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First Phylogenetics Assignment:
Due Oct 27th (not Oct. 20th)
Horizontal Gene transfer
1. Atypical nucleotide compositionLawrence and Ochman paper
2. Presence/absence of genesWelch et al 2002
3. Incongruent Phlyogenies
Genetic exchange in bacteria/archaea
Transformation
Transduction
Conjugation
Often detected by atypical nucleotide composition
Detecting HGT from genomes: atypical nt composition
(Lawrence & Ochman, 1997)
Detecting HGT from genomes: atypical nt composition
Detecting HGT from genomes: atypical nt composition
Detecting HGT from genomes: atypical nt composition
(Lawrence & Ochman, 1998)
Molecular archaeology of the Escherichia coli genome
(Lawrence & Ochman, 1998)
Horizontal Gene transfer
1. Atypical nucleotide compositionLawrence and Ochman paper
2. Presence/absence of genesWelch et al 2002
3. Incongruent Phlyogenies
Detecting HGT from genomes: atypical nt composition
Detecting HGT: differential gene content
(Welch et al., 2002)
(Pál et al., 2005)
Horizontal Gene transfer
1. Atypical nucleotide compositionLawrence and Ochman paper
2. Presence/absence of genesWelch et al 2002
3. Incongruent Phlyogenies
Detecting HGT: incongruent phylogeny/synteny
HGT only Loss only
(Koonin, 2003)
Ex: glycerol-3-P DH
Detecting HGT: plants!?!
Rafflesia (Malpighiales)Rafflesia (Malpighiales)
(Davis & Wurdack, 2004)
Detecting HGT: bacteria to insects!?!
(Hottop et al., 2007)
Can gene histories be retraced?
(Xie et al., 2004)
Can gene histories be retraced?
(Kunin et al., 2005)
Is there still an organismal tree?
(Daubin et al., 2003)
Is there still an organismal tree?
(Daubin et al., 2003)
Limitations to HGT
Decrease in recombination w/ sequence divergence
(Fraser et al., 2007)
Bacillus subtilis
Bacillus mojavensis
Streptococcus pneumoniae
E. coli
Next week….Are there species? Or is it just a
continuum?