applications. The details of patents filed during the period from 2010 to 2014 are furnished below:

Indrani Karunasagar

KVAFSU, College of Fisheries, Mangalore-575 002

Patent awarded for “A process of preparing a consortium of bacteriophages useful for controlling luminous bacterial disease in shrimp larvae.’’

Patent No.: 260063 (Indian)

Date of filing: 11-08-2006 Date of Grant: 31-03-2014

Jithendran, K.P.

Central Institute of Brackishwater Aquaculture, Chennai - 600 028

Patent filed for Nested RT-PCR kit for betanodavirus of seabass and not yet granted.

Application No.: 347/DEL/2011 Dt. 11-02-2011

Mukherjee, J.

School of Environmental Studies, Jadavpur University, Kolkata 700 032

Patent granted for “Novel enhanced surface area conico-cylindrical flask (ES-CCF) for biofilm cultivation”

US Patent (Application No.: 13/111,022).

Date of Filing: 19-05-2011 Date of Grant: 16-10-2014

Muraleedhara Kurup, G.

University of Kerala, Trivandrum.

Patent filed for “Biodegradable, biocompatible, wound healing composition” and not yet granted.

Application No.: 840/DEL/2013

Date of Filing: 20-03-2013

sambasiva Rao, K.R.s.

Acharya Nagarjuna University, Nagarjunanagar, A.P.

Patent filed for “Production of a cold active β-Galactosidase from a novel marine psychrophilic Thalassospira Species and an improved process to increase its yield for industrial use thereof”.

File No.: 2772/CHE/2011.


ReseARCH leADs FROM R&D PROJeCTs

During the period from 2011 to 2014, research leads have been obtained by the Principal Investigators in the form of diagnostics, vaccines, cell lines, immunostimulants, bioactive molecules, probiotics etc. from DBT funded R & D projects sanctioned under Aquaculture and Marine Biotechnology Taskforce. Fourteen PIs have obtained research leads which have to be taken up for product development for the benefit of industry. The details of research leads obtained by PIs have been summarized below:

Aparna Dixit, School of Biotechnology, Jawaharlal Nehru University, New Delhi

• The rOmpR and rOmpC as vaccine candidates against a fish bacterial pathogen, Aermonas hydrophila.

• A novel adjuvant capable of enhancing immune response in Labeo rohita.

• Generation of polyclonal antisera as a detection tool for cytokines and other innate immune response genes in L. rohita.

Dinkar sahal, ICGEB, New Delhi – 110067

E-Mail: [email protected]

• Antiplasmodial molecules from marine organisms

Felix, s., Tamilnadu Fisheries University, Ponnari Centre, Chennai

• Marine Single Cell Detritus (MSCD) - a ‘Biofeed’ for larval and post-larval stages in shrimp hatcheries.

Gnanamani, A., CLRI, Adyar, Chennai – 600020

Email: [email protected]

• A process has been developed for production of a non-ionic biosurfactant for industrial application from a marine microbe. Patented (DEL 1261/2010).

• A process for production of bioadhesive from fatty acids has been developed and the process has been patented (DEL 2067/2010)

Indrani Karunasagar, Department of Fishery Microbiology

KVAFSU, College of Fisheries, Mangalore-575 002.


• Five novel OMP vaccine molecules against Aeromonas infection and edwardsiellosis.

• PCR and dot blot assay for detection of Aeromonas species and Edwardsiella tarda

• Omp based recombinant vaccine and a whole cell killed vaccine for Vibrio anguillarum

• A multiplex PCR for detection of WSSV, HPV, MBV, IHHNV of shrimps.

• Monoclonal antibody based kit for farm level level diagnosis for WSSV and PmDNV

• Diagnostic kits for Vibrio parahemolyticus and Salmonella.

• dsRNA specific to WSSV, PmDNV, MrNV and XSV.

• Bacteriophages for controlling luminous bacterial disease in shrimp larvae (Patent No.: 260063 dated 31.03.2014).


Jeyasekaran, G., College of Fisheries, Thoothukudi - 628 008, Tamilnadu

• Multiplex PCR for simultaneous detection of Salmonella, Vibrio cholerae and E. coli from fish and fishery products.

Jeya shakila, R., College of Fisheries, Thoothukudi - 628 008, Tamilnadu

• Technology developed for the formation gelatin multicomposite films, gelatin antimicrobial films, gelatin scaffolds, collagen films and collagen scaffolds from fish source (Fig. 5).

Jithendran, K.P., CIBA, Chennai


• RT-PCR kit for beta nodavirus

Mukherjee, J., Jadavpur University, Kolkata - 700 032

E-mail: [email protected]

• A novel polymethylmethacrylate conico-cylindrical flask for production of bacterial and fungal biofilm (Fig. 4). US Patent (Application No.: 13/111,022 dated 16.10.2014)

Muraleedhara Kurup, G., Dept. of Biochemistry, University of Kerala, Thiruvananthapuram -695581

Email ID: [email protected]

• A biodegradable and biocompatible wound healing composition from shrimp waste.

• Indian patent (No: 50/2014 dated 12/12/2014) (Fig. 6).

Rosamma Philip, Cochin University of Science and Technology, Cochin – 682016


• Bio-granules of actinomycestes as bioremediators and as bio-control agents of vibrios in shrimp larval production systems.

sahul Hameed, A.s., OIE Reference Laboratory, C.Abdul Hakeem College, Melvisharam – 632509, Vellore Dt., TN


• In vivo synthesis of dsRNA specific to WSSV of shrimp to control WSSV infection (Field evaluation and product development will be carried out in collaboration with GPS Biotech, Hyderabad. Financial assistance has been sanctioned by BIRAC under SBIRI scheme. KoVax Ltd., an Israel based aquaculture company showed interest to commercialize this product globally).

• Recombinant viral immunostimulants (WSSV, HPV, IHHNV and MrNV) for shrimp and prawn to enhance resistance in shrimp (GPS Biotech, Hyderabad showed interest to commercialize these products).

• Whole cell, recombinant and DNA vaccines against fish nodavirus and Vibrio anguillarum.

• Field level nanoparticle based immunodiagnostics for viral pathogens of shrimp and prawn (GPS Biotech, Hyderabad showed interest to commercialize these products).

• Twenty five cell lines from a variety of marine, brackishwater and freshwater fish species (Seventeen fish cell lines have been deposited in NBFGR, Lucknow) (Fig. 9).


Riji John, College of Fisheries, Thoothukudi - 628 008, Tamilnadu


• Four cell lines developed from different tissues of clownfish tissues (CFFN2, CFCP1, CFSP and CFBR) and deposited at National Repository for Fish Cell lines at NBFGR, Lucknow (Fig. 7).

sobhana, K.s., Central Marine Fisheries Research Institute, Cochin

E-mail: [email protected]

• Eighteen cell lines from four species of marine fishes deposited in the National Repository of Fish cell lines (NRFC), NBFGR, Lucknow for scientific community (Fig. 8).

Field trial carried out in shrimp farm using ‘Immuzone’


Gelatin and collagen scaffold Collagen films

Antimicrobial gelatin films

Fig. 5 Products developed by Dr.R.Jeya shakila from fish source

Fig. 6 Photograph showing the wound healing in rat using biocomposite prepared from shrimp waste by Dr.G. Muraleedhara Kurup

Fig. 4 Rotating Disc bioreactor developed by Dr.Joydeep Mukherjee for production of bacterial and fungal biofilm


Fig. 7 Cell lines developed from clown fish by Dr.Riji John

Fig. 8 Cell lines developed from marine fish by Dr.K.s. sobhana

Fig. 9 Cell lines developed from different species of fish by Dr.A.s.sahul Hameed


Ph.D. AWARDeD

One of the objectives of the DBT is to develop manpower in multidisciplinary areas of biotechnology and also to encourage researchers to join as JRF and SRF in the R&D projects leading to doctoral programme. The total number of Ph.Ds produced in DBT funded R & D projects sanctioned under Aquaculture and Marine Biotechnology Taskforce is 35 during the period from 2011 to 2014 (Table 1). Among the 86 PIs, 17 PIs have produced 35 Ph.D. candidates. The number of Ph.D. candidates awarded in 2011 was 3 and it gradually increased to 9 in 2012, 9 in 2013 and 14 in 2014. Dr.Indrani has produced 9 Ph.D. candidates followed by Dr.A.S.Sahul Hameed (05).

Table 1 list of Ph.D. awarded during 2011–2014

s.No.

Name of PI who guided

Name of the student Title of the Thesis

Year of

award

Name of theUniversity

1. Aparna Dixit Y. Sunitha Kumari

Immunogenic and protective potential of recombinant outer membrane proteins OmpF and OmpC, members of two-component regulatory system of Aeromonas hydrophila

2014Jawaharlal Nehru University, New Delhi.

2. barat, A. M. Rakesh

Development and characterization of microsatellite markers in Garra gotyla Family: Cyprinidae, Pisces

2013Kumaun University, Nainital

3. barat, A. C. Suresh

Population genetic structure of the snowtrout Schizothorax richardsonii (gray) using microsatellite markers

2013Kumaun University, Nainital

4. basavaraja, N.

B.H. Chandrashekhara.

Production of an all- male populationof guppy, Poecilia reticulata (Robert John Lechmere 1866) through application of aromatase inhibitor

2011

Karnataka Veterinary Animal & Fisheries Sciences University

5. basavaraja, N. K.M. Bhagwan

Masculinization of Siamese fighting fish, Betta splendens (Regan, 1910) through the application of letrozole and norethindrone

2012


6. baskar, N. A.K. Rai

Biotechnological approaches for recovering lipids from fish processing byproducts and biological activity of recovered lipids

2013 University of Mysore


7. Chiplunkar s.V B.Babita

Evaluation of Extracts of some marine mollusks on Bone Metabolism: An Experimental Study

2011 Mumbai University

8. Das, P. L. SahooDevelopment of selected genomic resources in Indian major carp, Labeo rohita

2014Orissa University of Agriculture and Technology

9. Immanuvel, G. M. Sivagnana-velmurugan

The effect of seaweed polysaccharides on immune activity and immune gene expression in shrimp Penaeus monodon culture system

2014

Manonmaniam Sundaranar University

10. Indrani Karunasagar B.Maiti

Molecular and immunological studies of outer membrane proteins of Aeromonas and Edwardsiella spp

2012 Mangalore University

11. Indrani Karunasagar M. Shetty

Development and delivery of immune antigens for protection against edwardsiellosis and motile aeromonad disease in finfish

2012


12. Indrani Karunasagar M. Neema

Bioinformatics based study of outer membrane proteins in the fish pathogen Edwardsiella spp.


13. Indrani Karunasagar D.Devanand

Study of outer membrane proteins (OMPs) of Salmonella spp. and development of OMP and virulence based rapid diagnostics for food and clinical samples.

2012 Manipal University

14. Indrani Karunasagar M.A. Hamod

Development of recombinant protein vaccine against Vibrio infection of aquatic animals. 2012


15. Indrani Karunasagar P. Rai

Recombinant protein and double stranded RNA (dsRNA) as immune molecule against infectious hypodermal and hematopoietic necrosis virus (IHHNV) infection of shrimp

2013


16. Indrani Karunasagar P.P.Bhowmick

Molecular genetic characterization of pathogenicity islands SPI-II and SPI-III of Salmonella strains associated with seafoods.



17. Indrani Karunasagar M.P.Safeena

Efficacy of recombinant protein and double stranded RNA (dsRNA) for the protection of shrimps against hepatopancreatic parvovirus (HPV),

2014


18. Indrani Karunasagar D.S.Akhila

Development of recombinant protein and RNAi to protect shrimp from white spot syndrome virus (WSSV) infection

2014


19. Kannappan, s. K. Sivakumar

Efficacy of marine algae against quorum sensing bioluminescence causing Vibrio harveyi,

2014 University of Madras

20. Khedkar, G.D. T.Anita

DNA microsatellite marker based studies of catfish, Clarias batrachus populations in India and its strategic interventions in breeding program

2014

Dr.Ambedkar Marathwada University, Aurangabad

21. Muralidharan, K.G. M. Sulaiman Marine Biopolymer composites

and their applications 2013 University of Kerala

22. sahul Hameed, A.s. V. Sarath Babu

Application of fish cell lines for nodaviral propagation and development of diagnostics and vaccine

2011Thiruvalluvar University, Vellore, TN

23. sahul Hameed, A.s. G.Taju

Application of fish cell lines for toxicological studies: an alternative to fish.


24. sahul Hameed, A.s. N.Sunder Raj

Development of an in vitro model for replication of white spot syndrome virus (WSSV) of shrimp and immune gene expression in shrimp.


25. sahul Hameed, A.s. N. Madan

Study on Penaeus monodon densovirus (PmDNV) of shrimp 2014

Thiruvalluvar University, Vellore, TN

26. sahul Hameed, A.s. S.Vimal

Development and protective efficacy of recombinant and DNA vaccines against fish nodavirus.


27. sanil, G. V. SreejaDiversity of cephalopods (Mollusca: Cephalopoda) of Kerala coast

2014 University of Kerala


28. santhanam, P. N. Jeyaraj

Studies on biodiversity, experimental biology, hatchery production and suitability of marine copepod Paracalanus parvus as live feed for larval rearing of Asian seabass L.calcarifer and tiger shrimp P.monodon.

2012 Bharathidasan University, TN

29. santhanam, P. K. Jothiraj

Studies on biology, culture and efficacy of marine copepod Nannocalanus minor for fish larviculture.


30. santhanam, P. M. Kathiresan

Composition and community structure of plankton from Muthupet coastal waters and application of marine copepod Oithona rigida for larval rearing of Pacific white shrimp Litopenaeus vannamei.


31. sarkar, U.K. A. Praveen

Population structure and reproductive biology of Ompok bimaculatus in the tributaries of river Ganges basin in India

2014 A.P.S. University, Rewa

32. sarkar, U.K. B.K.Pandey

Pursuing, Characterization of different wild populations of silurid butter catfish, Ompok bimaculatus (Bloch, 1794), using molecular marker

2014 U.P. Technical University

33. sarkar, U.K. A. Rawat

Pursuing, a Study to analyze reproductive potency of buttercatfish Ompok bimaculatus (Bloch 1794) and its induced breeding

2014

34. sarita bhat K. Sapna

Isolation, purification, characterization and application of proteinaceous protease inhibitor from marine bacterium Pseudomonas mendocina BTMW 301

2013

Cochin University of Science and Technology

35. Vaseeharan, b. S. Shanthi

Molecular cloning, characterization and expression analysis of serine proteinase, Penaeidins and Alpha 2.

2014 Alagappa University, TN


PlACeMeNT DeTAIls OF JRF/sRF APPOINTeD IN DbT-sPONsOReD PROJeCTs

The JRF/SRFs appointed in DBT-funded projects have been placed in different organizations under different categories in India and abroad. The placements include teaching and research. The details of placement of JRF/SRFs are given below:

s.No. Name of PI Name of the JRF Placement details

1. s.V.Alavandi Satheesh Kumar Scientist, National Institute of Epidemiology, Chennai

2. Aparna Dixit Sunita Kumari, Y.Assistant Professor, Aurobindo College, University of Delhi

3. barat, A. Neetu Shahi ARS Scientist, ICAR Directorate of Coldwater Fisheries Research, Bhimtal

4. basavaraja, N. Nitya, N.B. Faculty, Sher-e-Kashmir University of Agricultural Sciences and Technology, J & K

6. baskar, N. Amit Kumar Rai Scientist – B, IBSD, Ganktok, Sikkim

7. Chiplunkar, s.V. JRF PDF (UGC-DS Kothari Fellowship)

8. Das, P. Alok Patel Assistant Professor, University of Chile, Santiago, Chile

9. Goswami, M. Bhagawati, S.S. Institute of Biomedical Sciences, Taipei, Taiwan

10. Indrani, I.

Praveen Rai Assistant Professor, Nitte University, Mangalore.

Deekshit Assistant Professor, Nitte University, Mangalore.

Krishna Kumar Assistant Professor, Nitte University, Mangalore.

Akhila Lecturer, Shri Dharmasthala Manjunatheshwara College, Ujire

Maiti, B. Assistant Professor, Nitte University, Mangalore.

Suresh, T Assistant Professor, College of Fisheries, Mangalore

Anuj, T. Veterinary & Animal Science University, Punjab

Divu, D. ARS Scientist, CMFRI

Surendranath, S.V. Assistant Professor (contract), College of Fisheries, Mangalore

Devanand, D. Assistant Professor, J.S.S Medical College, Mysore.

Safeena, M.P. Assistant Professor, Kerala University of Fisheries & Ocean Studies, Ernakulam


Mohan, S.A. Assistant Director of Fisheries, Bangalore

Vijay Atnur Assistant Professor, Karnataka Veterinary, Animal & Fisheries Sciences University, Bijapur

Naveen Kumar, S. Research Scientist, Dammam

Umesh, K.R. Agriculture Officer in Syndicate Bank

11. Jithendran, K.P. SRF Assistant Professor, Sacred Hearts College, Cochin

12. Kannappan, s. Sivakumar, K. Sub-Inspector of Fisheries, Department of Fisheries, Tamilnadu State Government.

13. Muraleedhara, K.G. Amol, K. Fish Biologist, Dr.B.Ambedkar Marathwada University, Aurangabad.

14. Pandian, s.K. Nithyanand, P. PDF, Negev Ben Gurion University, Israel

15. Rajendran, K.V. Reena, Y., Smruti, M. Scientific Officer, SRL Diagnostics, Mumbai

16. sahul Hameed, A.s.

Nazeer Basha, A. Assistant Professor, C.Abdul Hakeem College, Melvisharam, TN

Balasubramanian, G. Assistant Professor, A.Anna Govt. Arts College, Cheyyar, Tamilnadu

Madan, N. Research Scientist, GPS Biotech, Hyderabad. .

Syed Musthaq PDF, National University of Singapore

Vimal, S. Research Scientist, GPS Biotech, Hyderabad..

Sunder Raj, N. PDF, Agricultural Research Organization, Israel.

S.Rajesh Kumar PDF, National University of Singapore

V.P. Ishaq Ahmed University of British Columbia - Vancouver, BC, Canada.

Sarathi, S. Lakehead University, Thunder Bay Campus, Canada.

Venkatesh, C. Associate Professor, Dr. N.G.P. Arts and Science College, Coimbatore

Ravi, N. Scientist, Sathyabhama University, Chennai

Parameswaran, V. Scientist, Sathyabhama University, Chennai

Sudhakaran, R. Assistant Professor, VIT University, Vellore, Tamilnadu

John Thomas Assistant Professor, VIT University, Vellore, Tamilnadu

17. santhanam, P. N. Jeyaraj PDF (UGC-DS Kothari Fellowship)

18. sarita bhat Sapna, K. Assistant professor, CUSAT, Cochin - 22

19. sarkar, U.K. Bipin, C.P. Lecturer, National PG College, Lucknow

20. Thajuddin, N.Mubarak Ali, D.

Praveen Kumar

PDF, Incheon National University, South KoreaPDF, Korea Institute of Energy & Research, South Korea


PRODUCTs AVAIlAble IN THe MARKeT FROM DbT-FUNDeD PROJeCTs

Researchers supported thorugh DBT sponsored R & D projects have significantly developed good products and have been transferred the technology to industry for commercialization. The products available in the market for the benefit of shrimp farmers and hatchery operators are summarized below:

Product - 1

Brand Name of the Product: AMPlI-WssV (Detection of WssV of shrimp)

Developed by Dr.I.Karunasagar and Dr.Indrani Karunasagar, College of Fisheries, Mangalore

Marketed by Mangalore Biotech Laboratory, Mangalore

Purpose: AMPLI-WSSV is the kit designed to detect very low levels of WSSV in shrimp by PCR method. It is suitable for detection of WSSV in animals, water, sediments or any other source.

Product - 2

Brand Name of the Product: lUMI – NIl Mbl



Purpose: This product is useful for controlling luminous bacterial disease in shrimp hatcheries. The product is a consortium of novel bacteriophages. It can be used for preventing as well as treating luminous Vibriosis caused by Vibrio harveyi and can be a good alternative to antibiotics in aquaculture


Product - 3

Brand Name of the Product: AQUAsTIM - Mbl



Purpose: Stimulating the immune response of shrimp by using bacterial and yeast products. Administration of Aquastim MBL by immersion or through feed stimulates the immune system of shrimp.

Product - 4

Product Name: PCR kit for Vbrio harveyi



Purpose: This kit is useful in assessing the quality of Penaeus monodon larvae and for monitoring eggs, larvae and hatchery water for the presence of V. harveyi.

Product - 5

Product Name: Nitrifying bioreactor for Re-circulating Aquaculture system

Designed by Dr.Bright Singh, Cochin University of Science and Technology, Kochi

Marketed by Oriental Aquamarine Biotech India (Pvt.) Ltd., Coimbatore

Purpose: The reactor is proposed for building up Recirculating Aquaculture Systems (RAS) where total removal of organic matter with the help of an indigenous bioaugmentor preparation ‘DetrodigestTM’ and elimination of ammonia to elemental nitrogen by the nitrifying bacterial consortia immobilized on the cartridges of the reactors are accomplished.


Product - 6

Product Name: Dr.sahul’s PCR kit

Developed by Dr.A.S.Sahul Hameed, OIE Reference Laboratory, C.Abdul Hakeem College, Melvisharam – 632509, Vellore TN.

Marketed by Poseidon Biotech, Chennai

Purpose: This kit is useful to detect shrimp viruses such as WSSV, IHHNV, MBV and HPV. This kit has been distributed not only in India and also other countries like Tanzania, Sri Lanka etc. Till date, the Company has sold more than 600 kits worth about Rs.60,00,000 (equivalent to the amount of Rs.1,28,00,000 for import kits).

Product - 7

Product Name: Immuzone

Developed by Dr.A.S.Sahul Hameed, OIE Reference Laboratory, C.Abdul Hakeem College, Melvisharam – 632509, Vellore TN.

Marketed by Poseidon Biotech, Chennai

Purpose: IMMUZONE is derived from an Indian medicinal plant and contains a group of compounds which enhance the immunity of shrimp against viral infections. It does not contain Glucans and Mannans. The company has sold more than 5,000 litres of this product worth about Rs.1,00,00,000.


ReFReNCes

Banerjee, C., Khatri, P., Raman, R., Bhatia, H., Datta, M. and Mazumder, s. (2014). Role of calmodulin-calmodulin kinase II, cAMP/protein kinase A and ERK 1/2 on Aeromonas hydrophila-induced apoptosis of head kidney macrophages. PLoS Pathogens, 10(4), e1004018.

Ramasamy, K., Ezhilrani, R., Vinitha, K. and Jayaraman, R., 2014. “Indian research contributions in the Aquaculture journal during 1972 – 2011: a Scientometric Study”. Library Philosophy and Practice (e-journal). paper 1185. http://digitalcommons.unl.edu/libphilprac/1185

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Our Guiding Force Task Force Committee Members

1. Dr.Dilip Kumar Chairman

2. Dr.Iddya Karunasagar Co-Chairman & Member

3. Dr.H.Devaraj Member

4. Dr.W.s.lakra Member

5. Dr.s.Felix Member

6. Dr.P.A.loka bharathi Member

7. Dr.Aparna Dutta Gupta Member

8. Dr.R.Kirubagaran Member

9. Dr.A.s.sahul Hameed Member

10. Dr.P.K.sahoo Member

11. shri.Y.C.Thampi sam Raj Member

12. Dr.Kartik shanker Member

13. Dr.I.s.bright singh Member

14. Dr.Pawan K. Dhar Member

small-scale production of ‘Immuzone’ in lab for field trial

Documents

Compendium - Department of Biotechnology · This compendium has been distributed to different parts of the country among the researchers and was found to be useful to the scientists