February 2017 · 2017. 2. 27. · Cigarette Tobacco Filler was prepared from air-cured very low nicotine tobacco that was processed using ... tobacco specific nitrosamines (TSNAs),

301-975-2200 phone 301-948-3730 fax http://www.nist.gov/srm [email protected]

February 2017

NIST SRM 2082 Pathlength Absorbance Standards for Microliter Volume Spectrophotometers

The determination of the concentrations of DNA, RNA, and proteins based on absorbance is one of the most frequently used measurements in biological laboratories. A new generation of spectrophotometers use short pathlengths and microliter volumes to conserve precious samples. SRM 2082 was designed to provide a rapid and convenient method to verify the pathlength in the UV-range using materials that have similar absorbance characteristics to nucleic acids and proteins. The selection criteria for the materials are that the spectral properties should be similar to nucleic acids and proteins and the solutions had to be stable. Uracil and tryptophan met these criteria. Uracil is one of the bases found in RNA and has an absorbance spectrum (peak at 260 nm) that is similar to those of RNA and DNA. The amino acid tryptophan is one of the twenty amino acids naturally occurring in proteins and is the amino acid mainly responsible for the characteristic absorbance of proteins (peak at 280 nm). A set of calibrated short pathlength cuvettes was used to determine the absorbances of the components. The effect of temperature and spectral bandwidth on the absorbance values was also measured. The stability was shown by repeated measurements over 2 years.

Technical Contact: Kenneth Cole Email: [email protected]

https://www-s.nist.gov/srmors/view_detail.cfm?srm=2082

Inside: New SRMs/RMs

SRM 2082 Pathlength Absorbance Standards for Microliter Volume Spectrophotometers

SRM 3060 Monoester Phthalates in Acetonitrile

SRM 3222 Cigarette Tobacco Filler SRM 3239 Isoflavones Calibration Solutions SRM 3600 Absolute Intensity Calibration Standard for Small-Angle X-ray Scattering

RM 8375 Microbial Genomic DNA Standards for Sequencing Performance Assessment

RM 8391 Human DNA for Whole-Genome Variant Assessment (Son of Eastern European Ashkenazim Jewish Ancestry)

RM 8392 Human DNA for Whole-Genome Variant Assessment (Trio of Eastern European Ashkenazim Jewish Ancestry)

RM 8393 Human DNA for Whole-Genome Variant Assessment (Son of Chinese Ancestry)

PITTCON Speakers Renewal SRMs Revisions NIST SRM Exhibits The identification of any commercial product or trade name does not imply endorsement or recommendation by the National Institute of Standards and Technology.

Editor: Regina R. Montgomery

http://www.nist.gov/srmmailto:[email protected]://www-s.nist.gov/srmors/view_detail.cfm?srm=2082

SRM Spotlight Page 2 February 2017


NIST SRM 3060 Monoester Phthalates in Acetonitrile

The Centers for Disease Control and Prevention (CDC) have been monitoring monoester phthalates in human urine samples as part of the National Health and Nutrition Examination Survey (NHANES) since NHANES 1999-2000. Several years of NHANES data had to be revised recently due to a purity issue with several monoester phthalates. After spending a considerable amount of time and money revising monoester phthalate data, in 2012, CDC approached NIST and asked if NIST would consider developing a solution SRM for monoester phthalates. To this end, NIST decided to prepare a solution of 13 monoester phthalates with relative concentrations similar to those observed in urine by NHANES. NIST developed SRM 3060 Monoester Phthalates in Acetonitrile. This solution is an assemblage of the most frequently detected monoester phthalates in urine and is intended to be used in the validation of existing methods or for use as a calibration mixture in routine analysis. Technical Contact: Jessica Reiner Email: [email protected]


NIST SRM 3222 Cigarette Tobacco Filler

The National Institute of Standards and Technology has collaborated with the Food and Drug Administration, Center for Tobacco Products (FDA CTP) to develop a Standard Reference Material (SRM) to support the analysis of tobacco products. SRM 3222 Cigarette Tobacco Filler was prepared from air-cured very low nicotine tobacco that was processed using normal procedures for the production of cigarette tobacco filler. The resulting material consists of dried and chopped leaves, referred to as “cut rag”, with 30 cuts per inch. This SRM is intended primarily for use in evaluating the accuracy of procedures for the determination of nicotine, tobacco specific nitrosamines (TSNAs), N-nitrosonornicotine (NNN), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), and moisture in tobacco. It is also intended for use in validating working or secondary reference materials. A unit of SRM 3222 consists of 20 jars, each containing approximately 10 g of cigarette tobacco filler.

Technical Contact: Lane Sander Email: [email protected]


http://www.nist.gov/srmmailto:[email protected]://www-s.nist.gov/srmors/view_detail.cfm?srm=3060https://www-s.nist.gov/srmors/view_detail.cfm?srm=3222



NIST SRM 3600 Absolute Intensity Calibration Standard for Small-Angle X-Ray Scattering

Standard Reference Material (SRM) 3600 Absolute Intensity Calibration Standard for Small-Angle X-Ray Scattering is intended for use in the scattering intensity calibration of small-angle X-ray scattering (SAXS) instruments. Absolute intensity calibration of SAXS data is a critical requirement for the quantitative determination by SAXS of volume fraction (or porosity) and surface area information for nanoscale and mesoscale structures within advanced technological materials. Frequently, it is such details of the microstructure that determine the key properties of the material, and hence its performance in an application. Direct measurement of the SAXS scattering probability of a given sample material requires absolute calibration of the weak scattered beam intensity relative to that of the incident beam. This requires a linear detector response to X-ray intensity over a very large dynamic range. In contrast, SRM 3600 provides a convenient intensity calibration through comparison of the normalized scattering intensities measured for the sample and the standard.

SRM 3600 is intended for use in materials microstructure characterization, as well as for quality assurance and establishing traceability when assigning values such as volume concentration or internal surface area to a broad range of heterogeneous materials of technological importance, including (but not limited to) precipitate formation in metals and alloys, sintered morphologies in functional ceramics, nanoparticle suspensions for biomedical applications or otherwise important to the environment and health, porous catalysts and sorbents, new polymer processing, and protein folding relevant to new drug development. A unit of SRM 3600 consists of one glassy carbon coupon of approximate dimensions 10 mm x 10 mm x 1 mm (thick), supplied in a plastic membrane box, together with a certified intensity calibration curve.

Technical Contact: Andrew Allen Email: [email protected]


http://www.nist.gov/srmmailto:[email protected]://www-s.nist.gov/srmors/view_detail.cfm?srm=3600



NIST RM 8375 Microbial Genomic DNA Standards for Sequencing Performance Assessment

NIST RM 8391 Human DNA for Whole-Genome Variant Assessment (Son of Eastern European Ashkenazim Jewish Ancestry)

NIST RM 8392 Human DNA for Whole-Genome Variant Assessment (Trio of Eastern European Ashkenazim Jewish Ancestry)

NIST RM 8393 Human DNA for Whole-Genome Variant Assessment (Son of Chinese Ancestry)

In September 2016, NIST released four new human and microbial whole genome reference materials characterized by NIST and the Genome in a Bottle Consortium (GIAB). GIAB is a public-private-academic consortium hosted by NIST and the Joint Initiative for Metrology in Biology (JIMB, http://jimb.stanford.edu), a collaboration between NIST and Stanford University, to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice. GIAB has authoritatively characterized human genome Reference Materials for use in analytical validation and technology development, optimization, and demonstration. In 2015, NIST released the pilot genome Reference Material 8398, which is genomic DNA derived from a large batch of a cell line and characterized for high-confidence small variant and homozygous reference regions (Zook, et al., Nature Biotechnology 2014).

There are four new GIAB reference materials available. With the addition of these new reference materials (RMs) to a growing collection of “measuring sticks” for genome sequencing, we can now provide laboratories with even more capability to accurately sequence DNA for genetic testing, medical diagnoses, and future customized drug therapies. The new tools feature sequenced genes from individuals in two genetically diverse groups, Asians and Ashkenazic Jews; a father-mother-child trio set from Ashkenazic Jews; and four microbes commonly used in research.

This suite of genome RMs provides medical and research laboratories worldwide with the tools they need to advance clinical applications of whole genome sequencing, and regulatory bodies with the ability to conduct science-based regulatory oversight of the technology.

Technical Contact: Justin Zook Email: [email protected]

https://www-s.nist.gov/srmors/view_detail.cfm?srm=8375 https://www-s.nist.gov/srmors/view_detail.cfm?srm=8391 https://www-s.nist.gov/srmors/view_detail.cfm?srm=8392 https://www-s.nist.gov/srmors/view_detail.cfm?srm=8393

http://www.nist.gov/srmhttp://jimb.stanford.edu/http://jimb.stanford.edu/https://www.nist.gov/news-events/news/2016/05/nist-and-stanford-launch-long-term-measurement-initiative-boost-us-biohttps://www.nist.gov/news-events/news/2016/05/nist-and-stanford-launch-long-term-measurement-initiative-boost-us-biohttps://www-s.nist.gov/srmors/view_detail.cfm?srm=8398http://www.nature.com/nbt/journal/v32/n3/full/nbt.2835.htmlhttps://www-s.nist.gov/srmors/view_detail.cfm?srm=8375https://www-s.nist.gov/srmors/view_detail.cfm?srm=8391https://www-s.nist.gov/srmors/view_detail.cfm?srm=8392https://www-s.nist.gov/srmors/view_detail.cfm?srm=8393



NIST RM 8671 NISTmAb, Humanized IgG1κ Monoclonal Antibody

Monoclonal antibodies account for 5 of the 10 top-selling drugs and over $75 billion in annual sales worldwide. Hundreds of monoclonal-antibody-based therapeutics are currently being evaluated for safety and effectiveness in clinical trials to treat grievous illnesses such as cancer and severe autoimmune disorders. Chosen for development in consultation with industry, NISTmAb RM 8671 is an important addition to the toolkits of biological drug manufacturers. The NISTmAb can be used to evaluate the performance of the manifold analytical and biophysical methods that are used to measure critical attributes of monoclonal antibodies. It also provides a representative test molecule for development of novel technology for therapeutic protein characterization.

Analytical and biophysical characterization spanning the entire structure continuum was collected using state-of-the art and emerging technologies to simultaneously establish a historical baseline dataset as well as confirm the NISTmAb’s industrial relevance (1-3). A resounding interest in a common, open innovation material was reinforced by these studies, and vialing was pursued to enable a longitudinally available, homogeneous, and stable reference material for public release. The NISTmAb RM 8671 has assigned reference values for concentration, charge purity, and size purity as well as informational values on protein particulate content and a comprehensive identity test. This RM may be useful for a variety of purposes including system suitability tests, establishing method or instrument performance and variability, comparing changing analytical test methods, and assisting in method qualification.

1. Schiel, J. E.; Davis, D. L.; Borisov, O. B., State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 1. Monoclonal Antibody Therapeutics: Structure, Function, and Regulatory Space. American Chemical Society: 2014; Vol. 1176, p 165.

2. Schiel, J. E.; Davis, D. L.; Borisov, O. B., State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 3. Defining the Next Generation of Analytical and Biophysical Techniques. American Chemical Society: 2015; Vol. 1202, p 455.

3. Schiel, J. E.; Davis, D. L.; Borisov, O. B., State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 2. Biopharmaceutical Characterization: The Nistmab Case Study. American Chemical Society: 2015; Vol. 1201, p 427.

Technical Contact: John Schiel Email: [email protected]


http://www.nist.gov/srmhttps://www-s.nist.gov/srmors/view_detail.cfm?srm=8671



NIST Speakers at PITTCON 2017 – March 6-9, 2017, Chicago, IL Date NIST Staff Event Title Time Location

Monday March 6

Lee Yu Assessment of Arsenic Species in Ginger/Chromatography and Sampling (TP0440)

9:10 am – 9:30 am

W177

Monday March 6

Anzor I. Mikaia

Gas Chromatography – Mass Spectrometry for Determination of Environmentally Important Phenols and Their Thio Analogs as Chemical Modification Products

10:00 am - 12:00 pm

Exposition Floor, Aisle 2500-2600

Monday March 6

Walter Wilson

Evaluation of Polycyclic Aromatic Hydrocarbon Standard Reference Material 2260a on Different Stationary Phases for Gas Chromatography, and Investigations of Retention Behavior of Polycyclic Aromatic Hydrocarbons and Polycyclic Aromatic Sulfur Heterocycles in Normal-Phase Liquid Chromatography

10:00 am – 12:00 pm

Exposition Floor Aisle 2500-2600

Monday March 6

Karen Phinney

Strategies for Protein Biomarker Quantification/Advances in Biomolecule Quantitation by Mass Spectrometry

4:10 pm – 4:45 pm

W178B

Tuesday March 7

Edward Sisco

Modifications of DART-MS for Enhanced Detection of Forensic Compounds/ Analysis of Drugs for Forensics Applications

9:30 am – 9:50 am

W175B

Wednesday March 8

Walter Wilson Determination of Polycyclic Aromatic Compounds and Their Alkyl-Substituted Derivatives in Combustion-Related Standard Reference Materials

8:50 am – 9:10 am

W175A

Wednesday March 8

Thomas Forbes

Real-Time Mass Spectrometry Detection of Remotely Sampled Vapors and Aerosols by Venturi-Assisted Entrainment and Ionization/Analysis of Explosives and Chemical Weapons for Forensics Applications (TP1910)

2:10 pm – 2:30 pm

W476

http://www.nist.gov/srm



NIST Speakers at PITTCON 2017 – March 6-9, 2017, Chicago, IL (cont.)

**NEW Ordering Process for SRMs 2627a, 2737, and 2738 - Nitric Oxide in Nitrogen **

Certification of the special cylinders is performed on a set schedule. The certification is valid for 2 years from the date of measurement. Please note that NIST will certify the units, prepare the documentation, and package the orders for shipment in the two months preceding the ship month. Orders received during that two-month period will be filled the following ship month.

Production schedule is as follows:

Orders placed in July, August, September, and October will ship in January; Orders placed in November, December, January, and February will ship in May; Orders placed in March, April, May, and June will ship in September.

Date NIST Staff Event Title

Time

Location

Wednesday March 8

Catherine Rimmer Analytical Methods and Reference Materials for Dietary Supplements

11:20 am - 11:50 am

W176C

Thursday March 9

Ashley Green

The Path Toward Urine Albumin Standardization/ Recognizing Cutting-Edge Chemistry from the National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChe) (TP2180)

9:45 am - 10:20 am

W181B

Thursday March 9

Marlon Walker

Metrology for ‘Stuff’ – and Its Impact on Innovation, Our Economic Security, and Quality of Life/Recognizing Cutting-Edge Chemistry from the NOBCChe

9:10 am – 9:45 am

W181B

Thursday March 9

Jason Streit

Scaling the Aqueous Two-Phase Separation of Carbon Nanotubes Through Countercurrent Chromatography/Nanotechnology Applications

1:30 pm – 1:50 pm

W476




Renewal SRMs/RMs

SRM 1473c Low Density Polyethylene Resin SRM 1474b Polyethylene Resin SRM 1667b Propane in Air (Nominal Amount-of-Substance Fraction 50 µmol/mol) Lot #83-K-XX SRM 3143 Rhenium (Re) Standard Solution SRM 3156 Tellurium (Te) Standard Solution SRM 3183 Fluoride Anion (F-) Standard Solution SRM 3184 Bromide Anion (Br-) Standard Solution SRM 3239 Isoflavones Calibration Solutions SRM 3262 St. John’s Wort (Hypericum perforatum L.) Aerial Parts SRM 8642a FDA Saxitoxin Dihydrochloride Solution Certificate Revisions This is a list of our most recent certificate revisions. NIST updates certificates for a variety of reasons, such as to extend the expiration date or to include additional information gained from stability testing. Certificates are the official source for values and expiration dates. Users of NIST Standard Reference Materials should ensure that they have the current certificates. You can print or view a copy of the current certificate at our website at http:/www.nist.gov/srm or contact the Office of Reference Materials at phone 301-975-2200, fax 301-926-4751, or email [email protected]

SRM 32e Carbon Low Alloy Steel (SAE 3140) Editorial changes SRM 173c Titanium Alloy UNS R56400 Editorial changes SRM 200b Potassium Dihydrogen Phosphate (Fertilizer Standard) Editorial changes SRM 361 AISI 4340 Steel (chip form) Editorial changes SRM 625 Zinc-Base Die-Casting Alloy A Editorial changes SRM 626 Zinc-Base Die-Casting Alloy B Editorial changes SRM 627 Zinc-Base Die-Casting Alloy C Editorial changes SRM 628 Zinc-Base Die-Casting Alloy D Editorial changes SRM 629 Zinc-Base Die-Casting Alloy E Editorial changes SRM 630 Zinc-Base Die-Casting Alloy F Editorial changes SRM 656 Silicon Nitride Powders (Quantitative Analysis Powder Diffraction Standard) Editorial changes

http://www.nist.gov/srmhttp://www.nist.gov/srmmailto:[email protected]



Revisions (continued)

SRM 861 Nickel-Base Superalloy PWA 1484 Editorial changes SRM 915b Calcium Carbonate Editorial changes SRM 971 Hormones in Frozen Human Serum Editorial changes SRM 1491a Methyl-Substituted Polycyclic Aromatic Hydrocarbons in Toluene New expiration date: 31 August 2026 Editorial changes SRM 1507b 11-Nor-Delta-9-Tetrahydrocannabinol-9-Carboxylic Acid in Freeze-Dried Urine New expiration date: 31 December 2026 Editorial changes SRM 1549a Whole Milk Powder Editorial changes SRM 1598a Inorganic Constituents in Animal Serum New expiration date: 01 October 2024 Editorial changes SRM 1649b Urban Dust Editorial changes SRM 1680b Carbon Monoxide in Nitrogen (Nominal Amount-of-Substance Fraction 500 µmol/mol) Lot #2-K-XX New expiration date: 20 September 2021 SRM 1878b Respirable Alpha Quartz (Quantitative X-Ray Powder Diffraction Standard) Editorial changes SRM 1881a Portland Cement (Blended with Slag and Fly Ash) New expiration date: 01 January 2018 Editorial changes SRM 1889a Portland Cement (Blended with Limestone) New expiration date: 01 March 2019 Editorial changes SRM 1980 Positive Electrophoretic Mobility Positive Electrophoretic Mobility (+µE) Standard Editorial changes SRM 1982 Thermal Spray Powder – Particle Size Distribution Yttria-Stabilized Zirconia (Spheroidal) Editorial changes SRM 2613a Carbon Monoxide in Air (Nominal Amount-of-Substance Fraction 20 µmol/mol) Lot #22-XX-E New expiration date: 02 May 2024 Editorial changes




Revisions (continued)

SRM 2683c Bituminous Coal (Nominal Mass Fraction 2 % Sulfur) New expiration date: 01 August 2019 Editorial changes SRM 2692c Bituminous Coal (Nominal Mass Fraction 1 % Sulfur) Editorial changes SRM 2693 Bituminous Coal (Nominal Mass Fraction 0.5 % Sulfur) New expiration date: 31 December 2021 Editorial changes SRM 2737 Nitric Oxide in Nitrogen (Nominal Amount-of-Substance Fraction 500 nmol/mol) Lot #2737-A-XX Editorial changes SRM 2738 Nitric Oxide in Nitrogen (Nominal Amount-of-Substance Fraction 1000 nmol/mol) Lot #2738-A-XX Editorial changes SRM 2772 B100 Biodiesel (Soy-Based) New expiration date: 28 February 2019 Editorial changes SRM 2773 B100 Biodiesel (Animal-Based) New expiration date: 28 February 2019 Editorial changes SRM 2786 Fine Atmospheric Particulate Matter (Mean Particle Diameter < 4 µm) Editorial changes SRM 2787 Fine Atmospheric Particulate Matter (Mean Particle Diameter < 10 µm) Editorial changes SRM 3155 Tantalum (Ta) Standard Solution New expiration date: 31 July 2020 Editorial changes SRM 3181 Sulfate Anion (SO42–) Standard Solution New expiration date: 30 November 2017 Editorial changes

SRM 3182 Chloride Anion (Cl–) Standard Solution New expiration date: 30 September 2018 Editorial changes

SRM 3287 Blueberry Fruit New expiration date: 01 December 2024 Editorial changes

SRM 4354 Freshwater Lake Sediment Environmental Radioactivity Standard Editorial changes




Revisions (continued) SRM 3184 Bromide Anion (Br-) Standard Solution New expiration date: 30 April 2028 RM 8393 Human DNA for Whole-Genome Variant Assessment (Son of Chinese Ancestry) Editorial changes

NIST 2017 SRM EXHIBIT SCHEDULE Pittsburgh Conference PITTCON Booth #4525 March 6-9, 2017 McCormick Place Chicago IL American Chemical Society Spring Meeting ACS Booth #1213-1215 April 2-6, 2017 Mascone Convention Center San Francisco, CA Materials Research Society Spring Meeting MRS Booth #217 April 17-21, 2017 Phoenix Convention Center Phoenix, AZ Clinical Lab Expo AACC Booth #911 July 30 - August 3, 2017 San Diego Convention Center San Diego, CA American Chemical Society Fall Meeting ACS Booth #2111-2113 August 20-24, 2017 Washington DC Convention Center Washington, DC 131st Annual Meeting & Exposition AOAC September 24-27, 2017 Marriott Atlanta Marquis Atlanta, GA

Materials Science & Technology Conference and Exhibit October 8-12, 2017 David L. Lawrence Convention Center Pittsburgh, PA Material Research Society Fall Meeting November 26 – December 1, 2017 Hynes Convention Center Boston, MA




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Documents

February 2017 · 2017. 2. 27. · Cigarette Tobacco Filler was prepared from air-cured very low nicotine tobacco that was processed using ... tobacco specific nitrosamines (TSNAs),