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A

Supplementary information, Figure S2 Validation of cell migration inhibitors.

A. NRK cells stably expressing TSPAN4-GFP were transfected with NC (control) or

Sharpin siRNA. Cells were cultured for 12 h and observed by time-lapse confocal

microscopy. Cells were assessed for migration speed. n=100 cells from three

independent experiments. Error bars indicate the s.d. B. TSPAN4-GFP-expressing

NRK cells were treated with or without Blebbistatin and Dynasore and observed by

time-lapse confocal microscopy. Cells were assessed for migration speed. n=30 cells

from three independent experiments. Error bars indicate the s.d. NC, control cells

(untreated); BS, + Blebbistatin; DS, + Dynasore.