Electrophysiology & Live-Cell Imaging

Electrophysiology & Live-Cell Imaging

chambers • perfusion • temperature controlpatch clamp and oocyte amplifier

www.warneronline.com

This brochure will guide you through the process of selecting the components needed to build a rig for electrophysiological recordings or imaging. All Warner Instruments products are designed to provide quality working conditions when low electrical noise is a requirement.

This brochure does not contain every relevant product we carry, so we invite you to visit our website at www.warneronline.com for the complete catalog. We are happy to assist in finding the right instruments for the planned application and to assemble the best suitable products for a complete rig.

Warner Instruments provides world-class customer support, free of charge.

Warner Instruments offers a range of amplifiers optimized for many electrophysiological applications, including:

• Patch clamp

• Oocyte clamp

• Extracellular

• Intracellular

• Bilayer

• Epithelial transport (Ussing)

This brochure focuses on patch and oocyte clamp applications. Please see our website for other applications.

Introduction

Table of Contents

Recording ................................................................. 2

Patch-Clamp Recording .......................................... 2

Oocyte Recording ....................................... 4

Acquisition Hardware & Software .............. 5

Recording and Imaging Chambers ........................ 7

Introduction ................................................ 7

Overview .................................................................. 8

Finding Chambers by Application ............. 10

Closed Bath Chambers ............................. 11

Open Bath Chambers ............................... 11

Quick Release Chambers ......................... 12

Field Stimulation Chambers ...................... 12

Perfusion Inserts for 35 mm Dishes ......... 13

Platforms .................................................. 14

Chamber Accessories ............................................ 15

Temperature Control Systems ............................. 16

Background ............................................... 16

Temperature Controllers ........................... 16

Temperature Control Accessories ....................... 17

Perfusion Control ................................................... 19

Background ............................................................ 19

Perfusion Systems ................................................. 19

Waste Systems ........................................ 20

Perfusion Accessories ........................................... 21

Stage ....................................................................... 21

Micromanipulation ................................................ 22

Pipette Puller .......................................................... 22

Capillary Glass ....................................................... 23

Microinjection ........................................................ 23

Automated Pipette-based Patch-Clamp .............. 24

Scientific Meetings ................................................ 25

Patch C

lamp, O

ocyte and Live-Cell Im

aging

Warner Instruments Inc. • phone (203) 776 .0664 • to l l f ree U.S. (800) 599 .4203 • fax (203) 776 .1278 • www.warneronl ine.com 1

Temperature ControllersAvailable in dual and single channel versions for heat only and peltier driven peripherals. All temperature controllers use ultra low noise power supplies for sensitive electrophysiology recording.

Valve ControllersOur perfusion valve control systems are robust, easy to operate, and designed to control six or eight valves. There are several types of valves to choose from. Gravity or pressure driven systems available.

Recording Chambers and Slice AnchorsOur chamber selection guide, available online and in our print catalog, is useful for choosing the best chamber for your experiment. The guide lists chambers by application, bath type, bath volume, and coverslip size.

Stage AdaptersStage adapters are available for all major microscopes and stages currently on the market.

Solution HeatersIn-line solution heaters are a simple and effective way to warm solutions flowing into Series 20 Chambers.

An Integrated Systems Approach

The Complete Rig

Heated PlatformsPlatforms function as the base for Series 20 Chambers and provide clamping to make a seal between the chamber and coverslip.

Our expert application scientists will consult with you to identify the best components needed to assemble a complete rig.

2 Warner Instruments Inc. • phone (203) 776 .0664 • to l l f ree U.S. (800) 599 .4203 • fax (203) 776 .1278 • www.warneronl ine.com

HEKA EPC 10 USB Amplifier and PATCHMASTERWarner Instruments can now provide the gold standard in the field of Patch-Clamp amplifiers, the HEKA EPC 10 USB. Combining it with our amplifier control, acquisition and analysis software, PATCHMASTER, it creates a complete Patch-Clamp system that allows data generation from a variety of recording configurations:

• Whole-Cell recordings (conventional and perforated)

• Single-Channel recordings

• Bilayer recordings

• Loose Patch recordings

• Intracellular voltage recordings with high resistance electrodes

• And many more

The main technical features of the EPC 10 USB which make it a unique instrument include:

• Integrated acquisition board for low noise and easy setup

• Fully computer-controlled; allows complete automation of experiments

• Optional small S-Probe headstage, only 49 x 17 x 14.5 mm, 25 g

• Optional bath sense for amperometry

• 1–4 headstages

• Linking two instruments supports up to 8 headstages

• 3 feedback resistors per headstage

• Onsite, user-performed calibration of headstage

EPC 10 USB

S-Probe

Selected Specifications

Amplification range 0.005 mV/pA up to 2000 mV/pA

Holding potential Up to ± 2V

Bandwidth Up to 100 KHz

Noise in medium gain range including acquisition board

Up to 1 kHz: ~180 fA rms (theoretical limit) Up to 3 kHz: ~ 320 fA rms (theoretical limit) Up to 10 kHz: ~ 580 fA rms

Noise in high gain range including acquisition board

Up to 1 kHz: ~31 fA rms Up to 3 kHz: ~72 fA rms Up to 10 kHz: ~350 fA rm

Filter 1 10KHz, 30 KHz, 100 KHz using 6 pole Bessel and HQ

Filter 2 100 Hz – 15 KHz using 4-pole Bessel or Butterworth

Current range Low gain: ± 2 µA Medium gain: ± 20 nA High gain: ± 200 pA

Digital IO 16 digital outputs and 16 input channels including trigger

Analog IO Up to 5 analog input channels, up to 3 output channels

To ensure full functionality, the EPC 10 USB computer-controlled amplifier and acquisition system has to be combined with the appropriate software. Please read about PATCHMASTER in the Acquisition and Software section. Upon user request, we can provide a direct link library (dll) that allows the use of custom-made software (e.g., Matlab® or C) to control the hardware.

Order # Product

89-5000 EPC 10 USB patch clamp amplifier, Single

89-5001 EPC 10 USB patch clamp amplifier, Double

89-5002 EPC 10 USB patch clamp amplifier, Triple

89-5003 EPC 10 USB patch clamp amplifier, Quadro

89-5137 S-Probe headstage

89-5138 S-Probe upgrade, upgrade from normal headstage

Recording

Patch-Clamp Recording

Patch C

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aging


PC-505B PC-501A


Amplification range 0.05 mV/pA up to 1000 mV/pA, depending on headstage

Holding potential Up to ± 200 mV

Bandwidth 25 KHz

Noise Measured with 8-pole Bessel filter, open input, 50 GΩ resistor Up to 1 kHz: ~0.035 pA RMS Up to 5 kHz: ~0.150 pA RMS

Stimulus range in VC Up to 1 V, depending on command sensitivity

Stimulus range in CC Up to 1 nA, depending on command sensitivity

Telegraphing output Gain, filter and mode (VC/CC) setting



Amplification range 0.1 mV/pA up to 1000 mV/pA, depending on headstage


Bandwidth 10 KHz

Noise Measured with 8-pole Bessel filter, open input, 10 GΩ resistor Up to 1 kHz: ~0.06 pA RMS Up to 5 kHz: ~0.2 pA RMS Up to 10 kHz: ~0.4 pA RMS

Stimulus range in VC Up to 1 V, depending on command sensitivity

Stimulus range in CC Up to 1 nA, depending on command sensitivity

Telegraphing output Gain


Warner PC-505BThe PC-505B is a manually controlled patch clamp amplifier that provides telegraphing outputs and command inputs to accommodate data acquisition systems. This amplifier is well suited for performing both patch clamp and whole cell studies. It is also capable of making bilayer recordings, but we recommend the more specialized BC-535 for this purpose. The headstage provides both high and low gain feedback resistors which support a wide gain and current range.

Features include:

• Switchable feedback-resistor based headstage

• CSlow and CFast compensation

• Series R compensation

• Voltage offset compensation

• Leak subtraction

Warner PC-501ASimilar to the PC-505B, the PC-501A is a manually controlled patch clamp amplifier that provides telegraphing outputs and command inputs to accommodate data acquisition systems. This amplifier has slightly more relaxed specifications and is lower priced, making it optimal for teaching purposes and for research labs doing more general measurements.

As with the PC-505B, the PC-501A is well suited for performing whole cell studies and single channel recordings. It is also capable of accommodating bilayer studies.

Features include:

• Voltage clamp as well as current clamp

• CSlow and CFast compensation

• Voltage offset compensation

• Leak subtraction

Order # Product

64-0000 PC-505B with LC201B headstage (50 GΩ/500 MΩ)

64-0001 PC-505B with HC-202B headstage (50 GΩ/50 MΩ)

64-0002 PC-505B with HB-205B bilayer headstage (50 GΩ/500 MΩ)

Order # Product

64-0007 PC-501A with 10 GΩ headstage

64-0008 PC-501A with 1 GΩ headstage

64-0009 PC-501A with 100 MΩ headstage

64-0010 PC-501A with 10 GΩ headstage for bilayer studies

64-0012 PC-501A with 1 GΩ headstage for voltammetry

64-0013 PC-501A with 100 MΩ headstage for voltammetry


EPC 800 USB

OC-725C

Amplifier, Acquisition and Software BundlesWe offer bundles of Warner amplifiers, HEKA acquisition hardware LIH 8+8 and PATCHMASTER software. PATCHMASTER and the LIH 8+8 are described in the Acquisition Hardware and Software section.

Recording

HEKA EPC 800 USBThe EPC 800 USB is a hybrid amplifier that can either be operated remotely with the appropriate HEKA software or the dll (similar to the EPC 10 USB) or in manual mode using the front panel controls. In contrast to other manually controlled amplifiers, the EPC 800 USB provides front panel controls, automatic compensation of pipette capacitance, cell capacitance and serial resistance as well as all offsets. The EPC 800 USB technology is similar to the EPC 10 USB, but it does not include a built-in data acquisition board. Therefore it can be paired with any data acquisition system suitable for patch clamp recordings.


Amplification range 0.005 mV/pA up to 2000 mV/pA

Holding potential Up to ± 1V

Bandwidth Up to 100 KHz

Noise in medium gain range including acquisition board

(measured with an open input, 8-pole Bessel filter including acquisition board and 50 GΩ resistor) DC to 1 kHz < 0.03 pA RMS DC to 3 kHz < 0.08 pA RMS DC to 10 kHz < 0.225 pA RMS

Filter 1+2 6 pole + 4 pole Bessel, 100 Hz-100 KHz


Telegraphing output Gain, Filter, CSlow, Mode (VC/CC)


Amplification range 0.001 up to 100 V/µA

Holding potential up to ± 200 mV

Bandwidth 10 KHz

Compliance Voltage ± 180 V

Filter output 1 KHz, 4 pole Bessel

Order # Product

89-5004 EPC 800 USB patch clamp amplifier, Single

89-5138 S-Probe upgrade from normal headstage

Order # Product

64-0028 OC-725C Oocyte amplifier

64-0030 Optional differential headstage for OC-725C

Order # Product

64-2362 PC-501 with 10 G Ohm headstage, PATCHMASTER and LIH 8+8

64-2363 PC-501 with 1 G Ohm headstage, PATCHMASTER and LIH 8+8

64-2364 PC-501 with 100 M Ohm headstage, PATCHMASTER and LIH 8+8

Warner OC-725CThis amplifier is the industry standard for oocyte clamping. A differential headstage is available separately.

The amplifier features:

• Unique bath clamp circuitry which does not require series resistance compensation

• Two clamp speeds for small and fast response time

Oocyte Recording

Patch C

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aging


iTEV 90 USB

TEV-700

Warner TEV-700This system is designed to provide the necessary components needed to perform traditional oocyte recording. The system is comprised of an OC-725C, RC-3Z chamber and two micromanipulators. A microscope, cold light source, and baseplate are recommended. This system also requires separate data acquisition.

HEKA LIH 8+8The HEKA EPC 10 USB and iTEV 90 USB amplifiers feature an integrated acquisition board. All other HEKA and Warner amplifiers need an acquisition instrument for recording and stimulation. The HEKA LIH 8+8 acquisition system samples with up to 200 KHz. Two instruments can be linked. The LIH 8+8 connects to a Windows PC or Apple OS computer using a USB 2.0 port.

LIH 8+8


Amplification range 10 up to 10000 mV/µA


Holding current ± 120 µA

Compliance Voltage ± 120 V

Current Filter Up to 20 KHz

Clamp gain 10 to 500


Digital IO TTL 3.3V and 5V compatible

16 digital outputs and 16 input channels including trigger

Analog Inputs 8 channels, ± 10 V

Analog Outputs 4 channels, ± 10 V

Order # Product

64-0028 OC-725C Oocyte amplifier

64-0030 Optional differential headstage for OC-725C

64-0034 TEV-700 Complete oocyte clamp workstation

Order # Product

89-5035 LIH 8+8

HEKA iTEV 90The iTEV 90 is a two-electrode voltage-clamp (TEVC) amplifier based on the technology of the EPC 10 USB amplifier. It features an integrated acquisition board (LIH 8+8) and is controlled entirely using PATCHMASTER software.

An additional third headstage can be connected for extracellular field compensation or additional intracellular current injection to increase the clamp speed of the amplifier. The third headstage is available as an option.

Acquisition Hardware & Software

Order # Product

89-5005 iTEV90 Multi-electrode amplifier


HEKA FITMASTERHEKA FITMASTER provides extensive offline-analysis of data collected either with CHARTMASTER or PATCHMASTER.

• Import data recorded with PATCHMASTER or CHARTMASTER

• Import data in ASCII-format

• Curve fitting via parameter optimization: exponential, polynominal, Hodgin-Huxlet, Gaussian and more

• Action potential analysis

Software updates are free of charge.

We offer a free webinar that supports beginners getting started with PATCHMASTER software. Please visit our website for details.

Recording

HEKA PATCHMASTER and CHARTMASTERPATCHMASTER provides amplifier control and data acquisition and analysis capabilities. In addition, it allows the user to automate their entire experiment and features built-in functionality for many other extensions (LockIn, imaging, spectroscopy, photometry). This makes PATCHMASTER the most versatile patch clamp software available.

CHARTMASTER is identical to PATCHMASTER with the exception that it does not have amplifier control capability.

Both software packages feature:

• Extended real time analysis

• Batch programming

• Comfortable data management

• Can be controlled from other software

• Solution management

• Export of data to e.g. Matlab, Igor, ASCII and others

• Runs on Windows and Mac platforms

• And many more features

Software updates are free of charge.

Also, extensions such as SmartLux, the imaging recording software, are available.

Order # Product

89-5040 PATCHMASTER

89-5041 PATCHMASTER Professional, GLP standard

89-5048 CHARTMASTER

89-5050 SmartLux Imaging Add-on

Order # Product

89-5046 FITMASTER

89-5047 FITMASTER Professional, GLP standard

Patch C

lamp, O


aging


Image courtesy of Alison North Rockefeller University.

The image at right illustrates how the components assemble to form a system.

The coverslip is sealed to the chamber and the sample is placed on the coverslip. If using slice preparations, the slice is held in place using a slice anchor (or hold-down) specifically designed for the chamber in use. Those hold-downs are press-fit into the respective chamber and are not weight dependent.

The chamber is placed into a platform. Warner Instruments has two platform styles available, with the magnetic design being simpler to use and much more convenient. The platform is then placed in a stage adapter which allows all Warner products to be placed onto a microscope stage.

Warner Instruments is the industry standard when it comes to imaging and recording chambers. We offer a wide variety of specialty chambers accommodating many applications. We also provide stage adapters to match almost every microscope stage.

Several components are needed for the proper use of an imaging and recording chamber:

• Chamber and coverslip (included)

• Platform

• Stage adapter

RC-22 chamber with slice anchor and patch electrode

MagneticSide Clamp

Chamber and platform with Olympus inverted stage adapter

Recording & Imaging Chambers


We offer many chambers that provide special properties and are optimized for specific applications. The following table provides an overview.

Ch

ambe

r O

rder

#

open

cl

osed

sm

all

med

ium

la

rge

patc

h tis

sue

oo

cyte

ep

ithel

ial

spec

ial

mic

ro-

35 m

m

50 m

m

field

up

right

pl

atfo

rms

ba

th

bath

vo

lum

e vo

lum

e vo

lum

e st

udie

s or

slic

e st

udie

s or

uss

ing

desi

gn

incu

batio

n cu

lture

cu

lture

st

imul

atio

n m

icro

scop

y

(< 1

00 µ

l)

(> 3

00 µ

l)

stud

ies

st

udie

s

cham

ber

dish

di

sh

sy

stem

Serie

s 20

RC

-20

64-0

222

•

•

P-

5, P

M-5

Ch

ambe

rs

RC-2

0H

64-0

223

•

•

P-

5, P

M-5

RC-2

1BR

64-0

225

•

•

• P-

2, P

M-2

RC-2

1BRF

S 64

-022

6

•

•

• •

P-2,

PM

-2

RC

-21B

DW

64

-036

2 •

•

•

P-2,

PM

-2

RC

-21B

RW

64-0

227

•

•

• P-

2, P

M-2

RC-2

2 64

-022

8 •

•

•

P-1,

PM

-1

RC

-22C

64

-022

9 •

•

•

P-1,

PM

-1

RC

-24

64-0

230

•

•

•

P-

1, P

M-1

RC-2

4E

64-0

231

•

•

•

P-

1, P

M-1

RC-2

4N

64-0

381

•

•

•

P-

1, P

M-1

RC-2

5 64

-023

2 •

•

•

•

P-3,

PM

-3

RC

-25F

64

-023

3 •

•

•

•

P-4,

PM

-4

RC

-26

64-0

234

•

•

•

•

• P-

1, P

M-1

RC-2

6G

64-0

235

•

•

•

•

• P-

1, P

M-1

RC-2

6GLP

64

-023

6 •

•

• •

•

P-1,

PM

-1

RC

-26G

S 64

-153

4 •

•

• •

•

•

PM-8

RC-2

6Z

64-0

237

•

•

•

• P-

1, P

M-1

RC-2

7 64

-023

8 •

•

•

P-6,

PM

-6

RC

-27L

64

-024

1 •

•

•

•

P-6D

, PM

-6D

RC

-27N

64

-023

9 •

•

•

•

P-6,

PM

-6

RC

-27N

E 64

-024

0R2

•

•

•

•

• P-

6, P

M-6

RC-2

9 64

-024

4 •

•

•

•

•

P-6D

, PM

-6D

Serie

s 30

RC

-30H

V 64

-023

1

• •

• •

•

•

Ch

ambe

rs

RC-3

0WA

64

-032

1

• •

• •

•

•

PFC-

1 64

-186

0

• •

• •

•

Ultr

a Q

uiet

JG

-23W

/HP

64-1

486

•

•

•

P-

1, P

M-1

Im

agin

g JG

-23W

/LP

64-1

488

•

•

•

P-

1, P

M-1

Ch

ambe

rs

RC-2

7D

64-1

548

•

•

•

•

• PM

-7

RC

-27L

D

64-1

532

•

•

•

•

• PM

-7D

YC-1

64

-167

8 •

•

•

Patch C

lamp, O


aging


Patch C

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aging


We offer many chambers that provide special properties and are optimized for specific applications. The following table provides an overview.

Ch

ambe

r O

rder

#

open

cl

osed

sm

all

med

ium

la

rge

patc

h tis

sue

oo

cyte

ep

ithel

ial

spec

ial

mic

ro-

35 m

m

50 m

m

field

up

right

pl

atfo

rms

ba

th

bath

vo

lum

e vo

lum

e vo

lum

e st

udie

s or

slic

e st

udie

s or

uss

ing

desi

gn

incu

batio

n cu

lture

cu

lture

st

imul

atio

n m

icro

scop

y

(< 1

00 µ

l)

(> 3

00 µ

l)

stud

ies

st

udie

s

cham

ber

dish

di

sh

syst

em

Ooc

yte

RC-1

Z 64

-031

8 •

•

•

•

Ch

ambe

rs

RC-3

Z 64

-031

9 •

•

•

•

•

Quic

k

RC-4

0LP

64-0

367

•

• •

• •

•

• Q

E-1

Co

vers

lip

RC-4

0HP

64-0

370

•

• •

• •

•

• Q

E-1

Ex

chan

ge

RC-4

1LP

64-0

368

•

•

•

•

•

• Q

E-1

Ch

ambe

rs

RC-4

2LP

64-0

369

•

•

•

•

•

Q

E-1

RC-4

3C

64-0

371

•

•

•

•

•

• Q

E-1

RC-4

7FSL

P 6

4-03

74

•

•

•

•

•

•

• Q

E-1

RC-4

8LP

64-0

387

•

•

•

•

•

Q

E-1

Cultu

re D

ish

RC-3

3DM

64

-148

1 •

•

•

In

sert

s RC

-33D

L 64

-148

2 •

•

•

35

mm

dish

es R

C-33

SN

64-1

483

•

•

•

RC

-33S

L

•

•

•

RC

-37W

64

-034

7 •

•

•

RC-3

7WS

64-0

365

•

•

•

RC

-37F

64

-034

8 •

•

•

RC-3

7FC

64-0

364

•

•

•

•

RC

-37F

S 64

-036

6 •

•

•

•

Cultu

re D

ish

QE-

1 64

-037

5 •

•

•

Pl

atfo

rms

QE-

2 64

-154

2 •

•

•

Mic

ro-

DH

-35

64-0

110

•

• •

incu

batio

n D

H-3

5iL

64-0

349

•

• •

D

H-4

0iL

64-0

388

•

• •


This table lists applications with suitable chambers.

Finding Chambers by Application

Closed Bath Chambers

CV-30 CytoViva™ Environmental Chamber

RC-20 and RC-20H Small Volume Closed Bath Imaging Chambers

RC-21BR Large Closed Diamond Bath

RC-21BRFS Slotted Bath with Field Stimulation

RC-30HV, and RC-30WA Confocal Imaging Chambers

RC-37 Series Perfusion Inserts for 35 mm Dishes

RC-43C Closed Bath Chamber

Field Stimulation Chambers


RC-27NE Narrow Bath Chamber with Field Stimulation

RC-37 Series Perfusion Inserts for 35 mm Dishes

RC-47FSLP Slotted Bath Chambers

Large Volume Chambers (> 300 µl)

RC-21BR Large Closed Diamond Bath

RC-27 Large Rectangular Open Bath Chamber

RC-27D and RC-27LD Ultra-quiet Imaging Chambers for Slice Studies

RC-27N Narrow Rectangular Baths


RC-27L Large Bath Chamber with Slice Supports

RC-29 Very Large Rectangular Open Bath for Slice Studies


RC-40HP High Profile Open Bath Chamber

RC-40LP, RC-41LP, RC-42LP, and RC-48LP Low Profile Open Bath Chambers

Microincubation Chambers

PDMI-2 Open Perfusion Micro-Incubator

DH-35 Culture Dish Heater

DH-35i Culture Dish Incubator

DH-40i Micro-Incubation System

QE-1 Quick Exchange Platform

QE-2 Quick Exchange Heated Platform with Perfusion






Oocyte Chambers

RC-1Z Oocyte Recording Chamber


RC-26Z Open Diamond Bath for Oocyte Studies



Patch Clamp Chambers

RC-24 and RC-24E Fast Exchange Diamond Bath Chambers

RC-24N Fast Exchange Open Diamond Bath

RC-25 and RC-25F Open Diamond Bath Chambers for Round Coverslips

RC-26 and RC-26G Open Diamond Bath

RC-26GLP Low Profile Open Diamond Bath

RC-26GS Silicone Imaging Chamber




Suitable for Upright Microscopy



RC-21BDW Open Diamond Bath and RC-21BR Large Closed Diamond Bath


RC-21BRW Open Round Bath

RC-25 and RC-25F Open Diamond Bath Chambers for Round Coverslips

RC-26 and RC-26G Open Diamond Bath

RC-26GLP Low Profile Open Diamond Bath

RC-26GS Silicone Imaging Chamber

RC-26Z Open Diamond Bath for Oocyte Studies

RC-27D and RC-27LD Ultra-quiet Imaging Chambers for Slice Studies

RC-27L Large Bath Chamber with Slice Supports

RC-27N Narrow Rectangular Baths








Open Bath Chambers

Patch C

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aging


Examples are provided that illustrate the basic composition of the chambers.

The majority of the chambers consist of these sections:

• Imaging/recording zone• Perfusion input• Suction tube• Agar bridge well

Most of the chambers incorporate diamond-shaped fluidics reservoir yielding laminar flow throughout the bath. Low profile chambers allow excellent access without interfering with optical measurements.

RC-24E

Perfusion Input

Suction Tube

Agar Bridge Well

RC-26, RC-26G and RC-26GLP The RC-26, RC-26G and RC-26GLP provide a large volume in an open bath diamond shaped chamber that accommodates large specimens such as slice preparations.

• Optimized for patch clamp studies

• Can be used for tissue slice samples or cell coverslips

• Large imaging area

• Supports upright and inverted microscopes

• Slice anchor available

RC-26G

Suction Tube Clamp

Suction Tube Clamp

Agar Bridge Well

RC-25 and RC-25F The RC-25 and RC-25F chamber.

• Designed for physiological measurement of cell cultured coverslips

• Diamond-shaped for laminar solution flow

• Applications such as patch clamp, intracellular/extracellular recordings and imaging

• Small bath volume facilitates fast solution exchange

• Uses 12mm (RC-25) or 15mm (RC-25F) round coverslips

RC-20 and RC-20H The RC-20 and RC-20H are closed-bath chambers. A secondary perfusion input can be used to inject substances or remove air bubbles. The chamber also enables a short working distance.

• Closed-bath design

• Very small volume

• Uses 15 mm round coverslip

• Gas tight design at chamber interface

RC-20H

RC-25F

Perfusion Input

Perfusion Input

Top CoverslipRetainer

SecondaryPerfusion

Input

Suction Tube

Suction TubeSuction

ReservoirAgar Bridge Well

Chambers

Order # Product

64-0234 RC-26, 170 µL volume

64-0235 RC-26G, 234 µL volume

Order # Product

64-0232 RC-25, 90 µL volume

64-0233 RC-25F, 133 µL volume

Order # Product

64-0222 RC-20, 35 µL volume

64-0223 RC-20H, 70 µL volume

Closed Bath Chambers


RC-21BRFS

PerfusionInput

SecondaryPerfusion Input

RC-40LP

Image courtesy of John Geible using the JG-23 chamber.

Suction Tube

Chambers

RC-40 FamilyThe RC-40 chamber family was designed for research requiring the fast exchange of round cover slips. The quick release magnetic imaging chambers are compatible with the QE-1 quick exchange platform, DH-35iL and DH-40iL culture dish incubators.

• Dual o-ring seal system enabling quick coverslip exchange, ideal for rapid screening assays

• Closed bath design promoting smooth continuous solution exchange as well as stable imaging focus

• Anodized aluminum base guarantees effective heat transfer

RC-21BRFS The RC-21BRFS is a modified version of the RC-21.

• Closed bath design

• Small bath volume with slotted bath

• Requires 25mm round cover glasses for top and bottom of the chamber

• Volume of 263 µl

• Gas tight design at chamber interface

Warner Instruments provides field stimulation chambers. All field stimulation chambers are equipped with platinum electrodes that are attached on the sides of the bath. The cables are terminated with 1 mm pins.

• Designed for field stimulation studies involving cardiac myocytes

• Available in open and closed bath chamber design

• Ideal for fluorescence, calcium and time lapse imaging studies

Order # Product

64-1943 QR-40LP for 25 mm coverslip, low profile




64-1947 QR-40HP for 25 mm coverslip, high profile

64-1948 QR-43C for 25 mm coverslip, closed bath

64-1949 QR-41SLP for 25 mm coverslip, slotted bath

64-1950 QR-46NSLP for 25 mm coverslip, narrow slotted bath

64-1951 QR-47FSLP for 25 mm coverslip for field stimulation

Order # Product

64-0226 RC-21BRFS for field stimulation

Quick Release Chambers Field Stimulation Chambers

Perfusion Inserts for 35 mm Dishes

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Suction Tube

RC-27NE2

PerfusionInput

SecondaryPerfusionInput

RC-49MFSH

RC-27NE2 The RC-27NE2 is a modified version of the RC-27 with a narrower bath and smaller volume.

• Narrow open bath design

• Rectangular shape

• Accommodates tissue and brain slice specimens

• For applications such as patch clamp and physiological measurements on cultured cells

RC-37WS and RC-37FSThese chambers make perfusion of cell culture dishes a simple matter.

• Glass bottomed 35mm cell culture dishes

• Narrow bath design

• Use DH-35iL platform providing microincubation

Microincubation System DH-35iL and 40iLWarner Instruments also provides microincubation platforms such as DH-40iL and DH-35iL. The DH-40iL is designed to support glass-bottomed 35 mm cell culture dishes and the RC-40 family open bath chambers. The DH-35iL supports glass-bottomed 35 mm cell culture dishes and Series 30 open bath chambers.

• Permitting imaging, temperature and gas environment control

• Compatibility with Corning, Falcon, MatTek, Nunc, Willco Wells and WPI Dishes (DH-40iL only in open configuration)

• Unique dish clamps providing easy cell access

RC-49MFSH The RC-49MFSH is designed for the quick exchange platform QE-1.

• O-Ring seal permits quick exchange of coverslips

• Low profile design allows low entry angle electrodes

• Platinum field stimulation electrodes

• Uses popular 18 mm round coverslips

• Embedded heating elements

Order # Product

64-0240R2 RC-27NE2 narrow bath for field stimulation

Order # Product

64-0365 RC-37WS chamber insert for Willco 35mm cell culture dishes for field stimulation

64-0366 RC-37FS chamber insert for Falcon 35mm cell culture dishes for field stimulation

Order # Product

64-0349 DH-35iL culture dish incubation system supporting 35 mm quick exchange chambers

64-0388 DH-40iL culture dish incubation system supporting RC-40 family and 35 mm quick exchange chambers

Order # Product

64-1725 RC-49MFSH magnetic imaging/recording chamber with removable electrodes for field stimulation and heating elements


Model QE-1 with RC-42LP low profile 15 mm coverslip chamber

Suction Outlet

Chambers

Order # Product

64-0375 Quick change platform, heated base, for RC-40 and QR-40 series chambers

64-1542 Quick change chamber, heated base, for 50 mm culture dishes

Each chamber requires a specific platform. Most of the chambers are supported by three families of platforms, the P, PH and PM. The P-family comes with brakes that are held in place with screws. These brakes press down to secure the chamber. The mechanical system of the PH-family is identical but additionally these platforms can be heated. The third family, PM, are magnetic, so no tools are required to remove the chambers and replacement of the chambers is quick and convenient.

Stage Adapters The platforms have identical outer dimensions. A stage adapter is needed to mount a platform onto a microscope stage. The cutout of the stage determines the corresponding adapter. Warner Instruments adapters support stage cutouts from all manufacturers, including:

• Burleigh Gibraltar

• Leica

• Nikon Marzhauser

• Olympus

• Prior & Ludl

• Scientifica

• Zeiss

• Multiwell stages

Please visit our website to find a suitable adapter.

QE-1 This platform is designed to serve the RC-40 family as well as 35mm glass bottom dishes.

• Supports inverted and upright microscopes

• Resistive heating elements for temperature control

• Provides microincubation in combination with DH-35iL or DH-40iL

• Removable perfusion and suction holders

• Adapter available for Willco Wells, Corning, and Falcon dishes

Platforms

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Silicone Grease An artist’s acrylic brush is an effective tool for applying silicone lubricant to a glass coverslip and polycarbonate chamber. By “painting” the grease onto the bottom surface of a polycarbonate chamber, it is easy to evenly spread lubricant and create a water-tight seal.

The silicone grease kit includes:

• Tube of Dow Corning® 111 Valve Lubricant & Sealant

• Two acrylic paint brushes (sized #2 and #4)

• Several pallets

Slice AnchorsThe slice anchors are designed for an easy-press fit into the chamber’s bath area. This allows control of the cord line pressure applied to the tissue slice. Most anchors are made of type 316 stainless steel with Lycra© threads and finished with plastic coating. Some anchors are available in plastic. Please visit our website to find the right anchor for Series 30 or Series 40 chambers.

Order # Product

64-0378 Silicone grease kit, includes brushes and pallets

64-0275 Stopcock grease

Coverslips

Warner Instruments provides a number of borosilicate glass coverslips in different thicknesses and sizes for microscopy and imaging. Please visit our website to find the right coverslips for your application.

Chamber Accessories


Single Channel TC-324C Resistive Temperature Controller

Battery-Powered Dual Channel TC-144 Temperature Control

Researchers have long understood the importance of temperature regulation in the study of cellular function:

• Sources of heat flux

• Space and time factors

• Outgassing of perfusate solutions

• How temperature is sensed

• In-line solution heating

• Chamber platform heating

• Solution reservoir heating

• Microscope objective heating

• Heated enclosures

Transmission of thermal energy can happen through the microscope objective, the perfusion solution, the chamber platform and any other physical elements of the working environment. We provide temperature control of three parts:

• Platform

• Solution

• Microscope objective

Heated platforms will transmit the heat to the chambers on the sides only, therefore the temperature will have a gradient from the sides to the middle of the chamber.

Heating the solution can work well if the flow rate is sufficient Objectives are a huge heat sink, especially in case of immersion.

Dual Channel CL-200A Bipolar Temperature Controller

The temperature is adjustable up to 65° C. The instruments are optimized for low noise setups.

LCS-1 Liquid Cooling System

Temperature Control Systems

Background

Temperature Controllers

Order # Product Properties Suggested Applications

64-1545 TC-124A Battery-powered single channel temperature controller • Microscope objective heater

• Syringe heater

• Stage insert heater64-1655 TC-144 Battery-powered dual channel temperature controller

64-2400 TC-324C Single channel temperature controller • Syringe heater

• Stage insert heater

• In-line solution heater, culture dish incubation system64-2401 TC-344C Dual temperature controller

64-0352 CL-100 Bipolar single channel temperature controller • In-line solution heater/cooler

• Stage insert heater/cooler64-1723 CL-200A Bipolar dual channel temperature controller

These are the products and applications that we offer:

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Heating & Cooling PlatformsThese platforms are designed to work with a bipolar temperature controller and will also work with the inline-solution heater/cooler SC-20, described in the following section.

The bipolar temperature controller also requires the Liquid Cooling system, order number 64-1922, in order to function properly.

Model QE-1HC with 35 mm glass bottom dish

TB-3 CS temperature control for chamber slides

Model TB3 CCD fits into Prior NZ100 and NZ200

Magnetic clamps

Magnetic stainlesssteel

35 mm Dish Clamps

Water Jack Ports

Order # Product Properties

64-1659 QE-1HC quick exchange platform

Easy access for imaging64-1632 TB-3 CS Thermal insert

supporting 1 x 3” slides

64-1636 TB-3 CCD Thermal insert supporting RC-40 quick exchange chamber

64-1646 TB-4 CS Thermal insert supporting 1 x 3” slides

Improved heat conductance

Temperature Control Accessories


Objective HeatersObjective heaters are extremely important for immersion optics.

• Reduced thermal gradient between objective and sample

• Have no direct contact between warmer and objective

• Fit microscope objectives from most manufactures

In-Line Solution Heaters/CoolersThe simplest and most direct approach for the application of heat to a sample is to preheat the perfusion solution immediately prior to its delivery to the chamber. Warner Instruments provides a wide variety of solution heaters including single channel slow-flow and fast-flow models.

If multiple solutions are required, the multi-line solution heater is the best option to be able to quickly change your solution and heat it properly. Our in-line solution heater can heat up to 50° C.

In-Line Solution Heater HoldersWe provide the following in-line-solution heater holders:

SC-20

SHM-6

SF-28

SH27-B

SHM-828

Temperature Control Systems

Order # Product Max Flow Rate

Inputs Outputs Heating/Cooling

64-0103 SF-28 In-Line solution heater

2 ml/min 1 1 H

64-0102 SH-27B In-Line solution heater

5 ml/min 1 1 H

64-0104 SHM-6 6-line solution heater

5 ml/min 6 1 H


5 ml/min 8 1 H


5 ml/min 8 8 H

64-0353 SC-20 In-Line heater/cooler

5 ml/min 2 1 H/C

Order # Product

64-1555 SSH-1, holder for SH-27B and SF-28

64-1556 SSH2, holder for SC-20

64-1557 Holder for SHM-6, SHM-8, SHM-628

64-1558 Holder for FR-50 and FR-55S flow valves


64-1664 OWS-1 Objective Warmer System, for 23-30 mm objectives, includes TC-124A temperature controller

64-1676 OWS-2 Objective Warmer System, for 30-35 mm objectives, includes TC-124A controller

64-1665 OW-1 Objective warmer, for 23-30 mm objectives, requires TC-124A or TC-144 controller

64-1674 OW-2 Objective warmer, for 30-35 mm objectives, requires TC-124A or TC-144 controller

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Perfusion is required to keep the specimen alive and can also be used for heating or cooling. Most of the perfusion systems available consist of a set of syringes filled with solution and a valve controller that opens and closes the valves of each syringe. Syringe tubing consolidates in a manifold which is connected to the chamber.

To avoid overflow of the solution, suction must be applied. This is usually done with a vacuum system.

Alternatively, a peristaltic pump can be used for delivering solution as well as for suction.

Perfusion Systems VC-8 and VC-6Warner Instruments offers a six channel (VC-6) and an eight channel (VC-8) valve controller. Both can be controlled via TTL which requires one connection per channel. Additionally each valve has its own toggle switch to manually switch it on or off.

• Spill sensor input which detects an overflow on the microscope and shuts off the perfusion

• Valve-control with analog signals: using only one analog output channel of your acquisition system can open one of the valves at the time using different voltage levels per valve

• Automatic valve detection

• 2 additional perfusion channels compared to VC-6

• Event marker for acquisition system showing manual valve status change

Pinch Valve PTFE Valve

Perfusion Control

Order # Product Channels Version Valves Specialty

64-0135 VC-6 6 complete pinch -

64-0138 VC-6 6 complete PTFE -

64-0174 VC-6 6 complete mini -

64-0174LT VC-6 6 basic mini large tubing

64-0129 VC-6 6 basic pinch -

64-3132 VC-6 6 basic PTFE -

64-0171 VC-6 6 basic mini -

64-0185 VC-8 8 complete pinch -

64-0187 VC-8 8 complete PTFE -

64-0186 VC-8 8 complete mini -

64-0186LT VC-8 8 complete mini large tubing

Background

Both controllers support three different valves: Pinch, PTFE and Miniature Valves.

Pinch Valves are the simplest valves to maintain as the solution never gets in touch with the valve and tubing can easily be changed. Valves are dual acting (3-way) with both normally open and closed sides. Y connectors at the valve input permit solution flow to waste with the valves off.

PTFE Valves are available for applications where resistance to chemicals is a concern. The valves are 2-way, either on or off.

Miniature Valves are designed for slow flow perfusion systems where smaller diameter tubing is used. The valves mount directly to a compact Delrin manifold. The 3-way valves allow for solutions to flow to waste if desired. These are ideally suited for use with the SF-77B Fast-Step Perfusion Stepper Systems.

The perfusion systems are available in a Basic version that comprises of the following:

• Valve controller

• Valves

• Valve bracket, including an 8 ft long cable to be connected to valve controlled

• MP series manifold

The complete version also includes:

• 60cc (10cc for mini-valves) reservoirs (syringes)

• Reservoir holder

• Ring stand

• Stopcocks for each reservoir

• Tubing connector

VC-8 Perfusion System

Perfusion Systems


Fast Step Perfusion System SF-77BIf very fast change of the solution is required, Warner Instruments has the answer: the Fast-Step perfusion system SF-77B.

The SF-77B controls continuous solution flow through adjacent stimulus delivery tubes and a stepper mechanism that selects which tube is directed at the preparation. Complete solution changes are typically achieved within 20 ms for a large 700 mm step and times are significantly shortened as the step size is decreased.

The stepper mechanism can be controlled manually via the front panel or externally directed from a data acquisition program. It requires either an analog signal coding the required position or uses 3 TTL inputs.

More variations are available, please visit our website.

Peristaltic Pump PPS2If only one incoming and suction solution is required, the PPS2 is the perfect device.

• 2 channels (1 in/1 out or 2 in or 2 out)

• Control at instrument via touchscreen

• SW control (requires USB connection to Windows-PC)

• Control using TTL and analog voltage provided by e.g. acquisition board

• Flow rate 0.1 up to 30 ml/min in 0.1ml/min steps

• Link both channels by percentage, e.g. suction is 105% of incoming solution

• Bubble detector for suction control

Dedicated Workstation Vacuum DWVThe DWV provides suction to prevent solution overflow in the chambers using a perfusion system.

• Completely self-contained liquid waste system

• High quality low-noise vacuum pump, 40 dB(A) maximum

• Dual flask design for improved vacuum stability

If the incoming solution does not need to get changed, the PPS2 replaces a perfusion system and a vacuum system for suction.

Perfusion Systems

Order # Product Additional Information

64-0114 SF-77B 110V

64-0115 SF-77B 110V, Theta tubing

64-1515 SF-77B 200-240V

64-1516 SF-77B 200-240V, Theta tubing

64-0177 VC-77SP: SF-77B, VC-6, right-handed micromanipulator, magnetic base

115 V

64-1519 200-240 V, Theta tubing

64-0177L VC-77SP: SF-77B, VC-6, left-handed micromanipulator

115 V

64-0177LE 220 V, Theta tubing

64-1590 VC-77SP: SF-77B, VC-8, right-handed micromanipulator, magnetic base

115 V

64-1590E 220 V

Order # Product

89-0688 Peristaltic Pump, 2 channels

89-0692 Peristaltic Pump, 5 channels

Order # Product

64-1940 Dedicated Workstation Vacuum

Manifolds

Solutions A1-A6 Solutions B1-B6

Approximately 5 secondsfor new solution flow when switching manifold input

Approximately 30 secondsfor new solution flow when adding new reservoirs

Stimulus effect in 20 milliseconds for 700 mm step size

PE-10 Tubings

PE-50 Tubings

Solutions C1-C6

Glass Tubes

Cell Attached Electrode

A B C

Waste Systems

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Miscellaneous Perfusion AccessoriesWarner Instruments also provides these accessories:

Please visit our website for a full selection of our accessories.

• Syringes, syringe holder

• Tubing

• Valve control parts

• Gas humidification system

• Gas bubbler manifolds

• Perfusion pressure kits

• Manifold holder

Hybrid StageWe offer a hybrid stage for patch clamp studies and other experiments. The XY-translator can either be used manually or motorized. The platform is available for inverted or upright microscopes with US or metric threads. It supports the most common microscopes. This part list is not comprehensive, please visit our website for a full list of options.

Stage

Order # Product

64-0220 FR-50, flow valve

64-0221 FR-50S, flow valve with on/off-switch

Order # Product

64-2365 Stage with XY translator, fits Nikon Eclipse T inverted, US thread

64-2366 Stage with XY translator, fits Leica DMI8 inverted, US thread

64-2367 Stage with XY translator, fits Olympus IX-73 inverted, US thread

64-2368 Stage with XY translator, fits Zeiss Axiovert inverted, US thread

64-2384 Stainless steel insert for stage

64-2385 Stainless steel plates, 3 pieces

69-5000 Wheel input device for hybrid stage, requires 695001

69-5001 Controller for hybrid stage

Perfusion Accessories

ManifoldsManifold inputs converge to the common output with a minimum of dead space. They are designed for use with PE-160, PE-50, and PE-10 polyethylene tubing, but they can also be used with other tubings with similar dimensions.

Vacuum and Flow RegulatorThe FR-50/FR-55S is a convenient tool to adjust both solution flow rates and vacuum pressure in a variety of applications. The solution flow is adjustable from zero to a maximum of 10 mL/min (measured with a solution head of 30 cm). The units have calibrated adjustment rings to permit returning to a predetermined setting.


MicromanipulatorsMicromanipulators are required to position the micropipette for recording or injection relative the specimen. Warner Instruments provides micromanipulators optimized for injection as well as high quality models for patch clamping.

• Mechanical resolution better than 10 nm

• Zero-drift technology

• A small footprint on the stage, due to their compact design

• Travel range X/Y/Z 20 x 20 x 20 mm

Please visit our website for additional options.

HEKA PIP 6Pullers are required to produce micropipettes that meet specific tip diameter and shape requirements for experiments.

The PIP 6 vertical pipette puller has been doing the job for a decade.

• Two programmable temperatures

• Temperature ranging 300°-1900° C

• Supports capillary glass up to 150 mm

• Pulls glass ranging 1-3 mm

Programmable Pipette Puller PMP-102The PMP-102 is a horizontal puller that pulls two identical pipettes.

• 25 programmable sequences

• Programmable multi-pulling steps

• Pneumatic adjustable pulling force

• 22 pre-programmed sequences for commonly used pipette tips

Micromanipulator Pipette Puller

Order # Product

69-3437 Complete µM system

69-3432 (µMp-3) µM manipulator, three axis, simple bolt-on mounting base

69-3433 (µMp-4) µM manipulator, four axis, simple bolt-on mounting base

69-3426 (µM-TSC) µM touch screen control unit

69-3427 (µM-RW3) µM rotary wheel remote interface (3 wheel)

69-3428 (µM-RW4) µM rotary wheel remote interface (4 wheel)

69-3429 (µM-HUB) µM 1 to 4 hub with extra battery

69-3434 µM Add-on flip/side rotate module

69-3435 (µM-FVT) uM dove-tail headstage adapter

69-3436 (µM-MPR) uM multipurpose adapter for 5-10mm diameter electrode holders/rods

Order # Product

89-5052 PIP 6 vertical puller

89-5053 Heating filaments, pack of 10

Order # Product

69-0151 PMP-102 programmable puller

69-0172 Replacement heat coil

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Capillary GlassWarner Instruments provides a wide range of capillary glass.

• Length 75–150mm

• With and without filament

• Clark borosilicate, aluminosilicate and premium borosilicate glass

• Thin wall and standard

• Outer diameter 1–300mm

Please visit our website to see the full selection.

Microinjection SystemThe use of microcapillary pipette based techniques for intracellular/extracellular microinjection and perfusion has become a popular procedure in numerous areas of experimental biology research. Warner Instruments has a complete product line fulfilling the needs for these applications.

The top-model PLI-100A as well as the PLI-90A and the base model PLI-10 feature:

• Injection pressure of 0.2-60 PSI (413 kPa)

• Injection Time of 0.01 to 99.99 seconds

• Trigger mode: front panel, footswitch or TTL (Gate In)

• Pressure monitor output: BNC, rear panel

• Injection time accuracy of ±0.01% (Crystal Time Base)

• Injection count display: 0-9999 injections

• Clearing pressure (PLI-100A and PLI-90A only): Input gas pressure, unregulated

• Balance pressure (PLI-100A and PLI-90A only): Regulated, multiturn control, other ranges available on request of 0.1 to 9.9 PSI (68.9 kPa)

• Fill Vacuum (PLI-100A only): Internally produced 0 to -12 PSI (0 to-82 kPa), regulated

• Holding Vacuum(PLI-100A only): Internally produced 0 to -0.1 PSI (0 to -0.75 kPa), regulated

We also offer a microinjector system consisting of:

• PLI-100A microinjector

• Magnetic base, manual controlled micromanipulator

• Electrode holder

• Light VLux with single arm gooseneck

• Binocular Zoom Stereo Microscope on ball bearing stand

Please visit our website for details.

Microinjector System

MicroinjectionCapillary Glass


64-1735 PLI-100A 5 pressures

64-1738 PLI-90A 3 pressures

64-1707 PLI-10 1 pressure

64-2825 Microinjector System –

64-1737 PLI-FS Foot switch –


PatchServerThe PatchServer is world-wide the only automatic patch-clamp system that is able to establish single-channel and whole-cell recording configurations using tools and techniques from the manual patch approach. The PatchServer is an add-on tool for manual patch-clamp setups. It utilizes standard glass electrodes and employs step-by-step the manual patch-clamp procedure of a human experimenter.

• Makes manual patching simpler and more efficient

• Automatically establishes all recording configurations

• Utilizes manual patch clamp technology (glass pipettes)

• Highest data quality with increased data production rate

Patch Clamp

Order # Product

89-0679 PatchServer-1-Basic

89-0495 PatchServer-1-Complete-HEKA

89-0497 PatchServer-1-Standard-HEKA

89-0498 PatchServer-4-Basic

89-0500 PatchServer-4-Complete-HEKA

89-0502 PatchServer-4-Standard-HEKA

Automated Pipette-Based Patch-Clamp

Scientific Meetings

Come see us at the following annual meetings...

Biophysical Society

Experimental Biology

Society for Neuroscience

Electrophysiology & Live-Cell Imaging

divisions of Harvard Bioscience, Inc.

Harvard Apparatus Canada

6010 Vanden Abeele Saint-Laurent, Quebec H4S 1R9, Canada

phone +1.514.335.0792

toll free +1.800.361.1905 (CAN Only)

fax +1.514.335.3482

e-mail [email protected]

web www.harvardapparatus.ca

HEKA

HEKA Elektronik Dr. Schulze GmbH Wiesenstraße 71 67466 Lambrecht/Pfalz, Germany

phone +49 6325 9553 0

fax +49 6325 9553 50


web www.heka.com

Warner Instruments

1125 Dixwell Avenue, Hamden, CT 06514, USA

phone +1.203.776.0664

toll free +1.800.597.0580 (USA Only)

fax +1.508.429.5732


web www.warneronline.com

Multi Channel Systems

Aspenhaustrasse 21 72770 Reutlingen, BW Germany

phone +49 7121 90925 25

fax +49 7121 90925 11


web www.multichannelsystems.com

Harvard Apparatus

84 October Hill Road Holliston, MA 01746, USA

phone +1.508.893.8999

toll free +1.800.272.2775 (USA Only)

fax +1.508.429.5732


web www.harvardapparatus.com

Harvard Apparatus China

Room 1902E, 19F, Building B Zhong Shan Plaza 1065 West Zhong Shan Road Changning DistrictShanghai, China

phone +86.21.2230.5128


Documents

Electrophysiology & Live-Cell Imaging