Compound Light Microscope

• Has two lenses• Light must pass through object to be seen

Eyepiece

•Maginifies 10X

•Do Not Touch

•Clean with lens paper only

Body tube

Rotates to move eyepiece

Revolving Nosepiece

Turns to changes objectives

High Power Objective

Magnifies 40X

Total magnification 400X

Low Power Objective

Magnifies 4x

Total 40X

Medium Power

Magnifies 10X

Total 100X

Stage

Place slide to be viewed

Diaphragm-disc under stage

Changes the amount of light passing through specimen

Base

Place hand under base to hold or carry

Coarse Adjustment

Moves stage to focus

Never use when on High Power

Fine Adjustment

Moves stage small amount to focus

Arm

Hold to carry microscope

Rules for using the Microscope

• Never touch lenses• Use lens paper only to clean lenses

(eyepiece and objectives)• Carry with two hands, one on arm and the

other under the base• Never use coarse adjustment with high

power objective

Important Words To Know• Specimen-the object or organism you are

going to look at through the microscope• Slide-rectangle piece of glass to put your

specimen on• Cover glass-glass square you put over the

specimen• focus-to make clear• Field of view- the circle of light you see

when looking through the microscope

Making a slide

• Place specimen in center of slide• Place drop of water over specimen• Place cover slip over specimen at an angle

Microscope Procedure

• Start with low power• Focus with coarse adjustment, make final

adjustment with fine adjustment• Center object in field of view• Change to medium power• Focus and center object• Change to high power• Focus with fine adjustment only